363 results about "Protein detection" patented technology

The present invention belongs to the field of biological detection technology, specifically relating to a monoclonal antibody combination for detecting HPV18 E7 protein and its application. This combination consists of monoclonal antibodies 5F8 and 6G6, wherein the heavy and light chain variable regions of 5F8 and 6G6, respectively, contain specific complementarity-determining region sequences (as shown in SEQ ID NOs. 1 to 12), conferring high specificity for HPV18 E7 protein. Verification using a double-antibody sandwich ELISA and a biotin-avidin amplification system demonstrated that this antibody combination specifically recognizes HPV18 E7 protein, exhibits no cross-reactivity with other high-risk HPV oncoproteins, and exhibits a detection sensitivity of 1 ng / mL. This combination can be used to construct detection systems such as ELISA kits and immunochromatographic test strips, suitable for rapid screening of HPV18 E7 protein in cervical exfoliated cell samples.

Provided are an apolipoprotein E detection reagent and the use thereof. The reagent comprises at least one of an ApoE2 protein detection reagent, an ApoE3 protein detection reagent and an ApoE4 protein detection reagent. One of a capture antibody and a detection antibody used for detecting ApoE2 protein or ApoE4 protein is a specific monoclonal antibody, and the capture antibody and the detection antibody used for detecting ApoE3 are both specific monoclonal antibodies. Genotyping of ApoE and quantitative detection of different genotypes of proteins are performed on the basis of an immunological detection method. The detection method has the advantages of being simple, easy to operate, short in time and cheap. A genotype detection result is highly consistent with a fluorescence PCR result. Genotyping of ApoE (6 types) can be performed, which is used to replace the nucleic acid detection and guide medication, and clarify the correlation of the ApoE4 genotype homozygosity / heterozygosity and the protein concentration of ApoE4 with AD.

The invention belongs to the field of biomedicine, and particularly relates to application of CRYL1 protein in preparation of a product for evaluation, diagnosis or auxiliary diagnosis of postoperative recurrence risk of calcium oxalate kidney stone combined with renal nipple calcium spot, and the sequence of the CRYL1 protein is shown as SEQ ID NO.1. The invention discovers that the expression of the CRYL1 protein in renal tubules and collecting tube cells of renal papillary calcium spot tissues is reduced for the first time, and tests prove that the urine CRYL1 protein is a predictive factor and a predictive marker for postoperative recurrence of a patient with CaOx kidney stone combined with renal papillary calcium spot. The CRYL1 protein detection kit is further researched and developed to be used for detecting or evaluating the postoperative recurrence risk of patients suffering from CaOx kidney stone combined with renal nipple calcium spots, personalized follow-up visit and prevention schemes of the patients are promoted, and the CRYL1 protein detection kit has huge clinical application prospects.

The invention belongs to the technical field of biological detection, and relates to an antibody composition aiming at pTau217 protein detection and application thereof, and the antibody composition aiming at pTau217 protein detection comprises a target pTau217 protein antibody and a target Tau protein C-terminal antibody. Meanwhile, based on the antibody composition aiming at pTau217 protein detection, the invention further provides a kit for early diagnosis of the Alzheimer's disease. The invention provides an antibody composition aiming at pTau217 protein detection, which is stronger in detection specificity and higher in sensitivity, and an application of the antibody composition.

The invention is applicable to the technical field of C-reactive protein detection, and provides an anti-C-reactive protein monoclonal antibody idiotype antibody, a preparation method and application, and the anti-C-reactive protein monoclonal antibody idiotype antibody is secreted by a hybridoma cell strain; the hybridoma cell strain is a 7C4 hybridoma cell strain; the preparation method of the anti-C-reactive protein monoclonal antibody idiotype antibody comprises the following steps: S1, obtaining hybridoma cells: preparing an anti-C-reactive protein monoclonal antibody Fab fragment, then carrying out animal immunization, then carrying out cell fusion, and finally screening and cloning the hybridoma cells; and S2, preparing and screening an anti-C reactive protein monoclonal antibody idiotype antibody. The anti-C-reactive-protein monoclonal antibody idiotype antibody is used for replacing C-reactive protein to serve as a labeled antibody, the labeling process is simple, the labeling efficiency is high, and meanwhile the situation that a final product is affected due to instability of the C-reactive protein is avoided. Experimental results show that the detection method is good in specificity and wide in linear range.

The invention discloses a magnetic particle chemiluminiscence detection kit for human non-phosphorylated Tau217 protein and a preparation method of the magnetic particle chemiluminiscence detection kit. The kit comprises a micro magnetic particle coupled with a human non-phosphorylated Tau217 protein capture antibody, an acridinium ester labeled human non-phosphorylated Tau217 protein detection antibody, a human non-phosphorylated Tau217 protein calibration product and a quality control product. The direct chemiluminescence method established by the invention has the advantages of high sensitivity, strong specificity, accuracy, rapidness and short detection time, the detection result has higher accuracy and repeatability, and the kit can be used for various luminescence detection instruments.

The invention discloses a Claudin18.2-targeting nano antibody and application thereof, and belongs to the technical field of molecular biology. Aiming at the unique advantages of a nano antibody in the prior art, the invention provides a nano antibody targeting Claudin18.2, the amino acid sequence of the targeting nano antibody is shown as SEQ ID NO.1, and the nano antibody has relatively strong affinity with Claudin18.2 positive cells 293T-Claudin18.2, and can be combined with Claudin18.2 protein expressed on the surfaces of the cells. The targeted Claudin18.2 nano antibody provided by the invention can be prepared through in-vitro engineering bacterium mass expression, can be applied to preparation of protein detection antibodies or therapeutic antibodies, can be used for in-vivo and in-vitro detection of tumor tissues expressed by Claudin18.2, and has important commercial value in clinical disease diagnosis and treatment.

The invention discloses a hydrophobic magnetic bead, a mixed magnetic bead and a preparation method and application thereof, and a protein detection method. The preparation method of the hydrophobic magnetic bead comprises the following steps: S1, carrying out hydroxyl modification on the surface of a magnetic bead to obtain a hydroxyl magnetic bead; s2, performing double-bond modification on the surfaces of the hydroxyl magnetic beads to obtain double-bond magnetic beads; s3, performing carboxyl modification on the surfaces of the double-bond magnetic beads to obtain hydrophobic magnetic beads; wherein the carboxyl modification comprises the following step: carrying out free radical addition reaction on a mixed solution of double-bond magnetic beads, sulfydryl carboxylic acid, an initiator and a solvent. The obtained hydrophobic magnetic beads have high protein binding rate and high stability; and after further mixed use with hydrophilic magnetic beads, unbiased extraction of protein in a complex biological sample is realized, and higher adaptability and lower cost are achieved.

The invention belongs to the technical field of immunochemistry, and particularly relates to an anti-PR recombinant rabbit monoclonal antibody and application thereof.The anti-PR recombinant rabbit monoclonal antibody comprises a heavy chain variable region and a light chain variable region, and the amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 4; the amino acid sequence of the light chain variable region is as shown in SEQ ID NO: 5. The invention also relates to a nucleotide sequence for coding the anti-PR recombinant rabbit monoclonal antibody, a recombinant plasmid or an expression vector, a preparation method, application of the anti-PR recombinant rabbit monoclonal antibody in a PR protein detection method or device, and the like. The PR recombinant rabbit monoclonal antibody disclosed by the invention has the characteristics of good specificity, strong positive signal and the like, so that scoring is easier in IHC staining, and cancer detection and distinguishing are more accurate.

The present disclosure relates to proteomics, including the detection of immune cell target proteins as examples that the disclosed methods can be used for protein detection. Methods, compositions, and systems are described for identifying the presence of proteins, including detecting immune cell proteins from a sample using a combination of multiplex immuno-PCR with antibodies conjugated to oligonucleotides comprising an antibody-specific barcode flanked by PCR priming sites; detection of barcoded amplicons obtained from immuno-PCR with encoded padlock probes comprising codes specific for the barcodes / target protein; rolling circle amplification (RCA) of the circularized padlock probes; and determination of the sequence of the codes amplified by RCA by next generation sequencing or using fluorescently labeled hybridization probes.

The invention belongs to the technical field of bioengineering, and particularly relates to a nucleic acid aptamer specifically bound with MMLV, and the nucleotide sequence of the nucleic acid aptamer is as shown in SEQ ID NO.1 or SEQ ID NO.2; the nucleic acid aptamer capable of being specifically bound with MMLV is obtained through screening on the basis of the SELEX technology, has high affinity for MMLV protein, can be applied to MMLV protein enrichment reagents, MMLV protein separation reagents, MMLV protein detection reagents, test paper and biosensors, and facilitates identification and activity research of the MMLV protein.

The invention discloses a viral ARDS prognosis evaluation system based on protein joint detection, and the system comprises a sample processing module which is used for carrying out serum standardization pretreatment; the protein detection module is used for performing iBAQ quantification of IL6ST / FOXO3 / TLR7 based on DIA mass spectrometry, defining a targeted therapy threshold value and realizing targeted quantification of the core protein; and the intelligent analysis module is used for realizing cross-age risk layering based on a multivariable logic regression model. B cell function states are reflected by combining serum IL6ST / FOXO3 / TLR7 protein expression levels, a multivariable logistic regression model is constructed, and unified risk stratification of patients of all ages from children to the elderly is achieved; by locking an IL6ST / FOXO3 / TLR7 pathway, a targeted therapy threshold is defined to directly guide targeted therapy, and immune intervention is facilitated.

The invention provides a protein and metal ion binding site identification method, device and equipment, and belongs to the field of protein detection.The method comprises the steps that feature extraction is conducted on known metal ion binding protein, and multiple sample evolution information features are obtained; the method comprises the following steps: for an unknown protein sequence, determining candidate distant homologous metal ion binding proteins through multi-sequence comparison and cosine similarity screening, and further constructing a training set; a composite framework in which a bidirectional long-short-term memory network and a full-connection neural network are connected in series is adopted, input features comprise evolutionary information features and physicochemical attribute features, and probability values of binding sites of different metal ions are output; training the composite framework through the training set to obtain a site prediction model; and predicting a binding site of an unknown protein sequence by using the prediction model. The stable and efficient prediction on the metal ion binding site is realized.

The invention belongs to the technical field of biological detection, and relates to an antibody composition aiming at human pS129-alpha-Syn protein detection and application thereof, and the antibody composition comprises a targeted pS129-alpha-Syn protein phosphorylation antibody and a targeted human alpha-synuclein N-terminal antibody. Meanwhile, based on the antibody composition for detecting the human pS129-alpha-Syn protein, the invention further provides a kit for early diagnosis of the Parkinson's disease. The invention provides an antibody composition aiming at human pS129-alpha-Syn protein detection and application of the antibody composition. The antibody composition is higher in detection specificity and sensitivity.

The invention discloses a patterned hydrophilic and hydrophobic fluorescence enhancement chip, a preparation method thereof and a protein detection method, and relates to the technical field of protein detection. Hydrophilic and hydrophobic patterns are designed on the basis of the plasma fluorescence enhancement chip with gold as a substrate, protein micro-droplets and to-be-detected sample droplets are confined through the hydrophilic and hydrophobic patterns, the method does not depend on large sample application equipment and expensive chip fences, and the method has the advantages of being easy to operate and high in sensitivity. Experimental results show that when the patterned hydrophilic and hydrophobic fluorescence enhancement chip provided by the invention is used for detecting protein, the use amount of a sample for manual sample application is small, and the detection sensitivity is high.

The present disclosure relates to an azide-modified fluorescent π-conjugated polymer and a DNA-modified π-conjugated fluorescent nanoparticle-based protein detection imaging method capable of signal rapid amplification and high throughput. The present disclosure prepares oligonucleotide-modified fluorescent π-conjugated polymers (OCPNs) based on the azide-modified fluorescent π-conjugated polymer. The OCPNs have ultrahigh fluorescence luminance and extremely high detection sensitivity, and can complete detection and imaging of targets in different abundances without the need of an additional fluorescence signal amplification step. In addition, a rapid and specific targeting effect can be achieved with an extremely small dosage and an entire staining and imaging process can be shortened, so that the OCPNs achieve an extremely high imaging efficiency during multi-color imaging and thus exhibit great application prospects.

The invention belongs to the technical field of protein calibration, and particularly relates to an enhanced protein-free confining liquid for protein detection, which comprises the following components: trihydroxymethyl aminomethane, sodium chloride, Tween-20, polyvinyl alcohol, trehalose, EDTA (Ethylene Diamine Tetraacetic Acid) and a polyoxyethylene-polyoxypropylene block copolymer, and sodium chloride and trehalose are matched to provide ions and small molecular compounds to form a stable closed environment. Wherein the trehalose assists in filling and covering small pores of PVDF, the sodium chloride provides ion balance, the EDTA-Na is used for precipitating metal ions, and the Tween-20 assists in repairing the PVDF film. According to the invention, the polyvinyl alcohol and the polyoxyethylene-polyoxypropylene block copolymer are mixed for use, so that non-specific sites on the membrane are blocked while impurity proteins are prevented from being introduced, the sealing effect is achieved, and the problem of false positive or non-specific binding easily caused by the traditional sealing liquid is effectively solved.

The invention belongs to the technical field of biological detection, and particularly relates to a specific monoclonal antibody for AKK bacterium active protein P9, a detection kit and application of the specific monoclonal antibody. The antibody pair is 7G4 and 8B2, the amino acid sequence of a heavy chain variable region of the 7G4 is SEQ ID NO.2, and the amino acid sequence of a light chain variable region of the 7G4 is SEQ ID NO.4; the amino acid sequence of the 8B2 heavy chain variable region is SEQ ID NO.6, and the amino acid sequence of the 8B2 light chain variable region is SEQ ID NO.8. The antibody pair has strong specificity and high affinity, and can accurately recognize different antigen epitopes of the P9 protein. A colloidal gold detection and double-antibody sandwich ELISA detection system constructed based on the kit adapts to a complex matrix and various process treatment samples, is stable and reliable in detection, has qualitative and quantitative capabilities, can be used for scenes of P9 protein detection, fermentation monitoring, quality control and the like, and solves the problems of insufficient specificity, poor adaptability and the like in the prior art.

The invention discloses a probe Cvs-B-NHS based on a coumarin derivative as well as a preparation method and application of the probe Cvs-B-NHS. According to the fluorescent probe Cvs-B-NHS of the coumarin derivative, a coumarin mother nucleus and succinimide ester are organically combined, the excellent fluorescence characteristic of coumarin is reserved, efficient protein labeling capacity is achieved, high-sensitivity fluorescence signal output and specific labeling of protein are achieved, and the fluorescent probe Cvs-B-NHS has the advantages of being high in sensitivity and high in sensitivity. And the accuracy and reliability of protein detection and imaging are improved. The invention discloses a TDP-43 protein phase separation labeling method based on a Cvs-B-NHS probe, which provides a new means for researching the action mechanism of a TDP-43 (G335D) mutant in neurodegenerative diseases, and is beneficial to promoting the research progress in the field of neurodegenerative diseases. The application of the Cvs-B-NHS probe in evaluating the polarity change of the microenvironment in the protein liquid-liquid phase separation process in the cytoplasm environment caused by mitochondrial signal disturbance provides a powerful tool for the research of the protein phase separation microenvironment.

The invention relates to the technical field of protein detection, in particular to a quantitative detection method for the content of soybean protein components. According to the quantitative detection method for the component content of the soybean protein, the capillary electrophoresis method is adopted, the detection method is optimized, efficient separation of all components of the soybean protein can be achieved, quantitative calculation can be achieved, the analysis time is short, the scheme is reliable, and reproducibility is good.

The invention discloses a protein quantitative detection method which comprises the following steps: respectively constructing a capture probe for capturing antibody coupling magnetic beads and oligonucleotides and a detection probe for detecting antibody coupling oligonucleotides through a biorthogonal chemical coupling method; then, target protein is captured by using a biorthogonal chemical coupling and magnetic bead sandwich method comprising the steps of capture probe combination, closing, washing, detection probe combination and the like, and accurate quantification of low-abundance protein in a complex sample is realized by combining a subsequent digital PCR (Polymerase Chain Reaction) technology.

The invention discloses an FLG gene and protein detection method based on oral cell non-invasive sampling, which comprises the following steps: Step 1: sample collection: collecting oral cells through mouth rinsing of a subject; step2: RNA extraction and gene expression analysis: extracting oral cell total RNA in the oral cells in the step 1 by using a Trizol reagent, and analyzing FLG gene expression through qRT-PCR (Quantitative Reverse Transcription-Polymerase Chain Reaction); 3, protein extraction and immunoblotting analysis are conducted, specifically, protein is extracted from the oral cells in the step 1 through an SDS lysis buffer solution, and FLG protein expression is detected through the Western blot technology. According to the present invention, the subject rinses the mouth and collects the oral cavity cells, such that the convenient, non-invasive and efficient sample collection is achieved.

The application discloses a preparation method of a VP@UiO-66-NH2 / Ag NPs composite SERS chip and application thereof in A beta protein detection, and is characterized by comprising the following steps: synthesizing a VP-graphite paper base by a chemical vapor transport method on a surface of a graphite paper to obtain a VP-graphite paper base; synthesizing a UiO-66-NH2 layer on the surface of the VP-graphite paper base by a solvothermal method to obtain a VP@UiO-66-NH2 composite base; and finally, placing the composite base in an ion sputtering instrument, uniformly sputtering silver nanoparticles on the surface of the composite base, taking out the composite base, and obtaining the VP@UiO-66-NH2 / Ag NPs composite SERS chip, wherein the VP@UiO-66-NH2 / Ag NPs composite SERS chip has the advantages of high sensitivity, good stability and uniformity, controllable preparation process, low cost, application in Alzheimer's disease biomarker detection, high specificity in identification and quantitative analysis of A beta 40 and A beta 42 proteins, fast response and good stability.

The invention relates generally to peptide biomarkers with specific ionization characteristics to directly quantify one or more target HPPD proteins in biological samples, including crop plant samples, by liquid chromatography coupled tandem mass spectrometry multiple reaction monitoring (MRM). The peptide biomarkers in combination with MRM-based methods may be used to quantify a single target protein or multiple target proteins within a crop plant, such as maize, utilizing selected peptide biomarkers either alone or in combination. The present disclosure allows for broad based, reliable quantitation in different biological matrices, including plant matrices. Also provided are different peptide biomarker combinations that can be used to perform the methods of the invention.

The present invention provides a set of sequences of oligonucleotide aptamers that specifically recognize the radiation-sensitive protein CDKN1A and a detection method, belonging to the technical field of protein detection. In the present invention, two specific aptamers were first screened for the radiation-sensitive protein CDKN1A through the SELEX technique, and the nucleotide sequences of the corresponding oligonucleotide aptamers are shown as SEQ ID No.1 and SEQ ID No.2. Both of the two oligonucleotide aptamers of the present invention can specifically recognize the CDKN1A protein without binding to other irrelevant proteins. The present invention also established a new detection method for the CDKN1A protein by using the principle of the microplate detection method, which can be used for radiation dose assessment and cell senescence identification. The detection method of the present invention has the advantages of high sensitivity and short detection time, and there is also a need to develop more applicable detection methods.

According to the method for detecting the conductance of the protein molecules and the application of the method, asymmetric modification is carried out on the tunneling electrode pair and the protein molecules, so that the protein molecules can enter a tunneling area in a fixed posture and form asymmetric single-molecule junctions with the tunneling electrode pair, the activity of the protein molecules can be kept to the maximum extent, and the conductivity of the protein molecules is detected. Therefore, the device capable of stably detecting the conductance of the protein molecules for a long time can be constructed and obtained, and the device can be used for researching the dynamic conformation of the protein, screening small-molecule drugs targeting the protein molecules and analyzing the interaction relationship between the small-molecule drugs and the protein molecules. The tunneling electrode device and the real-time electrical measurement technology are used for achieving protein detection on the single molecule level, the conformation change process of protein such as biological enzyme and the action characteristics of the protein and small molecules are comprehensively described on the high time resolution scale, and the design and optimization efficiency and success rate of existing drugs are expected to be improved.

The invention belongs to the technical field of biological detection and analysis, and particularly relates to a high-fidelity biotinylation probe as well as a preparation method and application thereof in polysulfide detection. Specifically, the invention develops a novel biotinylation probe HPB, and the probe is formed by coupling HPE-IAM and biotin. Experimental results show that HPB is superior to IAB and other traditional reagents in the aspects of maintaining and efficiently labeling polysulfide, and a high-specificity solution is provided for Pr-SnH analysis at the proteomics level. By optimizing a marking scheme and introducing a precipitation-redissolution step, the detection accuracy of the polysulfide modified protein is further improved, so that a reliable tool is provided for protein polysulfide modification detection research under a complex cell background, and the method has a good practical application value.

The present invention relates to the field of biomedical analysis, and discloses a CHO-S cell residual protein detection kit and a preparation method thereof, including an enzyme-labeled plate coated with an anti-HCP protein antibody, biotin-labeled IgG, streptavidin labeled with horseradish peroxidase, and an auxiliary preparation. The usage method of the kit includes the following steps S1: coating the enzyme-labeled plate with a CHO HCP antibody; S2: adding a sample to be detected and a standard solution to the coated enzyme-labeled plate, incubating and washing; S3: adding biotin-labeled IgG to the enzyme-labeled plate washed in S2, incubating and washing; S4: adding streptavidin labeled with horseradish peroxidase to the enzyme-labeled plate washed in S3, incubating and washing; S5: adding a chromogenic solution to the enzyme-labeled plate washed in S4 for color development, incubating, adding a termination solution to terminate the reaction, and measuring the absorbance value at a wavelength of 450 nm. The kit makes up for the blank of a special HCP detection kit, and has high specificity and high detection precision for HCP detection.

The utility model discloses an azotometer for protein detection, and relates to the technical field of azotometers for protein detection. A connecting piece is fixedly connected to the side face of the digestion pipe, a telescopic rod is slidably connected to the interior of the fixing groove, a check block is fixedly connected to the bottom of a sliding rod, and a sliding block is connected to the outer portion of the sliding rod in a sleeving mode, and when the digestion pipe is clamped into the mounting groove, the check blocks fixedly connected to the two ends of the mounting groove are firstly clamped into the connecting groove; the telescopic rod shrinks towards the interior of the connecting piece due to the extrusion effect of the check block, when the top of the check block enters the interior of the connecting groove, the telescopic rod is clamped to the top of the check block under the reset effect of the telescopic spring, the telescopic rod continues to slide upwards outside the sliding block, and a protruding block at the bottom of the sliding block is driven downwards to be embedded into a clamping groove; at the moment, the telescopic rod can slide outside the sliding block and the stop block, and the digestion pipe is separated from the interior of the mounting groove, so that the digestion pipe is more convenient to disassemble and assemble.

The present invention provides a protein detection method and detection preparation, which realizes efficient enrichment of target protein through mesoporous silica-graphene oxide composite nanoporous material, completes specific immune capture in combination with magnetic nanoparticles modified with targeted antibodies, and realizes single molecule separation using a microfluidic chip of a three-dimensional hydrogel antibody array. Signal amplification adopts enzyme-labeled secondary antibody-gold nanorod conjugate, enhances the fluorescence signal through the plasma resonance effect, and corrects the background noise in combination with a convolutional neural network algorithm, which significantly improves the detection reliability; experiments show that the detection limit of this method is as low as 0.12pg / mL, the linear range covers 0.5-100,000pg / mL, the single cell detection error is ±7.8%, the batch repeatability (CV=4.2%), the nonspecific adsorption rate is only 1.1%, and the detection time is shortened to 1.2 hours. The detection method of the present invention is suitable for ultra-sensitive detection of tumor markers, especially in the in situ analysis of CTCs with clinical application potential.