6results about How to "Promote biosynthesis" patented technology

The present application relates to the field of bioengineering technology, and more particularly to the application of the amt gene and mutant strain M107 in expressing siderophore of nitrogen-fixing bacteria. The present application uses Tn5 transposition technology to construct a mutant library, and selects mutant strain M107 with significantly improved siderophore synthesis capacity compared with wild strain GXGL-4A. The insertion site of the mutant strain M107 is the aminomethyltransferase gene amt. Through CAS plate detection and relative content determination of siderophore, it is confirmed that the target mutant strain M107 has significantly improved siderophore yield compared with the wild strain, and the bacterial growth is not affected; the growth promotion experiment on cucumber shows that the obtained high-yield siderophore mutant strain has a significant growth promotion effect on cucumber. Compared with the prior art, the present application significantly improves the siderophore yield of the nitrogen-fixing bacteria by mutating the amt gene of the nitrogen-fixing bacteria, and the obtained mutant strain can be used as a plant growth promoting bacteria, which maintains the nitrogen fixation activity while increasing the absorption of iron in the soil.

This invention relates to the application and method of gold nanomaterials in improving the yield of polysaccharides and triterpenes, active products of Ganoderma lucidum, belonging to the field of microbial fermentation. The Ganoderma lucidum mycelial cells of this invention can produce triterpenes and polysaccharides through liquid fermentation. By adding a certain amount of gold nanomaterials to its liquid fermentation medium, the polysaccharide yield increased by 50.37% and the triterpenoid yield increased by approximately 42.78% compared to the state without gold nanomaterials, stably promoting the synthesis of polysaccharides and triterpenes. Simultaneously, this invention also provides the culture conditions for the fermentation synthesis of polysaccharides and triterpenes in Ganoderma lucidum under the induction of gold nanomaterials, providing a certain theoretical and practical basis for research on improving the biosynthesis of polysaccharides and triterpenes in Ganoderma lucidum.

This invention discloses a gene for rice resistance to Southern Rice Black-Streaked Dwarf Disease. OsWRKY30 Its application belongs to the fields of plant genetic engineering and molecular breeding technology. OsWRKY30 The gene encodes a WRKY family transcription factor, the nucleotide sequence of which is shown in SEQ ID NO:1, and the amino acid sequence of which is shown in SEQ ID NO:2. Experiments showed that knocking out this gene significantly reduced rice resistance to SRBSDV and exacerbated disease symptoms. This gene enhances rice's antiviral defense by positively regulating jasmonic acid biosynthesis and signaling pathways and activating the expression of a series of downstream disease resistance-related genes. Furthermore, in resistant and susceptible varieties... OsWRKY30 Natural variations associated with resistance were discovered in the promoter region, which can be used as molecular markers for disease resistance breeding. This invention provides important genetic resources and molecular tools for breeding new rice varieties resistant to SRBSDV.

The application discloses a gene of eudesmolsynthase AoTPS1 and an application of the gene and a coded protein, and provides a gene AoTPS1 for regulating biosynthesis of a monoterpenoid compound eudesmol, wherein a nucleotide sequence is shown as SEQ ID NO. 1, the total length of the sequence is 1785 bp, 594 amino acids are coded, the molecular weight is 69.03 kDa, and a coded amino acid sequence is shown as SEQ ID NO. 2. The eudesmolsynthase AoTPS1 protein has the function of catalyzing synthesis of eudesmol in vitro, meanwhile, overexpression of the eudesmolsynthase AoTPS1 gene by using a genetic transformation technology can significantly increase the content of eudesmol in monoterpenoid volatile oil compounds. Therefore, the AoTPS1 gene is a key gene for regulating synthesis of volatile oil of Eucommia ulmoides Oliv., and can significantly promote biosynthesis of eudesmol in monoterpenoid volatile oil and improve the medicinal value of volatile oil of Eucommia ulmoides Oliv.

The application discloses high trigonelline content porphyra haitanensis fermentation liquor and a preparation method and application thereof, and belongs to the technical field of food fermentation processing. The steps are as follows: (1) Pediococcus pentosaceus and Saccharomyces cerevisiae are respectively taken, activated, subcultured, centrifuged to collect the precipitate, washed and redissolved to obtain Pediococcus pentosaceus seed liquid and Saccharomyces cerevisiae seed liquid, mixed to obtain a fermentation inoculum; (2) the porphyra haitanensis is mixed with distilled water, homogenized, glucose is added, sterilized to obtain porphyra haitanensis slurry, the fermentation inoculum is added, fermented, and the supernatant is collected by centrifugation. By adopting the combined fermentation of Pediococcus pentosaceus and Saccharomyces cerevisiae on porphyra haitanensis, the synergistic metabolic effect of the co-culture system is utilized, the biological conversion efficiency and the enrichment amount of trigonelline are improved, and the prepared porphyra haitanensis fermentation liquor can significantly improve the body antioxidant capacity of a senescence model mouse, inhibit the expression of inflammatory factors and a senescence marker p16, and has significant antioxidant and anti-aging effects.

This invention provides a method for increasing the fermentation yield of beauveria bassiana, using a basal fermentation medium composed of 50-100 g / L carbon source, 10-30 g / L nitrogen source, and 5-25 g / L inorganic salts. During the 0-120 h fermentation period, a certain amount of L-phenylalanine and valine are added to the basal fermentation medium or during the fermentation process. This method can significantly increase the yield of beauveria bassiana, achieving a fermentation level of over 2900 µg / ml. Furthermore, the process is simple, stable, and suitable for industrial production.