274 results about "Protein detection" patented technology

The present invention belongs to the field of biological detection technology, specifically relating to a monoclonal antibody combination for detecting HPV18 E7 protein and its application. This combination consists of monoclonal antibodies 5F8 and 6G6, wherein the heavy and light chain variable regions of 5F8 and 6G6, respectively, contain specific complementarity-determining region sequences (as shown in SEQ ID NOs. 1 to 12), conferring high specificity for HPV18 E7 protein. Verification using a double-antibody sandwich ELISA and a biotin-avidin amplification system demonstrated that this antibody combination specifically recognizes HPV18 E7 protein, exhibits no cross-reactivity with other high-risk HPV oncoproteins, and exhibits a detection sensitivity of 1 ng / mL. This combination can be used to construct detection systems such as ELISA kits and immunochromatographic test strips, suitable for rapid screening of HPV18 E7 protein in cervical exfoliated cell samples.

The invention belongs to the technical field of biological detection, and relates to an antibody composition aiming at pTau217 protein detection and application thereof, and the antibody composition aiming at pTau217 protein detection comprises a target pTau217 protein antibody and a target Tau protein C-terminal antibody. Meanwhile, based on the antibody composition aiming at pTau217 protein detection, the invention further provides a kit for early diagnosis of the Alzheimer's disease. The invention provides an antibody composition aiming at pTau217 protein detection, which is stronger in detection specificity and higher in sensitivity, and an application of the antibody composition.

The invention is applicable to the technical field of C-reactive protein detection, and provides an anti-C-reactive protein monoclonal antibody idiotype antibody, a preparation method and application, and the anti-C-reactive protein monoclonal antibody idiotype antibody is secreted by a hybridoma cell strain; the hybridoma cell strain is a 7C4 hybridoma cell strain; the preparation method of the anti-C-reactive protein monoclonal antibody idiotype antibody comprises the following steps: S1, obtaining hybridoma cells: preparing an anti-C-reactive protein monoclonal antibody Fab fragment, then carrying out animal immunization, then carrying out cell fusion, and finally screening and cloning the hybridoma cells; and S2, preparing and screening an anti-C reactive protein monoclonal antibody idiotype antibody. The anti-C-reactive-protein monoclonal antibody idiotype antibody is used for replacing C-reactive protein to serve as a labeled antibody, the labeling process is simple, the labeling efficiency is high, and meanwhile the situation that a final product is affected due to instability of the C-reactive protein is avoided. Experimental results show that the detection method is good in specificity and wide in linear range.

The invention belongs to the technical field of immunochemistry, and particularly relates to an anti-PR recombinant rabbit monoclonal antibody and application thereof.The anti-PR recombinant rabbit monoclonal antibody comprises a heavy chain variable region and a light chain variable region, and the amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 4; the amino acid sequence of the light chain variable region is as shown in SEQ ID NO: 5. The invention also relates to a nucleotide sequence for coding the anti-PR recombinant rabbit monoclonal antibody, a recombinant plasmid or an expression vector, a preparation method, application of the anti-PR recombinant rabbit monoclonal antibody in a PR protein detection method or device, and the like. The PR recombinant rabbit monoclonal antibody disclosed by the invention has the characteristics of good specificity, strong positive signal and the like, so that scoring is easier in IHC staining, and cancer detection and distinguishing are more accurate.

The present disclosure relates to proteomics, including the detection of immune cell target proteins as examples that the disclosed methods can be used for protein detection. Methods, compositions, and systems are described for identifying the presence of proteins, including detecting immune cell proteins from a sample using a combination of multiplex immuno-PCR with antibodies conjugated to oligonucleotides comprising an antibody-specific barcode flanked by PCR priming sites; detection of barcoded amplicons obtained from immuno-PCR with encoded padlock probes comprising codes specific for the barcodes / target protein; rolling circle amplification (RCA) of the circularized padlock probes; and determination of the sequence of the codes amplified by RCA by next generation sequencing or using fluorescently labeled hybridization probes.

The invention belongs to the technical field of bioengineering, and particularly relates to a nucleic acid aptamer specifically bound with MMLV, and the nucleotide sequence of the nucleic acid aptamer is as shown in SEQ ID NO.1 or SEQ ID NO.2; the nucleic acid aptamer capable of being specifically bound with MMLV is obtained through screening on the basis of the SELEX technology, has high affinity for MMLV protein, can be applied to MMLV protein enrichment reagents, MMLV protein separation reagents, MMLV protein detection reagents, test paper and biosensors, and facilitates identification and activity research of the MMLV protein.

The invention discloses a viral ARDS prognosis evaluation system based on protein joint detection, and the system comprises a sample processing module which is used for carrying out serum standardization pretreatment; the protein detection module is used for performing iBAQ quantification of IL6ST / FOXO3 / TLR7 based on DIA mass spectrometry, defining a targeted therapy threshold value and realizing targeted quantification of the core protein; and the intelligent analysis module is used for realizing cross-age risk layering based on a multivariable logic regression model. B cell function states are reflected by combining serum IL6ST / FOXO3 / TLR7 protein expression levels, a multivariable logistic regression model is constructed, and unified risk stratification of patients of all ages from children to the elderly is achieved; by locking an IL6ST / FOXO3 / TLR7 pathway, a targeted therapy threshold is defined to directly guide targeted therapy, and immune intervention is facilitated.

The invention provides a protein and metal ion binding site identification method, device and equipment, and belongs to the field of protein detection.The method comprises the steps that feature extraction is conducted on known metal ion binding protein, and multiple sample evolution information features are obtained; the method comprises the following steps: for an unknown protein sequence, determining candidate distant homologous metal ion binding proteins through multi-sequence comparison and cosine similarity screening, and further constructing a training set; a composite framework in which a bidirectional long-short-term memory network and a full-connection neural network are connected in series is adopted, input features comprise evolutionary information features and physicochemical attribute features, and probability values of binding sites of different metal ions are output; training the composite framework through the training set to obtain a site prediction model; and predicting a binding site of an unknown protein sequence by using the prediction model. The stable and efficient prediction on the metal ion binding site is realized.

The invention belongs to the technical field of biological detection, and relates to an antibody composition aiming at human pS129-alpha-Syn protein detection and application thereof, and the antibody composition comprises a targeted pS129-alpha-Syn protein phosphorylation antibody and a targeted human alpha-synuclein N-terminal antibody. Meanwhile, based on the antibody composition for detecting the human pS129-alpha-Syn protein, the invention further provides a kit for early diagnosis of the Parkinson's disease. The invention provides an antibody composition aiming at human pS129-alpha-Syn protein detection and application of the antibody composition. The antibody composition is higher in detection specificity and sensitivity.

The present disclosure relates to an azide-modified fluorescent π-conjugated polymer and a DNA-modified π-conjugated fluorescent nanoparticle-based protein detection imaging method capable of signal rapid amplification and high throughput. The present disclosure prepares oligonucleotide-modified fluorescent π-conjugated polymers (OCPNs) based on the azide-modified fluorescent π-conjugated polymer. The OCPNs have ultrahigh fluorescence luminance and extremely high detection sensitivity, and can complete detection and imaging of targets in different abundances without the need of an additional fluorescence signal amplification step. In addition, a rapid and specific targeting effect can be achieved with an extremely small dosage and an entire staining and imaging process can be shortened, so that the OCPNs achieve an extremely high imaging efficiency during multi-color imaging and thus exhibit great application prospects.

The invention belongs to the technical field of protein calibration, and particularly relates to an enhanced protein-free confining liquid for protein detection, which comprises the following components: trihydroxymethyl aminomethane, sodium chloride, Tween-20, polyvinyl alcohol, trehalose, EDTA (Ethylene Diamine Tetraacetic Acid) and a polyoxyethylene-polyoxypropylene block copolymer, and sodium chloride and trehalose are matched to provide ions and small molecular compounds to form a stable closed environment. Wherein the trehalose assists in filling and covering small pores of PVDF, the sodium chloride provides ion balance, the EDTA-Na is used for precipitating metal ions, and the Tween-20 assists in repairing the PVDF film. According to the invention, the polyvinyl alcohol and the polyoxyethylene-polyoxypropylene block copolymer are mixed for use, so that non-specific sites on the membrane are blocked while impurity proteins are prevented from being introduced, the sealing effect is achieved, and the problem of false positive or non-specific binding easily caused by the traditional sealing liquid is effectively solved.

The invention belongs to the technical field of biological detection, and particularly relates to a specific monoclonal antibody for AKK bacterium active protein P9, a detection kit and application of the specific monoclonal antibody. The antibody pair is 7G4 and 8B2, the amino acid sequence of a heavy chain variable region of the 7G4 is SEQ ID NO.2, and the amino acid sequence of a light chain variable region of the 7G4 is SEQ ID NO.4; the amino acid sequence of the 8B2 heavy chain variable region is SEQ ID NO.6, and the amino acid sequence of the 8B2 light chain variable region is SEQ ID NO.8. The antibody pair has strong specificity and high affinity, and can accurately recognize different antigen epitopes of the P9 protein. A colloidal gold detection and double-antibody sandwich ELISA detection system constructed based on the kit adapts to a complex matrix and various process treatment samples, is stable and reliable in detection, has qualitative and quantitative capabilities, can be used for scenes of P9 protein detection, fermentation monitoring, quality control and the like, and solves the problems of insufficient specificity, poor adaptability and the like in the prior art.

The invention discloses a protein quantitative detection method which comprises the following steps: respectively constructing a capture probe for capturing antibody coupling magnetic beads and oligonucleotides and a detection probe for detecting antibody coupling oligonucleotides through a biorthogonal chemical coupling method; then, target protein is captured by using a biorthogonal chemical coupling and magnetic bead sandwich method comprising the steps of capture probe combination, closing, washing, detection probe combination and the like, and accurate quantification of low-abundance protein in a complex sample is realized by combining a subsequent digital PCR (Polymerase Chain Reaction) technology.

The application discloses a preparation method of a VP@UiO-66-NH2 / Ag NPs composite SERS chip and application thereof in A beta protein detection, and is characterized by comprising the following steps: synthesizing a VP-graphite paper base by a chemical vapor transport method on a surface of a graphite paper to obtain a VP-graphite paper base; synthesizing a UiO-66-NH2 layer on the surface of the VP-graphite paper base by a solvothermal method to obtain a VP@UiO-66-NH2 composite base; and finally, placing the composite base in an ion sputtering instrument, uniformly sputtering silver nanoparticles on the surface of the composite base, taking out the composite base, and obtaining the VP@UiO-66-NH2 / Ag NPs composite SERS chip, wherein the VP@UiO-66-NH2 / Ag NPs composite SERS chip has the advantages of high sensitivity, good stability and uniformity, controllable preparation process, low cost, application in Alzheimer's disease biomarker detection, high specificity in identification and quantitative analysis of A beta 40 and A beta 42 proteins, fast response and good stability.

The invention relates generally to peptide biomarkers with specific ionization characteristics to directly quantify one or more target HPPD proteins in biological samples, including crop plant samples, by liquid chromatography coupled tandem mass spectrometry multiple reaction monitoring (MRM). The peptide biomarkers in combination with MRM-based methods may be used to quantify a single target protein or multiple target proteins within a crop plant, such as maize, utilizing selected peptide biomarkers either alone or in combination. The present disclosure allows for broad based, reliable quantitation in different biological matrices, including plant matrices. Also provided are different peptide biomarker combinations that can be used to perform the methods of the invention.

The utility model discloses an azotometer for protein detection, and relates to the technical field of azotometers for protein detection. A connecting piece is fixedly connected to the side face of the digestion pipe, a telescopic rod is slidably connected to the interior of the fixing groove, a check block is fixedly connected to the bottom of a sliding rod, and a sliding block is connected to the outer portion of the sliding rod in a sleeving mode, and when the digestion pipe is clamped into the mounting groove, the check blocks fixedly connected to the two ends of the mounting groove are firstly clamped into the connecting groove; the telescopic rod shrinks towards the interior of the connecting piece due to the extrusion effect of the check block, when the top of the check block enters the interior of the connecting groove, the telescopic rod is clamped to the top of the check block under the reset effect of the telescopic spring, the telescopic rod continues to slide upwards outside the sliding block, and a protruding block at the bottom of the sliding block is driven downwards to be embedded into a clamping groove; at the moment, the telescopic rod can slide outside the sliding block and the stop block, and the digestion pipe is separated from the interior of the mounting groove, so that the digestion pipe is more convenient to disassemble and assemble.

The present invention provides a protein detection method and detection preparation, which realizes efficient enrichment of target protein through mesoporous silica-graphene oxide composite nanoporous material, completes specific immune capture in combination with magnetic nanoparticles modified with targeted antibodies, and realizes single molecule separation using a microfluidic chip of a three-dimensional hydrogel antibody array. Signal amplification adopts enzyme-labeled secondary antibody-gold nanorod conjugate, enhances the fluorescence signal through the plasma resonance effect, and corrects the background noise in combination with a convolutional neural network algorithm, which significantly improves the detection reliability; experiments show that the detection limit of this method is as low as 0.12pg / mL, the linear range covers 0.5-100,000pg / mL, the single cell detection error is ±7.8%, the batch repeatability (CV=4.2%), the nonspecific adsorption rate is only 1.1%, and the detection time is shortened to 1.2 hours. The detection method of the present invention is suitable for ultra-sensitive detection of tumor markers, especially in the in situ analysis of CTCs with clinical application potential.

The present application discloses a myocardial N69 protein protective solution and a myocardial N69 protein detection kit. The present application designs a myocardial N69 protein protective solution, the ratio of which includes: 0.12% to 0.48% by mass of Tris-HCl buffer, 1% to 5% by mass of BSA or 0.1% to 0.5% by mass of caseinate sodium, 0.2% to 2% by mass of disodium EDTA, 5% to 20% by mass of trehalose, 0.02% to 0.06% by volume of a surfactant, and 0.08% to 0.12% by volume of a preservative, ProClin300; it also includes a protease inhibitor and a reducing agent. The present application also designs a myocardial N69 protein detection kit. The present application can effectively and long-term preserve N69 protein calibrants and can quickly and accurately detect N69 protein.

The invention provides a method for improving protein detection accuracy by using a coomassie brilliant blue ratio-type probe and application of the method. Absorbance at two peaks of 465nm and 595nm of bovine serum albumin-coomassie brilliant blue solutions with different concentrations are detected, and the protein detection accuracy is improved by using the ratio of absorbance values from two wavelengths and different absorption peaks. Self-calibration ratio signals are obtained, a linear relation between the peak value ratio of the two peaks and the BSA standard concentration is constructed, and quantitative detection is achieved. The detection method has the advantages of simplicity, rapidness, low cost and less interference, and is suitable for modernization measurement. The method can be used for protein detection, especially for protein detection in milk powder.

The invention discloses a method for detecting the content of hepatocyte growth factors of mesenchymal stem cells, and belongs to the technical field of protein detection. The method comprises the steps that an MSC cell suspension with the known concentration is taken, the MSC cell suspension and a cell lysis solution are mixed according to the set volume ratio and matched with a direct lysis technology to prepare a to-be-detected sample, and the lysis solution is prepared from disodium hydrogen phosphate, TritonX-100, NP-40 and SDS with the specific content. The lysate does not influence the combination of the antigen and the antibody, so that the content of the HGF of the mesenchymal stem cells is detected by adopting ELISA (enzyme-linked immunosorbent assay) and can be used as an important biological function index for evaluating MSC (mesenchymal stem cells) immunoregulation, tissue repair and cell apoptosis inhibition. The method is simple, convenient and efficient, compared with a traditional repeated freezing and thawing sample preparation method, the sample preparation period is greatly shortened, the sample cracking efficiency is improved, and a better sample preparation method is provided for detecting the protein content of the mesenchymal stem cells.

The invention discloses a detection method of inflammatory marker protein. According to the method, the micro-fluidic chip with a sample loading area, a buffer treatment area, a sample channel and a detection area is prepared on filter paper through wax printing; chitosan is preset in the sample loading area and is used for treating a sample, and bromothymol blue is fixed in the detection area and is used as a pH indicator. During detection, a sample is driven by a phosphate buffer solution with the pH value of 6.0 to flow to a detection area through capillary action, the C-reactive protein and the indicator are subjected to non-specific binding, so that the color of the detection area is changed from yellow to green, the length of a green developing signal is in direct proportion to the concentration of the CRP, and quantitative detection can be realized by measuring the length. The method does not need an antibody, is low in cost, is simple and convenient to operate, can complete detection within 15 minutes, has the detection limit of 1.56 ng / ml and the linear range of 5-25 ng / ml, and is suitable for rapid screening and field detection of basic medical institutions.

1. The name of the design product: storage (urine protein). 2. The use of the design product: for urine protein detection sample storage. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The invention discloses a colorimetric detection method for cMyBP-C protein based on nano-enzyme activity, preparation of a bismuth selenide / nano-gold composite material and application of the bismuth selenide / nano-gold composite material in protein detection. The method comprises the following steps: performing ultrasonic stripping on a bismuth selenide block to obtain a few-layer bismuth selenide dispersion liquid; and then sequentially adding a sodium borohydride aqueous solution and a chloroauric acid aqueous solution under a stirring condition, and carrying out in-situ reduction to form gold nanoparticles loaded on the surface of bismuth selenide. The composite material has the capability of catalyzing reduction of p-nitrophenol into p-aminophenol, and the color of the solution is changed from yellow to colorless. After the cMyBP-C antibody is modified on the surface of the composite material, the catalytic activity is inhibited; after a to-be-detected sample is added, the antibody and the protein are specifically combined to cause dissociation, and the catalytic activity is recovered. The cMyBP-C protein is quantitatively detected by observing the color change of the solution or monitoring the reduction of the ultraviolet absorbance at 400 nm.

The invention relates to a kit for detecting Tau-181 protein and application, and relates to the technical field of biological detection.The kit comprises a first antibody coupled with a first component of DNAzyme with catalytic activity, a second antibody coupled with a second component of DNAzyme with catalytic activity, phi29DNA polymerase, dNTPs, an annular DNA template, an initiation primer, a fluorescent beacon probe and a reaction buffer solution; the Tau-181 protein detection technology based on double-antibody sandwich coupling DNAzyme is used for quantitatively detecting the content of the Tau-181 protein in a biological sample in vitro, and can be applied to the fields of neuroscience research and clinical diagnosis, especially in the processes of early diagnosis, disease monitoring and drug research and development of neurodegenerative diseases such as Alzheimer's disease. And the method has important significance in the aspects of detection and analysis of a disease-related biomarker Tau-181 protein and the like.

The application discloses a monoclonal antibody of a rice field eel Adam8a and a preparation method and application thereof, relates to the technical field of biological antibodies, and fills the research blank of the rice field eel Adam8a. The rice field eel Adam8a monoclonal antibody is high in specificity and titer, simple in preparation process, and controllable in cost, can be widely used for recognizing Adam8a homologous proteins in different fish ovary, and is suitable for being prepared into a fish ovary Adam8a protein detection kit, and is compared with the expression of the protein in different fish, so that the rice field eel Adam8a monoclonal antibody becomes a very convenient detection tool in the biological research of fish ovary development.

The invention relates to a synchronous detection method and system for small molecules and protein, and the synchronous detection method comprises the following steps: respectively preparing a small molecule detection antibody and a protein detection antibody marked by carboxyl fluorescent microspheres, and mixing the small molecule detection antibody and the protein detection antibody to obtain a mixed detection antibody; respectively preparing a microsphere-labeled capture micromolecule antigen and a protein antibody; preparing a microsphere array biochip with two detection areas; carrying out sample application on the microsphere-labeled captured micromolecule antigen and the protein antibody to the two detection areas; mixing a sample to be detected with the mixed detection antibody according to different proportions; slowly adding the sample mixed solution into two detection areas of the microsphere array biochip respectively; scanning and imaging the two detection areas of the microsphere array biochip respectively, identifying all fluorescent spot information in a fluorescent image, and obtaining the concentration of small molecules and proteins in a sample to be detected through a Poisson distribution principle or by establishing a standard curve. According to the invention, synchronous detection of small molecules and proteins can be realized.

The invention discloses a polyclonal antibody of panda Rbp7 protein as well as a preparation method and application thereof, and belongs to the technical field of antibodies. The first to nineteenth amino acid sequences of the panda Rbp7 protein are used as antigen polypeptides, and are specifically shown as SEQ ID No.1. After chemical synthesis or exogenous expression purification, a Balb / C mouse is immunized, and the polyclonal antibody is obtained through repeated immunization, serum titer detection and affinity chromatography purification. The titer of the antibody is not lower than 1 / 128000, the panda Rbp7 recombinant protein can be efficiently and specifically detected through WB and ELISA, the blank of a protein detection tool is filled, and reliable technical support is provided for research on the functional mechanism of the panda Rbp7 protein.

Provided is a protein detection method for detecting a protein contained in a biological tissue collected from a living body. The protein detection method comprises: a step for preparing a specimen sample by adding, to a sample containing a protein collected from a living body, antibody-carrying magnetic particles that bind to the protein, and mixing the antibody-carrying magnetic particles and the sample; a measurement step for measuring a magnetic signal corresponding to a magnetic field generated by the specimen sample by applying, to the specimen sample, a switching magnetic field in which the magnetic field direction reverses; and a detection step for detecting the protein contained in the specimen sample on the basis of the magnetic signal.

The utility model relates to the technical field of protein detection, and provides a protein pretreatment device which comprises a base, a digestion frame, an adding mechanism, a first driving part and a second driving part, the digestion frame is arranged on the base, and a plurality of digestion tube clamping holes are formed in the digestion frame; the adding mechanism is connected with the base, is positioned above the digestion frame, and is used for adding a protein detection solution into the digestion tube in the digestion tube clamping hole; the first driving part comprises a first driving end and a first fixing end, the first driving end is connected with the adding mechanism, and the first fixing end is connected with the base so as to drive the adding mechanism to move in the first direction; the second driving part comprises a second driving end and a second fixing end, the second driving end is connected with the adding mechanism, the second fixing end is connected with the base so as to drive the adding mechanism to move in the second direction, the first direction is perpendicular to the second direction, and the formed plane is parallel to the plane where the digestion tube clamping holes are located. Automatic operation is achieved, and manual operation is reduced.

The invention provides an analysis kit and a detection method of prostate small body leaked protein, and relates to the technical field of kits.The analysis kit comprises a kit body, a heating cavity is formed in the portion, adjacent to the lower portion of a storage groove, in the kit body, and an electric heating piece is fixedly installed on the top face of the inner end of the heating cavity; the microcontroller synchronously starts the failure judgment timing module, if the detection articles are not used within one hour, the module feeds back a signal to enable the microcontroller to control the micro motor to lock the box cover again, and the electric heating sheet in the heating cavity of the box body is started to forcibly discard the reagent, so that the detection error caused by the active failure reagent is prevented; according to the present invention, the accurate detection is ensured through the preparation before the detection and the reagent aging control, and the problems that the accuracy of the detection result is affected due to the lack of the temperature balance control mechanism and the reagent aging control function of the current prostate small body leakage protein detection kit so as to interfere the prostate disease clinical diagnosis and illness state evaluation are solved.