13 results about "Medicago truncatula" patented technology

The invention relates to the field of biological agriculture, in particular to application of a gene MtUMAMIT8 to regulation and control of growth, development and quality of forage grass. The biological function of the gene MtUMAMIT8 is identified for the first time, the MtUMAMIT8 gene is screened and locked through multi-omics analysis, the phenotype of a homozygous mutant NF8465 / NF12630 is analyzed, it is proved that the growth of medicago truncatula is hindered but the lignin content is reduced due to down-regulation of the gene MtUMAMIT8, it is clear that the gene MtUMAMIT8 positively regulates the growth and development of medicago truncatula plant height, the branch number, the leaf size and the like and negatively regulates lignin synthesis, and the gene MtUMAMIT8 can be used for identifying the lignin content of the medicago truncatula. And a new gene resource is provided for regulating and controlling the growth, development and quality of forage grass and breeding forage grass varieties with low lignin content.

The application discloses a sequence of Medicago truncatula AP2 / ERF transcription factor ERM1 and application of the sequence in improving mycorrhizal colonization efficiency of leguminous plants and promoting mycorrhizal symbiosis, and relates to the technical field of plant genetic engineering. ERM1 The gene has a significant promoting effect on the symbiosis of alfalfa and arbuscular mycorrhizal fungi, and the application has important significance for clarifying the biological function of ERM1 in the symbiosis of leguminous plants and mycorrhizal fungi, thereby regulating the symbiosis between plants and mycorrhizal fungi, and provides a research foundation and scientific basis for improving the symbiosis efficiency of leguminous crops by using biological strengthening means.

The invention discloses a cysteine-rich small peptide CYSTM5 gene of medicago truncatula and application of the cysteine-rich small peptide CYSTM5 gene, and provides a method for cultivating a cold-resistant and disease-resistant plant by using the MtCYSTM5 gene, and after the gene is over-expressed in medicago truncatula, the cold resistance and disease resistance of transgenic medicago truncatula are improved; meanwhile, after the gene is inserted into a mutant in medicago truncatula through Tnt1, the gene loses efficacy, and the cold resistance and disease resistance of transgenic medicago truncatula are reduced; and after a mutant plant is infected by a complementary mutant carrier, the cold resistance and disease resistance of the mutant can be recovered. The gene for regulating and controlling cold resistance and disease resistance of medicago truncatula is excavated, and a target gene is provided for cold-resistant and disease-resistant molecular breeding of other pasture or crops.

The invention discloses application of a gene MtPrx38 in regulation and control of growth, development and quality of forage grass. The method comprises the following steps: carrying out genome PCR (Polymerase Chain Reaction) identification to obtain homozygotes Prx38-1 and Prx38-2 of the medicago truncatula MtPrx38 gene mutant; rT-PCR (reverse transcription-polymerase chain reaction) detection shows that the expression quantity of the MtPrx38 gene is obviously reduced, and compared with wild medicago truncatula, the peroxidase activity of the mutant homozygotes Prx38-1 and Prx38-2 is obviously reduced. Compared with wild medicago truncatula, the MtPrx38 gene mutant has the advantages that the root length, the lateral root number, the plant height, the branch number, the leaf area and the secondary branch number are obviously increased; the flowering time is advanced; the lignin content and the cellulose content are obviously reduced, which indicates that the gene MtPrx38 has the functions of regulating and controlling the growth, development and quality of forage grass. The method has application prospects in the aspects of promoting growth and development of forage grass, improving forage grass quality and the like.

The present application relates to the field of biological agriculture, and in particular to the application of the gene MtUMAMIT8 in regulating the growth and development and quality of forage grass. The biological function of the gene MtUMAMIT8 is identified for the first time, the MtUMAMIT8 gene is screened and locked through multi-omics analysis, and the phenotype of the homozygous mutant NF8465 / NF12630 is analyzed, confirming that the down-regulation of the gene MtUMAMIT8 leads to the growth of Medicago truncatula being hindered but the lignin content being reduced, and that the gene MtUMAMIT8 positively regulates the growth and development of the plant height, branch number and leaf size of Medicago truncatula and negatively regulates the synthesis of lignin, thereby providing a new gene resource for regulating the growth and development and quality of forage grass and breeding forage grass varieties with low lignin content.

The invention discloses a medicago truncatula MtSPP1 gene and application thereof. The nucleotide sequence of the medicago truncatula MtSPP1 gene is as shown in SEQ ID NO.1, and the amino acid sequence of protein expressed by the gene is as shown in SEQ ID NO.2. The medicago truncatula MtSPP1 gene has a nucleotide sequence as shown in SEQ ID NO.1. The medicago truncatula MtSPP1 gene is applied to increase of the soybean yield and the nodulation number. The MtSPP1 transgene provided by the invention can obviously improve the yield of stems and leaves of the overground part of the soybean, and has important application value in molecular breeding of feed type soybean. The MtSPP1 transgene can obviously improve the grain yield of soybeans, and has important application value in molecular breeding of oil and edible soybeans. The MtSPP1 transgene can significantly increase the number of root nodules of soybeans, has important application value in molecular breeding of soybeans for green manure, and is suitable for popularization and application.

Recombinant DNA molecules and constructs useful for modulating gene expression in plants, including molecules derived from Medicago truncatula sequences, are provided. Plants, plant cells, plant parts, and seeds comprising recombinant DNA molecules operably linked to heterologous transcribable DNA molecules are further provided, as are methods of their use.

The application relates to the field of biological technology, in particular to application of cyclic adenosine monophosphate in promoting biomass increase of pasture and root nodule, wherein the cyclic adenosine monophosphate significantly improves the fresh weight biomass of the above-ground part, the root part and the single-plant root system root nodule of Medicago truncatula inoculated with rhizobium, and the difference is significant.

This invention relates to the field of microbial technology and provides a highly efficient symbiotic nodulation strain of *Rhizobium sinense* 98002 and its applications. *Rhizobium sinense* 98002 was deposited on January 9, 2026, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 37337. The strain 98002 provided by this invention can not only form efficient symbiotic nodules with *Alfalfa* (from which it was isolated) but also achieve good symbiosis with the model plant *Alfalfa truncatum*, broadening its application scenarios. It can be used as a core strain to prepare highly efficient leguminous inoculants for agricultural production, reducing the use of chemical nitrogen fertilizers and improving the soil ecological environment.

The utility model discloses a simple threshing device for medicago truncatula so as to solve the technical problems that an existing threshing device is inconvenient to operate, low in efficiency and insufficient in safety. The inner bottom surface of the tray is provided with a lower rubbing surface, the bottom of the rubbing plate is provided with an upper rubbing surface, two sides of the top of the tray are provided with slideways, the upper part of the rubbing plate is connected with a cross beam, the rubbing plate is connected with the cross beam through a connecting rod, the cross beam is connected with the slideways in a sliding manner, and the cross beam is provided with a threaded hole. Threads corresponding to the threaded holes are arranged on the connecting rod, and the washboard is rotatably connected with the bottom of the connecting rod. The device has the advantages of being convenient to operate, high in working efficiency and good in safety.

The invention discloses an application of an LAP1 gene or an encoded protein thereof in regulation and control of physical dormancy of plants. It is found that compared with wild medicago truncatula, transgenic medicago truncatula obtained after the LAP1 gene separated from medicago truncatula is subjected to overexpression in medicago truncatula shows phenotypes such as water swelling or / and seed germination rate improvement and the like, and the transgenic medicago truncatula can be applied to the field of medicago truncatula. The physical dormancy of the transgenic medicago truncatula seeds is obviously broken. The method has application prospects in the aspects of promoting plant seed germination, reducing the production cost during seed germination and the like.

The invention relates to application of knockout of an MtGH9-24 gene in seed dormancy control, seed dormancy control refers to breaking of seed dormancy, seed epidermis shows that seed coats are incomplete in development and fine cracks appear on the surfaces of the seed coats, and seeds are medicago truncatula seeds. After the MtGH9-24 gene is knocked out, the germination rate of mutant seeds reaches 100% within five days under proper conditions, and wild seeds cannot be imbibed and germinated completely within the same period. The technology provides an efficient and reliable genetic improvement means for solving the problems of low germination rate, delayed germination and the like of medicago truncatula and other hard-seed leguminous seeds. The key function of the MtGH9-24 gene in regulating physical dormancy of medicago truncatula seeds is disclosed for the first time, important candidate genes and molecular tools are provided for cultivating new medicago truncatula varieties with low dormancy and high germination rate in the future, and important theoretical value and application potential are achieved.

The invention discloses an ABA (abscisic acid) related protein CAR4 gene of a C2 structural domain of alfalfa and application of the ABA related protein CAR4 gene. The cDNA sequence MsCAR4 of the alfalfa C2 structural domain ABA related protein CAR4 gene is cloned from alfalfa, and research finds that after the gene is over-expressed in alfalfa, branches are reduced, flowering is advanced, and the yield is reduced; meanwhile, after the gene is interfered in medicago sativa, branches are increased, flowering is delayed, and the yield is improved. According to the invention, genes for regulating late flowering of medicago sativa and promoting branch growth are excavated and related functional studies are carried out, so that a theoretical foundation can be laid for variety improvement of medicago sativa and breeding of germplasm resources.