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Nano-zinc oxide-based culture medium capable of promoting microbial growth

A technology of nano-zinc oxide and culture medium, applied in the direction of microorganism-based methods, microorganisms, adding compounds to stimulate growth, etc., can solve the problems of slow growth of Pyromorpha indica, achieve low cost, simple and easy method, good economical effect

Inactive Publication Date: 2014-07-02
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, researchers with research teams from India, Germany, China, Japan, and the United States are committed to studying its function and mechanism of action. The classic medium for cultivating Piriformis indica is Kafer medium, but Piriformis indica is here The growth rate on the culture medium is relatively slow. In order to maintain the characteristics of the original culture medium, promote the rapid growth of Piriformis indica, and improve the effect of metabolites, we invented a method that can increase the growth rate of Piriformis indica and improve the metabolism. The culture medium of biological action has extensive research and application prospects

Method used

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  • Nano-zinc oxide-based culture medium capable of promoting microbial growth
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Experimental program
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Effect test

Embodiment 1

[0021] The culture medium based on nano-zinc oxide of the present invention to promote the growth of Piroiformis indica contains per liter: 2.00g peptone, 1.00g yeast extract, 1.00g hydrolyzed casein, 0.60gNaNO 3 , 0.52gKCl, 0.52gMgSO 4 ·7H 2 O, 1.52gKH 2 PO 4 , 5.00mgZnO, 10mgH 3 BO 3 , 5mgMnSO 4 ·H 2 O, 1.6mgCoCl·5H 2 O, 1.6mgCuSO 4 ·5H 2 O, 1.1mg (NH 4 ) 6 MO 7 o 24 , 1.3mgFeSO 4 , 1.8mg EDTA, 0.10mg thiamine, 0.04mg glycine, 0.01mg niacin, 0.01mg pyridoxine.

[0022] Among them, the particle size of ZnO is 30 nm.

[0023] The method for cultivating Piroiformis indica using the culture medium of the present invention: the culture conditions are 25° C. to 28° C., 100 rpm to 120 rpm shaker culture in the dark.

Embodiment 2

[0025] The culture medium based on nano-zinc oxide of the present invention to promote the growth of Piroiformis indica contains per liter: 2.00g peptone, 1.00g yeast extract, 1.00g hydrolyzed casein, 0.60gNaNO 3 , 0.52gKCl, 0.52gMgSO 4 ·7H 2 O, 1.52gKH 2 PO 4 , 10.00mgZnO, 10mgH 3 BO 3 , 5mgMnSO 4 ·H 2 O, 1.6mgCoCl·5H 2 O, 1.6mgCuSO 4 ·5H 2 O, 1.1mg (NH 4 ) 6 MO 7 o 24 , 1.3mgFeSO 4 , 1.8mg EDTA, 0.10mg thiamine, 0.04mg glycine, 0.01mg niacin, 0.01mg pyridoxine.

[0026] Among them, the particle size of ZnO is 30 nm.

[0027] The method for cultivating Piroiformis indica using the culture medium of the present invention: the culture conditions are 25° C. to 28° C., 100 rpm to 120 rpm shaker culture in the dark.

Embodiment 3

[0029] The culture medium based on nano-zinc oxide of the present invention to promote the growth of Piroiformis indica contains per liter: 2.00g peptone, 1.00g yeast extract, 1.00g hydrolyzed casein, 0.60gNaNO 3 , 0.52gKCl, 0.52gMgSO 4 ·7H 2 O, 1.52gKH 2 PO 4 , 8.00mgZnO, 10mgH 3 BO 3 , 5mgMnSO 4 ·H 2 O, 1.6mgCoCl·5H 2 O, 1.6mgCuSO4·5H2O, 1.1mg(NH 4 ) 6 MO 7 o 24 , 1.3mgFeSO 4, 1.8mg EDTA, 0.10mg thiamine, 0.04mg glycine, 0.01mg niacin, 0.01mg pyridoxine.

[0030] Among them, the particle diameter of ZnO is 30 nm.

[0031] The method for cultivating Piroiformis indica using the culture medium of the present invention: the culture conditions are 25° C. to 28° C., 100 rpm to 120 rpm shaker culture in the dark.

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Abstract

The invention discloses a nano-zinc oxide-based culture medium capable of promoting growth of piriformospora indica. Every liter of culture medium comprises 2.00g of peptone, 1.00g of yeast extract, 1.00g of casein hydrolysate, 0.60g of NaNO3, 0.52g of KCl, 0.52g of MgSO4.7H2O, 1.52g of KH2PO4, 5.00-10.00mg of ZnO, 10mg of H3BO3, 5mg of MnSO4.H2O, 1.6mg of CoCl.5H2O, 1.6mg of CuSO4.5H2O, 1.1mg of (NH4) 6MO7O24, 1.3mg of FeSO4, 1.8mg of ethylene diamine tetraacetic acid (EDTA), 0.10mg of thiamine, 0.04mg of glycine, 0.01mg of niacin and 0.01mg of pyridoxine. Compared with the original culture medium, the nano-zinc oxide-based culture medium has the advantage that the growth speed of the piriformospora indica is obviously faster; the fresh weight of thallus cultured by using the medium is respectively increased by 36.5% and 55.8% compared with the fresh weights of control groups; the dry weight of the thallus cultured by using the medium is respectively increased by 19% and 26.2% compared with the dry weights of control groups; and the bacterial colony is more compact and smoother than the original bacterial colony. The method provided by the invention is simple, uses easily available materials and is low in cost and good in economic benefit.

Description

technical field [0001] The invention relates to the technical field of microorganism culture, in particular to a medium for promoting the growth of Piromorpha indica based on nanometer zinc oxide. Background technique [0002] The nutrients needed for the growth and reproduction of microorganisms mainly include water, carbon sources, nitrogen sources, inorganic salts and growth factors, etc. Microorganisms need many inorganic salts, mainly including P, S, K, Na, Ca, Mg, Fe, etc. Its main function is to form bacterial components, regulate osmotic pressure, act as a component of certain enzymes, and activate enzyme activity. Every microorganism used in industry, agriculture, light industry and medical research and production needs to be produced on a suitable medium. Therefore, it is an important task in the field of microbial research to invent and explore a medium that is conducive to the rapid growth of microorganisms. [0003] Piriformosporaindia (Piriformosporaindia) is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/14C12R1/645
Inventor 楼兵干刘晓曦郭辰彤高其康林福呈
Owner ZHEJIANG UNIV
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