14results about How to "Promote biosynthesis" patented technology

The present application relates to the field of bioengineering technology, and more particularly to the application of the amt gene and mutant strain M107 in expressing siderophore of nitrogen-fixing bacteria. The present application uses Tn5 transposition technology to construct a mutant library, and selects mutant strain M107 with significantly improved siderophore synthesis capacity compared with wild strain GXGL-4A. The insertion site of the mutant strain M107 is the aminomethyltransferase gene amt. Through CAS plate detection and relative content determination of siderophore, it is confirmed that the target mutant strain M107 has significantly improved siderophore yield compared with the wild strain, and the bacterial growth is not affected; the growth promotion experiment on cucumber shows that the obtained high-yield siderophore mutant strain has a significant growth promotion effect on cucumber. Compared with the prior art, the present application significantly improves the siderophore yield of the nitrogen-fixing bacteria by mutating the amt gene of the nitrogen-fixing bacteria, and the obtained mutant strain can be used as a plant growth promoting bacteria, which maintains the nitrogen fixation activity while increasing the absorption of iron in the soil.

This invention relates to the application and method of gold nanomaterials in improving the yield of polysaccharides and triterpenes, active products of Ganoderma lucidum, belonging to the field of microbial fermentation. The Ganoderma lucidum mycelial cells of this invention can produce triterpenes and polysaccharides through liquid fermentation. By adding a certain amount of gold nanomaterials to its liquid fermentation medium, the polysaccharide yield increased by 50.37% and the triterpenoid yield increased by approximately 42.78% compared to the state without gold nanomaterials, stably promoting the synthesis of polysaccharides and triterpenes. Simultaneously, this invention also provides the culture conditions for the fermentation synthesis of polysaccharides and triterpenes in Ganoderma lucidum under the induction of gold nanomaterials, providing a certain theoretical and practical basis for research on improving the biosynthesis of polysaccharides and triterpenes in Ganoderma lucidum.

The invention discloses a genetic engineering strain capable of solid-state fermentation and high yield of echinocandin B, and a construction method and application thereof, belonging to the technical field of genetic engineering bacteria. The engineering strain uses Aspergillus thunbergii as the base strain and uses a strong promoter. gpdA As ecdJ The promoter of ecdJ The invention provides a highly expressed engineered strain, thereby increasing the fermentation yield of echinocandin B; the constructed engineered strain is further fermented by solid fermentation, which saves costs and further increases the yield of echinocandin B. The invention strengthens the echinocandin B synthesis pathway by constructing an engineered strain, and combines the solid fermentation method to significantly increase the echinocandin B yield in Aspergillus thunbergii and realize solid fermentation, which has potential industrial application value.

This invention discloses a gene for rice resistance to Southern Rice Black-Streaked Dwarf Disease. OsWRKY30 Its application belongs to the fields of plant genetic engineering and molecular breeding technology. OsWRKY30 The gene encodes a WRKY family transcription factor, the nucleotide sequence of which is shown in SEQ ID NO:1, and the amino acid sequence of which is shown in SEQ ID NO:2. Experiments showed that knocking out this gene significantly reduced rice resistance to SRBSDV and exacerbated disease symptoms. This gene enhances rice's antiviral defense by positively regulating jasmonic acid biosynthesis and signaling pathways and activating the expression of a series of downstream disease resistance-related genes. Furthermore, in resistant and susceptible varieties... OsWRKY30 Natural variations associated with resistance were discovered in the promoter region, which can be used as molecular markers for disease resistance breeding. This invention provides important genetic resources and molecular tools for breeding new rice varieties resistant to SRBSDV.

The application discloses a gene of eudesmolsynthase AoTPS1 and an application of the gene and a coded protein, and provides a gene AoTPS1 for regulating biosynthesis of a monoterpenoid compound eudesmol, wherein a nucleotide sequence is shown as SEQ ID NO. 1, the total length of the sequence is 1785 bp, 594 amino acids are coded, the molecular weight is 69.03 kDa, and a coded amino acid sequence is shown as SEQ ID NO. 2. The eudesmolsynthase AoTPS1 protein has the function of catalyzing synthesis of eudesmol in vitro, meanwhile, overexpression of the eudesmolsynthase AoTPS1 gene by using a genetic transformation technology can significantly increase the content of eudesmol in monoterpenoid volatile oil compounds. Therefore, the AoTPS1 gene is a key gene for regulating synthesis of volatile oil of Eucommia ulmoides Oliv., and can significantly promote biosynthesis of eudesmol in monoterpenoid volatile oil and improve the medicinal value of volatile oil of Eucommia ulmoides Oliv.

The present invention discloses a palm leaf raspberry transcription factor RcWRKY33 and its application. The present invention provides a transcription factor RcWRKY33 that can promote the accumulation of plant total flavonoids and kaempferol-3-O-rutinoside, and is induced by hormones ABA, MeJA, and GA, and the nucleotide sequence is shown in SEQ ID NO.1. The transcription factor RcWRKY33 can bind to the W-box site (TTGACC) in the promoter region of the flavonoid synthesis pathway gene RcFLS1, activate the expression of the RcFLS1 gene, and then promote the biosynthesis of palm leaf raspberry total flavonoids and kaempferol-3-O-rutinoside. RcWRKY33 can significantly promote the biosynthesis of palm leaf raspberry total flavonoids and kaempferol-3-O-rutinoside, and improve the medicinal quality of palm leaf raspberry flavonoids and kaempferol-3-O-rutinoside.

The present invention discloses a high-expression nicotine chimeric gene, an expression vector and an application thereof, aiming to solve the technical problem of low nicotine biosynthesis efficiency in current tobacco. PMT1 The promoter contains the G-box element sequence "ATGCACGTTGTAAT", which realizes the regulation of the tobacco nicotine biosynthesis gene PMT, and the relevant chimeric genes and engineered bacteria are modified and designed. When applied to tobacco transformation, they can significantly increase the biosynthesis of tobacco nicotine.

The invention discloses a method for increasing the content of vinblastine in catharanthus roseus based on an EIN3 / EILs transcription factor CrEIL1 and a method for measuring the content of the vinblastine. The method comprises the following steps: cloning a Catharanthus roseus CrEIL1 gene, constructing a pLB-CrEIL1 plasmid vector, constructing a CrEIL1 overexpression vector by using the pLB-CrEIL1 plasmid vector, using the CrEIL1 overexpression vector, and performing agrobacterium tumefaciens-mediated overexpression of CrEIL1 in Catharanthus roseus, so that the yield of vinblastine in Catharanthus roseus can be increased in a low-cost, environment-friendly and reliable manner.

The invention discloses a Yarrowia lipolytica strain for increasing the yield of beta-carotene. On the basis of introducing a synthetic pathway, the production of beta-carotene is effectively enhanced by increasing the copy number of crtB, crtI, and crtY genes and overexpression of genes GGS1 and HMG related to the mevalonic acid (MVA) pathway, as well as the optimization of the promoter. The Yarrowia lipolytica strain of the invention accumulates about 507.7 mg / L beta-carotene in a shake flask culture, and the yield of beta-carotene obtained in a 5L fermenter reaches 5.5 g / L.

The application discloses high trigonelline content porphyra haitanensis fermentation liquor and a preparation method and application thereof, and belongs to the technical field of food fermentation processing. The steps are as follows: (1) Pediococcus pentosaceus and Saccharomyces cerevisiae are respectively taken, activated, subcultured, centrifuged to collect the precipitate, washed and redissolved to obtain Pediococcus pentosaceus seed liquid and Saccharomyces cerevisiae seed liquid, mixed to obtain a fermentation inoculum; (2) the porphyra haitanensis is mixed with distilled water, homogenized, glucose is added, sterilized to obtain porphyra haitanensis slurry, the fermentation inoculum is added, fermented, and the supernatant is collected by centrifugation. By adopting the combined fermentation of Pediococcus pentosaceus and Saccharomyces cerevisiae on porphyra haitanensis, the synergistic metabolic effect of the co-culture system is utilized, the biological conversion efficiency and the enrichment amount of trigonelline are improved, and the prepared porphyra haitanensis fermentation liquor can significantly improve the body antioxidant capacity of a senescence model mouse, inhibit the expression of inflammatory factors and a senescence marker p16, and has significant antioxidant and anti-aging effects.

The invention discloses a catharanthus roseus EIN3-EILs transcription factor and application thereof, and relates to the technical field of biology, the nucleotide sequence of the transcription factor CrEIN3 is shown as SEQ ID NO: 1, and the protein coding sequence is shown as SEQ ID NO: 2; the overexpression method comprises the following steps: cloning a catharanthus roseus CrEIN3 gene; constructing an overexpression vector; through agrobacterium tumefaciens mediation, an agrobacterium tumefaciens engineering strain is obtained; and instantaneously converting the Catharanthus roseus petals. The Catharanthus roseus EIN3-EILs transcription factor CrEIN3 is applied to increase of the content of vinblastine in Catharanthus roseus. The CrEIN3 is instantly overexpressed in the catharanthus roseus petals, so that the synthesis of the vinblastine in the catharanthus roseus petals can be remarkably improved, and the method has important significance for efficiently producing the vinblastine in the catharanthus roseus.

The invention discloses an eggplant bHLH family transcription factor SmbHLH93 protein and a coding gene thereof, the gene participates in regulating eggplant anthocyanin synthesis, the SmbHLH93 gene is overexpressed in wild-type arabidopsis thaliana, so that arabidopsis thaliana anthocyanin synthesis can be promoted, and a foundation is laid for improving plant quality by utilizing a genetic engineering technology in the future. A theoretical basis is provided for obtaining medicines or foods with high anthocyanin, and the method has a great application value.

The invention discloses a method for removing the bitter taste of Lichuanhong in summer and autumn. The method includes the steps of fresh leaf picking, light quality withering, leaf rolling conversion, air withering, rolling, fermentation, primary drying, rolling and frying, oxygen isolation maturation, drying, etc., wherein the light quality induction is yellow light quality withering of fresh leaves in a withering trough or an automatic withering machine; the leaf rolling conversion is to put the light withered leaves into the leaf rolling machine for rolling, and then put the light withered leaves that have been rolled into the green spreading trough for static conversion. During the static conversion process, red light is simultaneously irradiated to the light withered leaves in the green spreading trough; the air withering is to carry out air withering on the converted leaves in the withering trough or the automatic withering machine, and the red light is irradiated at the same time as the air withering; the fried leaves are not cooled, and directly enter the maturation machine for oxygen isolation maturation. The present invention removes the bitter taste of Lichuanhong in summer and autumn, improves the aroma of Lichuanhong in summer and autumn, has a high degree of process mechanization, stable tea making quality, simple operation, and low cost.

This invention provides a method for increasing the fermentation yield of beauveria bassiana, using a basal fermentation medium composed of 50-100 g / L carbon source, 10-30 g / L nitrogen source, and 5-25 g / L inorganic salts. During the 0-120 h fermentation period, a certain amount of L-phenylalanine and valine are added to the basal fermentation medium or during the fermentation process. This method can significantly increase the yield of beauveria bassiana, achieving a fermentation level of over 2900 µg / ml. Furthermore, the process is simple, stable, and suitable for industrial production.