83results about How to "Easy to read results" patented technology

The invention discloses a quick test paper for detecting enterovirus and a method for preparing the same. The test paper detects enterovirus EV71 and Coxsackievirus A16 viruses in a sample by the immunochromatography adopting marking by colorful particles. The quick test strip is formed by sequentially overlapping and bonding a sample adsorption liquid layer, a colorful particle storing pad, a cellulose nitrate membrane and a water adsorption board on a bottom board, wherein the colorful particle storing pad is coated with a monoclonal antibody with a colorful particle mark; and the cellulose nitrate membrane is provided with a detection line sprayed by the monoclonal antibody of the EV71 and / or Coxsackievirus A16 and a control line sprayed by the polyclonal antibody of anti-mouse IgG. The test paper can quickly detects the EV71 and Coxsackievirus A16 viruses in the detected sample at the same time or respectively, finds the epidemic situation caused by the virus infection as early as possible and has the advantages of being easily operated, quickly getting results, avoiding special operators and the like.

The invention discloses a CRISPR / Cas12a kit and a detection method for rapid detection of nucleic acid of mycoplasma pneumoniae. The CRISPR / Cas12a kit comprises a CRISPR / Cas12a detection system, wherein the CRISPR / Cas12a detection system comprises specific crRNA aiming at mycoplasma pneumoniae, a CRISPR / Cas12a protein and a single-stranded DNA reporting system, wherein the specific crRNA is any one or more designed and synthesized according to mycoplasma pneumoniae dsDNA sites, and the single-stranded DNA reporting system comprises am ssDNA FQ reporter used for fluorescence detection of a microplate reader and / or an ssDNA DB reporter used for immunogold test strip detection. According to the invention, CRISPR / Cas12a is firstly adopted to detect the nucleic acid sequence of mycoplasma pneumoniae, and has the advantages of high sensitivity, strong specificity, short time consumption, no dependence on large-scale experimental equipment and the like.

Data can be scanned using a network managed appliance. The network managed appliance may integrate commercial hardware elements connected through a basic or simplified operating system environment expressly developed for the appliance, thus being more malware resistant and less vulnerable to attacks from the scanned data or other sources. The network managed appliance may be a self-contained apparatus with an integrated chassis, designed and configured as “single-purpose” device. Such appliances may be connected to an appliance management network including central management servers in communication with appliances in remote locations. The central management servers may ensure that scanning software and the definitions lists for each of the appliances are current and match an enterprise-approved configuration.

The invention discloses a combined detection kit for seminal plasma. The nine major indexes of pH value, leukocyte esterase, lecithin body, citric acid, zinc, acid phosphatase, elastase, fructose and neutral alpha-glucosidase in the seminal plasma can be rapidly, accurately, simply, conveniently and practically detected at one time in a combined way, functions of glands such as the prostate, the epididymis and the vesicula seminalis can be comprehensively evaluated, detailed related information is provided for the clinical diagnosis and treatment of caused related diseases such as prostatitis, reproductive system inflammation, sexual dysfunction and sterility infertility, a market gaps are filled, and the combined detection kit is easy to clinically popularize.

The invention relates to a histoplasma capsulatum infectious molecular diagnosis reagent kit based on the recombinase and polymerase amplification technological principle and application thereof. A primer is designed for an M antigen gene of an rDNA area of a histoplasma capsulatum capsule variation distributed according to the most common advantages of histoplasma capsulatum in clinical in China, the primer reacts with DNA of 30 histoplasma capsulatum standard strains, clinic and environment separated strains and 50 strains in relevant species according to an RPA system, and it is proved that the primer has excellent specificity. It is also proved that the primer has very high sensitivity through sensitivity testing. Accordingly, the primer can be used for early clinical diagnosis of histoplasma capsulatum infection, the problems that in clinical, histoplasma capsulatum infection confirmed diagnosis period is long and requirements for detection staff skills and laboratory platform conditions are high are solved, the primer can be prepared into the reagent kit, and convenience is provided for early diagnosis and timely treatment of clinical histoplasma capsulatum infection.

The invention discloses primers and probes and a kit for detecting seven mutation types of a human RET gene. According to each group of the seven groups (RM1-RM7) of the primers and probes, the mutation upstream ARMS primer can be specially combined with a corresponding mutation sequence of the mutation upstream ARMS primer to amplify the mutation sequence, the mutation downstream primer can be combined with a conserved sequence of the RET gene, the detection probe can be combined with an amplified fragment, and the blocking probe can be specially combined with a wild type sequence corresponding to a mutation locus to inhibit wild type nonspecific amplification. Accordingly, by adopting a specific mutation primer and probe blocking technology, the seven mutation types of the RET gene can be accurately detected; through the established kit of a real-time fluorescence PCR amplification reaction system, rapid detection on the RET gene mutation is facilitated, operation is easy, and a result is easy to read; the detection method is high in sensitivity, and 50-copy mutation can be stably detected; the specificity is good, no nonspecific amplification is generated in 20-ng wild type genome DNA, and the detectability reaches up to 1%.

The invention discloses a probe, a primer and a kit for detecting seven kinds of mutations of human NRAS genes, wherein in seven groups of primers and probes from NM1 to NM7, the mutation primer in each group can be combined with a conserved sequence of the NRAS genes; the mutation ARMS primers can realize the specific binding with the corresponding mutation sequences; the mutation sequences are amplified; the detection probe can be combined with an amplification segment; the specific binding of the probe with the wild sequence corresponding to the mutation site is blocked; and the wild nonspecific amplification is inhibited. The specific mutation primer and probe blocking technology is used; the seven kinds of mutation types of the NRAS genes can be accurately detected; the built kit for a real-time fluorescent PCR (Polymerase Chain Reaction) amplification reaction system can be used for conveniently and fast detecting the mutations of the NRAS genes; the operation is simple; the result can be easily read; the sensitivity of a detection method is high; 50 copy mutations can be stably detected; the specificity is good; the 20ng wild genome DNA (Deoxyribose Nucleic Acid) does not have specific amplification; and the detection capability is as high as 1 percent.

The invention discloses an immunochromatographic test strip for rapidly detecting acute pancreatitis and a preparation method thereof. The test strip is formed by sticking a sample pad, a glass fiber membrane labeling pad, a nitrocellulose coating membrane and absorbent paper to a substrate in sequence through lap joint, wherein a highly specific monoclonal antibody of trypsinogen-2 labeled by a biotin-avidin-colored latex complex is coated on the glass fiber membrane labeling pad; the nitrocellulose coating membrane comprises a detection region and a control region; the detection region is coated by another specific monoclonal antibody of trypsinogen-2 with epitope different from that of the labeled monoclonal antibody on the glass fiber membrane labeling pad; and the control region is coated by an anti-mouse antibody. Compared with radioimmunoassay and enzyme linked immunosorbent assay (ELISA), the test strip has the advantages of safety, simpleness and convenience in operation, suitability for single human / sample detection, rapidness and the like, dispenses with special skills, is free of trauma, is easy in result reading and can realize timely in-situ and family self-detectionof acute pancreatitis.

The invention discloses a probe, a primer and a kit for detecting 7 mutations of a human CKIT gene. 7 groups of primers and probes CM1 to CM7 are provided; in each group, a mutation forward primer can be combined with a CKIT gene conserved sequence, and a mutation reverse ARMS primer can be specifically combined with a corresponding mutation sequence so as to amplify the mutation sequence; the detection probes can be combined with amplified fragments, and blocking probes can be specifically combined with a wild-type sequence corresponding to a mutation site so as to inhibit wild-type non-specific amplification. According to the probe, the primer and the kit which are disclosed by the invention, a specific mutation primer and probe blocking technology is adopted; 7 mutations of the CKIT gene can be accurately detected; the established real-time fluorescence PCR (Polymerase Chain Reaction) amplified reaction system kit is convenient to rapidly detect the mutations of the CKIT gene, is simple to operate, and is easy in reading of a result; a detection method is high in sensitivity, and 50-copy mutants can be stably detected out; specificity is good, 20ng wild-type genome DNA has no phenomenon of nonspecific amplification, and detectability reaches 1 percent.

The invention relates to a histoplasma infection molecular diagnosis kit based on loop-mediated isothermal amplification (LAMP) technique principles and application thereof. By selecting appropriate target sequences, an appropriate LAMP primer set is obtained according to the ITS segment and M antigen gene. The primer set has the advantages of excellent specificity and high sensitivity, and thus, is suitable for early infection diagnosis of histoplasma and detection and identification of histoplasma in the sample. The primer set for diagnosing histoplasma is quick and simple, is high in operability and convenient for result interpretation, solves the problems of long time cycle for clinical histoplasma infection diagnosis, high requirements for detection personnel technology and laboratory platform conditions, and the like, can be prepared into a kit, and is suitable for wide popularization and application.

The invention relates to primers capable of early diagnosis of cryptococcus infections and application thereof. The primers are designed by using an IGS1 sequence of the most common clinical strain of newborn cryptococcus in the world-H99 strain as a target sequence by utilizing LAMP (loop-mediated isothermal amplification) professional primer design software. The experiment on effectiveness, specificity and sensitivity proves that the primers can be used for early diagnosis of cryptococcus infections, have the advantages of high speed, simpleness, strong operability and convenient result identification, solve the problems of long time period for diagnosing cryptococcus infections, low requirements for detection personnel technique and laboratory platform conditions and the like, can be made into a kit, and provide convenience for early diagnosis and timely treatment of cryptococcus infections.

The invention discloses an RT-LAMP (reverse transcription and loop-mediated isothermal amplification) detection primer group, an RT-LAMP detection kit and an RT-LAMP detection method for a simian immunodeficiency virus. The detection primer group comprises a pair of outer primers, a pair of loop primers and a pair of inner primers. The detection kit comprises the primer group, RT-LAMP reaction liquid, Bst DNA polymerase, reverse transcriptase as well as negative control and positive control. The detection method comprises the following steps: extracting to-be-detected virus RNA; conducting reverse transcription on the RNA under the action of revertase; then, amplifying a sample template by virtue of six specific primers and one Bst DNA polymerase having strand displacement activity at 63-65 DEG C; and judging whether a to-be-detected sample contains the simian immunodeficiency virus (SIV) RNA by observing whether a reaction tube solution becomes muddy or not with naked eyes or performing electrophoresis to analyze whether a ladder exists or not. The detection primer group, the detection kit and the detection method provided by the invention have the advantages of being highly specific, high in sensitivity, rapid and efficient, simple and convenient to operate, easy in result reading and the like, and are suitable for popularization and application in grassroots animal epidemic disease monitoring units and experimental monkey breeding enterprises in remote areas.

A recording apparatus is provided. The recording apparatus is a recording apparatus having a scanning function, and is excellent in operability when reading a document the size of which is larger than the size of a document reading area. A printer includes a scanner unit which reads a document; and a recording portion which records information read by the scanner unit. In the recording portion, the maximum recordable size of a paper sheet is greater than a size of a document reading area (30a) in the scanner unit. At a base end side of a cover (4) which opens and closes the document reading area (30a), a gap (35) which passes the document which is pushed out from the document reading area (30a) and of which the size is greater than the size of the document reading area (30a) to an outer side of the scanner unit is formed.

The invention discloses a multi-target microarray chip full-automatic hybrid cleaning dyeing system comprising a multi-target microarray chip card box and a full-automatic hybrid cleaning dyeing system. The automatic hybrid cleaning dyeing system comprises a sample and reagent processing module, an incubation reaction module and a fluid control module. The disposable visual multi-target microarray chip card box adopts a fixed closed system, so that the risk of pollution is reduced, results are convenient to read, and fluid operation is convenient in a process that an instrument automatically performs a reaction; and the full-automatic hybrid cleaning dyeing system accelerates the experimental speed, and improves repeatability and consistency of experimental results.