High-density pleurotus eryngii liquid strain fermentation medium

A fermentation medium and liquid strain technology, applied in fertilizer mixture, fertilization device, application, etc., can solve the problems of small mycelial ball diameter and short fermentation cycle, and achieve small mycelial ball diameter, strong fermented mycelium, The effect of increasing yield

Active Publication Date: 2015-08-19
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem solved by the present invention is to overcome the defects of the existing Pleurotus eryngii liquid strains prepared by aerated fermentation, and the scientific compounding in the culture medium of each stage of the strain cultivation process can improve the strain resistance to cold, heat stress, and anti-coldness.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1 raw material preparation

[0050] 1. Preparation of Chinese herbal medicine extract:

[0051] The preparation method of the Chinese herbal medicine extract comprises the steps of: weighing 30 parts of astragalus, 30 parts of dried ginger, 30 parts of tangerine peel, 25 parts of bezoar, 25 parts of paeonol, 20 parts of licorice, and 18 parts of anemarrhena in parts by weight. 18 parts of honeysuckle, 17 parts of Houttuynia cordata, 15 parts of red peony, 15 parts of scutellaria baicalensis, 10 parts of Chuanxiong, and 8 parts of angelica; put them in an ultrasonic cleaner filled with 0.2% sodium bicarbonate solution for 12 minutes at 200W and 30KHz, Drain, crush the above-mentioned Chinese herbal medicine to a particle size of less than 2 mm, put it in a container and mix evenly and add 5 times the weight of water to obtain a mixed material, control the temperature at 80°C for 3 hours, then lower the temperature to 45°C, and adjust the pH value with lactic a...

Embodiment 2

[0059] A kind of method utilizing fermented medium to prepare high-density Pleurotus eryngii liquid strain, comprises the steps:

[0060] 1) Take the preserved Pleurotus eryngii slant strain 0.2cm 2 Inoculate 2 pieces of the strain block of the above-mentioned bacteria on the plate medium for activation, and culture at a constant temperature of 25°C for 10 days, and activate twice in this way to obtain plate seeds;

[0061] 2) Use a puncher with a diameter of 0.5cm to intercept the flat seed 0.2cm 2 3 pieces of the strain block were inserted into a 250mL Erlenmeyer flask, and the amount of liquid seed culture medium was 100mL. They were cultured at a constant temperature of 25°C for 2 days, then placed in a constant temperature shaker, and shaken at 25°C and 100r / min for 3 days. liquid seeds;

[0062] 3) The liquid seeds were inoculated in a 500 mL shake flask with an inoculum amount of 10%, and the liquid volume of the shake flask culture medium was 250 mL, and cultured at ...

Embodiment 3

[0073] A kind of method utilizing fermented medium to prepare high-density Pleurotus eryngii liquid strain, comprises the steps:

[0074] Step 1) to 4) with embodiment 2;

[0075] 5) First inoculate the seeds of the seed tank with 8% inoculum amount in a 30L fermenter, the fermenter medium capacity is 15L, and ferment at 30°C at a constant temperature of 3L / min for 12h, and then cool down at a rate of 0.3°C / min to 18°C, add 10g coated chitosan and 900mL seed tank seeds into the fermenter, ferment at constant temperature for 10h under the condition of ventilation rate 6L / min, and finally raise the temperature to 24°C at the rate of 0.2°C / min Ferment at constant temperature for 45 hours under the condition of 1 min to obtain high-density Pleurotus eryngii liquid strain;

[0076] Described plate medium composition is the same as embodiment 2;

[0077] The liquid seed medium consists of: glucose 30g / L, yeast extract juice 5g / L, peptone 2g / L, agar 2g / L, Chinese herbal medicine ex...

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Abstract

The invention discloses a high-density pleurotus eryngii liquid strain fermentation medium and belongs to the technical field of pleurotus eryngii cultivation. Chinese herb extracts capable of improving strain cold and heat resistant irritability, immunity and infectious microbe resistant capacity are scientifically compounded in media of all stages of liquid seeds, shaking seeds, seed tank seeds and fermentation tank liquid strains; wood chip powder with raw material adaptation and a defoamer function is scientifically compounded; the spawn age and the multiplication algebra difference are shortened, and coated chitosan with special functions is fed timely, accordingly, pleurotus eryngii liquid strains which are thick in fermentation hypha, high in activity, small in mycelium pellet diameter, large in density and uniform in distribution are cultivated, the mycelium pellet density ranges from (4.5-5.5)*105/L, the mycelium pellet diameter ranges from 0.5mm to 0.7mm, the mycelium pellet dry weight ranges from 90g/L to 100g/L, germination time is in a range of 4h to 5h, and the exopolysaccharides is 3.0g/L to 3.5g/L.

Description

technical field [0001] The invention relates to the technical field of pleurotus eryngii cultivation, in particular to a high-density pleurotus eryngii liquid strain fermentation medium. Background technique [0002] Pleurotus eryngii (PleurotuseryngiiQuel.), also known as Pleurotus eryngii, has the advantages of thick flesh and crisp texture, especially its dense, firm, milky white and refreshing stipe. It is called "king of oyster mushrooms" and "scallop mushrooms". , with almond fragrance and abalone taste, suitable for fresh-keeping and processing, and is deeply loved by people. It is rich in nutrients, rich in protein, carbohydrates, vitamins and minerals such as calcium, magnesium, copper, zinc, etc., which can improve human immune function. It has the effects of anti-cancer, lowering blood fat, moisturizing the stomach and beautifying the human body, and has extremely high cultivation value and commercial value. [0003] At present, the cultivation of Pleurotus eryng...

Claims

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Application Information

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IPC IPC(8): C05G3/00
Inventor 班立桐王玉黄亮杨红澎
Owner TIANJIN AGRICULTURE COLLEGE
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