262results about How to "Promote biosynthesis" patented technology

A novel relationship between pancreatico-biliary secretion and autistic syndrome is disclosed. This relationship enables a novel therapy for the treatment of the symptoms of autistic syndromes, comprising the administration of a therapeutically effective, preferably intravenous, dose of secretin to an individual with autistic syndrome. The relationship further enables a differential diagnosis for autistic syndrome, comprising an analysis of an individual's blood and / or intestinal tissue for the presence of secretin and comparison of the level of secretin to known norms.

The invention relates to the technical field of fermentation engineering, and discloses a submerged fermentation technology of producing immunological enhancement active materials of inonotus obliquus. The submerged fermentation technology of producing the immunological enhancement active materials of the inonotus obliquus comprises the main steps of culture activation, culture liquid cultivation, submerged fermentation and preparation of the immunological enhancement active materials of the inonotus obliquus and the like. The optimized submerged fermentation technology of producing the immunological enhancement active materials of the inonotus obliquus is high in content of the obtained immunological enhancement active materials, and good in activity of the obtained immunological enhancement active materials, and the obtained immunological enhancement active materials are beneficial for industrial application.

The invention provides an apple facial mask based on a traditional Chinese medicine formula.The apple mask comprises a silk matrix and a water-soluble substance.The water-soluble substance comprises apple extract, traditional Chinese medicine extract, seaweed extract, sodium hyaluronate, beta-glucan and assistant.The traditional Chinese medicine extract comprises tremella extract, centella asiatica extract, polygonum cuspidatum root extract, scutellaria root extract, tea extract, glycyrrhiza glabra root extract, chamomilla recutita flower extract, rosmarinus officinalis leaf extract and purslane herb extract.The seaweed extract contains chondrus crispus and bio-saccharide gum-1.The assistant comprises butanediol, propylene glycol, diglycerol, polyethylene glycol-400, Carbomer, hydroxyethyl cellulose, urea, allantoin, glycine betaine, nicotinamide, sodium glutamate, triethanolamine, 1,2-hexanediol, decoyl hydroximic acid, 1,3-propylene glycol, hexanediol and deionized water.The apple mask has the effects that stability is good, dispersibility is high, nutrients are proper in quantity and balanced, antibiosis and anticorrosion effects are achieved, and moisturizing, skin whitening and stain lightening are achieved.

The invention discloses a pyrus transcription factor PyERF3 as well as a recombinant expression vector and application thereof. A transcription factor PyERF3 gene which is separated from 'Starkrimson'pyrus and has an effect of promoting pyrus peel anthocyanin biosynthesis has a nucleotide sequence shown as SEQ ID No.1, and a coded amino acid sequence is shown as SEQ ID No.2 in a sequence table. The transcription factor is subjected to instantaneous conversion in tobacco, strawberry and pear fruits by virtue of an agrobacterium mediated genetic transformation method. Biological function verification proves that the PyERF3 gene cloned in the invention and other transcription factors PyMYB114 and PybHLH3 form transcriptional control complex so as to promote the biosynthesis of the pyrus peelanthocyanin. Discovery of the gene PyERF3 provides novel gene resources for promoting molecular breeding of pyrus peel anthocyanin synthesis and provides novel genetic resources for implementing green agriculture. Development and utilization of the genetic resources contribute to reduction of the agricultural cost and realization of environment friendliness.

The invention discloses an 8-amino acid / 11-vitamin containing micro granule capsule and a preparation method thereof, wherein the micro granule capsule contains 7 ball micro granules consisting of the following raw materials in part by weight: 2.66-9.9 parts of thiamine mononitrate micro granule, 16.35-20.5 parts of vitamin micro granule a, 35.0-44.1 parts of amino acid micro granule a, 11.4-14.4 parts of vitamin micro granule b, 6.65-10.5 parts of amino acid micro granule b, 12.73-16.0 parts of vitamin C micro granule and 5.7-9.36 parts of vitamin AD micro bead. The method has the advantages of scientific and reasonable proportion, good stability of products, no toxicity and side effect and excellent biological effect, and can be used for the preparation of medicines treating chronic liver and kidney diseases and of food alleviating and eliminating physical fatigue caused by sports training.

The invention relates to a pet health food and a preparation method thereof. The food materials are weighed according to weight percentage, and are 60 to 80 percent of glutin, 5 to 20 percent of fish collagen peptide powder, 5 to 20 percent of fish oil and 5 to 10 percent of compound vitamin, and the compound vitamin is animal-needed vitamin which includes vitamin A, vitamin B1, vitamin D, vitamin E, vitamin C and niacin; the materials are ground, mixed, then sent into a twin-screw extrusion-expansion machine, expanded and press-formed by a die, and after drying for shaping, picking, packaging and sterilization, the finished product is obtained. The pet health food has low fat content and high high-quality protein content, and is rich in minerals and vitamins, nutrition is balanced, and after being eaten, the pet health food can ensure that pets can become bright and active and that the fur can become brighter and more smooth, and also can increase appetite and promote the growth of muscles and bones. The shape of the pet health food is full, the internal texture is hard and dense, and thereby the pet health food can satisfy the chewing nature of pets and pets can grind and clean teeth.

The invention relates to the cultivating technique for mushroom, especially to the cultivating method for white colored mushroom, comprising preparing bacterial stick, inoculating, culturing and getting mushroom. Adding germanium and selenium element in the bacterial stick preparation, and employing special managing method for sterilizing, inoculating, culturing and getting mushroom. The prepared white colored mushroom is characterized by the fast food eating, strong bacterial filament, high productivity of above 95%, early bulb bearing, fast mushroom preparation, tender mushroom, large quantity containing of organic germanium and organic selenium which is easy-to-adsorb by human, and distinctive economical benefit.

The invention discloses a ganoderma cell culture method for promoting the biosynthesis of Ganodenic acid and ganoderma lucidum polysaccharide. The method comprises the steps as follows: inoculating the strains of ganoderma into a slant to carry out culturing; then carrying out first grade liquid seed culture, second grade liquid seed culture and liquid submerged fermentation in sequence; wherein, visual light is used to carry out light culture in the liquid submerged fermentation. By determining, the method can effectively promote the growth of the ganoderma cells. Compared with the existing cell culture method, the content or yield of the Ganodenic acid and the ganoderma lucidum polysaccharide in the cell culture product prepared by the method is improved to different degrees.

The invention discloses a coding gene sequence of an AP2 / ERF transcription factor SmAP2 / ERF82 for regulating tanshinone synthesis. The SmAP2 / ERF82 gene provided by the invention has a nucleotide sequence as shown in the SEQ ID No.1. The gene encoded protein has an amino acid sequence as shown in the SEQ ID No.2. A SmAP2 / ERF82-RNAi vector and a SmAP2 / ERF82-overexpression vector are constructed in the invention. Through a genetic transformation method for transformation of red sage root, transgenic hairy roots are obtained. In comparison with a control line, contents of dihydrotanshinone I, cryptotanshinone, tanshinone I and tanshinone II A in the SmAP2 / ERF82-RNAi line are remarkably reduced, and contents of dihydrotanshinone I and cryptotanshinone in the SmAP2 / ERF82-overexpression line areremarkably raised. The SmAP2 / ERF82 provided by the invention has the function of positive regulation of tanshinone compound biosynthesis. The compounds have remarkable efficacy of treating cardiovascular diseases. The invention brings forth new ideas for the research on molecular mechanism for tanshinone synthesis regulation and lays a foundation for the biological research on synthesis of tanshinone.

This invention relates to a selenium-rich additive for Lentinus edodes. The additive is rich in Se, Ge and K, and is prepared by mixing sodium selenite, germanium dioxide, potassium dihydrogen phosphate and water. The additive can promote Lentinus edodes growth, improve stress resistance, and increase Lentinus edodes yield and selenium content. The additive is good for health, and can prevent cancer, endemic goiter, diabetes, etc. Besides, the additive also has such advantages as no toxicity, no pollution, obvious yield increase effect, and convenient application.

The invention discloses a method for increasing the content of mogroside IV. The method comprises the following steps: I, preparing a solid culture medium containing jasmonic acid methyl ester, wherein the concentration of the jasmonic acid methyl ester is 50-400 [mu] mol / L before the solid culture medium is cured; and II, inoculating momordica grosvenoritissue culture seedlings into the solid culture medium, culturing for 28-35 days, and transplanting the momordica grosvenori culture seedlings into fields. The method has the characteristics of being rapid in effect taking, low in cost, simple to operate, convenient to implement, free of toxin or reside in products, and the like, and by adopting the method, the yield of the mogroside IV can be effectively increased, and large-scale production of the mogroside IV can be achieved.

The invention belongs to the field of light industry, in particular to a quick-drying type plant scar removal, black removal and restoration membrane which comprises the following raw materials in percentage by weight: 0.5-1% of chitosan quaternary ammonium salt, 1-2% of polyvinylpyrrolidone, 0.5-1% of centella asiatica extractive, 0.3-0.7% of astragaloside, 5-10% of an aloe extracting solution, 5-10% of a coix seed extracting solution, 3-5% of a portulaca oleracea extracting solution, 3-5% of VB3, 35-45% of ethyl alcohol, 6-10% of propylene glycol and the balance of water, wherein the sum of percentages by weight of the raw materials is 100%. According to the quick-drying type plant scar removal, black removal and restoration membrane, a continuous gas-permeable membrane having the property of positive ions is formed at the scars, so that the duration of stay of scar removing effective constituents is prolonged; the membrane has a certain pressure, so that effective constituents can easily permeate the skin, and the scar removing, black removal, restoration, antibacterial and itching relieving effects are obvious.

The invention discloses a Radix Puerariae tea and a preparation method thereof. The preparation method comprises the following steps: 1, cleaning wild Radix Puerariae, and removing skin and root; 2, cleaning and purifying Radix Puerariae skin and Radix Puerariae root, and drying them; 3, crushing into powder, and stirring and mixing the power, guar gum, xanthan gum and a proper amount of water to uniformity; 4, allowing the obtained material to stand, and carrying out granulation molding on the obtained material in a mixing granulator; 5, carrying out high temperature frying in a tea frying machine; 6, cooling, and sprinkling an aqueous solution of vitamin C to the obtained cooled material; and 7, carrying out low temperature vacuum drying and weighing packaging to obtain the Radix Puerariae tea. By optimizing a formula and a technology in the invention, the prepared Radix Puerariae tea which has a stable quality has the efficacies of heat clearing and alcohol effect relieving, blood fat reducing, blood pressure reducing, blood sugar reducing and the like. In addition, the preparation method which treats the Radix Puerariae skin and the Radix Puerariae root as raw materials provides technological supports for fully utilizing Radix Puerariae resources, increasing values of the Radix Puerariae tea and reducing wastes.

The invention relates to a compound amino acid capsule (8-11) and a preparation method thereof, specifically to a capsule including pellets of eight amino acids and eleven vitamins and a preparation method thereof. The capsule comprises 7 spherical pellets which are composed of the following raw materials by weight: 2.66 to 9.9 parts of a thiamine mononitrate pellet, 16.35 to 20.5 parts of a vitamin pellet a, 35.0 to 44.1 parts of an amino acid pellet a, 11.4 to 14.4 parts of a vitamin pellet b, 6.65 to 10.5 parts of an amino acid pellet b, 12. 73 to 16.0 parts of a vitamin C pellet and 5.7 to 9.36 parts of a vitamin AD bead. The capsule provided by the invention has the advantages of a scientific and reasonable ratio, good stability, no toxic and side effects, high security and good biological effects and can be applied in preparation of medicines used for treating chronic liver and kidney diseases and of foodstuffs used for alleviating and eliminating physical fatigue caused by exercise training. The capsule provided by the invention has characteristics superior to those of the prior art.

The invention discloses a method for improving the Pristinamycin (also named Peristamycin)output, in which the Pristinamycin is the fermentate of Streptomyces pristinaespiralis. By directly adding the macroreticular resinous adsorbent into fermentation liquor, the in-situ linkage of microorganism fermentation and product segregation can be carried out, which alleviates the Pristinamycin relaxation for production bacterium at a large degree and simultaneously avoids the probability that the Pristinamycin can be easily degraded in the later fermentation. By selecting macroreticular resinous adsorbent species, adding quantity, adding time and adding mode, the fermentation level of the said Pristinamycin can be increased to 1.0-2.5g / L, which can effectively improve the Pristinamycin output, accordingly it can reduce the Pristinamycin cost of manufacture.

Disclosed herein is a skin external composition for alleviating dry skin symptoms, which contains an extract of Scrophularia buergeriana Miq. as a main component and further contains an extract of Poria cocos Wolf, as well as the use thereof for skin-moisturizing cosmetics. The composition contains, as active ingredients, the Scrophularia buergeriana Miq. extract and the Poria cocos Wolf extract, which are prepared by extracting each of Scrophularia buergeriana Miq. and Poria cocos Wolf using water, ethanol, methanol, hexane, ethyl acetate or butanol.

The invention belongs to the technical field of animal feed processing and particularly relates to total mixed ration fodder for mutton sheep at the fattening stage. According to the total mixed ration fodder, alfalfa, corn, distillers' grains, soybean meal, malt sprout, corn fiber, cottonseed meal and molasses are mixed and smashed and then are sieved by a 40-mesh sieve, and mixed powder A is obtained; stone powder, calcium hydrophosphate, bentonite, baking soda, sodium chloride, sodium sulfate, montmorillonite, ammonium chloride, monensin, 0.5% of compound premix feed and the mixed powder A are put in a mixer to be mixed and conditioned, the conditioned material is granulated, granular fodder is obtained, packaged and graded, and a finished product is obtained. By comparison with a conventional feeding and fattening method, the marketing time of fattening lambs fed on the prepared fodder is advanced by 15-25 days, the mutton sheep averagely grow 300 g or more every day and the daily average feed intake is 2.5 jin in the process when the fattening lambs are fattened from 17.5 kg to 65 kg or higher, so that the cost of a breeding farmer is substantially reduced, and the benefits are remarkable.

The invention relates to a method for promoting lycopene biosynthesis in blakeslea trispora. The method comprises the following steps of: (1) respectively culturing blakeslea trispora (Blakeslea trispora) (+) (-) strains, and obtaining spore suspension; (2) respectively inoculating the blakeslea trispora (Blakeslea trispora) (+) (-) strains to conical flasks filled with a seed culture medium; (3) uniformly mixing the cultured positive bacteria seed solution in one bottle and negative bacteria seed solution in four bottles, and inoculating the seed solutions into a fermentation medium for fermenting; and (4) adding NAD<+> precursor substances within 24-48 hours after the beginning of the fermentation, and continuously culturing for 84 hours. The method is easy to operate, the yield of the lycopene is greatly improved, the production cost is reduced, and the method can be used for industrial production of lycopene.

The invention relates to the synthesis of polyketides in Actinomycetes by overexpression of pantothenate kinase and supplementation with pantothenic acid. This results in increasing in vivo CoA production and thereby drives increased production of secondary metabolites, such as polyketides.

The invention discloses a method for improving the content of mogroside III. The method comprises the following steps: inoculating siraitia grosvenorii tissue culture seedlings into a solid culture medium containing methyl jasmonate and carrying out tissue culture for 28 to 35 days, wherein the concentration of the methyl jasmonate is 50mu.mol / L to 400mu.mol / L. The method has the characteristics of rapid effect, low cost, simplicity in operation, convenience for implementation, no toxin and no residues of products and the like, so that large-scale production of the mogroside III is realized.

The invention discloses production of apple flavonoids by using a tissue culture method. The invention provides a method for preparing the flavonoids by using the tissue culture method, wherein the method comprises the following steps: 1) carrying out induction culture of an R6R6 genotype plant explant in a callus induction culture medium, to obtain a callus; 2) carrying out successive transfer culture of the callus in a successive transfer culture medium, to obtain a successive transfer callus; 3) carrying out enhancement culture of the successive transfer callus in an enhancement culture medium, to obtain an enhanced callus; and 4) extracting the flavonoids in the enhanced callus, to obtain the objective product. Experiments prove that the callus cultured by the method has the flavonoids content more than 30 times that of fruit pulp flavonoids of 'Jinshuai' and other apple varieties, and industrialized production of the apple flavonoids can be realized by using the tissue culture method.

The invention discloses a method for efficiently producing 1, 3-propylene glycol by reducing by-product acetic acid. According to the method, two genes, namely poxB and pta, in klebsiella pneumoniae are knocked out simultaneously, and the 1, 3-propylene glycol is produced by transforming glycerin more efficiently. The method has the advantages that after the two genes are knocked out, synthesis of a by-product, namely acetic acid, is reduced, and the efficiency of producing the 1, 3-propylene glycol by transforming the glycerin with the klebsiella pneumoniae is substantially improved.

The invention relates to mutant protein of a SgCS gene of momordica grosvenori, a nucleotide sequence coded by the gene, and application of the gene to production of mogroside V in a yeast host or a plant host.

The invention belongs to the field of fermentation engineering, and in particular relates to a method for improving the yield of amphotericin B produced through microbial fermentation. According to the method, by optimizing organic complex nitrogen sources and the fermentation conditions, the influences of the nitrogen sources of different batches on the fermentation metabolism are reduced; by adding precusor substances, the biosynthesis of the amphotericin B is promoted. After the fermentation is finished, the fermentation unit of the amphotericin B achieves 14469ug / ml. Compared with the result before optimization, the yield of the amphotericin B is improved by 103%, the fermentation time is shortened by 14%, the utilization rate of equipment is improved, and the purification of the downstream is benefited.

The invention discloses a Chinese traditional medicine health care product which can perfectly improve the sub-health condition and enhance the immune function to prevent diseases from happening and spreading. The medicine is made by the following materials: angelica, white paeony root, poria, bupleurum, atractylis, suncured ginseng, radix astragali, yam, Chinese magnoliavine fruit, haw, eucommia bark, dwarf lilyturf tuber, platycodon root and citrus aurantium. By combining the medicines, the invention coordinates the medicines so as to promote the overall function of enhancing the body immune function. The invention can strengthen health, benefit the heart and tonify the kidney, smooth the liver, nourish spleen and expel dampness, harmonize the stomach and lower the adverse flow, diffuse the lung and transform phlegm, invigorate qi and activate pulse, harmonize ying and wei, activate blood circulation and eliminate stasis, calm nerves and increase intelligence, and maintain the yin-yang balance of the internal body environment, so as to keep the qi and blood vigorous and the function of internal organs harmonious, thereby continuously improving the adaptability to the external environment and strengthening the reserve, resistance and resilience. The invention has advantages of no side effects, curing symptoms and root, advancing the viscera function, enhancing the immunity and improving the sub-health conditions.

The invention provides a novel application of thyroxine receptor analogues and pharmaceutically acceptable salts or predrugs thereof which are used for treating and / or preventing pulmonary diseases. The thyroxine receptor analogues and the pharmaceutically acceptable salts or predrugs thereof can effectively protect alveolar epithelial cells, adjust synthesis, storage and distribution of surface proteins of the alveolar epithelial cells, promote maturation, differentiation and integrity of the alveolar epithelial cells, are beneficial to elimination of hydrops in the lung, promote recovery of damaged mitochondria in the alveolar epithelial cells and enhance bio-synthesis of the mitochondria, so that repair of damaged lung tissue is facilitated, pulmonary fibrosis is stopped and reversed, immunoreaction is regulated, a cancerization environment is changed, pulmonary diseases are prevented and treated, pulmonary functions such as gas exchange and the like are improved, and the treatment effect is realized.

The invention discloses an application of fucoidan in preparation of intestinal prebiotics. The fucoidan has the functions of promoting carbohydrate metabolism, biosynthesis and metabolism of sugar, reducing membrane transport function, enhancing digestive system function or reducing disease infection, promoting production of short-chain fatty acids in intestinal tracts, improving intestinal microbial flora level. The fucoidan is used for preparing intestinal prebiotics, is used for human bodies or livestock and poultry animals, and has a wide application prospect.

The invention relates to Jiubaba Zengchanwang and belongs to an additive for cultivating edible fungi. The additive for cultivating the edible fungi comprises the following components in percentage by weight: 0.4 percent of nitrogen, 0.8 to 1 percent of phosphorus, 10 percent of amino acid, 2 percent of zinc, 4 percent of boron, 12 percent of calcium, 1 percent of magnesium and 0.3 percent of monopotassium phosphate, wherein the formula also comprises 0.008 to 0.01 percent of cobalt dioxide and the balance of various vitamin; and the weight sum of all the components is 100 percent. In the edible fungi cultivated by the additive, organic zinc and organic cobalt are absorbed by a human body easily. The edible fungi has effects of eliminating free radical, resisting inflammation, resisting tumor and resisting lipid oxidation, and contributes to enhancing disease resistance of the human body.

The invention relates to fish feed, in particular to mixed feed for onychostoma simum. The invention further relates to a preparing method of the mixed feed for onychostoma simum. The mixed feed for onychostoma simum comprises, by mass, 20-30 parts of fish meal, 8-15 parts of grass meal, 7-14 parts of cottonseed cake, 5-9 parts of bone meal, 6-10 parts of endothelium corneum gigeriae galli, 3-7 parts of ophiopogon japonicus, 3-7 parts of astragalus membranaceus, 2-5 parts of ligusticum wallichii, 2-6 parts of almond, 1-5 parts of dogbane leaf, 1-4 parts of water chestnut, 3-8 parts of tremella fuciformis, 2-6 parts of fish oil, 1-4 parts of honey, 1-4 parts of olive oil and 1-4 parts of compound vitamin. After the mixed feed for onychostoma simum is used for feeding, the coefficient of the feed for onychostoma simum is low, the crude protein content in the muscles of onychostoma simum is high, the crude fat content in the muscles of onychostoma simum is low, and the flavor amino acid content in the muscles of onychostoma simum is high.

The invention relates to a skin anti-ageing composition, in particular to a skin care product containing kinds of biological factors, and belongs to the fields of cosmetics and biotechnologies. Every10 ml of composition is composed of, by content, 5-40 mg of transparent hyaluronic acid, 50-400 mg of hydrolyzed collagen, 1-5 ml of mesenchymal stem cell secretion, 1-5 ng of EGF, 1-8 ng of b-FGF1 and 10 ml of sufficiently-supplemented purified water. The skin anti-ageing composition has the advantages that a formula is safe and effective, there are no allergic substances and preservative, ageingresistance and wrinkle removal are effective, the premature ageing of the skin is improved greatly, meanwhile, it is avoided that nutrition excess makes the skin burdened, and new skin problems appear, and the skin anti-ageing composition is particularly suitable for allergic skin.