Breeding method capable of reducing conversion rate of tobacco nicotine

Abstract

The invention provides a breeding method capable of reducing the conversion rate of tobacco nicotine. The breeding method is characterized in that the advantages of the first generation of the male sterile hybrid are utilized, the materials comprise male sterile female parent and a maintainer line as well as male parent, the tobacco nicotine conversion rates of the male sterile hybrid female parent and the maintainer line as well as the male parent need to be smaller than 5%, the tobacco nicotine conversion rates of the male sterile hybrid female parent and the maintainer line need to be smaller than the tobacco nicotine conversion rate of the male parent; the male sterile female parent and maintainer line and the male parent with the tobacco nicotine conversion rates higher than 5% need to be subjected to selection and purification of the tobacco nicotine; the selection and purification of the tobacco nicotine of the male sterile hybrid female parent with the tobacco nicotine conversion rate higher than 5% comprise the synchronous purification of the male sterile female parent and the maintainer line. According to the method, the tobacco nicotine conversion rate character has negative hybrid advantage in the hybridization process, namely, the tobacco content of the hybrid F1 is more than a parent average value, the nornicotine is greatly lower than the parent average value, the parent with lowest nicotine conversion rate is taken as a hybrid female parent, the hybrid advantage is maximum, the operation is simple, and the effect is obvious.

Description

technical field [0001] The invention belongs to the field of tobacco breeding, and in particular relates to a breeding method suitable for utilizing male sterile first-generation heterosis for reducing the conversion rate of tobacco nicotine. Background technique [0002] Tobacco alkaloids mainly include nicotine, nornicotine and trace amounts of anonicotinoid and pseudobasine. Among them, nicotine is the main alkaloid of common tobacco varieties, with a content of more than 92%; nornicotine is mainly formed by demethylation of nicotine directly catalyzed by demethylase, so nornicotine is also called demethylnicotine, This demethylation ability of nicotine is the nicotine conversion ability. Under normal circumstances, the conversion of nicotine to nornicotine in common tobacco is controlled by the homozygous recessive genotype, which does not have the ability to demethylate nicotine, so the content of nornicotine generally does not exceed 3.5%. However, in the tobacco pla...

AI Technical Summary

Problems solved by technology

That is to say, it is impossible to breed varieties with low nicotine conversion rate and low nornicotine content by adopting the method of male sterile cross generation advantage utilization

At the same time, the nicotine conversion traits are different from the traits that can be directly judged by measuring tools or human senses, such as leaf size, color depth, yield, etc., and must be tested indoors after tobacco leaf modulation or after fresh leaves have been artificially induced. We know that, in addition, nicotine conversion is caused by gene mutation, so there is greater uncertainty and instability in genetics, which increases the difficulty of breeding

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