82 results about "Medicago" patented technology

The invention relates to an ecological restoration method for a high and cold mine, and belongs to the technical field of ecological restoration. The ecological restoration method for the high-cold mine comprises the step of performing ecological restoration on the high-cold mine by adopting vegetation configuration of shrubs, herbaceous plants A and herbaceous plants B, the herbaceous plants A comprise at least four of elymus dahuricus, siberian wildrye herb, festuca arundinacea, bluegrass, stipa purpurea, ryegrass, frozen artemisia stelleriana and herba artemisiae annuae; the herbaceous plant B comprises at least three of artemisia desertorum, plantain herb, alfalfa, astragalus membranaceus, dandelion and oxytropis philippinarum; the shrub species comprise at least two of sophora moorcroftiana, sabina vulgaris, sea-buckthorn, caragana and buddleia. According to the method, specific vegetation configuration of shrubs, herbaceous plants A and herbaceous plants B is adopted, and soil microorganism resources are recovered quickly; the extreme environment of high and cold mines can be overcome; the ecological system is quickly and efficiently restored, only one-time sowing is needed, and the cost is low; the vegetation and microorganisms interact with each other, so that the survival rate of the vegetation is high.

The invention relates to the technical field of saline-alkali soil treatment, and discloses a light and moderate saline-alkali soil alfalfa seedling protection and strong growth promotion planting method which comprises the following steps: S1, soil pretreatment and root zone microenvironment construction; s2, seed treatment and space-time double-salt-avoiding sowing; s3, microbial inoculum synergistic application; s4, water and fertilizer integration and accurate management; s5, dynamic regulation and control in the growth period, a low-salt root zone microenvironment is constructed through combination of physical obstruction and biological improvement, salt ascending is inhibited by using a slag salt isolation layer, fertility is improved by using an organic layer, and stress resistance is enhanced by adopting a salt-tolerant variety, climate window sowing and aerogel embedding fungicide; precise regulation and control are achieved through water and fertilizer integrated drip irrigation, the stability of a root zone is maintained by combining intertillage and directional topdressing, finally, the seedling emergence uniformity and the seedling stage survival rate are remarkably improved, and robust growth of plants in saline-alkali soil is promoted.

The invention discloses a method for detecting verticillium medicaginis based on an enzyme-mediated double-index amplification technology and a detection kit of the verticillium medicaginis. The invention belongs to the field of biological detection, and particularly relates to a method for detecting verticillium medicaginis based on an enzyme-mediated double-index amplification technology and a detection kit of the verticillium medicaginis. The composition for detecting medicago sativa verticillium comprises a primer F1, a primer R1 and an RNA probe, the nucleotide sequence of the primer F1 is as shown in SEQ ID No: 1, the nucleotide sequence of the primer R1 is as shown in SEQ ID No: 7, and the nucleotide sequence of the RNA probe is as shown in SEQ ID No: 15. The rapid visual detection technology for the verticillium medicaginis EnDEA, which is researched and developed by the invention, is high in specificity, rapid and sensitive, and convenient for detection personnel to carry out rapid detection on the spot, so that the detection time is greatly shortened, the detection cost is reduced, and an important reference is provided for rapid detection of the verticillium medicaginis.

The invention discloses an enterobacter strain and application thereof in improving stress resistance of plants. The invention relates to the field of biological agriculture, and provides Enterobacter sphaeroides YJD-AD2-2, and the enrollment number of the Enterobacter sphaeroides YJD-AD2-2 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms is CGMCC No.29524. The Enterobacter sphaeroides YJD-AD2-2 can be used for preparing the enterobacter sphaeroides YJD-AD2-2. The enterobacter sphaeroides YJD-AD2-2 provided by the invention can improve the resistance of arabidopsis thaliana, alfalfa, corn and rice to salt and alkali stress, specifically, the dry weight, fresh weight, plant length, leaf area and chlorophyll content are increased, the oxidation resistance is improved, wilt is reduced, and physiological and biochemical indexes are improved. The YJD-AD2-2 microbial inoculum improves the property and activity of saline alkali soil and improves the abundance and composition of microbial communities. Detection shows that the YJD-AD2-2 strain has the capabilities of secreting IAA (Indoleacetic Acid), ACC (Acetyl Cellulose) deaminase and siderophore, dissolving phosphorus and fixing nitrogen. The indexes show that the YJD-AD2-2 microbial inoculum has the potential of growth-promoting bacteria and has important application value in the field of agriculture.

The invention belongs to the technical field of soil remediation, and provides a preparation method of a saline-alkali soil remediation agent aiming at the problem of insufficient long-acting stability caused by uncontrollable Ca < 2 + > release of an existing saline-alkali soil remediation agent. The method comprises the following steps: by taking alfalfa residues as raw materials, fermenting to generate acidic fermentation liquor, dissolving soil carbonate, reducing pH, triggering coordinate bond dissociation of chelated calcium gel, realizing pH responsiveness of releasing Ca < 2 + >, and releasing Ca < 2 + > to replace Na < + >; meanwhile, quinonyl on the surface of the modified biochar is used for activating extracellular electron transfer of the Prussella adamsii, and hydroxyl ion secretion is promoted to neutralize salt groups; the alfalfa humification fermentation material and exopolysaccharides secreted by flora are crosslinked through hydrogen bonds to form a cementation layer, soil aggregates are stabilized through a modified charcoal pore framework, calcium slow release balance is regulated and controlled, the problem of local hardening caused by sudden release of Ca < 2 + > is cooperatively solved, and long-acting repair and improvement of the saline-alkali soil are achieved.

The invention relates to the field of biological agriculture, in particular to application of a gene MtUMAMIT8 to regulation and control of growth, development and quality of forage grass. The biological function of the gene MtUMAMIT8 is identified for the first time, the MtUMAMIT8 gene is screened and locked through multi-omics analysis, the phenotype of a homozygous mutant NF8465 / NF12630 is analyzed, it is proved that the growth of medicago truncatula is hindered but the lignin content is reduced due to down-regulation of the gene MtUMAMIT8, it is clear that the gene MtUMAMIT8 positively regulates the growth and development of medicago truncatula plant height, the branch number, the leaf size and the like and negatively regulates lignin synthesis, and the gene MtUMAMIT8 can be used for identifying the lignin content of the medicago truncatula. And a new gene resource is provided for regulating and controlling the growth, development and quality of forage grass and breeding forage grass varieties with low lignin content.

The application discloses a sequence of Medicago truncatula AP2 / ERF transcription factor ERM1 and application of the sequence in improving mycorrhizal colonization efficiency of leguminous plants and promoting mycorrhizal symbiosis, and relates to the technical field of plant genetic engineering. ERM1 The gene has a significant promoting effect on the symbiosis of alfalfa and arbuscular mycorrhizal fungi, and the application has important significance for clarifying the biological function of ERM1 in the symbiosis of leguminous plants and mycorrhizal fungi, thereby regulating the symbiosis between plants and mycorrhizal fungi, and provides a research foundation and scientific basis for improving the symbiosis efficiency of leguminous crops by using biological strengthening means.

The invention discloses a saline-alkali grassland management method based on ecological rotation and land sharing, which comprises the following steps: equally dividing a target land into a plurality of grid areas, collecting soil samples in different grid areas to measure the pH and total nitrogen content of soil, and dividing the soil into an ecological restoration area, a forage grass production area and an economic plant area according to the measured pH and total nitrogen content of the soil. Suaeda glauca and sesbania are planted in the ecological restoration area, alfalfa and leymus chinensis are planted in the forage grass production area, radix isatidis and purple perilla are planted in the economic plant area, and crop rotation planting is conducted on the three areas with two years as the cycle. According to the design, through complementation of plant communities, the fertilization requirement can be reduced, the soil remediation speed can be increased, grassland rotation management of multi-subject land sharing is achieved through ecological function complementation, the land utilization efficiency can be effectively improved, the grassland production-ecology-life three-generation functions are considered, and sustainable development of the saline-alkali grassland is promoted.

The invention belongs to the technical field of agricultural planting, and provides a method for interplanting triticale and alfalfa in barren land, which comprises the following steps: S1, selecting triticale and alfalfa varieties suitable for local environment; s2, in the period from September to October, soil deep ploughing is conducted on the planting land parcel by 25-30 cm, and triticale seeds and alfalfa seeds are evenly mixed according to the proportion of 7: 1; s3, harvesting triticale in different periods according to utilization purposes, and continuing to reserve alfalfa for growth after harvesting; s4, performing irrigation, disease and pest monitoring, fertilization, weeding and cutting management on the feed triticale and alfalfa; according to the method, crop varieties suitable for the local environment are selected, soil deep ploughing and seed treatment are conducted, reasonable sowing and fertilization are conducted, and fine field management measures are taken, so that efficient utilization of the barren land is achieved; according to the method, the utilization rate of land and the yield of crops are increased, diseases and insect pests are reduced through interplanting and fine management, the soil structure is improved, and the soil fertility is improved.

The application provides a kind of alfalfa population three-dimensional structure phenotype information extraction method and system. First, according to the ground and wall plane in the original point cloud, the original point cloud is corrected in coordinate;Then according to the reason and characteristics of noise generated by original point cloud, based on statistical filtering removes outlier, based on crown voxel filtering removes redundant point, based on moving least square method removes mixed point;Then according to the characteristics of planting and growth of potted alfalfa population, the alfalfa population is extracted by using straight-through filtering and grid division method, and the single plant is segmented, and then six kinds of structure parameters such as maximum plant height, average plant height, plant height quantile, canopy cover, projected leaf area and three-dimensional volume are extracted.The application realizes the lossless automatic extraction of alfalfa population structure phenotype parameters, and the application is also suitable for three-dimensional structure phenotype information extraction of other population growth plants.

This invention discloses a rapid establishment and high-frequency harvesting method for alfalfa cultivation on desert edges without the need for windbreaks, belonging to the field of sandy land ecological agriculture and forage cultivation technology. This method is suitable for shifting sandy areas on desert edges, eliminating the need for windbreaks. Early-maturing forage oats are sown in single rows in spring. After the oats reach a height of 10-20cm and form a windbreak barrier, drought- and salt-tolerant alfalfa is sown in rows between the rows. Drip or sprinkler irrigation is used, and nitrogen, phosphorus, and potassium fertilizers are reduced by 20%-30% compared to conventional methods. Three to five days after each alfalfa harvest, a nutrient solution containing enzymatically hydrolyzed animal-derived small molecule peptides with a molecular weight <1000Da is applied with irrigation water. The oats are harvested in one go after maturity; the alfalfa is harvested frequently when the plants reach a height of 50-60cm, with 5-6 harvests per year. This invention utilizes oats for temporary wind protection, resulting in a lower than 15% mortality rate from wind and sand during the alfalfa seedling stage, enabling planting in the same year. The hay has a crude protein content of 19%–22%, a yield of over 1200 kg of hay per mu, and can be harvested continuously for 8–10 years. It is suitable for desert edge areas with annual precipitation <100 mm.

The application discloses a method for improving productivity of alfalfa grassland in semi-arid area, which comprises the following steps: sowing in the same period strip sowing mode of planting one row of alfalfa every two rows of feed oat; harvesting mixed forage of alfalfa and feed oat only once in the first year, and not ploughing the land after mowing; in the second year, sowing two rows of feed oat in the alfalfa row without ploughing from late April to early May after the alfalfa turns green; harvesting mixed forage of alfalfa and feed oat in the first harvest in the second year; harvesting alfalfa grass in the second and third harvests; in the third year and the following years, sowing two rows of feed oat in the alfalfa row without ploughing after the alfalfa turns green from late April to early May; in the third year and the following years, harvesting mixed forage of alfalfa and feed oat in the first harvest at the initial flowering period of alfalfa in late June; and harvesting alfalfa grass in the second and third harvests. The application has high utilization rate of natural precipitation, does not need irrigation, has high yield, and can effectively improve the productivity of alfalfa grassland in semi-arid area.

A device for improving the germination rate of alfalfa under continuous cropping, relating to the field of seedling technology; comprising a shell, with seedling trays fixedly installed on the left and right sides of the shell from bottom to top, and a feed cylinder assembly that can move up, down, forward, and backward between the seedling trays inside the shell; the feed cylinder assembly includes a funnel-shaped feed cylinder, with an openable and closable cylinder cover at the top of the feed cylinder, and a rotatable rotating cylinder sleeved on the lower outer side of the feed cylinder, with a horizontal feed guide pipe integrally connected to the bottom of the rotating cylinder, and a feed dispensing through hole at the bottom of the feed guide pipe; a peeling cylinder is rotatably connected between the two ends of the feed guide cylinder, with a gap between the outer side of the peeling cylinder and the inner side of the feed guide cylinder, and a shaft connected between the two ends of the peeling cylinder, with opposing spiral blades connected on both sides of the shaft located at the inner center of the peeling cylinder, an axial discharge port on the outer side of the peeling cylinder, and uniformly distributed protrusions integrally connected to the outer side of the peeling cylinder; and an air conditioner installed on the outer side of the shell.

The invention discloses a cysteine-rich small peptide CYSTM5 gene of medicago truncatula and application of the cysteine-rich small peptide CYSTM5 gene, and provides a method for cultivating a cold-resistant and disease-resistant plant by using the MtCYSTM5 gene, and after the gene is over-expressed in medicago truncatula, the cold resistance and disease resistance of transgenic medicago truncatula are improved; meanwhile, after the gene is inserted into a mutant in medicago truncatula through Tnt1, the gene loses efficacy, and the cold resistance and disease resistance of transgenic medicago truncatula are reduced; and after a mutant plant is infected by a complementary mutant carrier, the cold resistance and disease resistance of the mutant can be recovered. The gene for regulating and controlling cold resistance and disease resistance of medicago truncatula is excavated, and a target gene is provided for cold-resistant and disease-resistant molecular breeding of other pasture or crops.

The application focuses on the field of agricultural informatization and remote sensing application technology, and particularly relates to a kind of alfalfa yield estimation method based on multispectral and physical constraint sample enhancement. The method first acquires unmanned aerial vehicle multispectral image data corresponding to the alfalfa growth period, and lays out field quadrats with equal area in the image coverage area, collects measured data of alfalfa hay yield, leaf area index, chlorophyll content and leaf equivalent water thickness, and constructs an initial sample set. Then, input each parameter data within a reasonable range, generate predicted multispectral samples that meet the physical consistency constraint using the radiation transfer model, to expand the sample space. The measured samples and predicted samples are fused to construct an alfalfa hay yield estimation training data set. Based on the training data set, a mapping model between unmanned aerial vehicle multispectral features and alfalfa hay yield is established, and the alfalfa hay yield is estimated through machine learning.

The invention discloses a pasture weed identification method based on a convolutional neural network and application thereof. The method comprises the following steps: respectively acquiring image data of an alfalfa field before sowing and when seedlings turn green; on the basis of alfalfa field image data before sowing, weeds are judged through color features; on the basis of alfalfa field image data when seedlings turn green, dividing weeds into monocotyledonous weeds and dicotyledonous weeds according to the types of the weeds, and performing key feature acquisition on alfalfa and dicotyledonous weeds; category labels are attached to the initial alfalfa and weeds based on the key features, and a training set and a test set are divided; and constructing a pasture weed identification model based on the convolutional neural network model, training the convolutional neural network through the training set, and selecting the convolutional neural network model of the corresponding parameter with the highest identification accuracy. The method has the advantages that the weeds are classified, key features are extracted for alfalfa and dicotyledonous weeds, and efficient and accurate recognition of the weeds in the alfalfa field is achieved through CNN model training.

The invention discloses an application of a CKX gene in regulating alfalfa root systems, comprising the following steps: step 1, preparing a transgenic wild-type alfalfa, overexpressing the MtCKX1 gene in the alfalfa, and obtaining a transgenic plant; the cDNA sequence and amino acid sequence of the MtCKX1 gene are sequence 1 and sequence 2 in the sequence table; step 2, comparing the root systems of the transgenic alfalfa plant with those of the wild-type plant, and the root systems of the transgenic alfalfa plant are larger than those of the wild-type plant; the cytokinin content of the transgenic plant is suppressed, and the root systems of the transgenic plant are strengthened and enlarged; cytokinin has a negative regulatory effect on the root growth of the plant, and after the MtCKX1 gene is overexpressed, the cytokinin is suppressed, and the root system of the plant is strengthened and enlarged, thereby effectively fixing the soil and preventing soil loss.

The invention discloses alfalfa seed propagation equipment and method, and belongs to the field of leguminous plant planting, the alfalfa seed propagation equipment comprises a base and further comprises a diffusion assembly, the diffusion assembly comprises a fixing plate fixedly installed on the upper portion of the base, and a discharging frame is fixedly installed on the lower portion of the base; a discharging opening communicated with the discharging frame is formed in the fixing plate, a movable cavity is formed in the inner cavity wall of the discharging opening, a gear ring is rotationally installed in the movable cavity, a partition plate is fixedly installed on the inner wall of the gear ring, and a connecting rod is fixedly connected to the bottom of the partition plate; according to the alfalfa seeding device, alfalfa seeds can be uniformly arranged in seeding pits, and the situation that the alfalfa seeds are seeded too densely to cause intense competition and influence the growth and development of alfalfa can be avoided, so that the consistency of the spacing between planted plants can be effectively improved, and the growth of the alfalfa and the balance of light, nutrients and moisture can be effectively improved.

The present invention discloses a saline-alkali grassland management method based on ecological rotation and land sharing. The target plot is first divided into multiple grid areas. Soil samples are collected from different grid areas to determine the soil pH and total nitrogen content. Then, based on the measured soil pH and total nitrogen content, the area is divided into an ecological restoration area, a forage production area, and an economic plant area. Salicornia and Sesbania are planted in the ecological restoration area, alfalfa and Leymus chinensis are planted in the forage production area, and Isatis indigotica and Perilla are planted in the economic plant area. Crops are rotated in the three areas on a two-year cycle. The above design can reduce fertilization requirements and increase soil remediation speed through the complementarity of plant communities. It can achieve multi-subject land sharing grassland rotation management through the complementarity of ecological functions, effectively improve land use efficiency, take into account the "three lives" of grassland production, ecology, and life, and promote the sustainable development of saline-alkali grassland.

This invention relates to a method for creating late-flowering, high-yielding alfalfa, characterized by the following steps: S1: designing and synthesizing targeted alfalfa MvFT S1: Construct the gRNA of the gene; S2: Construct a CRISPR / Cas9 gene knockout vector containing the gRNA expression cassette described in S1; S3: Transform the knockout vector described in S2 into Agrobacterium tumefaciens; S4: Transform explants of alfalfa cultivars into Agrobacterium tumefaciens as described in S3; S5: Screen, differentiate, and regenerate the explants transformed in S4 to obtain transgenic plants; S6: Perform molecular and phenotypic identification on the transgenic plants to screen for... MvFT A late-flowering, high-yielding germplasm with homozygous gene knockout and significantly delayed flowering time. This invention reveals for the first time... MvFT The key functions of genes in the regulation of flowering in alfalfa were investigated, and new late-flowering, high-yielding alfalfa germplasm was successfully created, providing valuable germplasm resources for breeding new high-yielding and high-quality alfalfa varieties.

The present application provides Bacillus subtilis LYZ1128, a biocontrol agent, and applications thereof. The deposit number of the Bacillus subtilis LYZ1128 is CGMCC No. 31719. The Bacillus subtilis LYZ1128 of the present application has good antibacterial activity against 11 pathogens of 6 common alfalfa diseases and Verticillium dahliae (isolated from Oxytropis flavescentis plants), which is a different species from the same genus as Verticillium alfalfa. Potted plant experiments have shown that Bacillus subtilis LYZ1128 can promote the growth of alfalfa roots and plants, and can also significantly reduce the incidence of alfalfa Verticillium wilt. It has a good control effect on alfalfa Verticillium wilt, providing an important theoretical basis for the biological control of alfalfa Verticillium wilt.

The invention relates to the technical field of fertilizers, discloses a microbial agent synergistic type organic-inorganic compound fertilizer special for saline-alkali tolerant alfalfa and a preparation method of the microbial agent synergistic type organic-inorganic compound fertilizer, and aims at solving the problems that germination of alfalfa seeds in saline-alkali soil is blocked, the emergence rate is low and the utilization rate of soil nutrients is insufficient. The organic-inorganic compound fertilizer comprises inorganic components, organic components, compound medium trace elements and compound bacterial powder, the seed dressing microbial inoculum is prepared from paenibacillus polymyxa, sphingomonas and bacillus cereus according to a proportion, and during preparation, the granular organic-inorganic compound fertilizer is prepared through batching, granulation, drying and coating; according to the method, the synergistic effect of the fungicide and the fertilizer is achieved, the emergence rate and the seedling protection rate of alfalfa are increased, plant growth is promoted, meanwhile, the soil structure of the saline-alkali soil is improved, nutrients are activated, the salt resistance and stress resistance of alfalfa are enhanced, and planting benefits are guaranteed.

This invention discloses a novel seedling substrate using plant root stubble as an AM fungal inoculant, its preparation method, and its application. The seedling substrate comprises plant root stubble; the plant root stubble is plant root system infected by arbuscular mycorrhizal fungi (AM fungi); the plant root stubble is derived from alfalfa and plantain. This invention is the first to directly use AM fungal-infected plant root stubble as an AM fungal inoculant in a seedling substrate. This seedling substrate is suitable for seedling planting projects in the field of ecological restoration. It can mainly increase the mycorrhizal fungal infection rate of plants, regulate plant root growth, promote nutrient absorption, enhance plant drought resistance, and enhance plant resistance to pathogens. It plays a particularly important role in seedling planting applications in ecological restoration projects with difficult site conditions.

The present application belongs to the field of grass planting, and particularly relates to a microbial coating agent for relieving salt and alkali stress of alfalfa, a preparation method and application thereof. The microbial coating agent comprises endomycorrhizal fungus powder, Trichoderma harzianum powder, talcum powder, diatomite and carboxymethyl cellulose sodium aqueous solution. The endomycorrhizal fungus and Trichoderma harzianum are combined to coat alfalfa seeds, with Trichoderma harzianum as the main component and endomycorrhizal fungus as the auxiliary component. A small amount of endomycorrhizal fungus and Trichoderma harzianum are used to coat alfalfa seeds, which can relieve salt and alkali stress, significantly improve the emergence rate, promote the increase of alfalfa plant height, root length and biomass, promote the growth of alfalfa, and improve the stress resistance. The microbial coating agent has few components, low fungus content and low cost, and is suitable for large-scale popularization and use.

The invention discloses an alfalfa drought-resistant planting method which comprises the following steps: step 1, selecting a land parcel which is flat in terrain, sufficient in illumination and good in drainage, firstly removing sundries such as broken stones and weeds in the land parcel, then adopting a deep scarification technology to break a plow pan, enabling the deep scarification depth to be 35-45cm, deepening a plow layer, then performing deep ploughing, and crushing massive soil to enable soil particles to be uniform; 2, 8-10 days before sowing, a non-selective herbicide is used for ground treatment, and 100-120 mL of the non-selective herbicide is used per mu to control weed growth; during seed treatment, a biopesticide is adopted for seed dressing; 3, after the land is prepared, the improved compound fertilizer is evenly scattered on the surface of the soil, shallow ploughing is conducted, and the fertilizer and the soil are fully and evenly mixed. The invention belongs to the technical field of crop planting, particularly relates to a drought-resistant planting method of alfalfa, overcomes the defects of drought-resistant planting of the alfalfa in the prior art, and provides the drought-resistant planting method of the alfalfa.

The invention discloses application of a gene MtPrx38 in regulation and control of growth, development and quality of forage grass. The method comprises the following steps: carrying out genome PCR (Polymerase Chain Reaction) identification to obtain homozygotes Prx38-1 and Prx38-2 of the medicago truncatula MtPrx38 gene mutant; rT-PCR (reverse transcription-polymerase chain reaction) detection shows that the expression quantity of the MtPrx38 gene is obviously reduced, and compared with wild medicago truncatula, the peroxidase activity of the mutant homozygotes Prx38-1 and Prx38-2 is obviously reduced. Compared with wild medicago truncatula, the MtPrx38 gene mutant has the advantages that the root length, the lateral root number, the plant height, the branch number, the leaf area and the secondary branch number are obviously increased; the flowering time is advanced; the lignin content and the cellulose content are obviously reduced, which indicates that the gene MtPrx38 has the functions of regulating and controlling the growth, development and quality of forage grass. The method has application prospects in the aspects of promoting growth and development of forage grass, improving forage grass quality and the like.

The invention discloses a medicago sativa MsSANT gene and application of the medicago sativa MsSANT gene in regulation and control of plant height and biological yield. It is found for the first time that overexpressed MsSANT gene can improve the plant height and the biological yield of medicago sativa, and the method has important guiding significance on cultivation of high-yield and high-quality medicago sativa varieties; meanwhile, the molecular mechanism of the MsSANT gene in regulating and controlling the alfalfa plant height is defined, a molecular module for regulating and controlling the alfalfa plant height and the biological yield is further analyzed, the corresponding molecular mechanism can be enriched, and then a new breeding direction is provided for breeding high-yield alfalfa varieties.

The application discloses a method for rapidly detecting alfalfa chromosome ploidy by using flow cytometry, and belongs to the technical field of plant ploidy analysis, and the method comprises the following steps: taking fresh alfalfa leaves, placing the alfalfa leaves, LB01 dissociation solution and steel balls in a container, and using a ball mill to break the tissues at a high frequency to release cell nuclei; then filtering and centrifuging the mixed solution to obtain cell nucleus precipitates; adding pre-cooled dissociation solution and PI staining solution to the precipitates, mixing, and then staining under low-temperature and light-proof conditions, finally preparing stained cell nucleus suspension, and using a flow cytometer to detect, and determining the chromosome ploidy according to the peak value distribution of the fluorescence signal. The application realizes efficient and uniform release and purification of the cell nuclei by using mechanical grinding combined with LB01 dissociation solution, significantly improves the detection efficiency and the atlas quality, has the advantages of simple operation, small sample requirement, high throughput, accurate and reliable results, and is suitable for large-scale rapid identification of different ploidy of alfalfa plants.

This invention discloses a type of alfalfa MsCYP Genes and their applications in salt and cold tolerance. This invention, based on phenotypic and genome association analysis, screened a gene in alfalfa that responds to salt and cold stress. MsCYP The MsCYP The nucleotide sequence is shown in SEQ ID No. 1, and the amino acid sequence is shown in SEQ ID No. 2. Further overexpression in alfalfa hairy roots... MsCYP The results showed that under salt stress or a low temperature of 4°C, overexpression MsCYP The hairy roots of the gene are longer than those of the wild type, and the plant exhibits greater tolerance to salt and low-temperature stress than the wild type, indicating that the gene is overexpressed. MsCYP MsCYP MsCYP MsCYP MsCYP MsCYP MsCYP MsC Genes can enhance the salt and cold tolerance of alfalfa. This invention provides theoretical and practical support for the breeding and widespread promotion of salt- and cold-tolerant alfalfa varieties.

The invention relates to the technical field of grassland restoration, in particular to a degraded grassland restoration microbial agent, a granular restoration microbial agent, a modifier and application of the degraded grassland restoration microbial agent and the modifier. The degraded grassland repairing microbial inoculum provided by the invention is prepared from pseudomonas fluorescens T6 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.36876 and sinorhizobium meliloti NFB5 with the preservation number of CGMCC No.33618. The compounding of the pseudomonas fluorescens T6 and the sinorhizobium meliloti NFB5 in the degraded grassland repairing microbial agent provided by the invention has a synergistic effect, and has a good repairing effect on the degraded grassland and a high strain colonization rate. The method depends on local resources (sheep manure, peat soil, native microorganisms and native grass seeds) of the Inner Mongolia grassland, gives consideration to ecological restoration and animal husbandry production, realizes soil-microorganism-vegetation three-dimensional synergistic integrated restoration, and has important technical innovation value and practical application significance.