83results about How to "Easy to read results" patented technology

A pattern recognition apparatus for detecting a predetermined pattern contained in an input signal is provided with plural detecting processing parts and for detecting respectively different features for a same input, plural integrating processing parts for spatially integrating, for each process results, the features detected by the plural detecting processing parts, plural detecting memories for retaining the process results of the detecting processing parts, plural integrating memories for retaining the process results of the integrating processing parts, a global data line 1030 to which all the predetermined detecting processing parts and all the predetermined integrating memories are connected at a certain timing, and plural local data lines each of which is connected to a predetermined set of the detecting processing parts, the integrating processing parts and the detecting memory.

The invention provides a hemoglobin, hemoglobin-haptoglobin composite and transferrin joint examination kit. The hemoglobin, hemoglobin-haptoglobin composite and transferrin joint examination kit comprises an upper shell, a lower shell, an immunostrip, test paper and the like; a gold-labeled composite containing a hemoglobin monoclonal antibody, a hemoglobin-haptoglobin composite monoclonal antibody and a transferrin monoclonal antibody is scribed on a nitrocellulose membrane; 3 test lines, a quality control line (15) and a gold-labeled composite membrane scribing line (8) are arranged on the nitrocellulose membrane in parallel. The invention further provides a preparation method and a detection method of the hemoglobin, hemoglobin-haptoglobin composite and transferrin joint examination kit. The hemoglobin, hemoglobin-haptoglobin composite and transferrin joint examination kit is convenient and simple in operation, stable in performance and accurate in result, has a relatively good reference value for early diagnosis and identification of colorectal cancer or colon cancer tumor or other tumors with lower gastrointestinal bleeding symptoms in clinic, significantly improves the positive detection rate of digestive hemorrhagic diseases, is suitable for clinical hospital examination and household self-examination, and provides measures for large-scale general examination of such diseases.

The invention discloses a Cpf1 reagent kit for quickly detecting nucleic acid of an African swine fever virus. The Cpf1 reagent kit comprises a Cpf1 detection system suitable for quickly detecting theAfrican swine fever virus, and an immune colloidal gold test strip, wherein the Cpf1 detection system comprises specific crRNA protein, specific Cpf1 protein and a single-chain DNA(ssDNA) reporting system in accordance with a p72 gene of the African swine fever virus, the specific crRNA is one or more of crRNAs from ASFV P72 crRNA1 to ASFV P72 crRNA10, and the sequence of the specific crRNA is SEQ NO.4 to SEQ NO.13; and the single-chain DNA(ssDNA) reporting system comprises ssDNA FQreporter for fluorescence detection of a microplate reader and / or ssDNA DB reporter for detecting the immune colloidal gold test strip. According to the Cpf1 reagent kit disclosed by the invention, for the first time, the Cpf1 is used for detecting the African swine fever virus, and has the advantages of beinghigh in sensitivity, high in specificity, short in time consumption, high in flux, independent of large-scale experiment equipment and the like. The advantages enable a detection method based on the immune colloidal gold test strip developed by the invention to be conveniently used in basic laboratories and breeding enterprises to be used for performing detection, identification and diagnosis on basic quick detection of the African swine fever.

The invention discloses a quick test paper for detecting enterovirus and a method for preparing the same. The test paper detects enterovirus EV71 and Coxsackievirus A16 viruses in a sample by the immunochromatography adopting marking by colorful particles. The quick test strip is formed by sequentially overlapping and bonding a sample adsorption liquid layer, a colorful particle storing pad, a cellulose nitrate membrane and a water adsorption board on a bottom board, wherein the colorful particle storing pad is coated with a monoclonal antibody with a colorful particle mark; and the cellulose nitrate membrane is provided with a detection line sprayed by the monoclonal antibody of the EV71 and / or Coxsackievirus A16 and a control line sprayed by the polyclonal antibody of anti-mouse IgG. The test paper can quickly detects the EV71 and Coxsackievirus A16 viruses in the detected sample at the same time or respectively, finds the epidemic situation caused by the virus infection as early as possible and has the advantages of being easily operated, quickly getting results, avoiding special operators and the like.

Data can be scanned using a network managed appliance. The network managed appliance may integrate commercial hardware elements connected through a basic or simplified operating system environment expressly developed for the appliance, thus being more malware resistant and less vulnerable to attacks from the scanned data or other sources. The network managed appliance may be a self-contained apparatus with an integrated chassis, designed and configured as “single-purpose” device. Such appliances may be connected to an appliance management network including central management servers in communication with appliances in remote locations. The central management servers may ensure that scanning software and the definitions lists for each of the appliances are current and match an enterprise-approved configuration.

The invention discloses a combined detection kit for seminal plasma. The nine major indexes of pH value, leukocyte esterase, lecithin body, citric acid, zinc, acid phosphatase, elastase, fructose and neutral alpha-glucosidase in the seminal plasma can be rapidly, accurately, simply, conveniently and practically detected at one time in a combined way, functions of glands such as the prostate, the epididymis and the vesicula seminalis can be comprehensively evaluated, detailed related information is provided for the clinical diagnosis and treatment of caused related diseases such as prostatitis, reproductive system inflammation, sexual dysfunction and sterility infertility, a market gaps are filled, and the combined detection kit is easy to clinically popularize.

The invention discloses a test strip for fast detecting premature rupture of fetal membranes, which is formed in a way that a sample pad, a glass fiber membrane, a coating membrane and absorbent paper are sequentially overlapped and pasted on a substrate, wherein the glass fiber membrane is coated with a high-specificity monoclonal antibody of latex particle-labeled insulin-like growth factor binding protein-1 (IGFBP-1); and the coating membrane comprises a detection area coating an anti-IGFBP-1 specific monoclonal antibody and a control area coating an anti-mouse antibody. Compared with the radioimmunoassay method and the enzyme-linked immunosorbent assay (ELISA) method, the test strip disclosed by the invention has the advantages of operational safety, fast detection and the like, is simple and convenient and is applicable to individual detection. The test strip disclosed by the invention can be conveniently and fast operated by people without professional skills, can not cause damages and can realize timely on-site and household self detection of premature rupture of fetal membranes; and the result can be read easily.

The invention discloses a primer, a probe and a kit for detecting a mutation of a human JAK2 gene V617F. The primer comprises a mutation forward primer JW1-F and a mutation reverse ARMS primer JM1-R, and the probe comprises a detection probe JW1-P and a blocking probe JW1-B, wherein the mutation forward primer JW1-F is combined with a JAK2 gene conserved sequence; the mutation reverse ARMS primer JM1-R is specifically combined with a V617F (1849G>T) site mutation sequence to selectively amplify the mutation sequence; a 5minute end of the detection probe JW1-P is marked with an FAM (carboxy fluorescein) signal; a 3minute end of the detection probe is marked with an MGB (Minor Groove Binder); the detection probe JW1-P can be combined with an amplified fragment; a 5minute end of the blocking probe JW1-B is double-deoxidized and modified; a 3minute end of the blocking probe JW1-B is marked with the MGB; the blocking probe JW1-B can be specifically combined with a V617 site wild-type sequence to inhibit wild-type nonspecific amplification. The primer and the probe which are disclosed by the invention are high in specificity and good in sensitivity, and have high detectability of 1 percent. The kit prepared from the primer and the probe is accurate in detection result and easy in reading of the detection result, is simple and rapid to operate, and is wide in application range.

The invention relates to a histoplasma capsulatum infectious molecular diagnosis reagent kit based on the recombinase and polymerase amplification technological principle and application thereof. A primer is designed for an M antigen gene of an rDNA area of a histoplasma capsulatum capsule variation distributed according to the most common advantages of histoplasma capsulatum in clinical in China, the primer reacts with DNA of 30 histoplasma capsulatum standard strains, clinic and environment separated strains and 50 strains in relevant species according to an RPA system, and it is proved that the primer has excellent specificity. It is also proved that the primer has very high sensitivity through sensitivity testing. Accordingly, the primer can be used for early clinical diagnosis of histoplasma capsulatum infection, the problems that in clinical, histoplasma capsulatum infection confirmed diagnosis period is long and requirements for detection staff skills and laboratory platform conditions are high are solved, the primer can be prepared into the reagent kit, and convenience is provided for early diagnosis and timely treatment of clinical histoplasma capsulatum infection.

The invention discloses primers and probes and a kit for detecting seven mutation types of a human RET gene. According to each group of the seven groups (RM1-RM7) of the primers and probes, the mutation upstream ARMS primer can be specially combined with a corresponding mutation sequence of the mutation upstream ARMS primer to amplify the mutation sequence, the mutation downstream primer can be combined with a conserved sequence of the RET gene, the detection probe can be combined with an amplified fragment, and the blocking probe can be specially combined with a wild type sequence corresponding to a mutation locus to inhibit wild type nonspecific amplification. Accordingly, by adopting a specific mutation primer and probe blocking technology, the seven mutation types of the RET gene can be accurately detected; through the established kit of a real-time fluorescence PCR amplification reaction system, rapid detection on the RET gene mutation is facilitated, operation is easy, and a result is easy to read; the detection method is high in sensitivity, and 50-copy mutation can be stably detected; the specificity is good, no nonspecific amplification is generated in 20-ng wild type genome DNA, and the detectability reaches up to 1%.

The invention discloses a probe, a primer and a kit for detecting seven kinds of mutations of human NRAS genes, wherein in seven groups of primers and probes from NM1 to NM7, the mutation primer in each group can be combined with a conserved sequence of the NRAS genes; the mutation ARMS primers can realize the specific binding with the corresponding mutation sequences; the mutation sequences are amplified; the detection probe can be combined with an amplification segment; the specific binding of the probe with the wild sequence corresponding to the mutation site is blocked; and the wild nonspecific amplification is inhibited. The specific mutation primer and probe blocking technology is used; the seven kinds of mutation types of the NRAS genes can be accurately detected; the built kit for a real-time fluorescent PCR (Polymerase Chain Reaction) amplification reaction system can be used for conveniently and fast detecting the mutations of the NRAS genes; the operation is simple; the result can be easily read; the sensitivity of a detection method is high; 50 copy mutations can be stably detected; the specificity is good; the 20ng wild genome DNA (Deoxyribose Nucleic Acid) does not have specific amplification; and the detection capability is as high as 1 percent.

The invention discloses an immunochromatographic test strip for rapidly detecting acute pancreatitis and a preparation method thereof. The test strip is formed by sticking a sample pad, a glass fiber membrane labeling pad, a nitrocellulose coating membrane and absorbent paper to a substrate in sequence through lap joint, wherein a highly specific monoclonal antibody of trypsinogen-2 labeled by a biotin-avidin-colored latex complex is coated on the glass fiber membrane labeling pad; the nitrocellulose coating membrane comprises a detection region and a control region; the detection region is coated by another specific monoclonal antibody of trypsinogen-2 with epitope different from that of the labeled monoclonal antibody on the glass fiber membrane labeling pad; and the control region is coated by an anti-mouse antibody. Compared with radioimmunoassay and enzyme linked immunosorbent assay (ELISA), the test strip has the advantages of safety, simpleness and convenience in operation, suitability for single human / sample detection, rapidness and the like, dispenses with special skills, is free of trauma, is easy in result reading and can realize timely in-situ and family self-detectionof acute pancreatitis.

The invention discloses a probe, a primer and a kit for detecting 7 mutations of a human CKIT gene. 7 groups of primers and probes CM1 to CM7 are provided; in each group, a mutation forward primer can be combined with a CKIT gene conserved sequence, and a mutation reverse ARMS primer can be specifically combined with a corresponding mutation sequence so as to amplify the mutation sequence; the detection probes can be combined with amplified fragments, and blocking probes can be specifically combined with a wild-type sequence corresponding to a mutation site so as to inhibit wild-type non-specific amplification. According to the probe, the primer and the kit which are disclosed by the invention, a specific mutation primer and probe blocking technology is adopted; 7 mutations of the CKIT gene can be accurately detected; the established real-time fluorescence PCR (Polymerase Chain Reaction) amplified reaction system kit is convenient to rapidly detect the mutations of the CKIT gene, is simple to operate, and is easy in reading of a result; a detection method is high in sensitivity, and 50-copy mutants can be stably detected out; specificity is good, 20ng wild-type genome DNA has no phenomenon of nonspecific amplification, and detectability reaches 1 percent.

The invention discloses a Cpf1 kit for rapid detection of hereditary deafness pathogenic gene SLC26A4 mutation and a detection method thereof. The Cpf1 kit comprises a Cpf1 detection system. The Cpf1detection system comprises: a specific crRNA for SLC26A4, a Cpf1 protein and a single-stranded DNA reporting system. The specific crRNA is any one or more designed and synthesized for mutation sites.The single-stranded DNA reporting system comprises an ssDNA FQ reporter for fluorescence detection by an enzyme-labeled instrument and / or an ssDNA DB reporter for immune colloidal gold strip detection. By detecting SLC26A4 gene mutation sites with the Cpf1 for the first time, the kit of the invention has the advantages of high sensitivity, strong specificity, low time consumption, no dependence onlarge-scale experimental equipment and the like.

The invention relates to the technical field of gene polymorphism detection, and in particular relates to a sequencing primer, a kit and a detection method for detecting CYP2C19 gene polymorphism, which aim at overcoming the defects that a DNA microarray chip method for detecting CYP2C19 gene polymorphism is long in consumed time, relatively high in cost and inflexible in use in the prior art. The sequencing primer provided by the invention is the one for detecting CYP2C19*17 gene polymorphism, and the 3'-end base sequence of the sequencing primer is -TTGTGTCTTCTGTTCTCAA-3'. The kit provided by the invention is short in detection time, low in detection cost, flexible in use and very high in detection precision. The detection method provided by the invention is easy in operation.

The invention discloses primers and probes and a kit for detecting five mutation types of a human PIK3CA gene. According to each group of the five groups (PIM1-PIM5) of the primers and probes, the mutation primer can be combined with a conserved sequence of the PIK3CA gene, a mutation ARMS primer can be specially combined with a corresponding mutation sequence of the mutation ARMS primer to amplify the mutation sequence, the detection probe can be combined with an amplified fragment, and the blocking probe can be specially combined with a wild type sequence corresponding to a mutation locus to inhibit wild type nonspecific amplification. Accordingly, by adopting a specific mutation primer and probe blocking technology, the five mutation types of the PIK3CA gene can be accurately detected; through the established kit of a real-time fluorescence PCR amplification reaction system, rapid detection on the PIK3CA gene mutation is facilitated, operation is easy, and a result is easy to read; the detection method is high in sensitivity, and 50-copy mutation can be stably detected; the specificity is good, no nonspecific amplification is generated in 20-ng wild type genome DNA, and the detectability reaches up to 1%.

The invention relates to a specific crRNA, probe, detection system and method for detecting a novel coronavirus SARS-CoV-2 variant based on CRISPR-Cas12a method. The specific crRNA for identifying an L subtype and an S subtype is Orf8-crRNA, and the specific crRNA for identifying variation sites of L5F, D80A, D215G, R246I, Y453F, N501Y, A570D, D614G, A701V, T716I, S982A, P1263L, K417N, L452R and E484Q of SARS-CoV-2 S protein genes is S-L5F-crRNA, S-D80A-crRNA, S-D215G-crRNA, S-R246I-crRNA, S-Y453F-crRNA, S-N501Y-crRNA, S-A570D-crRNA, S-D614G-crRNA, S-A701V-crRNA, S-T716I-crRNA, S-S982A-crRNA, S-P1263L-crRNA, S-K417N-crRNA, S-L452R-crRNA and S-E484Q-crRNA. The nucleotide sequence of the probe is SEQ ID NO. 14. The detection system comprises crRNA, the probe, ribozyme-free water and a buffer solution buffer 2.1. The specific crRNA, the probe, the detection system and the method can accurately detect the SARS-CoV-2 variants, do not need to depend on expensive instruments and professional technicians, are short in detection time, and have the advantages of high specificity, high sensitivity, rapidness, high efficiency, simplicity and convenience in operation, readily readable results and the like.

The invention relates to a histoplasma infection molecular diagnosis kit based on loop-mediated isothermal amplification (LAMP) technique principles and application thereof. By selecting appropriate target sequences, an appropriate LAMP primer set is obtained according to the ITS segment and M antigen gene. The primer set has the advantages of excellent specificity and high sensitivity, and thus, is suitable for early infection diagnosis of histoplasma and detection and identification of histoplasma in the sample. The primer set for diagnosing histoplasma is quick and simple, is high in operability and convenient for result interpretation, solves the problems of long time cycle for clinical histoplasma infection diagnosis, high requirements for detection personnel technology and laboratory platform conditions, and the like, can be prepared into a kit, and is suitable for wide popularization and application.

The invention relates to primers capable of early diagnosis of cryptococcus infections and application thereof. The primers are designed by using an IGS1 sequence of the most common clinical strain of newborn cryptococcus in the world-H99 strain as a target sequence by utilizing LAMP (loop-mediated isothermal amplification) professional primer design software. The experiment on effectiveness, specificity and sensitivity proves that the primers can be used for early diagnosis of cryptococcus infections, have the advantages of high speed, simpleness, strong operability and convenient result identification, solve the problems of long time period for diagnosing cryptococcus infections, low requirements for detection personnel technique and laboratory platform conditions and the like, can be made into a kit, and provide convenience for early diagnosis and timely treatment of cryptococcus infections.

The invention relates to a ball pin assembly torsion test marking device and a test method, and belongs to the technical field of ball pin torsion test. The ball pin assembly torsion test marking device comprises a platform, a limit pre-tightening mechanism which is arranged above the platform and acts on a knuckle bearing sleeve, and a power part which is arranged below the platform and acts on aball pin, the ball pin is connected with the power part through a torque meter. According to the limiting pre-tightening mechanism, the clamping mechanism acts on the knuckle bearing sleeve to simulate the pre-tightening state of the knuckle bearing sleeve during installation, and the technical problems that in the prior art, a ball pin torsion detection technology of a ball pin assembly is immature, the torsion detection efficiency is low, and the detection precision is poor are solved.

The invention discloses an RT-LAMP (reverse transcription and loop-mediated isothermal amplification) detection primer group, an RT-LAMP detection kit and an RT-LAMP detection method for a simian immunodeficiency virus. The detection primer group comprises a pair of outer primers, a pair of loop primers and a pair of inner primers. The detection kit comprises the primer group, RT-LAMP reaction liquid, Bst DNA polymerase, reverse transcriptase as well as negative control and positive control. The detection method comprises the following steps: extracting to-be-detected virus RNA; conducting reverse transcription on the RNA under the action of revertase; then, amplifying a sample template by virtue of six specific primers and one Bst DNA polymerase having strand displacement activity at 63-65 DEG C; and judging whether a to-be-detected sample contains the simian immunodeficiency virus (SIV) RNA by observing whether a reaction tube solution becomes muddy or not with naked eyes or performing electrophoresis to analyze whether a ladder exists or not. The detection primer group, the detection kit and the detection method provided by the invention have the advantages of being highly specific, high in sensitivity, rapid and efficient, simple and convenient to operate, easy in result reading and the like, and are suitable for popularization and application in grassroots animal epidemic disease monitoring units and experimental monkey breeding enterprises in remote areas.

The invention discloses a reagent set, a kit and a detection method for detecting fungal infection, and relates to the field of biotechnology. The reagent set comprises one of the following reagents or a combination of two of the following reagents: a PCR buffer and a complex enzyme preparation. The PCR buffer contains the following components: potassium acetate, MgCl2, glycerol, DMSO, Tween-20, and PEG-200. The complex enzyme preparation contains the following components: snail enzyme, lysozyme, papain and laccase. The reagent set can directly detect samples such as blood without the steps such as nucleic acid extraction and purification, and has the advantages of high sensitivity, good specificity and short detection time.

The invention discloses a horizontal measuring tool. The horizontal measuring tool comprises a base, a transparent casing, a laser transmitter and a refraction liquid, wherein the transparent casing is fixedly arranged on the base and provided with a hollow space; scales are formed on the transparent casing; the laser transmitter is vertically positioned in the hollow space; the refraction liquidis encapsulated in the hollow space; in addition, when the refraction liquid is vertically placed, the laser transmitter is immersed into the refraction liquid; the refraction liquid is a transparentliquid. Whether the horizontal measuring tool disclosed by the invention is horizontal or not is judged by refraction of the refraction liquid to light of the laser transmitter; only when a measuringsurface is horizontal, the light can vertically penetrate through liquid surface, and otherwise the light refracts when penetrating through the liquid level; the light irradiates the scales of the transparent casing to beneficially read the result; in addition, a refractive index of the refraction liquid is fixed and not easily and externally disturbed. Moreover, an inclination angle of the surface where the base is located can be read.

The invention discloses probes, primers and reagent kits for detecting five mutations of a human gene TP53. According to five primers and probes for TM1-TM5, each mutation primer can be combined with a conserved sequence of the gene TP53, the primer for the mutation ARMS can be specifically combined with the corresponding mutation sequence, and the mutation sequence is amplified. The detection probe can be combined with amplified fragments, the blocking probe can be specifically combined with a wild type sequence corresponding to a mutation site, and wild type non-specificity amplification is restrained. The specificity mutation primers and the probe blocking technology are adopted, and the five mutation types of the gene TP53 can be accurately detected; mutations of the gene TP53 are rapidly detected conveniently through the built reagent kits of a real-time fluorescence PCR amplified reaction system, operation is easy, and the result is easy to read. The detection method is high in sensitivity, and 50-copy mutation can be stably detected; the specificity is good, 20 ng wild type genomic DNA non-specificity amplification is achieved, and the detectability reaches up to 1%.

The invention provides a primer for identifying coilia ectenes and coilia brachygnathus and a molecular biological method. The primer contains an amplified primer group and an extended primer group. A nucleotide sequence of the amplified primer group is shown as SEQ ID NO:1-16, a nucleotide sequence of the extended primer group is shown as SEQ ID NO:17-24. The primer combination can be effectively used for identifying the coilia ectenes and the coilia brachygnathus. The molecular biological method comprises the steps that a to-be-tested coilia ectenes and coilia brachygnathus SNP locus area nucleic acid sequencing library is established, sequencing is conducted on the library to obtain a sequencing result; based on the sequencing result, a coilia ectenes and coilia brachygnathus SNP locus area nucleic acid sequence is determined; then Structure2.3.2 software is utilized to draw a column diagram of population genetic differences and a column diagram of individual genetic differences, and species are judged by utilizing analysis. The method includes simple and convenient steps and is accurate, efficient and low in cost.

A recording apparatus is provided. The recording apparatus is a recording apparatus having a scanning function, and is excellent in operability when reading a document the size of which is larger than the size of a document reading area. A printer includes a scanner unit which reads a document; and a recording portion which records information read by the scanner unit. In the recording portion, the maximum recordable size of a paper sheet is greater than a size of a document reading area (30a) in the scanner unit. At a base end side of a cover (4) which opens and closes the document reading area (30a), a gap (35) which passes the document which is pushed out from the document reading area (30a) and of which the size is greater than the size of the document reading area (30a) to an outer side of the scanner unit is formed.

The invention discloses a multi-target microarray chip full-automatic hybrid cleaning dyeing system comprising a multi-target microarray chip card box and a full-automatic hybrid cleaning dyeing system. The automatic hybrid cleaning dyeing system comprises a sample and reagent processing module, an incubation reaction module and a fluid control module. The disposable visual multi-target microarray chip card box adopts a fixed closed system, so that the risk of pollution is reduced, results are convenient to read, and fluid operation is convenient in a process that an instrument automatically performs a reaction; and the full-automatic hybrid cleaning dyeing system accelerates the experimental speed, and improves repeatability and consistency of experimental results.

The invention discloses a visual micro-fluidic chip for multi-virus nucleic acid detection, and a detection method of the visual micro-fluidic chip. An extraction layer, a separation layer and an amplification detection layer are arranged from top to bottom in sequence; the extraction layer is used for distributing a sample solution into a plurality of reaction cavities; the separation layer is used for separating the extraction layer from the amplification detection layer; and the amplification detection layer is used for carrying out amplification detection on different viral nucleic acids. The method is used for solving the problem that nucleic acid cannot be efficiently, accurately, simply and rapidly detected by aiming at POCT requirements.

The invention provides a using method of a follicle-stimulating hormone colloidal gold assay kit. The method comprises the following steps of: 1, taking out a test card, a colloidal gold marker and cleaning liquid from a kit; 2, laying the test card on a tabletop, dripping 1 to 2 drops of a urine sample to be detected in a feeding hole, and standing until the urine sample is completely permeated; 3, dripping 1 to 2 drops of colloidal gold reagent into the feeding hole, and standing until the reagent is completely permeated; and 4, dripping 2 to 3 drops of the cleaning liquid into the feeding hole, and standing until the cleaning liquid is completely permeated. A positive reaction can be determined when a quality control hole and a detection hole simultaneously display clear light red or red spots, and the positive degree is correspondingly prompted according to the shade of the spots; or a negative reaction can be determined when only the quality control hole displays clear light red or red spots.