The invention discloses a treatment method for increasing the
germination rate of
medicago truncatula seeds. The treatment method comprises the steps of after disinfecting the
medicago truncatula seeds, soaking the
medicago truncatula seeds by using a 0.1 mmol / L
sodium nitroprusside solution for 48h, and culturing the
medicago truncatula seeds in a dark place for 7 days at the temperature of 25+ / -1 DEG C and 12h illumination (3000LX) / 12h to promote seed
germination. The treatment method for increasing the
germination rate of the
medicago truncatula seeds has the beneficial effects that the 0.1mmol / L
sodium nitroprusside (SNP) solution is used for treating the
medicago truncatula seeds so that the germination is remarkably promoted, and the germination rate reaches 62.14 percent, and is increased by 54.47 percent in comparison with those of a contrast group.
Sodium nitroprusside is nontoxic at a low concentration, few in consumption amount when being used for treating the seeds, economical, short in time and high in effect taking speed; the seeds are not damaged, and the method is simple and is not influenced by factors such as season, weather, and
natural hazard. By using the method, the problem of low germination rate of the medicago truncatula seeds can be solved, and
germplasm resources with good
germplasm are provided for biological study of legumes and production of medicago truncatula.