237 results about "Sterility" patented technology

The invention discloses a male sterility gene ZmUCE4 and application thereof in creating a male sterility line of corn, and belongs to the field of plant biotechnology breeding. According to the invention, site-directed mutagenesis of the ZmUCE4 gene in wild type corn is carried out through a CRISPR / Cas9 gene editing technology, pollen development abnormality and abortion can be caused, complete male sterility is caused, and it is proved that the ZmUCE4 gene has a regulation and control function on corn male reproductive development. Through offspring screening, a sterile line without transgenic components can be obtained, a stable maize male sterile line is created, and the method has important significance on maize male fertility control and hybrid seed production. The invention also designs a functional molecular marker aiming at the obtained uce4 male sterility mutant gene, and has important application value in maize male sterility line cultivation, sterile hybrid seed production and molecular marker-assisted selection.

The invention provides an integrated storage and sterilization device for multi-specification cell culture apparatuses, and belongs to the technical field of crossing of biomedical engineering and laboratory intelligent equipment. The device comprises a multi-specification cell culture appliance information acquisition module, a sterilization parameter intelligent calculation module, a sterilization process execution and real-time monitoring module, a sterilization effect intelligent judgment module and a multi-specification cell culture appliance storage adaptation module. According to the invention, through integration of an integrated architecture and an algorithm, sterile treatment, intelligent management and full-process tracing of multi-specification cell culture apparatuses are finally realized, high requirements of biomedical experiments on sterility, adaptability and controllability of the apparatuses are met, meanwhile, the operation efficiency of a laboratory is improved, and personal errors and pollution risks are reduced.

The invention discloses a freeze-drying automatic conveying system, which relates to the technical field of freeze-drying equipment, and comprises a conveying track which penetrates through a freeze dryer, and the surface of the conveying track is covered with an anti-frosting nano coating. According to the invention, a conveying track covered with an anti-frosting nano coating, an intelligent tray embedded with an RFID chip and a temperature sensor, a transport vehicle carrying visual monitoring, and a bipolar transition cavity module equipped with an electric translation air-tight door and having environment control and sterilization functions are integrated and coordinated by a central controller; full-process automatic and intelligent conveying of materials among freeze-drying pre-treatment, core freeze-drying and post-treatment areas is achieved, then accurate real-time monitoring and full-process tracking of core process parameters are guaranteed, a sterile, low-oxygen and low-humidity environment is rapidly established through an efficient two-stage transition cavity, the pollution risk is reduced, the sterility and stability of products are guaranteed, and the product quality is improved. Therefore, the product quality consistency, the production efficiency and the equipment utilization rate are improved, and the labor cost and the operation risk are reduced.

The invention relates to a male gamete killer gene S19g-A1 of a rice interspecific hybrid sterile S19 seat and application of the male gamete killer gene S19g-A1. The invention provides a rice hybrid sterility related protein S19g-A1. The amino acid sequence of the rice hybrid sterility related protein S19g-A1 is as shown in SEQ ID NO. 2. According to the invention, a key gene S19g-A1 of a rice interspecific hybrid sterile S19 site is researched and identified, function knockout is carried out on African rice S19g-A1, and pollen fertility of a mutant hybrid F1 obtained by carrying out distant hybridization on an obtained African rice s19g-a1 mutant returns to normal. Based on a gene editing technology, the invention develops a method for rapidly creating a hybrid affinity line capable of overcoming sterility of African rice hybrid, effectively eliminates S19 locus mediated interspecific hybrid reproductive disorder, breaks through the bottleneck of rice interspecific hybrid advantage utilization, provides target gene resources for distant hybrid advantage utilization and crop yield improvement, and has a wide application prospect. Good application prospects are realized.

The invention discloses a gene of a rice indica-japonica intersubspecific hybrid sterile locus HS12 and application thereof, and belongs to the field of plant genetic engineering. The locus is positioned at the tail end of a long arm of a chromosome 12, is positioned between molecular markers Sy55 and Sy8, and comprises four fertility related genes iTx1, iTx2, iAd1 and jAd2. By functional knockout or inhibition of expression of the gene iTx1 and / or iTx2, iAd1 and / or jAd2 are / is transferred, a wide-compatibility rice material can be created, meanwhile, a molecular marker for detecting HS12 is further provided and used for filial generation detection and auxiliary breeding, indica-japonica hybrid sterility can be effectively overcome, and the setting rate of offspring is increased.

The invention relates to the technical field of plant breeding, in particular to a method for constructing a dominant male sterile line based on CIF1 gene overexpression. The brand-new dominant male sterility gene CIF1 is found, it is verified that the gene has functions in monocotyledonous and dicotyledonous plants, and dominant male sterility resources are enriched; besides, a method capable of manually controlling the fertility recovery of the dominant sterile line is found, a reproductive breeding line for large-scale propagation is obtained, male sterile filial generations can be obtained by hybridizing the breeding line with normal plants, and a new application way is provided for large-scale application of dominant sterility.

Injectable compositions and methods of preparation, as well as therapeutic uses, of induced pluripotent stem cell (iPSC)-derived notochordal cell (iNC)-loaded microgels are provided. Microfluidic on-chip platform can be utilized to prepare microgels (or microgel particles / spheres) formed from block copolymers that exhibit reverse thermal gelation, so as to encapsulate iNCs. Also provided are preconditioned iNC-loaded microgels and iNCs in bulk hydrogel. Cell purity, identity, viability, sterility, and the stability of microencapsulated iNCs have been evaluated. Safety and efficacy of the compositions as therapeutic candidates has been tested via intradiscal injection in animal models of intervertebral disc (IVD) degeneration and discogenic low back pain. Biobehavioral testing, MRI, and immunohistochemical analyses were utilized to evaluate the regenerative potential and reproducibility of the compositions as therapeutic candidate. Single cell RNA sequencing of the treated IVDs may also reveal mechanism of action of the compositions.

The invention discloses a KASP molecular marker for identifying a Chinese cabbage nuclear male sterility gene and application of the KASP molecular marker. The invention belongs to the field of molecular biology, and particularly relates to a KASP molecular marker for identifying a Chinese cabbage nuclear male sterility gene and application of the KASP molecular marker. The method for identifying or assisting in identifying the Chinese cabbage fertility comprises the steps that the genotype of an SNP site in a Chinese cabbage genome to be detected is detected, the Chinese cabbage fertility is identified or assisting in identifying according to the genotype, the SNP site is an SNP site on a Chinese cabbage A07 chromosome, the nucleotide type of the SNP site is C or T, and the SNP site is the 1935th nucleotide of SEQ ID No: 4 in a sequence table. The SNP locus related marker can be used for Chinese cabbage male sterility molecular marker assisted breeding, and a new gene resource is provided for Chinese cabbage heterosis utilization.

The invention belongs to the field of plant biotechnology breeding, and relates to an application of a ZmGalAT1 gene and an encoding protein thereof in corn fertility control. According to the invention, a corn male sterility mutant galat1 is obtained by EMS mutagenesis, and a gene ZmGalAT1 for controlling corn male flower development is identified through map-based cloning. The gene is subjected to site-directed mutagenesis in wild type corn through a CRISPR / Cas9 gene editing technology, three galat1 allelic corn male sterility mutants are created, corn male flowers of the mutants are completely aborted, sterile mutants without transgenic ingredients can be obtained through offspring screening, and the mutants are further applied to corn sterile breeding and seed production. The invention also designs functional molecular markers for three allelic male sterile mutants of GalAT1, and has important application value in maize male sterile line cultivation, sterile hybrid seed production and molecular marker-assisted selection.

This invention belongs to the field of molecular genetics and discloses a method and application for controlling fish fertility using in vivo gene editing technology. The applicant, for the first time, cloned a carp-specific U6 promoter and used this promoter to construct the U6 gene. MOVIE gRNA transgenic vectors and water The Cas9 vector allows for the establishment of an in vivo editing system. This strategy enables the heritability of sterility, a reproductive control trait, through hybridization of fertile parents to produce sterile offspring. The operation is highly efficient, simple, and environmentally friendly. Furthermore, since high-copy-rate short, scattered repetitive sequences exist in all fish species, this strategy has broad applicability across species.

According to the analyte detection device installation unit with the sterile barrier, before installation is carried out, the subcutaneous penetrating part of an auxiliary needle is contained in a needle body containing cavity of an outer cover body, a main sterile barrier is arranged at the contact position of the outer cover body and a first through hole, and therefore after the subcutaneous penetrating part is subjected to directional irradiation sterilization, the auxiliary needle can penetrate through the needle body containing cavity of the outer cover body; the subcutaneous piercing part is located in a closed sterile environment, germs existing in the unsterilized part in the installation unit cannot enter the sterile environment, the subcutaneous piercing part cannot carry germs again after directional sterilization, health of a user cannot be affected, and use safety of the user is guaranteed.

The invention relates to the technical field of forest cultivation, and discloses a carya illinoensis fruit forest variety configuration method which comprises the following steps: configuring a main cultivation variety and a pollination variety in a fruit forest; preparing a pollination synergistic composition; the composition is sprayed at the initial stage of stigma exposure of female flowers of a main cultivated variety. The composition comprises a phenolic acid grafted carboxymethyl chitosan derivative, low methoxyl pectin, a calcium ion cross-linking agent, sugar alcohol chelated boron and spermidine. According to the method, an interfacial film with antioxidant activity is formed on the surface of the stigma by using the grafting modified matrix, so that the stigma browning is effectively inhibited, and the pollination period is prolonged; by constructing a calcium cross-linked low-methoxyl pectin interpenetrating network, an interfacial film is endowed with an intelligent degradation characteristic of enzyme-sensitive response to pollen, and protection at ordinary times and immediate dissolution after touching flowers are realized. According to the method, pollen is captured through static electricity, nutritional factors are released in a targeted mode, the pollination and fertilization efficiency is remarkably improved, the sterility risk in the flowering phase is relieved, and the yield and quality of carya illinoensis are improved.

The invention relates to genetic breeding, in particular to a method for cultivating kelp triploid by inducing a monosexual dihaploid (DH) gametophyte. The method specifically comprises the following steps: establishing a kelp DH sporophyte culture system, inducing DH sporophytes to generate unisexual DH gametophytes (2n) through spore-free propagation, and then hybridizing the unisexual DH gametophytes (2n) with haploid (n) gametophytes with opposite sex to obtain kelp triploid (3n) sporophytes. And determining the relative content of DNA in the nucleus of the hybrid sporophyte by using a flow cytometry, thereby identifying the chromosome ploidy of the hybrid sporophyte as 3n. The method can be used for cultivating new varieties of high-yield, high-quality, stress-resistant and sterile kelp, and has important significance for developing genetic breeding and new variety creation research in kelp and even kelp brown algae.

The invention provides a gene for regulating and controlling thermo-sensitive male sterility characters of plants and application of the gene. Specifically, the thermo-sensitive male sterility gene is a newly identified gene, mutation of a gene coding region of the thermo-sensitive male sterility gene causes generation of thermo-sensitive male sterility characters, and a new material is provided for Chinese cabbage male sterility molecular mechanism research and breeding.

The invention discloses a rice photo-thermo-sensitive male sterility mutant Osgpat6.1, a corresponding gene locus OsGPAT6.1 of the rice photo-thermo-sensitive male sterility mutant Osgpat6.1 and application of a coding protein of the rice photo-thermo-sensitive male sterility mutant The phenotypic gene OsGPAT6.1 for controlling the photo-thermo-sensitive male sterility of the rice is obtained by cloning through phenotypic analysis of a mutant and preliminary positioning of a target gene, an amino acid sequence of the phenotypic gene OsGPAT6.1 is encoded as shown in SEQ ID NO.1 to form a protein, and the gene affects the development process of rice pollen by affecting tapetum degradation. The OsGPAT6.1 gene is subjected to gene editing, or a mutant allele of the OsGPAT6.1 gene is introduced into a conventional rice variety in a hybridization manner, so that a photo-thermo-sensitive male sterile material for a two-line sterile line can be obtained. The gene and the encoding protein thereof can be applied to creation of photo-thermo-sensitive sterile rice and germplasm genetic improvement of the photo-thermo-sensitive sterile rice.

The utility model relates to the technical field of smart home, disclose a multifunctional bedside cabinet, including cabinet and support platform, the inside drawer one and drawer two of cabinet are provided with, the bottom fixed mounting of drawer one has ultraviolet lamp, the inside fixed mounting of drawer two has the refrigerator, the rear part of drawer two is integrated with no condensate water automatic evaporation system, including molecular sieve runner, micro fan, PTC heater and humidity sensor. The multifunctional bedside cabinet, through installing ultraviolet disinfection lamp in the refrigeration drawer, can disinfect the storage article, effectively avoid the pollution problem that medicine, fruit etc. are caused because of the microbial growth in the storage process, especially satisfy the scene demand of medicine etc. to the higher sterility requirement of storage environment, guarantee article use safety, through no condensate water automatic evaporation system, can real -time monitoring the humidity in the refrigeration drawer, promptly adsorb and discharge the water vapor in the drawer, avoid the condition that the article is damp and deteriorated because of the condensate water production in the traditional refrigeration process.

Sterile packaging for an intraocular lens (IOL) delivery system includes a sealed container with an IOL delivery system contained therein, with a cap sealing the container. One or more tamper-evident solutions are provided on the packaging to ensure an indication of non-sterility. This configuration solves certain issues with prior packaging, such as clumsy transfers and bulky packaging which creates storage issues and mitigates sterility barrier risk associated with pouch or blister packaging or other non-rigid package types.

This invention provides four InDel molecular markers closely linked to the ecotype sterility trait in Chinese cabbage, as follows: Molecular Markers Brvms5 -5216342, nucleotide sequence as shown in SEQ ID NO.1; molecular marker Brvms5 -6164784, nucleotide sequence as shown in SEQ ID NO.2; molecular marker Brvms5 -6315378, nucleotide sequence as shown in SEQ ID NO.3; molecular marker Brvms5 -6648913, nucleotide sequence as shown in SEQ ID NO.4. This invention focuses on the genetic locus mapping of the newly discovered ecotype sterile material LY25 in Chinese cabbage, revealing that its sterility characteristic is influenced by a single-gene recessive genetic locus. Brvms5 Compared to the existing inheritance patterns of temperature-sensitive male sterility and multiple alleles in Chinese cabbage, this method has simpler genetic rules and is more convenient to use. When producing hybrids, it is only necessary to... Brvms5 The site can be imported into the parent material.

The utility model discloses a filter assembly and filter system, wherein the filter assembly includes liquid module, liquid outlet module, main fluid filter and the main air filter of the upstream of main fluid filter, and is sealedly connected first waste container on liquid outlet module, sets up second waste container at the first downstream end of main air filter, and the inside of first waste container and second waste container is isolated with outside, has improved the internal sealing of filter assembly, first waste container and second waste container can respectively undertake wetting liquid and test gas, avoid wetting liquid and test gas direct emission to the environment, can guarantee the sealing of whole flow path inside and the sterility requirement of use environment, and wetting liquid and test gas flow into the corresponding container in waste module, also realize the end point indication of wetting and integrity tester.

The invention discloses a corn hdziv4 male sterile line created by using artificial mutation. According to the invention, a corn fertility gene ZmHDZIV4 is edited by using a CRISPR / Cas9 gene editing technology, and then a homozygous male sterile mutant without Cas9 transgenosis is obtained in an F2 generation through hybridization and selfing, so that a stable corn hdziv4 male sterile line is created. The hdziv4 male sterile line and a target material are hybridized and backcrossed, so that the target material can obtain the character of hdziv4 male sterility and gene mutation, and the method has important significance on promoting corn sterile cross breeding and seed production. The invention also designs a functional molecular marker aiming at the obtained hdziv4 male sterile mutant, and the functional molecular marker has important application value in maize male sterile line cultivation, sterile cross breeding, seed production and molecular marker-assisted selection.

The invention relates to the technical field of culture medium filling, and discloses an automatic sterile culture medium filling machine which comprises a machine shell, a workbench, a filling assembly, a blocking structure, a homogenizing structure and a moving structure. The moving structure realizes transverse and longitudinal fixation of the vessel, and the homogenizing structure drives the vessel to do square motion; the problems that existing equipment is uneven in filling and vessels are prone to shifting are solved, the advantages of being high in automation degree and good in sterility guarantee are achieved, the filling efficiency and the culture medium quality are improved, and the labor intensity is reduced.

The invention discloses a high-throughput ByF1 breeding method based on male sterility, and belongs to the technical field of plant hybridization. According to the method, the male sterile line and the restoring line are subjected to mixed sowing and natural pollination, so that rapid creation of million-level F1 combinations can be realized, and about 6000 combinations can be created within one year through only one sterile line, which is equivalent to the sum of annual workloads of 6-15 traditional breeding subjects. In the screened excellent single plants, the combination of 29 dominant parents can be accurately traced through comparison of the whole genome SNP loci; the hybrid wheat variety Jiumai 01 cultivated by the applicant is just one of the 29 dominant parent combinations. The yield advantage of the male parent Shannong 22 of the spring vein 01 planted in the Shahe Sing base of Jinan through testing for many years can reach 11%-31.8%, the feasibility of the method in practice is fully verified, the breeding efficiency of the strong advantage combination of wheat is greatly improved, and technical support is provided for accelerating breeding of hybrid wheat and conventional wheat varieties.

The application belongs to the technical field of crop genetic breeding, and particularly relates to a rice hybrid male sterility site HMS10 Co-segregation molecular marker and application thereof. The present application takes the reverse temperature-sensitive male nuclear sterile line Yanong S as the female parent, crosses and backcrosses with normal fertile material Yanong HB (as the recurrent male parent), and uses a high-generation backcross large population to finely position the site for controlling the hybrid male sterility of rice in a 60 kb physical interval of the 10th chromosome of rice HMS10 , and develops a co-segregation molecular marker IDHMS10. The present application uses the molecular marker IDHMS10 to convert the positive temperature-sensitive male nuclear sterile line Quan211S with excellent comprehensive agronomic traits into a practical rice hybrid male sterile line Quan211HS with fertility not affected by light and temperature, and uses the IDHMS10 for purity identification of the hybrid male sterile line, which has important significance for solving the risk of seed production of two-line hybrid rice and enriching the types of practical sterile resources.

The invention discloses a filtering assembly, a filtering system and an on-line integrity testing method.The filtering assembly comprises a liquid module, a liquid outlet module, a main fluid filter and a main air filter located on the upstream of the main fluid filter, and the liquid outlet module is connected with a first waste discharge container in a sealed mode; the second waste discharge container is arranged at the first downstream end of the main air filter, and the interiors of the first waste discharge container and the second waste discharge container are isolated from the outside, so that the internal sealing performance of the filter assembly is improved; the first waste discharge container and the second waste discharge container can receive wetting liquid and test gas respectively, the wetting liquid and the test gas are prevented from being directly discharged into the environment, the sealing performance in the whole flow path and the sterile requirement of the use environment can be guaranteed, meanwhile, the wetting liquid and the test gas flow into the corresponding containers in the waste discharge module, and the waste discharge efficiency is improved. Endpoint indication of the wettability and integrity tester is also achieved.

This invention discloses a method for constructing a fast-growing, sterile zebrafish model, belonging to the field of fish animal model preparation technology. This invention is the first to discover the role of the CK gene in regulating fish growth rate and fertility: fish with simultaneous knockout of ck1 and ck2 genes exhibit rapid growth and gonadal abortion. Using the method provided by this invention, a fast-growing, sterile zebrafish model was successfully constructed. These zebrafish exhibited gonadal dysplasia and sterility, but also rapid growth. Compared to wild-type zebrafish, the 4-month-old double mutant zebrafish showed significantly increased body length and weight. The disclosed gene function and zebrafish model construction method can be used for breeding fast-growing, sterile fish varieties and studying related mechanisms, which is of great significance for improving the economic benefits of aquaculture and protecting ecological security.

The utility model relates to medical instrument detection field discloses a sterile detection device for medical instrument, including operation platform, the right side fixedly connected with support platform of operation platform, the top of operation platform is provided with detection pipe, the right side of detection pipe is connected with the shunt pipe who sets up, the one end away from detection pipe of shunt pipe is connected with the detection bottle who sets up in support platform top, the outer wall of detection bottle is provided with fixed establishment, the fixed establishment includes positioning assembly and guide assembly, positioning assembly includes two groups of clamping blocks, the left and right sides of support platform all are fixedly connected with fixed block no.

Provided are a particle detection device and a particle detection method that have high particle identification properties, can ensure sterility, and have automation suitability.A particle detection device includes a holding part that holds a suspension containing particles, a first flow channel that is connected to the holding part, an observation window that is connected to the first flow channel, a liquid feeding part that transfers the suspension held in the holding part to the observation window, and a fluorescence detection part that detects fluorescence emitted from the particles contained in the suspension through the observation window.

According to the device for preparing and transferring the sterile liquid medicine, a preparation assembly is used for preparation and sterile treatment of auxiliary materials of the sterile liquid medicine, and the preparation assembly is arranged in a non-sterile environment; the first batching tank is used for preparing main raw materials of the sterile liquid medicine, is arranged in a sterile environment and is connected with the preparation assembly; the second batching tank is movably arranged in a non-sterile environment, the interior of the second batching tank is in a sterile state, and the second batching tank is connected with the first batching tank; the power source device can generate power, so that the sterile liquid medicine auxiliary materials enter the first batching tank after being subjected to configuration and sterile treatment, and carry the sterile liquid medicine main raw materials in the first batching tank to enter the second batching tank; the second mixing tank can be moved to a position close to the filling line and is in sterile butt joint with the filling line. According to the device, continuous supply of materials among different clean environments can be ensured, meanwhile, flexible matching in the sterile liquid medicine preparation process is achieved, and the sterility of the materials can be kept.

The invention relates to a ZmMS50 protein as well as a coding gene and application thereof, and belongs to the technical field of plant gene breeding, the amino acid sequence of the ZmMS50 protein is shown as SEQ ID NO.1, the full-length sequence of the ZmMS50 gene is shown as SEQ ID NO.2, and the sequence of a coding region is shown as SEQ ID NO.3. The male fertility of corn can be controlled by regulating and controlling the expression quantity of the ZmMS50 protein. The invention finds that the ZmMS50 protein is related to the male sterility of the maize cell nucleus, and the ability of inhibiting the expression of the ZmMS50 protein by the male fertile maize enables the male fertile maize to be sterile. The invention provides a solid theoretical basis for establishing the plant male sterility line, and has important significance for promoting the seed production of the corn male sterility line.

The invention discloses a sterile disinfectant preparation and clean area crossing transfer integrated device and method, and relates to the technical field of disinfection equipment. Comprising a configuration mechanism, the configuration mechanism comprises a configuration tank, a discharging opening is formed in the outer wall of the bottom of the configuration tank, an output pipe is fixedly connected to the inner wall of the discharging opening, an outlet diaphragm valve communicating with the output pipe is fixedly connected to the outer wall of the output pipe, and a transfer mechanism is connected to one end of the output pipe. The system integrates the functions of preparation, filtration, transfer, cleaning and sterilization, does not have redundant modules, and effectively reduces the purchase cost of equipment; a standardized 316L stainless steel component and a chuck type interface are adopted, so that the maintenance difficulty is low, and the later maintenance cost is reduced; through multiple protections such as two-stage sterilization filtration, closed transfer, A-stage laminar flow hood receiving and anti-backflow design, it is ensured that the disinfectant is sterile in the whole process of preparation, filtration and cross-regional transfer, the pollution risk is effectively reduced, and the use requirement of a B-stage clean area is met.