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Compound for immobilizing proteins and method for immobilizing proteins

A compound and protein technology, applied in the field of immobilized proteins, can solve problems such as limited applications

Inactive Publication Date: 2019-09-10
PEKING UNIV SHENZHEN GRADUATE SCHOOL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] GST-tagged proteins have high substrate affinity, however, known GST-tag immobilization methods are still based on reversible GSH-GST binding, limiting their applications

Method used

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  • Compound for immobilizing proteins and method for immobilizing proteins
  • Compound for immobilizing proteins and method for immobilizing proteins
  • Compound for immobilizing proteins and method for immobilizing proteins

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Embodiment Construction

[0033] specific implementation plan

[0034] In the following examples, all the reagents used in the experiments were purchased from reagent companies and no further processing was performed. Dichloromethane was dried over calcium hydride to remove water, and tetrahydrofuran was dried over sodium block to remove water. All reactions were monitored by 0.25 mm thick TLC silica gel plates and UV light. The silica gel used in flash column chromatography is 200-300 mesh silica gel powder. The Bruker 400M and 500M NMR instruments were used for the collection of hydrogen and carbon spectra, with trimethylsilicon (δH 0.00 and δC 0.00) as a reference. The 19F spectrum adopts a Bruker 400 mega-nuclear magnetometer, and fluorotrichloromethane (δF 0.00) is used as a reference. The 32P spectrum adopts a Bruker 400 M NMR instrument, with 85% phosphoric acid (δP 0.00) as a reference. High-resolution mass spectrometry data were collected using a quadrupole time-of-flight tandem mass spect...

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Abstract

A compound for immobilizing proteins and a method for immobilizing the proteins are disclosed. The compound has a structure of formula I, which can covalently immobilize the proteins, especially sjGST-tagged proteins, by means of position selective covalent bonding. In the Formula I, R1 is C1-C12 alkylene, preferably C3-C6 alkylene; R2 is C1-C10 alkyl, preferably C1-C3 alkyl; R3 is H or CH(triplebond)C-(C0-C10) alkyl, preferably CH(triple bond)C-(C0-C2) alkyl, more preferably CH (triple bond) C-.

Description

technical field [0001] The present invention relates to a compound for immobilizing proteins and a method for immobilizing proteins. Background technique [0002] Immobilization of proteins to solid supports such as microarrays, chips, and beads is a powerful method for elucidating protein-protein or protein-ligand interactions and for probing novel protein functions in a high-throughput manner. Unlike the case of DNA microarrays, implementing a general method for protein immobilization presents challenges due to inherent difficulties in achieving selective and uniformly oriented distribution of proteins into solid surfaces. [0003] Conventional immobilization methods mainly rely on three mechanisms: physical attachment, covalent bonding, and bioaffinity immobilization. However, non-selective physical attachment or covalent bonding may block the active site or even denature the protein. Immobilization by co-expression of recombinant fusion tags and coupled proteins can ac...

Claims

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Application Information

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IPC IPC(8): C07F9/6561C07K17/02
CPCC07F9/6561C07K17/02
Inventor 潘峥婴王厦峰郭天林
Owner PEKING UNIV SHENZHEN GRADUATE SCHOOL
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