1540 results about "Microfluidics" patented technology

The invention provides an UTR element NHP1 as well as a construction method and application thereof, and relates to the technical field of mRNA. A 5 'UTR with a good expression effect is designed by integrating dominant sequences of a human high-expression gene and a pathogen natural UTR, a chimeric structure NHP1 with high ribosome load is predicted through a calculation model, a DNA sequence of the NHP1 is as shown in SEQ NO 1, and an RNA sequence of the NHP1 is as shown in SEQ NO 2; an EGFP report system is adopted on the DNA level to rapidly screen UTR; the translation efficiency is quantitatively evaluated on the RNA level through luciferase mRNA (N1-methyl pseudouridine modification); and the particle size is controlled by a microfluidic technology, so that the optimized UTR-mRNA is efficiently expressed after being delivered.

The invention discloses a capillary pressure and contact angle synchronous characterization method and device of a microfluidic fluid interface, and relates to the field of interface analysis and multiphase seepage in pore media, and the method comprises the following steps: based on a pre-configured pore, particle and fluid binary image, distinguishing and extracting a fluid-particle contact interface contour and an attack / defense fluid interface contour; fitting and determining the curvature radius of each fluid interface contour through a watershed region division algorithm and reordering smoothing of Fourier descriptors; generating a multi-level size pore space based on the pore binary image, and obtaining a pore overlook radius; calculating a necking angle in combination with the curvature radius and the multi-stage size pore space to obtain an apparent contact angle and an intrinsic contact angle; and calculating the capillary pressure of the attack / defense fluid interface by combining the curvature radius, the pore overlook radius, the chip depth and the interfacial tension. According to the invention, high-precision technical support is provided for micro-fluidic multiphase seepage research.

The invention provides a micro-fluidic device and a preparation method thereof. The micro-fluidic device comprises a micro-fluidic substrate and a control substrate, the microfluidic substrate comprises a first substrate and a plurality of electrode pairs; the control substrate comprises a second substrate, a control circuit layer and a plurality of contact pairs; the first surface of the first substrate is provided with a plurality of opening areas, each opening area is provided with two accommodating grooves which are arranged at intervals, and the two accommodating grooves are communicated through a microfluidic channel on a common side wall; the plurality of electrode pairs are arranged on the first substrate and are arranged corresponding to the opening area. The control circuit layer is arranged on the second substrate; the plurality of contact pairs are arranged on the second substrate and are electrically connected with the control circuit layer; the control substrate is attached to the microfluidic substrate during use, the plurality of contact pairs are electrically connected with the plurality of electrode pairs, and the control circuit layer is used for applying voltage to each electrode pair. According to the microfluidic device, a plurality of samples are processed in parallel, the detection efficiency is improved, and the replacement cost can be reduced.

The utility model relates to the field of microfluidic detection and fluorescence immunoassay, in particular to a portable biochemical blood coagulation and fluorescence immunoassay integrated analysis device, which comprises a shell, a power supply arranged in the shell, two support plates arranged in the shell, a fluorescent bracket fixedly connected to the upper ends of the support plates, a support arranged in the shell, a lamp source arranged at the upper end of the support, and a light source arranged at the lower end of the lamp source. A microfluidic detection module is mounted in the support, a fluorescence immunodetection module is mounted at the upper end of the fluorescence bracket, an optical module is arranged in the fluorescence immunodetection module, and a bar code scanner for confirming information of a detection chip or a reagent strip is arranged on one side of the optical module; compared with the traditional detection equipment with a single function, the double detection functions of biochemistry and fluorescence immunity are realized, the detection efficiency and comprehensiveness are greatly improved, meanwhile, the kit also supports the simultaneous detection of three reagent strips with different specifications, and the flexibility and convenience of the detection are further enhanced.

The invention discloses a biological in-vitro sample aging quality control detection method, and relates to the technical field of biological samples, and the method specifically comprises the following steps: screening metabolic markers based on biological physiological characteristics, and constructing an in-vitro quality attenuation model; collecting and preprocessing sample data; detecting the concentration of the metabolic marker through detection equipment; inputting marker concentration data into the model for dynamic comparison; and outputting aging classification, and guiding experiment scheduling operation. According to the method, effective use time efficiency is adopted for rapid judgment, the problem that experimental data is influenced by metabolic degradation of a sample after in-vitro is solved, time efficiency grading can be completed in a short time through multi-marker dynamic comparison and a machine learning model, the method is remarkably superior to a traditional method, and by combining a micro-fluidic detection device and an LSTM model, the detection accuracy is greatly improved. According to the method, the samples can be scheduled to proper experiment operation according to the effective time limit of the samples, waste of the samples is avoided, and after failure samples are detected, a user can be reminded to collect the samples again, so that the experiment process is prevented from being delayed.

The invention relates to the technical field of data center thermal management, and discloses a data center liquid cooling precise temperature control method and system, and the method comprises the following steps: pre-analyzing a calculation task to generate a power consumption prediction sequence; combining the power consumption prediction sequence with the real-time state information of the liquid cooling system, calculating the heat cost of the task at each candidate calculation node through a digital twin model, and selecting a target execution node according to the heat cost; inputting the power consumption prediction sequence and real-time state information into a reinforcement learning controller, and generating a composite coordination action containing a flow regulation instruction and a dynamic power consumption upper limit instruction; and cooperatively controlling the micro-fluidic execution array and the chip power consumption management unit on the target execution node according to the composite coordination action. Through prospective power consumption prediction and two-way cooperative control from the heating end and the heat dissipation end, intelligent distribution and accurate temperature control of thermal loads are achieved, temperature overshoot is effectively restrained, and the overall operation energy consumption of the data center is reduced while the system reliability is guaranteed.

The invention discloses a micro-fluidic liquid cooling heat dissipation module integrated with piezoelectric driving, a chip and application, and belongs to the technical field of chip liquid cooling heat dissipation. The micro-fluidic liquid cooling heat dissipation module comprises a micro-fluidic substrate and a piezoelectric film, the piezoelectric film covers the surface of one side of the microfluidic substrate; a groove channel is formed in the surface of one side, facing the piezoelectric film, of the micro-fluidic substrate; a loop cavity defined by the piezoelectric film and the groove channel forms a micro-channel; a metal electrode layer, a heat source area and a heat exchange area are respectively arranged at different positions, corresponding to the micro-channel, on the surface of one side, deviating from the micro-fluidic substrate, of the piezoelectric film. The micro-fluidic liquid cooling heat dissipation module can actively drive the cooling liquid to flow to a heat source area, and compared with a traditional micro-channel heat dissipation mode, the micro-fluidic liquid cooling heat dissipation module has very high flexibility in fluid driving, does not need an external driving pump, can greatly reduce the size of a packaged chip, and is beneficial to development of chip integration and microminiaturization.

The invention discloses an ultra-high-speed imaging device based on acousto-optic filtering modulation. The ultra-high-speed imaging device comprises a spectrum modulation system, a spectrum imaging system and a data processing system. The spectrum modulation system utilizes an acousto-optic tunable filter (AOTF) to dynamically modulate broadband continuous spectrum light, narrow-band illumination light which rapidly changes along with time is generated, and time information of a dynamic scene is effectively mapped to a spectrum dimension. And the spectral imaging system images the spectral coded dynamic scene to the hyperspectral camera to realize acquisition of spectral space-time information. The data processing system performs spectral crosstalk correction, channel separation and demosaicing reconstruction on the acquired original mosaic image, and recovers a time sequence image according to a spectrum-time mapping relation, thereby realizing single-shot superspeed imaging in a dynamic process. The device has the advantages of being simple and compact in structure, flexible in time window, high in imaging fidelity and the like, and has wide application prospects in the fields of rapid dynamics such as microfluidics, laser processing and ultrafast physics.

The invention relates to the field of pesticide composition screening, and discloses a stabilizer screening process for improving the low-temperature stability of a chlorantraniliprole and lufenuron suspending agent, which comprises the following steps: a) analyzing the low-temperature behavior of chlorantraniliprole and lufenuron based on molecular simulation, constructing a candidate stabilizer molecule library, and preliminarily screening candidate stabilizers; b) applying low-temperature stress to the chlorantraniliprole and lufenuron suspending agent sample containing the candidate stabilizer by using a microfluidic platform, and performing in-situ dynamic characterization to obtain dynamic data related to the low-temperature stability of the sample; and c) processing the dynamic data, and establishing an evaluation index representing the low-temperature stability performance of the candidate stabilizer. According to the invention, a cognitive bridge from a micromechanism to a mesoscopic phenomenon is constructed by integrating molecular simulation pre-screening and microfluidic high-throughput dynamic characterization. The problems of long research and development cycle and resource consumption caused by a large number of trial and error and experience judgment in a traditional screening method are effectively solved.

The invention discloses a cortisol and sodium ion difunctional detection sensor and a preparation method thereof, and belongs to the technical field of sensing detection, the cortisol and sodium ion difunctional detection sensor comprises a substrate layer, a sodium ion detection module and a cortisol detection module, the bionic micro-fluidic unit is provided with a plurality of runner holes, and an embedded capillary pump structure is arranged in each runner hole so as to guide liquid to be detected to the sodium ion detection module and the cortisol detection module; and the shielding layer covers the surface of the bionic micro-fluidic unit. The sodium ion detection module and the cortisol detection module are specifically designed, so that wide-range detection of sodium ion concentration and low-concentration detection of cortisol are realized, a high-altitude low-pressure environment of a pilot can be matched, the sodium ion concentration and the cortisol concentration of the pilot can be accurately detected, and the detection accuracy is improved. Accurate detection of the signs of the pilot in the high-altitude flight process is achieved.

The invention relates to the technical field of microfluidics, in particular to a high-throughput and high-efficiency cell extrusion microfluidic system and a particle introduction method. And a closed-loop control system integrating real-time cell deformation sensing, dynamic parameter decision and physical extrusion and alternating electric field cooperative application is constructed. The chip is provided with an inlet shunting device, a micro-channel and a channel structure of a microelectrode. The method comprises the steps of real-time deformation image acquisition and analysis and dynamic extrusion parameter decision and cooperative application. The invention provides a high-flux and high-efficiency cell extrusion microfluidic system and a particle introduction method, and aims to solve the problems that the drug loading efficiency and the survival rate are difficult to consider due to heterogeneity in the existing cell extrusion technology and a physical extrusion and electroporation coordinated regulation strategy is lacked, so that personalized precise treatment of cells is realized, and the cell quality is improved. The drug loading efficiency, the cell survival rate and the high throughput are obviously improved.

The invention provides a microfluidic device. The microfluidic device comprises a first substrate, a plurality of electrode pairs and a control circuit layer, wherein the first substrate comprises a first surface; the first surface is provided with a plurality of opening areas arranged at intervals, each opening area is provided with two containing grooves arranged at intervals in the first direction, the common side wall of the two containing grooves is provided with a micro-flow channel, and the two containing grooves are communicated through the micro-flow channel. The plurality of electrode pairs are arranged on the first substrate; each electrode pair is arranged corresponding to one opening area; the electrode pair comprises two driving electrodes, and the two driving electrodes are arranged on the two opposite sides of the microfluidic channel at intervals in the first direction. The control circuit layer is arranged on the first substrate and is electrically connected with the plurality of electrode pairs; wherein the control circuit layer is used for applying voltage to each electrode pair. According to the microfluidic device, a plurality of DNA molecules or biological samples are processed in parallel, and the detection efficiency is remarkably improved.

The invention belongs to the field of photoelectric co-packaging, and particularly relates to a photoelectric co-packaging temperature control framework based on an embedded glass-based micro-channel. Independent lower-layer heat dissipation is carried out on a heat-sensitive component optical interconnection chip through an embedded glass-based micro-channel technology, an electric interconnection heat dissipation channel continues to use existing upper-layer heat dissipation, mutual isolation of two heat dissipation channels in the whole common packaging body is achieved, heat dissipation partition is achieved from a new dimension, and thermal crosstalk is avoided; the whole common packaging body dissipates heat at the upper layer and the lower layer, so that the effective heat dissipation area is greatly increased, and the heat dissipation capability of the packaging body is greatly improved; and the guidance quality of the heat dissipation channel is better through material selection and additional arrangement of the heat insulation layer. And a metal film temperature sensor can be additionally arranged, so that the temperature of a component can be monitored in real time, heat management regulation and control can be performed in a targeted manner, the temperature rise of a heating device and the heat contact efficiency between the micro-channels are balanced, and more efficient heat dissipation is realized.

The invention discloses a method and a system for improving carbon dioxide mineralization and storage efficiency. The method comprises the following steps: building a microfluidic experiment platform integrated with an online CO2 mineralization and storage monitoring system; on a microfluidic experimental platform, simulating a formation temperature and pressure condition to carry out a CO2 mineralization corrosion experiment to obtain pore scale reaction kinetic parameters and a pore structure evolution rule; macromineralization efficiency data are obtained through an indoor physical simulation mineralization reaction experiment of the core scale; a CCM-GEM reaction flow numerical model is constructed; determining a Pareto optimal solution set by using a CMG-GEM reaction flow numerical model; and based on the pore scale reaction kinetic parameters, the pore structure evolution law, the macromineralization efficiency data and the Pareto optimal solution set, determining a CO2 mineralization storage efficiency improvement path of the target rock sample. According to the method, global sensitivity analysis and automatic optimization of multiple parameters are rapidly completed, the research and development period is shortened, the economic cost and the time cost are reduced, and the research efficiency is improved.

The invention discloses a breath anomaly detection method and system based on gas monitoring, and relates to the technical field of breath anomaly detection.The breath anomaly detection method comprises the steps that mouth and nose gas signals in the breath process of a user are collected and preprocessed, and gas component separation is conducted through a microfluidic gas separation module; and performing differential analysis on separated gas components, calculating fluctuation of nasal cavity and oral cavity gas components, performing breathing mode recognition through 1D SNN, and establishing a personalized breathing mode library in combination with long-term health data of a user. Compared with the prior art, the method has the advantages that more accurate breathing mode recognition and anomaly detection are realized by combining differential analysis of multi-dimensional gas components and a deep learning model with a personalized breathing mode library, so that a more intelligent health management scheme is provided, and particularly, the method has remarkable advantages in coping with complex personalized differences and long-term health trends.

The invention discloses a microfluidic nitration reaction platform based on in-situ Raman and a reaction optimization method. The platform integrates a microreactor, an in-situ Raman detection module, a spectrogram processing and quantifying module, an experimental optimization module and a central control module, and is suitable for monitoring and optimizing complex reactions under a non-mutual solution phase plunger flow system. According to the method, an indirect hard modeling (IHM) method is used for processing an overlapped Raman spectrogram, a standard curve is combined for establishing a multi-component concentration prediction model, and high-precision quantification of reactants and products is realized. According to the platform, a Gaussian process model is combined with dynamics prior, a multi-target Bayesian optimization process based on expected hypervolume improvement (EHVI) is driven, and Pareto optimal conditions in a temperature and residence time space are effectively explored. The system has the functions of automatic focusing sampling, spectrogram real-time decoupling, concentration inversion and experimental condition decision making, and is suitable for intelligent reaction development and optimization in the fields of fine chemical engineering, high-throughput reaction screening and the like.

The invention relates to the technical field of biomedical engineering and microfluidics, in particular to a fluid dynamics topological optimization design method of a circulating tumor cell sorting structure, which comprises the following steps of: establishing a three-dimensional flow field model containing a key separation area in a preset design domain, dispersing by adopting a tetrahedral mesh and setting boundary conditions; the method comprises the following steps: establishing a multi-objective optimization function based on a maximized transverse focusing distance, a minimum shear force peak value and a total pressure drop, dispersing a design domain into units and endowing the units with density variables, and realizing global automatic topological optimization by adopting penalty material interpolation and minimum flow velocity threshold constraint. An optimization result is subjected to structural reconstruction processing to generate a CAD model adaptive to micro-nano machining, closed-loop feedback is formed through secondary verification and experimental verification of computational fluid mechanics, it is ensured that the design meets the requirements for sorting efficiency and cell activity, limitation of a traditional flow channel form is broken through, and the design freedom degree, manufacturability and application value are improved.

The invention discloses a silicon carbide power module, which comprises a top diamond micro-channel heat dissipation layer, a bottom diamond micro-channel heat dissipation layer, an upper diamond layer, a middle diamond layer and a lower diamond layer, and is characterized in that an upper current conducting layer is arranged between the upper diamond layer and the middle diamond layer; a lower current conducting layer is arranged between the middle diamond layer and the lower diamond layer, a plurality of power chips are embedded in the middle diamond layer, and the power chips are electrically connected with the upper current conducting layer and the lower current conducting layer. According to the silicon carbide power module, the diamond heat dissipation layers integrated with the micro-channels are arranged at the top and the bottom respectively, the heat exchange efficiency and the heat dissipation effect of the power module can be effectively improved, an external heat dissipation device is not needed, the structure is simpler, special heat dissipation can be conducted on the area where the power chip is located, and the service life is prolonged. And the purposiveness and the effectiveness of heat dissipation are higher.

The invention relates to the technical field of intelligent control of double-drum washing machines, and discloses an intelligent control system of an energy-saving double-drum washing machine, which comprises a hardware execution unit used for providing multi-mode sensing, accurate liquid management, mechanical execution and energy recovery functions; the control processing unit is used for processing the received data, operating an algorithm and coordinating the operation of each component of the system; the cooperative control software module runs on the main control board and comprises an acoustic fingerprint identification and fault diagnosis module and an intelligent fluid precise counterweight and cooling module; an AI-driven collaborative decision and adaptive optimization module; the invention discloses a micro heat pump energy recovery and precise temperature control linkage module. By means of the high-sensitivity acoustic array sensor and the acoustic fingerprint recognition and fault diagnosis module, clothes imbalance is recognized, point-to-point liquid balance weight is achieved by means of the micro-fluidic spraying valve array, the optimal dehydration cooperative scheduling strategy is generated, water and electricity resources are greatly saved, and the dehydration efficiency is improved.

The invention relates to the technical field of composite hydrogel preparation, in particular to a nano composite hydrogel preparation process based on a microfluidic technology. The gel comprises the following components in parts by mass: 50-70 parts of a gel matrix, 5-9 parts of a nano enhancement system, 8-14 parts of a functional carrier, 3-6 parts of a dynamic response system, 4-7 parts of a hyaluronic acid-sulfhydrylated heparin compound and 0.5-1.5 parts of L-ascorbyl palmitate. Precise mixing and directional arrangement of all component materials on a micro-level are realized through a microfluidic technology, so that a nano reinforcing system and a functional carrier are uniformly distributed in a gel matrix, the synergistic effect of all the components is fully exerted, the mechanical property and the biological function of the gel are effectively improved, and the preparation method is suitable for industrial production. And quaternized chitosan nanofibers, a dynamic response system for responding to external environment change and a hyaluronic acid-sulfhydrylated heparin compound for promoting angiogenesis are introduced, so that the gel has the biological functions of resisting bacteria and promoting angiogenesis through a synergistic effect.

The invention provides a detection method of an end face integrated reflection type SPR optical fiber biosensor manufactured based on a 2PP technology. An experiment system mainly comprises a single-mode optical fiber, an optical fiber coupler, an SPR probe, a tungsten lamp light source, a sample pool, a spectrum analyzer and a computer. The method is characterized in that a volcanic three-dimensional microstructure is directly constructed on the end face of a single-mode fiber through a 2PP technology, a metal film with the thickness of about 50nm is deposited on the inner wall of the volcanic three-dimensional microstructure, and a stable and efficient SPR excitation structure is formed; the structural parameters are subjected to FDTD simulation optimization design, and have the characteristics of high quality factor and high sensitivity. The sensor can be used for AFP detection, the sensitivity reaches 0.684 nm / (ng / mL) within the linear range of 0-10 ng / mL, the normal and risk early warning concentration interval of human serum AFP is covered, and the sensor has good biological suitability and early hepatocellular carcinoma screening capacity; in addition, the probe structure is small in size, detection can be completed only by a few microliters of liquid to be detected, and the probe is suitable for a microfluidic platform with an extremely low sample size or a clinical sampling scene. Compared with the prior art, the device has the advantages of high structural integration, high sensitivity, low sample consumption, convenience in manufacturing, stability, reliability and the like.

The invention relates to a microfluidic reactor for electrocatalytic determination of chemical oxygen demand, and belongs to the field of environmental monitoring. In the reactor, to-be-detected samples are injected from a sample inlet positioned at the front end of a liquid inlet and flow into the reactor formed by stacking three layers of components from the liquid inlet below along with electrolyte, and the to-be-detected samples positioned at different horizontal heights turn along with liquid flow and finally reach the surface of a planar anode at the bottom under the action of a pinch plate distributed with a micro cone group; and the water flows upwards through a hole in one end of the top side of the buckle plate, passes through the auxiliary electrode with the hole and finally flows out of the electrolytic tank through a water outlet in the top. Organic matters in the to-be-detected sample are subjected to electrochemical oxidation reaction under the action of the catalyst on the surface of the planar anode at the bottom of the reactor, and a generated electric signal is used for quantitatively determining the chemical oxygen demand of the to-be-detected water sample. In the invention, the pinch plate distributed with the micro cone group can strengthen organic matters to break through stable horizontal laminar mass transfer, so that the organic matters in the sample to be detected fully reach the surface of the plane anode to generate electrochemical oxidation reaction, thereby improving electric signals and lower limit of detection. According to the characteristics, a more stable and accurate electric signal can be obtained when the reactor is used for measuring the chemical oxygen demand, and the linear range of the measurement is widened, so that the efficient and accurate measurement of the chemical oxygen demand in a sample to be measured is realized.

The invention relates to the technical field of atmospheric biological aerosol sampling and detection, in particular to a particle size resolution type aerosol in-situ sampling and analysis integrated system and method. The system comprises an electrostatic field grading aerosol sampler, a micro-fluidic detection chip based on an immunochromatography principle and an image acquisition and data analysis unit, according to the electrostatic field grading aerosol sampler, biological aerosol particles are electrified through a charge tube, and grading capture according to particle sizes is realized in a stepped electrostatic field; the micro-fluidic detection chip transfers an enriched sample to a multi-layer structure micro-fluidic chip, and a specific reaction is completed based on an immunochromatography principle; and the image acquisition and data analysis unit captures a color developing image through a high-definition camera, and adopts an RGB analysis algorithm to realize quantitative detection. The method realizes full-process automation from sampling to analysis, solves the problems of low efficiency and tedious operation of a traditional method, and has the advantages of high detection speed, high sensitivity, no need of harmful reagents, environmental friendliness and the like.

The invention relates to the technical field of micromolecule mineral water monitoring, and discloses a micromolecule mineral water quality monitoring system based on an intelligent sensor. The system comprises a multi-channel spectrum sensor array, an ion selective field effect transistor group, a microfluidic preprocessing module, a dynamic compensation database, a self-adaptive analysis engine, a cloud verification interface and a feedback execution mechanism. The micro-fluidic pretreatment module is used for eliminating bubbles of an original water sample, screening particles and outputting stable liquid flow; the spectrum sensor array captures full-band absorption spectrum data to generate a characteristic matrix, and the transistor group detects concentration fluctuation of calcium ions, magnesium ions and silicate; the adaptive analysis engine performs multi-modal fusion and abnormal marking on the time sequence alignment data stream; the cloud interface performs cross validation on the local result and the regional water quality center reference library to generate a credibility report; and the feedback execution mechanism adjusts the microfluidic flow velocity and the sensor integration time according to a report to realize efficient water quality monitoring.

The invention relates to the technical field of online detection and analysis, and discloses a bone joint synovial fluid detection and analysis system based on microfluidics, which specifically comprises the following steps: acquiring synovial fluid rheological data and synovial fluid molecular activity data in a bone joint movement process and cartilage surface morphological data in a joint buckling process through a biosensor; constructing a synovial fluid dynamic anomaly analysis model, and performing synovial fluid function anomaly analysis through the synovial fluid rheological data and the molecular activity data; a cartilage stress anomaly analysis model is constructed, and cartilage stress anomaly analysis is carried out through cartilage surface morphological change data; obtaining a synovial fluid function abnormality analysis result and a cartilage stress abnormality analysis result to carry out bone joint function adaptability analysis; and acquiring a bone joint function adaptability analysis result and carrying out synovial fluid function abnormality analysis early warning. The problem that in the prior art, due to the fact that the bionic degree of a detection strategy is insufficient and bone joint dynamic parameters are deficient, the association degree of a static synovial fluid detection result and the actual joint rehabilitation condition of a patient is poor is solved.

The present application relates to the technical field of melamine phase change microcapsule preparation, and particularly relates to a melamine phase change microcapsule preparation device and method based on microfluidic technology; the technical scheme: a melamine phase change microcapsule preparation device and method based on microfluidic technology, comprising an aqueous solution tank, an aqueous phase liquid inlet pump, a first valve, a pressure regulator, an oil phase liquid inlet assembly, a liquid mixing assembly, a reaction assembly and a collection assembly; compared with the traditional melamine phase change microcapsule preparation method, the melamine phase change microcapsule prepared by the intermittent method has the problems of poor quality stability, low performance reproducibility, low production efficiency and low cost, etc.; the melamine phase change microcapsule preparation method adopts a microfluidic device integrating temperature control, liquid mixing and chemical reaction, etc., and by connecting the liquid inlet pump at different places, various components can be introduced and polymerization reaction can be carried out in the process of preparing the melamine phase change microcapsule.

The invention discloses a system and a method for rapidly detecting trace active ingredients of a medicine, belongs to the technical field of medicine detection, and relates to cross application of surface enhanced Raman spectroscopy and artificial intelligence. The system comprises a sample preparation module, a micro-fluidic enrichment module, a Raman spectrum acquisition module, a self-adaptive spectrum analysis module, a multi-component identification module and an intelligent report generation module, and efficient enrichment and nanogram-level detection of drug molecules are realized through an enhanced surface Raman scattering micro-fluidic chip. Intelligent identification and accurate quantification of complex spectral characteristics are realized by adopting a deep learning algorithm and a self-adaptive weight calculation method, synchronous detection of 5-10 active pharmaceutical ingredients is supported, the detection sensitivity reaches nanogram / milliliter level, the quantification error is less than 5%, the detection time of the whole process is 5-10 minutes, and the detection accuracy is high. The sensitivity, the accuracy and the efficiency of medicine quality detection are obviously improved.

The invention discloses an intelligent design and precise preparation method of structure-controllable non-spherical particles based on a microfluidic technology, and relates to the field of microfluidic technology and artificial intelligence cross application, and the method comprises the following steps: S1, realizing continuous preparation of various non-spherical particles based on a microchannel confinement effect, and obtaining a data set by combining high-throughput numerical simulation; s2, constructing a controllable prediction model matched with various non-spherical liquid drops, and training and verifying the controllable prediction model based on the data set; s3, selecting a corresponding controllable prediction model based on the particle shape, inputting device parameters, physical property parameters and target deformation by a user, and performing reverse search and iterative optimization to obtain operation working conditions; and S4, completing preparation of non-spherical particles based on the predicted spherical droplet template size and working conditions. According to the reverse intelligent design strategy based on artificial intelligence, target-oriented non-spherical particle micro-fluidic precise preparation is achieved, and the micro-fluidic preparation efficiency of the non-spherical particles is greatly improved.

The invention discloses a high-flux organoid culture liquid core device which comprises an upper cover module, a transwell module and a porous culture plate, more than one culture hole is formed in the porous culture plate, a liquid inlet hole and a liquid outlet hole are formed in the bottom of each culture hole, at least one culture liquid circulation channel is formed in the porous culture plate, and the culture liquid circulation channels are communicated with the culture holes. The culture solution circulation channel comprises an injection port, an input channel, a discharge channel and a discharge port, the injection port is communicated with the input channel, and the discharge channel is communicated with the discharge port; the porous culture plate comprises a plurality of culture holes and a plurality of culture solution circulation channels, each culture solution circulation channel is communicated with an injection port and a discharge port of at least one culture hole, the transwell module comprises more than one transwell chamber, the transwell chambers are matched with the culture holes and placed on the culture holes, and the upper cover module is used for sealing and covering the porous culture plate. According to the liquid core device, a traditional culture plate and a micro-fluidic technology are combined, high-throughput culture of the organoid is achieved, and the homogeneity of the organoid culture tissue is improved due to the fact that a parallel multi-group organoid culture mode is adopted.