Combined detection kit for seminal plasma

A kit and detection reagent technology, applied in the field of biomedical testing and determination, can solve problems such as complex operation steps, many sample addition procedures, and large human errors, and achieve the effects of easy interpretation of results, multiple detection indicators, and convenient operation

Active Publication Date: 2014-04-30
AUTOBIO DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method for detecting neutral α-glucosidase in seminal plasma is chemical colorimetry, which is mostly manual or semi-automatic, with many sample addition procedures and complicated operation steps. Colorimetric measurements are carried out one by one, and then the results are calculated according to the standard curve, which is time-consuming and labor-intensive, and the human error is also relatively large

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The seminal plasma joint inspection kit of the present invention includes a sample diluent, a chromogenic solution, and a detection card body provided with nine reaction wells. Solid reagents for phospholipid bodies, citric acid, zinc, acid phosphatase, elastase, fructose and neutral α-glucosidase, the solid reagent is made of cotton pulp paper, filter paper, glass fiber or plastic, etc. The specific preparation method of the reagent pads in the nine reaction wells is as follows:

[0034] 1. Solid reagent pad for detecting pH value: Take 10 μL of the solution prepared by 0.5~100 g / L bromothymol blue sodium salt and 20 times of purified water, put it on the reagent pad carrier with a diameter of 5.5 mm, dehumidify and dry (Temperature 18~25℃, humidity ≤30%);

[0035] 2. Solid reagent pad for detection of leukocyte esterase: prepared from Tris-HCl buffer, sugar and 5-bromo-4-chloro-3-indole acetate solution, coated on the carrier, dehumidified and dried (temperature 18 ...

Embodiment 2

[0065] The seminal plasma joint inspection kit of the present invention includes a sample diluent, a chromogenic solution, and a detection card body provided with nine reaction wells. Solid reagents for phospholipid bodies, citric acid, zinc, acid phosphatase, elastase, fructose and neutral α-glucosidase, the solid reagent is made of cotton pulp paper, filter paper, glass fiber or plastic, etc. The specific preparation method of the reagent pads in the nine reaction wells is as follows:

[0066] 1. Solid reagent pad for detecting pH value: Take 10 μL of the solution prepared by 0.5~100 g / L bromothymol blue sodium salt and 20 times of purified water, put it on the reagent pad carrier with a diameter of 5.5 mm, dehumidify and dry (Temperature 18~25℃, humidity ≤30%);

[0067] 2. Solid reagent pad for detection of leukocyte esterase: prepared from Tris-HCl buffer, sugar and 5-bromo-4-chloro-3-indole acetate solution, coated on the carrier, dehumidified and dried (temperature 18 ...

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Abstract

The invention discloses a combined detection kit for seminal plasma. The nine major indexes of pH value, leukocyte esterase, lecithin body, citric acid, zinc, acid phosphatase, elastase, fructose and neutral alpha-glucosidase in the seminal plasma can be rapidly, accurately, simply, conveniently and practically detected at one time in a combined way, functions of glands such as the prostate, the epididymis and the vesicula seminalis can be comprehensively evaluated, detailed related information is provided for the clinical diagnosis and treatment of caused related diseases such as prostatitis, reproductive system inflammation, sexual dysfunction and sterility infertility, a market gaps are filled, and the combined detection kit is easy to clinically popularize.

Description

technical field [0001] The invention relates to the technical field of biomedical testing and measurement, in particular to a seminal plasma joint detection kit for in vitro diagnosis. Background technique [0002] Seminal plasma is a mixture of secretions from male accessory gonads, which has the functions of transporting sperm, providing sperm nutrition, and stimulating sperm vitality. Analysis of seminal plasma can directly and objectively evaluate male fertility, find out the cause of male infertility, provide evidence for the quality of sperm for artificial insemination, and also detect inflammation and tumors in the reproductive system. Objectively evaluate the function of the system and provide assistance for the diagnosis, treatment and prognosis monitoring of related diseases. [0003] The content of white blood cells in semen is an important indicator for diagnosing reproductive system inflammatory response and infertility. Clinically, the number of white blood ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52
CPCC12Q1/34C12Q1/42C12Q1/44C12Q2334/52G01N33/5091
Inventor 张中洲娄斌曹艺甜刘功成付光宇李晓霞王胜启陆莹
Owner AUTOBIO DIAGNOSTICS CO LTD
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