45 results about "Piriformospora" patented technology

The invention discloses a method for producing a biological bacterial fertilizer by taking waste substances as a raw material, belongs to the technical field of biological bacterial fertilizers, and aims to develop a biological bacterial fertilizer which has the effects of resisting cold, drought, waterlogging and diseases and increasing yield by decomposing and fermenting paper mill garbage and urban domestic garbage through the specific functions of a piriformospora indica bacterial strain and bacillus subtilis serving as natural plant growth regulating agents. The technical route comprises the following steps: treating waste rice hull produced in a paper mill and cellulose substances such as straw, and preparing a culture medium together with leftover dry powder; inoculating a fermentation liquor mixture of cellulose degradation bacteria, such as trichoderma, bacillus megaterium, potassium bacteria and the piriformospora indica bacterial strain; fermenting to obtain the liquid bacterial fertilizer. By adopting the method, the current three problems of effective utilization of recyclable resources such as straw and rice hull, pollution abatement of paper mills and urban domestic garbage disposal are solved; meanwhile, the biological bacterial fertilizer has the advantages of improvement on the yield and quality of various crops, low production cost, remarkable fertilizer effect, improvement on the stress resistance of the crops, and very remarkable economic benefit.

Provided is a method for reducing the cadmium content in tobacco leaves. The method comprises the specific steps that (1) nanometer material zinc oxide is used for replacing ZnSO4.7H2O in original Kafer culture media, and 10 mg of nanometer material zinc oxide is added into 1 liter of culture media; (2) activated Piriformospora indica is inoculated to the liquid Kafer culture media in the step (1) for culture, then mycelia are collected, Piriformospora indica mycelium liquid is made and poured to roots of tobacco seedlings, and root-irrigation is conducted so that the Piriformospora indica can be colonized to the roots of the tobacco seedlings; (3) the tobacco seedlings are transplanted after the Piriformospora indica is colonized, and conventional water and fertilizer management are conducted so that the tobacco seedlings can grow normally. The growth status of the tobacco seedlings can be improved in the method, the amount of cadmium moving to overground parts of the tobacco seedlings from root systems is reduced, heavy metal residue on the tobacco leaves is reduced, and the method is easy to master, low in cost, simple, practical and environmentally friendly, and can not case secondary pollution.

The invention provides a method for promoting rooting of tissue-cultured transplant seedlings of Gerbera jamesonii; irrigating with Piriformospora indica is performed, and Piriformospora indica is used to promote rooting of the tissue-cultured transplant seedlings of Gerbera jamesonii, improve the roots and promote the growth of plants; in addition, the method may also solve the problems of regenerated plant root weakness, low growth speed, seedling weakness and the like due to multiple subculture, and is significant to promoting the commodity value of Piriformospora indica plants. The method has simple steps, is highly operable and has significant effect.

The invention discloses a method for lowering the content of chromium in tobacco. According to the method for lowering the content of chromium in the tobacco, piriformospora indica is grown on the root of the tobacco in a parasitic manner so as to reduce heavy metal chromium residuals in the tobacco. The method for lowering the content of chromium in the tobacco specifically comprises the steps that (1) ZnSO4 7 H2O in an original Kafer culture medium is replaced by nanometer material zinc oxide, and 10 mg of nanometer material zinc oxide is added to each liter of culture medium; (2) activated Piriformospora indica is inoculated to the Kafer culture medium obtained in the step (1) to be cultured, then mycelia are collected, piriformospora indica hypha liquid is prepared, the prepared piriformospora indica hypha liquid is then poured to the root of a tobacco seedling, and root-irrigation treatment is conducted, so that the piriformospora indica is colonized on the root of the tobacco seedling; (3) after the piriformospora indica is colonized on the root of the tobacco seedling, the tobacco seedling is transplanted to a land, and then conventional water and fertilizer management is conducted to make the tobacco seedling grow normally. According to the method for lowering the content of chromium in the tobacco, the growth condition of the tobacco can be improved, the amount of heavy metal chromium which transfers from the root of the tobacco to the overground part of the tobacco is reduced, the heavy metal chromium residuals in the tobacco are reduced, the method is easy to master, the cost is low, the method is easy and practical, environmental friendliness is achieved, and secondary pollution can not be caused.

The invention discloses a method of using joint action of medicago sativa and piriformospora indica to restore petroleum-contaminated soil. The method includes following steps: (1), culturing piriformospora indica, and collecting spore suspension; (2), cultivating seeds of medicago sativa; (3), inoculating piriformospora indica to medicago sativa. The piriformospora indica spore suspension is adopted to irrigate roots of medicago sativa, piriformospora indica is planted at the roots of medicago sativa, mycorrhizal symbionts formed by plants and endophytic fungi can promote growing of medicago sativa, bioactivity and fertility of soil are improved, petroleum in the soil can be effectively degraded, and degradation rate can reach 78%.

The invention provides a method for reducing cadmium content of an overground part of paddy rice. The method comprises the following specific steps: (1) by use of utilizing nano material ZnO to replace ZnSO4.7H2O in an original Kafer culture medium, adding 10mg of nano material ZnO into per litre of the culture medium; (2) inoculating the activated piriformospora indica into the liquid Kafer culture medium obtained in the step (1) for culturing, then collecting mycelia, preparing a piriformospora indica mycelium solution, then pouring the piriformospora indica mycelium solution to the root of a paddy rice seedling, and performing root-irrigation treatment so as to colonize the piriformospora indica at the root of the paddy rice seedling; (3) after performing piriformospora indica colonization, transplanting the paddy rice into a large field, and then performing routine water and fertilizer management so as to enable the paddy rice to grow normally. The method is easy to master, relatively low in cost, simple and practical, environment-friendly, incapable of causing secondary pollution, applicable to production of chemical-free rice, not only applicable to planting of rice in heavy metal contaminated soil, and also applicable to uncontaminated soil.

The invention discloses a method for utilizing piriformospora indica and Zhongshengmycin to jointly prevent and control bacterial wilt of tobaccos. The method includes the following steps that 1, tobacco seeds are sowed in a seedling raising substrate mixed with piriformospora indica hyphae, and seedlings are transplanted to fields after seedling development; 2, before transplanting, holes for tobacco seedling transplanting are irrigated with a Zhongshengmycin solution; root-irrigation treatment is conducted on tobacco plants by using the Zhongshengmycin solution. The piriformospora indica which is adopted by the method and is not inhibited by the Zhongshengmycin can combine with the fungicide Zhongshengmycin for usage and is applied to prevention control of the bacterial wilt of the tobaccos. Compared with the prevention and control effect of the separately used piriformospora indica or the Zhongshengmycin, the jointly used piriformospora indica and Zhongshengmycin can play a synergic effect and remarkably improve the prevention and control effect on the bacterial wilt of the tobaccos.

The invention discloses a piriformospora indica fermentation method. The method comprises the following steps: by taking a PDB culture medium as a culture solution, regulating the pH of the culture solution to be 5.0-5.1 by using a HCl solution with the concentration of 1mol/L, and performing culture fermentation on piriformospora indica at the temperature of 27.5-28.5 DEG C for 14-15 days, so as to obtain the fermentation liquor. The invention also discloses a method for performing response surface optimization in the piriformospora indica fermentation process. The method comprises the following steps: setting the three variables including the pH, the concentration of the PDB culture medium and the culture time; optimizing according to the Box-Behnken design principle by utilizing design expert software. According to the method, the yield of secondary metabolites can be increased, and the consumption of nutritional ingredients and culture media is reduced.

The invention provides a method for promoting rooting and root strengthening of banana tissue culture seedlings. The method comprises the following steps: cultivation of Piriformospora indica, preparation of a banana rooting medium, inoculation for co-culture of Piriformospora indica and banana tissue culture seedlings, and the like. Colonization of Piriformospora indica is used for promoting rooting of banana tissue culture seedlings, the method has simple operation, rooting period of tissue culture seedlings is shortened, and the method can substantially promote rooting of the banana tissue culture seedlings with a certain root strengthening effect. The method has good operationality and substantial effects.

The invention discloses an application of Piriformospora indica to improvement of the resistance of plants to bensulfuron methyl serving as a herbicide and belongs to the technical field of crop planting. The Piriformospora indica is not inhibited by bensulfuron methyl, and the established Piriformospora indica-crop co-culture system is capable of remarkably improving the resistance of crops to bensulfuron methyl and reducing phytotoxicity; and after the Piriformospora indica serving as a beneficial endophytic fungus is colonized into the crops, no side effects are generated, and meanwhile, the environment safety is guaranteed. The crops are rice, rapes, sunflowers, tobaccos, wheat and maize. The invention further provides a method for improving the resistance of rice to bensulfuron methylserving as a herbicide. The method comprises the steps: co-culturing rice seedlings in a rice culture solution containing Piriformospora indica to obtain rice seedlings in which the Piriformospora indica is colonized, and then, transplanting the rice seedlings in which the Piriformospora indica is colonized to a farmland, and culturing the rice seedlings until harvesting rice.

The invention relates to nutrient solution for hydroponics plants capable of releasing negative ions, and belongs to the field of functional plant hydroponics.The negative ions can be continuously released by the hydroponics plants such as tequila by the aid of the nutrient solution, and the lives of the hydroponics plants can be prolonged by the aid of the nutrient solution.The nutrient solution is improved Hoagland nutrient solution with piriformospora indica fungi.The nutrient solution has the advantages that the hydroponics plants (including the tequila and yucca gloriosa) can be cultivated by the aid of soilless cultivation methods, the nutrient solution can be applied to the hydroponics plants on plant negative ion machines, accordingly, the negative ions can be continuously and efficiently released, growth of the root systems of the hydroponics plants can be promoted, the leaves of the hydroponics plants keep deeply green, the negative ions can be assuredly continuously and efficiently released by the hydroponics plants in the plant negative ion machines, and the lives of the plants can be prolonged.

The invention discloses a piriformospora indica microbial inoculum and a preparation method thereof, the piriformospora indica microbial inoculum comprises a fermentation carrier containing piriformospora indica thalli as an active ingredient, a spore protective agent, a dispersing agent and a spreading agent, the content of piriformospora indica chlamydospore in the fermentation carrier is (1.0-20.0) * 10 < 6 > spores/g, the weight percentage of the dispersing agent and the spreading agent is 1-5%, and the weight percentage of the spreading agent is 1-5%. The weight percentage of the spore protective agent is 0.05 to 0.1 percent. The method successfully solves the problem that piriformospora indica is difficult in spore production, large-scale application and long-term storage, when the seed solution is prepared, soluble starch is adopted as a carbon source, compared with other carbon sources, the growth speed of piriformospora indica hyphae can be remarkably increased, the spore production time is remarkably shortened, the spore production quantity is increased, and the production cost is reduced. Through solid culture with sorghum and cane sugar as fermentation carriers, the storability is improved, the prepared microbial inoculum can be preserved for a long time, the spore activity can be kept, and possibility is provided for large-area production and application.

The invention discloses a method for improving yield of liquid fermentation products of Piriformospora indica. The method comprises the following steps: a Piriformospora indica liquid seed solution isinoculated to a solid culture medium with added exogenous substances for culture, and after vacuum freeze drying and pulverizing are carried out for cultivated products, an exogenous substance solidfermentation culture is obtained; exogenous substances are selected from one or two kinds of fresh rehmannia roots and gardenia fruits; the Piriformospora indica liquid seed solution is inoculated toa liquid fermentation culture medium with the added exogenous substance solid fermentation culture, cultivation is carried out for a period of time, cold shock proteins are added, cultivation is continued for a period of time, and after fermentation is terminated, the fermentation products of Piriformospora indica are obtained. The method can be used for effectively promoting generation of Piriformospora indica metabolites.

The invention belongs to the technical field of seedling culture of longan, and particularly relates to a method for increasing germinating rate of longan seeds under condition of short-time room temperature storage. The method is characterized in that the longan seeds under the condition of short-time room temperature storage are soaked into a piriformospora indica fermenting liquid, and are fetched out for seeding; compared with the longan seeds which are not soaked into the piriformospora indica fermenting liquid, the germinating rate is obviously increased, and the seedling can well grow.The method has the advantages that the preparation of the piriformospora indica fermenting liquid is easy, the feasibility is strong, the operation is simple in the seed treatment process, and the higher application and economic value is realized.

The invention discloses an application of piriformospora indica in regulating drought tolerance of osmanthus fragrans. That is, in a cultivation process, the piriformospora indica or a biological agent containing the piriformospora indica is inoculated in osmanthus fragrans seedlings, so that the piriformospora indica and osmanthus fragrans root systems are symbiotic, the drought tolerance of theosmanthus fragrans to soil drought can be stimulated, and the drought tolerance of the osmanthus fragrans is remarkably improved. The normal growth increment can be still maintained under the water supply condition that the maximum field water-holding capacity of the osmanthus fragrans is 15%, meanwhile, the content of soluble sugar and free proline and the activity of POD and SOD enzymes in osmanthus fragrans leaves are remarkably improved, and the content of malondialdehyde is remarkably reduced. Therefore, a new thought and a technical basis are provided for water-saving cultivation of osmanthus fragrans. The piriformospora indica can be rapidly proliferated by utilizing an artificial culture medium, is easy to produce in batches, is further popularized and applied on a large scale, andhas commercial potential advantages.

The invention provides a method for improving the root rot resistance of Gerbera jamesonii. Gerbera jamesonii colonized with Piriformospora indica is obtained by a root immersion technology to improvethe root rot resistance of Gerbera jamesonii, so the method is of great significance to improve the commercial values of Gerbera jamesonii plants. The method has the advantages of simple steps, strong operability and remarkable effect.

The invention belongs to the technical field of planting, and specifically relates to a preparation method for a plant aphid resistance inducing preparation. The preparation method comprises the following steps: inoculating piriformospora indica into a culture medium, and carrying out culturing so as to obtain a piriformospora indica fermentation product; uniformly mixing 20 to 70 parts of the piriformospora indica fermentation product, 20 to 50 parts of jasmonic acid, 10 to 30 parts of oligosaccharide-chain protein, 5 to 8 parts of radix sophorae flavescentis and 60 to 80 parts of a filling agent so as to obtain a mixture; and drying the mixture, and carrying out granulating so as to obtain the plant aphid resistance inducing preparation. The invention also provides the plant aphid resistance inducing preparation. The preparation provided by the invention is simple in preparation steps, environmentally-friendly, free of any pollution and residues, free of side effects, capable of reducing the environmental pollution and overcoming the occurrence of soil-borne diseases, free of the limitation of planting varieties, and wide in application.

The invention provides a method for direct sowing and seedling raising of pleione bulbocodioides by utilizing symbiotic bacteria, which comprises the following steps: mixing piriformospora indica into a culture medium, sowing pleione bulbocodioides seeds, and performing symbiotic culture to obtain symbiotic pleione bulbocodioides seedlings. The method is simple and convenient to operate, the symbiotic bacteria and pleione bulbocodioides seeds are directly sown for seedling raising for the first time, after 10-15 days, most of the seeds appear green, after 30 days, a large number of protocorms appear native meristem, after 45 days, the protocorms grow first leaves, after 2 months, seedlings form 2-3 leaves and grow roots, and the seedlings can be transplanted to a nursery garden.

The invention relates to a method for improving sporulation of piriformospora indica. According to a piriformospora indica culture method, an adopted fermentation culture medium is composed of or prepared from dried potato powder, glucose and water, the content of the dried potato powder in the fermentation culture medium is 10g/L-20g/L, and the content of the glucose in the fermentation culture medium is 10g/L-20g/L. According to the method, piriformospora indica is cultured in the fermentation culture medium composed of the dried potato powder, the glucose and the water, the culture period can be shortened, the spore yield can be increased, the production cost is greatly reduced, and industrial production is facilitated.

The invention discloses a method for controlling tobacco bacterial wilt through combination of Piriformospora indica with trichloroisocyanuric acid. The method comprises steps as follows: (1) sowing tobacco seeds in a seedling culture medium mixed with Piriformospora indica mycelia, and seedlings are transplanted to a field; (2) before transplantation, watering holes for tobacco seedling transplantation with a trichloroisocyanuric acid solution, and 10-15 days after transplantation, performing root-irrigation treatment with the trichloroisocyanuric acid solution. The used Piriformospora indica is not inhibited by the pesticide trichloroisocyanuric acid, can be combined with the trichloroisocyanuric acid for use and is applied to control on the tobacco bacterial wilt. The Piriformospora indica and the trichloroisocyanuric acid are combined for use to play a synergistic role in controlling the bacterial wilt, and the control effect is significantly superior to that of the single Piriformospora indica or the single trichloroisocyanuric acid.