472 results about "Molecular diagnostics" patented technology

The invention belongs to the technical field of organic synthesis, and particularly discloses an ammonia aromatic alkene modified near-infrared BODIPY fluorescent probe as well as a preparation method and application thereof.The near-infrared BODIPY fluorescent probe is obtained by adopting 6-(dimethylamino) nicotinic aldehyde to directionally modify the alpha position of BODIPY and constructing an expanded conjugated system through Knoevenagel condensation. Wherein dimethylamino provides a strong electron donating effect, a molecular structure is twisted into a non-planar configuration due to steric hindrance of a pyridine ring, pi-pi accumulation is reduced, and an ACQ effect is inhibited. According to the structural modification strategy, significant red shift of absorption / emission wavelength is realized by expanding a molecular pi-conjugated skeleton, and meanwhile, the stability of the BODIPY core and the quantum yield are effectively enhanced. The probe molecule can specifically recognize the diphenylalanine dipeptide structural unit by utilizing the synergistic coordination effect of the 2-aminopyridine derivative and the BODIPY mother nucleus, so that the selective detection of the Alzheimer's disease biomarker Abeta42 is realized, and a novel optical detection tool is provided for the early molecular diagnosis and targeted therapy of AD (Alzheimer's disease).

This disclosure relates to apparatus and methods for molecular diagnostics. Certain embodiments include a piston cycled from a first position proximal to a first end of a housing, to a second position proximal to a second end of the housing, and back to the first position proximal to the first end of the housing. In some embodiments, the present disclosure relates to devices, methods, and systems for molecular diagnostics that do not comprise a piston.

The present invention relates to the technical field of medical instruments, and relates to the technical field of small amplification devices, especially portable amplification devices. Disclosed are a pressing and sealing apparatus for a POCT kit, and a molecular diagnostic device and operation method. The pressing and sealing apparatus for a POCT kit comprises a movable bracket, wherein a transmission assembly can drive the movable bracket to enable a sealing portion to seal a reaction chamber of the POCT kit when the movable bracket moves in a first direction; and when the transmission assembly drives the movable bracket to move in a second direction, a pressing portion comes into contact with the reaction chamber of the POCT kit to enable the reaction chamber of the POCT kit to tightly fit with a heat source. In the molecular diagnostic device including the pressing and sealing apparatus, a valve control portion and a clamping device share a second drive motor, a hot-melt bonding portion of the sealing portion and a detection and acquisition unit of the pressing portion share a first drive motor, a gas source drive part and a clamping apparatus cooperate with each other to drive liquid to flow through chambers of the kit, thereby achieving high integration, few drive parts, reliable operation, high detection accuracy, and small contamination.

The invention discloses a systemic lupus erythematosus molecular diagnosis marker based on neutrophil characteristics. The invention provides application of a substance for detecting the expression quantity of an IFIH1 gene and / or a CFLAR gene in preparation of a product for auxiliary diagnosis or screening or auxiliary screening of systemic lupus erythematosus. The invention also provides an application of a substance for detecting the relative expression quantity of the IFIH1 gene and the CFLAR gene and a carrier loaded with the following combined index C value model formula in preparation of a product for auxiliary diagnosis or screening or auxiliary screening of systemic lupus erythematosus. The specific gene expression marker IFIH1 and / or CFLAR of the neutrophile granulocyte is selected by focusing the unique change of the SLE neutrophile granulocyte to assist the clinical early-stage identification and diagnosis of the SLE, and an IFIH1 and CFLAR combined diagnosis model is constructed, so that the diagnosis or screening of the SLE is realized; the kit has high sensitivity and specificity.

The invention belongs to the technical field of molecular biology, and particularly discloses heat-resistant MMLV reverse transcriptase and application thereof. According to the heat-resistant MMLV reverse transcriptase provided by the invention, T246K mutation is introduced into a DNA polymerase active structural domain of a wild type MMLV reverse transcriptase, so that the heat stability and reverse transcription activity of the enzyme are remarkably improved. E286R, E302K and / or L435R mutations are further introduced into a thumb domain and a connecting structural domain, and the heat resistance of the enzyme can be improved by cooperating with T246K mutations. Meanwhile, D524G, E562Q and / or D583N mutations are introduced into an RNase H active structural domain, so that the RNase H activity of the enzyme can be reduced or removed. Compared with a commercial enzyme, the heat-resistant MMLV reverse transcriptase has the optimal reaction temperature higher than 50 DEG C, is suitable for high-temperature reverse transcription reaction, and has wide application prospects and higher economic value in the fields of molecular diagnosis and medical examination.

The present invention relates to a method for the ex vivo molecular diagnosis of gastrointestinal stromal tumors, comprising the steps of: providing at least one isolated tissue sample comprising at least one nucleic acid and the subsequent extraction of the at least one nucleic acid. The method according to the present invention further comprises a step of quantifying the at least one previously isolated nucleic acid. Furthermore, the method according to the present invention comprises preparing at least one amplicon library of said previously isolated nucleic acid, which comprises sequences obtained from the FGFR1, NF1, SDHA, SDHB, SDHC and SDHD genes. This step is followed by the step of purifying the at least one amplicon library and the step of simultaneously sequencing the amplicons themselves. The present invention also relates to a multi-gene panel for ex vivo molecular diagnosis of gastrointestinal stromal tumors and a kit comprising such a panel.

The invention relates to the technical field of crossing of biomedical engineering, molecular diagnosis and nano-optical sensing, and particularly discloses a virus nucleic acid variation amplification-free LSPR (Local Surface Plasmon Resonance) detection method and chip based on a gold nano-structure. The virus nucleic acid variation amplification-free LSPR detection chip based on the gold nanostructure comprises a sample injection port, a reaction area, a washing area, a detection area and a waste liquid area, the sample injection port is used for injecting a viral nucleic acid or protein sample, a plurality of groups of probe sequences are fixed in different reaction regions of the chip, non-amplification rapid detection of viral nucleic acid variation is realized, high sensitivity and specificity are ensured, and by utilizing the unique advantages of an MSNs template branched gold nanostructure, the detection sensitivity is improved. Refractive index change caused by single base mutation is directly amplified, the capability of completing femtomole-level detection within 30 minutes is achieved, the overall detection speed is increased, rapid typing detection of viral nucleic acid variation is achieved through LSPR spectral signals under the non-amplification condition, and a portable platform is formed by integrating the LSPR spectral signals and a micro-fluidic chip.

The invention relates to the technical field of biosensors, in particular to a novel thin film material for a molecular diagnosis nascent biosensor, a thin film transistor and a preparation method and application of the novel thin film material and the thin film transistor. The thin film transistor is prepared from a novel thin film material, the thin film material covers the surface of a substrate of the transistor, and a source electrode and a drain electrode of the transistor are arranged between the substrate and a thin film in parallel. The concentration of the cancer marker is detected by monitoring the variable quantity of threshold voltage (Vth) in a transfer curve of the organic-inorganic hybrid thin film transistor, the responsivity and sensitivity to a disease marker are improved by optimizing a semiconductor material combination, efficient detection of the low-concentration cancer marker is realized, and the method has the characteristics of no label, rapidness, simplicity and convenience, and is suitable for popularization and application. The kit is suitable for early diagnosis of diseases.

The invention belongs to the field of nucleic acid detection and molecular biology, and particularly relates to a method and a kit for eliminating false positive results in nucleic acid amplification reaction. According to the method, after a nucleic acid amplification reaction (taking recombinase polymerase amplification, namely RPA, for example) is completed, a target amplification product is subjected to selective enzyme digestion by utilizing restriction endonuclease, non-target products (such as primer dimers and non-specific amplification products) are not cut, and meanwhile, the non-target products which are not subjected to enzyme digestion are removed by combining solid-phase separation, so that the target amplification product is obtained. Therefore, the false positive result is eliminated. The invention also discloses a kit containing the restriction enzyme. According to the method, the specificity and the signal-to-noise ratio of a nucleic acid amplification reaction, especially RPA, are remarkably improved, the cost is low, operation is easy, the method is compatible with an existing technical platform, the method is suitable for detection scenes such as clinical molecular diagnosis, environmental monitoring and food safety, and the problem of misjudgment caused by false positive signals is effectively avoided.

The invention discloses a nucleic acid detection consumable processing module, a detection system and a use method, and belongs to the field of medical examination and inspection instruments and molecular diagnosis and detection instruments, the technical key points are that the nucleic acid detection consumable processing module comprises a driving module, a temperature control and clamping integrated module, an L-shaped consumable lifting bracket and a flip module; and the driving module is used for driving the temperature control and clamping integrated module to clamp / loosen a detection chip of the consumable, driving the L-shaped consumable lifting bracket to ascend / descend and driving the flip module to rotate at the same time. The invention aims to provide the nucleic acid detection consumable processing module, the detection system and the use method, and the operation of sample processing and detection is simplified.

The application discloses an exosome small non-coding RNA molecule marker and application thereof, and belongs to the field of tumor liquid biopsy and molecular diagnosis. The exosome small non-coding RNA molecule marker is at least one of tRNA-GlyGCC-5 and sRESE; the nucleotide sequence of the tRNA-GlyGCC-5 is shown as SEQ ID NO:1; and the nucleotide sequence of the sRESE is shown as SEQ ID NO:2. The sRESE in the application is a brand new small non-coding RNA sequence, and is first discovered by the inventor, which is important for the marker of esophageal cancer and the research on disease progression mechanism. The tumor diagnosis kit provided by the application, which comprises the exosome small non-coding RNA molecule marker, is a liquid diagnosis monitoring kit with non-invasiveness, high sensitivity, high specificity and applicability to general survey or screening.

A molecular diagnostic test device can include a housing and a sample preparation module within the housing. The sample preparation module defines an input reservoir and a sample input opening through which a biological sample can be loaded into the input reservoir. The sample preparation module includes a solid reagent contained within the input reservoir. The molecular diagnostic test device can include a fluid source within the housing. The fluid source is in fluid communication with the input reservoir such that upon activation of the molecular diagnostic test device, the fluid from the fluid source is injected into the input reservoir to mix the biological sample and the solid reagent within the input reservoir to form an input solution containing a target molecule.

The invention discloses a rapid culture system of mycoplasma pneumoniae. The rapid culture system comprises a growth-promoting additive A, a growth-promoting additive B and a growth-promoting additive C, wherein the growth-promoting additive A is A549 cell lysis buffer; the growth promoting additive B is a mixture of deoxyribonucleoside triphosphate and / or ribonucleoside triphosphate. The rapid culture system can promote rapid growth and reproduction of the mycoplasma pneumoniae, fully meets the nutritional requirements and growth requirements of the mycoplasma pneumoniae, and is of great significance to isolated culture, enrichment culture, epidemic disease prevention and control and molecular diagnosis of the mycoplasma pneumoniae. The invention also provides a culture method of the mycoplasma pneumoniae. The invention also provides application of the rapid culture system of the mycoplasma pneumoniae in in-vitro cell culture of the mycoplasma pneumoniae.

The invention relates to the technical field of molecular diagnosis, and discloses a miRNA combination for assisting hepatic fibrosis staged screening, a kit and a use method, the miRNA combination comprises miR-216b-5p, miR-2681-5p, miR-1178-3p, miR-1323 and miR-4418, the kit is used for ddPCR detection of the content of miRNA markers in a sample, and the kit comprises a miRNA marker reverse transcription primer and a miRNA marker amplification primer. The miRNA composition provided by the invention is an ideal and reliable miRNA marker used for assisting in hepatic fibrosis staged screening, the reverse transcription primer and the amplification primer provided by the detection kit have good specificity, high sensitivity and strong specificity, and a more accurate and reliable miRNA marker concentration value can be obtained based on the ddPCR technology in combination with the kit provided by the invention.

The invention discloses a cryptosporidium infection early warning method based on pasture environment and oocyst detection rate analysis, which comprises the following steps: collecting pasture multi-medium environment samples and corresponding parameters, and acquiring oocyst concentration by adopting a real-time quantitative PCR (Polymerase Chain Reaction) and a molecular diagnosis model; analyzing association between the oocysts and environmental factors through an oocyst propagation and environmental dynamics model; a key environment variable weight is determined by using MaxEnt and GloWPa-Crypto algorithms, and an oocyst diffusion path is simulated; and fusing multi-dimensional data to divide risk levels, and outputting the infection risk value and duration of each region through iterative operation. According to the method, through multi-medium sampling, multi-model fusion and multi-data deep fusion, the defects of single data, inaccurate propagation process description and insufficient data fusion in the prior art are overcome, the actual infection risks of different areas of the pasture can be accurately reflected, and a precise basis is provided for prevention and control.

The invention belongs to the technical field of tumor molecular diagnosis and biological information analysis, and relates to a high-throughput sequencing method and system for monitoring acute lymphocytic leukemia (MRD). Through targeted sequencing with a unique molecular identifier and / or a double-chain tag, error modeling based on a background noise spectrum and statistics / machine learning pseudo variation filtering, ultra-deep accurate detection of IG / TCR cloning and related gene low-frequency variation is realized. And an artificial intelligence recurrence risk prediction model is established by combining a time sequence MRD index, cloning diversity and clinical information, and a structured clinical report is output and docked with LIS / HIS. According to the method, the sensitivity and the specificity of ALL minimal residual disease detection can be remarkably improved, dynamic evaluation on leukemia cloning evolution and recurrence risks is realized, and a reliable basis is provided for individualized treatment decision and long-term follow-up visit.

The disclosure provides compositions, methods, and systems for implementation of high-performance molecular diagnostic assays involving color combinatorics, stimulus-responsive probes, tandem probes, conjugated polymer probes, and other mechanisms for increasing the number of targets that can be simultaneously detected in a digital assay. Multiplexed detection of targets is achieved in a rapid manner, with respect to sample partitioning and target detection using multiple color channels for detection.

The utility model discloses a fluorescence detection module, a cyclic amplification detection module and a small molecular diagnostic device. The fluorescence detection module includes a composite driving mechanism, a mobile bracket, a detection module, a hot melt joint and a transceiver detection module. The composite driving mechanism can drive the mobile bracket to drive the detection module and the hot melt joint to alternately contact or stay away from the integrated detection consumables. When the hot melt joint contacts the integrated detection consumables, the hot melt joint seals the reaction chamber of the integrated detection consumables. The detection module is provided with a detection optical fiber. When the detection module conflicts with the integrated detection consumables, the reaction chamber is closely fitted between the external heat source and one end of the detection optical fiber. The other end of the detection optical fiber is connected to the transceiver detection module. The multi-channel transceiver unit in the transceiver detection module scans the second end of the detection optical fiber to obtain the real-time fluorescence result in the reaction chamber. The fluorescence detection module provided by the utility model realizes the reliable fixed constraint of the detection optical fiber, so that the detection result is accurate and reliable.

The invention belongs to the technical field of characterization of target analytes, and provides application of PANX2 protein and a mutant thereof as biopore protein. The bioporous protein is used for detecting one or more of metal ions, inorganic salts, amino acids, nucleotides, small molecule drugs, small molecule diagnostic agents, adenosine triphosphate and monosaccharides. After one or more amino acids of the wild type PANX2 protein are mutated into common amino acids except original amino acids, compared with the wild type PANX2 protein, the PANX2 protein mutant can be normally expressed and is stable in property, a current signal is obviously improved during detection, the up-down amplitude of the current signal is longer, and burrs are fewer.

The invention relates to the field of molecular diagnosis and nucleic acid amplification, in particular to a recombinase polymerization amplification method based on Twinkle helicase enhancement and application of the recombinase polymerization amplification method. According to the invention, Twinkle helicase is introduced into an RPA system for the first time, and primer combination and template unlinking are assisted by utilizing ATP-dependent unlinking activity and DNA annealing activity of the Twinkle helicase, so that the amplification efficiency and the detection sensitivity are remarkably improved. The method is suitable for plasmid DNA, a high GC template and a clinical nucleic acid sample, and can be used for constructing an RPA kit containing Twinkle, so that the application of the RPA kit in pathogen detection, molecular diagnosis and on-site rapid detection is expanded. According to the invention, the defects of the existing RPA technology in sensitivity and stability are overcome, and a new solution is provided for developing a novel nucleic acid self-detection and field detection tool.

The present invention relates to the field of gene detection technology, and specifically discloses a molecular diagnosis pretreatment device and a molecular diagnosis pretreatment method, in which the opening mechanism includes a clamping assembly for clamping a cover body and a tube fixing assembly for fixing a sample tube, and the clamping end of the clamping assembly can rotate relative to the tube fixing assembly around the axis of the sample tube; the pipette in the cup-dividing mechanism is used to transfer the sample in the sample tube to multiple test tubes; the extraction mechanism includes a beating assembly and an extraction assembly, and the beating sleeve in the beating assembly is slidably arranged on the frame along the axial direction of the test tube, and can reciprocate in the test tube of the extraction station; the magnetic bar in the extraction assembly is slidably arranged on the frame along the axial direction of the test tube, and can enter the beating sleeve or move out of the beating sleeve, and the transmission mechanism is used to transfer the sample tube and the test tube. The above arrangement improves the extraction effect, is not easily contaminated, and improves work efficiency.

The disclosure provides compositions, methods, and systems for implementation of highly multiplexed molecular diagnostic assays involving color combinatorics, stimulus-responsive probes, tandem probes, conjugated polymer probes, and other mechanisms for increasing the number of targets that can be simultaneously detected in a digital assay. Multiplexed detection of targets is achieved in a rapid manner, with respect to sample partitioning and target detection using multiple color channels for detection. Implementation of methods described also achieve detection with significantly improved signal-to-noise ratio (SNR) values.

The utility model discloses a molecular diagnostic device, and relates to the field of diagnostic devices related to small-sized amplification equipment, especially portable amplification equipment. The molecular diagnostic device provided by the utility model can make the sample liquid or sample processing liquid added into the integrated detection consumable flow between the chambers of the integrated detection consumable through the operation part, so as to obtain the amplification system liquid located in the amplification chamber. The thermal circulation part can amplify and heat the amplification system liquid in the amplification chamber, and the detection part performs fluorescence detection on the amplification system liquid in the amplification to obtain the amplification detection result. The configuration of the amplification system liquid and the amplification detection can be completed through one device, and the integration is high, the number of driving parts is small, the operation is reliable, the detection accuracy is high, and the pollution is small.

The invention relates to the technical field of markers, in particular to application of a biomarker for identifying acute stage and chronic stage of brucellosis. A series of biomarkers related to acute and chronic stages of brucellosis are screened and found, and the biomarkers have obvious difference between acute and chronic brucellosis patients. The biomarkers can realize acute and chronic staging of brucellosis, are high in sensitivity and specificity, and have relatively good diagnosis efficiency. A novel molecular diagnosis tool is provided for acute and chronic staging of brucellosis, the problems that existing serological detection is low in specificity, and clinical staging cannot be accurately judged are solved, and wide application prospects are achieved.

A hand-held molecular diagnostic test device includes a housing, an amplification (or PCR) module, and a detection module. The amplification module is configured to receive an input sample, and defines a reaction volume. The amplification module includes a heater such that the amplification module can perform a polymerase chain reaction (PCR) on the input sample. The detection module is configured to receive an output from the amplification module and a reagent formulated to produce a signal that indicates a presence of a target amplicon within the input sample. The amplification module and the detection module are integrated within the housing.

The invention discloses a plasma protein-based stomach cancer early-stage multi-molecular diagnosis model construction method, and aims to solve the problems that the existing stomach cancer early-stage diagnosis standard and minimally invasive or non-invasive method are imperfect, the sensitivity and specificity of the existing diagnosis molecular marker are low and the like, an Olink proteomics PEA technology is utilized and a specific detection panel is selected to construct a stomach cancer early-stage multi-molecular diagnosis model. And identifying the protein markers of gastric cancer, early gastric cancer and gastric precancerous lesions in a discovery queue (n = 88). In a discovery queue, a group of multi-protein molecular marker models consisting of 13 proteins are successfully identified by utilizing an LASSO regression model, and the multi-protein molecular marker models have good diagnostic performance for distinguishing gastric cancer, early gastric cancer and pregastric lesion and contrast in the discovery queue and verification pair. Furthermore, in a verification queue sample, the diagnosis model also shows good diagnosis performance, and the marker model is expected to become a powerful tool for early diagnosis of gastric cancer.

The invention relates to the field of molecular diagnosis and tumor risk assessment, in particular to a marker combination and a kit for assessing the lymphatic metastasis risk of cervical cancer and application of the marker combination and the kit. The marker comprises a group of gene markers and a group of microbial markers, the gene markers comprise CARD9, MNX1AS2, MRAS, OLFML2A and RPS28, preferably, the gene markers further comprise at least one of CFL1P1 and GRASLND, and the microbial markers comprise microorganisms of the genus Dialister, the genus Catonella and the genus Campylobacter. The marker provided by the invention can be used for constructing an accurate risk assessment model, and can be used for developing a corresponding PCR (Polymerase Chain Reaction) kit, a mass spectrum or a chromatographic analysis system to realize metastasis risk prediction, curative effect assessment and new drug screening of cervical cancer patients.

The invention discloses a multiple qPCR (quantitative polymerase chain reaction) detection reagent for detecting neonatal gastrointestinal infection pathogens. The kit comprises specific primers and probes for detecting enterohemorrhagic escherichia coli, staphylococcus aureus, pseudomonas aeruginosa, streptococcus agalactiae, klebsiella pneumoniae, staphylococcus epidermidis, beta hemolytic streptococcus, salmonella, rotavirus and adenovirus. According to the invention, different fluorescently-labeled probes are utilized to add specific primers and probes of multiple pathogens into a reaction system to detect multiple targets at the same time, and the method has the advantages of good specificity, high sensitivity, short detection period, capability of simultaneously detecting multiple pathogens, rapidness, simplicity, convenience and economy; a convenient method is provided for rapid detection of pathogens causing neonatal gastrointestinal infection, and the kit has important significance in epidemiological research, clinical early molecular diagnosis and the like.

The present invention relates to a probe set for an isothermal single reaction using a split T7 promoter and a use thereof. In the present invention, a design is made to introduce a split T7 promoter into a 3-way junction structure to produce a large quantity of fluorescent RNA aptamers at an isothermal temperature in the presence of a target molecule, and a nucleic acid biomarker can be detected rapidly and conveniently with only one enzyme in one pot. The probe set allows for various applications through high-sensitivity detection of multiple nucleic acid biomarkers and detection in which analysis is performed without an additional nucleic acid extraction process. In one pot, multiplex analysis can be conducted and molecular diagnosis can be made of various entities including viruses and pathogens.

The invention relates to a unified system for the extraction and purification of biomolecules using a novel Cloudy-Precipitated (CP) Buffer and an integrated extraction device. The CP Solution remains cloudy even when heated and enables single- or multi-step workflows for isolating nucleic acids without requiring multiple buffers or organic solvents. It contains optimized concentrations of detergents, chaotropic agents, salts, and adsorbents to efficiently lyse cells and minimize processing steps. The associated device incorporates a heating and filtration system that allows separation of target molecules from inhibitors or impurities based on molecular weight. It can operate as a single- or double-step configuration with various filter membrane options. This system reduces time, labor, and environmental impact while enhancing sample quality for molecular diagnostics, research, and therapeutic applications.