323results about How to "Improve anti-bacterial ability" patented technology

The invention discloses an enterococcus faecium and application thereof. An enterococcus faecium strain provided by the invention is enterococcus faecium suppressant 80, and the collection number is CGMCC No.5058. As proved by experiments, the enterococcus faecium suppressant 80 is obtained by separating, identifying and screening; and the enterococcus faecium suppressant 80 has high resistance, high bacterial resistance and probiotic characteristics, and can be taken as an additive for preparing animal feeds; and the animals include but is not limited to various animals such as pigs, cows, sheep, chicken and the like. The feeds have similar functions to antibiotic feeds, but do not have the side effects of the antibiotic feeds.

The invention discloses an efficient multifunctional seaweed biological fertilizer and relates to the technical field of compound fertilizers. The raw materials include 10-80 parts of seaweed fermentation liquor, 5-30 parts of humic acid, 5-30 parts of urea, 5-20 parts of monoammonium phosphate, 5-20 parts of potassium sulfate and 2-10 parts of trace elements, and all the raw materials are mixed, concentrated and dried at low temperature to obtain the efficient multifunctional seaweed biological fertilizer. The efficient multifunctional seaweed biological fertilizer can quicken photosynthesis of crops, enhance disease resistance and drought resistance, improve crop yield and improve soil structure and soil water and nutrient retention capability, and is worth popularizing energetically.

The present invention provides a production process of fermented and concentrated feed for animal, and the feed is produced with soybean dregs, peanut dregs, corn protein powder, calcium biphosphate, salt, trace elements and leaven in certain weight proportion. The production process includes the steps of: activating and cloning spawn to prepare spawn liquid; mixing the spawn liquid and the said materials to ferment for 15-30 days. The corresponding production apparatus is also provided. The fermented and concentrated feed has increased lactic acid bacteria, saccharomycetes, enzymes, coarse protein, amino acid, etc. and can result in excellent animal feeding effect.

The invention discloses a bacillus licheniformis and application of the bacillus licheniformis. The bacillus licheniformis provided by the invention is concretely bacillus licheniformis C30-2 with the preservation number being CGMCC (China General Microbiological Center Culture Collection Center) No.5350 in CGMCC. Experiments prove that the bacillus licheniformis C30-2 CGMCC No.5350 provided by the invention has strong stress resistance and strong sundry fungus resistance capability. The bacillus licheniformis C30-2 CGMCC No.5350 is emulsified for preparing the bacillus licheniformis preparation, the bacillus licheniformis preparation can be used as additives used for preparing animal feed and can replace antibiotics in animal daily ration for regulating the microecological balance in the animal intestinal tracts, so the effect of enhancing the nonspecific immunity function for realizing the effect of preventing the diseases is realized, and meanwhile, nutrition factors can also be provided for promoting the digestion absorption of nutrition substances, reducing the diarrhea, promoting the animal growth and improving the animal feed conversion rate.

The invention discloses aspergillus niger HC306 and an application of aspergillus niger HC306 to prepare naringenin through naringin conversion. The aspergillus niger HC306 is preserved at Guangdong Microbiology Culture Center and is provided with the accession number of GDMCC No: 60026. The preservation date is Mar. 21<st>, 2016, and the preservation address is the fifth floor, No. 59 building, No. 100 courtyard, Xianliezhonglu, Guangzhou, with the zip code being 510075. The aspergillus niger HC306 is free of toxicity or harmlessness, is safe to use, fast in growth, and good in microbial resistance, and is stable in batches. A fermentation matrix is simple in ingredient and low in market price, and therefore the production cost of naringinase is relatively low. A crude enzyme liquid containing naringinase can be straight applied to the preparation of phloretin, thereby avoiding the separation and purification step of an enzyme and assisting in the reduction of the production cost. The conversion yield of naringenin is high and can reach as high as 93.4%. The preparation method of aspergillus niger HC306 has advantages of fewer by-products and easy extraction of products.

The invention discloses a method for ecologically culturing Rana spinosa, which comprises the following steps of culturing farm construction, culturing time, gutter way water level, culturing density, bait feeding, temperature control and disease prevention. The constructed culturing farm is basically similar to the natural ecological environment and ensures that Rana spinosa can have a good playing and activity space, the metabolism of the Rana spinosa is promoted and the Rana spinosa grow well. The gutter way water level is 2-3cm above the surface of the gutter way, so that the Rana spinosa can have moist skin and strong disease resistance. Baits are more economical and more balanced in nutrition, and the quantity of chemical drug residues in the Rana spinosa is relatively low. The method for ecologically culturing Rana spinosa has the advantages that the culturing farm is basically similar to the natural ecological environment, the playing activity space of the Rana spinosa is proper, germs are not easy to propagate, and the Rana spinosa has balanced nutrition, better growth, strong germ resistance and high survival rate.

The invention relates to a method for preparing fuel of ethanol from immobilized mixed strain fermented cellulosic hydrolysate, which is characterized by utilizing mixed strains of P. tannophilus and S. cerevisilae to graft into diluted acid hydrolysate and fermenting the mixed solution by a supplemented material batch fermentation mode. The method is realized by the following steps: (1) domestication of the P. tannophilus and the S. cerevisilae; (2) immobilization of the mixed strains by calcium alginate; (3) multiplication culture of the immobilized mixed strains; and (4) supplemented material batch fermentation of the immobilized mixed strains. The immobilized mixed strains can be fermented while using xylems and glucose in the hydrolysate for fermentation, thereby the fermentation period is shortened, the utilization ratio of the xylose and the glucose is improved and the ethanol concentration is increased; the immobilized mixed strains can be repeatedly used and have strong anti-hybrid bacteria capability; the supplemented material batch fermentation mode is applied to hydrolysate with cellulose raw material for producing the fuel of ethanol, thereby reducing inhibiting factors in the hydrolysate; and the method is beneficial to thoroughly eliminating the inhibition on substrates, diluting poisonous metabolite, reducing contaminated bacterial, controlling the fermentation time and preventing the generation of side products.

The invention discloses a saline-alkali soil improving agent and a preparation method thereof. The saline-alkali soil improving agent comprises the following components in parts by weight: 8 to 12 parts of desulfurized gypsum, 30 to 50 parts of biogas residue, 20 to 30 parts of sepiolite ore powder, 10 to 15 parts of oyster shell powder, 5 to 10 parts of phosphate, 3 to 8 parts of humic acid ore powder, 2 to 6 parts of expanded vermiculite powder, 1 to 5 parts of ferrous sulfate, 2 to 4 parts of modified plant straw powder, 0.5 to 1.5 parts of diatomite, 0.4 to 0.8 part of carboxymethyl chitosan, 0.3 to 0.7 part of rotten vinasse, 0.2 to 0.6 part of a microbial preparation and 3 to 7 parts of an organic fermented fertilizer. The saline-alkali soil improving agent can effectively improve the soil structure and reduce the alkalization degree of the soil so as to promote the growth of crops and increase production.

The invention discloses a temperature-resistant salt-resistant quick-dissolving thickening agent used for a seawater-based fracturing fluid and a preparation method thereof. The temperature-resistant salt-resistant quick-dissolving thickening agent comprises the following components in percentages by mass: 6% to 8% of acrylic acid, 5% to 6% of sodium hydroxide, 18% to 23% of acrylamide, 1% to 2% of an isolation monomer, 3% to 4% of a functional anion monomer, 3% to 5% of a functional cationic monomer, 4% to 6% of an association intermediate, and 0.4% to 0.8% of an ammonium persulfate-sodium bisulfite redox system or 0.6% to 1.0% of azobisisobutyronitrile which is used as an initiator, with the balance being deionized water. The preparation method for the temperature-resistant salt-resistant quick-dissolving thickening agent comprises the following steps: 1, synthesis of the association intermediate; 2, synthesis of a thickener colloid; and 3, colloid postprocessing. The thickening agent provided by the invention has the following advantages: salt resistance is excellent; the degree of mineralization of a solution has little influence on the rate of swelling; the viscosity-increasing capacity in seawater is strong; antibacterial stability of the solution is strong; the solution has excellent temperature resistance and shearing resistance; and the content of an insoluble substance is extremely small and can not be measured with a conventional method, so the damage of residues on reservoir strata is almost eliminated.

The invention specifically relates to the technical field of a saline alkali soil conditioner and a preparation method thereof. The saline alkali soil conditioner provided by the invention is prepared by mixing the following raw materials according to a certain ratio: bentonite, humic acid, gypsum, sulfur powder, chitosan, zinc sulfate and sodium naphthaleneacetate. After acting on soil of saline alkali land, the saline alkali soil conditioner can obviously reduce the soil acidity or alkalinity, total salt content and volume weight, thus accelerating the formation of soil aggregates; the soil organic matter content can be greatly increased to improve the soil fertility and regulate crop growth; and finally, by improving the physicochemical property of the soil, the investment is reduced, and the yield and quality of crops are improved. Besides, the preparation method of the saline alkali soil conditioner is simple, environment-friendly and easy to operate.

The invention relates to the domain of cell engineering, in particular to a method for rejuvenating and detoxifying an edible fungal strain by using protoplast regeneration technology. The method comprises the following steps: selecting parents, preparing protoplast, regenerating the protoplast, and establishing an evaluation standard for rejuvenating and detoxifying the edible fungal strain. The step of preparing the protoplast comprises the step of selecting steady infiltration agent solute, steady infiltration agent concentration, degrading enzyme, enzymolysis time and enzymolysis temperature; and the step of regenerating the protoplast comprises the step of selecting a pre-culture medium and a culture method. The method has the advantages that the effects of removing viable bacteria in the edible fungal and inhibiting factor accumulation are achieved by obtaining the protoplast so as to fulfill the purposes of rejuvenating and detoxifying the edible fungal and recover good properties of the edible fungal. For example, the comprehensive properties of oyster mushroom 89 and pleurotus ferulae 10 are remarkably superior to those of a contrast variety in the aspects of yield, adaptability, commercial property, impure fungal resistance and the like, and the remarkable economic benefit is produced.

The invention relates to a fermentation packaging bag of animal feedstuff. The packaging bag is characterized in that because a plastic packaging bag has a breather valve, aerobic bacteria are propagated enormously during incipient fermentation of the feedstuff and gas is generated intensely with redundant gas discharged through the breather valve; when oxygen is exhausted, the breather valve stops gas discharging and the anaerobic bacteria begin to propagate; when feedstuff pH value is reduced to a fixed value, the anaerobic bacteria begin dormancy; meanwhile, traces of oxygen molecules are fed in the packaging bag continuously through a micropore on a breather valve diaphragm to meet the minimum requirements of the aerobic bacteria on survival and keep various mixed bacteria out of the packaging bag, thereby ensuring the survival of various beneficial bacteria and long-term freshness of the feedstuff. The fermentation packaging bag provides a cheapest means for obtaining large amounts of beneficial bacteria and various enzymes.

The invention discloses a facility cultivation technique for crop rotation of stropharia rugosoannulata and rice, and belongs to the technical field of edible mushroom cultivation. The facility cultivation technique includes the steps of fermentation of a culture material, construction of a mushroom shed, construction of a ridged bed, seeding, mycelium management and protection, mushroom cultivation and harvesting, and rice planting and harvesting. After the components of the culture material are subjected to composting and fermentation separately, layered laying is adopted, so that nutrient and oxygen supply in the development process of mycelia and fruiting bodies is ensured, the whole fruiting is uniform, the fruiting rate is stable, the total yield is high, the quality is good, the planting cycle is short, and the continuous cropping obstacles can be solved by crop rotation of the stropharia rugosoannulata and rice.

The invention discloses a high-density pleurotus eryngii liquid strain fermentation medium and belongs to the technical field of pleurotus eryngii cultivation. Chinese herb extracts capable of improving strain cold and heat resistant irritability, immunity and infectious microbe resistant capacity are scientifically compounded in media of all stages of liquid seeds, shaking seeds, seed tank seeds and fermentation tank liquid strains; wood chip powder with raw material adaptation and a defoamer function is scientifically compounded; the spawn age and the multiplication algebra difference are shortened, and coated chitosan with special functions is fed timely, accordingly, pleurotus eryngii liquid strains which are thick in fermentation hypha, high in activity, small in mycelium pellet diameter, large in density and uniform in distribution are cultivated, the mycelium pellet density ranges from (4.5-5.5)*105/L, the mycelium pellet diameter ranges from 0.5mm to 0.7mm, the mycelium pellet dry weight ranges from 90g/L to 100g/L, germination time is in a range of 4h to 5h, and the exopolysaccharides is 3.0g/L to 3.5g/L.

The invention provides a method for preparing cordyceps militaris, comprising the following steps of preparing strains, preparing a cultivation substrate, cultivating a strain-receiving substrate, and harvesting and drying after the strains are cultivated maturely, wherein the step of preparing the strains is performed by means of cultivating cordyceps militaris strains in the substrate, cultivating by utilization of a rotary swing bed, and cultivating at a constant temperature for a certain time till mycelium pellets are formed and uniformly float; in the step of preparing the cultivation substrate, the cultivation substrate is composed of a substrate body and a culture solution; the ingredients of the culture solution comprise beans, coconut powder, pollen, glucose, magnesium sulfate, vitamin B1, vitamin B6 and the rest of alkalescent water; the material-to-liquid ratio of the substrate body to the culture solution is 1:1.7-1.9; the moisture content of a dried product is not greater than 8%. The cordyceps militaris prepared according to the method for preparing the cordyceps militaris has the advantages that the color is golden, conidial powder is contained, the contents of cordycepin, cordyceps polysaccharide and superoxide dismutase (SOD) are high, the formula and method provided by the invention are simple and are easily obtained, the ingredients which are used for preparing the culture solution are simple and are easily obtained, and the cost is low.

The invention discloses a wheat low temperature-resistant conditioning agent and an application method thereof. The wheat low temperature-resistant conditioning agent comprises the following components in parts by weight: 18-27 parts of melatonin, 13-19 parts of potassium humate, 12-17 parts of a-naphthylacetic acid, 8-14 parts of jasmonic acid, 6-14 parts of phytohormone brassinolide, 3-7 parts of cytokinin, 2-8 parts of an antifreeze, 0.3-0.7 parts of antibacterial peptide, 1-5 parts of a dispersant, and 20-30 parts of water. The wheat low temperature-resistant conditioning agent has the beneficial effects that the conditioning agent takes water as a medium, has obvious environment benefit and social benefit, can increase the security; protects a cell membrane, stabilizes the structure of biomacromolecules such as antioxidant enzyme, enhances capability for effectively removing ROS by cells, reduces intracellular water potential and ice point, and enhances the anti-pathogen capability of the wheat; the active ingredient source is abundant, the active ingredient is regenerable, is easily degraded in natural environment, has the characteristics of low cost and convenient usage, and conditioning agent has the advantages of easy popularization and application.

The invention discloses a pleurotus eryngii liquid strain with small and many mycelium pellets and a fermentation method of the pleurotus eryngii liquid strain, and belongs to the technical field of pleurotus eryngii cultivation. Chinese herb extracts capable of improving cold resistance, heat irritability resistance, immunity and bacterium-resisting capacity of the strain are scientifically compounded in culture mediums for liquid seeds, shaking flask seeds, seeding tank seeds and fermentation tank liquid strains in all stages, and meanwhile wood powder which has the feedstock adaptability and has the function of an antifoaming agent is scientifically compounded; manners of supplemental inoculation and segmentation variable temperature fermentation are adopted, and therefore the difference between the strain cell age and the multiplication algebra is shortened; and packaged chitosan with a special function is properly fed, and the pleurotus eryngii liquid strain with the thick and solid fermentative hyphae, high activity, small mycelium pellet diameter, large density and uniform distribution is cultured. The density of the mycelium pellets ranges from 4.5*10<5>piece/L to 5.5*10<5>piece/L, the diameter of the mycelium pellets ranges from 0.5mm to 0.7mm, the dry weight of the mycelium pellets ranges from 90g/L to 100g/L, the germination time ranges from 4 hours to 5 hours, and the exopolysaccharide ranges from 3.0g/L to 3.5g/L.

The invention discloses a planting method of high-quality Chinese cabbages. The planting method mainly comprises the following steps: land treatment, seed soaking treatment, sowing, seedling stage management, field management, and harvesting and picking. Compared with the prior art for planting Chinese cabbages, the planting method disclosed by the invention has extremely significant advantages. The above technical scheme adopted to plant the Chinese cabbages is green, environment-friendly and pollution-free, and the Chinese cabbages can grow fast and vigorously, and have strong stress resistance; the planting method disclosed by the invention adopts an artificial method for planting the Chinese cabbages, and the treatment of Chinese cabbage seeds can improve the quality of the seeds, control diseases and insects, break the dormancy of the seeds, and promote neat germination of the seeds and vigorous growth of seedlings. Seedling stage treatment not only can promote the growth of plants and the growth and development of the Chinese cabbage seeds in the rosette stage so as to improve the yield of the Chinese cabbages, but also can improve the absorption and accumulation of trace elements by the Chinese cabbages and improve the nutritive value of the Chinese cabbages. The Chinese cabbages planted by the planting method disclosed by the invention have high quality, high yield and high economic benefits.

The invention discloses an easy-to-digest and growth-promoting ecological feed for lobsters. The feed is prepared from wheat flour, broad beans, sweet corn flour, chicken intestine meal, beetle meal, shell powder, oenanthe javanica, algae powder, decavitamin, chlorine chloride, betaine, lactobacillus, aspergillus niger, beef tallow, fish oil, xylanase, phytase and an additive, wherein the additive is prepared from raw materials as follows: radix astragali, herba houttuyniae, dried tangerine or orange peels, quisqualis indica, hawthorn fruits, radix isatidis, leucaena glauca, clover, glutathione, octacosanol, lysoph-osphatidylcholine, L-magnesium aspartate, zinc sulfate, linolenic acid, linoleic acid and peptidoglycan. The easy-to-digest and growth-promoting ecological feed for the lobsters is balanced in nutrition and easy to digest, can promote growth of the lobsters, enhances immunity of the lobsters and reduces the incidence rate.