62 results about "Medicago" patented technology

The invention relates to the technical field of saline-alkali soil treatment, and discloses a light and moderate saline-alkali soil alfalfa seedling protection and strong growth promotion planting method which comprises the following steps: S1, soil pretreatment and root zone microenvironment construction; s2, seed treatment and space-time double-salt-avoiding sowing; s3, microbial inoculum synergistic application; s4, water and fertilizer integration and accurate management; s5, dynamic regulation and control in the growth period, a low-salt root zone microenvironment is constructed through combination of physical obstruction and biological improvement, salt ascending is inhibited by using a slag salt isolation layer, fertility is improved by using an organic layer, and stress resistance is enhanced by adopting a salt-tolerant variety, climate window sowing and aerogel embedding fungicide; precise regulation and control are achieved through water and fertilizer integrated drip irrigation, the stability of a root zone is maintained by combining intertillage and directional topdressing, finally, the seedling emergence uniformity and the seedling stage survival rate are remarkably improved, and robust growth of plants in saline-alkali soil is promoted.

The invention relates to the field of biological agriculture, in particular to application of a gene MtUMAMIT8 to regulation and control of growth, development and quality of forage grass. The biological function of the gene MtUMAMIT8 is identified for the first time, the MtUMAMIT8 gene is screened and locked through multi-omics analysis, the phenotype of a homozygous mutant NF8465 / NF12630 is analyzed, it is proved that the growth of medicago truncatula is hindered but the lignin content is reduced due to down-regulation of the gene MtUMAMIT8, it is clear that the gene MtUMAMIT8 positively regulates the growth and development of medicago truncatula plant height, the branch number, the leaf size and the like and negatively regulates lignin synthesis, and the gene MtUMAMIT8 can be used for identifying the lignin content of the medicago truncatula. And a new gene resource is provided for regulating and controlling the growth, development and quality of forage grass and breeding forage grass varieties with low lignin content.

The application discloses a sequence of Medicago truncatula AP2 / ERF transcription factor ERM1 and application of the sequence in improving mycorrhizal colonization efficiency of leguminous plants and promoting mycorrhizal symbiosis, and relates to the technical field of plant genetic engineering. ERM1 The gene has a significant promoting effect on the symbiosis of alfalfa and arbuscular mycorrhizal fungi, and the application has important significance for clarifying the biological function of ERM1 in the symbiosis of leguminous plants and mycorrhizal fungi, thereby regulating the symbiosis between plants and mycorrhizal fungi, and provides a research foundation and scientific basis for improving the symbiosis efficiency of leguminous crops by using biological strengthening means.

The application provides a kind of alfalfa population three-dimensional structure phenotype information extraction method and system. First, according to the ground and wall plane in the original point cloud, the original point cloud is corrected in coordinate;Then according to the reason and characteristics of noise generated by original point cloud, based on statistical filtering removes outlier, based on crown voxel filtering removes redundant point, based on moving least square method removes mixed point;Then according to the characteristics of planting and growth of potted alfalfa population, the alfalfa population is extracted by using straight-through filtering and grid division method, and the single plant is segmented, and then six kinds of structure parameters such as maximum plant height, average plant height, plant height quantile, canopy cover, projected leaf area and three-dimensional volume are extracted.The application realizes the lossless automatic extraction of alfalfa population structure phenotype parameters, and the application is also suitable for three-dimensional structure phenotype information extraction of other population growth plants.

This invention discloses a rapid establishment and high-frequency harvesting method for alfalfa cultivation on desert edges without the need for windbreaks, belonging to the field of sandy land ecological agriculture and forage cultivation technology. This method is suitable for shifting sandy areas on desert edges, eliminating the need for windbreaks. Early-maturing forage oats are sown in single rows in spring. After the oats reach a height of 10-20cm and form a windbreak barrier, drought- and salt-tolerant alfalfa is sown in rows between the rows. Drip or sprinkler irrigation is used, and nitrogen, phosphorus, and potassium fertilizers are reduced by 20%-30% compared to conventional methods. Three to five days after each alfalfa harvest, a nutrient solution containing enzymatically hydrolyzed animal-derived small molecule peptides with a molecular weight <1000Da is applied with irrigation water. The oats are harvested in one go after maturity; the alfalfa is harvested frequently when the plants reach a height of 50-60cm, with 5-6 harvests per year. This invention utilizes oats for temporary wind protection, resulting in a lower than 15% mortality rate from wind and sand during the alfalfa seedling stage, enabling planting in the same year. The hay has a crude protein content of 19%–22%, a yield of over 1200 kg of hay per mu, and can be harvested continuously for 8–10 years. It is suitable for desert edge areas with annual precipitation <100 mm.

The application discloses a method for improving productivity of alfalfa grassland in semi-arid area, which comprises the following steps: sowing in the same period strip sowing mode of planting one row of alfalfa every two rows of feed oat; harvesting mixed forage of alfalfa and feed oat only once in the first year, and not ploughing the land after mowing; in the second year, sowing two rows of feed oat in the alfalfa row without ploughing from late April to early May after the alfalfa turns green; harvesting mixed forage of alfalfa and feed oat in the first harvest in the second year; harvesting alfalfa grass in the second and third harvests; in the third year and the following years, sowing two rows of feed oat in the alfalfa row without ploughing after the alfalfa turns green from late April to early May; in the third year and the following years, harvesting mixed forage of alfalfa and feed oat in the first harvest at the initial flowering period of alfalfa in late June; and harvesting alfalfa grass in the second and third harvests. The application has high utilization rate of natural precipitation, does not need irrigation, has high yield, and can effectively improve the productivity of alfalfa grassland in semi-arid area.

A device for improving the germination rate of alfalfa under continuous cropping, relating to the field of seedling technology; comprising a shell, with seedling trays fixedly installed on the left and right sides of the shell from bottom to top, and a feed cylinder assembly that can move up, down, forward, and backward between the seedling trays inside the shell; the feed cylinder assembly includes a funnel-shaped feed cylinder, with an openable and closable cylinder cover at the top of the feed cylinder, and a rotatable rotating cylinder sleeved on the lower outer side of the feed cylinder, with a horizontal feed guide pipe integrally connected to the bottom of the rotating cylinder, and a feed dispensing through hole at the bottom of the feed guide pipe; a peeling cylinder is rotatably connected between the two ends of the feed guide cylinder, with a gap between the outer side of the peeling cylinder and the inner side of the feed guide cylinder, and a shaft connected between the two ends of the peeling cylinder, with opposing spiral blades connected on both sides of the shaft located at the inner center of the peeling cylinder, an axial discharge port on the outer side of the peeling cylinder, and uniformly distributed protrusions integrally connected to the outer side of the peeling cylinder; and an air conditioner installed on the outer side of the shell.

The invention discloses a cysteine-rich small peptide CYSTM5 gene of medicago truncatula and application of the cysteine-rich small peptide CYSTM5 gene, and provides a method for cultivating a cold-resistant and disease-resistant plant by using the MtCYSTM5 gene, and after the gene is over-expressed in medicago truncatula, the cold resistance and disease resistance of transgenic medicago truncatula are improved; meanwhile, after the gene is inserted into a mutant in medicago truncatula through Tnt1, the gene loses efficacy, and the cold resistance and disease resistance of transgenic medicago truncatula are reduced; and after a mutant plant is infected by a complementary mutant carrier, the cold resistance and disease resistance of the mutant can be recovered. The gene for regulating and controlling cold resistance and disease resistance of medicago truncatula is excavated, and a target gene is provided for cold-resistant and disease-resistant molecular breeding of other pasture or crops.

The application focuses on the field of agricultural informatization and remote sensing application technology, and particularly relates to a kind of alfalfa yield estimation method based on multispectral and physical constraint sample enhancement. The method first acquires unmanned aerial vehicle multispectral image data corresponding to the alfalfa growth period, and lays out field quadrats with equal area in the image coverage area, collects measured data of alfalfa hay yield, leaf area index, chlorophyll content and leaf equivalent water thickness, and constructs an initial sample set. Then, input each parameter data within a reasonable range, generate predicted multispectral samples that meet the physical consistency constraint using the radiation transfer model, to expand the sample space. The measured samples and predicted samples are fused to construct an alfalfa hay yield estimation training data set. Based on the training data set, a mapping model between unmanned aerial vehicle multispectral features and alfalfa hay yield is established, and the alfalfa hay yield is estimated through machine learning.

The invention discloses an application of a CKX gene in regulating alfalfa root systems, comprising the following steps: step 1, preparing a transgenic wild-type alfalfa, overexpressing the MtCKX1 gene in the alfalfa, and obtaining a transgenic plant; the cDNA sequence and amino acid sequence of the MtCKX1 gene are sequence 1 and sequence 2 in the sequence table; step 2, comparing the root systems of the transgenic alfalfa plant with those of the wild-type plant, and the root systems of the transgenic alfalfa plant are larger than those of the wild-type plant; the cytokinin content of the transgenic plant is suppressed, and the root systems of the transgenic plant are strengthened and enlarged; cytokinin has a negative regulatory effect on the root growth of the plant, and after the MtCKX1 gene is overexpressed, the cytokinin is suppressed, and the root system of the plant is strengthened and enlarged, thereby effectively fixing the soil and preventing soil loss.

This invention relates to a method for creating late-flowering, high-yielding alfalfa, characterized by the following steps: S1: designing and synthesizing targeted alfalfa MvFT S1: Construct the gRNA of the gene; S2: Construct a CRISPR / Cas9 gene knockout vector containing the gRNA expression cassette described in S1; S3: Transform the knockout vector described in S2 into Agrobacterium tumefaciens; S4: Transform explants of alfalfa cultivars into Agrobacterium tumefaciens as described in S3; S5: Screen, differentiate, and regenerate the explants transformed in S4 to obtain transgenic plants; S6: Perform molecular and phenotypic identification on the transgenic plants to screen for... MvFT A late-flowering, high-yielding germplasm with homozygous gene knockout and significantly delayed flowering time. This invention reveals for the first time... MvFT The key functions of genes in the regulation of flowering in alfalfa were investigated, and new late-flowering, high-yielding alfalfa germplasm was successfully created, providing valuable germplasm resources for breeding new high-yielding and high-quality alfalfa varieties.

The invention relates to the technical field of fertilizers, discloses a microbial agent synergistic type organic-inorganic compound fertilizer special for saline-alkali tolerant alfalfa and a preparation method of the microbial agent synergistic type organic-inorganic compound fertilizer, and aims at solving the problems that germination of alfalfa seeds in saline-alkali soil is blocked, the emergence rate is low and the utilization rate of soil nutrients is insufficient. The organic-inorganic compound fertilizer comprises inorganic components, organic components, compound medium trace elements and compound bacterial powder, the seed dressing microbial inoculum is prepared from paenibacillus polymyxa, sphingomonas and bacillus cereus according to a proportion, and during preparation, the granular organic-inorganic compound fertilizer is prepared through batching, granulation, drying and coating; according to the method, the synergistic effect of the fungicide and the fertilizer is achieved, the emergence rate and the seedling protection rate of alfalfa are increased, plant growth is promoted, meanwhile, the soil structure of the saline-alkali soil is improved, nutrients are activated, the salt resistance and stress resistance of alfalfa are enhanced, and planting benefits are guaranteed.

This invention discloses a novel seedling substrate using plant root stubble as an AM fungal inoculant, its preparation method, and its application. The seedling substrate comprises plant root stubble; the plant root stubble is plant root system infected by arbuscular mycorrhizal fungi (AM fungi); the plant root stubble is derived from alfalfa and plantain. This invention is the first to directly use AM fungal-infected plant root stubble as an AM fungal inoculant in a seedling substrate. This seedling substrate is suitable for seedling planting projects in the field of ecological restoration. It can mainly increase the mycorrhizal fungal infection rate of plants, regulate plant root growth, promote nutrient absorption, enhance plant drought resistance, and enhance plant resistance to pathogens. It plays a particularly important role in seedling planting applications in ecological restoration projects with difficult site conditions.

The present application belongs to the field of grass planting, and particularly relates to a microbial coating agent for relieving salt and alkali stress of alfalfa, a preparation method and application thereof. The microbial coating agent comprises endomycorrhizal fungus powder, Trichoderma harzianum powder, talcum powder, diatomite and carboxymethyl cellulose sodium aqueous solution. The endomycorrhizal fungus and Trichoderma harzianum are combined to coat alfalfa seeds, with Trichoderma harzianum as the main component and endomycorrhizal fungus as the auxiliary component. A small amount of endomycorrhizal fungus and Trichoderma harzianum are used to coat alfalfa seeds, which can relieve salt and alkali stress, significantly improve the emergence rate, promote the increase of alfalfa plant height, root length and biomass, promote the growth of alfalfa, and improve the stress resistance. The microbial coating agent has few components, low fungus content and low cost, and is suitable for large-scale popularization and use.

The invention discloses an alfalfa drought-resistant planting method which comprises the following steps: step 1, selecting a land parcel which is flat in terrain, sufficient in illumination and good in drainage, firstly removing sundries such as broken stones and weeds in the land parcel, then adopting a deep scarification technology to break a plow pan, enabling the deep scarification depth to be 35-45cm, deepening a plow layer, then performing deep ploughing, and crushing massive soil to enable soil particles to be uniform; 2, 8-10 days before sowing, a non-selective herbicide is used for ground treatment, and 100-120 mL of the non-selective herbicide is used per mu to control weed growth; during seed treatment, a biopesticide is adopted for seed dressing; 3, after the land is prepared, the improved compound fertilizer is evenly scattered on the surface of the soil, shallow ploughing is conducted, and the fertilizer and the soil are fully and evenly mixed. The invention belongs to the technical field of crop planting, particularly relates to a drought-resistant planting method of alfalfa, overcomes the defects of drought-resistant planting of the alfalfa in the prior art, and provides the drought-resistant planting method of the alfalfa.

The invention discloses application of a gene MtPrx38 in regulation and control of growth, development and quality of forage grass. The method comprises the following steps: carrying out genome PCR (Polymerase Chain Reaction) identification to obtain homozygotes Prx38-1 and Prx38-2 of the medicago truncatula MtPrx38 gene mutant; rT-PCR (reverse transcription-polymerase chain reaction) detection shows that the expression quantity of the MtPrx38 gene is obviously reduced, and compared with wild medicago truncatula, the peroxidase activity of the mutant homozygotes Prx38-1 and Prx38-2 is obviously reduced. Compared with wild medicago truncatula, the MtPrx38 gene mutant has the advantages that the root length, the lateral root number, the plant height, the branch number, the leaf area and the secondary branch number are obviously increased; the flowering time is advanced; the lignin content and the cellulose content are obviously reduced, which indicates that the gene MtPrx38 has the functions of regulating and controlling the growth, development and quality of forage grass. The method has application prospects in the aspects of promoting growth and development of forage grass, improving forage grass quality and the like.

The invention discloses a medicago sativa MsSANT gene and application of the medicago sativa MsSANT gene in regulation and control of plant height and biological yield. It is found for the first time that overexpressed MsSANT gene can improve the plant height and the biological yield of medicago sativa, and the method has important guiding significance on cultivation of high-yield and high-quality medicago sativa varieties; meanwhile, the molecular mechanism of the MsSANT gene in regulating and controlling the alfalfa plant height is defined, a molecular module for regulating and controlling the alfalfa plant height and the biological yield is further analyzed, the corresponding molecular mechanism can be enriched, and then a new breeding direction is provided for breeding high-yield alfalfa varieties.

The application discloses a method for rapidly detecting alfalfa chromosome ploidy by using flow cytometry, and belongs to the technical field of plant ploidy analysis, and the method comprises the following steps: taking fresh alfalfa leaves, placing the alfalfa leaves, LB01 dissociation solution and steel balls in a container, and using a ball mill to break the tissues at a high frequency to release cell nuclei; then filtering and centrifuging the mixed solution to obtain cell nucleus precipitates; adding pre-cooled dissociation solution and PI staining solution to the precipitates, mixing, and then staining under low-temperature and light-proof conditions, finally preparing stained cell nucleus suspension, and using a flow cytometer to detect, and determining the chromosome ploidy according to the peak value distribution of the fluorescence signal. The application realizes efficient and uniform release and purification of the cell nuclei by using mechanical grinding combined with LB01 dissociation solution, significantly improves the detection efficiency and the atlas quality, has the advantages of simple operation, small sample requirement, high throughput, accurate and reliable results, and is suitable for large-scale rapid identification of different ploidy of alfalfa plants.

This invention discloses a type of alfalfa MsCYP Genes and their applications in salt and cold tolerance. This invention, based on phenotypic and genome association analysis, screened a gene in alfalfa that responds to salt and cold stress. MsCYP The MsCYP The nucleotide sequence is shown in SEQ ID No. 1, and the amino acid sequence is shown in SEQ ID No. 2. Further overexpression in alfalfa hairy roots... MsCYP The results showed that under salt stress or a low temperature of 4°C, overexpression MsCYP The hairy roots of the gene are longer than those of the wild type, and the plant exhibits greater tolerance to salt and low-temperature stress than the wild type, indicating that the gene is overexpressed. MsCYP MsCYP MsCYP MsCYP MsCYP MsCYP MsCYP MsC Genes can enhance the salt and cold tolerance of alfalfa. This invention provides theoretical and practical support for the breeding and widespread promotion of salt- and cold-tolerant alfalfa varieties.

The invention relates to the technical field of grassland restoration, in particular to a degraded grassland restoration microbial agent, a granular restoration microbial agent, a modifier and application of the degraded grassland restoration microbial agent and the modifier. The degraded grassland repairing microbial inoculum provided by the invention is prepared from pseudomonas fluorescens T6 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.36876 and sinorhizobium meliloti NFB5 with the preservation number of CGMCC No.33618. The compounding of the pseudomonas fluorescens T6 and the sinorhizobium meliloti NFB5 in the degraded grassland repairing microbial agent provided by the invention has a synergistic effect, and has a good repairing effect on the degraded grassland and a high strain colonization rate. The method depends on local resources (sheep manure, peat soil, native microorganisms and native grass seeds) of the Inner Mongolia grassland, gives consideration to ecological restoration and animal husbandry production, realizes soil-microorganism-vegetation three-dimensional synergistic integrated restoration, and has important technical innovation value and practical application significance.

The application discloses a synthetic bacterial flora capable of improving salt tolerance of alfalfa, which is composed of Pseudomonas strain EC5, Agrobacterium strain EC17, Streptomyces strain EC15, Paracoccus strain RH39, Pseudomonas strain RH6, Pseudomonas strain RH37, Sporosarcina strain RH34 and Bacillus strain RH32. The synthetic bacterial flora has the functions of dissolving organic phosphorus, dissolving inorganic phosphorus, dissolving potassium, producing siderophore, producing biofilm, producing IAA and producing ACC deaminase. Seed soaking and germination under the stress of 200 mmol / L and 250 mmol / L NaCl can obviously improve the germination potential, germination rate, germination index, radicle length, plumule length and fresh weight of the group seedling embryo of alfalfa. Under the stress of 200 mmol / L NaCl, seed soaking with the synthetic bacterial flora can significantly improve the plant height, stem diameter, leaf area and fresh and dry weight of the above-ground part of alfalfa.

An alfalfa planting liquid fertilizer sprinkling irrigation device belongs to the technical field of sprinkling irrigation devices. The device adopts a double-cavity storage tank design, realizes switching irrigation of liquid fertilizer and irrigation water through a movable sealing plate, and solves the problems of single function of traditional equipment and inability to adjust the irrigation strategy in time. The device comprises a foldable sprinkling irrigation arm assembly, realizes angle adjustment of an irrigation pipe through a cam mechanism to adapt to a sloping land, sets a shared liquid discharge tank at the bottom of double bins, controls opening and closing of a discharge pipe of a corresponding bin chamber through displacement control of the sealing plate, cooperates with a magnetic suction type transmission mechanism to drive rotation of a stirring shaft to prevent liquid deposition, adopts a movable frame power-driven transmission system to realize linkage of liquid switching and stirring functions, additionally sets a buoyancy-driven camera turning mechanism, makes a floating plate drive a lifting rod to move through thrust of a stirring blade and buoyancy of liquid, and realizes expansion of a monitoring viewing angle. The device is used for on-demand sprinkling irrigation of liquid fertilizer and irrigation water in alfalfa planting, realizes integrated functions of irrigation mode switching, deposition prevention and growth monitoring.

The present application relates to the field of biological agriculture, and in particular to the application of the gene MtUMAMIT8 in regulating the growth and development and quality of forage grass. The biological function of the gene MtUMAMIT8 is identified for the first time, the MtUMAMIT8 gene is screened and locked through multi-omics analysis, and the phenotype of the homozygous mutant NF8465 / NF12630 is analyzed, confirming that the down-regulation of the gene MtUMAMIT8 leads to the growth of Medicago truncatula being hindered but the lignin content being reduced, and that the gene MtUMAMIT8 positively regulates the growth and development of the plant height, branch number and leaf size of Medicago truncatula and negatively regulates the synthesis of lignin, thereby providing a new gene resource for regulating the growth and development and quality of forage grass and breeding forage grass varieties with low lignin content.

This invention discloses a microbial remediation method for improving the soil quality of desert grasslands. The method involves sowing grass seeds and applying microbial fertilizer to pre-treated plots. The grass seed rate is 1-5 kg ​​per mu (approximately 0.067 hectares), and the fertilizer application rate is 15-45 kg per mu. The sowing row spacing is 35 cm, and the sowing depth is 2-3 cm. The grass seeds, by weight, include 40-60 parts of *Gnaphalium affine*, 5-15 parts of *Alternanthera philoxeroides*, and 30-50 parts of *Isodon japonicus*. The microbial fertilizer includes nitrogen-fixing bacteria, phosphate-solubilizing bacteria, and growth-promoting bacteria, with an organic matter content ≥40% and a pH value of 6.5-7.5. Specifically, the nitrogen-fixing bacteria in the microbial fertilizer are *Rhizobium alfalfa* or *Rhizobium soybean*; the phosphate-solubilizing bacteria are *Pseudomonas fluorescens* or *Pseudomonas putida*; and the growth-promoting bacteria are *Bacillus subtilis* or *Bacillus licheniformis*. The weight ratio of the nitrogen-fixing bacteria, phosphate-solubilizing bacteria, and growth-promoting bacteria is 2:1-1.5:1-1.5. This invention effectively improves the physical, chemical, and microbial properties of soil by applying microbial fertilizers and sowing grass seeds.

Recombinant DNA molecules and constructs useful for modulating gene expression in plants, including molecules derived from Medicago truncatula sequences, are provided. Plants, plant cells, plant parts, and seeds comprising recombinant DNA molecules operably linked to heterologous transcribable DNA molecules are further provided, as are methods of their use.

The present invention discloses a method for producing chimeric silk using silkworms by using Autographa californica nuclear polyhedrosis virus, and constructs a chimeric silk containing FibL-MaSp-c-polyA FibL The plasmid pFAST‑FibL‑MaSp‑c of the fragment is transformed into Escherichia coli containing AcBacmid DH10Ac, and recombinant AcBacmid‑FibL‑MaSp‑c is obtained by screening. The recombinant virus AcNPV‑FibL‑MaSp‑c is transfected into Sf‑9 cells to obtain the recombinant virus AcNPV‑FibL‑MaSp‑c, which is inoculated into 5-instar silkworm larvae, and the larvae are fed with cool-dried fresh mulberry leaves soaked or sprayed with antibiotic liquid for one day, and then fed with fresh mulberry leaves until they mature. The mature silkworms are fed with fresh mulberry leaves soaked or sprayed with ecdysteroid liquid; or the mature silkworms are directly sprayed with ecdysteroid liquid, and the mature silkworms are moved to a cocooning machine, cocoons are harvested after cocooning, and the cocoons are dried and reeled to obtain chimeric silk, thereby meeting the demand for diversity of silk protein in the preparation of various biomaterials.

The invention discloses an alfalfa optimal harvesting period identification method, system and device and a medium, and relates to the technical field of intelligent agriculture, and the method comprises the steps: collecting RGB image data of alfalfa flowers; performing multi-scale feature extraction on the alfalfa flower RGB image data, representing biaxial pooling operation and normalization operation on each scale feature, generating pooling features and normalization features, and calculating attention to obtain feature representation of each scale feature after enhancement; calculating an initial fusion feature channel enhancement feature; independently adding a spatial attention mechanism to each scale feature representation, and performing spatial weight association on the channel enhancement feature and each scale feature representation to generate a spatial calibration feature; fusing each space calibration feature, outputting a final feature, and predicting the number of flowers; when the flowering rate reaches a preset threshold value, the harvesting period is determined to be the optimal harvesting period; according to the method, daily high-precision monitoring can be realized, and the dynamic change process of the alfalfa flowering rate can be accurately captured.

The invention discloses alfalfa plant type and biomass related genes MtFVEa and MtFVEb as well as an encoding protein and application thereof. According to the invention, two highly homologous MtFVEa and MtFVEb genes are separated from alfalfa, and the nucleotide sequences of CDS of the MtFVEa and MtFVEb genes are respectively shown as SEQ ID NO. 1 or SEQ ID NO. 4. Genetic transformation experiments find that MtFVEa or MtFVEb genes in forage grass crops are mutated or interfered respectively, and the plant height or / and biomass of the crops can be remarkably reduced by reducing the expression quantity or expression level of the MtFVEa or MtFVEb genes in the forage grass crops; meanwhile, the expression quantity or the expression level of the MtFVEa gene and the MtFVEb gene is reduced, and the plant height or / and the biomass of forage grass crops is more remarkably reduced. The invention has application potential in the aspects of regulating and controlling the yield and quality of forage grass crops or cultivating short forage grass crop varieties and the like.

The application discloses a wild flower combination for inhibiting farmland pest earwigs and a planting and management method thereof, and belongs to the technical field of ecological control of agricultural pests. The wild flower combination for inhibiting farmland pest earwigs comprises the following flower species: red clover, zanthaxanthin, coriander, licorice, forget-me-not, purple flowered ground ivy, yarrow, mint, alfalfa and flos malvae. The application creates a rich flower environment through a wild flower buffer zone, creates a specific high-density vegetation area through the fragrance and potential allelopathy of the flowers, influences the avoidance effect of the earwig population, limits the activity range of the earwigs, fundamentally changes the ecological environment of the area, and effectively regulates the earwig population density.

The invention discloses a flavonoid compound, a composition and application of the flavonoid compound to prevention and treatment of plant pests, the structure of the flavonoid compound is shown in a formula I, and the flavonoid compound can be used for preventing pests with piercing-sucking mouthparts and pests with rasping-sucking mouthparts, especially pests caused by Amyzus pisum and Odontothrips loti to medicago sativa 'Ladak +', and the flavonoid compound can be used for preventing pests caused by Amyzus pisum and Odontothrips loti to medicago sativa 'Ladak +'. The flavonoid compound disclosed by the invention is derived from a plant secondary metabolite, has the advantages of good environmental compatibility, easiness in degradation, relatively strong target specificity and the like, and accords with the development direction of green pesticides. Therefore, the invention not only provides important material basis and application basis for the development of efficient and safe botanical insecticides, but also has positive significance for promoting the production and ecological sustainable development of the grass industry.