351 results about "Sterility" patented technology

A closure system for a medication container, the interior of which is accessible via a container opening designed in the manner of a bottle mouth, comprising a bump cap with an annular lid having a central opening, on the outer circumference of which is arranged a number of latching elements which can be brought into engagement with an outer bead mounted circumferentially on the container opening, and a one-piece closure plug with a closure body which completely fills the central opening of the annular lid and can be brought into latching engagement therewith, is intended to enable continuous compliance with even high sterility requirements during handling in a simple and reliable manner. For this purpose, the closure body is designed as a duckbill valve.

The invention discloses a male sterility gene ZmUCE4 and application thereof in creating a male sterility line of corn, and belongs to the field of plant biotechnology breeding. According to the invention, site-directed mutagenesis of the ZmUCE4 gene in wild type corn is carried out through a CRISPR / Cas9 gene editing technology, pollen development abnormality and abortion can be caused, complete male sterility is caused, and it is proved that the ZmUCE4 gene has a regulation and control function on corn male reproductive development. Through offspring screening, a sterile line without transgenic components can be obtained, a stable maize male sterile line is created, and the method has important significance on maize male fertility control and hybrid seed production. The invention also designs a functional molecular marker aiming at the obtained uce4 male sterility mutant gene, and has important application value in maize male sterility line cultivation, sterile hybrid seed production and molecular marker-assisted selection.

The invention belongs to the technical field of biology, and particularly relates to a Yu-grain rpl16 gene and application thereof, in particular to application of the rpl16 gene in cultivation of a rice sterile line. Overexpression of the Yu-grain rpl16 gene in rice can cause rice pollen abortion, so that rice male sterility is realized, the breeding period is shortened, the breeding efficiency is improved, and theoretical guidance is provided for culturing a new male sterility line.

The invention provides an integrated storage and sterilization device for multi-specification cell culture apparatuses, and belongs to the technical field of crossing of biomedical engineering and laboratory intelligent equipment. The device comprises a multi-specification cell culture appliance information acquisition module, a sterilization parameter intelligent calculation module, a sterilization process execution and real-time monitoring module, a sterilization effect intelligent judgment module and a multi-specification cell culture appliance storage adaptation module. According to the invention, through integration of an integrated architecture and an algorithm, sterile treatment, intelligent management and full-process tracing of multi-specification cell culture apparatuses are finally realized, high requirements of biomedical experiments on sterility, adaptability and controllability of the apparatuses are met, meanwhile, the operation efficiency of a laboratory is improved, and personal errors and pollution risks are reduced.

The invention discloses a freeze-drying automatic conveying system, which relates to the technical field of freeze-drying equipment, and comprises a conveying track which penetrates through a freeze dryer, and the surface of the conveying track is covered with an anti-frosting nano coating. According to the invention, a conveying track covered with an anti-frosting nano coating, an intelligent tray embedded with an RFID chip and a temperature sensor, a transport vehicle carrying visual monitoring, and a bipolar transition cavity module equipped with an electric translation air-tight door and having environment control and sterilization functions are integrated and coordinated by a central controller; full-process automatic and intelligent conveying of materials among freeze-drying pre-treatment, core freeze-drying and post-treatment areas is achieved, then accurate real-time monitoring and full-process tracking of core process parameters are guaranteed, a sterile, low-oxygen and low-humidity environment is rapidly established through an efficient two-stage transition cavity, the pollution risk is reduced, the sterility and stability of products are guaranteed, and the product quality is improved. Therefore, the product quality consistency, the production efficiency and the equipment utilization rate are improved, and the labor cost and the operation risk are reduced.

The invention relates to the technical field of intelligent manufacturing, in particular to sterile directly-prepared blowing-filling-sealing-time-throwing-bottle eye washing liquid and a preparation process thereof. The process comprises the following steps: S1, preparing materials; s2, concentrated preparation; s3, diluting and preparing; s4, sterilizing and filtering; and S5, through a BFS sterile filling process, injecting the to-be-filled eyedrops in the liquid storage tank while performing blow molding on the secondary throwing bottle by using sterilized sterile air, and sealing to obtain the secondary throwing bottle eyedrops. The eye washing liquid in the secondary throwing bottle is prepared from directly prepared eye washing liquid materials in a sterile manner; a multi-stage filtration sterilization and storage time threshold early warning discarding process is adopted in the preparation process, so that the sterile condition in the preparation process is ensured; and meanwhile, a BFS sterile filling process is adopted, so that blowing, filling and sealing are integrated, and sterility is realized.

The invention relates to a male gamete killer gene S19g-A1 of a rice interspecific hybrid sterile S19 seat and application of the male gamete killer gene S19g-A1. The invention provides a rice hybrid sterility related protein S19g-A1. The amino acid sequence of the rice hybrid sterility related protein S19g-A1 is as shown in SEQ ID NO. 2. According to the invention, a key gene S19g-A1 of a rice interspecific hybrid sterile S19 site is researched and identified, function knockout is carried out on African rice S19g-A1, and pollen fertility of a mutant hybrid F1 obtained by carrying out distant hybridization on an obtained African rice s19g-a1 mutant returns to normal. Based on a gene editing technology, the invention develops a method for rapidly creating a hybrid affinity line capable of overcoming sterility of African rice hybrid, effectively eliminates S19 locus mediated interspecific hybrid reproductive disorder, breaks through the bottleneck of rice interspecific hybrid advantage utilization, provides target gene resources for distant hybrid advantage utilization and crop yield improvement, and has a wide application prospect. Good application prospects are realized.

The invention discloses a gene of a rice indica-japonica intersubspecific hybrid sterile locus HS12 and application thereof, and belongs to the field of plant genetic engineering. The locus is positioned at the tail end of a long arm of a chromosome 12, is positioned between molecular markers Sy55 and Sy8, and comprises four fertility related genes iTx1, iTx2, iAd1 and jAd2. By functional knockout or inhibition of expression of the gene iTx1 and / or iTx2, iAd1 and / or jAd2 are / is transferred, a wide-compatibility rice material can be created, meanwhile, a molecular marker for detecting HS12 is further provided and used for filial generation detection and auxiliary breeding, indica-japonica hybrid sterility can be effectively overcome, and the setting rate of offspring is increased.

The invention discloses a ZaSCL3 gene for regulating and controlling plant growth and development as well as an encoding protein and application thereof, and belongs to the technical field of gene engineering. According to the invention, an over-expression vector of the ZaSCL3 gene is constructed and expressed in a wild tomato, so that stamens of a ZaSCL3 over-expression plant are aborted, ovary is spontaneously expanded without pollination, and seedless fruits are generated, which indicates that the over-expression ZaSCL3 gene can realize the effect of inducing male sterility and apomixis of the plant; a key gene resource is provided for molecular breeding and yield character improvement, and the important application value is achieved.

The invention discloses a lettuce LsNP1 gene and application of the lettuce LsNP1 gene in preparation of male sterile plants. Specifically, the invention discloses a protein LsNP1 with an amino acid sequence of SEQ ID NO: 1 and application of a coding gene of the protein LsNP1 in forming a male sterility phenotype of lettuce. Experiments show that the number of pollen grains of the mutant obtained after the LsNP1 gene is knocked out is obviously reduced, the pollen activity is obviously reduced, the pollen grains are abnormal in shape, and anther cannot normally scatter and inseminate during flowering, so that lettuce forms a male sterility phenotype. The male sterile lettuce is successfully created, valuable gene resources are provided for germplasm resource innovation and genetic improvement of the lettuce, a new field is developed for application of the LsNP1 gene, and a new material is provided for application of a lettuce male sterile line. By utilizing the gene and the method, the lettuce male sterile line germplasm can be rapidly created, the cultivation process of a new lettuce variety is accelerated, and the gene and the method have a wide application prospect in the field of agricultural production.

The invention relates to the technical field of plant breeding, in particular to a method for constructing a dominant male sterile line based on CIF1 gene overexpression. The brand-new dominant male sterility gene CIF1 is found, it is verified that the gene has functions in monocotyledonous and dicotyledonous plants, and dominant male sterility resources are enriched; besides, a method capable of manually controlling the fertility recovery of the dominant sterile line is found, a reproductive breeding line for large-scale propagation is obtained, male sterile filial generations can be obtained by hybridizing the breeding line with normal plants, and a new application way is provided for large-scale application of dominant sterility.

Injectable compositions and methods of preparation, as well as therapeutic uses, of induced pluripotent stem cell (iPSC)-derived notochordal cell (iNC)-loaded microgels are provided. Microfluidic on-chip platform can be utilized to prepare microgels (or microgel particles / spheres) formed from block copolymers that exhibit reverse thermal gelation, so as to encapsulate iNCs. Also provided are preconditioned iNC-loaded microgels and iNCs in bulk hydrogel. Cell purity, identity, viability, sterility, and the stability of microencapsulated iNCs have been evaluated. Safety and efficacy of the compositions as therapeutic candidates has been tested via intradiscal injection in animal models of intervertebral disc (IVD) degeneration and discogenic low back pain. Biobehavioral testing, MRI, and immunohistochemical analyses were utilized to evaluate the regenerative potential and reproducibility of the compositions as therapeutic candidate. Single cell RNA sequencing of the treated IVDs may also reveal mechanism of action of the compositions.

The present invention relates to a liquid dispenser with a tip opening mechanism of the type without preservatives for blow-fill-seal containers that comprises a dual function membrane to control the passage of air and liquid and a pierceable silicone seal that ensures sterility at all times when in the storage position and when opening the container.

The present invention discloses a preparation method of an active injectable gel that can withstand irradiation sterilization. The active injectable gel is composed of a collagen gel extracted from an animal source, acellular dermal matrix microparticles, bacterial cellulose, glycerol, and amino acids compounded in a certain proportion, and serves as a filling material that plays an exogenous filling and endogenous collagen regeneration role. Through the protection of glycerol and amino acids and the method of cryopackaging with liquid nitrogen or dry ice, the collagen gel can still maintain its activity and gel properties after being sterilized by Co60 irradiation, and achieve the effects of sterility and virus inactivation. By adding acellular dermal matrix microparticles and bacterial cellulose, the injection force is increased and the action time of the pure collagen gel is effectively extended to achieve a long-term effect.

The invention discloses a KASP molecular marker for identifying a Chinese cabbage nuclear male sterility gene and application of the KASP molecular marker. The invention belongs to the field of molecular biology, and particularly relates to a KASP molecular marker for identifying a Chinese cabbage nuclear male sterility gene and application of the KASP molecular marker. The method for identifying or assisting in identifying the Chinese cabbage fertility comprises the steps that the genotype of an SNP site in a Chinese cabbage genome to be detected is detected, the Chinese cabbage fertility is identified or assisting in identifying according to the genotype, the SNP site is an SNP site on a Chinese cabbage A07 chromosome, the nucleotide type of the SNP site is C or T, and the SNP site is the 1935th nucleotide of SEQ ID No: 4 in a sequence table. The SNP locus related marker can be used for Chinese cabbage male sterility molecular marker assisted breeding, and a new gene resource is provided for Chinese cabbage heterosis utilization.

The invention belongs to the field of plant biotechnology breeding, and relates to an application of a ZmGalAT1 gene and an encoding protein thereof in corn fertility control. According to the invention, a corn male sterility mutant galat1 is obtained by EMS mutagenesis, and a gene ZmGalAT1 for controlling corn male flower development is identified through map-based cloning. The gene is subjected to site-directed mutagenesis in wild type corn through a CRISPR / Cas9 gene editing technology, three galat1 allelic corn male sterility mutants are created, corn male flowers of the mutants are completely aborted, sterile mutants without transgenic ingredients can be obtained through offspring screening, and the mutants are further applied to corn sterile breeding and seed production. The invention also designs functional molecular markers for three allelic male sterile mutants of GalAT1, and has important application value in maize male sterile line cultivation, sterile hybrid seed production and molecular marker-assisted selection.

This invention belongs to the field of molecular genetics and discloses a method and application for controlling fish fertility using in vivo gene editing technology. The applicant, for the first time, cloned a carp-specific U6 promoter and used this promoter to construct the U6 gene. MOVIE gRNA transgenic vectors and water The Cas9 vector allows for the establishment of an in vivo editing system. This strategy enables the heritability of sterility, a reproductive control trait, through hybridization of fertile parents to produce sterile offspring. The operation is highly efficient, simple, and environmentally friendly. Furthermore, since high-copy-rate short, scattered repetitive sequences exist in all fish species, this strategy has broad applicability across species.

According to the analyte detection device installation unit with the sterile barrier, before installation is carried out, the subcutaneous penetrating part of an auxiliary needle is contained in a needle body containing cavity of an outer cover body, a main sterile barrier is arranged at the contact position of the outer cover body and a first through hole, and therefore after the subcutaneous penetrating part is subjected to directional irradiation sterilization, the auxiliary needle can penetrate through the needle body containing cavity of the outer cover body; the subcutaneous piercing part is located in a closed sterile environment, germs existing in the unsterilized part in the installation unit cannot enter the sterile environment, the subcutaneous piercing part cannot carry germs again after directional sterilization, health of a user cannot be affected, and use safety of the user is guaranteed.

The invention provides a method for rapidly inducing calluses and proliferating by utilizing blackberry lily seeds. According to the method, the callus is directly induced by the blackberry lily seeds for the first time, the induction time is short, the callus can be induced after the blackberry lily seeds are used as explants and disinfected for 25 days, and the induction rate reaches 91.75%. After the seeds are sterilized for 5 days, the sterility rate is 56.96%, the sterility rate in the callus induction period is 94.86%, and the sterility rate in the callus subculture period is 100%. The callus is high in growth speed and good in activity, the proliferation rate of 3-4 mm callus mass is 93.23%, the browning rate of the callus during subculture is only 7.92%, and the callus can be proliferated for a long time. The method is easy to master, low in cost and high in efficiency, materials are easy to obtain, the method is not limited by seasons, a large number of blackberry lily calluses can be provided in a short time, and a foundation is laid for follow-up establishment of a blackberry lily efficient improved variety propagation system and a genetic transformation system.

The invention provides a capsicum annuum cell nucleus male fertility gene Msc-4, a co-separation molecular marker of the gene and application of the capsicum annuum cell nucleus male fertility gene Msc-4. The amino acid sequence of the protein coded by the gene is as shown in SEQ ID NO. 1. The invention further provides a molecular marker SNP-31372133 co-separated with the gene Msc-4, and the molecular marker SNP-31372133 can assist in screening of a hot pepper material with nuclear male sterility. The invention provides a new nucleus sterile germplasm resource and a new nucleus male sterility gene resource for the research on the nucleus male sterility of the pepper, and plays an important role in the application in the field of pepper seed production and breeding.

The invention relates to the technical field of forest cultivation, and discloses a carya illinoensis fruit forest variety configuration method which comprises the following steps: configuring a main cultivation variety and a pollination variety in a fruit forest; preparing a pollination synergistic composition; the composition is sprayed at the initial stage of stigma exposure of female flowers of a main cultivated variety. The composition comprises a phenolic acid grafted carboxymethyl chitosan derivative, low methoxyl pectin, a calcium ion cross-linking agent, sugar alcohol chelated boron and spermidine. According to the method, an interfacial film with antioxidant activity is formed on the surface of the stigma by using the grafting modified matrix, so that the stigma browning is effectively inhibited, and the pollination period is prolonged; by constructing a calcium cross-linked low-methoxyl pectin interpenetrating network, an interfacial film is endowed with an intelligent degradation characteristic of enzyme-sensitive response to pollen, and protection at ordinary times and immediate dissolution after touching flowers are realized. According to the method, pollen is captured through static electricity, nutritional factors are released in a targeted mode, the pollination and fertilization efficiency is remarkably improved, the sterility risk in the flowering phase is relieved, and the yield and quality of carya illinoensis are improved.

Various packaging assemblies and methods for preserving sterility of a sensing device prior to insertion into a body cavity of a subject are described herein. Some implementations of a packaging assembly include: a bag comprising an interior, the bag at least partially comprising a transparent material; and a tray configured to be positioned within the interior of the bag and configured to retain and protect the sensing device, wherein the tray comprises an opening configured to allow a button of the sensing device to be engaged via a portion of the bag, thereby allowing the sensing device to be transitioned from a non-operational state to an operational state when retained by the tray and positioned within the interior of the bag. Such operational state can be one in which the sensing device can be wirelessly paired with a separate electronic device, for example, to enable wireless data transmission.

The invention relates to genetic breeding, in particular to a method for cultivating kelp triploid by inducing a monosexual dihaploid (DH) gametophyte. The method specifically comprises the following steps: establishing a kelp DH sporophyte culture system, inducing DH sporophytes to generate unisexual DH gametophytes (2n) through spore-free propagation, and then hybridizing the unisexual DH gametophytes (2n) with haploid (n) gametophytes with opposite sex to obtain kelp triploid (3n) sporophytes. And determining the relative content of DNA in the nucleus of the hybrid sporophyte by using a flow cytometry, thereby identifying the chromosome ploidy of the hybrid sporophyte as 3n. The method can be used for cultivating new varieties of high-yield, high-quality, stress-resistant and sterile kelp, and has important significance for developing genetic breeding and new variety creation research in kelp and even kelp brown algae.

The invention provides a gene for regulating and controlling thermo-sensitive male sterility characters of plants and application of the gene. Specifically, the thermo-sensitive male sterility gene is a newly identified gene, mutation of a gene coding region of the thermo-sensitive male sterility gene causes generation of thermo-sensitive male sterility characters, and a new material is provided for Chinese cabbage male sterility molecular mechanism research and breeding.

The invention discloses a rice photo-thermo-sensitive male sterility mutant Osgpat6.1, a corresponding gene locus OsGPAT6.1 of the rice photo-thermo-sensitive male sterility mutant Osgpat6.1 and application of a coding protein of the rice photo-thermo-sensitive male sterility mutant The phenotypic gene OsGPAT6.1 for controlling the photo-thermo-sensitive male sterility of the rice is obtained by cloning through phenotypic analysis of a mutant and preliminary positioning of a target gene, an amino acid sequence of the phenotypic gene OsGPAT6.1 is encoded as shown in SEQ ID NO.1 to form a protein, and the gene affects the development process of rice pollen by affecting tapetum degradation. The OsGPAT6.1 gene is subjected to gene editing, or a mutant allele of the OsGPAT6.1 gene is introduced into a conventional rice variety in a hybridization manner, so that a photo-thermo-sensitive male sterile material for a two-line sterile line can be obtained. The gene and the encoding protein thereof can be applied to creation of photo-thermo-sensitive sterile rice and germplasm genetic improvement of the photo-thermo-sensitive sterile rice.

The utility model relates to the technical field of smart home, disclose a multifunctional bedside cabinet, including cabinet and support platform, the inside drawer one and drawer two of cabinet are provided with, the bottom fixed mounting of drawer one has ultraviolet lamp, the inside fixed mounting of drawer two has the refrigerator, the rear part of drawer two is integrated with no condensate water automatic evaporation system, including molecular sieve runner, micro fan, PTC heater and humidity sensor. The multifunctional bedside cabinet, through installing ultraviolet disinfection lamp in the refrigeration drawer, can disinfect the storage article, effectively avoid the pollution problem that medicine, fruit etc. are caused because of the microbial growth in the storage process, especially satisfy the scene demand of medicine etc. to the higher sterility requirement of storage environment, guarantee article use safety, through no condensate water automatic evaporation system, can real -time monitoring the humidity in the refrigeration drawer, promptly adsorb and discharge the water vapor in the drawer, avoid the condition that the article is damp and deteriorated because of the condensate water production in the traditional refrigeration process.

Sterile packaging for an intraocular lens (IOL) delivery system includes a sealed container with an IOL delivery system contained therein, with a cap sealing the container. One or more tamper-evident solutions are provided on the packaging to ensure an indication of non-sterility. This configuration solves certain issues with prior packaging, such as clumsy transfers and bulky packaging which creates storage issues and mitigates sterility barrier risk associated with pouch or blister packaging or other non-rigid package types.

Some aspects are generally related to gripper systems for manipulating holders and / or containers disposed therein for use with radionuclide generators. In some embodiments, the gripper system further facilitates the insertion and / or removal of a stopper from the container, which may maintain a sterility of an interior of the container before and / or after exposure of the container to radionuclides from the radionuclide generator.

This invention provides four InDel molecular markers closely linked to the ecotype sterility trait in Chinese cabbage, as follows: Molecular Markers Brvms5 -5216342, nucleotide sequence as shown in SEQ ID NO.1; molecular marker Brvms5 -6164784, nucleotide sequence as shown in SEQ ID NO.2; molecular marker Brvms5 -6315378, nucleotide sequence as shown in SEQ ID NO.3; molecular marker Brvms5 -6648913, nucleotide sequence as shown in SEQ ID NO.4. This invention focuses on the genetic locus mapping of the newly discovered ecotype sterile material LY25 in Chinese cabbage, revealing that its sterility characteristic is influenced by a single-gene recessive genetic locus. Brvms5 Compared to the existing inheritance patterns of temperature-sensitive male sterility and multiple alleles in Chinese cabbage, this method has simpler genetic rules and is more convenient to use. When producing hybrids, it is only necessary to... Brvms5 The site can be imported into the parent material.

The utility model discloses a filter assembly and filter system, wherein the filter assembly includes liquid module, liquid outlet module, main fluid filter and the main air filter of the upstream of main fluid filter, and is sealedly connected first waste container on liquid outlet module, sets up second waste container at the first downstream end of main air filter, and the inside of first waste container and second waste container is isolated with outside, has improved the internal sealing of filter assembly, first waste container and second waste container can respectively undertake wetting liquid and test gas, avoid wetting liquid and test gas direct emission to the environment, can guarantee the sealing of whole flow path inside and the sterility requirement of use environment, and wetting liquid and test gas flow into the corresponding container in waste module, also realize the end point indication of wetting and integrity tester.