Molecular diagnosis micro-fluidic chip, molecular diagnosis micro-fluidic chip system and applications of molecular diagnosis micro-fluidic chip and molecular diagnosis micro-fluidic chip system

Abstract

The invention relates to the field of micro-fluidic chip molecular diagnosis, and in particular to a molecular diagnosis micro-fluidic chip, a molecular diagnosis micro-fluidic chip system and applications of the molecular diagnosis micro-fluidic chip and the molecular diagnosis micro-fluidic chip system. The chip comprises a sample injection unit (1) which is used for receiving to-be-detected samples, at least one liquid storage unit (2) for storing reaction reagents, a cell lysis unit (3) for conducting cell lysis and a nucleic acid amplification unit (4) for conducting nucleic acid amplification, wherein the cell lysis unit comprises a slit (31) as well as a first compartment (32) and a second compartment (33) which are separated by virtue of the slit; at least one compartment is configured with grinding micro-beads; the first compartment communicates with the sample injection unit; and the second compartment communicates with the at least one liquid storage unit, so as to receive acell lysis solution. The chip system is composed of three layers, namely an upper layer, a middle layer and a lower layer, wherein the chip is arranged on the middle layer; the upper and lower layersare used for covering and sealing the middle layer; the upper layer is provided with a sample injection hole which is connected to the sample injection unit, and the sample injection hole correspondsto an avoiding hole of the liquid storage unit. The chip is simple in detection process, high in sensitivity and strong in repeatability, and reaction samples can be quantified.

Description

technical field [0001] The invention relates to the field of microfluidic chip molecular diagnosis, in particular to a microfluidic chip for molecular diagnosis and a fluidic chip system for molecular diagnosis, and their application in nucleic acid amplification. Background technique [0002] Microfluidics, also known as microfluidic chip laboratory or chip laboratory (lab-on-a-chip), refers to a chemical or biological laboratory built on a chip of a few square centimeters. , it integrates the basic operation units involved in the fields of chemistry and biology, such as sample preparation, reaction, separation, detection, cell culture, sorting, lysis, etc. The control fluid runs through the entire system to realize various functions in the fields of biology, chemistry, and medical diagnosis. [0003] The basic characteristics and biggest advantages of microfluidic chip technology are: a variety of unit structures can be flexibly combined on the tiny chip platform, making ...

AI Technical Summary

Problems solved by technology

After patent research, it was found that there have been a small number of patents related to molecular diagnosis using microfluidic technology at home and abroad in recent years. It has the function of nucleic acid amplification, but does not have the functions of reagent storage, reagent mixing, and sample quantification; another example is a centrifugal disk array nucleic acid detection microfluidic chip disclosed in Chinese patent 201610242532, but the chip is only for nucleic acid extraction For detection with liquid, the user needs to manually process the sample to be tested to extract the nucleic acid in the sample before use, and then inject the nucleic acid into the chip for detection. The use of this chip has raised user requirements, and the nucleic acid needs to be extracted in advance

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