23 results about "Medicago truncatula" patented technology

The invention relates to the field of biological agriculture, in particular to application of a gene MtUMAMIT8 to regulation and control of growth, development and quality of forage grass. The biological function of the gene MtUMAMIT8 is identified for the first time, the MtUMAMIT8 gene is screened and locked through multi-omics analysis, the phenotype of a homozygous mutant NF8465 / NF12630 is analyzed, it is proved that the growth of medicago truncatula is hindered but the lignin content is reduced due to down-regulation of the gene MtUMAMIT8, it is clear that the gene MtUMAMIT8 positively regulates the growth and development of medicago truncatula plant height, the branch number, the leaf size and the like and negatively regulates lignin synthesis, and the gene MtUMAMIT8 can be used for identifying the lignin content of the medicago truncatula. And a new gene resource is provided for regulating and controlling the growth, development and quality of forage grass and breeding forage grass varieties with low lignin content.

The application discloses a sequence of Medicago truncatula AP2 / ERF transcription factor ERM1 and application of the sequence in improving mycorrhizal colonization efficiency of leguminous plants and promoting mycorrhizal symbiosis, and relates to the technical field of plant genetic engineering. ERM1 The gene has a significant promoting effect on the symbiosis of alfalfa and arbuscular mycorrhizal fungi, and the application has important significance for clarifying the biological function of ERM1 in the symbiosis of leguminous plants and mycorrhizal fungi, thereby regulating the symbiosis between plants and mycorrhizal fungi, and provides a research foundation and scientific basis for improving the symbiosis efficiency of leguminous crops by using biological strengthening means.

The invention discloses a cysteine-rich small peptide CYSTM5 gene of medicago truncatula and application of the cysteine-rich small peptide CYSTM5 gene, and provides a method for cultivating a cold-resistant and disease-resistant plant by using the MtCYSTM5 gene, and after the gene is over-expressed in medicago truncatula, the cold resistance and disease resistance of transgenic medicago truncatula are improved; meanwhile, after the gene is inserted into a mutant in medicago truncatula through Tnt1, the gene loses efficacy, and the cold resistance and disease resistance of transgenic medicago truncatula are reduced; and after a mutant plant is infected by a complementary mutant carrier, the cold resistance and disease resistance of the mutant can be recovered. The gene for regulating and controlling cold resistance and disease resistance of medicago truncatula is excavated, and a target gene is provided for cold-resistant and disease-resistant molecular breeding of other pasture or crops.

The invention discloses application of a gene MtPrx38 in regulation and control of growth, development and quality of forage grass. The method comprises the following steps: carrying out genome PCR (Polymerase Chain Reaction) identification to obtain homozygotes Prx38-1 and Prx38-2 of the medicago truncatula MtPrx38 gene mutant; rT-PCR (reverse transcription-polymerase chain reaction) detection shows that the expression quantity of the MtPrx38 gene is obviously reduced, and compared with wild medicago truncatula, the peroxidase activity of the mutant homozygotes Prx38-1 and Prx38-2 is obviously reduced. Compared with wild medicago truncatula, the MtPrx38 gene mutant has the advantages that the root length, the lateral root number, the plant height, the branch number, the leaf area and the secondary branch number are obviously increased; the flowering time is advanced; the lignin content and the cellulose content are obviously reduced, which indicates that the gene MtPrx38 has the functions of regulating and controlling the growth, development and quality of forage grass. The method has application prospects in the aspects of promoting growth and development of forage grass, improving forage grass quality and the like.

The present application relates to the field of biological agriculture, and in particular to the application of the gene MtUMAMIT8 in regulating the growth and development and quality of forage grass. The biological function of the gene MtUMAMIT8 is identified for the first time, the MtUMAMIT8 gene is screened and locked through multi-omics analysis, and the phenotype of the homozygous mutant NF8465 / NF12630 is analyzed, confirming that the down-regulation of the gene MtUMAMIT8 leads to the growth of Medicago truncatula being hindered but the lignin content being reduced, and that the gene MtUMAMIT8 positively regulates the growth and development of the plant height, branch number and leaf size of Medicago truncatula and negatively regulates the synthesis of lignin, thereby providing a new gene resource for regulating the growth and development and quality of forage grass and breeding forage grass varieties with low lignin content.

The invention discloses a medicago truncatula MtSPP1 gene and application thereof. The nucleotide sequence of the medicago truncatula MtSPP1 gene is as shown in SEQ ID NO.1, and the amino acid sequence of protein expressed by the gene is as shown in SEQ ID NO.2. The medicago truncatula MtSPP1 gene has a nucleotide sequence as shown in SEQ ID NO.1. The medicago truncatula MtSPP1 gene is applied to increase of the soybean yield and the nodulation number. The MtSPP1 transgene provided by the invention can obviously improve the yield of stems and leaves of the overground part of the soybean, and has important application value in molecular breeding of feed type soybean. The MtSPP1 transgene can obviously improve the grain yield of soybeans, and has important application value in molecular breeding of oil and edible soybeans. The MtSPP1 transgene can significantly increase the number of root nodules of soybeans, has important application value in molecular breeding of soybeans for green manure, and is suitable for popularization and application.

Recombinant DNA molecules and constructs useful for modulating gene expression in plants, including molecules derived from Medicago truncatula sequences, are provided. Plants, plant cells, plant parts, and seeds comprising recombinant DNA molecules operably linked to heterologous transcribable DNA molecules are further provided, as are methods of their use.

The present invention discloses a Medicago truncatula leaf development protein, its coding gene and applications. First, through sequence analysis, the present invention clones the gene related to plant leaf development from wild-type Medicago truncatula R108, MtPIF4 then overexpresses the gene in Medicago truncatula through transgenic technology, and MtPIF4 identifies and screens out the stably inherited transgenic MtPIF4 Medicago truncatula lines. Finally, through phenotypic analysis of the transgenic MtPIF4 Medicago truncatula lines, it is found that compared with the wild-type R108, the transgenic MtPIF4 Medicago truncatula lines show obvious phenotypes of larger leaves, thicker main stems, increased number of branches and increased plant height. The present invention lays a theoretical foundation for studying the MtPIF4 gene regulating leaf development in plants.

The present invention provides an MtPRP4 gene isolated from Medicago truncatula, a protein encoded therein, and an application thereof, and belongs to the field of genetic engineering. The MtPRP4 gene is isolated from Medicago truncatula, and the nucleotide sequence of the MtPRP4 gene is shown in SEQ ID NO: 1, and the MtPRP4 gene belongs to a PRP gene; the CDS sequence of the MtPRP4 gene is shown in SEQ ID NO: 2; and the amino acid sequence of the protein encoded by the MtPRP4 gene is shown in SEQ ID NO: 3. The present invention isolates the MtPRP4 gene from Medicago truncatula for the first time, and verifies through experiments the function of the MtPRP4 gene in regulating the growth and development of Medicago truncatula and enhancing abiotic stress resistance, thus filling a gap in the research on PRP genes in Medicago truncatula and providing a new perspective for understanding the function of PRP genes in legumes.

The invention discloses application of an LNC1 gene in regulating and controlling plant types of medicago truncatula or soybean plants, and belongs to the technical field of plant type gene regulation and control. According to related tests, an LNC1-alfalfa gene in medicago truncatula and an LNC1a gene and an LNC1b gene in soybean are homologous LNC1 genes, and the petiole included angle of the medicago truncatula plant and the petiole included angle of the soybean plant can be remarkably increased through function deletion of the LNC1-alfalfa gene and simultaneous function deletion of the LNC1a gene and the LNC1b gene respectively; namely, the change of the petiole included angle of the leguminous plant medicago truncatula and the soybean plant can be realized through the function deletion of the homologous LNC1 gene, so that the plant type regulation and control are realized, and the gene has a wide application prospect in the aspects of leguminous plant type regulation and control and the like.

The application relates to the field of biological technology, in particular to application of cyclic adenosine monophosphate in promoting biomass increase of pasture and root nodule, wherein the cyclic adenosine monophosphate significantly improves the fresh weight biomass of the above-ground part, the root part and the single-plant root system root nodule of Medicago truncatula inoculated with rhizobium, and the difference is significant.

This invention discloses the application of the MrDNAJB13 gene in regulating the drought resistance of hairy roots in alfalfa beans, belonging to the field of biotechnology. This invention discovers that overexpression of the MrDNAJB13 gene yields hairy roots with enhanced drought resistance. Furthermore, this invention constructs a MrDNAJB13 gene overexpression vector and a hairy root transformation method for alfalfa bean hairy root systems. The transformation method of this invention for alfalfa bean hairy root transformation can serve as a biotechnological means for studying the function of drought-resistant genes in alfalfa beans, and may be extended to other fields, including the study of stress resistance mechanisms, laying the foundation for the verification of alfalfa bean gene function and genetic selection.

This invention relates to the field of microbial technology and provides a highly efficient symbiotic nodulation strain of *Rhizobium sinense* 98002 and its applications. *Rhizobium sinense* 98002 was deposited on January 9, 2026, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 37337. The strain 98002 provided by this invention can not only form efficient symbiotic nodules with *Alfalfa* (from which it was isolated) but also achieve good symbiosis with the model plant *Alfalfa truncatum*, broadening its application scenarios. It can be used as a core strain to prepare highly efficient leguminous inoculants for agricultural production, reducing the use of chemical nitrogen fertilizers and improving the soil ecological environment.

The utility model discloses a simple threshing device for medicago truncatula so as to solve the technical problems that an existing threshing device is inconvenient to operate, low in efficiency and insufficient in safety. The inner bottom surface of the tray is provided with a lower rubbing surface, the bottom of the rubbing plate is provided with an upper rubbing surface, two sides of the top of the tray are provided with slideways, the upper part of the rubbing plate is connected with a cross beam, the rubbing plate is connected with the cross beam through a connecting rod, the cross beam is connected with the slideways in a sliding manner, and the cross beam is provided with a threaded hole. Threads corresponding to the threaded holes are arranged on the connecting rod, and the washboard is rotatably connected with the bottom of the connecting rod. The device has the advantages of being convenient to operate, high in working efficiency and good in safety.

The invention discloses an application of an LAP1 gene or an encoded protein thereof in regulation and control of physical dormancy of plants. It is found that compared with wild medicago truncatula, transgenic medicago truncatula obtained after the LAP1 gene separated from medicago truncatula is subjected to overexpression in medicago truncatula shows phenotypes such as water swelling or / and seed germination rate improvement and the like, and the transgenic medicago truncatula can be applied to the field of medicago truncatula. The physical dormancy of the transgenic medicago truncatula seeds is obviously broken. The method has application prospects in the aspects of promoting plant seed germination, reducing the production cost during seed germination and the like.

The invention discloses a plant lateral branch development related protein as well as a coding gene and application thereof. The invention provides a DSI1 protein derived from medicago truncatula and related to plant lateral branch development, the amino acid sequence of the DSI1 protein is as shown in SEQ ID NO.1, the nucleotide sequence of CDS of the coding gene of the DSI1 protein is as shown in SEQ ID NO.2, and the nucleotide sequence of the genome sequence of the coding gene of the DSI1 protein is as shown in SEQ ID NO.3. The invention further provides application of the DSI1 protein related to plant lateral branch development and the coding gene or the genome sequence of the DSI1 protein in regulation and control of plant lateral branch development, which comprises the following steps: mutating the coding gene of the DSI1 protein in a plant to cause defects in normal functions of the DSI1 protein, or interfering normal expression of the coding gene of the DSI1 protein, so that the DSI1 protein related to plant lateral branch development is obtained. The number of lateral branches of plants is reduced, and the number of inflorescences is increased.

The present invention belongs to the technical fields of genetic engineering and salt-tolerant plant breeding, and specifically relates to an alfalfa gene MtZW1 and its application in plant salt tolerance. Specifically, the present invention discovered for the first time a gene MtZW1 that negatively regulates the salt tolerance of Medicago truncatula. Studies have shown that the loss of function of the gene MtZW1 can significantly affect the salt tolerance phenotype of Medicago truncatula. Compared with wild-type plants, the survival rate of the gene mutant plants after treatment with 100mM NaCl was significantly improved. The present invention not only clarifies the mechanism of action of the gene MtZW1 in plant salt tolerance, but also provides new tools and methods for improving crop salt tolerance using genetic engineering methods, which has important scientific significance and practical application prospects.

The invention relates to application of knockout of an MtGH9-24 gene in seed dormancy control, seed dormancy control refers to breaking of seed dormancy, seed epidermis shows that seed coats are incomplete in development and fine cracks appear on the surfaces of the seed coats, and seeds are medicago truncatula seeds. After the MtGH9-24 gene is knocked out, the germination rate of mutant seeds reaches 100% within five days under proper conditions, and wild seeds cannot be imbibed and germinated completely within the same period. The technology provides an efficient and reliable genetic improvement means for solving the problems of low germination rate, delayed germination and the like of medicago truncatula and other hard-seed leguminous seeds. The key function of the MtGH9-24 gene in regulating physical dormancy of medicago truncatula seeds is disclosed for the first time, important candidate genes and molecular tools are provided for cultivating new medicago truncatula varieties with low dormancy and high germination rate in the future, and important theoretical value and application potential are achieved.

The application discloses a mesquite alfalfa plant type development control protein, a gene coded by the protein and application thereof. The application firstly finds a Tnt1 insertion mutant mini-1 with changed plant type from a mesquite alfalfa Tnt1 insertion mutant library, clones the plant type development control gene MINI through whole genome resequencing combined with flanking sequence analysis, then in order to prove that MINI is the plant type development control gene, a natural mutant mini-2 of the MINI gene is screened in the mutant library, and a MINI gene knockout strain mini-3 is constructed, and it is found through analysis that the mini-2 and mini-3 phenotypes are completely same as the mini-1 mutant. The above results prove that the MINI gene in the application can regulate plant type. The application lays a foundation for the plant type development research of legume plants such as alfalfa and other dicotyledonous plants.

The invention discloses application of an LMA1 gene in a plant to regulation and control of plant leaf anthocyanin synthesis, and belongs to the technical field of plant anthocyanin synthesis regulation and control. The nucleotide sequence of the LMA1 gene is as shown in SEQ ID No.1. Experiments prove that the content of anthocyanin in plant leaves can be increased through LMA1 gene function deletion mutation in plants created through gene editing; the method is applied to a plant containing the LMA1 gene, such as a leguminous plant medicago truncatula, and a gene editing plant with high anthocyanin content can also be obtained through LMA1 gene function deletion mutation in a plant created through gene editing; accumulation of anthocyanin in plant leaves is changed through the LMA1 gene, the adaptive relation between plant growth and environmental pressure is improved, plants actively cope with the environmental pressure, and the nutritional function of the plants is enriched through increase of the anthocyanin content.

The invention discloses application of a calmodulin gene CML17 in regulation and control of salt tolerance of alfalfa plants. Experiments show that the salt tolerance of the medicago plant medicago truncatula can be effectively improved by inhibiting the expression of the gene, otherwise, the salt tolerance is reduced, that is, the environmental adaptability regulation and control of the medicago plant can be effectively realized by regulating and controlling the expression of the calmodulin gene CML17, so that the regulation and control of the yield are realized. Besides, medicago truncatula is selected as a leguminous plant, the research result of medicago truncatula can be transformed into soybean, peanut and other commercial crops through comparative genomics, and a new direction is provided for constructing a salting agricultural ecosystem.

The invention discloses an ABA (abscisic acid) related protein CAR4 gene of a C2 structural domain of alfalfa and application of the ABA related protein CAR4 gene. The cDNA sequence MsCAR4 of the alfalfa C2 structural domain ABA related protein CAR4 gene is cloned from alfalfa, and research finds that after the gene is over-expressed in alfalfa, branches are reduced, flowering is advanced, and the yield is reduced; meanwhile, after the gene is interfered in medicago sativa, branches are increased, flowering is delayed, and the yield is improved. According to the invention, genes for regulating late flowering of medicago sativa and promoting branch growth are excavated and related functional studies are carried out, so that a theoretical foundation can be laid for variety improvement of medicago sativa and breeding of germplasm resources.

The present invention belongs to the technical field of biological genetic engineering, and particularly relates to a method for analyzing the salt stress of an alfalfa gene promoter. The promoter is cloned from the genome of Medicago truncatula variety R108 treated with salt stress through PCR technology. By analyzing the elements of the promoter, it is found that there are multiple cis-acting elements related to adversity stress distributed therein. The present invention constructs a plant expression vector of the reporter gene GUS using the promoter MsPro1, transforms plants through the Agrobacterium infection method, and obtains transgenic Arabidopsis thaliana. Experiments confirm that under salt treatment conditions, the expression activity of the reporter gene GUS in transgenic Arabidopsis thaliana is significantly higher than that of the control, indicating that the promoter MsPro1 is a promoter induced by salt stress. This promoter can be applied to the research of plant salt tolerance genes or salt tolerance gene engineering breeding.