Organic liquid suspension fertilizer as well as preparation method and application thereof

Abstract

The invention provides an organic liquid suspension fertilizer as well as a preparation method and application thereof. Comprising the following components in parts by weight: 10-15 parts of chitosan mother liquor, 12-16 parts of amino acid fermentation mother liquor, 23-27 parts of gibberellin mushroom dreg fermentation liquor, 30-35 parts of vinasse fermentation liquor, 5-10 parts of potassium humate, 1-3 parts of a surfactant, 12-15 parts of a nitrogen fertilizer, 10-14 parts of a phosphate fertilizer, 26-31 parts of a potassium fertilizer, 3-8 parts of a trace element mixture and 35-40 parts of small molecule water. The organic liquid suspension fertilizer prepared by the invention can be used as a crop fertilizer, especially can improve the quality of potatoes and the yield and sugar content of beet, and reduces brown spot.

Description

Technical Field

[0001] The invention belongs to the technical field of fertilizers, and in particular relates to an organic liquid suspension fertilizer and a preparation method and application thereof. Background Art

[0002] In China, the main beet growing areas include Heilongjiang, Jilin, Shanxi and other provinces, as well as the high-altitude mountainous areas in the south. Due to the long-term use of chemical fertilizers in my country, the sustainable production capacity of the soil in agricultural production has declined, the soil quality has declined, and the beet production has shown a downward trend.

[0003] Organic liquid suspension fertilizer is a form of fertilizer that dissolves or disperses organic matter in a liquid medium. The characteristic of this fertilizer is that the insoluble organic matter is processed through a certain process to form a stable suspension system in the liquid. The advantages of this type of fertilizer include easy application and rapid absorption and utilization by plants. The liquid fertilizers currently on the market are not ideal for beets and have poor long-term storage stability.

[0004] Therefore, there is an urgent need for an organic liquid suspended fertilizer and a preparation method and application thereof. Summary of the invention

[0005] The purpose of the present invention is to provide an organic liquid suspended fertilizer and a preparation method and application thereof.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] An organic liquid suspended fertilizer comprises the following components in parts by weight: 10-15 parts of chitosan mother liquor, 12-16 parts of amino acid yeast liquid, 23-27 parts of gibberellin residue fermentation liquid, 30-35 parts of wine lees fermentation liquid, 5-10 parts of potassium humate, 1-3 parts of surfactant, 12-15 parts of nitrogen fertilizer, 10-14 parts of phosphorus fertilizer, 26-31 parts of potassium fertilizer, 3-8 parts of trace element mixture and 35-40 parts of small molecule water.

[0008] Furthermore, the preparation method of chitosan mother solution is to mix chitosan and water in a weight ratio of 1: (8-10), stir evenly, and obtain chitosan mother solution.

[0009] Furthermore, the method for preparing the gibberellin residue fermentation liquid comprises the following steps:

[0010] (1) adding water to the gibberellin residue to obtain a mixed liquid;

[0011] (2) adding a complex enzyme to the mixed liquid, performing enzymolysis at 30-35° C. for 4-7 hours, and after inactivating the enzyme, obtaining an enzymolysis product;

[0012] (3) adding composite bacteria to the enzymatic hydrolyzate, performing anaerobic fermentation at 38-40° C. for 15-20 h, sterilizing, and obtaining a fermentation product;

[0013] (4) placing the fermentation product into a microwave reactor for microwave extraction, centrifuging, collecting the liquid, and drying to obtain the gibberellin residue fermentation liquid.

[0014] Furthermore, the complex enzyme includes cellulase, pectinase and ficin; the amount of cellulase in the mixed liquid is 130-140 U / mL; the amount of pectinase in the mixed liquid is 100-110 U / mL; the amount of ficin in the mixed liquid is 80-90 U / mL.

[0015] Furthermore, the composite bacteria include Lactobacillus helveticus, Bifidobacterium longum and Bifidobacterium lactis. The amount of Lactobacillus helveticus in the enzymatic hydrolysate is 10 6 -10 7 CFU / mL; the amount of Bifidobacterium longum in the enzymatic hydrolysate was 10 7 -10 8 CFU / mL; the amount of Bifidobacterium lactis in the enzymatic hydrolysate was 10 8 -10 9 CFU / mL;

[0016] Furthermore, the conditions for microwave extraction are: microwave power 250-280W, temperature 70-75°C, and extraction time 1.5-2h.

[0017] In the production process of gibberellins, after fermentation, gibberellins need to be separated from the culture medium. In this process, a large amount of solid residues will remain, and this part of the residue is the gibberellins residue. The system of the present invention adds the gibberellins residue fermentation liquid obtained by enzymolysis and fermentation of the gibberellins residue in turn, which can increase the yield of sugar beets. The analysis shows that the gibberellins residue fermentation liquid after enzymolysis and fermentation contains more abundant nutrients, which can effectively promote the growth of sugar beets. And it is found that when specific enzymes and specific strains are used for enzymolysis and fermentation, the sugar content in sugar beet roots can be increased. The analysis shows that the organic matter in the residue can be converted into a form that is more easily utilized by plants through enzymolysis, which increases the nutrient content in the fermentation liquid. The plant growth regulators produced during the fermentation process can promote the growth of sugar beets, especially promote the expansion of tubers and sugar accumulation, and the sugar content in sugar beet roots can be increased through synergistic effects.

[0018] Furthermore, the method for preparing the wine lees fermentation liquid comprises the following steps:

[0019] (1) drying the wet vinasse to a water content of 10-13 wt %, and crushing the vinasse to a particle size of less than 100 meshes to obtain vinasse powder;

[0020] (2) mixing distiller's grains powder and water at a weight ratio of 1: (7-10) to obtain distiller's grains liquid, adding Lactobacillus bulgaricus to the distiller's grains liquid, and performing anaerobically fermentation at 38-40° C. for 14-16 hours. After sterilization, continuously adding Streptococcus thermophilus, and performing anaerobically fermentation at 38-40° C. for 25-28 hours. After sterilization, continuously adding Lactobacillus plantarum, and performing anaerobically fermentation at 38-40° C. for 5-10 hours. After sterilization, obtaining a fermentation filtrate;

[0021] (3) Centrifuging the fermentation filtrate, collecting the liquid, and adjusting it to neutrality to obtain distiller's grains fermentation liquid.

[0022] Furthermore, the amount of Lactobacillus bulgaricus used in the wine lees liquid is 10 6 -10 7 CFU / mL; the amount of thermophilic Streptococcus used in the wine lees liquid is 10 9 -10 10 CFU / mL; the amount of Lactobacillus plantarum used in the wine lees liquid is 10 8 -10 9 CFU / mL.

[0023] Beet brown spot is a disease caused by a fungus called Cercospora, which is common in beet crops. This disease mainly affects the leaves of beets, and in severe cases it can cause premature aging of the leaves, thereby affecting the yield and quality of beets. The present invention can improve the brown spot of beets by adding distiller's grains fermentation liquid to the organic liquid fertilizer. Analysis shows that distiller's grains are a by-product produced in the winemaking process, which are usually rich in protein, cellulose and other organic matter. Through the fermentation treatment of specific strains, the complex organic matter in the distiller's grains is decomposed and more nutrients are released. The organic matter in the fermentation liquid can provide additional nutrition for the beets, enhance their growth potential, and improve their disease resistance; the organic matter in the fermentation liquid can improve the soil structure, increase the air permeability and water retention of the soil, and is beneficial to the development of the root system of the beet; certain substances produced during the fermentation process (such as antibiotic substances) may have an antibacterial effect and reduce the number of pathogens.

[0024] Furthermore, the surfactant is a mixture of sodium dodecyl sulfate, monoglycerol fatty acid ester and sodium lignin sulfonate in a weight ratio of 1: (1.2-1.5): (0.5-0.7).

[0025] The components of the invention are reasonably proportioned, and the organic liquid suspended fertilizer prepared by simultaneously adding gibberellin residue fermentation liquid, distiller's grains fermentation liquid and a surfactant in a specific proportion has high stability, is not easy to be stratified and precipitated, and is conducive to storage.

[0026] Furthermore, small molecule water is prepared by using ultraviolet enhanced ionization to generate small-particle hydrated negative ions. For details, please refer to application number 202010337543.8, patent name: A device for generating small-particle hydrated negative ions by using ultraviolet enhanced ionization.

[0027] The invention provides a method for preparing the organic liquid suspended fertilizer, which comprises the following steps: mixing the components, grinding with a sand mill or a colloid mill until the particle size of the suspended matter is less than 0.05 mm, and obtaining the organic liquid suspended fertilizer.

[0028] The invention provides application of the organic liquid suspended fertilizer.

[0029] It should be noted that the organic liquid fertilizer of the present invention can be used for crops other than sugar beets, and has good use effects, which is of great significance for increasing crop yields.

[0030] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0031] 1. The system of the present invention adds the fermentation liquid of gibberellin residue obtained by enzymatic hydrolysis and fermentation of gibberellin residue to increase the yield of beets.

[0032] 2. When gibberellin residue is hydrolyzed and fermented using specific enzymes and specific bacteria, the sugar content in beet roots can be increased.

[0033] 3. The present invention can improve the brown spot disease of sugar beet by adding wine lees fermentation liquid to the organic liquid fertilizer.

[0034] 4. The components of the present invention are reasonably proportioned, and the organic liquid suspension fertilizer prepared by simultaneously adding gibberellin residue fermentation liquid, distiller's grains fermentation liquid and a surfactant in a specific proportion has high stability.

[0035] 5. The organic liquid suspension fertilizer prepared by the present invention can increase the yield and sugar content of sugar beets and reduce brown spot disease. At the same time, it can improve the yield and quality of potatoes, which is of great significance for potato planting and has very good effects in practical applications. DETAILED DESCRIPTION

[0036] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] The raw materials used in the following examples of the present invention are all commercially available products:

[0038] Amino acid yeast liquid, Jiangxi Muyu Ren Agricultural Biotechnology Co., Ltd., product name: amino acid fermentation liquid.

[0039] Chitosan, Shaanxi Baichuan Biotechnology Co., Ltd.

[0040] Gibberellic acid residue, Guangdong Mingtong Biotechnology Co., Ltd.

[0041] Wine lees, Weifang Jiahongyuan Biotechnology Co., Ltd., Luzhou-flavor liquor lees.

[0042] Potassium humate, Shanxi Jiayou Humic Acid Technology Co., Ltd.

[0043] Nitrogen fertilizer, urea.

[0044] Phosphate fertilizer, monoammonium phosphate.

[0045] Potash fertilizer, potassium sulfate.

[0046] The trace element mixture is a mixture of zinc sulfate, manganese sulfate and ammonium molybdate in a ratio of 1:2:1 by weight.

[0047] Monoglycerol fatty acid esters, Hebei Jiuxing Chemical Products Co., Ltd.

[0048] Lactobacillus plantarum was purchased from China Center for Type Culture Collection, strain number: CCTCC AB 2010210.

[0049] Lactobacillus bulgaricus was purchased from China Center for Type Culture Collection, strain number: CCTCC CB 20082295.

[0050] Streptococcus thermophilus was purchased from China Center for Type Culture Collection, strain number: CCTCC AB 2010206.

[0051] Lactobacillus helveticus was purchased from China Center for Type Culture Collection, strain number: CCTCC AB 2010205.

[0052] Bifidobacterium longum was purchased from China Center for Type Culture Collection, strain number: CCTCC HB 20082718.

[0053] Bifidobacterium lactis was purchased from Shanghai Microbiological Collection Center, strain number: SHBCC D24675.

[0054] Cellulase, pectinase, ficin, neutral protease, β-glucosidase and bromelain were purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0055] Example 1

[0056] The present embodiment provides an organic liquid suspended fertilizer, comprising the following components in parts by weight: 12 parts of chitosan mother liquor, 14 parts of amino acid yeast solution, 25 parts of gibberellin residue fermentation liquid, 33 parts of wine lees fermentation liquid, 7 parts of potassium humate, 2 parts of surfactant, 14 parts of nitrogen fertilizer, 12 parts of phosphate fertilizer, 28 parts of potassium fertilizer, 5 parts of trace element mixture and 37 parts of small molecule water.

[0057] The surfactant is a mixture of sodium dodecyl sulfonate, monoglycerol fatty acid ester and sodium lignin sulfonate in a weight ratio of 1:1.4:0.6.

[0058] The preparation method of chitosan mother solution is as follows: chitosan and water are mixed in a weight ratio of 1:9, and stirred evenly to obtain chitosan mother solution.

[0059] The preparation method of the gibberellin residue fermentation liquid comprises the following steps:

[0060] (1) adding water to the gibberellin residue to obtain a mixed liquid;

[0061] (2) adding a composite enzyme to the mixed liquid, performing enzymolysis at 32° C. for 5 h, and obtaining an enzymolysis product after inactivating the enzyme; the composite enzyme comprises cellulase, pectinase and ficin; the amount of the cellulase in the mixed liquid is 135 U / mL; the amount of the pectinase in the mixed liquid is 105 U / mL; and the amount of the ficin in the mixed liquid is 85 U / mL.

[0062] (3) Adding composite bacteria to the enzymatic hydrolysate, anaerobic fermentation at 39°C for 17 hours, sterilization, and obtaining a fermentation product; the composite bacteria include Lactobacillus helveticus, Bifidobacterium longum and Bifidobacterium lactis. The amount of Lactobacillus helveticus in the enzymatic hydrolysate is 10 6 CFU / mL; the amount of Bifidobacterium longum in the enzymatic hydrolysate was 10 7 CFU / mL; the amount of Bifidobacterium lactis in the enzymatic hydrolysate was 10 8 CFU / mL;

[0063] (4) The fermentation product was placed in a microwave reactor for microwave extraction. The microwave extraction conditions were as follows: microwave power 260 W, temperature 72° C., and extraction time 1.7 h. The product was centrifuged, the liquid was collected, and dried to obtain the gibberellin residue fermentation liquid.

[0064] The preparation method of the wine lees fermentation liquid comprises the following steps:

[0065] (1) drying the wet vinasse to a water content of 12 wt %, and crushing the vinasse to a particle size of less than 100 meshes to obtain vinasse powder;

[0066] (2) After mixing distiller's grains powder and water in a weight ratio of 1:8, a distiller's grains liquid is obtained, Lactobacillus bulgaricus is added to the distiller's grains liquid, and anaerobically fermented at 39° C. for 15 hours. After sterilization, Streptococcus thermophilus is continuously added, and anaerobically fermented at 39° C. for 27 hours. After sterilization, Lactobacillus plantarum is continuously added, and anaerobically fermented at 39° C. for 7 hours. After sterilization, a fermentation filtrate is obtained. The amount of Lactobacillus bulgaricus used in the distiller's grains liquid is 10 7 CFU / mL; the amount of thermophilic Streptococcus used in the wine lees liquid is 10 9 CFU / mL; the amount of Lactobacillus plantarum used in the wine lees liquid is 10 8 CFU / mL.

[0067] (3) Centrifuging the fermentation filtrate, collecting the liquid, and adjusting it to neutrality to obtain distiller's grains fermentation liquid.

[0068] Small molecule water is produced by using ultraviolet enhanced ionization to produce small-particle hydrated negative ions. For details, please refer to application number 202010337543.8 and patent name: A device for producing small-particle hydrated negative ions by using ultraviolet enhanced ionization.

[0069] The preparation method of the organic liquid suspended fertilizer comprises the following steps: mixing the components, grinding with a sand mill or a colloid mill until the particle size of the suspended matter is less than 0.05 mm, and obtaining the organic liquid suspended fertilizer.

[0070] Example 2

[0071] The present embodiment provides an organic liquid suspended fertilizer, comprising the following components in parts by weight: 10 parts of chitosan mother liquor, 16 parts of amino acid yeast solution, 27 parts of gibberellin residue fermentation liquid, 30 parts of wine lees fermentation liquid, 10 parts of potassium humate, 1 part of surfactant, 15 parts of nitrogen fertilizer, 10 parts of phosphate fertilizer, 31 parts of potassium fertilizer, 3 parts of trace element mixture and 40 parts of small molecule water.

[0072] The surfactant is a mixture of sodium dodecyl sulfonate, monoglycerol fatty acid ester and sodium lignin sulfonate in a weight ratio of 1:1.2:0.5.

[0073] The preparation method of chitosan mother solution is as follows: chitosan and water are mixed in a weight ratio of 1:8, and stirred evenly to obtain chitosan mother solution.

[0074] The preparation method of the gibberellin residue fermentation liquid comprises the following steps:

[0075] (1) adding water to the gibberellin residue to obtain a mixed liquid;

[0076] (2) adding a composite enzyme to the mixed liquid, performing enzymolysis at 30° C. for 7 h, and obtaining an enzymolysis product after inactivating the enzyme; the composite enzyme comprises cellulase, pectinase and ficin; the amount of the cellulase in the mixed liquid is 130 U / mL; the amount of the pectinase in the mixed liquid is 110 U / mL; and the amount of the ficin in the mixed liquid is 80 U / mL.

[0077] (3) Adding composite bacteria to the enzymatic hydrolysate, anaerobic fermentation at 40°C for 15 hours, sterilization, and obtaining a fermentation product; the composite bacteria include Lactobacillus helveticus, Bifidobacterium longum and Bifidobacterium lactis. The amount of Lactobacillus helveticus in the enzymatic hydrolysate is 10 7 CFU / mL; the amount of Bifidobacterium longum in the enzymatic hydrolysate was 10 7 CFU / mL; the amount of Bifidobacterium lactis in the enzymatic hydrolysate was 10 9 CFU / mL;

[0078] (4) The fermentation product was placed in a microwave reactor for microwave extraction. The microwave extraction conditions were as follows: microwave power 250 W, temperature 75° C., and extraction time 1.5 h. The product was centrifuged, the liquid was collected, and dried to obtain the gibberellin residue fermentation liquid.

[0079] The preparation method of the wine lees fermentation liquid comprises the following steps:

[0080] (1) drying the wet vinasse to a water content of 13 wt %, and crushing the vinasse to a particle size of less than 100 meshes to obtain vinasse powder;

[0081] (2) After mixing distiller's grains powder and water in a weight ratio of 1:10, a distiller's grains liquid is obtained, Lactobacillus bulgaricus is added to the distiller's grains liquid, and anaerobically fermented at 38°C for 16 hours. After sterilization, thermophilic streptococci are continuously added, and anaerobically fermented at 38°C for 28 hours. After sterilization, Lactobacillus plantarum is continuously added, and anaerobically fermented at 40°C for 5 hours. After sterilization, a fermentation filtrate is obtained. The amount of Lactobacillus bulgaricus used in the distiller's grains liquid is 10 6 CFU / mL; the amount of thermophilic Streptococcus used in the wine lees liquid is 10 10 CFU / mL; the amount of Lactobacillus plantarum used in the wine lees liquid is 10 9 CFU / mL.

[0082] (3) Centrifuging the fermentation filtrate, collecting the liquid, and adjusting it to neutrality to obtain distiller's grains fermentation liquid.

[0083] Small molecule water is produced by using ultraviolet enhanced ionization to produce small-particle hydrated negative ions. For details, please refer to application number 202010337543.8 and patent name: A device for producing small-particle hydrated negative ions by using ultraviolet enhanced ionization.

[0084] The preparation method of the organic liquid suspended fertilizer comprises the following steps: mixing the components, grinding with a sand mill or a colloid mill until the particle size of the suspended matter is less than 0.05 mm, and obtaining the organic liquid suspended fertilizer.

[0085] Comparative Example 1

[0086] The difference between this comparative example and Example 1 is: an organic liquid suspended fertilizer, comprising the following components in parts by weight: 7 parts of chitosan mother liquor, 19 parts of amino acid yeast solution, 33 parts of gibberellin residue fermentation liquid, 25 parts of wine lees fermentation liquid, 7 parts of potassium humate, 2 parts of surfactant, 14 parts of nitrogen fertilizer, 12 parts of phosphate fertilizer, 28 parts of potassium fertilizer, 5 parts of trace element mixture and 37 parts of small molecule water.

[0087] Comparative Example 2

[0088] The difference between this comparative example and Example 1 is that the composite enzyme includes neutral protease, β-glucosidase and bromelain; the amount of the neutral protease in the mixed liquid is 135 U / mL; the amount of the β-glucosidase in the mixed liquid is 105 U / mL; the amount of bromelain in the mixed liquid is 85 U / mL.

[0089] Comparative Example 3

[0090] The difference between this comparative example and Example 1 is that the composite bacteria include Lactobacillus plantarum, Lactobacillus bulgaricus and Streptococcus thermophilus. The amount of Lactobacillus plantarum in the enzymatic hydrolysate is 10 6 CFU / mL; the amount of Lactobacillus bulgaricus in the enzymatic hydrolysate was 10 7 CFU / mL; the amount of thermophilic streptococcus in the enzymatic hydrolysate is 10 8 CFU / mL.

[0091] Comparative Example 4

[0092] The difference between this comparative example and Example 1 is that the bacterial species are different.

[0093] The preparation method of the wine lees fermentation liquid comprises the following steps:

[0094] (1) drying the wet vinasse to a water content of 12 wt %, and crushing the vinasse to a particle size of less than 100 meshes to obtain vinasse powder;

[0095] (2) After mixing distiller's grains powder and water in a weight ratio of 1:8, a distiller's grains liquid is obtained, Lactobacillus helveticus is added to the distiller's grains liquid, and anaerobic fermentation is carried out at 39° C. for 15 h, Bifidobacterium longum is continued, and anaerobic fermentation is carried out at 39° C. for 27 h, Bifidobacterium lactis is continued to be added, and anaerobic fermentation is carried out at 39° C. for 7 h, and sterilization is carried out to obtain a fermentation filtrate; the amount of Lactobacillus helveticus used in the distiller's grains liquid is 10 7CFU / mL; the amount of Bifidobacterium longum used in the wine lees liquid is 10 9 CFU / mL; the amount of Bifidobacterium lactis used in the wine lees liquid is 10 8 CFU / mL.

[0096] (3) Centrifuging the fermentation filtrate, collecting the liquid, and adjusting it to neutrality to obtain distiller's grains fermentation liquid.

[0097] Comparative Example 5

[0098] The difference between this comparative example and Example 1 is that the surfactant is a mixture of sodium dodecyl sulfate, monoglycerol fatty acid ester and sodium lignin sulfonate in a weight ratio of 1:1:1.

[0099] Comparative Example 6

[0100] The difference between this comparative example and Example 1 is that the surfactant is a mixture of Tween 60, Span 80 and sodium dodecyl sulfate in a weight ratio of 1:1.4:0.6.

[0101] Performance Testing

[0102] 1. An experimental field was selected in Nehe City, Heilongjiang Province, and the organic liquid suspension fertilizer prepared in Example 1-2 and Comparative Example 6 was applied to sugar beet. One mu was selected for each experimental group, and three parallels were made for each experimental group. The fertilizers were applied during the lush leaf cluster period and the tuber growth period, respectively, with an application amount of 10 kg / mu / time. The yield of sugar beet was counted, and the sugar content of the tubers and the brown spot disease grade of the sugar beet were measured using a sugar beet quality automatic analyzer. The brown spot disease grade standard is: Grade 0: no disease or a few plants have a few spots; Grade 1: Most plants have a few spots or a few plants have a majority of spots; Grade 2: Most plants have a majority of spots, and less than a quarter of the outer leaves die due to disease; Grade 3: Most plants have a majority of spots, and a quarter to two quarters of the outer leaves die due to disease; Grade 4: Most of the leaves of the plants die due to disease.

[0103] 2. The organic liquid suspension fertilizers prepared in Example 1-2 and Comparative Example 6 were stored at room temperature (25°C) in the dark for 6 months to observe whether precipitation or stratification occurred. If precipitation or / and stratification occurred, it was recorded as failure; if neither precipitation nor stratification occurred, it was recorded as passing.

[0104] The results are shown in Table 1.

[0105] Table 1 Test results of non-organic liquid suspension fertilizer

[0106]

[0107]

[0108] From the above performance test results, it can be seen that the organic liquid suspension fertilizers of Examples 1-2 can increase the yield and sugar content of sugar beets and reduce brown spot disease. In particular, the comprehensive performance of Example 1 is the most outstanding, which is mainly due to the synergistic effect between the components.

[0109] However, the comparative examples were significantly inferior to the embodiments in the corresponding performance tests because they did not adopt the necessary technical solutions. In comparative example 1, the ratio of the components was changed, and it can be seen that the effect of the organic liquid suspension fertilizer decreased, proving that the components work synergistically. The enzymes and strains used in the preparation of gibberellin residue fermentation liquid in comparative examples 2 and 3 were changed, resulting in a decrease in the sugar content in the beetroots. The strains used in the preparation of vinasse fermentation liquid in the comparative examples were changed, and the brown spot disease of the beets was aggravated. Comparative examples 5-6 changed the ratio of the surfactant, and the stability of the prepared organic liquid suspension fertilizer decreased. The above experimental results further prove the importance of the technical solutions defined in the present invention for its technical effects.

[0110] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. An organic liquid suspension fertilizer, characterized in that: The invention comprises the following components in parts by weight: 10-15 parts of chitosan mother liquor, 12-16 parts of amino acid yeast liquid, 23-27 parts of gibberellin residue fermentation liquid, 30-35 parts of wine lees fermentation liquid, 5-10 parts of potassium humate, 1-3 parts of surfactant, 12-15 parts of nitrogen fertilizer, 10-14 parts of phosphorus fertilizer, 26-31 parts of potassium fertilizer, 3-8 parts of trace element mixture and 35-40 parts of small molecule water.

2. The organic liquid suspension fertilizer according to claim 1, characterized in that: The preparation method of the gibberellin residue fermentation liquid comprises the following steps: (1) adding water to the gibberellin residue to obtain a mixed liquid; (2) adding a complex enzyme to the mixed liquid, performing enzymolysis at 30-35° C. for 4-7 hours, and after inactivating the enzyme, obtaining an enzymolysis product; (3) adding composite bacteria to the enzymatic hydrolyzate, performing anaerobic fermentation at 38-40° C. for 15-20 h, sterilizing, and obtaining a fermentation product; (4) placing the fermentation product into a microwave reactor for microwave extraction, centrifuging, collecting the liquid, and drying to obtain the gibberellin residue fermentation liquid.

3. The organic liquid suspension fertilizer according to claim 2, characterized in that: The complex enzyme comprises cellulase, pectinase and ficin; the amount of the cellulase in the mixed liquid is 130-140 U / mL; the amount of the pectin in the mixed liquid is 100-110 U / mL; and the amount of the ficin in the mixed liquid is 80-90 U / mL.

4. The organic liquid suspension fertilizer according to claim 2, characterized in that: The composite bacteria include Lactobacillus helveticus, Bifidobacterium longum and Bifidobacterium lactis.

5. The organic liquid suspension fertilizer according to claim 2, characterized in that: The conditions of microwave extraction are: microwave power 250-280W, temperature 70-75°C, and extraction time 1.5-2h.

6. The organic liquid suspension fertilizer according to claim 1, characterized in that: The preparation method of the wine lees fermentation liquid comprises the following steps: (1) drying the wet vinasse to a water content of 10-13 wt %, and crushing the vinasse to a particle size of less than 100 meshes to obtain vinasse powder; (2) mixing distiller's grains powder and water at a weight ratio of 1: (7-10) to obtain distiller's grains liquid, adding Lactobacillus bulgaricus to the distiller's grains liquid, and performing anaerobically fermentation at 38-40° C. for 14-16 hours. After sterilization, continuously adding Streptococcus thermophilus, and performing anaerobically fermentation at 38-40° C. for 25-28 hours. After sterilization, continuously adding Lactobacillus plantarum, and performing anaerobically fermentation at 38-40° C. for 5-10 hours. After sterilization, obtaining a fermentation filtrate; (3) Centrifuging the fermentation filtrate, collecting the liquid, and adjusting it to neutrality to obtain distiller's grains fermentation liquid.

7. The organic liquid suspension fertilizer according to claim 6, characterized in that: The amount of Lactobacillus bulgaricus used in the wine lees liquid is 10 6 -10 7 CFU / mL; the amount of thermophilic Streptococcus used in the wine lees liquid is 10 9 -10 10 CFU / mL; the amount of Lactobacillus plantarum used in the wine lees liquid is 10 8 -10 9 CFU / mL.

8. The organic liquid suspension fertilizer according to claim 1, characterized in that: Small molecule water is produced by using ultraviolet enhanced ionization to produce small particle size hydrated negative ions.

9. The method for preparing the organic liquid suspension fertilizer according to any one of claims 1 to 8, characterized in that: The preparation method comprises the following steps: mixing the components, grinding with a sand mill or a colloid mill until the particle size of the suspended matter is less than 0.05 mm, and obtaining an organic liquid suspended fertilizer.

10. Use of the organic liquid suspension fertilizer according to any one of claims 1 to 8.

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