41 results about "Medicago" patented technology

The application discloses a sequence of Medicago truncatula AP2 / ERF transcription factor ERM1 and application of the sequence in improving mycorrhizal colonization efficiency of leguminous plants and promoting mycorrhizal symbiosis, and relates to the technical field of plant genetic engineering. ERM1 The gene has a significant promoting effect on the symbiosis of alfalfa and arbuscular mycorrhizal fungi, and the application has important significance for clarifying the biological function of ERM1 in the symbiosis of leguminous plants and mycorrhizal fungi, thereby regulating the symbiosis between plants and mycorrhizal fungi, and provides a research foundation and scientific basis for improving the symbiosis efficiency of leguminous crops by using biological strengthening means.

The application provides a kind of alfalfa population three-dimensional structure phenotype information extraction method and system. First, according to the ground and wall plane in the original point cloud, the original point cloud is corrected in coordinate;Then according to the reason and characteristics of noise generated by original point cloud, based on statistical filtering removes outlier, based on crown voxel filtering removes redundant point, based on moving least square method removes mixed point;Then according to the characteristics of planting and growth of potted alfalfa population, the alfalfa population is extracted by using straight-through filtering and grid division method, and the single plant is segmented, and then six kinds of structure parameters such as maximum plant height, average plant height, plant height quantile, canopy cover, projected leaf area and three-dimensional volume are extracted.The application realizes the lossless automatic extraction of alfalfa population structure phenotype parameters, and the application is also suitable for three-dimensional structure phenotype information extraction of other population growth plants.

A device for improving the germination rate of alfalfa under continuous cropping, relating to the field of seedling technology; comprising a shell, with seedling trays fixedly installed on the left and right sides of the shell from bottom to top, and a feed cylinder assembly that can move up, down, forward, and backward between the seedling trays inside the shell; the feed cylinder assembly includes a funnel-shaped feed cylinder, with an openable and closable cylinder cover at the top of the feed cylinder, and a rotatable rotating cylinder sleeved on the lower outer side of the feed cylinder, with a horizontal feed guide pipe integrally connected to the bottom of the rotating cylinder, and a feed dispensing through hole at the bottom of the feed guide pipe; a peeling cylinder is rotatably connected between the two ends of the feed guide cylinder, with a gap between the outer side of the peeling cylinder and the inner side of the feed guide cylinder, and a shaft connected between the two ends of the peeling cylinder, with opposing spiral blades connected on both sides of the shaft located at the inner center of the peeling cylinder, an axial discharge port on the outer side of the peeling cylinder, and uniformly distributed protrusions integrally connected to the outer side of the peeling cylinder; and an air conditioner installed on the outer side of the shell.

The application focuses on the field of agricultural informatization and remote sensing application technology, and particularly relates to a kind of alfalfa yield estimation method based on multispectral and physical constraint sample enhancement. The method first acquires unmanned aerial vehicle multispectral image data corresponding to the alfalfa growth period, and lays out field quadrats with equal area in the image coverage area, collects measured data of alfalfa hay yield, leaf area index, chlorophyll content and leaf equivalent water thickness, and constructs an initial sample set. Then, input each parameter data within a reasonable range, generate predicted multispectral samples that meet the physical consistency constraint using the radiation transfer model, to expand the sample space. The measured samples and predicted samples are fused to construct an alfalfa hay yield estimation training data set. Based on the training data set, a mapping model between unmanned aerial vehicle multispectral features and alfalfa hay yield is established, and the alfalfa hay yield is estimated through machine learning.

This invention relates to a method for creating late-flowering, high-yielding alfalfa, characterized by the following steps: S1: designing and synthesizing targeted alfalfa MvFT S1: Construct the gRNA of the gene; S2: Construct a CRISPR / Cas9 gene knockout vector containing the gRNA expression cassette described in S1; S3: Transform the knockout vector described in S2 into Agrobacterium tumefaciens; S4: Transform explants of alfalfa cultivars into Agrobacterium tumefaciens as described in S3; S5: Screen, differentiate, and regenerate the explants transformed in S4 to obtain transgenic plants; S6: Perform molecular and phenotypic identification on the transgenic plants to screen for... MvFT A late-flowering, high-yielding germplasm with homozygous gene knockout and significantly delayed flowering time. This invention reveals for the first time... MvFT The key functions of genes in the regulation of flowering in alfalfa were investigated, and new late-flowering, high-yielding alfalfa germplasm was successfully created, providing valuable germplasm resources for breeding new high-yielding and high-quality alfalfa varieties.

The invention relates to the technical field of fertilizers, discloses a microbial agent synergistic type organic-inorganic compound fertilizer special for saline-alkali tolerant alfalfa and a preparation method of the microbial agent synergistic type organic-inorganic compound fertilizer, and aims at solving the problems that germination of alfalfa seeds in saline-alkali soil is blocked, the emergence rate is low and the utilization rate of soil nutrients is insufficient. The organic-inorganic compound fertilizer comprises inorganic components, organic components, compound medium trace elements and compound bacterial powder, the seed dressing microbial inoculum is prepared from paenibacillus polymyxa, sphingomonas and bacillus cereus according to a proportion, and during preparation, the granular organic-inorganic compound fertilizer is prepared through batching, granulation, drying and coating; according to the method, the synergistic effect of the fungicide and the fertilizer is achieved, the emergence rate and the seedling protection rate of alfalfa are increased, plant growth is promoted, meanwhile, the soil structure of the saline-alkali soil is improved, nutrients are activated, the salt resistance and stress resistance of alfalfa are enhanced, and planting benefits are guaranteed.

This invention discloses a novel seedling substrate using plant root stubble as an AM fungal inoculant, its preparation method, and its application. The seedling substrate comprises plant root stubble; the plant root stubble is plant root system infected by arbuscular mycorrhizal fungi (AM fungi); the plant root stubble is derived from alfalfa and plantain. This invention is the first to directly use AM fungal-infected plant root stubble as an AM fungal inoculant in a seedling substrate. This seedling substrate is suitable for seedling planting projects in the field of ecological restoration. It can mainly increase the mycorrhizal fungal infection rate of plants, regulate plant root growth, promote nutrient absorption, enhance plant drought resistance, and enhance plant resistance to pathogens. It plays a particularly important role in seedling planting applications in ecological restoration projects with difficult site conditions.

The present application belongs to the field of grass planting, and particularly relates to a microbial coating agent for relieving salt and alkali stress of alfalfa, a preparation method and application thereof. The microbial coating agent comprises endomycorrhizal fungus powder, Trichoderma harzianum powder, talcum powder, diatomite and carboxymethyl cellulose sodium aqueous solution. The endomycorrhizal fungus and Trichoderma harzianum are combined to coat alfalfa seeds, with Trichoderma harzianum as the main component and endomycorrhizal fungus as the auxiliary component. A small amount of endomycorrhizal fungus and Trichoderma harzianum are used to coat alfalfa seeds, which can relieve salt and alkali stress, significantly improve the emergence rate, promote the increase of alfalfa plant height, root length and biomass, promote the growth of alfalfa, and improve the stress resistance. The microbial coating agent has few components, low fungus content and low cost, and is suitable for large-scale popularization and use.

The application discloses a method for rapidly detecting alfalfa chromosome ploidy by using flow cytometry, and belongs to the technical field of plant ploidy analysis, and the method comprises the following steps: taking fresh alfalfa leaves, placing the alfalfa leaves, LB01 dissociation solution and steel balls in a container, and using a ball mill to break the tissues at a high frequency to release cell nuclei; then filtering and centrifuging the mixed solution to obtain cell nucleus precipitates; adding pre-cooled dissociation solution and PI staining solution to the precipitates, mixing, and then staining under low-temperature and light-proof conditions, finally preparing stained cell nucleus suspension, and using a flow cytometer to detect, and determining the chromosome ploidy according to the peak value distribution of the fluorescence signal. The application realizes efficient and uniform release and purification of the cell nuclei by using mechanical grinding combined with LB01 dissociation solution, significantly improves the detection efficiency and the atlas quality, has the advantages of simple operation, small sample requirement, high throughput, accurate and reliable results, and is suitable for large-scale rapid identification of different ploidy of alfalfa plants.

This invention discloses a type of alfalfa MsCYP Genes and their applications in salt and cold tolerance. This invention, based on phenotypic and genome association analysis, screened a gene in alfalfa that responds to salt and cold stress. MsCYP The MsCYP The nucleotide sequence is shown in SEQ ID No. 1, and the amino acid sequence is shown in SEQ ID No. 2. Further overexpression in alfalfa hairy roots... MsCYP The results showed that under salt stress or a low temperature of 4°C, overexpression MsCYP The hairy roots of the gene are longer than those of the wild type, and the plant exhibits greater tolerance to salt and low-temperature stress than the wild type, indicating that the gene is overexpressed. MsCYP MsCYP MsCYP MsCYP MsCYP MsCYP MsCYP MsC Genes can enhance the salt and cold tolerance of alfalfa. This invention provides theoretical and practical support for the breeding and widespread promotion of salt- and cold-tolerant alfalfa varieties.

The invention relates to the technical field of grassland restoration, in particular to a degraded grassland restoration microbial agent, a granular restoration microbial agent, a modifier and application of the degraded grassland restoration microbial agent and the modifier. The degraded grassland repairing microbial inoculum provided by the invention is prepared from pseudomonas fluorescens T6 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.36876 and sinorhizobium meliloti NFB5 with the preservation number of CGMCC No.33618. The compounding of the pseudomonas fluorescens T6 and the sinorhizobium meliloti NFB5 in the degraded grassland repairing microbial agent provided by the invention has a synergistic effect, and has a good repairing effect on the degraded grassland and a high strain colonization rate. The method depends on local resources (sheep manure, peat soil, native microorganisms and native grass seeds) of the Inner Mongolia grassland, gives consideration to ecological restoration and animal husbandry production, realizes soil-microorganism-vegetation three-dimensional synergistic integrated restoration, and has important technical innovation value and practical application significance.

The application discloses a synthetic bacterial flora capable of improving salt tolerance of alfalfa, which is composed of Pseudomonas strain EC5, Agrobacterium strain EC17, Streptomyces strain EC15, Paracoccus strain RH39, Pseudomonas strain RH6, Pseudomonas strain RH37, Sporosarcina strain RH34 and Bacillus strain RH32. The synthetic bacterial flora has the functions of dissolving organic phosphorus, dissolving inorganic phosphorus, dissolving potassium, producing siderophore, producing biofilm, producing IAA and producing ACC deaminase. Seed soaking and germination under the stress of 200 mmol / L and 250 mmol / L NaCl can obviously improve the germination potential, germination rate, germination index, radicle length, plumule length and fresh weight of the group seedling embryo of alfalfa. Under the stress of 200 mmol / L NaCl, seed soaking with the synthetic bacterial flora can significantly improve the plant height, stem diameter, leaf area and fresh and dry weight of the above-ground part of alfalfa.

An alfalfa planting liquid fertilizer sprinkling irrigation device belongs to the technical field of sprinkling irrigation devices. The device adopts a double-cavity storage tank design, realizes switching irrigation of liquid fertilizer and irrigation water through a movable sealing plate, and solves the problems of single function of traditional equipment and inability to adjust the irrigation strategy in time. The device comprises a foldable sprinkling irrigation arm assembly, realizes angle adjustment of an irrigation pipe through a cam mechanism to adapt to a sloping land, sets a shared liquid discharge tank at the bottom of double bins, controls opening and closing of a discharge pipe of a corresponding bin chamber through displacement control of the sealing plate, cooperates with a magnetic suction type transmission mechanism to drive rotation of a stirring shaft to prevent liquid deposition, adopts a movable frame power-driven transmission system to realize linkage of liquid switching and stirring functions, additionally sets a buoyancy-driven camera turning mechanism, makes a floating plate drive a lifting rod to move through thrust of a stirring blade and buoyancy of liquid, and realizes expansion of a monitoring viewing angle. The device is used for on-demand sprinkling irrigation of liquid fertilizer and irrigation water in alfalfa planting, realizes integrated functions of irrigation mode switching, deposition prevention and growth monitoring.

The present application relates to the field of biological agriculture, and in particular to the application of the gene MtUMAMIT8 in regulating the growth and development and quality of forage grass. The biological function of the gene MtUMAMIT8 is identified for the first time, the MtUMAMIT8 gene is screened and locked through multi-omics analysis, and the phenotype of the homozygous mutant NF8465 / NF12630 is analyzed, confirming that the down-regulation of the gene MtUMAMIT8 leads to the growth of Medicago truncatula being hindered but the lignin content being reduced, and that the gene MtUMAMIT8 positively regulates the growth and development of the plant height, branch number and leaf size of Medicago truncatula and negatively regulates the synthesis of lignin, thereby providing a new gene resource for regulating the growth and development and quality of forage grass and breeding forage grass varieties with low lignin content.

Recombinant DNA molecules and constructs useful for modulating gene expression in plants, including molecules derived from Medicago truncatula sequences, are provided. Plants, plant cells, plant parts, and seeds comprising recombinant DNA molecules operably linked to heterologous transcribable DNA molecules are further provided, as are methods of their use.

The invention discloses a flavonoid compound, a composition and application of the flavonoid compound to prevention and treatment of plant pests, the structure of the flavonoid compound is shown in a formula I, and the flavonoid compound can be used for preventing pests with piercing-sucking mouthparts and pests with rasping-sucking mouthparts, especially pests caused by Amyzus pisum and Odontothrips loti to medicago sativa 'Ladak +', and the flavonoid compound can be used for preventing pests caused by Amyzus pisum and Odontothrips loti to medicago sativa 'Ladak +'. The flavonoid compound disclosed by the invention is derived from a plant secondary metabolite, has the advantages of good environmental compatibility, easiness in degradation, relatively strong target specificity and the like, and accords with the development direction of green pesticides. Therefore, the invention not only provides important material basis and application basis for the development of efficient and safe botanical insecticides, but also has positive significance for promoting the production and ecological sustainable development of the grass industry.

The invention discloses a method for screening a drought-tolerant alfalfa variety, and relates to the technical field of forage grass breeding, and the method comprises the following specific steps: uniformly treating seeds, planting and culturing, carrying out drought stress treatment in a four-leaf stage, collecting a leaf sample in a stress later stage, carrying out liquid nitrogen cryopreservation, and obtaining metabolite data by using a chromatography-mass spectrometry technology, a characteristic metabolite fingerprint spectrum is constructed, drought-resistant alfalfa varieties and materials are screened in combination with a cosine similarity algorithm, a complete technical path from seeds to variety and material identification and screening is formed, and efficient screening and breeding of the drought-resistant varieties are achieved. According to the method, interference is reduced through a standardized process, reliable data is provided in combination with metabonomics analysis, screening scientificity is improved by constructing a characteristic metabolite fingerprint spectrum and a cosine similarity algorithm, result stability is ensured through a preset threshold value and repeated verification, batch screening and variety improvement are supported, the operation threshold is reduced, drought-enduring varieties are accurately recognized, and the method is suitable for large-scale popularization and application. And the alfalfa variety improvement and industrial application are efficiently promoted.

The application provides a kind of based on ginkgo root secretion regulation alfalfa nodulation growth-promoting forest-grass interplanting method.The method makes full use of ginkgo under forest space, by land preparation and fertilization between forest rows, constructs high ridge square, selects high-quality alfalfa seed for strip sowing, combined with reasonable spacing and fixing seedling, cultivation and weeding and other field management measures, realize the normal growth and efficient utilization of alfalfa, provide a scientific and reasonable alfalfa interplanting mode.The application combines soil physical and chemical index determination, systematically evaluates forest soil before and after alfalfa planting, confirms that the method can effectively improve soil physical and chemical properties, promote ginkgo-alfalfa synergistic growth, has significant ecological benefits and application prospect.In addition, the application further verifies that ginkgo root secretion has obvious growth-promoting effect on alfalfa rhizobium, and can improve the expression level of alfalfa nod gene, enhance the number and quality of nodule, so as to improve the production performance of alfalfa and soil improvement effect.

The invention discloses a method for preparing vegetation planting soil by utilizing industrial solid waste and microorganisms and application of the vegetation planting soil. The method comprises the following steps: mixing 80-90% of high titanium slag, 7-15% of sandy soil or phosphogypsum and 2-5% of titanium gypsum into a vegetation matrix according to specific particle size grading; inoculating a composite strain consisting of bacillus mucilaginosus and azotobacter chroococcum, fermenting at 30 DEG C for 12-18 days, and dynamically monitoring the contents of alkali-hydrolyzable nitrogen, available phosphorus and potassium and biologically extractable titanium; then, alfalfa is planted, arbuscular mycorrhizal fungi and rhizobium suspensions with the OD600 being 0.8-1.0 are regularly irrigated, and curing is conducted for 12-18 days; and irrigating and maintaining for 12-18 days by using chlorella propagation liquid every 4-6 days, and finally reserving the whole plant or harvesting to obtain the plant growing planting soil. According to the method, industrial solid waste is efficiently converted into a functional planting medium through the multi-stage biological-mineral synergistic effect, solid waste recycling and soil function reconstruction are achieved, and the method has the environment restoration and ecological reconstruction value.

The present application discloses a strain of Trichoderma suitable for promoting growth and improving competitiveness of alfalfa in degraded grassland, and a bacterial fertilizer product thereof, the strain NAU-HL7 is classified and named as Trichoderma pini (Trichoderma pini) Trichoderma pinicola ), and was preserved in China Center for Type Culture Collection on October 30, 2024, with a strain preservation number of CCTCC NO: M 20242377. The strain NAU-HL7 is used to prepare a bacterial fertilizer product suitable for promoting growth and improving competitiveness of alfalfa in degraded grassland, and the mass ratio of the Trichoderma solid fermentation, calcium superphosphate and controlled-release nitrogen fertilizer in the product is 1:(15-25):(8-12). The product can be used for seedling stage planting and rapid growth of alfalfa in degraded grassland in the north, so as to compete faster than the original grassland vegetation, ensure successful supplemental planting of high-quality forage, overwintering and regreening, and improve grassland productivity.

The invention discloses application of casein kinase in regulation and control of flowering and leaf development of medicago sativa, and belongs to the technical field of biological genetic engineering. The multifunctionality of MsPPK3 as a key regulatory factor in coordination of vegetative growth and reproductive growth balance of medicago sativa is clarified for the first time, and specifically, MsPPK3 positively regulates plant reproductive growth and negatively regulates plant vegetative growth. Wherein the positive regulation and control of the reproductive growth of the plant is embodied in overexpression early flowering and silence late flowering. The negative regulation and control of plant vegetative growth is characterized in that the overexpressed plant is in a fine stem scattered form, and leaves are miniaturized, thinned and / or weakened form; the silent plants present a thick stem cluster type, a large leaf size, a thick leaf and / or a strengthened leaf. The method has great significance in the aspects of cultivation of different new varieties / new strains of alfalfa and the like.

The invention discloses related genes MtTFLa and MtTFL in the mature period of alfalfa pods as well as an encoding protein and application of the related genes MtTFLa and MtTFL. According to the invention, an MtTFLa gene and an MtTFLb gene which are highly homologous are separated from alfalfa, the nucleotide sequence of the CDS of the MtTFLa gene is shown as SEQ ID NO.1, and the nucleotide sequence of the CDS of the MtTFLb gene is shown as SEQ ID NO.4. According to the invention, the MtTFLa gene and the MtTFLb gene which are highly homologous are separated from alfalfa; it is found that MtTFLa or MtTFLb genes in leguminous plants are mutated or interfered, and after the expression quantity or expression level of the MtTFLa or MtTFLb genes in the leguminous plants is reduced, fruit pod maturation of the leguminous plants can be effectively promoted, including fruit pod maturation time tends to be consistent or the number of mature fruit pods is increased. The method has application prospects in the aspects of improving the yield of leguminous crops or cultivating high-quality and high-yield leguminous crop varieties and the like.

The utility model discloses a drip irrigation device for alfalfa cultivation, and relates to the technical field of drip irrigation devices, the drip irrigation device comprises a main pipe, the surface of the main pipe is respectively provided with a drip irrigation assembly and a mounting assembly, the drip irrigation assembly comprises a telescopic hose and a water outlet pipe, one end of the telescopic hose is fixedly connected with the water outlet pipe, and the other end of the telescopic hose is provided with a connector; a plurality of dripping holes are formed in the surface of the water outlet pipe. According to the drip irrigation device for alfalfa cultivation, the drip irrigation assembly is arranged, the water outlet pipe is dragged to the roots of alfalfa through the telescopic hose and buried, the pre-burying work of the drip irrigation device can be completed, during drip irrigation, water is fed into the main pipe, and water flow in the main pipe enters the water outlet pipe through the telescopic hose and flows out through the water dripping holes; during the period, under the action of the filter screen sleeve, broken stones and sand grains under the soil layer can be filtered, blocking of water dripping holes is avoided, the service life of the drip irrigation device is prolonged, and the practicability of the drip irrigation device is improved.

The invention discloses a dry terrace alfalfa double-layer planting method, and relates to the technical field of dry farming planting.The method includes the specific steps that soil preparation and ridging are conducted on a target dry terrace, ridge type upper-layer planting belts and ditch type lower-layer planting belts are formed, and a photo-thermal bearing area and a rainfall runoff collecting area are arranged respectively; layering root limiting arrangement is conducted, and a root system growth area is divided; fully covering a film for water retention laying, and constructing a water collection and supply channel; sowing double-layer alfalfa in the same period; starting a four-dimensional cooperative regulation and control unit, collecting data, and performing unified scheduling on water, fertilizer, gas and heat by using an algorithm; by constructing a furrow double-layer three-dimensional planting structure and matching with full-film-covering water retention, three-dimensional efficient utilization of dry terrace resources is achieved, water and fertilizer competition is avoided, and the water and fertilizer retention capacity is enhanced; through synchronous sowing, four-dimensional cooperative regulation and control, an exclusive water supplementing amount algorithm and synchronous peak shifting harvesting, mechanical harvesting operation requirements are met, damage of mechanical rolling to plants and ridge body structures is avoided, and support is provided for large-scale and mechanical planting of dry terrace alfalfa.

The invention discloses a synthetic flora capable of improving salt tolerance of alfalfa. The synthetic flora consists of a pseudomonas strain EC5, an agrobacterium strain EC17, a streptomyces strain EC15, a paracoccus strain RH39, a pseudomonas strain RH6, a pseudomonas strain RH37, a sporosarcina strain RH34 and a bacillus strain RH32. The synthetic flora provided by the invention has the functions of dissolving organic phosphorus, dissolving inorganic phosphorus, dissolving potassium, producing iron carriers, producing biological membranes, producing IAA and producing ACC deaminase. Seed soaking and germination under the stress of 200 mmol / L and 250 mmol / L NaCl can obviously improve the germination potential, germination rate, germination index, radicle length, germ length and colony seedling embryo fresh weight of alfalfa seeds. Under the stress of 200 mmol / L NaCl, the plant height, stem diameter, leaf area and overground part fresh and dry weight of alfalfa plants can be obviously improved after the seeds are soaked by the synthetic flora.

The present application relates to the prairie repair technical field, especially in kind of degraded prairie repair fungicide, granular repair fungicide and amendment and application thereof.The present application provides the degraded prairie repair fungicide by the preservation number of fluorescent pseudomonas T6 and the preservation number of the alfalfa sinorhizobium NFB5 of CGMCC NO.33618, and the degraded prairie repair fungicide provided by the present application is composed of the preservation number of fluorescent pseudomonas T6 and the preservation number of the alfalfa sinorhizobium NFB5 of CGMCC No.36876.The fluorescent pseudomonas T6 in the degraded prairie repair fungicide provided by the present application and the alfalfa sinorhizobium NFB5 two compound have the synergistic effect, and the repair effect to the degraded prairie is good, and the strain colonization rate is high.The present application relies on the native resources (sheep manure, peat soil, local microorganism and native grass species) of Inner Mongolia grassland, and gives consideration to ecological repair and livestock production, realizes the integration repair of "soil-microorganism-vegetation" three-dimensional synergy, and has important technical innovation value and practical application significance.

The application belongs to the technical field of planting and fertilization, and particularly relates to a diversity planting method for improving phosphorus fertilizer utilization efficiency of sunflower in saline-alkali soil, which solves the technical problem of low phosphorus fertilizer utilization efficiency of the existing sunflower planting method. The method first uses desulfurized gypsum as a soil conditioner, plants sunflower by ridge planting, and plants wild soybean, sueda and alfalfa in diversity, realizes inter-ridge crop coverage to reduce evaporation and suppress salt, cooperates with a composite phosphorus-solubilizing microbial agent containing bacillus subtilis, bacillus megaterium and pseudomonas fluorescens, enhances soil phosphorus-solubilizing microbial activity, and through precise water and fertilizer integrated management during the growth period of the sunflower, according to the needs of different growth periods, urea, potassium sulfate, potassium dihydrogen phosphate and other fertilizers are driped to ensure sufficient and balanced nutrition supply. The diversity planting method significantly improves the phosphorus fertilizer utilization efficiency in saline-alkali soil, is suitable for practical application in saline-alkali soil, and has wide popularization value.

The invention discloses an enterobacter strain and application thereof in improving stress resistance of plants. The invention relates to the field of biological agriculture, and provides bacillus fruticola YJD5-23, and the registration number of the bacillus fruticola YJD5-23 in the General Microbiological Center of the China Committee for Culture Collection of Microorganisms is CGMCC No.36069. The bacillus fruticola YJD5-23 has the advantages that the bacillus fruticola YJD5-23 can be used for preparing the bacillus fruticola YJD5-23; the enterobacter sphaeroides YJD5-23 provided by the invention can improve the resistance of arabidopsis thaliana, alfalfa, corn and soybean to saline-alkali stress, specifically, the dry weight, the fresh weight, the plant length, the leaf area and the chlorophyll content are increased, the oxidation resistance is improved, the wilt is reduced, and the physiological and biochemical indexes are improved. The YJD5-23 microbial inoculum improves the property and activity of saline alkali soil and improves the abundance and composition of microbial communities. Detection shows that the YJD5-23 strain has the capabilities of secreting IAA, ACC deaminase and siderophore, dissolving phosphorus, fixing nitrogen and dissolving potassium. The indexes show that the YJD5-23 microbial inoculum has the potential of growth-promoting bacteria and has important application value in the field of agriculture.

The invention discloses an application of an LAP1 gene or an encoded protein thereof in regulation and control of physical dormancy of plants. It is found that compared with wild medicago truncatula, transgenic medicago truncatula obtained after the LAP1 gene separated from medicago truncatula is subjected to overexpression in medicago truncatula shows phenotypes such as water swelling or / and seed germination rate improvement and the like, and the transgenic medicago truncatula can be applied to the field of medicago truncatula. The physical dormancy of the transgenic medicago truncatula seeds is obviously broken. The method has application prospects in the aspects of promoting plant seed germination, reducing the production cost during seed germination and the like.

The invention relates to the technical field of alfalfa production equipment, and discloses an alfalfa production water and fertilizer integrated irrigation system which comprises a feeding mechanism, one end of the feeding mechanism is communicated with a flow guide mechanism, and the feeding mechanism comprises an outer shell and supporting legs fixedly installed at the lower end of the outer shell; one side of the upper end of the outer shell communicates with a feeding pipe, and one side of the outer shell communicates with a water inlet pipe; the flow guide mechanism comprises a flow guide pipe, and the two ends of the flow guide pipe communicate with the outer shell. Three discharging ports are formed in the side wall of the second circulation pipe, the humidity sensor is inserted into soil, the humidity of the soil is monitored in real time, the humidity sensor is used for monitoring the water content in the soil, fertilizer water enters the flow guide pipe, sequentially flows through the first circulation pipe, the movable barrel, the second circulation pipe and the conical barrel and finally flows into the soil from the three discharging ports and the conical barrel, and the water content in the soil is monitored. Fertilizer and water are irrigated together, integrated system operation is achieved, personnel investment is reduced, and use is convenient.