59 results about "Tobacco nicotine" patented technology

An aerosol-generating rod comprises a gathered sheet of non-tobacco material circumscribed by a wrapper. The sheet of non-tobacco material comprises a sorbent substrate such as paper, a nicotine salt, and an aerosol-former. The sheet of non-tobacco material is textured or crimped An aerosol-generating article may comprise the aerosol-generating rod as a component element.

The invention provides a burley tobacco nicotine extract product and an extraction technology for the same. The burley tobacco nicotine extract product is composed of the following components according to weight by percentage: 94 to 97% of nicotine, 0.4 to 0.6% of cotinine, 0.2 to 0.3% of N-methy pridine carboxamide, 0.1 to 0.2% of benzofuran, 0.1 to 0.2% of indol, 0.1 to 0.2% of 5.5-dimethyle pyrazoline, 0.05 to 0.15% of acetyl pridine, 0.05 to 0.1% of megastigmatrienone, 0.02 to 0.05% of hydroxide radical damascenone, 0.02 to 0.05% of palmitic acid and 0.01 to 0.04% of demethylation nicotine. The burley tobacco nicotine extract product has nigh nicotine content and has special flavor when prepared for electronic tobacco juice; electronic cigarette satisfaction can be enhanced; and the electronic cigarette can be closer to cigarette taste.

The invention discloses a gene capable of reducing nicotine content of tobacco and an application thereof. The gene is a tobacco JAZ protein gene which is named as NtJAZ1, and protein encoded by the gene disclosed by the invention contains a typical TIFY structural domain and a jas structural domain and is induced and expressed by jasmonate. An RNAi (Ribonucleic Acid Interfere) transgenic carrier of the NtJAZ1 gene is constructed, and the tobacco is converted, so that expression of the NtJAZ1 in tobacco cells is successfully inhibited, and the nicotine content of the obtained transgenic tobacco cell line is obviously reduced compared with that of a wild type tobacco cell line. The gene disclosed by the invention provides an important basis and a technical support for low-nicotine-content tobacco breeding.

The invention discloses a gene capable of reducing nicotine content of tobacco and an application thereof. The gene is a tobacco JAZ protein gene which is named as NtJAZ3, and protein encoded by the gene disclosed by the invention contains a typical TIFY structural domain and a jas structural domain and is induced and expressed by jasmonate. An RNAi (Ribonucleic Acid Interfere) transgenic carrier of the NtJAZ3 gene is constructed, and the tobacco is converted, so that expression of the NtJAZ3 in tobacco cells is successfully inhibited, and the nicotine content of the obtained transgenic tobacco cell line is obviously reduced compared with that of a wild type tobacco cell line. The gene disclosed by the invention provides an important basis and a technical support for low-nicotine-content tobacco breeding.

The invention discloses genes capable of regulating and controlling nicotine content of a tobacco and application of the genes. A sequence of the genes capable of regulating and controlling the nicotine content of the tobacco is any one as shown in SEQ ID: No.1-21. The application of the genes is as follows: the genes capable of regulating and controlling the nicotine content of the tobacco are used for regulating synthesis and translocation amount of nicotine of the tobacco, so as to realize regulating and controlling of nicotine content in the tobacco. 21 genes obtained in the invention can form a stable hairpin structure, and analysis on the obtained tobacco non-coding small RNA sequence shows that part of small RNAs are from a sequence of a stem of the tobacco; and the small RNAs predict that target genes of the small RNAs are respectively targeted to a tobacco nicotine synthesis and transportation key protein coding gene. An miRNA regulating and controlling mechanism obtained in the invention can provide a deeper understanding for a role played by a key gene for a synthesis and translocation route of nicotine in tobacco, so that nicotine content character of the tobacco can be better improved.

The invention provides a method for extracting tobacco surface terpene and sugar ester compounds by utilizing tobacco land waste and application thereof. Fresh and untreated tobacco land production waste is used as a raw material, absolute ethyl alcohol is used as a solvent to extract an aroma component on the surface of the raw material, and a tobacco surface aroma component tincture containing terpene and sugar ester compounds is obtained. The terpene can be used for perfuming tobacco products. According to the extracting method, the raw material does not need to be pretreated, extraction isdirection performed by adopting the fresh raw material and the ethyl alcohol, the operation is simple, the cost is low, the raw material is not smashed, and the obtained extract contains few impurities. The remaining after extraction can be continuously used for extraction of beneficial active ingredients such as tobacco nicotine, chlorogenic acid and solanesol and can be also used for producingorganic fertilizers having insecticidal activity and containing nicotine, and staged high-value-added utilization of tobacco land waste can be achieved.

A guide tobacco smoke soda moves up transformation method and its application, its features are: the invention of the induction methods include the following steps: from the bottom 8-12 leaf tobacco plant leaves a bit, spraying concentration of 0.1% -10% of the 3, 5 - dinitrosalicylic acid in the environment temperature of 15 degree C -43 degree C under modulation processing time than four days to induce the conversion of nicotine into characters in the expression of green leaves in early. The induction method in tobacco breeding and seed breeding in the identification of nicotine into the application through the drop of nicotine and nicotine content, according to determine whether the conversion rate into line. The present invention has the advantage of being: May remain plants the warning from past example to decide on the smoke alkali to transform and to pick out ahead of time, achieves the purification seed's effect, may cut descendant's smoke alkali conversion rate effectively, regarding enhances the leaf tobacco fragrance and the security has the vital significance.

The invention discloses a whole genome selection model for predicting the nicotine content of tobacco and application thereof, and the whole genome selection model for predicting the nicotine contentof the tobacco is Bayes BNIC; in order that the prediction precision of the model on the phenotypic value of the nicotine content of the tobacco is optimal, core parameter values such as the number (n1) of molecular markers of the candidate prediction model Bayes B, the scale (n2) of a training group, the ratio (n3) of the training group to a test group and a model prediction precision value (n4)are clearly stipulated. The application refers to the application of the whole genome selection model Bayes BNIC to analyze the genotype data of a tobacco group so as to predict the nicotine content of the tobacco group. The tobacco nicotine content whole genome selection model Bayes BNIC provided by the invention can accurately predict the nicotine content value of each plant in a tobacco group according to the genotype of the tobacco group, so as to realize the cultivation of excellent tobacco varieties (lines) with different nicotine content levels in tobacco quality breeding.

The invention discloses a microbial strain and an application thereof, particularly relates to a tobacco nicotine degrading microbacterium GYC29 (Microbacterium sp.GYC29) with CCTCC of NO:M2010311 and an application of the microbacterium in tobacco. The strain disclosed herein can decompose and utilize nicotine in the growth process. According to the invention, by adding the ferment liquor of the strain or thallus with the additional amount of 1-5 % of the weight of the tobacco leaves in the tobacco with the water content of 10-50 % and fermenting for 6-72 h, the nicotine content of the tobacco leaves is reduced by 2-33%, the irritation of the tobacco leaves is obviously reduced, the miscellaneous gases are reduced, the smoke becomes pure and mild, and the sensory quality is obviously improved; the degradation of tobacco nicotine is realized by using microbes, the nicotine content of tobacco raw materials can be regulated properly, and the applicability of the tobacco leaves is raised.

The invention discloses a tobacco nicotine content regulation gene IAA27 and a cloning method and application thereof. The nucleotide sequence of the tobacco nicotine content regulation gene IAA27 isshown in SEQ ID NO: 1; The amino acid sequence of the encoding protein is shown in SEQ ID NO: 2. The cloning method comprises synthesizing tobacco leaf cDNA, extracting total RNA from tobacco leaf, and reversing transcribing to obtain first strand cDNA; performing PCR amplification of IAA27 gene; designing primers according to the IAA27 gene sequence, with the tobacco leaf cDNA being adopted as atemplate; performing PCR amplification; recovering and purifying a PCR amplification product; and sequencing the product. The tobacco nicotine content regulating gene IAA27 is used for obtaining transgenic tobacco plants with high nicotine content. Inhibition of the expression of IAA27 gene in tobacco plants can obviously improve the nicotine content in tobacco leaves, and has wide application prospect in actual production.

The invention relates to the field of tobacco planting, and particularly discloses a method for adjusting and controlling nicotine content of tobacco. The nicotine content of the tobacco is adjusted and controlled by combining grafting with the means of adjusting and controlling transplanting time and the ridging height. The overground part of the tobacco is grafted to a Solanum torvum rootstock with no nicotine synthesis ability, tobacco plants with reconstructed root systems are obtained, the occurrence amount of adventitious roots is artificially controlled according to different ridging heights, and the purpose of adjusting and controlling the nicotine synthesis amount and flue-cured tobacco nicotine content is achieved by controlling nicotine synthesis sources (root systems). The grafting combination affinity is good, the grafting survival rate is high and reaches 95% or above, the grafted tobacco plants normally grow and have no remarkable difference compared with own-rooted tobacco plants, nutrient absorption is normal, and other quality indexes of flue-cured tobacco leaves are reserved while low nicotine content is achieved.

The invention discloses a flue-cured tobacco nicotine content reducing method; topping is done in a period between tobacco strain bud immersion and center flower opening; in topping, 2-3 stipules under the grandiflora are also removed, so 16-20 tobacco strain effective leaves are remained; a reagent having naphthalene acetic acid is immediately coated on a stem section after the topping, then a rubber finger sleeves on the stem top so as to prevent medicament loss or rain washing; in addition, a bud-control compound is applied to control side buds. The method can effectively reduce flue-cured tobacco nicotine content, improves tobacco leaf chemical component coordination property, is simple, and good for popularization application.

The invention discloses co-dominant SSR (simple sequence repeat) markers closely interlinked with a major regulation and control gene nic1 of nicotine synthesis of nicotiana tabacum and application ofthe co-dominant SSR markers. The serial numbers of the co-dominant SSR markers closely interlinked with the major regulation and control gene nic1 of nicotine synthesis of nicotiana tabacum are TM22038, TM23004 and TM22041, and nucleotide sequences of PCR (polymerase chain reaction) amplification products of the markers are respectively shown in SEQ ID No.1, SEQ ID No.2 and SEQ ID No.3 and SEQ IDNo.4, SEQ ID No.5 and SEQ ID No.6. The application refers to application of the co-dominant SSR markers closely interlinked with the major regulation and control gene nic1 of nicotine synthesis of nicotiana tabacum in detecting whether nicotiana tabacum genome DNA (deoxyribonucleic acid) has a homozygous genotype nic1 or not and a nicotiana tabacum variety of a low nicotine content is available or not. The co-dominant SSR markers disclosed by the invention have the characteristics of being stable, reliable, simple and convenient, rapid and low in cost, so that the molecular markers can be applied to assistant selection of molecular markers of the low nicotine content gene nic1 in high-quality nicotiana tabacum breeding.

The invention relates to the field of genetic technologies and botany, and aims at providing a tobacco nicotine synthesis related long non-coding RNA gene and application of the tobacco nicotine synthesis related long non-coding RNA gene. The sequence of the gene is SEQID NO:1. The gene is used for adjusting the tobacco nicotine resultant quantity so as to adjust and control the nicotine content in tobaccos. A genetic mechanism of adjusting and controlling nicotine synthesis by degrading miRNA(Nta-miRNAX27a) of the target nicotine synthesis key gene (QPT2) through the tobacco eTM gene (Nta-eTM27) is disclosed. According to the eTM adjusting and controlling mechanism, a more-deep comprehension is provided for a role played by a key gene in the tobacco nicotine synthesis way, and the tobacco nicotine content character can be better improved conveniently.

The invention discloses a new member NtIRL2 of a Nicotiana tabacum isoflavone reductase-like (NtIRL) gene family. The sequence of an open reading frame of the NtIRL2 is shown as 79 nucleotide to 1011 nucleotide in SEQ ID NO.10. The invention performs systemic bioinformatic analysis on the members NtIRL1 and NtIRL2 of the NtIRL gene family, sets forth the expression features of the NtIRL1 and NtIRL2 genes in each tissue and organ of Nicotiana tabacum, constructs a plus sense expression vector and an antisense expression vector, and verifies that the NtIRL1 and NtIRL2 genes are coded with activated nicotine synthesis correlated enzymes through genetic transformation. The NtIRL1 and NtIRL2 genes play vital roles in the biosynthesis and metabolic pathway of tobacco nicotine, participate in the fungal disease defense of tobacco plants, can be used for regulating the nicotine content of Nicotiana tabacum and improving the disease resistance, and is beneficial to the gene engineering breeding of high-quality tobacco having low nicotine content and high fungal disease resistance.

The invention discloses a drought-resistant agent for flue-cured tobacco. The drought-resistant agent for the flue-cured tobacco is a product prepared by the following steps of: preparing mother liquor from auxin and abscisic acid serving as basic raw materials, mixing the mother liquor with Tween to obtain mixed liquor, and mixing the mixed liquor with molten wool grease uniformly, wherein the content of the auxin is 98 to 99 percent, and the content of the abscisic acid is 10 percent; the mass fraction of the mother liquor prepared from the auxin and the abscisic acid is 1 to 5 percent; the quality index of the Tween is that the water content is less than or equal to 3 percent; the mass ratio of the mother liquor to the Tween is (1-2 percent):(98-99 percent); the molten wool grease is prepared by heating and melting at the temperature of between 60 and 80 DEG C and the relative humidity of more than or equal to 80 percent; and the mass ratio of the mixed liquor to the molten wool grease is (0.5-2 percent):(98-99.5 percent). After the drought-resistant agent is coated on the cross section of the top of a stem after the topping of a tobacco strain, the drought resistance of tobacco at the late growth stage can be improved, the content of tobacco potassium can be improved, and the content of tobacco nicotine can be reduced; and the drought-resistant agent has the advantages that: the consumption and cost are low, the operation is simple and convenient, key technical points are easy to grasp, and the drought-resistant agent has high applicability and multiple functions.

The invention belongs to the technical field of genetic engineering, particularly relates to a method for increasing nicotine content in tobacco by utilizing camptotheca acuminate AOC (Allene Oxide Cyclase) gene transformation. In the method, a plant expression vector is constructed by using camptotheca acuminate allene oxide cyclase (AOC) genes; and the nicotine content in the tobacco is increased through over-expression of the camptotheca acuminate AOC genes in the tobacco by using a genetic engineering technology. The method comprises the process of: cloning the AOC genes from camptotheca acuminate; constructing a plant high-efficiency expression vector; introducing agrobacterium tumefaciens and performing genetic transformation on the tobacco; and measuring the nicotine content in transgenic tobacco leaves. By adopting the method, the camptotheca acuminate AOC genes can be over-expressed in the tobacco, and the nicotine content in the tobacco is increased.

The invention discloses a tobacco JAZ protein gene and application thereof in reducing tobacco nicotine content. The gene is a tobacco JAZ protein gene and is named as NtJAZ7, the protein coded with the gene has a typical TIFY structure field and a jas structure field, and the gene is subjected to induced expression of jasmonate. Through constructing an RNAi gene transfer vector of the NtJAZ7 gene, and transforming the tobacco, the expression of NtJAZ7 in tobacco cells is successively inhibited, and the nicotine content of the obtained transgenic tobacco cell line is obviously reduced compared with the wild type tobacco. The invention provides an important scientific basis and a technological support for breeding low-nicotine content tobacco.

The invention discloses a tobacco nicotine content regulation gene NtCLC-b. The gene is capable of encoding a polypeptide for regulating the tobacco nicotine content. An amino acid sequence contained in the polypeptide is shown as SEQ ID: No.2; a nucleotide sequence contained in the tobacco nicotine content regulation gene NtCLC-b is shown as SEQ ID: No.1. The invention further discloses a cloning method and application of the regulation gene NtCLC-b. Due to over-expression of the gene NtCLC-b in tobacco plants, the content of nicotine in tobacco can be obviously reduced, and the tobacco nicotine content regulation gene has wide application prospects in actual production.

The invention relates to a method for predicting the release amount of phenol in mainstream smoke of flue-cured tobaccos. The method comprises the following steps of: performing sample pretreatment on the flue-cured tobaccos to be tested through manual tearing, stem removal, shredding; detecting eight chemical components comprising linoleic acid, total sugar, total nitrogen, chlorogenic acid, malonic acid, total polyphenol, tobacco nicotine and volatile acid of a sample to be tested; calculating network values of thirteen nodes of a hidden layer by combining the measurement results of the eight chemical components with the coefficients of each input layer of a model; converting the network values of the thirteen nodes of the hidden layer into the output values of the thirteen nodes of the hidden layer; and calculating to obtain a predicted value of the release amount of the tobacco phenol by combining the output values of the thirteen nodes of the hidden layer with the coefficients of the output layers of the model. According to the method, the content of phenol in the smoke can be predicted through the model, cigarette rolling and smoking machine equipment suction are not required to capture the particle phase substances; and moreover, the accumulated content of possible phenol in the finished cigarette products to be formed in the future can be effectively predicted through the flue-cured tobacco raw materials, so that raw material selection in the production process is guided.

The invention discloses a method for reducing burley tobacco nicotine, which comprises the following steps: selecting a tomato seedling as a stock and a burley tobacco seedling as a cion, grafting by adopting a cleft graft method, horizontally cutting off the upper half part of the stock that is 8 cm to 10 cm from the soil surface, and vertically downwards cutting a cut with the length of 1 cm; cutting off the upper half part of the cion from a parent, grinding two sides of a stem into wedge-shaped structures with the length of 1 cm, vertically inserting the wedge-shaped structure into the stock, fixing by a grafting clip, and moving into a coalescence curing chamber. The synthesis of the burley tobacco rooted nicotine is controlled manually through a grafting manner, the burley tobacco nicotine is reduced remarkably, and the problem that the content of the burley tobacco nicotine is too high is solved effectively. As burley tobacco and tomatoes are classified as solanaceae, the burley tobacco and the tomato have good affinities, and the grafting success rate reaches more than 70%. The content of nicotine of the burley tobacco and tomato combination is reduced remarkably, the field growth condition is basically consistent with the cion own-rooted seedling, and the acre yield remains almost the same.