631 results about "Protein detection" patented technology

A centrifugal force-based microfluidic device for the detection of a target biomolecule and a microfluidic system including the same are provided. The device includes a body of revolution; a microfluidic structure disposed in the body of revolution including chambers, channels connecting the chambers, and valves disposed in the channels to control fluid flow, the microfluidic structures transmitting fluid using centrifugal force due to rotation of the body of revolution; and beads disposed in the microfluidic structures, the beads having capture probes on the surfaces thereof which are selectively bonded with target protein; and a detection probe disposed in the microfluidic structures and selectively bonded to the target protein, and which includes a material required to express an optical signal, wherein the microfluidic structure mixes the beads, biological samples, and the detection probe to react and washes and separates the beads after the reaction.

The invention provides a multi-bead assay system for a protein based assay comprising at least two different beads. The first bead comprises protein and a protein stabilization matrix. The first bead forms a first solution when dissolved in liquid, and the first solution permits a first activity level for the assay. The second bead comprises a potentiation bead matrix that when dissolved in the first solution forms a second solution that potentiates the protein based assay to achieve a second activity level that is higher than the first activity level.

Multiplexed lateral flow assays, related methods, and devices are disclosed which are capable of simultaneously detecting multiple analytes. The assays are preferably immunoassays and can be multiplexed spatially, spectrally, and both spatially and spectrally. Multiplexed assays are disclosed employing quantum dots for applications including the detection of human proteins and the monitoring of microorganisms relevant to water contamination. The multiplexed assays can employ one or more species of Surface Enhanced Raman Scattering nanoparticles, with one or more species having a unique Raman shift spectrum. The invention is widely adaptable to a variety of analytes such as biowarfare agents, human clinical markers, and other substances.

A protein detecting device is disclosed. The device includes a reagent reservoir that is squeezable. The reagent reservoir has an end portion with a with a tubular member extending therefrom. This tubular member is hollow and in communication with the reservoir. The tubular member has an open end opposite the reservoir and has a cone shaped gasket in its open end. The cone shaped gasket has its base connected to an elongated member extending from the open end of the tubular member. The elongated member has a micro-brush at its end.

A centrifugal force-based microfluidic device for the detection of a target biomolecule and a microfluidic system including the same are provided. The device includes a body of revolution; a microfluidic structure disposed in the body of revolution including chambers, channels connecting the chambers, and valves disposed in the channels to control fluid flow, the microfluidic structures transmitting fluid using centrifugal force due to rotation of the body of revolution; and beads disposed in the microfluidic structures, the beads having capture probes on the surfaces thereof which are selectively bonded with target protein; and a detection probe disposed in the microfluidic structures and selectively bonded to the target protein, and which includes a material required to express an optical signal, wherein the microfluidic structure mixes the beads, biological samples, and the detection probe to react and washes and separates the beads after the reaction.

The invention provides a probe based on a G-quadruplex-chlorine heme DNA enzyme and a method for detecting protein. The method for detecting the protein facilitates detecting operation and is low in cost and free of marking. The probe has the advantages of being high in sensitivity and low in background noise. When the protein is detected, the detecting operation is easy, consumed time is short, cost is low, and the problems that a method for detecting the protein in the prior art is long in consumed time, high in cost and complex in detecting probe manufacturing are solved.

The present invention provides fluorogenic probes and corresponding fluorescent compounds, methods of using the probes, compounds and kits that include the probes.

The invention relates to a nanotechnology-based trace protein detection method, which combines enzyme-linked immunosorbent assay technology, tyramine signal amplification technology and the aggregation phenomenon of gold nanoparticles modified by different biological molecules, so an experimental method used for detecting trace proteins such as prostate specific antigen (PSA) and the like is established. The method comprises the following steps of: fixing an antibody aiming at the protein to be detected (such as the PSA) on the surface of a substrate; incubating another antibody with horseradish peroxidase (HRP) activity of the protein to be detected (such as the PSA) after capturing the protein to be detected in a sample, wherein the HRP catalyzes biotin-tyramide to generate biotin deposition under certain conditions; further amplifying a signal by using the aggregation phenomenon of the gold nanoparticles modified by biotin-labeled DNA and the gold nanoparticles modified by streptavidin; performing silver staining; and performing data analysis on an experimental result by using software. The method has the advantages of extremely low detection limit, wider detection range, capacity of detecting the antigen in a rabbit serum with complex compositions, and important application prospect.

The present embodiment describes a miniature, microfluidic, absorption-based sensor to detect proteins at sensitivities comparable to LIF but without the need for tagging. This instrument utilizes fiber-based evanescent-field cavity-ringdown spectroscopy, in combination with faceted prism microchannels. The combination of these techniques will increase the effective absorption path length by a factor of 10³ to 10⁴ (to ˜1-m), thereby providing unprecedented sensitivity using direct absorption. The coupling of high-sensitivity absorption with high-performance microfluidic separation will enable real-time sensing of biological agents in aqueous samples (including aerosol collector fluids) and will provide a general method with spectral fingerprint capability for detecting specific bio-agents.

The invention discloses a massive data-oriented recursive blocking information transfer clustering and indexing method. By using the method, the accurate and fast clustering of various massive data can be realized, and the method is suitable for an index structure of the query and the updating. Users can deal with massive and unordered data and can carry out fast clustering and indexing, thus being beneficial to the later query, search, maintenance and updating. The invention can be applied to the fast clustering and indexing of massive internet-oriented texts, images, videos, frequency, and the like, and can also be applied to similarity comparison of massive biological gene sequence and homologous protein detection. The invention also discloses a proximate and fast clustering method of massive data. The clustering method of the invention can ensure that the speed of the clustering can be increased exponentially under the circumstance of little loss of clustering effect and can be better beneficial to the clustering, inserting and updating of data outside a training set, therefore, the method can generally applied to the fast clustering and indexing of various complex massive data.

The present invention provides, for example, a target detecting device comprising a target capturer, means for releasing the target capturer, light irradiating means and light detecting means, the target capturer at least partially containing a region interactive with an electrically conductive member, being capable of capturing a target, and being capable of emitting light upon irradiation with light in the case of not interacting with the electrically conductive member, the means for releasing the target capturer serving to release the target capturer from the electrically conductive member by ceasing the interaction between the target capturer and the electrically conductive member, the light irradiating means serving to apply light to the electrically conductive member, and the light detecting means serving to detect light emitted by the target capturer upon irradiation of light applied by the light irradiating means. It also provides a target capturer comprising an interacting section, a capturing section and a light emitting section, the interacting section at least partially containing a region interactive with an electrically conductive member, the capturing section capable of capturing a target, and the light emitting section capable of emitting light upon irradiation with light when the region in the interacting section does not interact with the electrically conductive member.

The present invention relates to an isolated nucleic acid molecule comprising (a) a first nucleotide sequence encoding a protein that detects the presence, amount or both of a pathogenic microorganism by forming a complex with a quorum sensing molecule produced by said pathogenic microorganism, (b) one or more second nucleotide sequence said one or more second nucleotide sequence being under control of a promoter that is induced by the complex of the protein encoded by the first nucleotide sequence and the quorum sensing molecule produced by said pathogenic microorganism and encoding (i) an antimicrobial peptide, wherein the antimicrobial peptide is effective against the pathogenic microorganism detected by the protein encoded by the first nucleotide sequence; and/or an antibiofilm enzyme wherein the antibiofilm enzyme is effective against the pathogenic microorganism detected by the protein encoded by the first nucleotide sequence; and (c) optionally a third nucleotide sequence encoding a protein that controls the motility of the host organism, wherein the protein that controls the motility of the host organism directs the motility of the host organism towards said pathogenic microorganism. A recombinant microorganism comprising the isolated nucleic acid molecule and a method of sensing and killing pathogenic microorganisms is also described.

The present invention relates to a novel RNA picornavirus that is called Seneca Valley virus (“SVV”). The invention provides isolated SVV nucleic acids and proteins encoded by these nucleic acids. Further, the invention provides antibodies that are raised against the SVV proteins. Because SVV has the ability to selectively kill some types of tumors, the invention provides methods of using SVV and SVV polypeptides to treat cancer. Because SVV specifically targets certain tumors, the invention provides methods of using SVV nucleic acids and proteins to detect cancer. Additionally, due to the information provided by the tumor-specific mechanisms of SVV, the invention provides methods of making new oncolytic virus derivatives and of altering viruses to have tumor-specific tropisms.

The invention relates to a preparation method and application of an electrochemical immunosensor for a biomarker, namely a recombinant proprotein convertase subtilisin kexin type 9 (pcsk9) protein for predicting and diagnosing cardiovascular diseases, and belongs to the technical field of electrochemical detection. The preparation method is characterized by comprising the following steps: firstly, performing nitrogen doping on a graphene nanobelt (n-gnrs), performing amination on a fullerene-palladium-platinum nanoparticle (n-C60/pdpt) composite material and a staphylococcus aureus a protein (spa), and performing layer-by-layer self-assembling so as to immobilize a pcsk9 antibody (ab1); mixing the synthesized nanoparticle-poly-methylene blue (pt-pmb) with the pcsk9 antibody (ab2), and preparing a nano beacon, thereby obtaining the electrochemical immunosensor for pcsk9 protein detection. The electrochemical immunosensor has the advantages of being high in sensitivity, good in specificity and rapid and convenient in detection. A novel method for pcsk9 protein detection is provided, and useful information is provided for clinical prediction and diagnosis on cardiovascular diseases.

The invention discloses a di-pyrene modified perylene bisimide derivative fluorescent probe and a synthesis method and application thereof, and belongs to the technical field of protein detection. The preparation of the fluorescent probe comprises the following steps that in a protective atmosphere, 1-methyl-2-pyrrolidinone solution of a pyrene sulfonyl derivative is added into a 1-methyl-2-pyrrolidinone solution of perylene; then, anhydrous zinc acetate is added to obtain a mixed solution; next, the mixed solution is stirred; heating reflux is performed for 10 to 15h at 150 to 200 DEG C; after the reaction liquid obtained after the reflux is cooled to the room temperature, filtering is performed to obtain filter liquid; the filter liquid is subjected to acid washing; precipitates occur; suction filtration is performed; filter cake is obtained; the filter cake is washed and dried to obtain the di-pyrene modified perylene bisimide derivative fluorescent probe. The fluorescent probe provided by the invention has the advantages that the chemical stability is high; the response speed is high; the sensitivity is high; the discrimination performance is good; the sample consumption quantity is small; the data collection is simple, and the like. The instant and on-line detection of the protein can be realized. The fluorescent probe provided by the invention can be used for respectively realizing the distinguished detection of various proteins.

This invention relates generally to the field of glycated protein detection. In particular, the invention provides chimeric proteins, nucleic acids encoding the chimeric proteins, methods and kits for assaying for a glycated protein in a sample, using inter alia, an amadoriase.

The invention discloses a construction method of an impedance type electrochemical sensor based on a molecularly imprinted polymer with a magnetic surface and an application of the sensor in protein detection, and belongs to the technical field of electrochemical sensing. The construction method comprises the following steps: synthesizing the molecularly imprinted polymer on the surface of a magnetic nano particle by taking hemoglobin as a template molecule and dopamine as a monomer and by using the self polymerization performance and the good adhesive capacity of the dopamine; eluting the hemoglobin, so as to prepare the molecularly imprinted polymer with the magnetic surface, wherein the molecularly imprinted polymer with the magnetic surface has the good identification effect on the hemoglobin; and fixing the polymer on the surface of a magnetic glassy carbon electrode under the action of a magnetic field, so as to construct a hemoglobin sensing interface based on the molecularly imprinted polymer with the magnetic surface, wherein the hemoglobin sensing interface based on the molecularly imprinted polymer with the magnetic surface is used for hemoglobin detection. The constructed electrochemical sensor based on the molecularly imprinted polymer with the magnetic surface is simple in method, high in hemoglobin detection sensitivity and good in selectivity.

The invention discloses a novel coronavirus SARS-CoV-2 S protein detection method. SARS-CoV-2 virus depends on spike protein on the surface to be combined with angiotensin converting enzyme 2 on the surface of a cell so as to enter the cell; according to the method, raw materials commonly used in chemiluminescence immunoassay and ACE2 are easy to purchase from the market, the biotin labeling and the alkaline phosphatase labeling are rapid, the reagent for SARS-CoV-2 S protein detection can be rapidly prepared, and the positive coincidence rate, the negative coincidence rate and the total coincidence rate are high compared with the SARS-CoV-2 fluorescence quantitative PCR result.

A cavity electrode structure, which is provided with a pair of opposing electrodes having a precisely formed narrow gap, and a sensor and a protein detection device, in which the cavity electrode structure is used, are provided. The cavity electrode structure comprises a first electrode, an insulating layer located on this first electrode and having a through hole that partially exposes the first electrode, and a second electrode opposed to the exposed surface of the first electrode by protruding towards the inside of the through hole of the insulating layer and provided with an opening that leads to the through hole of the insulating layer, the structure having a cavity that is formed by the exposed surface of the first electrode, the inner walls of the through hole of the insulating layer, and the surface of the second electrode that opposes the first electrode. The sensor comprises an electrically conductive bridging member of which one end is fixed to the exposed surface of the first electrode of the aforementioned cavity electrode structure, while the other end is fixed to the opposing surface of the second electrode, and which has a site that specifically binds to a target protein to be detected. The protein detection device uses a bridging member provided with a site that specifically binds to a target protein to be detected.

Disclosed in the present invention are a mouse monoclonal antibody for antagonizing and inhibiting the binding of a programmed death-1 (PD-1) to the ligand thereof, and the heavy chain variable region and light chain variable region amino acid sequences thereof. Also disclosed in the present invention is a DNA molecule nucleotide sequence encoding the heavy chain variable region and light chain variable region of the antibody. Also disclosed in the present invention are a method for preparing a human-mouse chimeric antibody of the antibody and derivatives thereof, and the use thereof in PD-1 protein detection.