539 results about "Sterility" patented technology

The invention belongs to the technical field of crop genetic breeding, and particularly relates to a rice hybrid male sterility site HMS10 co-segregation molecular marker and application thereof. The method comprises the following steps: hybridizing and backcrossing an anti-thermo-sensitive type genic male sterile line Yannong S serving as a female parent with a normal fertile material Yannong HB (serving as a recurrent male parent), finely positioning to a site HMS10 for controlling hybrid male sterility of rice in a physical interval of-60kb of a chromosome 10 of the rice by utilizing a high-generation backcross large population, and developing a co-separation molecular marker IDHMS10. The molecular marker IDHMS10 is utilized, through a molecular breeding strategy, a positive temperature-sensitive male genic male sterile line Quan 211S with excellent comprehensive agronomic traits is transformed into a practical rice hybrid male sterile line Quan 211HS with fertility not affected by light and temperature, and the IDHMS10 is used for purity identification of the hybrid male sterile line, so that the molecular marker IDHMS10 has great significance in solving the seed production risk of two-line hybrid rice and improving the yield of the hybrid male sterile line. And the method has important significance in enriching practical sterile resource types and the like.

A filtration module is provided, the module including at least one filtration packet containing filtration media or one or more membranes, such as a stack of membranes, the at least one filtration packet having one or more fluid ports, the one or more fluid ports being surrounded by a primary seal and a secondary seal spaced from the primary seal. The secondary seals are designed to maintain sterility of the assembly during shipping, handling and / or installation. A removable film may cover one or more fluid ports to maintain sterility prior to use.

A closure system for a medication container, the interior of which is accessible via a container opening designed in the manner of a bottle mouth, comprising a bump cap with an annular lid having a central opening, on the outer circumference of which is arranged a number of latching elements which can be brought into engagement with an outer bead mounted circumferentially on the container opening, and a one-piece closure plug with a closure body which completely fills the central opening of the annular lid and can be brought into latching engagement therewith, is intended to enable continuous compliance with even high sterility requirements during handling in a simple and reliable manner. For this purpose, the closure body is designed as a duckbill valve.

The invention discloses a rice OsMATE7 gene and application thereof, and belongs to the technical field of molecular biology. According to the application disclosed by the invention, knockout plants KO1 and KO2 of the OsMATE7 gene are constructed, then phenotypes of the knockout plants are identified and analyzed, and the knockout plants of the OsMATE7 gene are found to be almost not firm. Then analyzing the fertility of floral organs and pollen grains of the knockout plant to find that the pollen fertility of the knockout plant of the OsMATE7 gene is not influenced, but the length of a pollen tube is reduced, so that fertilization cannot be normally completed. Therefore, the OsMATE7 gene can regulate and control the male sterility of the rice. According to the research, the rice OsMATE7 gene is analyzed in rice for the first time, and a transgenic experiment proves that the gene can regulate and control rice fruiting by influencing the length of a rice pollen tube, so that a new male sterility gene resource is provided for rice breeding.

The invention discloses a male sterility gene ZmUCE4 and application thereof in creating a male sterility line of corn, and belongs to the field of plant biotechnology breeding. According to the invention, site-directed mutagenesis of the ZmUCE4 gene in wild type corn is carried out through a CRISPR / Cas9 gene editing technology, pollen development abnormality and abortion can be caused, complete male sterility is caused, and it is proved that the ZmUCE4 gene has a regulation and control function on corn male reproductive development. Through offspring screening, a sterile line without transgenic components can be obtained, a stable maize male sterile line is created, and the method has important significance on maize male fertility control and hybrid seed production. The invention also designs a functional molecular marker aiming at the obtained uce4 male sterility mutant gene, and has important application value in maize male sterility line cultivation, sterile hybrid seed production and molecular marker-assisted selection.

A sterilization validation system and method are disclosed that ensure sterilization of packaging materials that contain certain sterilized items that have been sterilized in a cleanroom environment. In accordance with the disclosed subject matter, garments (or other items or materials) that are sterilized inside autoclave or breather bags may be secured within tote liners that ensure sterility of the exterior of the breather bags containing the garments. Not only is it possible to ensure a recipient that the garment is sterile, but it is also possible to verify that the outside of the breather bag itself is also sterile, since the breather bag will remain sterilized inside the tote liner during shipping.

The invention belongs to the technical field of biology, and particularly relates to a Yu-grain rpl16 gene and application thereof, in particular to application of the rpl16 gene in cultivation of a rice sterile line. Overexpression of the Yu-grain rpl16 gene in rice can cause rice pollen abortion, so that rice male sterility is realized, the breeding period is shortened, the breeding efficiency is improved, and theoretical guidance is provided for culturing a new male sterility line.

A convenience kit being formed by a pair of associated sub-kits which provide capability for bench-top medicine prescription compounding without need for clean room surroundings or such protective equipment as laminar flow hoods. The sub-kits include an exterior sub-kit which provides for access, volumetric measurement and compounding of medicine derived from vials and an enclosing sub-kit housed within a plastic bag which provides a sterile environment for filling a medical treatment vessel for use. The two sub-kits are joined together and communicate by bag entry technology to provide a system which not only protects and, in some cases, provides product sterility, but also assures full containment of all fluid being used in a medical preparation.

The invention discloses a gene ZmKO1 for regulating flowering and pollination of corn male flower spikelet and application of the gene ZmKO1 in corn male sterility hybrid seed production, and belongs to the field of plant genetic engineering. The invention provides a gene ZmKO1 for regulating and controlling flowering and pollination of maize male flower spikelet, and the phenomenon of nuclear male sterility can be caused by mutation of the gene ZmKO1. It is also clear that on the basis of the ZmKO1 gene locus, a CRISPR-Cas9 technology or a mutant gene backcross transformation technology is utilized, and cultivation of the maize nuclear male sterile line can be achieved. The invention also finds that in the spinning period of the sterile line, the male flower fertility of the sterile line can be recovered by spraying exogenous gibberellin to tassels, normal selfing propagation can be realized, and the sterile line can be economically and efficiently maintained as a maintainer line. Therefore, the cell nucleus male sterile line created based on the ZmKO1 gene locus has the characteristic of dual purposes and can be used for maize male sterility hybrid seed production, and the invention also provides a technical system for realizing maize two-line method sterile hybrid seed production by utilizing ZmKO1 gene mutation.

The invention relates to a device and a method for ensuring the sterility of objects, products, and / or object- and / or product-contacting components in a cleanroom, wherein an airflow is provided in the cleanroom over a component (20, 30) arranged in the cleanroom to an object, a product, and / or an object- and / or product-contacting component, wherein a cover (10) made of a sterilizable, in particular autoclavable, material can be arranged on the component (20, 30) or upstream of the component (20, 30) such that contamination of the airflow passing over the component (20, 30) is prevented by means of the cover (10). The invention further relates to the use of a cover (10) for ensuring the sterility of objects in a cleanroom.

The invention provides an integrated storage and sterilization device for multi-specification cell culture apparatuses, and belongs to the technical field of crossing of biomedical engineering and laboratory intelligent equipment. The device comprises a multi-specification cell culture appliance information acquisition module, a sterilization parameter intelligent calculation module, a sterilization process execution and real-time monitoring module, a sterilization effect intelligent judgment module and a multi-specification cell culture appliance storage adaptation module. According to the invention, through integration of an integrated architecture and an algorithm, sterile treatment, intelligent management and full-process tracing of multi-specification cell culture apparatuses are finally realized, high requirements of biomedical experiments on sterility, adaptability and controllability of the apparatuses are met, meanwhile, the operation efficiency of a laboratory is improved, and personal errors and pollution risks are reduced.

The invention discloses a freeze-drying automatic conveying system, which relates to the technical field of freeze-drying equipment, and comprises a conveying track which penetrates through a freeze dryer, and the surface of the conveying track is covered with an anti-frosting nano coating. According to the invention, a conveying track covered with an anti-frosting nano coating, an intelligent tray embedded with an RFID chip and a temperature sensor, a transport vehicle carrying visual monitoring, and a bipolar transition cavity module equipped with an electric translation air-tight door and having environment control and sterilization functions are integrated and coordinated by a central controller; full-process automatic and intelligent conveying of materials among freeze-drying pre-treatment, core freeze-drying and post-treatment areas is achieved, then accurate real-time monitoring and full-process tracking of core process parameters are guaranteed, a sterile, low-oxygen and low-humidity environment is rapidly established through an efficient two-stage transition cavity, the pollution risk is reduced, the sterility and stability of products are guaranteed, and the product quality is improved. Therefore, the product quality consistency, the production efficiency and the equipment utilization rate are improved, and the labor cost and the operation risk are reduced.

The invention relates to the technical field of intelligent manufacturing, in particular to sterile directly-prepared blowing-filling-sealing-time-throwing-bottle eye washing liquid and a preparation process thereof. The process comprises the following steps: S1, preparing materials; s2, concentrated preparation; s3, diluting and preparing; s4, sterilizing and filtering; and S5, through a BFS sterile filling process, injecting the to-be-filled eyedrops in the liquid storage tank while performing blow molding on the secondary throwing bottle by using sterilized sterile air, and sealing to obtain the secondary throwing bottle eyedrops. The eye washing liquid in the secondary throwing bottle is prepared from directly prepared eye washing liquid materials in a sterile manner; a multi-stage filtration sterilization and storage time threshold early warning discarding process is adopted in the preparation process, so that the sterile condition in the preparation process is ensured; and meanwhile, a BFS sterile filling process is adopted, so that blowing, filling and sealing are integrated, and sterility is realized.

The invention relates to a male gamete killer gene S19g-A1 of a rice interspecific hybrid sterile S19 seat and application of the male gamete killer gene S19g-A1. The invention provides a rice hybrid sterility related protein S19g-A1. The amino acid sequence of the rice hybrid sterility related protein S19g-A1 is as shown in SEQ ID NO. 2. According to the invention, a key gene S19g-A1 of a rice interspecific hybrid sterile S19 site is researched and identified, function knockout is carried out on African rice S19g-A1, and pollen fertility of a mutant hybrid F1 obtained by carrying out distant hybridization on an obtained African rice s19g-a1 mutant returns to normal. Based on a gene editing technology, the invention develops a method for rapidly creating a hybrid affinity line capable of overcoming sterility of African rice hybrid, effectively eliminates S19 locus mediated interspecific hybrid reproductive disorder, breaks through the bottleneck of rice interspecific hybrid advantage utilization, provides target gene resources for distant hybrid advantage utilization and crop yield improvement, and has a wide application prospect. Good application prospects are realized.

The invention discloses a gene of a rice indica-japonica intersubspecific hybrid sterile locus HS12 and application thereof, and belongs to the field of plant genetic engineering. The locus is positioned at the tail end of a long arm of a chromosome 12, is positioned between molecular markers Sy55 and Sy8, and comprises four fertility related genes iTx1, iTx2, iAd1 and jAd2. By functional knockout or inhibition of expression of the gene iTx1 and / or iTx2, iAd1 and / or jAd2 are / is transferred, a wide-compatibility rice material can be created, meanwhile, a molecular marker for detecting HS12 is further provided and used for filial generation detection and auxiliary breeding, indica-japonica hybrid sterility can be effectively overcome, and the setting rate of offspring is increased.

The invention discloses a ZaSCL3 gene for regulating and controlling plant growth and development as well as an encoding protein and application thereof, and belongs to the technical field of gene engineering. According to the invention, an over-expression vector of the ZaSCL3 gene is constructed and expressed in a wild tomato, so that stamens of a ZaSCL3 over-expression plant are aborted, ovary is spontaneously expanded without pollination, and seedless fruits are generated, which indicates that the over-expression ZaSCL3 gene can realize the effect of inducing male sterility and apomixis of the plant; a key gene resource is provided for molecular breeding and yield character improvement, and the important application value is achieved.

The invention discloses a lettuce LsNP1 gene and application of the lettuce LsNP1 gene in preparation of male sterile plants. Specifically, the invention discloses a protein LsNP1 with an amino acid sequence of SEQ ID NO: 1 and application of a coding gene of the protein LsNP1 in forming a male sterility phenotype of lettuce. Experiments show that the number of pollen grains of the mutant obtained after the LsNP1 gene is knocked out is obviously reduced, the pollen activity is obviously reduced, the pollen grains are abnormal in shape, and anther cannot normally scatter and inseminate during flowering, so that lettuce forms a male sterility phenotype. The male sterile lettuce is successfully created, valuable gene resources are provided for germplasm resource innovation and genetic improvement of the lettuce, a new field is developed for application of the LsNP1 gene, and a new material is provided for application of a lettuce male sterile line. By utilizing the gene and the method, the lettuce male sterile line germplasm can be rapidly created, the cultivation process of a new lettuce variety is accelerated, and the gene and the method have a wide application prospect in the field of agricultural production.

In order to solve the problems of unstable humidity and poor sterilization effect in the prior art, the invention provides a cell incubator and a humidity control method, the cell incubator comprises an incubator inner cavity, an outer door and a sealing structure, the incubator inner cavity is provided with a biological decontamination system, the biological decontamination system comprises an active humidification system and an airflow residue removal system, and the active humidification system is connected with the airflow residue removal system. The active humidifying system comprises a sterilizing agent input port, a water input port and an evaporator for evaporating a sterilizing agent and water, and the evaporator is mounted on the inner wall of the inner cavity of the incubator through an evaporator mounting seat. By means of the efficient sterilization function and the stable sealing structure, the sterile state of the inner cavity can be kept, the purity of the experimental environment is ensured, in addition, the humidifying device further has the capacity of accurately controlling the humidifying amount, humidity control is effectively stabilized, and more ideal experimental conditions are provided.

A sterile and ready-to-use bupivacaine solution is contained in a polymeric bag and is substantially free of any buffer, excipient, and preservative. Advantageously, the solution and the polymeric material allow for autoclaving to sterility without a significant pH drift towards lower pH values and for maintaining active ingredient in solution.

The invention relates to a nucleic acid molecule for detecting a transgenic maize event DBN9641 containing a universal hybrid seed production (UHT) and a detection method of the nucleic acid molecule, and the nucleic acid molecule comprises SEQ ID NO: 1 or a complementary sequence of the SEQ ID NO: 1, and SEQ ID NO: 2 or a complementary sequence of the SEQ ID NO: 2. The corn plant DBN9641 is endowed with a function of keeping sterility of a sterile line in a hybrid seed production technology, so that a castration step in a hybrid seed production process is omitted, manpower and material resources are saved, the operation is simple, and the detection method can accurately and quickly identify whether a biological sample contains DNA molecules of the transgenic corn event DBN9641 or not.

The invention relates to the technical field of plant breeding, in particular to a method for constructing a dominant male sterile line based on CIF1 gene overexpression. The brand-new dominant male sterility gene CIF1 is found, it is verified that the gene has functions in monocotyledonous and dicotyledonous plants, and dominant male sterility resources are enriched; besides, a method capable of manually controlling the fertility recovery of the dominant sterile line is found, a reproductive breeding line for large-scale propagation is obtained, male sterile filial generations can be obtained by hybridizing the breeding line with normal plants, and a new application way is provided for large-scale application of dominant sterility.

Injectable compositions and methods of preparation, as well as therapeutic uses, of induced pluripotent stem cell (iPSC)-derived notochordal cell (iNC)-loaded microgels are provided. Microfluidic on-chip platform can be utilized to prepare microgels (or microgel particles / spheres) formed from block copolymers that exhibit reverse thermal gelation, so as to encapsulate iNCs. Also provided are preconditioned iNC-loaded microgels and iNCs in bulk hydrogel. Cell purity, identity, viability, sterility, and the stability of microencapsulated iNCs have been evaluated. Safety and efficacy of the compositions as therapeutic candidates has been tested via intradiscal injection in animal models of intervertebral disc (IVD) degeneration and discogenic low back pain. Biobehavioral testing, MRI, and immunohistochemical analyses were utilized to evaluate the regenerative potential and reproducibility of the compositions as therapeutic candidate. Single cell RNA sequencing of the treated IVDs may also reveal mechanism of action of the compositions.

The invention discloses an automatic sterility inspection system and method for radiopharmaceuticals, and the system comprises a feeding module which is used for placing medicine bottles, culture bottles and injectors; the bottle opening mechanism is used for opening the medicine bottle; a sample transfer device and an injector control device are arranged on the moving device; the sample transferring device is used for moving the medicine bottles and the culture bottles; the injector control device is used for being connected with an injector and driving a push rod on the injector to move so as to achieve extraction and injection of medicine. The culture detection module is used for placing a culture bottle and culturing the medicine; the culture detection module is provided with a camera for photographing and detecting the medicine in the culture bottle; and a waste box. High automation of the detection process is achieved, opening of medicine bottles and injection and transfer of medicine are all automatically completed in the closed machine body, harm caused by medicine radiation to personnel is effectively avoided, the whole process is high in automation degree, and operation complexity is reduced.

A synthetic expression regulator for a plant gene is disclosed, related to a phenotypic trait of male sterility, and a process for producing male sterile plants using synthetic regulators and use thereof for breeding hybrid crop varieties.

The present invention relates to a liquid dispenser with a tip opening mechanism of the type without preservatives for blow-fill-seal containers that comprises a dual function membrane to control the passage of air and liquid and a pierceable silicone seal that ensures sterility at all times when in the storage position and when opening the container.

Compositions and methods are capable of modulating male fertility in a plant. Compositions comprise polynucleotides and polypeptides, and fragments and variants thereof, which modulate male fertility. Expression cassettes comprise a male-fertility polynucleotide, or fragment or variant thereof, operably linked to a promoter, wherein expression of the polynucleotide modulates the male fertility of a plant. The level and / or activity of a polynucleotide that influences male fertility is modulated in a plant or plant part. Regulatory sequences drive expression in a male-tissue-preferred manner and may be targets to downregulate an operably linked gene. Methods to track mutations that induce nuclear recessive male sterility in subsequent selfing and crossing of wheat lines containing the mutations are also provided. Male-sterile plants may be maintained by pollinating with a maintainer plant.

The present invention discloses a preparation method of an active injectable gel that can withstand irradiation sterilization. The active injectable gel is composed of a collagen gel extracted from an animal source, acellular dermal matrix microparticles, bacterial cellulose, glycerol, and amino acids compounded in a certain proportion, and serves as a filling material that plays an exogenous filling and endogenous collagen regeneration role. Through the protection of glycerol and amino acids and the method of cryopackaging with liquid nitrogen or dry ice, the collagen gel can still maintain its activity and gel properties after being sterilized by Co60 irradiation, and achieve the effects of sterility and virus inactivation. By adding acellular dermal matrix microparticles and bacterial cellulose, the injection force is increased and the action time of the pure collagen gel is effectively extended to achieve a long-term effect.

The invention discloses a KASP molecular marker for identifying a Chinese cabbage nuclear male sterility gene and application of the KASP molecular marker. The invention belongs to the field of molecular biology, and particularly relates to a KASP molecular marker for identifying a Chinese cabbage nuclear male sterility gene and application of the KASP molecular marker. The method for identifying or assisting in identifying the Chinese cabbage fertility comprises the steps that the genotype of an SNP site in a Chinese cabbage genome to be detected is detected, the Chinese cabbage fertility is identified or assisting in identifying according to the genotype, the SNP site is an SNP site on a Chinese cabbage A07 chromosome, the nucleotide type of the SNP site is C or T, and the SNP site is the 1935th nucleotide of SEQ ID No: 4 in a sequence table. The SNP locus related marker can be used for Chinese cabbage male sterility molecular marker assisted breeding, and a new gene resource is provided for Chinese cabbage heterosis utilization.

The invention belongs to the field of plant biotechnology breeding, and relates to an application of a ZmGalAT1 gene and an encoding protein thereof in corn fertility control. According to the invention, a corn male sterility mutant galat1 is obtained by EMS mutagenesis, and a gene ZmGalAT1 for controlling corn male flower development is identified through map-based cloning. The gene is subjected to site-directed mutagenesis in wild type corn through a CRISPR / Cas9 gene editing technology, three galat1 allelic corn male sterility mutants are created, corn male flowers of the mutants are completely aborted, sterile mutants without transgenic ingredients can be obtained through offspring screening, and the mutants are further applied to corn sterile breeding and seed production. The invention also designs functional molecular markers for three allelic male sterile mutants of GalAT1, and has important application value in maize male sterile line cultivation, sterile hybrid seed production and molecular marker-assisted selection.

This invention belongs to the field of molecular genetics and discloses a method and application for controlling fish fertility using in vivo gene editing technology. The applicant, for the first time, cloned a carp-specific U6 promoter and used this promoter to construct the U6 gene. MOVIE gRNA transgenic vectors and water The Cas9 vector allows for the establishment of an in vivo editing system. This strategy enables the heritability of sterility, a reproductive control trait, through hybridization of fertile parents to produce sterile offspring. The operation is highly efficient, simple, and environmentally friendly. Furthermore, since high-copy-rate short, scattered repetitive sequences exist in all fish species, this strategy has broad applicability across species.