402 results about "Ganoderma pseudoferreum" patented technology

The invention provides a method of effectively extracting ganoderma triterpenoids and ganoderan from mythic fungus germ entity by ultrasonic-alcohol extracting, complex enzyme-water extracting integration technology. The method is characterized by comprising the steps of: crashing the mythic fungus germ entity, using the ultrasound assisted alcohol extract (ultrasonic-alcohol extracting) to extract the ganoderma triterpenoids, then using complex enzyme assisted water extracting (complex enzyme-water extracting) to prepare ganoderan, then sponging drying to obtain ganoderma triterpenoids and ganoderan extractive. The solvents used by the method used in the invention is safe, innoxious and avoids the pollution to the environment by using chloroform, methanol and other organic solvents in the traditional method. And the invention has the features of low extraction temperature, simple device, convenient operation, low cost, fast speed, high effects and so on. Using the process to comprehensively extract triterpenoids and ganoderan active substances in glossy ganoderma can significantly increase the total active substance yield than the single extracting method.

The invention is a method of preparing glossy ganoderma triterpenic acid and amylase by glossy ganoderma, crushing the dry glossy ganoderma seeds (water content 5-8%) into particles; or cutting into slices, then putting raw material in a high-pressure extraction kettle, then injecting CO2 and polar agent in the extraction kettle by a supercritical CO2 high-pressure pump and a polar agent pump, respetively, where the extracting pressure of the high-pressure pump is 25-50 MPa, the injection quantity of the polar agent in bulk percent is 10-20%, the temperature is 35-80 deg.C, and the extracting time 2-4 hours, obtaining the extract of glossy ganoderma triterpenic acid; adding water by 8-16 times in the residues, heating to 80-100 deg.C, extracting for 1-2 hours, obtaining the extract of glossy ganoderma amylase. In the products prepared by this invention, the content of glossy ganoderma triterpenic acid is up to 60.2% (by HPLC detection); that of glossy ganoderma amylase 36% (by UV spectral detection).

The invention provides a method for cultivating lucid ganoderma, relating to a method for cultivating edible fungi, in particle to a method for cultivating the lucid ganoderma with new cultivating material. The cultivating material in the method contains eucalyptus, wherein the cultivating material is preferably contains eucalyptus bark or eucalyptus branch or mixture of the eucalyptus bark and the eucalyptus branch. The method selects the eucalyptus as the cultivating material, wherein the eucalyptus is not usually regarded as to be suitable for cultivating the lucid ganoderma, to obtain lucid ganoderma seed entities with faster growth speed, wherein the seed entities almost have the same effective constituent of the lucid ganoderma. Furthermore, the eucalyptus has fast growth, short circulation period and high yield, therefore, the price is lower than the other broad-leaved trees. Because the other constituents such as cotton seed hulls, bagasse, and the like, are not needed to be added, comparatively speaking, the method has lower price.

The invention relates to the edible mushroom culturing technology, in particular to a method for culturing lucid ganoderma by using oral liquid dregs. The method comprises the following steps of: (1) crushing of oral liquid dregs; (2) preparation of a culturing material; (3) bag packaging and sterilization of the culturing material; (4) inoculation of lucid ganoderma strains; (5) culturing of mycelia; and (6) management of lucid ganoderma output. Compared with the conventional lucid ganoderma culturing method, the method disclosed by the invention has the following advantages: 1) environmental friendliness: according to the method disclosed by the invention, lucid ganoderma culturing is realized by using the oral liquid dregs, so that the consumption of natural resources, such as woods and the like, is avoided, and the dregs are sufficiently used without polluting environment no more; 2) economy: according to the method disclosed by the invention, waste is turned into wealth, so that the cost for treating the oral liquid dregs is saved, and an economical value is generated by using the oral liquid dregs; and 3) good culturing effect: compared with the conventional lucid ganoderma culturing method, the benefit ratio of the lucid ganoderma culturing method disclosed by the invention is not smaller than that of the conventional culturing method.

The invention discloses a method for cultivating mulberry twig glossy ganoderma. The method comprises the following steps of: (A) bagging; (B) sterilizing and inoculating; (C) culturing; (D) inducing primordia; (E) managing mushroom culturing; (F) grafting; (G) performing aeration drying; and (H) spraying paint and boxing. The mulberry twig glossy ganoderma cultivated with the method provided by the invention has the characteristics of attractive appearance, good ornamental effect and high reserve value, and is beneficial to the expansion of virtuous circle of the silkworm industry; and the planting yield of ganoderma is greatly increased.

An immunomodulatory protein is cloned from Ganoderma microsporum. Its molecular weight is 15863.79 Da. Its genome sequence and translated protein sequence are different from those protected by the known patent of glycoprotein LZ-8, which is isolated from the mycelium of G. lucidum and has immunomodulator effect, and its immunomodulator efficiency is better than that of LZ-8.

The method for preparing probiotics is characterized by that it utilizes the metabolic product characteristics of various strains to select the strain for mixed fermentation, in the culture of mixed strain of probiotics of bifidobacterium and lactic acid bacteria, etc. the fungous fermenting liquor of ganoderma, etc. is added to make co-fermentation, so that it not only can raise number of ermentaing living bacteria, prolong the suvival time of living bacteria, but also can retain all the nutrients of fermenting liquor, reduce product cost and retain health-care effect of said product.

The invention discloses a lucid ganoderma substitute cultivation medium with momordica grosvenori. The lucid ganoderma substitute cultivation medium comprises, by weight, 35-70 parts of sawdust, 25-60 parts of auxiliary material, 0.5-1.5 parts of gypsum, 0.1-1 part of sugar and 1-5 parts of momordica grosvenori, and the proportion of the materials to water is 1: 1.3-1.5. By means of the lucid ganoderma substitute cultivation medium, the growth of infectious microbe can be restrained very well in the cultivating process of lucid ganoderma, and the lucid ganoderma substitute cultivation medium has an obvious effect.

The invention relates to a method for preparing a lucid ganoderma sporophore fermented product, the fermented product and application thereof. The method provided by the invention comprises the following steps: mixing lucid ganoderma sporophores and water and pulping to obtain lucid ganoderma pulp; inoculating a yeast in the lucid ganoderma pulp and fermenting at 20 to 30 DEG C for 48 to 72h in adark place, so as to obtain a lucid ganoderma fermented solution; inoculating lactic acid bacteria into the lucid ganoderma fermented solution and fermenting at 40 to 43 DEG C for 48 to 72h; after fermentation is finished, obtaining the lucid ganoderma sporophore fermented product. The method provided by the invention can be used for preparing the lucid ganoderma sporophore fermented product withhigh nutrient value, strong anti-oxidization performance and good whitening effect.

The invention discloses a breeding method for natural ganoderma lucidum, and belongs to the technical field of edible fungus genetic breeding. The breeding method comprises the following steps of 1 natural ganoderma lucidum collecting, 2 test tube slant culture medium preparing, 3 tissue separating, 4 strain inoculating and cultivating, and 5 inoculating and cultivating. The natural ganoderma lucidum separated by the breeding method is large in mother strain shape, high in yield and high in sundry fungus resisting capacity, the cultivation and expanding problem of the natural ganoderma lucidum can be solved, and the finished product yield is high.

The invention discloses a potted lucid ganoderma cultivating method. The potted lucid ganoderma cultivating method includes steps of inoculating a mother strain of lucid ganoderma into culture medium in a mother strain test tube, culturing the test tube at the temperature of 22-28 DEG C for five to eight days, inoculating hypha of the mother strain of the lucid ganoderma in 1-2cm2 into a stock culture medium of the test tube, culturing at the temperature of 23-32 DEG C and the humidity of 60-70% for 32-38 days, obtaining stock hypha of the lucid ganoderma to be inoculated into a matrix of a cultivating bag, keeping out of the sun and culturing at the temperature of 25-28 DEG C and in the humidity of 85-95%, after obtaining strain sticks of the lucid ganoderma, transplanting the strain sticks into a container with nutrient solution and / or water and cultivating. The provided potted lucid ganoderma cultivating method is simple, easy to implement and widely applicable to medium- and small-size potted cultivation in greenhouses, indoor environment or households. The invention further discloses culture medium used for cultivating the lucid ganoderma. By the aid of the complex synchronous fermenting technology, nutritional ingredients of the lucid ganoderma can be maximally developed and bacteriostasis function is provided.

The invention discloses a fermentation method for promoting mythic fungus acid or mythic fungus polysaccharide biology to synthesize. The invention investigates the condition which is needed in the growth phase of mythic fungus cell and product synthesis phase, which respectively investigates the influence of control strategies of pH two phases and oxygen dissolution two phases to mythic fungus acid or mythic fungus polysaccharide biology according to unconsistency of environmental conditions which are needed in the phases of mythic fungus cell growth and production synthesis, and the invention respectively investigates the influence to mythic fungus acid or mythic fungus polysaccharide biology synthesis after control strategies of pH two phases and oxygen dissolution two phases or the fed strategy are mutually coordinated together on the basis of combining with an intermediate fed strategy. The test result shows that mythic fungus cells are obviously prompted to grow no matter respectively adopting a pH value two phases control strategy, an oxygen dissolution concentration two phases control strategy, or coordinating the pH value two phases control strategy and the oxygen dissolution concentration two phases control strategy with the fed strategy together, and the content or yield of mythic fungus acid or mythic fungus polysaccharide are greatly improved.

The invention relates to an artificial cultivation method of lucid ganoderma, in particular to an artificial cultivation method capable of realizing highland cultivation of wild ganoderma tsugae murr. The artificial cultivation method comprises the following steps: (1), a stock culture is prepared; (2), a pre-culture spawn is prepared; (3), a production spawn is prepared; (4), inoculating and cultivation are conducted; and (5), ganoderma tsugae murr growing management is conducted till a white edge of a fungi pileus is turned to yellow completely, and then the ganoderma tsugae murr is picked. According to the artificial cultivation method, the total biotransformation rate can reach 55-85%; the taste of the obtained ganoderma tsugae murr is relatively bitter; the ganoderma tsugae murr has the taste of ganoderma lucidum, and has effects of improving the immunity, preventing a cancer and the like; and requirements of the ganoderma tsugae murr in a wide range can be met through the artificial cultivation.

The invention relates to an eatable fungus cultivation method, in particular to a ganoderma lucidum culture medium, comprising the components of saw dust, wheat bran, corn powders, gypsum powders and light calcium carbonate. The ganoderma lucidum culture medium is characterized by further comprising ganoderma licidum dregs which can replace the saw dust completely. The ganoderma lucidum culture medicum has the advantage of fundamentally solving the inconsequent problems of strains in traditional ganoderma lucidum production, thereby being beneficial to the forest protection, environment protection, energy saving, emission reduction and recycling so as to ensure the sustainable development of ganoderma lucidum production, and moreover, the content of the medicinal effective components of ganoderma lucidum cultured by using ganoderma lucidum dregs is high.

The invention discloses an ecological cultivation method for ganoderma, belonging to the technical field of ganoderma cultivation. The main point of the technology is that the method comprises the following steps: (1) selecting proper maple, encapsulating and sterilizing the maple after the maple is cut into segments and cooling the maple for standby after the sterilization treatment; (2) inoculating the prepared maple segments in ganoderma strains and cultivating the ganoderma strains for 20-40 days in a dark environment under the condition of the constant temperature of 25-30 DEG C; (3) selecting a forest land with soil and air free of pesticide residue and excessive heavy metal after detection; (4) planting fruit trees on the selected forest land; and (5) transplanting the maple segments for cultivating the ganoderma strains in the soil below the fruit trees in the forest land when the outdoor temperature is above 26 DEG C, and opening bags after covering the maple segments for 5-10 days through agricultural films after the planting, wherein the ganoderma strains can be ripe after 18-25 days. The invention aims at providing the ecological cultivation method for the ganoderma, wherein the planting method is simple and convenient, the pilei of ganoderma fruiting bodies are thick, wide and large, and the nutrition value is high; therefore, the ecological cultivation method is used for the cultivation of the ganoderma.

The present invention discloses an industrial cultivation method of ganoderma. Said method includes the following steps: preparing culture medium, inoculation, hypha culture, cultivation, growing ganoderma and picking.

A lucid ganoderma pocket planting fungus bag is mainly prepared from the following raw materials by weight percentage: 75-80% of mulberry wood, 10-16% of cottonseed hull, 3-5% of wheat bran and 0.8-1.2% of gypsum powder which are bagged. The water content of the lucid ganoderma pocket planting fungus bag is 55-65%, and a pH (potential of hydrogen) value is 6.5-7.5. According to the fungus bag, high-quality and high-yield lucid ganoderma is produced by making full use of a mulberry stem resource and a climatic environment of Liucheng of Guangxi, and the lucid ganoderma planted by the fungus bag is good in color and taste and bright in color.

A Lucid Ganoderma cultivation bag material comprises, by weight percent, 25-35% of beanstalk crumbs, 20-30% of soybean dregs, 33-53% of sawdust, 2% of land plaster, and soybean whey which is 1-1.2 times of the cultivation bag material in weight. A method for cultivating Lucid Ganoderma by the Lucid Ganoderma cultivation bag material includes firstly, performing moist heat sterilization to the Lucid Ganoderma cultivation bag material at high temperature and high pressure, cooling, and inoculating a Lucid Ganoderma strain; secondly, cultivating with mycelia for 35-50 days to sprout; and thirdly, continuing to cultivate for 30 days, and sprouting and controlling to obtain ripe Lucid Ganoderma fruiting bodies. The soybean dregs contain sufficient phosphorus and vitamin, and the pH value, variation with time, of the soybean dregs fits with required environment for growth of Lucid Ganoderma. By the Lucid Ganoderma cultivation bag material, high-quality and high-yield Lucid Ganoderma fruiting bodies can grow, waste utilization of the beanstalk, the soybean dregs and the soybean whey is achieved, and primary chemical and medicinal compositions of the cultivated Lucid Ganoderma and content of Lucid Ganoderma triterpenes are higher than those of marketable Lucid Ganoderma.

The invention discloses a supercritical fluid extract technology of ganoderma volatile oil, ganodenic acid and ganoderma polysaccharide, which comprises the following steps: (1) drying and crushing ganoderma mycelium or ganoderma sporocarp; (2) adding ganoderma microbe powder into the extraction cauldron of a supercritical CO2 fluid extracting machine, setting the extracting pressure to be 15-25 MPa and the temperature to be 38-42 DEG C, leading 95-105L / min CO2 fluid, extracting for 1.5-2.5h, and obtaining the ganoderma volatile oil form a separation kettle; and (3) adjusting the extracting pressure to be 28-32 MPa and the extracting temperature to be 44-47 DEG C, taking distilled water as the entrainer, extracting for 1.5-2.5h, obtaining the water solution containing the ganodenic acid and ganoderma polysaccharide, adding 3.5-4.5 times of volume of 95% (V / V) ethanol in the solution to generate flocculent precipitate, placing for 3-6h, centrifugalizing, separating, freezing and dryingthe precipitate, thus obtaining the ganoderma polysaccharide product; decompression-concentrating the centrifugalized liquid supernatant to obtain the extract containing the ganodenic acid. The supercritical fluid extract technology has simple operation, small organic solvent usage, high extraction efficiency, high recovery rate of active ingredients, good product quality and low production cost.

The invention discloses a health wine and a health tea containing saussurea involucrate, and preparation methods thereof; the health wine and the health tea mainly comprise the following raw materials in parts by weight: 5 to 25 parts of saussurea involucrate, 5 to 25 parts of medlar, 5 to 15 parts of longan meat, 5 to 15 parts of angelica, 20 to 40 parts of chrysanthemum, 5 to 15 parts of herba cistanches, 10 to 30 parts of safflower, 5 to 25 parts of glossy ganoderma, 5 to 15 parts of rhodiola, 4 to 9 parts of Chinese caterpillar fungus, 4 to 9 parts of longspur epimedium and 5 to 25 parts of pomegranate; and the ethanol extract of the raw materials are mixed with the wine and drunk or the raw materials are crushed, mixed with tea, made and drunk. The invention has good health care efficiency, has the functions of supplementing deficiency of vital energy and strengthening the body resistance, building a strong body, warming kidney and activating yang, tonifying qi and nourishing the blood, maintaining beauty and keeping young, strengthening the immune function of the body and prolonging life after being taken for a long time.

The invention discloses a method for increasing the content of intracellular ganoderma lucidum triterpenes during liquid fermentation of ganoderma lucidum mycelia. The method is characterized by comprising the steps of performing enlarged cultivation, adding a wood degradation product into a culture solution according to a mass proportion of 0.01 to 5 percent, and performing culture for 1 to 6 days. According to the method for increasing the content of the intracellular ganoderma lucidum triterpenes during liquid fermentation of the ganoderma lucidum mycelia, microcrystalline cellulose is added to induce ganoderma lucidum to produce more intracellular ganoderma lucidum triterpenes during liquid fermentation of the ganoderma lucidum mycelia; the adopted inducer microcrystalline cellulose is safe and low in cost, and the content of the intracellular ganoderma lucidum triterpenes can be remarkably increased.

The invention relates to the field of novel cultivation techniques of edible fungi, in particular to a cultivation method of large Ganoderma lucidum. The method includes the steps of using broad-leaved wood 6-30cm in diameter as raw material in October to November each year, sawing the wood into short segments, chopping the short segments into irregular wood blocks (sheets), bagging, inoculating, and cultivating fungus cylinders; performing earthing cultivation on the fungus cylinders in a light environment which can be 70% shady and 30% shinny in March of the next year; setting 15 fungus cylinders in a group during cultivation, and cultivating one mushroom of large Ganoderma lucidum with cap diameter up to 80-120cm by all mycelia and nutrients in the 15 fungus cylinders; after the first mushroom of large Ganoderma lucidum is picked, cultivating a second mushroom of large Ganoderma lucidum with the cap diameter up to 40-60cm in the same management way as the first mushroom of large Ganoderma lucidum. Through the application of the method, fruiting bodies of the cultivated large Ganoderma lucidum are large and magnificent with high quality and benefits produced are extraordinarily significant.

The invention discloses a method for cultivating wood log cultivated ganoderma lucidum spore powder with rich selenium, pertaining to the field of cultivating edible (medicinal) fungi. The method mainly includes: mixing the inorganic selenium, ganoderma lucidum fungi and ganoderma lucidum culture medium according to certain proportion, through biotransformation of ganoderma lucidum fungi, improving the content of organic selenium in spore powder. The steps for cultivating wood log cultivated ganoderma lucidum spore powder with rich selenium are as follows: adding sodium selenite containing inorganic selenium for cultivating ganoderma lucidum fungi with rich selenium, adding sodium selenite for preparing ganoderma lucidum culture medium with rich selenium, inoculating ganoderma lucidum fungi, inorganic selenium entering the interior of the wood logs through ganoderma lucidum mycelium to realize biotransformation, cultivating ganoderma lucidum with rich selenium and ganoderma lucidum spore powder with rich selenium. The content of the organic selenium in the spore powder cultivated by this method can reach to 51mg / kg-130mg / kg. After people take the spore powder, the level of blood selenium and immunity can be improved greatly, and the spore powder has good effect of illness treatment and health care.

The invention discloses a method for cultivating lucid ganoderma or auricularia polytricha. A culture medium comprises 10 to 90 weight percent of debarked magnolia officinalis branch wood pellets, the total C / N ratio of the culture medium is (50-80):1, and the pH value is 5 to 8. The method comprises the following steps of: uniformly mixing the magnolia officinalis branch wood pellets which are fully soaked with water and other components of the culture medium, sterilizing and inoculating; and culturing under environmental conditions adapting to the growth habit of the lucid ganoderma or auricularia polytricha strains so as to obtain the finished product of the lucid ganoderma or auricularia polytricha. The method solves the problem of selection of culture media during conventional cultivation of the lucid ganoderma or auricularia polytricha, also solves the problem of recycling massive debarked magnolia officinalis branches which are used as medicines, and changes waste materials into valuable things; meanwhile, the conventional cultivating and operating method is not needed to be changed, the yield of the lucid ganoderma or auricularia polytricha can be improved, and the method has high social significance and commercial value.

The invention relates to a health care food containing fermentation cordyceps and lucid ganoderma, capable of enhancing organism immunity. Paecilomyces hepiali Chen and ganoderma lucidum karst are respectively subjected to liquid deep fermentation to obtain mycelium and fermentation liquor, then spray rotary fluidization is carried out for dry pelleting, and the diameter is controlled at 500-800mu m. The ratio of cordyceps fermentation powder and lucid ganoderma fermentation powder according to parts by weight is 1:1-4:1. The content of cordycepin in the product is greater than or equal to 1.5mg / g, the content of polysaccharide from cordyceps is greater than or equal to 2.0mg / g, the content of adenoside is greater than or equal to 4.0mg / g, and the content of polysaccharide from lucid ganoderma is greater than or equal to 1.5mg / g. The invention uses spray rotary fluidization for dry pelleting, can fully retain fermentation liquor and mycelium in the product without concentration and extraction, has simple technology, avoids wasting nutrient, generates no waste liquor and waster residue, and has evenly distributed particles of the finished product and excellent intensity and solvability. The health care food of the invention has obvious effects on deficiency of the kidney, deficiency of vital energy, deficiency of the lung and cough, and has the functions of strengthening the bones and muscles, dispelling fatigue, calming the nerves, resisting tumour and the like.

The invention discloses a simulated wild ganoderma atrum tree section culturing technology. The simulated wild ganoderma atrum tree section culturing technology includes firstly preparing a culturing material bag; selecting a hard broad-leaved tree, sawing the hard broad-leaved tree into short sections, packaging the short sections and disposing the same into a steam pot to steam; secondly, sterilizing: sterilizing a seeding chamber with blended agentia ; thirdly, seeding: cooling the culturing material bag and grafting hyphae thereon; fourthly, culturing the hyphae; fifthly, digging a rest-balk: digging the rest-balk on loose soil, and digging a ganoderma atrum bed in the rest-balk; sixthly, covering earth; seventhly, managing; and eighthly, harvesting. The simulated wild ganoderma atrum tree section culturing technology is simple and easy and low in economical cost, simulated wild ganoderma atrum cultured is high in effective components and fine in economic benefits and has wide development prospect.

The invention discloses a ganoderma cell culture method for promoting the biosynthesis of Ganodenic acid and ganoderma lucidum polysaccharide. The method comprises the steps as follows: inoculating the strains of ganoderma into a slant to carry out culturing; then carrying out first grade liquid seed culture, second grade liquid seed culture and liquid submerged fermentation in sequence; wherein, visual light is used to carry out light culture in the liquid submerged fermentation. By determining, the method can effectively promote the growth of the ganoderma cells. Compared with the existing cell culture method, the content or yield of the Ganodenic acid and the ganoderma lucidum polysaccharide in the cell culture product prepared by the method is improved to different degrees.

The invention relates to a ganoderma lucidum culture medium which is characterized by comprising the following components in percentage by mass: 30-40% of eucalyptus, 20-30% of mulberry, 10-20% of cottonseed hull, 10-20% of ganoderma lucidum slag, 5-10% of bran, 1-5% of corn flour and 1-3% of calcium carbonate. The culture method comprises the following steps: (1) material preparation; (2) proportioning: mixing the eucalyptus wood dust, mulberry wood dust, cottonseed hull, ganoderma lucidum slag, bran, corn flour and calcium carbonate, adding water which is 1.3-1.7 times by mass of the culture medium, and uniformly mixing; (3) fermentation: piling the mixed culture medium, and fermenting at normal temperature for 8-12 hours to obtain the culture medium; (4) bagging; (5) culturing for 30-35 days to grow the ganoderma lucidum; and (6) after 55-60 days, carrying out mushroom emergence management to obtain the mature ganoderma lucidum sporophore. By using the low-cost mulberry and eucalyptus to culture the ganoderma lucidum, the cultured ganoderma lucidum has high nutrient content, and the production cost is lowered.

The invention disclose ganoderma lucidum kudzuvine root juice which takes ganoderma lucidum liquid and pueraria thomsonii benth as raw materials and comprises the following components: 30-200mg / L of pueraria isoflavone, 13.8-30g / L of ganoderma lucidum polysaccharide and not more than 50g / L of kudzuvine root powder; and meanwhile honey and glycyrrhizic acid can be also blended into the materials. The ganoderma lucidum kudzuvine root juice reserves puerarin, cellulose, protein and the like in the pueraria thomsonii benth maximally, lowers starch content as much as possible and transforms starch, protein and the like into ganoderma lucidium mycelium through fermentation, thus meeting the need of components for health of modern people; and meanwhile the honey and liquorice are added during the preparation process, thus being healthcare beverage which meets the healthcare need of modern sub-healthy population or alcoholic liver population.

A process for extracting the active components from ganoderma spore by supercritical CO2 includes mixing the ganoderma spora with its sporophores, adding edible alcohol, introducing supercritical CO2, and extracting. Its advantage is high extracting rate.