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Micro-fluidic chip for glycosylated hemoglobin immunodetection

A glycosylated hemoglobin, microfluidic chip technology, applied in biological testing, material inspection products, etc., can solve the problems of hindering the application and promotion of diabetes, complicated operation procedures, large sample consumption, etc., and achieve huge economic value and social value. The effect of simplifying the operation process and reducing the cost of use

Active Publication Date: 2013-09-25
ZHEJIANG PUSHKANG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the detection of glycosylated hemoglobin is mainly completed by large-scale biochemical analyzers or ion-exchange high-pressure liquid chromatography. Operating environment and professional technical operators, the above shortcomings have seriously hindered the application and promotion of instant and rapid detection of diabetes

Method used

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  • Micro-fluidic chip for glycosylated hemoglobin immunodetection
  • Micro-fluidic chip for glycosylated hemoglobin immunodetection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 A kind of glycosylated hemoglobin immunoreaction detection chip for single sample

[0019] figure 1 It is a schematic diagram of a glycosylated hemoglobin immune reaction detection chip for a single sample. Such as figure 1 As shown in a, the chip is composed of a sampling pool 1 , a reaction detection pool 3 , a waste liquid pool 4 , a pump valve interface 5 and a microfluidic channel 2 . Its three-dimensional composition is as figure 1 As shown in b, in order to ensure that the sample solution and reaction products in the microchannel and reaction cell inside the chip are not interfered by external visible light and detection light source, a black PMMA board is used to process the light shielding layer 7 to construct the microfluidic channel 2 and the reaction detection cell 3; The detection cell needs to be transparent to the detection light, so it is composed of a transparent cover plate 6 and a transparent bottom plate 8 . When in use, the required an...

Embodiment 2

[0020] Example 2 A glycosylated hemoglobin immunoreaction detection chip for multiple samples

[0021] figure 2 It is a schematic diagram of a glycosylated hemoglobin immunoreaction detection chip for multiple samples. The chip structure adopts the layout of repeating the smallest functional unit, using a common waste liquid pool 4' and a pump valve interface 5'. When in use, the required antigens or antibodies are pre-immobilized in the reaction pool 3', and each serum sample solution to be tested is sequentially added to the sample pool 1' during detection, and the microfluidic channel 2 passes through the microfluidic channel 2 under the action of a common external pump 'respectively enter the reaction pool 3' for immunoagglutination reaction, after the reaction is terminated, the photoelectric detector is turned on for turbidimetric detection. The photodetectors can be arrayed to obtain multiple detection results at one time, or a single detector can be moved to differe...

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Abstract

The invention provides a micro-fluidic chip for glycosylated hemoglobin immunodetection in serum, comprising a sample introduction pool, a micro-fluid channel, a reaction tank, a detection tank, a waste liquor tank and a pump valve interface, wherein the sample introduction pool, the micro-fluid channel, the water liquor tank and the pump valve interface are serially connected by the micro-liquid channel; antigens or antibodies necessary for protein detection are fixed in the reaction and detection tanks in advance, a serum sample solution to be detected successively flows in the reaction tank by virtue of the sample introduction pool and the micro-fluid channel by an externally connected pump valve system, completes antigen or antibody specific reaction in the reaction tank and agglomerates, and the reaction product is subjected to absorbancy analysis to obtain the immune agglutination reaction and detection of the sample to be detected. The chip is convenient for sample introduction, less in sample consumption, high in reaction efficiency and short in detection time.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips, and provides a microfluidic chip for immune detection of glycosylated hemoglobin in serum. Background technique [0002] Diabetes is a lifelong disease and has become a major health care problem in countries all over the world due to its high complications and high mortality. Clinically, diabetes is mainly determined by the detection of blood sugar parameters, which only represent the blood sugar level at the time of blood drawing, which has limitations for diagnosis. Medical research in recent years has proved that the concentration of glycosylated hemoglobin (HbA1c) in the blood is relatively stable, and its concentration can accurately reflect the blood sugar level in the last 2 to 3 months, which is convenient for doctors to diagnose diabetes early. At present, the detection of glycosylated hemoglobin is mainly completed by large-scale biochemical analyzers or ion-exchange high-pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/72
Inventor 余波张铁成叶嘉明何宏燕李利
Owner ZHEJIANG PUSHKANG BIOTECHNOLOGY CO LTD
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