133 results about "Protein immobilization" patented technology

Disclosed are compositions and methods for detecting small quantities of analytes such as proteins and peptides. The method involves associating a primer with an analyte and subsequently using the primer to mediate rolling circle replication of a circular DNA molecule. Amplification of the DNA circle is dependent on the presence of the primer. Thus, the disclosed method produces an amplified signal, via rolling circle amplification, from any analyte of interest. The amplified DNA remains associated with the analyte, via the primer, and so allows spatial detection of the analyte. The disclosed method can be used to detect and analyze proteins and peptides. Multiple proteins can be analyzed using microarrays to which the various proteins are immobilized. A rolling circle replication primer is then associated with the various proteins using a conjugate of the primer and a molecule that specifically binds the proteins to be detectable. Rolling circle replication from the primers results in production of a large amount of DNA at the sites in the array where the proteins are immobilized. The amplified DNA serves as a readily detectable signal for the proteins. The disclosed method can also be used to compare the proteins expressed in two or more different samples. The information generated is analogous to the type of information gathered in nucleic acid expression profiles. The disclosed method allows sensitive and accurate detection and quantitation of proteins expressed in any cell or tissue.

The present invention is to provide calixcrown derivatives of formulae 1 to 3 requisite for the preparation of a self-assembled monolayer as well as a process for the preparation thereof. Further the present invention is to provide a self-assembled monolayer which is produced by immersing a gold substrate or related metal substrate in an organic solution containing said calixcrown derivatives of formulae 1 to 3. Still further the present invention provides a process for fixing a protein monolayer by fixing proteins having molecular weight of not less than 20,000D (20KD) on said self-assembled monolayer.

The invention discloses an enzyme-GO-MOFs nano composite catalyst and a preparation method thereof. The preparation method comprises the following steps: (1) adding a modifier into an enzyme liquid so as to obtain a modified enzyme solution, wherein the modifier is an N-vinyl amide polymer or pluronic F-127; the N-vinyl amide polymer is one or a mixture of more of polyvinylpyrrolidone, poly N-vinyl caprolactam and poly N-ethylene formamide; (2) mixing GO and a metallic salt solution, performing ultrasonic treatment, further adding an organic ligand solution into the mixed solution so as to obtain a GO-MOFs composite material synthesized stock solution, and performing ultrasonic treatment for another time; (3) adding a modified enzyme solution into the GO-MOFs composite material synthesized stock solution, and performing ultrasonic treatment so as to obtain a reaction liquid; (4) centrifuging the reaction liquid, collecting precipitate, washing the precipitate, and performing vacuum drying, thereby obtaining the enzyme-GO-MOFs nano composite catalyst. The preparation method disclosed by the invention is simple and feasible, and the nano composite material catalyst is gentle in synthesis condition and high in protein immobilization rate.

A high throughput screening method is described which employs a PVDF substrate for protein immobilization.

The present invention relates to a method for immobilizing a protein in a sample, which could not easily be immobilized by the conventional immobilization method, to a solid-phase; a method for quantitative determination of protein wherein an effect of inhibitory substance coexisting in a sample prepared using the immobilization method can be reduced; and a rapid and highly precise method for detecting an abnormal PrP and a method for determining BSE using the immobilization method as compared with the conventional method. The present invention provides: “a method for immobilizing a protein to a solid-phase comprising contacting the protein with the solid-phase having hydrophobic surface in the presence of a lower alcohol, and a halogenocarboxylic acid and/or a long chain alkyl sulfate, and an immobilizing reagent solution to be used therefor; a method for quantitative determination of protein comprising contacting a protein-staining solution with the solid-phase immobilized with a protein by the immobilization method, and determining a degree of color development generated thereby; an immunoblotting method wherein the solid-phase immobilized with a protein by the immobilization method is used; and a method for detecting an abnormal PrP a method for determining BSE by using the immobilization method.”

The invention discloses a living polymerization preparation method for a polyacrylic acid ball brush and application of the polyacrylic acid ball brush and particularly discloses the polyacrylic acid ball brush prepared by the living polymerization method and the application of the polyacrylic acid ball brush in the protein high density immobilization. The method comprises steps of synthesizing a novel silylanized reversible addition-fragmentation chain transfer (RAFT) polymeric chain transfer reagent; fixing the RAFT polymeric chain transfer reagent to a spherical solid phase carrier surface through a reaction; forming a polyacrylic acid brush structure on the carrier surface which is grafted with the RAFT polymeric chain transfer reagent through the RAFT polymerization; and obtaining the polyacrylic acid ball brush. The polyacrylic acid ball brush material is subjected to activation and protein immobilization, the compound of the ball brush and the protein is obtained, ant the application in the protein high density immobilization is achieved. By the aid of the method, the synthetic process and the synthetic time of the polyacrylic acid ball brush are shortened, the reaction efficiency is improved, and the polyacrylic acid ball brush has high grafting density and carboxyl content and provides more positions for protein immobilization.

The present invention provides a fibrous protein-immobilization system composition comprising a fiber comprising fiber-forming material, and a protein attached to the fiber-forming material.

It is intended to provide a novel method of immobilizing a protein and a protein chip, by which the protein can be immobilized at a high reproducibility while preventing the protein from inactivation without resort to a large-scaled apparatus and the protein can be immobilized even in a microchannel. Further, by using a cell adhesive protein as the protein to be immobilized, it is also possible to use a cell as a target and to provide a method of immobilizing a cell and a cell chip, by which a cell can be immobilized in an arbitrary region on a substrate.

To provide a measuring probe excellent in sensitivity and reproducibility, simple operation in the protein immobilization onto a support can prevent peeling or damage. A layer containing a protein is adsorbed onto a support surface to fully encircle the long axis of the support, and the adsorbed layer is treated for cross-linking with a cross-linking agent in a concentration of 1 to 5 moles to 1 mole of the protein.

The present invention relates to a method for preparing an protein monolayer using a peptide hybrid for protein immobilization, more precisely a peptide hybrid for protein immobilization which has improved solubility by introducing a PEG linker and a proper reaction group to the oligopeptide having specific affinity to selected types of proteins and is designed to provide enough space between solid substrates and proteins immobilized, whereby various solid substrates treated by the hybrid catch specific proteins effectively on. The peptide hybrid for protein immobilization of the present invention facilitates the control of orientation of an antibody on various solid surfaces and immobilization of various antibodies of different origins or having different isotypes with different affinity. Therefore, the surface treatment technique using the peptide hybrid of the invention can be effectively used for the production of various immunosensors and immune chips.

The invention relates to the field of covalently attaching proteins to a substrate, particularly to methods of immobilizing proteins by posttranslationally modifying a cysteine residue of said protein through the addition of functional groups. The invention also relates to biological molecules used in such techniques, including proteins, and detection methods and kits that utilize such immobilized proteins, such as a microdevice or “protein chip”, a high-throughput screening device, and for the microscopy of proteins on a surface.

An immobilized protein catalyst is prepared by applying an adhesive to a polymeric support, applying a layer of a globular protein over the layer of adhesive, binding a crosslinking agent to the protein layer, and binding the protein catalyst by reaction with the crosslinking agent.

Provided is a biomaterial immobilizing method including immobilizing a bovine serum albumin on a substrate, providing a biomaterial on the substrate immobilized with the bovine serum albumin, and irradiating an ultraviolet light onto the substrate provided with the bovine serum albumin and the biomaterial to immobilize the biomaterial selectively on the substrate immobilized with the bovine serum albumin.

It is intended to provide an anti-photocrosslinking group antibody capable of specifically binding to a photocrosslinking group and available in the binding of a substrate and a substance of interest in a microstructure, a complex protein including at least the antibody or at least a portion thereof, and a technique for use thereof in the detection of a target substance. The present invention provides a protein having at least the structure of an antibody that recognizes a photocrosslinking group.

A plastic substrate used for preparing biochip is a plastic carrier chip, to which the protein, peptide, or micromolecular compound is fixed after its surface is chemically treated, especially a polystyrene carrier chip, to which the oligonucleotide or polynucleotide is fixed after its surface is treated to obtain DNA chip.

The invention discloses a nonspecific adsorption prevention three-dimensional chip and application thereof. The chip comprises a solid supporting layer, an initiator layer, a click cross-linking layerand a polyethylene glycol methyl acrylate layer, wherein the initiator layer is bonded to the supporting layer through a Au-S bond; the click cross-linking layer is covalently bonded to the initiatorlayer; the polyethylene glycol methyl acrylate layer is bonded to the click cross-linking layer through a covalent bond. After protein is immobilized to the chip, the chip is used for detecting the interaction of the protein, and the chip can inhibit nonspecific adsorption of plasma and a cell lysis solution. The chip has the advantages of being novel in structural design, high in anti-protein adsorption capacity and capable of well inhibiting non-specific adsorption of the plasma and the cell lysis solution on the surface of the chip.

The invention relates to an immobilization method of cubilose water-soluble protein. The method comprises the following steps: soaking dry cubilose in ultrapure water; dehydrating the soaked cubilose, and steaming the dehydrated cubilose in a steaming box; cooling the steamed cubilose, and the cubilose water-soluble protein is immobilized after cooling. According to the immobilization method of the cubilose water-soluble protein, ultrapure water is adopted for soaking, the ultrapure water can be easily absorbed by the dry cubilose, the cubilose is easy to soak and is not easy to dissolve; a specific steaming temperature and a specific steaming time are adopted, so that the protein is comprehensively immobilized, and the protein can not be immobilized excessively. According to the method, the cooling temperature and time are controlled, rapid cooling is guaranteed, freshness is locked in time, when the temperature is reduced to 0-4 DEG C, the fresh-keeping temperature can be kept, and the storage time can be prolonged. By using the method provided by the invention, the cubilose water-soluble protein can be immobilized, the immobilization of protein is moderate, when the cubilose is cooked, the hardness is moderate, the eating and taste are not affected, and it is good for human absorption.

The invention provides a surface polylysine modifying method for a PDMS micro-fluidic chip.The PDMS micro-fluidic chip is subjected to plasma processing and silanization processing, and then coupled with polylysine, polylysine is firmly bound to the surface of the chip in a covalent binding mode, and the micro-fluidic chip capable of efficiently fixing protein is formed.According to the modified chip, the common advantages of being high in signal-to-noise ratio, high in binding capacity, low in inter-point variation coefficient and the like of a polylysine modifying method are kept, and more importantly, the problem that polylysine and the chip are bound infirmly in a physical absorption mode, and consequently polylysine is prone to fall off especially in microfluidics application is solved.