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Glossy ganoderma cell culture method for accelerating biosynthesis of ganoderic acid and ganoderma iucidum polysaccharide

A cell culture, Ganoderma lucidum polysaccharide technology, applied in the field of microbial fermentation, can solve the problems of unseen light cell growth, the physiological and biochemical effects of product synthesis cells, etc.

Inactive Publication Date: 2009-07-29
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the liquid submerged fermentation process of medicinal fungi, there are no relevant reports on the effects of light on cell growth, product synthesis, and even cell physiology and biochemistry.

Method used

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  • Glossy ganoderma cell culture method for accelerating biosynthesis of ganoderic acid and ganoderma iucidum polysaccharide
  • Glossy ganoderma cell culture method for accelerating biosynthesis of ganoderic acid and ganoderma iucidum polysaccharide
  • Glossy ganoderma cell culture method for accelerating biosynthesis of ganoderic acid and ganoderma iucidum polysaccharide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] According to the following culture medium and culture conditions, the culture of ganoderma lucidum slant strains, first-level liquid seed culture, second-level liquid seed culture and liquid submerged fermentation were carried out:

[0032] Described slant culture medium is PDA agar medium (magnesium sulfate heptahydrate of 1.5 grams / liter, potassium dihydrogen phosphate of 3.0 grams / liter, glucose of 20 grams / liter, vitamin B2 of 0.05 grams / liter 1 and 200 g of potatoes / liter of extract). The cultivation conditions of the ganoderma lucidum slant strains are: cultivation temperature 28° C., dark cultivation, and cultivation time 7 days.

[0033] The first-level liquid seed culture medium is: 35 grams / liter of glucose, 5 grams / liter of peptone, 2.5 grams / liter of yeast powder, 0.5 grams / liter of magnesium sulfate heptahydrate, 1 gram / liter of potassium dihydrogen phosphate, vitamin B 1 0.05 g / l. Primary liquid seed culture conditions: temperature 30°C, rotary shaker, r...

Embodiment 2

[0044] This test has carried out 4 groups of parallel Ganoderma lucidum cell culture experiments, and the culture medium and culture condition of each group are all the same as embodiment 1, the difference is: in the liquid submerged fermentation, each group adopts light intensity respectively to be 100 ± 20 lux, 300 lux. ± 20 lux, 500 ± 50 lux, 1000 ± 50 lux white light for light culture; the cells were weighed after drying at 60 ° C, and the dried cells were determined according to Tang and Zhong (Enzyme Microb. Technol. 2002, 31: 20-28 ) method, the contents of Ganoderma lucidum polysaccharide and Ganoderma acid were determined by concentrated sulfuric acid-phenol method and ultraviolet spectrophotometry respectively. The test results are shown in Table 3.

[0045] Table 3 Effects of white light with different light intensities on content and yield of ganoderma acid and ganoderma polysaccharide

[0046]

[0047] a All values ​​are the mean of three values ​​plus standar...

Embodiment 3

[0051] This test is carried out on the basis of embodiment 1 and embodiment 2. Be divided into test group and control group, control group is dark cultivation in liquid submerged fermentation process, and other conditions are the same as test group (the culture condition of culture medium and each stage is the same as embodiment 1); Test group is divided into 3 parallel groups, the first The first stage adopts dark cultivation, and the second stage adopts white light with a light intensity of 500 lux for light cultivation. The time points from the first stage to the second stage are respectively the 2nd day, the 4th day and the 6th day when the submerged fermentation starts sky. The cells were weighed after drying at 60°C, and the dried cells were extracted and treated according to the method of Tang and Zhong (Enzyme Microb. Technol. 2002, 31: 20-28), and then the concentrated sulfuric acid-phenol method and UV spectrophotometry were used respectively. The contents of Ganode...

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Abstract

The invention discloses a ganoderma cell culture method for promoting the biosynthesis of Ganodenic acid and ganoderma lucidum polysaccharide. The method comprises the steps as follows: inoculating the strains of ganoderma into a slant to carry out culturing; then carrying out first grade liquid seed culture, second grade liquid seed culture and liquid submerged fermentation in sequence; wherein, visual light is used to carry out light culture in the liquid submerged fermentation. By determining, the method can effectively promote the growth of the ganoderma cells. Compared with the existing cell culture method, the content or yield of the Ganodenic acid and the ganoderma lucidum polysaccharide in the cell culture product prepared by the method is improved to different degrees.

Description

technical field [0001] The invention relates to a microbial cell culture method, in particular to a ganoderma cell culture method for promoting ganoderma cell growth and biosynthesis of ganoderma polysaccharides and ganoderma acid by using visible light irradiation in the ganoderma liquid submerged fermentation process, and belongs to the field of microbial fermentation. Background technique [0002] Ganoderma lucidum (Leyss ex Fr.) Krast belongs to Basidiomycetes, Polyporaceae, Ganoderma higher fungi. According to traditional Chinese medicine, Ganoderma lucidum is sweet in taste, warm in nature, and non-toxic. It can replenish the qi of the heart, liver, spleen, lung, and kidney, and has the effects of nourishing and strengthening the body, strengthening the body and strengthening the body. Modern medical research has found that the active ingredients contained in Ganoderma lucidum are mainly Ganoderma lucidum polysaccharides and Ganoderma acid. Ganoderma acid and Ganoderm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N13/00C12P19/04C12P7/40C12R1/645
Inventor 汤亚杰张伟李冬生
Owner HUBEI UNIV OF TECH
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