56 results about "Medicago truncatula" patented technology

The invention relates to isolated DMI11 genes from Medicago truncatula which play a major role both in the early steps of Nod factor signaling that trigger several key developmental responses in the host plant and in the establishment of mycorrhizal symbiosis. The invention also relates to transgenic plants and plant cells expressing the DMI1 protein for increased root nodulation, and methods for transforming plants with a M. truncatula DMI1 gene.

The invention relates to a method for improving salt stress tolerance of medicago truncatula. The method comprises the steps: (1) preparing a solid CM complete medium for piriformospora indica; (2) culturing the piriformospora indica and collecting a spore suspension; (3) inoculating the piriformospora indica to the medicago truncatula; and (4) transplanting medicago truncatula seedlings and carrying out later-stage management. According to the method, the piriformospora indica spore suspension is adopted to soak seeds in advance, so that the piriformospora indica can be parasitized to surfaces of the medicago truncatula seeds, the seeds are promoted to germinate ahead of time, and the germination percentage of the seeds is remarkably increased (increased to 95.0% from the original 80.0%); the biological yield of the medicago truncatula can be remarkably increased; and through parasitizing the piriformospora indica to roots of the medicago truncatula, the accumulation of sodium ions in the roots of the medicago truncatula can be lowered, and thus the salt stress tolerance of the medicago truncatula is improved.

The invention provides nematode-resistant transgenic plants and seed comprising polynucleotides encoding Medicago truncatula cysteine cluster proteins which comprise no more than four cysteine residues in the respective mature peptides. The invention also provides methods of producing transgenic plants with increased resistance to soybean cyst nematode and expression vectors for use in such methods.

The invention discloses application of an MtUNUSUAL FLORAL ORGANS gene to the regulation and the control on the leaflet quantity and the leaf-stem ratio of a leguminous plant. According to the application, a knockout mutant strain of the MtUNUSUAL FLORAL ORGANS gene in medicago truncatula is separated and obtained for the first time; proven through an experiment, the gene participates in the regulation and the control on the leaflet number and the leaf-stem ratio of the leguminous plant; a transgenic leguminous plant whose leaflet number and leaf-stem ratio are higher than those of a wild type plant is obtained through the way of over-expressing the gene; the MtUNUSUAL FLORAL ORGANS gene is over-expressed in the medicago truncatula of a compound-leaf species for the first time; the quantity of leaflets of 95 percent of compound leaves can be increased; moreover, the leaf-stem ratio can be increased by approximately 30 percent. The gene is proven to participate in the regulation and the control on the leaflet quantity and the leaf-stem ratio of the leguminous plant; the transgenic leguminous plant whose leaflet number and leaf-stem ratio are higher than those of the wild type plant can be obtained through over-expressing the gene; indicatively, after being implemented, the application is about to be used for creating a novel leguminous forage plant, can be used for subsequent forage quality, and has important significance to Chinese prataculture production.

The invention provides a pair of primers, a kit containing the same, a use and a method for detecting ecotypes A17 and R108 of Medicago truncatula, and relates to the field of biotechnology. The primer pair comprises primer NST480‑F and primer NST480‑R, which have the sequences shown in SEQ ID NO.1 and SEQ ID NO.2 respectively, and can simultaneously amplify DNA fragments of different fragment lengths of Medicago truncatula ecotypes A17 and R108 , so one amplification can distinguish whether the test sample contains Medicago truncatula ecotypes A17 and R108. Using the above primers to detect Medicago truncatula ecotypes A17 and R108 has the characteristics of strong sensitivity, high accuracy, strong repeatability, fast and convenient, and alleviates the lack of a fast and accurate method for detection and identification in the prior art. Problems with Medicago truncatula ecotypes A17 and R108.

The invention discloses a stigma exposed gene of the flower organ of the plant Medicago truncatula and its coded protein and application. The gene is a DNA molecule as described in any of (a1)-(a4): (a1) the DNA molecule whose coding region is shown in SEQ ID NO.1; (a2) the DNA molecule shown in SEQ ID NO.3; (a3) a DNA molecule that hybridizes to the DNA sequence defined by (a1) or (a2) and encodes the protein of claim 1 under stringent conditions; (a4) is derived from Medicago truncatula and is combined with (a1) or (a2) or ( a3) A DNA molecule with more than 90% homology to the defined DNA sequence. The gene of the invention can control the exposure of plant stigma and the fusion of petals; it lays a theoretical basis for the study of CABL2 gene regulating the exposure of stigma in plants.

The invention discloses a transcription factor gene that regulates the synthesis of plant flavonoids. The transcription factor gene is the MtbZIP10 gene identified from the leguminous plant Medicago truncatula, and its gene sequence is shown in SEQ ID NO.1. Moreover, the present invention discloses the function of the gene and its application. The advantage of the present invention is that the transcription factor gene that can positively regulate the synthesis of flavonoid compounds is cloned from the leguminous plant Medicago truncatula, and its function is systematically identified, and it is found that the gene can activate the biosynthesis pathway of flavonoids Multiple genes that increase the content of flavonoid compounds.