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Method for improving salt stress tolerance of medicago truncatula

A technology for resisting salt damage and alfalfa is applied in the field of utilization of microbial resources and improvement of salinized soil, which can solve the problems of lack of in-depth and systematic research on plant species selection, microbial species selection and bioremediation mechanism, so as to promote the increase of biological yield. , The method is simple, and the effect of migration is reduced

Inactive Publication Date: 2016-08-17
HEBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] At present, there are still few effective cases of the interaction between rhizosphere microorganisms and effective plants for saline-alkali soil improvement at home and abroad, especially in the selection of plant species, microbial species selection and bioremediation mechanism. There is still a lack of in-depth and systematic research.

Method used

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  • Method for improving salt stress tolerance of medicago truncatula
  • Method for improving salt stress tolerance of medicago truncatula
  • Method for improving salt stress tolerance of medicago truncatula

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Utilize piriformis indica to improve the salt tolerance of Medicago truncatula, comprising the following steps:

[0030] (1) Preparation of Pyromorpha indica solid CM medium: dissolve 20g glucose, 2g peptone, 1g yeast extract, 1g hydrolyzed casein, 50ml 20× salt solution and 1ml trace elements in water to 1 liter, add 15g Agar powder, sterilized by high temperature and high pressure, poured into the plate after cooling to become solid CM medium.

[0031] Wherein the preparation method of 20× salt solution is: mix 12g NaNO 3 , 10.4g KCl, 10.4g MgSO 4 ·7H 2 O, 30.4gKH 2 PO 4 Put it in a container and add sterilized water to 1 liter.

[0032] The preparation method of trace elements is: 6g MnCl 2 4H 2 O, 1.5g H 3 BO 3 , 2.65g ZnSO 4 ·7H 2 O, 750mg KI, 2.4mg Na 2 MO 4 ·7H 2 O, 130mg CuSO 4 ·5H 2 O into the container, add sterilized water to 1 liter.

[0033] (2) Activation of strains: Inoculate the Pyromorpha indica strains stored in the refrigerator on the s...

Embodiment 2

[0061] In the embodiment 1, Pyromorpha truncatula is inoculated with Medicago truncatula (step 5) and changed into: the spore suspension of Pyromorpha truncatula is mixed in the FM substratum, and then the Medicago truncatula seeds through surface disinfection are put into containing (experimental group) Germinate in the medium that does not contain (control group) Indian piriformis spore suspension, all the other are equal to embodiment 1. After 35 days, potted Medicago truncatula is 100mM under different salt concentrations, 150mM, 200mM, 250mM, Indian piriformis spores invade Compared with the non-infection group, the root sodium ion content of the infected group decreased by 44.85%, 41.58%, 39.36%, and 36.86%, respectively.

Embodiment 3

[0063] In Example 1, Pyromorpha indica inoculates Medicago truncatula (step 5) into: first carry out surface disinfection treatment to Medicago truncatula seeds, germinate in advance in the petri dish at 25 ℃, after 4-5 days, treat that seed grows 2- The 3cm buds were soaked in the spore suspension of Pyriformis spp. at 25°C for 30 minutes, and the seedlings were transferred to FM medium for conventional cultivation at constant temperature and humidity for 7 days, and then transferred to flower pots for cultivation. All the other are equal to embodiment 1. After 35 days, potted Medicago truncatula 100mM under different salt concentrations, 150mM, 200mM, 250mM, Indian pyriform spore infestation group compares non-infection group root sodium ion content to drop 46.25%, 45.36% respectively, 37.28%, 35.56%.

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Abstract

The invention relates to a method for improving salt stress tolerance of medicago truncatula. The method comprises the steps: (1) preparing a solid CM complete medium for piriformospora indica; (2) culturing the piriformospora indica and collecting a spore suspension; (3) inoculating the piriformospora indica to the medicago truncatula; and (4) transplanting medicago truncatula seedlings and carrying out later-stage management. According to the method, the piriformospora indica spore suspension is adopted to soak seeds in advance, so that the piriformospora indica can be parasitized to surfaces of the medicago truncatula seeds, the seeds are promoted to germinate ahead of time, and the germination percentage of the seeds is remarkably increased (increased to 95.0% from the original 80.0%); the biological yield of the medicago truncatula can be remarkably increased; and through parasitizing the piriformospora indica to roots of the medicago truncatula, the accumulation of sodium ions in the roots of the medicago truncatula can be lowered, and thus the salt stress tolerance of the medicago truncatula is improved.

Description

technical field [0001] The invention relates to the technical field of utilization of microbial resources and improvement of salinized soil, in particular to a method for improving salt damage resistance of Medicago truncatula. Background technique [0002] Salinized land is one of the important land types in my country. With the continuous aggravation of sewage irrigation, improper use of chemical fertilizers and industrial pollution, the area of ​​secondary salinized soil is expanding day by day, and the area of ​​arable land is gradually decreasing. Therefore, it is of great significance to carry out salinization land treatment and soil improvement to deal with the problems of population growth and decrease of cultivated land in China at this stage. The main measures to control soil salinization include engineering measures, physical and chemical remediation, phytoremediation and rhizosphere microbial remediation. The method of combining plants and rhizosphere microorga...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00A01C1/00
Inventor 李亮马朝阳武洪庆齐树亭
Owner HEBEI UNIV OF TECH
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