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Nonspecific adsorption prevention three-dimensional chip and application thereof

A three-dimensional chip, non-specific technology, applied in laboratory containers, measuring devices, material analysis through optical means, etc., can solve the problem of "pollution" on the surface of sensor chips, achieve good suppression effect, and have scientific and reasonable structural design , The effect of simple preparation steps

Active Publication Date: 2018-10-26
QIANNAN NORMAL UNIV FOR NATTIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology describes different ways for create small chips that have unique properties such as high sensitivity towards certain substances (protein) without being affected by other compounds like albumen). These tiny chips could be useful tools in biological researches where they help identify unknown molecules with very low concentrations.

Problems solved by technology

This patented describes an advanced method for detecting small molecules called chemical compounds or metabolites within body fluids quickly with minimal amounts needed compared to traditional analysis techniques like liquid chromatography. However, there may be issues related to how well these tiny particles can bind onto surfaces inside the device without causing contaminants during use.

Method used

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  • Nonspecific adsorption prevention three-dimensional chip and application thereof
  • Nonspecific adsorption prevention three-dimensional chip and application thereof
  • Nonspecific adsorption prevention three-dimensional chip and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] An anti-nonspecific adsorption three-dimensional chip, prepared by the following method:

[0054]1) Magnetron sputtering thickness is 1nm chrome film on clean glass substrate surface, subsequently, the gold film that thickness is about 30nm is magnetron sputtered on the surface of chrome film; Clean above-mentioned plated gold film with plasma cleaner Glass substrate, removes its pollutant that is adsorbed on the surface; The glass substrate coated with gold film after cleaning is soaked in the ethanol solution that contains the diluent (formula I) and initiator (formula I) that total concentration is 1mM immediately , wherein the molar ratio of the initiator to the diluent is 1:1000, the diluent and the initiator are self-assembled on the gold surface through the Au-S bond; after 5 hours of reaction, the glass substrate surface self-assembles with a single-layer initiator Floor.

[0055] 2) Under a protective gas atmosphere, add 1 mole part of alkynyl group-containing...

Embodiment 2

[0058] An anti-nonspecific adsorption three-dimensional chip, prepared by the following method:

[0059] 1) Magnetron sputtering thickness is 5nm chrome film on clean glass substrate surface, subsequently, the gold film that thickness is about 50nm is magnetron sputtered on the surface of chrome film; Clean above-mentioned plated gold film with plasma cleaner Glass substrate, remove its pollutant that is adsorbed on the surface; The glass substrate coated with gold film after cleaning is soaked in the ethanol solution that contains the diluent (formula II) and initiator (formula II) that total concentration is 1mM immediately , wherein the molar ratio of the initiator to the diluent is 100:1000, the diluent and the initiator are self-assembled on the gold surface through the Au-S bond; after 10 hours of reaction, the glass substrate surface is self-assembled with a monolayer structure Floor.

[0060] 2) Under a protective gas atmosphere, add 1 mole part of azide group-contain...

Embodiment 3

[0063] An anti-nonspecific adsorption three-dimensional chip, prepared by the following method:

[0064] 1) Magnetron sputtering thickness is 3nm chrome film on clean glass substrate surface, subsequently, the gold film that thickness is about 40nm is magnetron sputtered on the surface of chrome film; Clean above-mentioned plated gold film with plasma cleaner Glass substrate, removes its pollutant that is adsorbed on the surface; The glass substrate coated with gold film after cleaning is soaked in the ethanol solution that contains the diluent (formula III) and initiator (formula III) that total concentration is 1mM immediately , wherein the molar ratio of the initiator to the diluent is 20:1000, the diluent and the initiator are self-assembled on the gold surface through the Au-S bond; after 12 hours of reaction, the glass substrate surface is self-assembled with a single-layer initiator Floor.

[0065] 2) Under a protective gas atmosphere, add 1 mole part of alkynyl group-...

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Abstract

The invention discloses a nonspecific adsorption prevention three-dimensional chip and application thereof. The chip comprises a solid supporting layer, an initiator layer, a click cross-linking layerand a polyethylene glycol methyl acrylate layer, wherein the initiator layer is bonded to the supporting layer through a Au-S bond; the click cross-linking layer is covalently bonded to the initiatorlayer; the polyethylene glycol methyl acrylate layer is bonded to the click cross-linking layer through a covalent bond. After protein is immobilized to the chip, the chip is used for detecting the interaction of the protein, and the chip can inhibit nonspecific adsorption of plasma and a cell lysis solution. The chip has the advantages of being novel in structural design, high in anti-protein adsorption capacity and capable of well inhibiting non-specific adsorption of the plasma and the cell lysis solution on the surface of the chip.

Description

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Claims

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Application Information

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Owner QIANNAN NORMAL UNIV FOR NATTIES
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