45 results about "Sterility" patented technology

This invention belongs to the field of molecular genetics and discloses a method and application for controlling fish fertility using in vivo gene editing technology. The applicant, for the first time, cloned a carp-specific U6 promoter and used this promoter to construct the U6 gene. MOVIE gRNA transgenic vectors and water The Cas9 vector allows for the establishment of an in vivo editing system. This strategy enables the heritability of sterility, a reproductive control trait, through hybridization of fertile parents to produce sterile offspring. The operation is highly efficient, simple, and environmentally friendly. Furthermore, since high-copy-rate short, scattered repetitive sequences exist in all fish species, this strategy has broad applicability across species.

Sterile packaging for an intraocular lens (IOL) delivery system includes a sealed container with an IOL delivery system contained therein, with a cap sealing the container. One or more tamper-evident solutions are provided on the packaging to ensure an indication of non-sterility. This configuration solves certain issues with prior packaging, such as clumsy transfers and bulky packaging which creates storage issues and mitigates sterility barrier risk associated with pouch or blister packaging or other non-rigid package types.

The utility model relates to medical instrument detection field discloses a sterile detection device for medical instrument, including operation platform, the right side fixedly connected with support platform of operation platform, the top of operation platform is provided with detection pipe, the right side of detection pipe is connected with the shunt pipe who sets up, the one end away from detection pipe of shunt pipe is connected with the detection bottle who sets up in support platform top, the outer wall of detection bottle is provided with fixed establishment, the fixed establishment includes positioning assembly and guide assembly, positioning assembly includes two groups of clamping blocks, the left and right sides of support platform all are fixedly connected with fixed block no.

The present application relates to the field of molecular genetic technology, in particular to a KASP molecular marker TMSK23 related to eggplant reverse temperature-sensitive male sterility and application thereof. The molecular marker site is obtained by crossing 05ms as female parent and S132 as male parent, and obtaining F2 separation population. According to the published high-quality eggplant genome information, the whole genome resequencing technology is used to pool and sequence the homozygous dominant fertile plants and homozygous recessive sterile plants in the F2 generation of eggplant, and the SNP difference sites of the fertile gene and sterile gene pool are screened out, and the difference sites are verified by KASP gene typing method and protein three-dimensional structure prediction. The present application solves the problems of large workload in hybrid seed production in the prior art, avoids the problem of a large number of measurement and matching in the transgenic process, improves the breeding efficiency and has other advantages.

The upper surgical field for cesarean section intervention with neonatal transfer aims to eliminate the current limitations of the "humanized cesarean" by creating a circuit for direct transfer of the newborn to its mother within the surgical field and without altering asepsis. This internal communication is achieved by means of a sterile main drape with a fenestration in its caudal portion that continues perpendicularly with a transfer module located over the maternal thorax, a non-sterile area, with an integrated closure system. Additionally, the main drape has a secondary drape designed to seal the fenestration. This surgical field allows for maintaining sterility during neonatal transfer, optimizes delayed clamping of the umbilical cord, ensures the preservation of heat and continuous visualization of the newborn, achieves immediate skin-to-skin contact, and increases maternal participation in welcoming the newborn.

This invention belongs to the pharmaceutical field, specifically relating to a sodium nitroprusside injection and its preparation method. The sodium nitroprusside injection comprises the following raw materials in parts by weight: 1.5–3.5 parts sodium nitroprusside and 100 parts water for injection. This invention solves the problem of poor stability of sodium nitroprusside aqueous solutions by optimizing the formulation and preparation process without introducing other excipients. The sodium nitroprusside injection prepared by this invention is a sterile injectable preparation manufactured using modern aseptic filling technology combined with non-terminal sterilization. It not only has the advantages of direct and rapid action and high sterility assurance, but also has few adverse reactions and can be transported and stored at room temperature, thus improving the stability and safety of the product. This invention also provides its preparation method, which is simple, safe to use, and has stable quality.

This invention discloses a low-temperature, low-humidity sterilization device for biodegradable materials used in cardiac occlusion, relating to the field of sterilization technology. The oscillating irradiation mechanism includes a motor and two fixing bars fixedly connected to the top of the sterilization chamber. A cable tray is fixedly connected to the top of the motor, and a rotating disk is fixedly connected to the output end of the motor. A swing shaft is rotatably connected between the inner walls of the two fixing bars. By setting up the oscillating irradiation mechanism, the ultraviolet sterilization lamp at the bottom of the mounting frame oscillates in a fan shape, and the illumination angle is dynamically adjusted in real time with the movement of the mechanism. This allows the ultraviolet light to fully cover all areas inside the sterilization chamber, completely eliminating the sterilization dead zones caused by the fixed irradiation angle and range of traditional fixed-point sterilization lamps. This ensures that all parts of the biodegradable cardiac occlusion material are effectively irradiated, killing all hidden microorganisms and meeting the sterility requirements for medical implants.

The present application relates to the field of agricultural technology, in particular to a plant temperature-sensitive male sterility gene mutant tms17 and application thereof.The nucleotide sequence of the plant temperature-sensitive male sterility gene mutant tms17 comprises the nucleotide sequence shown in SEQ ID NO.1 in the sequence listing.The present application screens a new temperature-sensitive male sterile line tms17 by chemical mutagen ethyl methyl sulfone (EMS) mutagenesis of japonica rice variety ZH11, which is completely sterile at high temperature and fertility is restored at low temperature, and the mechanism of temperature-sensitive fertility restoration is disclosed, which provides a new germplasm resource with application potential for rice two-line hybrid breeding.

The invention is a dynamic packaging system for wearable biosensors including components for securing the biosensor in place, for assisting insertion of the biosensors into the skin of a user in a fashion which guides the user to an appropriate amount of force, for sensing environmental conditions, for communicating with the biosensor and another external device running a mobile app. A receptacle holding the biosensor is the location for various electrical components enabling these functions. In separate embodiments, a cap or a peelable film is provided for a tight seal to preserve sterility inside the packaging system prior to insertion of the biosensor.

The application relates to the technical field of capsule weighing, and discloses an automatic weighing device for capsule production, which comprises a bottom plate, two parallel installation plates fixedly connected to the bottom plate, a feeding box for placing capsules, the feeding box being located between the two installation plates and fixedly connected with the installation plates, a movement assembly arranged in the feeding box, a plurality of communication pipelines communicated with the bottom surface of the feeding box and matched with the capsules in inner diameter, a rotating bin fixedly installed between the two installation plates, the rotating bin being communicated with the communication pipelines at the top, a distribution assembly rotatably arranged in the rotating bin, a plurality of discharge ports arranged at the bottom of the rotating bin, and a discharge assembly located below the rotating bin and communicated with the discharge ports. The application realizes precise static weighing of single capsules and continuous automatic production, significantly improves the weighing accuracy and production efficiency, and meets the production requirements of the pharmaceutical industry in terms of large scale and sterility.

A female genitalia protective sheath apparatus is disclosed for providing a flexible, anatomically conforming, and hygienic barrier over external female genitalia. The apparatus includes a multilayer structure comprising a stimulus pad positioned between first and second protective layers, each supported by adhesive frames and removable covers to maintain sterility prior to use. The apparatus may further include an almond-shaped contoured frame, a semi-covered access region, a tear-away perimeter for controlled removal, and an optional magnetic coupling system for repeatable attachment. A handle facilitates positioning and removal while minimizing contact with the barrier surface. During use, the apparatus adheres securely to the skin, conforming to body movement while preventing fluid exchange and reducing the risk of pathogen transmission. The device provides an ergonomic, disposable, and female-oriented protective solution suitable for personal and clinical applications.

The application provides a method for improving the hybridity of inter-subspecies combination of rice and a breeding method, and belongs to the technical field of genetic engineering breeding. The application provides a method for improving the hybridity of inter-subspecies combination of rice, which comprises the following steps: knocking out at least one site in the sterility site of inter-subspecies hybrid of D. subsp. non-cultivated rice S1 - OsORF6 and knocking out at least one site in the wide compatibility gene of rice S5 - ORF5 . The application finds that the gene knockout of each at least one site in the two genes of the sterility site of inter-subspecies hybrid of D. subsp. non-cultivated rice S1 - OsORF6 and the wide compatibility gene of rice S5 - ORF5 can improve the hybridity of inter-subspecies combination, thereby improving the hybrid seed setting rate of inter-subspecies, and has no influence on other traits.

A system and method for collecting and storing urine without invasive medical procedures while maintaining sterility for medical testing. The system includes an absorbent component for collecting urine. The system may include an extraction system. The system may include a tester. For example, the tester may include an indicator (e.g., a test strip and / or a rapid diagnostic test) and / or a sensor and / or an automated tester.

The injection device (1) includes: a housing (2) containing a reservoir (11) for storing a predetermined volume of medical product; a patch (22) disposed on the housing (2) for allowing the injection device (1) to be attached to an injection site; and a fluid chamber (5) configured to fluidly connect the reservoir (11) to a catheter (32), the fluid chamber (5) being axially movable together with the catheter (32) between an initial position and an activated position distal to the initial position, in which the catheter (32) is deployed outside the housing (2) to penetrate into the injection site. The device further includes: a sealing member (4) removably attached to the fluid chamber (5) and arranged around the conduit (32) to maintain sterility; a support member (7) movable between a blocking position and a release position, wherein in the blocking position the support member (7) blocks the movement of the fluid chamber (5) so that the fluid chamber (5) cannot reach the activation position, and in the release position the support member (7) no longer prevents the fluid chamber (5) from reaching the activation position; and a remover (6) configured to first remove the sealing member (4) from the fluid chamber (5) and then displace the support member (7) from the blocking position to the release position.

This medical pump provides a system for isolating medical fluids being injected into a patient's body while maintaining sterility. [Solution] A pump for treating a patient is disclosed, comprising a spring-loaded plunger 116 biased in a direction acting relative to a peristaltic pump tube, a camshaft 190 configured to actuate the spring-loaded plunger, an actuatable lever 104, a shaft coupled to the lever and having a central axis along the length of the shaft and coupled to the lever so as to rotate about the central axis in accordance with the operation of the lever, and a lift cam pivotally coupled to the shaft and pivoting about a lift cam axis, the lift cam axis of the lift cam being parallel to the central axis of the shaft, and as the shaft rotates in accordance with the operation of the lever to the open position, the lift cam engages with the spring-loaded plunger and lifts the spring-loaded plunger away from the camshaft.

This invention provides GhFRC1 The application of genes in regulating plant fertility belongs to the field of plant genetic engineering technology. This invention is the first to clone genes from cotton. GhFRC1 The gene was identified and verified to be heterologously expressed in three different genera and families of crops: Arabidopsis thaliana, tomato, and rice, and to play a role in fertility regulation. This breakthrough overcomes the limitation of existing fertility genes that can only regulate the fertility of a single crop, and realizes "broad-spectrum regulation of fertility of multiple crops by a single gene", filling the gap in the application of distant heterologous expression of cotton fertility genes. GhFRC1 After heterologous expression of the gene, it can stably lead to inactive pollen and abnormal fruit set in the recipient crop, and can also cause a phenotype of complete pollen sac non-development. Moreover, the sterility trait can be restored to fertility through normal pollen pollination. This solves the problem of the narrow applicability of existing fertility genes and the great limitation of their breeding applications, and provides new gene resources and technical solutions for fertility regulation of multiple crops and hybridization breeding.

The application discloses a pepper cell nucleus male sterility molecular marker and application thereof. The pepper cell nucleus male sterility molecular marker disclosed by the application is a DNA fragment corresponding to sequence 3 in a sequence list in a pepper genome, and the pepper genome contains or does not contain the DNA fragment of sequence 3 in the sequence list. Experiments prove that in a pepper 20Q5066AB population of 554 strains, there are 281 fertile strains, all of which are fertile in field fertility identification and all contain the DNA fragment shown in sequence 3 in the sequence list; there are 273 sterile strains, all of which are male sterile in field fertility identification and all do not contain the DNA fragment shown in sequence 3 in the sequence list. It is shown that the pepper male sterility molecular marker of the application can be used for identifying pepper male sterility, has very strong applicability, and can be used for fertility rapid identification in practical breeding application.

This invention discloses a recessive nuclear male sterility gene. BoaGMS1 Application in regulating male development in Chinese kale. This invention screens recessive nuclear male sterility mutants from Chinese kale breeding and isolates the Chinese kale male fertility gene using forward genetics. BoaGMS1 Further experiments confirmed that BoaGMS1 A gene mutation resulted in recessive nuclear male sterility in Chinese kale. Meanwhile, targeting... BoaGMS1 Cosegregating molecular markers developed for male-sterile lines can be used for identifying fertility alleles in plants, screening target plants in marker-assisted breeding, and determining seed purity. Furthermore, they can be used in the development of kale. BoaGMS1 Male-sterile lines can be applied to the breeding and production of new hybrid varieties of Chinese kale. Therefore, the male fertility gene for Chinese kale provided by this invention... BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS It plays an important role in the utilization of heterosis and hybrid seed production of Chinese kale and even Brassica oleracea vegetables.

An object of the present invention is to provide a fish individual which, as shown in FIG. 2, makes it possible, in producing a germ-cell deficient individual, to identify a genotype of a sterilization protein by appearance without collection of a portion of a fish body. This object is achieved by a fish individual deficient in at least a part of a function of the sterilization protein, the fish individual having, in at least one of sterilization gene alleles encoding the sterilization protein which indicates sterility by at least the part of the function thereof being deficient, a polynucleotide sequence encoding the fluorescent protein to be expressible.

The application discloses a method for constructing a fluorescently labeled piwil1 gene knockout sterile zebrafish line, which utilizes a CRISPR / Cas9 system to construct a piwil1 gene knock-in vector carrying P2A-EGFP or P2A-mCherry, respectively, to obtain two hybrid strains through microinjection, and then to screen a homozygote expressing EGFP and mCherry simultaneously by mating the two strains. The homozygote shows male sterility and can be used for reproductive development research and ecological prevention and control of transgenic fish. The application realizes visual labeling of the function of the piwil1 gene and accurate screening of the sterile phenotype, and is simple and efficient to operate.

This invention belongs to the field of feed processing technology, and particularly relates to a method for grading and sterilizing SPF animal feed and its application in preserving the heat-sensitive nutrients in SPF animal feed. A method for grading and sterilizing SPF animal feed includes the following steps: classifying the SPF animal feed raw materials to be sterilized into two categories based on their heat sensitivity; subjecting the heat-resistant base raw materials to high-temperature and high-pressure steam sterilization; subjecting the vitamin premix to encapsulation and / or carrier adsorption treatment; subjecting the enzyme preparation to low-temperature steam sterilization and spraying with an enzyme stabilizer solution; adding the probiotic preparation to the heat-resistant base raw materials; and mixing the treated raw materials under aseptic conditions to obtain the graded and sterilized SPF animal feed. This invention implements a process of grading, preservation, and then mixing, which can increase the vitamin A retention rate to over 80% and the phytase activity retention rate to over 75%, while ensuring that the sterility meets the requirements for SPF grade.

The application discloses a sterile examination device, which comprises a double-layer tank body, a microporous filter film, a sterile absorption pad and an exhaust filtering assembly, wherein the double-layer tank body comprises an inner cavity and an outer cavity outside the inner cavity, and a lower chamber of the inner cavity is communicated with the outer cavity through a small hole; the microporous filter film is fixed in the middle part of the inner cavity and divides the inner cavity into an upper chamber and the lower chamber; the sterile absorption pad is located below the microporous filter film, and a neutralizing agent is prearranged in the sterile absorption pad; the exhaust filtering assembly is communicated with the upper chamber of the inner cavity, and a hydrophobic membrane is arranged in the exhaust filtering assembly. The sterile examination device and the detection method thereof can first realize bidirectional flushing and neutralization of the absorption pad, can efficiently remove bacteriostatic substances on both sides of the filter film, and can avoid false negative; secondly, the device can realize online integrity test, can prevent false positive caused by leakage of the device, and can realize full-closed operation, so that the risk of external source pollution is reduced; and meanwhile, the device is suitable for sterile examination of difficult-to-filter samples containing preservatives, antibiotics and the like.

The present application relates to the field of molecular crop breeding, and relates to a construct for mediating maize male sterility and application thereof, the construct comprising four functional element expression cassettes, namely a red fluorescent gene DsRed expression cassette, a male fertility restoration gene MS45 expression cassette, a tassel pollen lethal gene ZmAmylase expression cassette and a glufosinate-ammonium herbicide tolerance gene PAT expression cassette. The seed of the offspring plant with the specific construct obtained by the present application can be directly observed by naked eyes under the condition of not using other equipment, and the seed has glufosinate-ammonium herbicide resistance, so that the screening mode is greatly simplified, and a large amount of labor cost is saved; meanwhile, the herbicide resistance is generated, which has an important role for field screening or killing weeds.

This invention discloses a method for effectively reducing the cooked flavor of aquatic product compound sauces after heat sterilization, belonging to the field of food processing technology. The method includes: heating the formula with vegetable oil to a suitable temperature, adding capsanthin and one or more fat-soluble antioxidants selected from vitamin E, rosemary extract, and ascorbyl palmitate for pretreatment; adding modified porous starch-based composite material and yeast extract to reconstruct the microenvironment when the sauce cools to below a specified temperature; subsequently performing gradient vacuum degassing; and finally employing a mild, segmented heat sterilization process that uses low-temperature maintenance to activate endogenous enzymes to form a structural barrier, high-temperature instantaneous sterilization to achieve commercial sterility, and rapid cooling to terminate the thermal reaction. This invention inhibits the generation of off-flavor substances during heat sterilization at the reaction mechanism level, while promoting the production of pleasant flavors, significantly reducing the cooked flavor of the product, and improving its umami flavor and textural quality. It is applicable to the production of various aquatic product compound sauces.

A material transfer system (10) made up of first and second mated identical ports (20, 30) in which each port (20, 30) can function as either the receiving and dispensing port for materials thus eliminating the need for separate alpha and beta ports in which the system employs a geared and cammed operating mechanism (160) for precise movement and locking, ensuring pressure stability and sterility throughout material transfer operations.

This invention discloses a male infertility gene. ZmC3H3 This invention, and its application in creating male-sterile maize lines, belongs to the field of plant biotechnology breeding. By using CRISPR / Cas9 gene editing technology to perform site-directed mutations in this gene in wild-type maize, abnormal pollen development and complete male abortion can be caused, demonstrating… ZmC3H3 Genes play a regulatory role in male reproductive development in maize. Through offspring screening, stable male-sterile maize lines without transgenic components can be obtained, which is of great significance for controlling male fertility and producing hybrid seeds in maize. This invention also addresses the obtained... c3h3 Functional molecular markers were designed for the male sterility mutant gene, which have important application value in the breeding of male sterile maize lines, sterile hybridization seed production, and molecular marker-assisted selection.

The present application relates to the application of SPEM3 protein or TEX50 protein in the microstructure display of the post-loop of sperm, and the preparation of a diagnostic agent for sperm deformity, and further relates to a knockout Spem3 or Tex50 mammalian male sterility model, and a sperm post-loop visualized animal model with a displayable label on the SPEM3 protein molecule or TEX50 protein molecule, and the application thereof. The present application discloses the post-loop localization of SPEM3 and TEX50 in mouse and human sperm, and the combination Spem3 – / – and Tex50 – / – of SPEM3 and TEX50 in the diagnosis and treatment of sperm deformity.

The present invention belongs to the field of plant genetic engineering. Provided is a gene ZmKO1 for regulating flowering and pollen shedding of a maize tassel. Mutating the gene ZmKO1 can lead to a genic male sterility phenomenon. The breeding of a maize genic male sterile line can be achieved on the basis of the gene ZmKO1 site using the CRISPR-Cas9 technology or mutant gene backcross breeding technology. In the silking stage of the sterile line, spraying exogenous gibberellin on the tassel can restore fertility in male flowers of the sterile line, enabling normal self-pollination and reproduction, thereby achieving an economical and efficient maintenance of the sterile line as a maintainer line. The genic male sterile line created on the basis of the ZmKO1 gene site has the characteristic of one line for two uses, and can be used for maize male sterile hybrid seed production. Further provided is a technical system for achieving maize two-line sterile hybrid seed production using ZmKO1 gene mutations.

The application discloses a gene KNU for controlling navel orange male sterility and application thereof. The nucleotide sequence of the gene KNU for controlling navel orange male sterility is shown as SEQ ID NO:1. The amino acid sequence of a protein KNU for controlling navel orange male sterility encoded by the gene KNU for controlling navel orange male sterility is shown as SEQ ID NO:2. The application identifies a key gene KNU for controlling male sterility and seedlessness from navel orange and its back-mutants. The gene KNU is a gene specifically expressed in anther somatic cells, and through a Cas9 editing mode citrus material, precise breeding of male sterility and seedless citrus can be realized. The application first provides a target gene KNU for controlling citrus male sterility and seedlessness.