61results about How to "Increased proline content" patented technology

The invention discloses a method for propagating sulzer braconid flies. The method comprises the following steps: firstly, culturing a host plant of tobaccos, then propagating sulzers and afterwards propagating the sulzer braconid flies by the sulzers. By propagating the flies in batches, the multi-generation repeated propagation of the sulzer braconid flies is avoided, the generation quantity ofthe hyperparasite flies of the sulzer braconid flies is reduced, and the survival capability of the sulzer braconid flies in the field is enhanced. The propagation time and quantity of the sulzer braconid flies can be flexibly regulated and controlled according to the actual condition of the field, and different requirements on the sulzer braconid flies under different situations are met. The emergence rate of the stiff aphids of the sulzer braconid flies propagated by the invention is more than 90%, the male-female ratio is 0.8-1.0:1, and the utilization rate of the stiff aphids or the adultflies is more than 80%.

The invention discloses a sugar-increasing high-yield green planting method for beta vulgaris. The method comprises the following steps: step 1, selecting seeds and performing pretreatment; step 2, preparing a traditional Chinese medicine sterilizing liquid; step 3, sterilizing the seeds through immersing; step 4, preparing a seed coating agent specially used for the beta vulgaris; step 5, treating the seeds of the beta vulgaris with the seed coating agent; step 6, accelerating germination; step 7, raising seedlings; step 8, pretreating planting land; step 9, transplanting the seedlings; step10, performing management in a seedling stage; step 11, performing leaf surface management in the seedling stage; and step 12, performing harvesting. The method provided by the invention significantlyimproves the virus resistant and mildew resistant ability of the seeds, so that the germination energy and the seed vitality are significantly improved, the seeds have a high seedling emergence rateand a quick germination speed, microenvironments for germination and seedling growth are excellent, the yield and the sugar content of the beta vulgaris can be significantly increased, the costs are reduced, the benefits are improved, and the method provides powerful guarantee for large-scale planting, and has higher practical value and good application prospects.

The invention relates to a method for planting lawn plants by combining rare earth cerium with mixed culture matrices. The method comprises the step of sowing seeds of lawn plants on mixed culture matrices for lawn planting, wherein 1.5-2kg of mixed culture matrix soil contains 200-800mg / Kg of rare earth cerium, and the mixed culture matrices refer to soil and municipal solid waste compost in a ratio of parts by weight of 4: (1-2). By applying a certain amount of rare earth cerium, the activities of protective enzymes of the lawn plants can be obviously improved, and the defence capability of the protective enzyme system can be enhanced, thus improving the drought resistance of the lawn plants. Efficient and low-cost operation modes in large-area municipal lawn planting are expected to be achieved through the application of the technology.

The invention discloses a method for long-term storage of Curcuma kwangsiensis 'Ma Nao' seed-balls. The method comprises the steps of (1) treatment of seed-balls: collecting, cleaning and disinfecting and then drying the Curcuma kwangsiensis 'Ma Nao' seed-balls; (2) chemical treatment: performing soaking treatment on the Curcuma kwangsiensis 'Ma Nao' seed-balls with chemicals to improve the anti-rotting capacity of the seed-balls during storage and drying the seed-balls; (3) storage: covering the seed-balls in clean, loose and moist storage matrixes in a refrigerating chamber at the temperature ranging from 7+ / -2 DEG C to 29+ / -2 DEG C and the humidity between 30% and 50%. By the aid of the method, the storage time of the Curcuma kwangsiensis 'Ma Nao' seed-balls can be as long as 10 months, and the seed-ball rotting rate during storage is low, so that flower annual production requirements are met, and early spring cultivation is achieved.

The invention discloses a soybean 90kDa heat shock protein family encoding gene and an application thereof. The soybean 90kDa heat shock protein and an encoding gene GmHSP90 of the soybean 90kDa heat shock protein, disclosed by the invention, comprise 12 members which are respectively placed in cytoplasm, endoplasmic reticulum, chloroplast and mitochondria; plant overexpression vectors 35S-GmHSP90-pEarleyGate103 respectively constructed by five typical genes convert arabidopsis to obtain expressed transgenic arabidopsis as well as mRNA (Messenger Ribose Nucleic Acid) high-level expression; an overexpression transgenic GmHSP90 plant shows higher resistance to heat stress and low-temperature stress, is certainly resistant to both permeation and drought stress, and also has resistance to high-concentration calcium ions. Therefore, such expressed gene can be used as a target gene for being introduced into the plant through overexpression to improve the comprehensive resistance of the transgenic plant.

The invention relates to a method for improving salt stress tolerance of medicago truncatula. The method comprises the steps: (1) preparing a solid CM complete medium for piriformospora indica; (2) culturing the piriformospora indica and collecting a spore suspension; (3) inoculating the piriformospora indica to the medicago truncatula; and (4) transplanting medicago truncatula seedlings and carrying out later-stage management. According to the method, the piriformospora indica spore suspension is adopted to soak seeds in advance, so that the piriformospora indica can be parasitized to surfaces of the medicago truncatula seeds, the seeds are promoted to germinate ahead of time, and the germination percentage of the seeds is remarkably increased (increased to 95.0% from the original 80.0%); the biological yield of the medicago truncatula can be remarkably increased; and through parasitizing the piriformospora indica to roots of the medicago truncatula, the accumulation of sodium ions in the roots of the medicago truncatula can be lowered, and thus the salt stress tolerance of the medicago truncatula is improved.

The invention discloses a plant salt tolerance-related protein IbERD3 as well as an encoding gene and an application of the plant salt tolerance-related protein IbERD3. The protein provided by the invention is from a sweet potato (Ipomoea batatas), is named as IbERD3 and is a protein as shown in (a) or (b), wherein (a) is a protein composed of an amino acid sequence as shown in a sequence 1 in a sequence table, and (b) is a protein formed by substituting and / or deleting and / or adding one or more amino acid residues in the amino acid sequence as shown in the sequence 1, is related to the plant salt tolerance and is derived from the sequence 1. The IbERD3 gene is induced to the sweet potato to obtain an IbERD3 transgenic sweet potato which is remarkably improved in salt tolerance as comparison with a wild sweet potato. The protein IbERD3 and the encoding gene thereof provided by the invention have important application values in improving the salt tolerance of plants. The plant salt tolerance-related protein IbERD3 is wide in application space and market prospect in the agricultural field.

The invention discloses a drought resistant relevant protein IbBT4 and a coding gene and application thereof. The invention discloses the protein firstly. The protein is a protein of which the amino acid sequence is as shown in SEQID NO.1, or a fusion protein obtained through connection of an N end or / and C end of an amino acid sequence as shown in SEQID NO.1 with a protein tag. The invention further discloses the coding gene and the application of the protein. The invention finds the IbBT4 protein and the coding gene thereof, the coding gene of the IbBT4 protein is guided into arabidopsis thaliana, a transgenic arabidopsis thaliana plant overexpressing IbBT4 is obtained, the transgenic plant is subjected to drought stress treatment, the drought resistance is strengthened, and the droughtresistant relevant protein IbBT4 has important effects in a drought resistant process of plants, has important application value in research of increasing drought resistance of the plants, and has wide application space and great market prospects in agriculture field.

The invention provides a preservation method for a butterfly orchid cut-flower, wherein red dragon butterfly orchid is used as the material for researching the physiological effect on the life of a butterfly orchid cut-flower vase caused by treatment with different pretreatment liquid concentrations and different combinations of fresh-keeping solutions. Results show that after being pretreated by silver thiosulfate (STS) and placed in the fresh-keeping solutions, the life the butterfly orchid cut-flower is obviously prolonged, and the fresh weight index of flower branches is obviously improved, wherein the most preferable formula is the STS with the concentration of 2.0mmol / L applied to pretreatment for 1.5h and the fresh-keeping solution consisting of 2% of cane sugar, 200mg / L of 8-HQS (Hydroxyquinoline Sulfate) and 25mg / L of silver nitrate; the fresh-keeping solution is beneficial to prolonging the life of the butterfly orchid cut-flower vase, delaying the reducing speed of fresh weight and SOD (Superoxide Dismutase) activity, and slowing down the rising of proline content; and under the condition, the life of the butterfly orchid cut-flower vase can be prolonged for 26.75 days at most.

The invention discloses an IbAITR5 gene derived from ipomoea batatas, a protein encoded by the IbAITR5 gene and application of the protein. The invention firstly discloses the IbAITR5 gene derived from the ipomoea batatas; and the gene is a DNA molecule as shown in SEQ ID NO. 2 or a DNA molecule with a coding sequence as shown in SEQ ID NO. 2. The invention further discloses the protein coded by the above-mentioned gene and the application of the protein. The IbAITR5 protein and the coding gene thereof are discovered; the coding gene of the IbAITR5 protein is introduced into the ipomoea batatas to obtain transgenic ipomoea batatas plants overexpressing IbAITR5; the transgenic plants are subjected to drought stress treatment, and the drought resistance of the transgenic plants is enhanced;and the IbAITR5 protein and the coding gene thereof have important application value in improvement of drought resistance research of plants, and have wide application space and market prospects in the agricultural field.

The invention discloses sweet potato low-temperature-resistant related protein IbICE1 and coding genes and an application thereof. The sweet potato low-temperature-resistant related protein IbICE1 is one of protein in a), b) and c). The amino acid sequence of the protein a) is the same as that of the protein shown in the sequence 2 of a sequence table; the protein b) is the protein obtained by making the N end and / or the C end of the protein shown in the sequence 2 of the sequence table connected with labels; according to the protein c), one or more amino acid residues are replaced and / or lost and / or added to the amino acid sequence shown in the sequence 2 of the sequence table, and the protein with the same function is obtained. According to experiments, it is verified that the protein IbICE1 and the coding genes thereof play an important role in the process of resisting abiotikus stressz of plants, important application value is achieved in the study of improving the low temperature resistance of the plants, and wide application space and market prospect are achieved in the agriculture field.

The invention provides a CRR2 gene for improving low-temperature tolerance of plants. The nucleotide sequence of the CRR2 gene is shown as SEQ ID NO:1. The invention also provides RNA (Ribonucleic Acid) correspondingly transcribed by the CRR2 gene and application of the RNA. The CRR2 gene and the corresponding transcribed RNA of the CRR2 gene can specifically respond to low-temperature stress response. The subcellular localization analysis shows that the fluorescent probe is localized in cell nucleus and cytoplasm. The CRR2 gene is further transferred into cassava, a cassava CRR2 high-expression strain is obtained, it is found that the CRR2 high-expression strain is more tolerant to low-temperature stress, the survival rate is greatly increased, meanwhile, the proline content in the CRR2 high-expression strain is remarkably increased, the malondialdehyde (MDA) content after low-temperature treatment is remarkably reduced, it is indicated that the CRR2 and the CRR2 gene can improve the low-temperature tolerance of plants, and in the future, the plant is subjected to genetic improvement by using the gene, and an effective resource can be provided for improving the low-temperature tolerance of the plant.

The invention discloses pantoea alhagi and application thereof. The pantoea alhagi is classified and named as Pantoea alhagi, the strain name is XK-11, the pantoea alhagi is preserved in the China General Microbiological Culture Collection Center on March 29th, 2018, and the preservation number is CGMCC NO.15526. In the invention, the exopolysaccharide is derived from fermentation of the pantoea alhagi XK-11, and has the advantages of stable source, low cost, environmental friendliness and the like. According to the invention, the exopolysaccharide can enhance and promote the plant growth andenhance the abiotic stress resistance of crops by promoting the photosynthesis of plants, enhancing the proline content of the plants, reducing the MDA content of the plants and the like.

The invention provides application of methyl jasmonate. The methyl jasmonate is applied to easing the waterflooding damage to shallow root vegetables so as to improve the waterlogging resistance of the shallow root vegetables. The application of the methyl jasmonate reduces the damage to the shallow root vegetables, thus improving the survival rate, guaranteeing the yield of the shallow root vegetables under the waterflooding condition, and increasing the income of farmers.

The invention discloses protein CkWRKY33 and a coding gene and application thereof. The amino acid sequence of the protein CkWRKY33 is shown as SEQID NO:2. Experiments prove that the CkWRKY33 gene istransmitted into arabidopsis thaliana Columbia-0 subtype, so that genetically modified Arabidopsis thaliana is obtained. Compared with the arabidopsis thaliana Columbia-0 subtype, the drought resistance of the genetically modified Arabidopsis thaliana is improved, improvement of the drought resistance reflects that the propanedial content is reduced, the soluble sugar content is increased, the proline content is increased, the peroxidase activity is improved, the rate of water loss is reduced, and the motility rate is increased. Therefore, the protein CkWRKY33 can improve the plant stress resistance. The protein CkWRKY33 has important application value.

The invention provides a gene corresponding to LncRNA. The nucleotide sequence of the gene is shown in SEQ ID NO: 1. The invention also provides a transcribed LncRNA corresponding to the gene and application of the LncRNA. The gene corresponding to the LncRNA and the LncRNA transcribed by the gene can specifically respond to low-temperature stress response and drought stress response. By further transferring the gene into cassava, it is found that a transgenic line is short and small in plant, long and narrow in leaf and more tolerant to low-temperature stress and drought stress, the survival rate is greatly increased, the content of proline in the transgenic line is remarkably increased, the content of malondialdehyde is remarkably reduced after drought or cold stress treatment, and it is shown that the LncRNA and the gene thereof can be used for regulating plant growth and the low-temperature and drought tolerance of plants is improved. In the future, the LncRNA and the gene thereof can be used for carrying out genetic improvement on the plants, and effective resources are provided for promoting plant growth and improving the low-temperature tolerance and drought tolerance of the plants.

The invention belongs to the technical field of identification and screening of low-temperature-resistant maize inbred lines, and particularly relates to an identification and screening method of a seedling-stage low-temperature-resistant maize inbred line. The identification and screening method of the low-temperature-resistant maize inbred line comprises the following steps: carrying out low-temperature stress treatment and normal-temperature treatment on maize seedlings to be detected; detecting indexes of the corn seedlings obtained by the two treatment modes; screening out a low-temperature-resistant corn selfing line according to a detection result, wherein the conditions of the low-temperature stress treatment are as follows: culturing is carried out at 6-8 DEG C for 7 days, the indexes are seedling length, chlorophyll content and proline content. According to the method, the optimal treatment condition for low-temperature identification of the maize inbred line in the seedlingstage is determined; the selfing line with strong seedling stage low temperature resistance has good seedling plant form, obvious increase of proline content and less decrease of chlorophyll content under low temperature stress, can reduce the damage of low temperature to the seedling stage growth of plants, provides a powerful guarantee for the growth and development of the seedling stage, and lays a foundation for the large-scale identification and screening of low temperature resistant corn germplasms.

The invention discloses a method for obtaining a dunaliella salina CHYB gene-modified tobacco. According to the method, firstly, tobacco seeds are dipped in the sodium hypochlorite solution and then are cleaned in sterile water. Secondly, the tobacco seeds are dipped in ethyl alcohol, then flushed in sterile water, inoculated in an Murashige and Skoog medium and cultivated to obtain aseptic seedlings. After that, the construction of a plant expression vector Pcambia3301-chyb and the genetic transformation of agrobacterium tumefaciens are conducted. Finally, the transformation of tobacco is conducted based on the leaf-mediated method. According to the method, the dunaliella salina CHYB gene is successfully transferred into tobacco through the agrobacterium tumefaciens-mediated leaf-mediated process. In this way, obtained transgenic tobacco plants are higher in height, leaf area and chlorophyll content compared with plants in a control group. After the stress treatment in 200 mmol / L NaCl, the chyb-modified transgenic tobacco can still survive and grow. Meanwhile, the relative electrical conductivity of tobacco leaves changes slightly, and the proline content in tobacco leaves increases. The salt tolerance of the chyb-modified transgenic tobacco is improved.

The invention discloses a protein IbTPS (Ipomoea batatas trehalose-6-phosphate synthase) related to salt resistance of sweet potato as well as an encoding gene and an application of the protein. The protein related to the stress tolerance of a plant is the protein as (a) or (b): (a) the protein consisting of amino acid sequences indicated by a sequence 2 in a sequence table; and (b) the protein which is related to the stress tolerance of a plant and derived from (a) by substituting, and / or deleting and / or adding one or more amino acid residual sequences for an amino acid residual sequence of the sequence 2 in the sequence table. According to the enzyme encoded by the IbTPS gene, the expression of the gene can improve the stress tolerance of the plant. The IbTPS protein and the encoding gene thereof have an important value on increasing the content of mycose and studying the stress tolerance of the sweet potato. The protein IbTPS and the encoding gene have wide application space and market prospect in the agriculture field.

The invention relates to a compound regulator for defending freeze injury of wheat in spring and a method. The compound regulator is a solution containing 0.021g / L-0.11g / L of salicylic acid and 0.5g / L-1.0g / L of a surfactant. The compound regulator is uniformly sprayed to wheat leaves before 1-2 days of the freeze injury of the wheat in the spring. By utilizing a compound regulator solution, the resistance of wheat plants on the freeze injury in the spring can be remarkably enhanced, the freeze injury degree of young ears of wheat can be obviously decreased, and the yield of wheat grains can be increased. The compound regulator has a simple formula and easy for popularization and generalization, the raw materials are easily available, and an application method of the compound regulator is simple.

The invention discloses an application of protein AeZDS and encoding gene thereof in promoting accumulation and stress resistance of plant carotenoids. The invention provides a protein (a) or (b) as follows: (a) the protein composed of an amino acid sequence shown as sequence SEQ ID NO.2 in a sequence table, and (b) the protein with one or more substituted and / or deleted and / or added amino acid residue in the amino acid sequence shown as sequence SEQ ID NO.2 in the sequence table. When the encoding gene of protein is introduced into arabidopsis, the content of carotenoid in transgenic arabidopsis is obviously increased and the stress resistance of transgenic arabidopsis plant is promoted. The protein AeZDS and the encoding gene thereof related to biosynthesis and stress resistance of carotenoids have important theoretical significance and practical value in regulating biosynthesis and stress resistance of plant carotenoids.

The invention relates to an application of Leymus chinensis Trin chitinase (LcChi2) gene in improving cold resistance of plants, relating to the field of the plant molecular biology. The application method comprises the following steps: (1) connecting the LcChi2 gene on an expression regulation sequence on a carrier in an operationable manner, so as to form a recombinant vector containing the nucleotide sequence shown in SEQ ID NO:1; (2) transforming host cells of the recombinant vector obtained in the step (1), and screening to obtain transformed cells; and (3) culturing the transformed cells obtained in the step (2) to obtain a LcChi2 gene transfer plant with cold resistance and the offspring of the LcChi2 gene transfer plant. In the step (1), the vector is plant expression vector pBI121 or plant expression vector pCAMBIA3300. The test proves that the seed germination rate of LcChi2 gene transfer tobacco in the cold environment is obviously improved, overexpression LcChi2 genes of dicotyledon tobacco and monocotyledon corn and rice obviously improve the cold resistance of the plants during the seedling stage, so that the result shows that the overexpression LcChi2 gene obviously improves the cold resistance of plants, including tobacco, corns, rice and the like.

The invention discloses a salt resistance related protein IbEST of a sweet potato and a coding gene thereof and application thereof. The protein related to salt resistance of the sweet potato provided by the invention is named IbEST, comes from Ipomoea batatas and is shown in (a) or (b) below: (a) the protein composed of amino acid sequences shown as a sequence 2 in a sequence list; and (b) the protein derived by the sequence 2 by replacing, deleting and / or adding residual acids of one or multiple amino acids in the amino acid sequence as shown by the sequence 2 in the sequence list and related to the stress tolerance of a plant. The experiment proves that the IbEST gene and the coding protein play an important role in the process of withstanding abiotic stress by the plant. The IbEST protein and coding gene provided by the invention have an important application value in the research of improving the salt tolerance of the plant. The salt resistance related protein IbEST of the sweet potato and coding gene have wide application space and market foreground in the agricultural field.

The invention discloses a protein IbEGF and a related biological material and application thereof. The invention firstly discloses a protein which is a protein with an amino acid sequence shown as SEQID NO.1 or a fusion protein obtained by connecting an N terminal or / and a C terminal of the amino acid sequence shown as SEQ ID NO.1 with a protein tag. The invention further discloses a biological material related to the protein and application. The IbEGF protein and the coding gene thereof are discovered, the coding gene of the IbEGF protein is introduced into sweet potatoes to obtain transgenic sweet potato plants over-expressing IbEGF, the transgenic plants are subjected to drought stress treatment, and then the drought resistance of the transgenic lines is enhanced. Therefore, the IbEGFprotein and the coding gene thereof have important application value in the research of improving drought resistance of plants.

The invention discloses application of a protein OsZZW1 in regulating and controlling drought resistance of oryza sativa. The amino acid sequence of the protein OsZZW1 is shown in SEQ ID NO:2. Experiments show that the drought resistance of oryza sativa can be improved by reducing expression of the protein OsZZW1 in Nipponbare; and improvement of the drought resistance lies in that the survival rate is increased, the content of proline is increased, and the content of malonaldehyde is reduced. The protein OsZZW1 is capable of regulating and controlling drought resistance of oryza sativa. The application has significant application value.

The invention discloses a poplar latex protein MLP gene capable of improving the cadmium stress resistance of a plant and an expressed protein and application thereof, and relates to a gene capable of improving the cadmium stress resistance of the plant and an expressed protein and application thereof. The nucleotide sequence of the poplar latex protein MLP gene capable of improving the cadmium stress resistance of the plant is shown as SEQ ID NO: 1. The amino acid sequence of the poplar latex protein MLP expressed by the gene is shown as SEQ ID NO: 2. The gene is used for improving the cadmium stress resistance of plants. The MLP gene of the transgenic MLP gene plant is overexpressed under the condition of CdCl2 stress, so that the Cd < 2 + > absorption of the transgenic plant is reduced, the POD enzyme activity in an active oxygen scavenging system is increased, and the content of an osmotic regulation substance proline is increased, thereby reducing the damage of CdCl2 stress to the plant.

The invention provides a bioactive preparation. The bioactive preparation is prepared from the following components in parts by mass: 20 to 30 parts of biochar, 40 to 50 parts of medical stone, 10 to20 parts of humate and 5 to 10 parts of polyacrylamide. The preparation method of the bioactive preparation comprises the following steps: carrying out anoxic pyrolysis on household garbage in a pyrolysis furnace at 300-600 DEG C for 1-2 hours, crushing the obtained biochar, medical stone, humate and polyacrylamide to 200 meshes, uniformly mixing according to a ratio, adding 15-20% of water, and carrying out extrusion granulation to obtain the bioactive preparation. The bioactive preparation provided by the invention can reduce the heavy metal Cr (VI) water-soluble state, exchangeable state and carbonate binding state of calcareous soil, can increase the organic binding state and residue state, and promote the conversion of the heavy metal Cr (VI) from an effective state to a slow / delayedstate. Meanwhile, the granular structure is easy to use; compared with the prior art, the adsorbing and fixing effect on Cr (VI) is remarkable, and good application and popularization prospects are achieved.