58 results about "Immobilized proteins" patented technology

A method for the preparation of an immobilized protein ultrathin film reactor by immersing a solid support alternately into an aqueous solution of protein, and into an aqueous solution of polyion charged oppositely to said protein and preparing a structurally controlled ultrathin film of mono- or multi- protein layers on a said solid support with precision at molecular level. And a method for chemical reaction of a substrate by preparing an immobilized protein ultrathin film reactor composed of multiple layers of protein on a solid support by above mentioned method, and initiating a chemical change of the substrate molecules using obtained immobilized protein ultrathin film reactor.

The invention provides a preparation method of drug and protein sustained-release alginate hybrid gel. The preparation method comprises the steps of preparing calcium alginate hydrogel, then soaking the calcium alginate hydrogel in inorganic salt solutions including trisodium phosphate, diammonium hydrogen phosphate, sodium silicate, sodium carbonate, sodium oxalate and the like at certain concentration to swell for a certain period of time, and soaking the swelled calcium alginate hydrogel into an aqueous solution of drugs and proteins with calcium ions at certain concentration for crosslinking for a period of time, thus obtaining the drug and protein sustained-release alginate hybrid gel. The calcium alginate hydrogel disclosed by the invention can be in the shape of a membrane, a microballoon, a fiber and the like. The method disclosed by the invention is simple to operate and does not adopt any poisonous and harmful solvent; the activity of the immobilized protein is high; the materials are good in biocompatibility; and the drug and protein sustained-release alginate hybrid gel is good in slow release performance, and has good application prospect in the fields of tissue engineering, controlled release, medicine and health, and the like.

This invention relates to the purification of monoclonal antibodies from mammalian cell culture fluid utilizing sequential, orthogonal chromatography and filtration techniques resulting in material of high purity and quality that is suitable for human administration. The method involves capturing an IgG product using immobilized protein A affinity chromatography, followed by at least one ion exchange technique prior to adsorbing the IgG to hydroxyapatite and selectively eluting the product in a single isocratic step to achieve purification from impurities and simultaneously reducing multiple types of impurities including but not limited to IgG aggregates, residual protein A, non-IgG proteins, host cell proteins, viral particles, and DNA

The invention discloses a graphene (GR)-exfoliation hydrotalcite-like compound (ELDH) composite material immobilized protein modified electrode, a production method and an application thereof. The method comprises the following steps: compounding ELDH with negatively-charged exfoliation graphene oxide (GO) nanosheets through electrostatic attraction by using the characteristics of positive charges and large specific surface area of the ELDH, and reducing by using hydrazine hydrate to prepare a GR-ELDH hybrid; and sequentially immobilizing the GR-ELDH, ferrohemoglobin and chitosan on an ionic liquid modified carbon paste electrode through adopting a dispensing technology to produce the graphene-exfoliation hydrotalcite-like compound composite material immobilized protein modified electrode. The obtained modified electrode has monolayer GR nanosheet and ELDH synergistic effects, and the GR nanosheets increase the conductivity of the ELDH and inhibits the aggregation and accumulation of the ELDH; and the ELDH effectively inhibits the afresh stacking of the GR nanosheets, reduces the interlamellar contact resistance and improves the electron transfer rate of the above composite material, and a constructed CTS/GR-ELDH-Hb composite film-based third-generation trichloroacetic acid sensor has the advantages of low detection limit, wide detection range and small Michaelis constant.

This invention provides an immobilized protein bound to an immobilization carrier at a protein amino terminus via the sole α-amino group of the protein comprising an amino acid sequence containing neither lysine residues nor cysteine residues represented by the general formula S1-R1-R2, wherein: the sequences are oriented from the amino terminal side to the carboxy terminal side; the sequence of the S1 portion may be absent, but when the sequence of the S1 portion is present, the sequence of the S1 portion is a spacer sequence composed of amino acid residues other than lysine and cysteine residues; the sequence of the R1 portion is the sequence of a subject protein to be immobilized and contains neither lysine residues nor cysteine residues; and the sequence of the R2 portion may be absent, but when the sequence of the R2 portion is present, the sequence of the R2 portion is a spacer sequence composed of amino acid residues other than lysine and cysteine residues.

The invention discloses a method for purifying and directionally immobilizing histidine tag protein and application. According to the method disclosed by the invention, tannic acid, metal ions and a hydrophilic anti-protein adhesion polymer are self-assembled and modified on the surface of a carrier to obtain a material surface which is efficiently chelated with cobalt ions, so that one-step purification and immobilization of the histidine tag protein is realized; a ligand of trivalent cobalt has inertia and divalent cobalt ions are oxidized into trivalent cobalt ions so that the stability of the immobilized protein can be improved. The method disclosed by the invention has the advantages of simple process and low price; a separation and purification or immobilization operation process for the histidine tag protein of the method is simple and effective, so that the technology has a wide application prospect.

A method for purifying a protein using Protein A chromatography comprising a) absorbing the protein to Protein A immobilized on a solid support; b) removing contaminants by washing the immobilized Protein A containing the absorbed protein with a buffer comprising one or more chaotropic agents in combination with one or more hydrophobic modifiers and having a pH of at least 7.0; and c) eluting the protein from the Protein A immobilized on the solid support.

To provide a method for detecting a protein by immobilizing a protein on a protein array substrate at a high density with controlled orientation, irradiating the immobilized protein with ultraviolet light, visible light, or infrared light, and measuring the light not absorbed by the protein and further analyzing an interaction between the protein on the substrate and another protein and/or a compound other than proteins, a system used for the method, and to provide a protein array suitable for the system. A system including a protein array in which a protein is immobilized in aligned position on a light-transmissive substrate at a high density, a light-irradiating means, and a light-detecting means and being for detecting or analyzing a protein on the protein array and/or a compound which is other than proteins and interacts with the immobilized protein, wherein the protein array is irradiated with light by the light-irradiating means, and the light transmitted through the protein array is measured by the light-detecting means for detecting the light absorption of the protein on the protein array and/or the compound which is other than proteins and interacts with the immobilized protein.

The invention relates to the field of covalently attaching proteins to a substrate, particularly to methods of immobilizing proteins by posttranslationally modifying a cysteine residue of said protein through the addition of functional groups. The invention also relates to biological molecules used in such techniques, including proteins, and detection methods and kits that utilize such immobilized proteins, such as a microdevice or “protein chip”, a high-throughput screening device, and for the microscopy of proteins on a surface.

An immobilized protein catalyst is prepared by applying an adhesive to a polymeric support, applying a layer of a globular protein over the layer of adhesive, binding a crosslinking agent to the protein layer, and binding the protein catalyst by reaction with the crosslinking agent.

The invention discloses a method for rapidly preparing a chromatin immunoprecipitation sample. The method comprises the following steps: performing formaldehyde crosslinking on a cell to fix a complex of a protein and a nucleic acid, extracting the complex of the protein and the nucleic acid, and breaking a chromatin segment into small segments in an ultrasonic or enzymatic hydrolysis manner; suspending an antibody onto a solid-phase support, i.e. a magnetic bead, adding the obtained small segments onto the prepared magnetic bead, performing incubation to obtain a magnetic bead antigen antibody complex, washing an uncombined substance away after incubation is finished, and resuspending the magnetic bead antigen antibody complex to obtain a magnetic bead antigen antibody complex sample to be detected. The method is easy to operate; the prepared magnetic bead antigen antibody complex sample can be directly used for PCR detection or amplification and construction of a DNA library, so that the sample preparation time is obviously shortened, meanwhile, the shortcoming of weaker enrichment signals of certain proteins and DNAs caused by excessive elution steps is overcome, the detection sensitivity is greatly improved, and influence on subsequent PCR identification and DNA segment amplification is avoided.

The invention discloses an ionic liquid covalent-modified graphene-stripped hydrotalcite-like composite material fixed protein-modified electrode, and a preparation method and an application thereof in detection of trichloroacetic acid. The special solubility and high conductivity of an ionic liquid are utilized, the ionic liquid is introduced into the surface of a graphene-stripped hydrotalcite-like composite material through a covalent modification method, an ionic liquid covalent-modified graphene-stripped hydrotalcite-like hybrid is prepared, and the ionic liquid covalent-modified graphene-stripped hydrotalcite-like composite material fixed protein-modified electrode is prepared by a dropwise coating method. The obtained modified electrode plays the synergistic effect of the ionic liquid, graphene and stripped hydrotalcite-like flakes in improvement of hemoglobin direct electrochemistry and electrocatalytic properties, and the conductivity, dispersivity and biocompatibility of the modified electrode are improved. A third-generation trichloroacetic acid sensor based on the biological modified electrode is built and has the advantages of low detection limit, wide detection range and small Michaelis constant and the like.

The invention provides a method for quickly immobilizing proteins or amino-contained molecules on an aminated surface. Amino-contained molecules are firstly assembled on a solid-phase surface such asglass, silicon chip, gold and the like to form an aminated surface, a mixture of N-hydroxy-succinamide and 1-ethide-3-(3-dimethyllaminopropyl) carbodiimide is used for functionalizing the carboxyl ofa poly-carboxyl compound, the functionalized poly-carboxyl compound reacts with the aminated surface, the immobilization of the poly-carboxyl compound on the solid-phase surface is realized by the combination of the functionalized carboxyl and the amino, and the remaining functionalized carboxyl can react with protein molecules to be immobilized and other amino-containing molecules. The method canquickly introduce the functionalized carboxyl to the aminated surface, realize the immobilization of the proteins, simplify surface-modifying steps and shorten the time for immobilizing the proteins.The method has the advantages of simple operation and environment friendliness and is suitable for the covalent immobilization of the amino-contained molecules on the aminated surface.

The present invention relates to a novel parallel throughput system that permits simultaneous screening of compounds in different modules of the system. Each module comprises a support having at least one species of protein binding moiety either immobilized through a covalent bond with the support surface to form an immobilized protein binding moiety or non-covalently immobilized in a stationary phase such that the tertiary structure of the protein in either immobilized binding moiety permits specific binding to a molecule that is bound by said protein in said immobilized binding moiety, and at least one marker molecule associated with the protein binding moiety species.

The invention discloses a mesoporous bioactive glass/poly-decyl diacid glyceride composite support as well as a preparation method and an application thereof. The composite support of the invention uses a mesoporous bioactive glass porous support as a matrix and compounds poly-decyl diacid glyceride on the mesoporous bioactive glass porous support. Mechanical strength, degradation rate, drug release rate, cell behavior and the like of the composite support of the invention can be adjusted by pore wall thickness of the mesoporous bioactive glass matrix and thickness of the poly-decyl diacid glyceride coating. A PGS coating is utilized to successfully solve the problem of brittleness of an MBG support, so that mechanical strength of the composite support is adjustable in a load bearing range of a trabecular bone. According to the invention, adjustment to degradation rate and release rate of proteins immobilized in MBG mesopore can be realized and activities of the immobilized proteins free of influence from a moderate coating operation can be realized; and cell experiments prove that the PGS coating is capable of promoting cell adhesion and proliferation on surface of the support without affecting osteogenic activity of the MBG.

A compound for immobilizing proteins and a method for immobilizing the proteins are disclosed. The compound has a structure of formula I, which can covalently immobilize the proteins, especially sjGST-tagged proteins, by means of position selective covalent bonding. In the Formula I, R1 is C1-C12 alkylene, preferably C3-C6 alkylene; R2 is C1-C10 alkyl, preferably C1-C3 alkyl; R3 is H or CH(triplebond)C-(C0-C10) alkyl, preferably CH(triple bond)C-(C0-C2) alkyl, more preferably CH (triple bond) C-.

The invention discloses a method for removing recombinant protein endotoxin by a GEM, and relates to a method for removing endotoxin in an escherichia coli expression recombinant protein. After a protein-AcmA fusion protein recombination expression carrier is subjected to inducible expression, the GEM is used for carrying out purification and immobilization, and then, Triton X-114 is used for processing the immobilized protein to remove the endotoxin. By use of the method, endotoxin removal work is carried out at a low temperature of 4 DEG C, a situation that the activity of the protein is affected since a temperature is changed is avoided, and an endotoxin removal operation is simplified.

The invention discloses a method for immobilizing a protein on TiO2 and regulating and controlling cell affinity by utilizing ultraviolet irradiation and TiO2-protein products, and relates to the field of biological materials. According to embodiments provided by the invention, the method for immobilizing the protein on the TiO2 and regulating and controlling the cell affinity by utilizing the ultraviolet irradiation comprises the steps of placing a TiO2 material into a protein solution to make the protein adsorbed at the TiO2 surface, taking out the treated material, and performing washing with water to wash off an unadsorbed protein, so as to obtain a TiO2-protein sample; and performing dry or wet ultraviolet irradiation on the TiO2-protein sample to obtain the TiO2-protein products withdifferent cells affinity. The method for immobilizing the protein on the TiO2 and regulating and controlling the cell affinity by utilizing the ultraviolet irradiation provided by the invention can effectively regulate and control the cell affinity of the protein adsorbed at the TiO2 surface; and the obtained TiO2-protein products can meet the personalized needs of different biological material application fields on the biological properties of materials, and have important practical value.