Histoplasma capsulatum infectious molecular diagnosis reagent kit based on recombinase and polymerase amplification technological principle and application thereof

Abstract

The invention relates to a histoplasma capsulatum infectious molecular diagnosis reagent kit based on the recombinase and polymerase amplification technological principle and application thereof. A primer is designed for an M antigen gene of an rDNA area of a histoplasma capsulatum capsule variation distributed according to the most common advantages of histoplasma capsulatum in clinical in China, the primer reacts with DNA of 30 histoplasma capsulatum standard strains, clinic and environment separated strains and 50 strains in relevant species according to an RPA system, and it is proved that the primer has excellent specificity. It is also proved that the primer has very high sensitivity through sensitivity testing. Accordingly, the primer can be used for early clinical diagnosis of histoplasma capsulatum infection, the problems that in clinical, histoplasma capsulatum infection confirmed diagnosis period is long and requirements for detection staff skills and laboratory platform conditions are high are solved, the primer can be prepared into the reagent kit, and convenience is provided for early diagnosis and timely treatment of clinical histoplasma capsulatum infection.

Description

technical field [0001] The invention relates to the technical field of molecular detection, in particular to a molecular diagnostic kit for early diagnosis of histoplasma infection based on the principle of recombinase polymerase amplification technology and its application. Background technique [0002] Histoplasma is a biphasic fungal genus commonly found in bird and bat feces, in its natural state a mycelial phase that transforms into a yeast phase in animals or humans. The genus Histoplasma comprises three species, namely Histoplasma capsulatum, Histoplasma capsulatum var. duboisii, and Histoplasma capsulatum var. farciminosum. Among them, Histoplasma capsulatus mainly infects animals, causing lymphangitis and epizootics in animals such as horses; Histoplasmosis (Histoplasmosis, HP) is caused by biphasic Histoplasma It is a fungal disease widely distributed in the world, which is mainly divided into American HP caused by H. capsulata and African HP caused by H. capsulat...

AI Technical Summary

Problems solved by technology

[0006] In summary, based on the sequence analysis of each DNA fragment of the histoplasma strain genome, the appropriate target gene was screened out, and a technical method for the early and rapid diagnosis of histoplasma infection based on the principle of RPA technology was developed, which is very important for solving clinical problems. The long time period for diagnosis of upper histoplasma infection and the high requirements for testing personnel skills and laboratory platform conditions are of great significance, which will provide convenience for early diagnosis and timely treatment in clinical practice

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