92 results about "Protein adsorption" patented technology

The invention relates to the technical field of biological separation materials, and discloses an amine-functionalized magnetic nano composite material and application thereof in protein separation and purification. The material has a core-shell structure and comprises a magnetic ferroferric oxide nano core, an ordered mesoporous silicon dioxide intermediate layer and a covalent grafted polyethyleneimine functional layer. By controlling the matching relationship between the pore diameter of mesopores and the molecular size of the polymer and adopting a crosslinking strategy, the material has high magnetic responsiveness, rapid mass transfer performance and high-density surface amino groups. The material shows high adsorption capacity, rapid adsorption kinetics and good cycle stability in the protein separation process, and is suitable for enrichment purification and metal chelating affinity separation of protein in a biological sample. According to the invention, the technical problems of low protein adsorption capacity, slow dynamics and poor cycling stability caused by compact shell layer, non-uniform amino distribution and limited mass transfer of the existing amine functionalized magnetic material are solved.

The invention belongs to the technical field of biological pharmacy, and particularly relates to a preparation method and application of a nano-drug carrier. The invention provides a preparation process based on a pre-polymerization-coordination-shaping sequence, which comprises the following steps: firstly, slightly combining chitosan and polyphenol under a near-neutral condition to form small particles with uniform size and good dispersity, so that adhesion among the particles is effectively reduced; then ferric ions are slowly introduced under the regulation and control of a weak chelating agent, so that the ferric ions are uniformly coordinated on the surfaces of the particles, and an inner layer which is compact in structure and has responsiveness to an acid environment or a competitive ligand is constructed; and finally, sodium alginate is introduced, crosslinking is performed through slow-release calcium ions, a flexible outer layer is formed, and the layer and the inner layer have a synergistic effect, so that the dilution resistance and the protein adsorption resistance of the carrier are remarkably enhanced.

The invention relates to the technical field of blood purification materials, in particular to a zirconium-titanium bimetal MOF hollow microsphere adsorbent and a preparation method and application thereof. The adsorbent is based on a Zr / Ti bimetallic node and a phosphonic acid-carboxylic acid mixed ligand, a composite MOF hollow microsphere structure with high stability and high specific surface area is constructed through a solvothermal and polymerization synergistic reaction in a water-in-oil suspension system, a phosphonic acid group and Zr are strongly coordinated to improve the stability of a framework, and the composite MOF hollow microsphere structure has a high specific surface area. The carboxylic acid group keeps the hydrophilicity of the pore channel; meanwhile, a cross-linked network of acrylamide and N, N '-methylene bisacrylamide penetrates through crystal gaps in the balling process, so that the material has excellent mechanical strength and fluid stability; and a sulphobetaine amphoteric polymer brush layer is constructed on the surface of the MOF through endogenous initiation point modification and surface in-situ controllable free radical polymerization technologies, so that protein adsorption and platelet adhesion are effectively inhibited, the blood compatibility is remarkably improved, and meanwhile, the channel accessibility is maintained to promote rapid diffusion and adsorption of bilirubin.

The invention relates to the technical field of flexible bioelectronics and biomedical sensors, in particular to a biocompatible flexible carbon-based physiological electrode prepared through coaxial spinning and application of the biocompatible flexible carbon-based physiological electrode. The conductive core is formed by crosslinking graphene oxide, carbon nanotubes and the like; the buffer layer contains polyurethane and the like, and shape memory microspheres and antibacterial nano-silver are added; a biological interface layer contains chitosan and the like, and PEG-PE is grafted to reduce protein adsorption; the thickness ratio of the three layers is 1: (1.5-2): (0.5-1), and the thickness is 100-300 microns; the electrode has conductive, sensing and antibacterial functions, can load double enzymes for blood glucose monitoring, is strong in interlayer bonding, and is suitable for various biomedical scenes. By innovating the three-layer structure and the material design, high conductivity, high flexibility and excellent biocompatibility are achieved; multi-parameter monitoring and electrical stimulation treatment can be achieved, the anti-bending performance is good, the antibacterial and anticoagulant effects are good, the preparation process is controllable, and the application prospects in the fields of wearable equipment, implantation medical treatment and the like are wide.

The invention relates to the technical field of oral biological materials, in particular to a self-adaptive anti-caries coating as well as a preparation method and application thereof. The coating is prepared from random and block polymers, and is specifically prepared by polymerizing acid radical-containing functional monomers, phenylboronic acid and derivatives thereof. Construction of the coating depends on specific coordination of a functional group and Ca < 2 + > in enamel, and stable anchoring on the tooth surface is achieved. The core action mechanism is to introduce phenylboronic acid groups to endow the coating with pH sensitive characteristics: in a cariogenic acid environment, biofilm formation is synergistically inhibited through a plurality of mechanisms such as physical barrier, electrostatic repulsion and inhibition of mucoprotein-mediated adhesion. According to the application, the Ca < 2 + > anchoring monomer, phenylboronic acid and the derivative thereof are copolymerized, design is carried out aiming at two key links of protein adsorption and bacterial adhesion, multi-mechanism synergy and environment dynamic response are integrated, and an integrated anti-caries effect from early intervention to long-term protection is realized.

This invention discloses a magnetic nano-silica, its preparation method, and its application in the enrichment of low-abundance proteins. The proposed method for preparing magnetic silica, while preserving the surface properties of silica and maintaining its protein adsorption capacity, also imparts magnetism; providing a simpler, faster, and more efficient technique for large-scale sample processing. The low-abundance protein enrichment based on magnetic nano-silica proposed in this invention can be applied to almost all sample types, effectively solving the interference caused by high-abundance proteins on the identification of low-abundance proteins during mass spectrometry detection, and increasing the number of identified proteins by 100%-700%.

This invention discloses a magnetic nanomolecular sieve, its preparation method, and its application in the enrichment of low-abundance proteins. The preparation method of the magnetic nanomolecular sieve proposed in this invention is simple to operate, employing a one-pot method. It simultaneously imparts magnetism while maintaining the surface properties of the sieve, i.e., its protein adsorption capacity, thus preserving its magnetic properties. This provides a simpler, faster, and more efficient technical method for large-scale sample processing. The low-abundance protein enrichment based on magnetic molecular sieves proposed in this invention can be applied to almost all sample types, effectively solving the interference caused by high-abundance proteins on the identification of low-abundance proteins during mass spectrometry detection, and increasing the number of protein identifications by 100%-700%.

A method for purifying a protein comprising an antibody, antibody fragment, or immunoglobulin single variable domain, from a solution containing at least one contaminant by superantigen chromatography comprising: a) adsorbing the protein to the superantigen immobilized on a solid support; b) removing the at least one contaminant by contacting the immobilized superantigen containing the adsorbed protein with a first wash buffer comprising an aliphatic carboxylate; and c) eluting the protein from the superantigen immobilized on the solid support.

This invention relates to the field of medical device technology and provides a method for preparing a drug sustained-release coating and a medical device containing the same. The method includes forming a drug sustained-release coating on the surface of the medical device using a drug sustained-release polymer and a drug. The drug sustained-release polymer is a multi-component polymer containing functional groups for anti-protein adsorption, drug sustained-release, and chemical cross-linking. While ensuring the anticoagulant properties and adhesion to the surface of the medical device, the drug sustained-release coating, through the synergistic effect of butyl methacrylate and trimethoxysilyl propyl methacrylate, achieves an initial burst release of less than 20% of the total drug amount and slows down drug release to ensure a drug release cycle in vivo of no less than 21 days. This drug sustained-release coating can release a stable amount of drug to the tissue wall it contacts over a long period, aiming to achieve a long-term therapeutic or preventative effect on diseases such as luminal stenosis.

To provide a protein adsorption inhibitor that can suppress the adsorption of proteins to surfaces such as immunoreaction vessels and measuring instruments to a high degree, while also reducing variability between measurements. [Solution] Formula (1): Z-{O-[(PO) a (EO) b ]-(AO) c -H]} x A protein adsorption inhibitor comprising an alkylene oxide derivative represented by (the definition of the symbols in the formula is as described in the specification), wherein the cloud point of a 1% by mass aqueous solution of the alkylene oxide derivative is 0°C or higher and 30°C or lower.

The application belongs to the technical field of medical biomaterials, and provides a natural polysaccharide-polyphenol network polytetrafluoroethylene artificial blood vessel and a preparation method, wherein the natural polysaccharide-polyphenol network polytetrafluoroethylene artificial blood vessel comprises an expanded polytetrafluoroethylene artificial blood vessel base body, further comprises a polydopamine-acetone copolymerization intermediate layer attached to the whole surface of the base body, and a natural polysaccharide-polyphenol network coating layer covalently crosslinked to the inner surface of the intermediate layer; the natural polysaccharide-polyphenol network coating layer is formed by tannic acid as a natural crosslinking agent and sulfonated carboxymethyl chitosan through covalent bonds, hydrogen bonds and hydrophobic interaction; the outer surface of the base body is rich in methyl propionyl groups and retains hydrophobicity; the application has excellent anticoagulant properties, good biological safety, selective functionalization of the inner and outer surfaces, excellent anti-protein adsorption properties and good coating stability, and has a simple preparation process and low cost.

The invention belongs to the technical field of material surface performance detection, and particularly relates to a method for detecting biological pollution resistance and durability of a silicone rubber surface, and the method comprises the following steps: S1, sequentially carrying out biological membrane inhibition rate quantitative detection, antibacterial adhesion detection, protein adsorption resistance test and surface wettability test on a silicone rubber sample to be detected; s2, carrying out durability treatment on the parallel sample; s3, repeating partial or all tests in the step S1 on the sample treated in the step S2; s4, comparing the performance data obtained in the step S1 and the performance data obtained in the step S3, and evaluating the biological pollution resistance and durability of the silicone rubber sample. According to the invention, biological membrane inhibition rate quantitative detection, anti-bacterial adhesion detection, anti-protein adsorption performance test, surface wettability analysis and durability test are organically combined to form a complete evaluation closed loop from molecular adsorption to microbial community and from immediate efficiency to long-acting stability, and all key links of biological pollution are comprehensively covered.

This invention relates to a hyperbranched polylysine-modified macroporous chromatographic medium, its preparation method, and its application, belonging to the field of chromatographic separation technology. The preparation method includes the following steps: dispersing macroporous polysaccharide microspheres in a solvent to obtain a medium suspension; subsequently adding epichlorohydrin to the medium suspension and mixing thoroughly; adding sodium hydroxide solution to the medium suspension and stirring to obtain an epoxidized macroporous polysaccharide medium; immersing the epoxidized macroporous polysaccharide medium in a hyperbranched polylysine solution and stirring to ensure sufficient diffusion of the hyperbranched polylysine within the microspheres; the hyperbranched polylysine does not contain any hydrophobic groups; adding sodium hydroxide solution and sodium borohydride solution to the system and stirring to obtain the hyperbranched polylysine-modified macroporous chromatographic medium. Compared with existing technologies, this invention utilizes hyperbranched polylysine to modify macroporous chromatographic media, which can significantly improve protein adsorption capacity and adsorption rate.

To provide a method for adsorbing dropped protein to a base material having a titanium oxide layer on the surface at a high rate.SOLUTION: A method for adsorbing a protein to a substrate having a titanium oxide layer on a surface thereof, the method comprising: a protein solution dropping step of dropping a protein solution containing a protein onto the titanium oxide layer of the substrate; and a protein adsorption step of adsorbing the protein contained in the dropped protein solution onto the titanium oxide layer, wherein the protein solution contains 0.1 mg to 5 mg / mL of bovine serum albumin, and the substrate onto which the protein solution has been dropped is held for 85 minutes to 480 minutes in the protein adsorption step.SELECTED DRAWING: Figure 2

The present invention provides compositions and methods for reducing protein loss during drug delivery caused by protein adsorption onto one or more drug delivery system components. [Solution] In some embodiments, the present disclosure provides a composition for preventing the adsorption of proteins to one or more drug delivery system components, the composition comprising succinate and polysorbate 80. In some embodiments, the composition further comprises a therapeutic protein.

The application relates to the field of blood purification materials, and particularly discloses an anion exchange resin membrane applied to blood purification and a preparation method thereof. The anion exchange resin membrane applied to blood purification is prepared by blending a zwitterion-anion exchange copolymer and a hydrophobic film-forming polymer; the zwitterion-anion exchange copolymer is copolymerized by an anion exchange monomer and a zwitterion monomer, so that the resin membrane has anion exchange function and anti-protein adsorption function; the preparation method is as follows: the anion exchange monomer and the zwitterion monomer are subjected to free radical copolymerization under the action of an initiator; the copolymer and polyether sulfone are dissolved in a polar aprotic solvent to prepare a casting solution, and after casting film formation, phase inversion is completed by immersing in a coagulation bath; the obtained membrane is cleaned and subjected to alkalization treatment. The application solves the problems that traditional anion exchange resins easily cause non-specific protein adsorption, platelet adhesion and complement system activation.

Disclosed herein is a surfactant additive, ethylenediamine tetrakis(ethoxylate-block-propoxylate) tetrol with 16 ethylene oxide repeat units and 18 propylene oxide repeat units (known by its trade name as Tetronic 90R4) used as a droplet-additive, or to coat DMF driving electrode surfaces which dramatically improves the capability to work with high-protein-content liquids (e.g., whole blood) on digital microfluidic chips. This surfactant prevents protein adsorption and fouling of DMF electrode surfaces to an extent that was heretofore impossible. Specifically, this surfactant allows for the manipulation of droplets of undiluted whole blood for >1 hour per electrode (>1 50 times better than what is possible for any known additive). This improvement in handling high protein content media will revolutionize blood-based diagnostics on digital microfluidic platforms.

The present application relates to the use of polysialic acid or its derivatives in anti-foreign body reaction, in particular, to a polysialic acid anti-foreign body reaction material for resisting biological contamination, for regulating macrophage phenotype to reduce inflammatory response, and for resisting foreign body reaction. The polysialic acid material includes a bulk material prepared from polysialic acid and its derivatives, or a coating material for surface modification of the material, which can resist protein adsorption, cell adhesion and adhesion of various microorganisms, can act on immune cells, such as regulating the phenotype of macrophages, thereby reducing acute and chronic inflammatory response, and has excellent anti-foreign body reaction effect as an implant material.

The invention belongs to the technical field of biological materials, and particularly discloses a construction method of an anti-protein adsorption and antithrombotic graphene oxide coating, which comprises the following steps: step 1, carboxylating graphene oxide to obtain carboxylated graphene oxide nanoparticles; step 2, grafting heparin to the carboxylated graphene oxide nanoparticles; step 3, the graphene oxide-heparin nanoparticles are combined with polyethylene glycol, and graphene oxide-heparin-polyethylene glycol nanoparticles are obtained; and step 4, combining the graphene oxide-heparin-polyethylene glycol nanoparticles with a biological material. The preparation method has the advantages that the biocompatibility of the medical cardiovascular biological material is improved, the protein adsorption resistance and the thrombus resistance of the medical cardiovascular biological material are improved, and the in-vivo stability of the biological material is improved.

The invention provides a Pt / PEDOT composite modified carbon-based material needle-shaped electrode in-vivo dissolved oxygen electrochemical sensor as well as a construction method and application thereof, and belongs to the technical field of medical biology. According to the in-vivo dissolved oxygen electrochemical sensor, a carbon-based material microneedle type electrode is modified by electrochemical deposition of platinum nanoparticles by using a chloroplatinic acid hexahydrate solution and depending on a time current method, and poly (3, 4-ethylenedioxythiophene) is generated by direct electro-polymerization on the surface of the Pt-modified microneedle electrode through an electrochemical constant current method. And forming uniform PEDOT (poly (3, 4-ethylenedioxythiophene)) film coated and modified platinum nanoparticles on the microneedle electrode. The composite modified carbon-based material is used for replacing precious metal to reduce cost and improve biocompatibility, low-oxygen sensitivity is optimized by means of composite modification, in-vivo protein adsorption and biological membrane coverage are resisted through anti-pollution surface modification, and therefore the defects are overcome, and monitoring of in-vivo tissue oxygen partial pressure is achieved.

The invention relates to acrylic acid self-polishing antifouling resin capable of being stripped in a layered manner in seawater as well as a preparation method and application of the acrylic acid self-polishing antifouling resin. The preparation method of the antifouling resin comprises the steps of preparing a macromolecular acrylic acid organosilicon monomer, preparing a monomer mixture containing an initiator and the like. The acrylic acid self-polishing antifouling resin capable of being stripped in the seawater in a layered manner, which is prepared by the preparation method disclosed by the invention, shows good protein adsorption resistance and antibacterial growth resistance, and can be stripped in the seawater in a layered manner, so that stains adhered to the surface of a coating are released into a marine environment along with stripping of the coating; the surface of the new coating still can show an antifouling behavior, so that the new coating shows excellent antifouling capacity in actual ocean. The self-polishing antifouling resin is simple in preparation process and low in raw material cost, and has large-scale production and application prospects.

The invention discloses a modified ultrafiltration membrane, a preparation method thereof and a method for preparing a protein sample for mass spectrometry by adopting the modified ultrafiltration membrane, and belongs to the technical field of protein sample preparation. The surface of the ultrafiltration membrane is subjected to graft modification, so that the hydrophilicity of the surface of the membrane is effectively enhanced, that is, protein adsorption and membrane pollution are reduced by reducing the hydrophobicity of the surface of the membrane and increasing the hydrophilicity. According to the invention, amphoteric small molecules are connected to cellulose molecules of the ultrafiltration membrane, so that hydrophilic ends face outwards to mutually form hydrogen bonds to improve the hydrophilicity. Specifically, according to the method, the membrane is firstly subjected to alkaline activation, then amphoteric small molecules are inoculated, and the connection efficiency is improved. When the modified material is used for preparing a protein sample, the adsorption effect of the membrane on the protein sample can be reduced, the number of identified proteins and peptide fragments is finally increased, protein loss is reduced in the sample pretreatment step, and identification of more proteins and peptide fragments is facilitated.

The application discloses a molecular sieve membrane and a preparation method and application thereof in low-abundance protein enrichment, and the preparation method comprises the following steps: 1) polishing a support body to be smooth, drying after ultrasonic treatment; 2) adding an alcohol solution to the dried support body, then adding a certain amount of a coupling agent and ammonia water, taking out the support body after stirring, and drying the support body for standby use; 3) adding an alkali source into deionized water, completely dissolving, then adding a template agent, a stabilizer and a surfactant, and dissolving; 4) adding a silicon source and an alkali metal source into step 3), dissolving, then adding the dried support body in step 2), crystallizing at room temperature, then hydrothermally crystallizing, taking out the support body after the hydrothermal crystallization, washing, drying and calcining, so that the molecular sieve membrane is obtained. The molecular sieve membrane is simple in operation, the surface of the synthesized molecular sieve membrane is continuous and dense, the surface of the molecular sieve membrane retains the superior protein adsorption performance of the molecular sieve material, and the molecular sieve membrane can effectively improve the protein identification number.

A ketoreductase sequence is modified by adding a His tag, an immobilization carrier is a His-Tag protein adsorption magnetic bead, ketoreductase liquid is used as an active component, the modified ketoreductase is fixed on the protein adsorption magnetic bead carrier in a covalent binding manner, and the modified ketoreductase is immobilized on the protein adsorption magnetic bead carrier in a covalent binding manner. The obtained immobilized ketoreductase can be applied to preparation of ezetimibe intermediate ZT-5, and the method can greatly reduce the production cost, improve the traditional defects of difficulty in filtration, easiness in emulsification and the like, and can realize a real enzyme catalysis green and environment-friendly process.

This invention discloses a fluorinated membrane-fused liposome for modifying immune cells to regulate immunity, its preparation method, and its applications, belonging to the field of biomedical technology. This invention synthesizes a series of lipid molecules with different fluorinated chain lengths, including DOPE-F3, DOPE-F5, DOPE-F7, DOPE-F9, DOPE-F11, and DOPE-F13. Fluorinated membrane-fused liposomes are then prepared using these molecules in combination with other lipid components via a thin-film hydration method. These fluorinated membrane-fused liposomes exhibit excellent anti-protein adsorption capacity, efficient membrane fusion properties, and active spleen targeting. Experiments show that these fluorinated membrane-fused liposomes can achieve efficient membrane fusion with immune cells and effectively accumulate in the spleen in vivo, overcoming the shortcomings of traditional liposomes such as easy liver retention and low endocytosis efficiency. This invention has broad application prospects in the field of immunocellular therapy.

The application belongs to the technical field of biological pharmacy, and particularly relates to a preparation method of a nano drug carrier and application thereof. The application proposes a preparation process based on a pre-aggregation-coordination-shaping sequence: first, mild combination of chitosan and polyphenol is caused under near neutral conditions to form small particles with uniform size and good dispersibility, thereby effectively reducing the adhesion between particles; then, slowly introducing trivalent iron ions under the regulation of a weak chelating agent enables the trivalent iron ions to be uniformly coordinated on the particle surface, thereby constructing an inner layer with dense structure and responsiveness to acidic environment or competitive ligands; finally, introducing sodium alginate and cross-linking through slow release of calcium ions to form a flexible outer layer, and the outer layer and the inner layer synergistically act to significantly enhance the anti-dilution capacity and anti-protein adsorption performance of the carrier.

The invention relates to the field of bioengineering, in particular to a function-synergetic bionic gene editing carrier system as well as a preparation method and application thereof. The invention provides a gene editing expression box. The gene editing expression box comprises a cancer cell specific promoter, a CXCL9 gene, an sgBAG3 gene and an sgHSP70 gene. A bionic lipid carrier with protein adsorption resistance, cancer cell targeting and membrane fusion delivery is constructed, and an integrated gene editing CRISPR system capable of highly expressing CXCL9 protein factors in cancer cells and double editing tumor cell BAG3 and HSP70 genes is entrapped, so that a multifunctional synergetic bionic gene editing carrier system is constructed; according to the invention, in-vivo delivery stability of the CRISPR system, cancer cell targeting accuracy and intracellular release high efficiency are realized, liver cancer cell gene editing efficiency is improved, a T cell killing enhancement effect tumor immune microenvironment is constructed, and effective cancer resistance of the CRISPR system in a tumor environment is realized.

The invention discloses a controllable catheter which comprises an in-vivo implantation unit, an in-vitro control unit and an external auxiliary assembly. The in-vivo implantation unit is a medical silicon rubber hollow tube body and has seven specifications of 3.5-6.5 cm, a petal-shaped fixing part close to the bladder end can be unfolded and anchored on the bladder wall, and a magnetic drive pump valve assembly is arranged in the middle of the in-vivo implantation unit; the in-vitro control unit drives the spiral impeller to conduct negative pressure drainage through magnetic coupling, and the key controls opening and closing of the one-way valve. The size adaptation assembly comprises a urethra measurer and a push rod. The petal-shaped fixing part is coated with an antibacterial coating, the magnetic coupling efficiency between the inner magnet and the outer magnet is larger than or equal to 85%, and magnetic field leakage is controllable. The in-vitro unit integrates pressure monitoring and alarming functions and supports wireless charging and disinfection. And protein adsorption is reduced through in-vivo unit surface treatment, and the sealing of the joint reaches the IP67 standard. The device realizes autonomous urination control through magnetic driving, precise adaptation and antibacterial design, reduces the infection risk, improves the autonomy of patients, and is suitable for patients with urination dysfunction.

The application provides a blood perfusion adsorbent with low protein adsorption, a preparation method thereof and a blood perfusion device, and belongs to the technical field of blood purification adsorbents. The application introduces vinyl silicone oil into the adsorbent, the vinyl silicone oil has low surface energy, excellent anti-protein adsorption performance and tissue and blood compatibility, and the surface of the cross-linked silicone oil modified adsorption resin prepared through suspension polymerization has a pore structure, so that the adsorbent has good blood compatibility, medium and large molecule toxin adsorption performance and anti-protein adsorption performance. The cross-linked silicone oil modified adsorption resin is further subjected to cross-linking reaction to obtain an ultra-high cross-linked silicone oil modified adsorption resin, and the ultra-high cross-linked silicone oil modified adsorption resin still has anti-protein adsorption performance.

The utility model provides a urine protein adsorption device. The urine protein adsorption device comprises a filter column, an adsorption part, a guide column and an upper cover, the adsorption piece is arranged in the filter column; the guide column penetrates through the adsorption part in the axial direction, the lower end of the guide column is connected with a lower filter disc, the lower filter disc abuts against the lower end of the adsorption part, the upper end of the guide column is connected with a handle, and the handle can be contained in the guide column; the upper cover is screwed at the top of the filter column, the upper cover is connected with a sleeve, the lower end of the sleeve is connected with an upper filter disc, the upper filter disc is pressed at the upper end of the adsorption part, and the sleeve is sleeved outside the guide column. After the upper cover is dismounted, the handle is moved out of the guide column, the guide column is pulled out through the handle, the adsorption part can be synchronously brought out, and the adsorption part can be conveniently taken out.