2536 results about "Microfluidics" patented technology

The invention relates to an optimized method for medicament preparation. A machine learning model of a deep neural network is adopted to perform screening and combinatorial optimization on active ingredients and auxiliary materials of the medicament, and a relation model among the ingredients, the drug effect and the stability is established; a dynamic blending module is adopted to adjust the component proportion and dosage in real time according to different medicament preparation conditions including temperature and pH value; constructing a self-adaptive reaction condition adjusting system, and monitoring reaction conditions including temperature, pressure, pH value and stirring speed in the preparation process in real time; a dynamic mixing system is introduced, and a microfluidic technology is adopted to control metering and mixing of pharmaceutical components; a medicament preparation process is decomposed into a plurality of key steps including primary reaction, purification and concentration, and each step is independently optimized; a real-time quality monitoring system is constructed, and the physical properties, including particle size distribution, solubility and purity, of the medicament are monitored in real time through a sensor and imaging equipment; and optimizing the integration platform in the whole process.

The invention relates to the technical field of gas monitoring, and discloses an air quality monitoring method based on an olfactory chip, which comprises the following steps: step 1, preparing a multi-modal sensor system which comprises an olfactory chip module, a surface enhanced Raman scattering module and a microfluidic time division multiplexing module, the sensor surface of the olfaction chip module is modified with a metal organic framework material, and the substrate of the surface enhanced Raman scattering module is made of a core-shell structure nano material; and 2, synchronously collecting resistance response data of the olfaction chip module, spectral data of the surface enhanced Raman scattering module and temperature, humidity and wind speed data of the environment sensor. According to the invention, a multi-modal sensor cooperative detection technical scheme is adopted, the high-sensitivity detection effect on low-concentration harmful gas in air is achieved, and compared with a technical scheme of a single sensor and a fixed fusion algorithm in the prior art, the defect that accurate recognition of trace gas cannot be realized under ppb-level concentration is overcome.

The invention provides a micro-flow field measurement method of a mechanical biological net port flowmeter, and belongs to the technical field of micro-flow field measurement, and the micro-flow field measurement method comprises the following steps: firstly, constructing a net port cavity digital model, and realizing micro-flow field high-resolution characterization by using fluorescent particle tracing liquid and a laser confocal system; and recording a fluid movement track and processing original data by applying a track optimization function under the condition that the microfluidic driving system generates a constant pressure gradient. And solving fluid pressure distribution by adopting an orthogonal grid system and a Navier-Stokes equation, and processing speed field and pressure distribution data by utilizing a pre-trained micro-flow field analysis neural network model. Finally, the flow and pressure relation curve is obtained by calculating the flow flux of the cross section of the network port, a micro-flow field measurement correction coefficient table is generated, and the technical problems that in the prior art, the accurate measurement difficulty of the biological network port micro-flow field is large, and multi-scale flow characteristic representation is insufficient are solved.

The invention provides a microfluidic sorting system and method, and relates to the technical field of particle sorting, and the microfluidic sorting system comprises a microfluidic sorting chip, a liquid driving device, an image acquisition device and a controller. According to the microfluidic sorting system and method provided by the invention, the required target particles can be sorted from the to-be-sorted particles on the basis of an image acquisition device and a deep learning technology under the condition that the to-be-sorted particles do not need to be subjected to fluorescent staining, so that the influence and damage to the to-be-sorted particles due to fluorescent staining can be reduced, and the sorting efficiency is improved. According to the method, the accuracy and credibility of subsequent experiments can be improved, the investment cost required by cell sorting can be remarkably reduced, multi-dimensional information such as cell size, roundness, edge characteristics and morphological characteristics can be obtained, the multi-dimensional cell sorting requirements can be better met, target particles can be more accurately and efficiently sorted, and the cell sorting efficiency is improved. The multi-dimensional cell sorting requirement can be better met, and flexible sorting and lossless sorting of cells can be achieved.

The invention provides an UTR element NHP1 as well as a construction method and application thereof, and relates to the technical field of mRNA. A 5 'UTR with a good expression effect is designed by integrating dominant sequences of a human high-expression gene and a pathogen natural UTR, a chimeric structure NHP1 with high ribosome load is predicted through a calculation model, a DNA sequence of the NHP1 is as shown in SEQ NO 1, and an RNA sequence of the NHP1 is as shown in SEQ NO 2; an EGFP report system is adopted on the DNA level to rapidly screen UTR; the translation efficiency is quantitatively evaluated on the RNA level through luciferase mRNA (N1-methyl pseudouridine modification); and the particle size is controlled by a microfluidic technology, so that the optimized UTR-mRNA is efficiently expressed after being delivered.

The invention discloses a capillary pressure and contact angle synchronous characterization method and device of a microfluidic fluid interface, and relates to the field of interface analysis and multiphase seepage in pore media, and the method comprises the following steps: based on a pre-configured pore, particle and fluid binary image, distinguishing and extracting a fluid-particle contact interface contour and an attack / defense fluid interface contour; fitting and determining the curvature radius of each fluid interface contour through a watershed region division algorithm and reordering smoothing of Fourier descriptors; generating a multi-level size pore space based on the pore binary image, and obtaining a pore overlook radius; calculating a necking angle in combination with the curvature radius and the multi-stage size pore space to obtain an apparent contact angle and an intrinsic contact angle; and calculating the capillary pressure of the attack / defense fluid interface by combining the curvature radius, the pore overlook radius, the chip depth and the interfacial tension. According to the invention, high-precision technical support is provided for micro-fluidic multiphase seepage research.

The invention discloses a shale oil high-pressure imbibition micro-fluidic experimental device and method, the shale oil high-pressure imbibition micro-fluidic experimental device comprises a nano-fluidic chip holder, the nano-fluidic chip holder is provided with a matrix type chip model, and the nano-fluidic chip holder is connected with a water bath box; the matrix type chip model is communicated with a pressure tracking pump and a vacuum pump, and the communicating position of the pressure tracking pump and the vacuum pump is communicated with an intermediate container and a power pump in sequence. And a full-period production dynamic process of fracturing fluid injection, well closing and well opening oil extraction under the shale reservoir condition is simulated by utilizing a nanofluidic technology. Meanwhile, a microfluidic experimental method is established by utilizing the experimental device, the flowing process and the distribution state of the oil-water two phases in the whole process of pressing, stuffiness and production are visually monitored and recorded in real time, and the imbibition replacement efficiency is quantitatively calculated by utilizing an image processing method.

The invention provides a micro-fluidic device and a preparation method thereof. The micro-fluidic device comprises a micro-fluidic substrate and a control substrate, the microfluidic substrate comprises a first substrate and a plurality of electrode pairs; the control substrate comprises a second substrate, a control circuit layer and a plurality of contact pairs; the first surface of the first substrate is provided with a plurality of opening areas, each opening area is provided with two accommodating grooves which are arranged at intervals, and the two accommodating grooves are communicated through a microfluidic channel on a common side wall; the plurality of electrode pairs are arranged on the first substrate and are arranged corresponding to the opening area. The control circuit layer is arranged on the second substrate; the plurality of contact pairs are arranged on the second substrate and are electrically connected with the control circuit layer; the control substrate is attached to the microfluidic substrate during use, the plurality of contact pairs are electrically connected with the plurality of electrode pairs, and the control circuit layer is used for applying voltage to each electrode pair. According to the microfluidic device, a plurality of samples are processed in parallel, the detection efficiency is improved, and the replacement cost can be reduced.

The utility model discloses a microfluidic system for target object pairing, which comprises a first flow channel, a second flow channel, an oil liquid flow channel, a buffer liquid flow channel, a matching flow channel and a pairing micro valve, a first micro valve, a second micro valve and a buffer liquid micro valve are arranged, the matching flow channel is communicated to the oil liquid flow channel, and the first flow channel, the second flow channel and the buffer liquid flow channel are all communicated to the matching flow channel; when a single first target object and a single second target object exist in the matching runner, the first micro valve and the second micro valve are in a closed state, and the matching micro valve and the buffer solution micro valve are in an open state; and the buffer liquid in the buffer liquid flow channel feeds the single first target object and the single second target object in the flow channel into the oil liquid in the oil liquid flow channel to form liquid drops. According to the utility model, other first target objects and / or other second target objects can be completely prevented from entering liquid drops, so that the success rate of packaging is improved, and the cell loss caused by multiple packaging is reduced.

An on-chip mid-infrared micro-ring resonant cavity microfluidic spectrometer is integrated on a substrate material and comprises a spot size converter, an optical coupler, a microfluidic module, a micro-ring resonant cavity, a miniature thin-film resistance heater, a sequential control power supply module, an optical filtering system and a photoelectric detector array. Selective filtering is performed on an input optical signal, and the flow and flow velocity of liquid or gas to be detected are controlled by using the microfluidic module, so that selective absorption of spectral components of the liquid or gas to be detected is realized. In addition, the invention also discloses a mid-infrared integrated optical chip micro-fluidic multiphase detection device comprising the spectrometer and a detection method thereof, the device also comprises a pumping and reference light source module and an on-chip mid-infrared frequency comb generation module, a broadband frequency comb is generated as a light source, a photoelectric detector array is used for collecting signals, and the detection precision is improved. The detection and inversion of the type and concentration of multi-component liquid or gas to be detected are realized, and the on-chip, real-time and micro detection of multi-component substances is realized.

The invention relates to the technical field of preparation of a graphene lubricating oil additive through a graphene uniform dispersion technology, and discloses a preparation method of an efficient dispersion type graphene composite lubricating oil additive, which comprises the following steps: a) carrying out surface modification on graphene by utilizing plasma treatment; b) mixing the modified graphene with a surfactant; c) uniformly mixing the graphene dispersion liquid with poly-alpha-olefin (PAO) base oil with specific molecular weight distribution on a nanoscale by adopting a microfluidic technology to form a premix; d) adding a custom-formulated antioxidant, an extreme pressure additive and nanoscale silicon dioxide particles and / or metal oxide into the premix; through plasma treatment and ultrasonic treatment conditions, the compatibility of graphene and base oil is remarkably enhanced, the comprehensive performance of the lubricating oil is improved, meanwhile, the antioxidant, the extreme pressure additive and the nano material are introduced, and the wear resistance, oxidation resistance and extreme pressure performance of the composite additive are remarkably improved.

The utility model relates to the field of microfluidic detection and fluorescence immunoassay, in particular to a portable biochemical blood coagulation and fluorescence immunoassay integrated analysis device, which comprises a shell, a power supply arranged in the shell, two support plates arranged in the shell, a fluorescent bracket fixedly connected to the upper ends of the support plates, a support arranged in the shell, a lamp source arranged at the upper end of the support, and a light source arranged at the lower end of the lamp source. A microfluidic detection module is mounted in the support, a fluorescence immunodetection module is mounted at the upper end of the fluorescence bracket, an optical module is arranged in the fluorescence immunodetection module, and a bar code scanner for confirming information of a detection chip or a reagent strip is arranged on one side of the optical module; compared with the traditional detection equipment with a single function, the double detection functions of biochemistry and fluorescence immunity are realized, the detection efficiency and comprehensiveness are greatly improved, meanwhile, the kit also supports the simultaneous detection of three reagent strips with different specifications, and the flexibility and convenience of the detection are further enhanced.

The invention relates to a wearable aptamer electrochemical sensor and a preparation method and application thereof, and belongs to the field of wearable sensors, and the preparation method comprises the following steps: 1) preparing an electrochemical sensing unit on a flexible substrate, including a TNF-alpha working electrode, a sodium ion working electrode and a pH working electrode; 2) preparing a microfluidic layer with a sweat detection channel; and 3) combining the flexible substrate with the microfluidic layer, and correspondingly fitting the electrochemical sensing unit with the sweat detection channel so as to monitor TNF-alpha, sodium ions and pH in sweat. By adopting the overall flexible design, the sweat TNF-alpha monitoring device is suitable for noninvasive and dynamic monitoring of human body inflammatory factors in a resting state, and the accuracy of sweat TNF-alpha monitoring can be improved; the non-invasive monitoring problem of high sensitivity, high specificity, high accuracy and high stability of sweat inflammatory factors in a non-motion state is solved, and the prepared electrochemical sensor can be attached to the surface of human skin and can be further applied to monitoring and management of inflammatory diseases and related fields.

The invention discloses a biological in-vitro sample aging quality control detection method, and relates to the technical field of biological samples, and the method specifically comprises the following steps: screening metabolic markers based on biological physiological characteristics, and constructing an in-vitro quality attenuation model; collecting and preprocessing sample data; detecting the concentration of the metabolic marker through detection equipment; inputting marker concentration data into the model for dynamic comparison; and outputting aging classification, and guiding experiment scheduling operation. According to the method, effective use time efficiency is adopted for rapid judgment, the problem that experimental data is influenced by metabolic degradation of a sample after in-vitro is solved, time efficiency grading can be completed in a short time through multi-marker dynamic comparison and a machine learning model, the method is remarkably superior to a traditional method, and by combining a micro-fluidic detection device and an LSTM model, the detection accuracy is greatly improved. According to the method, the samples can be scheduled to proper experiment operation according to the effective time limit of the samples, waste of the samples is avoided, and after failure samples are detected, a user can be reminded to collect the samples again, so that the experiment process is prevented from being delayed.

This application discloses a rapid detection method for screening of dwarfism in children, which belongs to the field of intelligent medical technology. It includes the following steps: Step 1, accurately sampling through capillary effect, and automatically diluting the sample to ensure accurate concentration; Step 2, introducing the collected sample into a multi-channel microfluidic detection chip to achieve parallel quantitative detection of endocrine hormones; Step 3, real-time acquisition and amplification of weak biomolecule recognition signals to effectively improve the signal-to-noise ratio; Step 4, multi-dimensional analysis of the test results to achieve quantitative evaluation of growth and development, body mass index and sexual development indicators and risk prediction of endocrine diseases; Step 5, automatically integrating the analysis results and generating a standardized test report; Step 6, establishing a quality control mechanism throughout the entire detection process, including real-time monitoring of sample quality, tracking of detection process parameters, result consistency evaluation, and full traceability of detection data.

The invention relates to a non-linear seepage experiment method for gas breaking through a cover layer based on dark field imaging. The method comprises the following steps: placing a microfluidic rock chip on an objective table of a dark field scattering microscope; the injection end of the microfluidic rock chip is connected with a fluid injection module, and the outlet end of the microfluidic rock chip is connected with a downstream outflow pipeline; injecting the formation water solution into the micro-fluidic rock chip until the micro-fluidic rock chip is filled with the formation water solution; opening a gas injection system, and adjusting the dark field scattering microscope until a clear image of a micro-fluidic rock chip pore structure and carbon dioxide displacement formation water is obtained; injecting carbon dioxide with different pressures into the micro-fluidic rock chip, and observing nonlinear seepage migration images of carbon dioxide and formation water in the micro-fluidic rock chip; when it is observed that carbon dioxide migrates to the outlet end of the rock chip, the breakthrough moment is obtained, and the upstream and downstream pressure difference value of the microfluidic rock chip is the breakthrough pressure. According to the method, the nonlinear seepage of the carbon dioxide displacement formation water in the nanopores can be accurately observed, and the breakthrough pressure can be determined.

The invention relates to the technical field of data center thermal management, and discloses a data center liquid cooling precise temperature control method and system, and the method comprises the following steps: pre-analyzing a calculation task to generate a power consumption prediction sequence; combining the power consumption prediction sequence with the real-time state information of the liquid cooling system, calculating the heat cost of the task at each candidate calculation node through a digital twin model, and selecting a target execution node according to the heat cost; inputting the power consumption prediction sequence and real-time state information into a reinforcement learning controller, and generating a composite coordination action containing a flow regulation instruction and a dynamic power consumption upper limit instruction; and cooperatively controlling the micro-fluidic execution array and the chip power consumption management unit on the target execution node according to the composite coordination action. Through prospective power consumption prediction and two-way cooperative control from the heating end and the heat dissipation end, intelligent distribution and accurate temperature control of thermal loads are achieved, temperature overshoot is effectively restrained, and the overall operation energy consumption of the data center is reduced while the system reliability is guaranteed.

The invention discloses a method for preparing high-purity uranium carbide microspheres by a microfluidic-assisted internal gelation process, which comprises the following steps of: designing a uranium glue solution component containing carbon black to obtain a uranium glue solution which is stable for a long time at room temperature and quickly gelates at high temperature; the preparation method comprises the following steps: preparing gel microspheres with uniform size by using a uranium glue solution as a micro-fluidic dispersion phase through a micro-fluidic assisted inner gel process; the pore structure in the gel microspheres is maintained through washing and soaking with propylene glycol monomethyl ether, so that the crack-free gel microspheres are obtained; the UC nuclear fuel microspheres which are uniform in size, good in sphericity degree and high in phase purity are prepared through the carbon thermal reduction reaction of the non-cracking gel microspheres under the optimized sintering schedule, and development of ultrahigh-temperature gas cooled reactor nuclear fuel is expected to be promoted.

The invention discloses a micro-fluidic liquid cooling heat dissipation module integrated with piezoelectric driving, a chip and application, and belongs to the technical field of chip liquid cooling heat dissipation. The micro-fluidic liquid cooling heat dissipation module comprises a micro-fluidic substrate and a piezoelectric film, the piezoelectric film covers the surface of one side of the microfluidic substrate; a groove channel is formed in the surface of one side, facing the piezoelectric film, of the micro-fluidic substrate; a loop cavity defined by the piezoelectric film and the groove channel forms a micro-channel; a metal electrode layer, a heat source area and a heat exchange area are respectively arranged at different positions, corresponding to the micro-channel, on the surface of one side, deviating from the micro-fluidic substrate, of the piezoelectric film. The micro-fluidic liquid cooling heat dissipation module can actively drive the cooling liquid to flow to a heat source area, and compared with a traditional micro-channel heat dissipation mode, the micro-fluidic liquid cooling heat dissipation module has very high flexibility in fluid driving, does not need an external driving pump, can greatly reduce the size of a packaged chip, and is beneficial to development of chip integration and microminiaturization.

The technology described herein generally relates to microfluidic cartridges configured to amplify and detect polynucleotides extracted from multiple biological samples in parallel. The technology includes a microfluidic substrate, comprising: a plurality of sample lanes, wherein each of the plurality of sample lanes comprises a microfluidic network having, in fluid communication with one another: an inlet; a first valve and a second valve; a first channel leading from the inlet, via the first valve, to a reaction chamber; and a second channel leading from the reaction chamber, via the second valve, to a vent.

The invention provides a spiral structure inertia focusing microfluidic cell screening device and a screening method thereof.The spiral structure inertia focusing microfluidic cell screening device comprises a planar spiral structure, the planar spiral structure is a single-ring planar spiral structure, and a single-ring planar spiral structure channel in the planar spiral structure is provided with at least two inlet channels and at least three outlet channels; the single-ring plane spiral structure channel, the inlet channel and the outlet channel are rectangular cross section channels, the single-ring plane spiral structure is provided with a plurality of stabbing structures, and the injection pump is driven to adjust the flow of a blood sample and sheath flow liquid so as to adjust the starting point Reynolds number of the single-ring plane spiral structure channel, so that stable cell inertial focusing is realized; the device can replace a complex and high-cost multi-separation microfluidic device, is not easy to block, easy to clean, high in stability, not easy to deform, high in experiment accuracy and reliability, beneficial to long-term experiment operation and use, and high in repeated utilization rate.

The invention discloses an ultra-high-speed imaging device based on acousto-optic filtering modulation. The ultra-high-speed imaging device comprises a spectrum modulation system, a spectrum imaging system and a data processing system. The spectrum modulation system utilizes an acousto-optic tunable filter (AOTF) to dynamically modulate broadband continuous spectrum light, narrow-band illumination light which rapidly changes along with time is generated, and time information of a dynamic scene is effectively mapped to a spectrum dimension. And the spectral imaging system images the spectral coded dynamic scene to the hyperspectral camera to realize acquisition of spectral space-time information. The data processing system performs spectral crosstalk correction, channel separation and demosaicing reconstruction on the acquired original mosaic image, and recovers a time sequence image according to a spectrum-time mapping relation, thereby realizing single-shot superspeed imaging in a dynamic process. The device has the advantages of being simple and compact in structure, flexible in time window, high in imaging fidelity and the like, and has wide application prospects in the fields of rapid dynamics such as microfluidics, laser processing and ultrafast physics.

The invention discloses a craft IPA beer flora optimization method based on an improved bat algorithm. The method comprises the following steps: S1, constructing an initial state space of yeast flora proportion dynamic optimization; s2, standardized real-time process data are formed; s3, fusing the standardized real-time process data with the initial state space to obtain an updated comprehensive state vector, and dividing the whole fermentation process into a sugar collection stage, a main fermentation stage and an after-ripening stage according to a time sequence; s4, obtaining a staged entropy weight vector; s5, forming a multi-objective optimization problem; s6, obtaining an optimal flora proportion parameter set and an optimal fermentation regulation parameter set; and S7, transmitting the optimal flora proportion parameter set and the optimal fermentation regulation and control parameter set to a microfluidic bacterium throwing device, a material supplementing control unit and a frequency conversion temperature control unit to realize the optimization of the crafted IPA beer flora. According to the method, an optimization path can be effectively guided to be close to a flavor synergistic expression area, and the spatial consistency of yeast metabolism and aroma generation is greatly improved.

The invention discloses a sewage heavy metal ion detection device, which belongs to the technical field of sewage detection, and comprises a sensor module and a microfluidic module, the sensor module comprises a Loff wave surface acoustic wave device, the Loff wave surface acoustic wave device comprises a resonance area and two Loff wave interdigital transducers, the resonance area is located between the two Loff wave interdigital transducers, and the resonance area is covered with a heavy metal ion adsorption film; the micro-fluidic module comprises a micro-channel system and a Rayleigh wave surface acoustic wave device, the micro-channel system comprises a dropwise adding tank, a micro-channel and a waste liquid tank which are communicated in sequence, the micro-channel is provided with an experimental area overlapped with the resonance area, and the Rayleigh wave surface acoustic wave device is used for driving sewage to move from the dropwise adding tank to the waste liquid tank. By combining a microfluidic technology and a surface acoustic wave technology, heavy metal ions, especially lead ions, in sewage can be rapidly, accurately and conveniently monitored.

The invention relates to the field of pesticide composition screening, and discloses a stabilizer screening process for improving the low-temperature stability of a chlorantraniliprole and lufenuron suspending agent, which comprises the following steps: a) analyzing the low-temperature behavior of chlorantraniliprole and lufenuron based on molecular simulation, constructing a candidate stabilizer molecule library, and preliminarily screening candidate stabilizers; b) applying low-temperature stress to the chlorantraniliprole and lufenuron suspending agent sample containing the candidate stabilizer by using a microfluidic platform, and performing in-situ dynamic characterization to obtain dynamic data related to the low-temperature stability of the sample; and c) processing the dynamic data, and establishing an evaluation index representing the low-temperature stability performance of the candidate stabilizer. According to the invention, a cognitive bridge from a micromechanism to a mesoscopic phenomenon is constructed by integrating molecular simulation pre-screening and microfluidic high-throughput dynamic characterization. The problems of long research and development cycle and resource consumption caused by a large number of trial and error and experience judgment in a traditional screening method are effectively solved.

To provide methods for selectively combining discrete entities including cells, reagents, drugs, hydrogels, extracellular matrices, beads, particles, biological materials, media, or a combination thereof.SOLUTION: The method comprises sorting a plurality of discrete entities and trapping two or more discrete entities for a time sufficient for the two or more discrete entities to combine to form a combined discrete entity. In certain aspects, the method comprises making the plurality of discrete entities. In certain aspects, the method comprises detecting or analyzing the discrete entities, for example, via optical detection. In certain aspects, the method comprises manipulating or analyzing the combined discrete entity or a component therein, for example, imaging, sequencing, culturing, for example, three-dimensional culturing, and measuring cell-cell interactions.SELECTED DRAWING: None

The invention discloses a cortisol and sodium ion difunctional detection sensor and a preparation method thereof, and belongs to the technical field of sensing detection, the cortisol and sodium ion difunctional detection sensor comprises a substrate layer, a sodium ion detection module and a cortisol detection module, the bionic micro-fluidic unit is provided with a plurality of runner holes, and an embedded capillary pump structure is arranged in each runner hole so as to guide liquid to be detected to the sodium ion detection module and the cortisol detection module; and the shielding layer covers the surface of the bionic micro-fluidic unit. The sodium ion detection module and the cortisol detection module are specifically designed, so that wide-range detection of sodium ion concentration and low-concentration detection of cortisol are realized, a high-altitude low-pressure environment of a pilot can be matched, the sodium ion concentration and the cortisol concentration of the pilot can be accurately detected, and the detection accuracy is improved. Accurate detection of the signs of the pilot in the high-altitude flight process is achieved.

A microfluidic device includes a plurality of reaction wells; and a plurality of solid supports, and each of the solid supports has a reagent attached thereto. The reagent is attached to the solid support via a labile reagent / support bond such that the reagent is configured to be cleaved from the support via a cleaving operation.

The invention relates to the technical field of microfluidics, in particular to a high-throughput and high-efficiency cell extrusion microfluidic system and a particle introduction method. And a closed-loop control system integrating real-time cell deformation sensing, dynamic parameter decision and physical extrusion and alternating electric field cooperative application is constructed. The chip is provided with an inlet shunting device, a micro-channel and a channel structure of a microelectrode. The method comprises the steps of real-time deformation image acquisition and analysis and dynamic extrusion parameter decision and cooperative application. The invention provides a high-flux and high-efficiency cell extrusion microfluidic system and a particle introduction method, and aims to solve the problems that the drug loading efficiency and the survival rate are difficult to consider due to heterogeneity in the existing cell extrusion technology and a physical extrusion and electroporation coordinated regulation strategy is lacked, so that personalized precise treatment of cells is realized, and the cell quality is improved. The drug loading efficiency, the cell survival rate and the high throughput are obviously improved.

The invention relates to the technical field of oil exploitation, and discloses a surfactant-loaded microcapsule and a preparation method thereof. The preparation method of the microcapsule loaded with the surfactant comprises the following steps: respectively injecting an inner phase, an intermediate phase and an outer phase into a micro-fluidic chip for contact, and then separating to obtain the microcapsule, wherein the inner phase is an aqueous solution containing a surfactant I; the intermediate phase is a solution containing a hydrophobic material; the external phase is an aqueous solution containing a surfactant II; the inner phase and the outer phase are both insoluble with the intermediate phase; the flow velocity ratio of the inner phase to the middle phase is 1: (0.5-8); the flow velocity ratio of the inner phase to the outer phase is 1: (5-30); the concentration of the intermediate phase is 5-100 wt%. According to the microcapsule prepared by adopting a microfluidic technology, the adsorption of a surfactant in a near wellbore area is greatly reduced, so that the microcapsule fully plays an oil displacement role in a deep reservoir, and the recovery efficiency of an oil field is improved.