106 results about "Medicago" patented technology

The invention provides a microbial agent for promoting growth of medicago sativa and application of the microbial agent, and relates to the technical field of agriculture, the microbial agent is prepared from sinorhizobium meliloti MCSWL1, sinorhizobium meliloti MCSD1 and nitrogen-fixing rhizobium sp. MCDC3 according to the mass ratio of 1: 1: 2, and the mass ratio of the sinorhizobium meliloti MCSWL1, the sinorhizobium meliloti MCSD1 to the nitrogen-fixing rhizobium sp. MCDC3 to the nitrogen-fixing rhizobium sp. MCDC3 to the nitrogen-fixing rhizobium sp. MCDC3 is 1: 1: 2. The microbial agent for promoting the growth of the medicago sativa, provided by the invention, can form a close symbiotic relationship with the medicago sativa (particularly grassland No.3), and provides sufficient nitrogen nutrition for the growth of the medicago sativa, so that the soil fertility is effectively improved, and the healthy growth of plants is promoted.

The invention provides bacillus amyloliquefaciens LYZ1125, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum. The preservation number of the bacillus amyloliquefaciens LYZ1125 is CGMCC (China General Microbiological Culture Collection Center) No.31716, and the preservation number of the bacillus amyloliquefaciens LYZ1125 is CGMCC No.31716. The bacillus amyloliquefaciens LYZ1125 disclosed by the invention has relatively good bacteriostatic activity on 11 pathogenic bacteria of six common diseases of alfalfa and verticillium dahliae (separated from oxytropis aureocauda plants) belonging to the same species and different species as the verticillium medicago, and pot experiment shows that the bacillus amyloliquefaciens LYZ1125 can promote the growth of alfalfa root systems and plants; the morbidity of the alfalfa verticillium wilt can be remarkably reduced, the good prevention and treatment effect on the alfalfa verticillium wilt is achieved, and an important theoretical basis is provided for biological prevention and treatment of the alfalfa verticillium wilt (V.alfalfae) LYZ0257).

The invention discloses an accurate water and fertilizer regulation and control technology for an alfalfa seed field, and aims to improve the water and fertilizer utilization efficiency and seed yield of alfalfa by optimizing a water and fertilizer management strategy. The invention provides a precise regulation and control mode based on irrigation amount and fertilization amount coupling, the evaporation amount of a reference crop is calculated by using a Penman-Monteith formula, the optimal water and fertilizer ratio suitable for different alfalfa growth stages is provided based on a pot experiment and a field experiment, a comprehensive evaluation system is constructed, an economic benefit analysis model is combined, and the optimal water and fertilizer ratio is calculated. A scientific basis is provided for popularization and application. According to the technology, the growth indexes (such as plant height and leaf area) of alfalfa are remarkably improved, and the seed quality (such as thousand seed weight and germination rate) is improved. In addition, efficient utilization and sustainable development of resources are achieved, and remarkable economic and ecological benefits are achieved.

The invention relates to an ecological restoration method for a high and cold mine, and belongs to the technical field of ecological restoration. The ecological restoration method for the high-cold mine comprises the step of performing ecological restoration on the high-cold mine by adopting vegetation configuration of shrubs, herbaceous plants A and herbaceous plants B, the herbaceous plants A comprise at least four of elymus dahuricus, siberian wildrye herb, festuca arundinacea, bluegrass, stipa purpurea, ryegrass, frozen artemisia stelleriana and herba artemisiae annuae; the herbaceous plant B comprises at least three of artemisia desertorum, plantain herb, alfalfa, astragalus membranaceus, dandelion and oxytropis philippinarum; the shrub species comprise at least two of sophora moorcroftiana, sabina vulgaris, sea-buckthorn, caragana and buddleia. According to the method, specific vegetation configuration of shrubs, herbaceous plants A and herbaceous plants B is adopted, and soil microorganism resources are recovered quickly; the extreme environment of high and cold mines can be overcome; the ecological system is quickly and efficiently restored, only one-time sowing is needed, and the cost is low; the vegetation and microorganisms interact with each other, so that the survival rate of the vegetation is high.

The invention relates to the technical field of saline-alkali soil treatment, and discloses a light and moderate saline-alkali soil alfalfa seedling protection and strong growth promotion planting method which comprises the following steps: S1, soil pretreatment and root zone microenvironment construction; s2, seed treatment and space-time double-salt-avoiding sowing; s3, microbial inoculum synergistic application; s4, water and fertilizer integration and accurate management; s5, dynamic regulation and control in the growth period, a low-salt root zone microenvironment is constructed through combination of physical obstruction and biological improvement, salt ascending is inhibited by using a slag salt isolation layer, fertility is improved by using an organic layer, and stress resistance is enhanced by adopting a salt-tolerant variety, climate window sowing and aerogel embedding fungicide; precise regulation and control are achieved through water and fertilizer integrated drip irrigation, the stability of a root zone is maintained by combining intertillage and directional topdressing, finally, the seedling emergence uniformity and the seedling stage survival rate are remarkably improved, and robust growth of plants in saline-alkali soil is promoted.

The invention discloses a method for detecting verticillium medicaginis based on an enzyme-mediated double-index amplification technology and a detection kit of the verticillium medicaginis. The invention belongs to the field of biological detection, and particularly relates to a method for detecting verticillium medicaginis based on an enzyme-mediated double-index amplification technology and a detection kit of the verticillium medicaginis. The composition for detecting medicago sativa verticillium comprises a primer F1, a primer R1 and an RNA probe, the nucleotide sequence of the primer F1 is as shown in SEQ ID No: 1, the nucleotide sequence of the primer R1 is as shown in SEQ ID No: 7, and the nucleotide sequence of the RNA probe is as shown in SEQ ID No: 15. The rapid visual detection technology for the verticillium medicaginis EnDEA, which is researched and developed by the invention, is high in specificity, rapid and sensitive, and convenient for detection personnel to carry out rapid detection on the spot, so that the detection time is greatly shortened, the detection cost is reduced, and an important reference is provided for rapid detection of the verticillium medicaginis.

The invention discloses an enterobacter strain and application thereof in improving stress resistance of plants. The invention relates to the field of biological agriculture, and provides Enterobacter sphaeroides YJD-AD2-2, and the enrollment number of the Enterobacter sphaeroides YJD-AD2-2 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms is CGMCC No.29524. The Enterobacter sphaeroides YJD-AD2-2 can be used for preparing the enterobacter sphaeroides YJD-AD2-2. The enterobacter sphaeroides YJD-AD2-2 provided by the invention can improve the resistance of arabidopsis thaliana, alfalfa, corn and rice to salt and alkali stress, specifically, the dry weight, fresh weight, plant length, leaf area and chlorophyll content are increased, the oxidation resistance is improved, wilt is reduced, and physiological and biochemical indexes are improved. The YJD-AD2-2 microbial inoculum improves the property and activity of saline alkali soil and improves the abundance and composition of microbial communities. Detection shows that the YJD-AD2-2 strain has the capabilities of secreting IAA (Indoleacetic Acid), ACC (Acetyl Cellulose) deaminase and siderophore, dissolving phosphorus and fixing nitrogen. The indexes show that the YJD-AD2-2 microbial inoculum has the potential of growth-promoting bacteria and has important application value in the field of agriculture.

The present disclosure relates to Lactiplantibacillus plantarum EL6 for increasing the flavonoids content, and a use thereof, and belongs to the technical field of microbial feeds. The present disclosure is intended to solve the problem that there is no special lactobacillus agent for increasing the flavonoids content during silage fermentation in the prior art. The Lactiplantibacillus plantarum EL6 in the present disclosure is a Gram-positive bacterium, which allows glucose facultative fermentation and has advantages such as acid resistance and high growth rate. In addition, the Lactiplantibacillus plantarum EL6 has an ability to allow the biotransformation of flavonoids, and can significantly increase a content of flavonoids in Medicago silage and promote the release and transformation of bound flavonoids. The Lactiplantibacillus plantarum EL6 can improve a fermentation quality of silage, and has advantages such as low cost, safety, reliability, and easy utilization.

The invention discloses application of a gene MtPrx38 in regulation and control of growth, development and quality of forage grass. The method comprises the following steps: carrying out genome PCR (Polymerase Chain Reaction) identification to obtain homozygotes MtPrx38-1 and MtPrx38-2 of the medicago truncatula MtPrx38 gene mutant; the expression quantity of the MtPrx38 gene detected by RT-PCR (Reverse Transcription-Polymerase Chain Reaction) is obviously reduced, and compared with wild medicago truncatula, the peroxidase activity of the mutant homozygotes MtPrx38-1 and MtPrx38-2 is obviously reduced. Compared with wild medicago truncatula, the MtPrx38 gene mutant has the advantages that the root length, the lateral root number, the plant height, the branch number, the leaf area and the secondary branch number are obviously increased; the flowering time is advanced; the lignin content and the cellulose content are obviously reduced, which indicates that the gene MtPrx38 has the functions of regulating and controlling the growth, development and quality of forage grass. The method has application prospects in the aspects of promoting growth and development of forage grass, improving forage grass quality and the like.

The invention discloses application of alfalfa VIK protein in cultivation of phytophthora root rot resistant plants, and belongs to the technical field of biology. The technical problem to be solved by the invention is how to improve the disease resistance of plants. The VIK protein disclosed by the invention is a protein of which the amino acid sequence is SEQ ID No. 1 or SEQ ID No. 4. Experiments prove that after the phytophthora phytopathogen is inoculated, compared with a wild plant, the mutant obtained by editing the VIK coding gene has the advantages that the disease resistance is enhanced, the survival rate is improved, the fresh weight, the overground part height, the root length and the plant height of the plant are increased, and the relative conductivity of leaves is reduced. Therefore, the resistance of the plant to the phytophthora phytopathogen is improved through the mutation of the VIK protein and the coding gene thereof, the coding gene of the VIK can be targeted through a gene editing technology, a VIK gene knockout plant is obtained, and a plant resisting the phytophthora phytopathogen is cultivated.

The invention belongs to the technical field of soil remediation, and provides a preparation method of a saline-alkali soil remediation agent aiming at the problem of insufficient long-acting stability caused by uncontrollable Ca < 2 + > release of an existing saline-alkali soil remediation agent. The method comprises the following steps: by taking alfalfa residues as raw materials, fermenting to generate acidic fermentation liquor, dissolving soil carbonate, reducing pH, triggering coordinate bond dissociation of chelated calcium gel, realizing pH responsiveness of releasing Ca < 2 + >, and releasing Ca < 2 + > to replace Na < + >; meanwhile, quinonyl on the surface of the modified biochar is used for activating extracellular electron transfer of the Prussella adamsii, and hydroxyl ion secretion is promoted to neutralize salt groups; the alfalfa humification fermentation material and exopolysaccharides secreted by flora are crosslinked through hydrogen bonds to form a cementation layer, soil aggregates are stabilized through a modified charcoal pore framework, calcium slow release balance is regulated and controlled, the problem of local hardening caused by sudden release of Ca < 2 + > is cooperatively solved, and long-acting repair and improvement of the saline-alkali soil are achieved.

The invention discloses a microbial remediation method for improving desert steppe soil quality, which comprises the following steps: sowing grass seeds and applying microbial fertilizer to a pretreated land parcel, sowing 1-5kg of grass seeds per mu, applying 15-45kg of fertilizer per mu, sowing line spacing of 35cm and sowing depth of 2-3cm, and the grass seeds comprise 40-60 parts by weight of Aconitum carmichaeli, 5-15 parts by weight of Astragalus adsurgens and 30-50 parts by weight of Mongolian wheatgrass; the microbial fertilizer comprises nitrogen-fixing bacteria, phosphate solubilizing bacteria and growth-promoting bacteria, the content of organic matters is greater than or equal to 40%, and the pH value is 6.5-7.5; wherein nitrogen-fixing bacteria in the microbial fertilizer are alfalfa rhizobium or soybean rhizobium; the phosphate solubilizing bacteria are pseudomonas fluorescens or pseudomonas putida; the growth promoting bacteria are bacillus subtilis or bacillus licheniformis; the weight ratio of the nitrogen-fixing bacteria to the phosphate solubilizing bacteria to the growth-promoting bacteria is 2: (1-1.5): (1-1.5). By applying the microbial fertilizer and sowing the grass seeds, the physical properties, chemical properties and microbial properties of the soil are effectively improved.

The invention relates to the field of biological agriculture, in particular to application of a gene MtUMAMIT8 to regulation and control of growth, development and quality of forage grass. The biological function of the gene MtUMAMIT8 is identified for the first time, the MtUMAMIT8 gene is screened and locked through multi-omics analysis, the phenotype of a homozygous mutant NF8465 / NF12630 is analyzed, it is proved that the growth of medicago truncatula is hindered but the lignin content is reduced due to down-regulation of the gene MtUMAMIT8, it is clear that the gene MtUMAMIT8 positively regulates the growth and development of medicago truncatula plant height, the branch number, the leaf size and the like and negatively regulates lignin synthesis, and the gene MtUMAMIT8 can be used for identifying the lignin content of the medicago truncatula. And a new gene resource is provided for regulating and controlling the growth, development and quality of forage grass and breeding forage grass varieties with low lignin content.

The application discloses a sequence of Medicago truncatula AP2 / ERF transcription factor ERM1 and application of the sequence in improving mycorrhizal colonization efficiency of leguminous plants and promoting mycorrhizal symbiosis, and relates to the technical field of plant genetic engineering. ERM1 The gene has a significant promoting effect on the symbiosis of alfalfa and arbuscular mycorrhizal fungi, and the application has important significance for clarifying the biological function of ERM1 in the symbiosis of leguminous plants and mycorrhizal fungi, thereby regulating the symbiosis between plants and mycorrhizal fungi, and provides a research foundation and scientific basis for improving the symbiosis efficiency of leguminous crops by using biological strengthening means.

The invention discloses a saline-alkali grassland management method based on ecological rotation and land sharing, which comprises the following steps: equally dividing a target land into a plurality of grid areas, collecting soil samples in different grid areas to measure the pH and total nitrogen content of soil, and dividing the soil into an ecological restoration area, a forage grass production area and an economic plant area according to the measured pH and total nitrogen content of the soil. Suaeda glauca and sesbania are planted in the ecological restoration area, alfalfa and leymus chinensis are planted in the forage grass production area, radix isatidis and purple perilla are planted in the economic plant area, and crop rotation planting is conducted on the three areas with two years as the cycle. According to the design, through complementation of plant communities, the fertilization requirement can be reduced, the soil remediation speed can be increased, grassland rotation management of multi-subject land sharing is achieved through ecological function complementation, the land utilization efficiency can be effectively improved, the grassland production-ecology-life three-generation functions are considered, and sustainable development of the saline-alkali grassland is promoted.

The invention belongs to the technical field of agricultural planting, and provides a method for interplanting triticale and alfalfa in barren land, which comprises the following steps: S1, selecting triticale and alfalfa varieties suitable for local environment; s2, in the period from September to October, soil deep ploughing is conducted on the planting land parcel by 25-30 cm, and triticale seeds and alfalfa seeds are evenly mixed according to the proportion of 7: 1; s3, harvesting triticale in different periods according to utilization purposes, and continuing to reserve alfalfa for growth after harvesting; s4, performing irrigation, disease and pest monitoring, fertilization, weeding and cutting management on the feed triticale and alfalfa; according to the method, crop varieties suitable for the local environment are selected, soil deep ploughing and seed treatment are conducted, reasonable sowing and fertilization are conducted, and fine field management measures are taken, so that efficient utilization of the barren land is achieved; according to the method, the utilization rate of land and the yield of crops are increased, diseases and insect pests are reduced through interplanting and fine management, the soil structure is improved, and the soil fertility is improved.

1. The name of this design product: Label (Yuanshizhiming). 2. Purpose of this design product: used as a label for packaging bottles. 3. The design key point of this appearance design product lies in the combination of shape and pattern. 4. The picture or photo that best illustrates the key points of the design: Main view of Design 1. 5. This design product is a flat product, and the rear, left, right, top and bottom views of Design 1 and Design 2 are omitted. 6. Designate Design 1 as the base design.

The application provides a kind of alfalfa population three-dimensional structure phenotype information extraction method and system. First, according to the ground and wall plane in the original point cloud, the original point cloud is corrected in coordinate;Then according to the reason and characteristics of noise generated by original point cloud, based on statistical filtering removes outlier, based on crown voxel filtering removes redundant point, based on moving least square method removes mixed point;Then according to the characteristics of planting and growth of potted alfalfa population, the alfalfa population is extracted by using straight-through filtering and grid division method, and the single plant is segmented, and then six kinds of structure parameters such as maximum plant height, average plant height, plant height quantile, canopy cover, projected leaf area and three-dimensional volume are extracted.The application realizes the lossless automatic extraction of alfalfa population structure phenotype parameters, and the application is also suitable for three-dimensional structure phenotype information extraction of other population growth plants.

This invention discloses a rapid establishment and high-frequency harvesting method for alfalfa cultivation on desert edges without the need for windbreaks, belonging to the field of sandy land ecological agriculture and forage cultivation technology. This method is suitable for shifting sandy areas on desert edges, eliminating the need for windbreaks. Early-maturing forage oats are sown in single rows in spring. After the oats reach a height of 10-20cm and form a windbreak barrier, drought- and salt-tolerant alfalfa is sown in rows between the rows. Drip or sprinkler irrigation is used, and nitrogen, phosphorus, and potassium fertilizers are reduced by 20%-30% compared to conventional methods. Three to five days after each alfalfa harvest, a nutrient solution containing enzymatically hydrolyzed animal-derived small molecule peptides with a molecular weight <1000Da is applied with irrigation water. The oats are harvested in one go after maturity; the alfalfa is harvested frequently when the plants reach a height of 50-60cm, with 5-6 harvests per year. This invention utilizes oats for temporary wind protection, resulting in a lower than 15% mortality rate from wind and sand during the alfalfa seedling stage, enabling planting in the same year. The hay has a crude protein content of 19%–22%, a yield of over 1200 kg of hay per mu, and can be harvested continuously for 8–10 years. It is suitable for desert edge areas with annual precipitation <100 mm.

The application discloses a method for improving productivity of alfalfa grassland in semi-arid area, which comprises the following steps: sowing in the same period strip sowing mode of planting one row of alfalfa every two rows of feed oat; harvesting mixed forage of alfalfa and feed oat only once in the first year, and not ploughing the land after mowing; in the second year, sowing two rows of feed oat in the alfalfa row without ploughing from late April to early May after the alfalfa turns green; harvesting mixed forage of alfalfa and feed oat in the first harvest in the second year; harvesting alfalfa grass in the second and third harvests; in the third year and the following years, sowing two rows of feed oat in the alfalfa row without ploughing after the alfalfa turns green from late April to early May; in the third year and the following years, harvesting mixed forage of alfalfa and feed oat in the first harvest at the initial flowering period of alfalfa in late June; and harvesting alfalfa grass in the second and third harvests. The application has high utilization rate of natural precipitation, does not need irrigation, has high yield, and can effectively improve the productivity of alfalfa grassland in semi-arid area.

A device for improving the germination rate of alfalfa under continuous cropping, relating to the field of seedling technology; comprising a shell, with seedling trays fixedly installed on the left and right sides of the shell from bottom to top, and a feed cylinder assembly that can move up, down, forward, and backward between the seedling trays inside the shell; the feed cylinder assembly includes a funnel-shaped feed cylinder, with an openable and closable cylinder cover at the top of the feed cylinder, and a rotatable rotating cylinder sleeved on the lower outer side of the feed cylinder, with a horizontal feed guide pipe integrally connected to the bottom of the rotating cylinder, and a feed dispensing through hole at the bottom of the feed guide pipe; a peeling cylinder is rotatably connected between the two ends of the feed guide cylinder, with a gap between the outer side of the peeling cylinder and the inner side of the feed guide cylinder, and a shaft connected between the two ends of the peeling cylinder, with opposing spiral blades connected on both sides of the shaft located at the inner center of the peeling cylinder, an axial discharge port on the outer side of the peeling cylinder, and uniformly distributed protrusions integrally connected to the outer side of the peeling cylinder; and an air conditioner installed on the outer side of the shell.

The invention discloses a cysteine-rich small peptide CYSTM5 gene of medicago truncatula and application of the cysteine-rich small peptide CYSTM5 gene, and provides a method for cultivating a cold-resistant and disease-resistant plant by using the MtCYSTM5 gene, and after the gene is over-expressed in medicago truncatula, the cold resistance and disease resistance of transgenic medicago truncatula are improved; meanwhile, after the gene is inserted into a mutant in medicago truncatula through Tnt1, the gene loses efficacy, and the cold resistance and disease resistance of transgenic medicago truncatula are reduced; and after a mutant plant is infected by a complementary mutant carrier, the cold resistance and disease resistance of the mutant can be recovered. The gene for regulating and controlling cold resistance and disease resistance of medicago truncatula is excavated, and a target gene is provided for cold-resistant and disease-resistant molecular breeding of other pasture or crops.

The application focuses on the field of agricultural informatization and remote sensing application technology, and particularly relates to a kind of alfalfa yield estimation method based on multispectral and physical constraint sample enhancement. The method first acquires unmanned aerial vehicle multispectral image data corresponding to the alfalfa growth period, and lays out field quadrats with equal area in the image coverage area, collects measured data of alfalfa hay yield, leaf area index, chlorophyll content and leaf equivalent water thickness, and constructs an initial sample set. Then, input each parameter data within a reasonable range, generate predicted multispectral samples that meet the physical consistency constraint using the radiation transfer model, to expand the sample space. The measured samples and predicted samples are fused to construct an alfalfa hay yield estimation training data set. Based on the training data set, a mapping model between unmanned aerial vehicle multispectral features and alfalfa hay yield is established, and the alfalfa hay yield is estimated through machine learning.

The invention discloses a pasture weed identification method based on a convolutional neural network and application thereof. The method comprises the following steps: respectively acquiring image data of an alfalfa field before sowing and when seedlings turn green; on the basis of alfalfa field image data before sowing, weeds are judged through color features; on the basis of alfalfa field image data when seedlings turn green, dividing weeds into monocotyledonous weeds and dicotyledonous weeds according to the types of the weeds, and performing key feature acquisition on alfalfa and dicotyledonous weeds; category labels are attached to the initial alfalfa and weeds based on the key features, and a training set and a test set are divided; and constructing a pasture weed identification model based on the convolutional neural network model, training the convolutional neural network through the training set, and selecting the convolutional neural network model of the corresponding parameter with the highest identification accuracy. The method has the advantages that the weeds are classified, key features are extracted for alfalfa and dicotyledonous weeds, and efficient and accurate recognition of the weeds in the alfalfa field is achieved through CNN model training.

The invention discloses an application of a CKX gene in regulating alfalfa root systems, comprising the following steps: step 1, preparing a transgenic wild-type alfalfa, overexpressing the MtCKX1 gene in the alfalfa, and obtaining a transgenic plant; the cDNA sequence and amino acid sequence of the MtCKX1 gene are sequence 1 and sequence 2 in the sequence table; step 2, comparing the root systems of the transgenic alfalfa plant with those of the wild-type plant, and the root systems of the transgenic alfalfa plant are larger than those of the wild-type plant; the cytokinin content of the transgenic plant is suppressed, and the root systems of the transgenic plant are strengthened and enlarged; cytokinin has a negative regulatory effect on the root growth of the plant, and after the MtCKX1 gene is overexpressed, the cytokinin is suppressed, and the root system of the plant is strengthened and enlarged, thereby effectively fixing the soil and preventing soil loss.

The invention discloses alfalfa seed propagation equipment and method, and belongs to the field of leguminous plant planting, the alfalfa seed propagation equipment comprises a base and further comprises a diffusion assembly, the diffusion assembly comprises a fixing plate fixedly installed on the upper portion of the base, and a discharging frame is fixedly installed on the lower portion of the base; a discharging opening communicated with the discharging frame is formed in the fixing plate, a movable cavity is formed in the inner cavity wall of the discharging opening, a gear ring is rotationally installed in the movable cavity, a partition plate is fixedly installed on the inner wall of the gear ring, and a connecting rod is fixedly connected to the bottom of the partition plate; according to the alfalfa seeding device, alfalfa seeds can be uniformly arranged in seeding pits, and the situation that the alfalfa seeds are seeded too densely to cause intense competition and influence the growth and development of alfalfa can be avoided, so that the consistency of the spacing between planted plants can be effectively improved, and the growth of the alfalfa and the balance of light, nutrients and moisture can be effectively improved.

The invention relates to the technical field of alfalfa cultivation, and discloses a device for cultivating drought-resistant and salt-alkali-tolerant alfalfa varieties, comprising a concrete base and a concrete ring platform, wherein an annular cultivation area is formed between the concrete base and the concrete ring platform, the annular cultivation area is used for cultivating annularly distributed alfalfa, and a fertilization area is formed between adjacent annular alfalfa; a vertical tower is arranged on the concrete base; the vertical tower is connected with a cross rod through a ball bearing; a driving trolley is arranged at the free end of the cross rod, the driving trolley drives the cross rod to generate a circular rotation, and the driving trolley rolls along the concrete ring platform; there is also a silo for storing alfalfa seeds; there is also a movable and rotatable shovel head, the shovel head is used for grooving the land, so that the alfalfa seeds fall under the soil layer turned up by the shovel head, and a feeding pipe is used for sucking the alfalfa seeds in the silo; by arranging the shovel head, the present application can regularly loosen the soil in the fertilization area in the later stage, improve the soil quality, make the alfalfa grow in multiple crops, and increase the yield of the alfalfa.

The present invention discloses a saline-alkali grassland management method based on ecological rotation and land sharing. The target plot is first divided into multiple grid areas. Soil samples are collected from different grid areas to determine the soil pH and total nitrogen content. Then, based on the measured soil pH and total nitrogen content, the area is divided into an ecological restoration area, a forage production area, and an economic plant area. Salicornia and Sesbania are planted in the ecological restoration area, alfalfa and Leymus chinensis are planted in the forage production area, and Isatis indigotica and Perilla are planted in the economic plant area. Crops are rotated in the three areas on a two-year cycle. The above design can reduce fertilization requirements and increase soil remediation speed through the complementarity of plant communities. It can achieve multi-subject land sharing grassland rotation management through the complementarity of ecological functions, effectively improve land use efficiency, take into account the "three lives" of grassland production, ecology, and life, and promote the sustainable development of saline-alkali grassland.

This invention relates to a method for creating late-flowering, high-yielding alfalfa, characterized by the following steps: S1: designing and synthesizing targeted alfalfa MvFT S1: Construct the gRNA of the gene; S2: Construct a CRISPR / Cas9 gene knockout vector containing the gRNA expression cassette described in S1; S3: Transform the knockout vector described in S2 into Agrobacterium tumefaciens; S4: Transform explants of alfalfa cultivars into Agrobacterium tumefaciens as described in S3; S5: Screen, differentiate, and regenerate the explants transformed in S4 to obtain transgenic plants; S6: Perform molecular and phenotypic identification on the transgenic plants to screen for... MvFT A late-flowering, high-yielding germplasm with homozygous gene knockout and significantly delayed flowering time. This invention reveals for the first time... MvFT The key functions of genes in the regulation of flowering in alfalfa were investigated, and new late-flowering, high-yielding alfalfa germplasm was successfully created, providing valuable germplasm resources for breeding new high-yielding and high-quality alfalfa varieties.

The invention is applicable to the technical field of sowing, and provides an alfalfa seed planting device, comprising a planting frame; a lifting part is symmetrically screwed on a bidirectional screw rod; a plurality of seed taking parts that are slidably matched with corresponding seed storage tanks are fixed on one lifting part; a plurality of seed delivery parts that are slidably matched with corresponding insertion tubes are fixed on the other lifting part; a sliding part that is slidably provided at the bottom of the insertion tube and is adapted to the seed delivery part; an exhaust component is rotatably provided inside a fixed box; the device extracts the air inside the seed taking tube through an exhaust fan to form a negative pressure environment inside the tube, thereby adsorbing the alfalfa seeds in the seed storage tank into an adsorption groove, controlling the seed taking tube to rise and separate from the discharge port, and keeping the number of alfalfa seeds adsorbed in each group of adsorption grooves on the outer peripheral side of the seed taking tube basically consistent, thereby releasing the negative pressure inside the seed taking tube, and the alfalfa seeds in the adsorption groove are dropped into the insertion tube through the seed delivery part under the action of gravity, thereby realizing quantitative sowing and improving the emergence rate and survival rate of sowing.