163 results about "Protein adsorption" patented technology

The invention belongs to the technical field of hydrogel materials, and discloses a carboxylated chitosan modified silicone hydrogel contact lens material and a preparation method thereof. The preparation method comprises the following steps: mixing a siloxane monomer, a zwitterionic monomer, a first cross-linking agent, an initiator and a first organic solvent, and carrying out polymerization reaction to obtain silicone hydrogel; and mixing the silicone hydrogel, carboxylated chitosan, a second cross-linking agent and a second organic solvent, and carrying out a cross-linking reaction to obtain the carboxylated chitosan modified silicone hydrogel contact lens material. The zwitterionic monomer is introduced into the siloxane monomer polymerization process, and the carboxylated chitosan is adopted to modify the silicone hydrogel, so that the protein adsorption resistance and antibacterial property of the contact lens material are effectively improved. Meanwhile, the raw materials are wide in source, the preparation method is simple and easy to operate, and large-scale industrial production can be achieved easily.

The invention constructs a universal visual virus molecularly imprinted sensor modified with an anti-protein coating, the sensor uses magnetic Fe3O4 as an imprinting carrier, HBV as a template and dopamine as a functional monomer for imprinting to obtain a molecularly imprinted polymer with a polydopamine coating (PDA), and before the template is removed, the molecular imprinting polymer with the polydopamine coating (PDA) is separated from the template, so that the molecular imprinting polymer with the polydopamine coating (PDA) is obtained. The surface of the PDA layer is covered with a slightly cross-linked PMEO2MA layer through a water precipitation polymerization method, and the polymer can effectively reduce non-specific protein adsorption; and finally, exposing an imprinting cavity of the PDA layer by eluting the template molecules. On the other hand, potassium permanganate (KMnO4) is used as a signal output molecule, and when no target object exists, the strong reducibility of PDA can enable KMnO4 entering the imprinting cavity to be subjected to a color fading reaction. Based on the principle, high-specificity colorimetric visual detection on the HBV is realized. The sensor can be combined with a smart phone through naked eyes to carry out visual semi-quantitative analysis and carry out quantitative detection through ultraviolet rays, and the universality of the sensor can be confirmed through a template replacement experiment. And meanwhile, the sensor shows an excellent cost advantage. The diagnosis which is high in sensitivity, low in cost and capable of achieving rapid visualization shows huge potential in practical application, and particularly has important application value in infectious disease screening and monitoring work in areas with limited resources.

The invention relates to the field of hydrogel preparation, and discloses anti-scale zwitter-ion hydrogel as well as a preparation method and application thereof. The method comprises the following steps: firstly, initiating an oxazine derivative monomer to polymerize through a double-end initiator to generate dihydroxyl polyoxazine; and then, removing a side chain of the polymer to obtain the dihydroxy polypropylene imine. Then, dihydroxy polypropylene imine is selected for further reaction to generate a zwitter-ion precursor polymer, and the zwitter-ion precursor polymer is hydrolyzed to obtain the polypropylene imine zwitter-ion derivative. And finally, converting hydroxyl groups at two ends, synthesizing a zwitterionic macromolecular cross-linking agent, and carrying out polymerization cross-linking in an aqueous solution to prepare the anti-scale zwitterionic hydrogel. The hydrogel is simple in formula, is completely composed of the zwitterionic cross-linking agent, has excellent anti-scaling performance such as protein adsorption resistance, bacterial adsorption resistance and cell adsorption resistance, and is suitable for biological materials, medical devices, implant materials and cell delivery materials.

The invention relates to the technical field of biological separation materials, and discloses an amine-functionalized magnetic nano composite material and application thereof in protein separation and purification. The material has a core-shell structure and comprises a magnetic ferroferric oxide nano core, an ordered mesoporous silicon dioxide intermediate layer and a covalent grafted polyethyleneimine functional layer. By controlling the matching relationship between the pore diameter of mesopores and the molecular size of the polymer and adopting a crosslinking strategy, the material has high magnetic responsiveness, rapid mass transfer performance and high-density surface amino groups. The material shows high adsorption capacity, rapid adsorption kinetics and good cycle stability in the protein separation process, and is suitable for enrichment purification and metal chelating affinity separation of protein in a biological sample. According to the invention, the technical problems of low protein adsorption capacity, slow dynamics and poor cycling stability caused by compact shell layer, non-uniform amino distribution and limited mass transfer of the existing amine functionalized magnetic material are solved.

The present invention discloses a magnetic nanocomposite material, a preparation method thereof, and an application thereof in the enrichment of low-abundance proteins. The magnetic nanocomposite material proposed by the present invention is Fe3O4@TS-1. Its preparation method is simple to operate. The sol-gel hydrothermal method is adopted, and the preparation is stable. The magnetic composite material Fe3O4@TS-1 is successfully synthesized. While ensuring the material properties of the TS-1 molecular sieve material, this material not only guarantees excellent protein adsorption performance but also endows it with magnetism, providing a simpler, faster, and more efficient technical method for large-scale sample processing. The enrichment of low-abundance proteins based on the magnetic nanocomposite material proposed by the present invention can be applied to almost all sample types, effectively solving the interference caused by high-abundance proteins to the identification of low-abundance proteins during mass spectrometry detection, and the number of protein identifications can be increased by 100% - 700%.

The invention belongs to the technical field of biological pharmacy, and particularly relates to a preparation method and application of a nano-drug carrier. The invention provides a preparation process based on a pre-polymerization-coordination-shaping sequence, which comprises the following steps: firstly, slightly combining chitosan and polyphenol under a near-neutral condition to form small particles with uniform size and good dispersity, so that adhesion among the particles is effectively reduced; then ferric ions are slowly introduced under the regulation and control of a weak chelating agent, so that the ferric ions are uniformly coordinated on the surfaces of the particles, and an inner layer which is compact in structure and has responsiveness to an acid environment or a competitive ligand is constructed; and finally, sodium alginate is introduced, crosslinking is performed through slow-release calcium ions, a flexible outer layer is formed, and the layer and the inner layer have a synergistic effect, so that the dilution resistance and the protein adsorption resistance of the carrier are remarkably enhanced.

The invention relates to the technical field of medical materials, in particular to a self-lubricating PEBAX / TPU material suitable for a medical catheter and a preparation process of the self-lubricating PEBAX / TPU material. 8-15 parts of a self-lubricating modified material; 1.5 to 3 parts of an antioxidant complexing agent; and 3-5 parts of a compatilizer. According to the self-lubricating PEBAX / TPU material, fluorocarbon chains are enriched on the surface to form a long-acting low-friction layer through fluorinated polyethylene and hydrophobic nano silicon dioxide, an external coating is not needed, an antioxidant Irganox 1010 and carbodiimide synergistically inhibit oxidation and hydrolysis, the chemical stability is remarkably improved, the compatibility of PEBAX / TPU is improved through maleic anhydride grafted POE, and the self-lubricating PEBAX / TPU material has the advantages that the self-lubricating performance is good; the rigid gradient of the conduit is optimized by combining a multi-layer co-extrusion process, the protein adsorption resistance of the surface is further enhanced through plasma treatment and perfluorosilane grafting, the integrity of the material is guaranteed through accurately controlled low-temperature extrusion and low-dose gamma sterilization, and the material is more stable and reliable while the biological safety is maintained. The synergistic improvement of the lubrication durability, the mechanical property and the aging resistance is realized.

The utility model provides an exosome separation and purification device and relates to the technical field of medical instruments. Comprising a chromatographic column and a negative pressure concentration plate, the chromatographic column is internally provided with filler with an adsorption function, and the upper end of the filler is provided with a space serving as a sample adding area; the chromatographic column is provided with a filler position, and at least part of the chromatographic column extends into the ultrafiltration box of the negative pressure concentration plate; wherein the filler is a chromatographic filler with a protein adsorption function or an exosome adsorption function; the negative pressure concentration plate is also provided with a bottom box, the side surface of the ultrafiltration box is hermetically connected to the upper end of the bottom box, and a box groove is formed in the bottom box; and the side surface of the bottom box is provided with an extraction opening above the box groove. The exosome separation and purification device combines the advantages of a chromatographic column and a negative pressure concentration plate, has the characteristics of multifunctionality, simplicity and convenience in operation, less sample loss, good sealing performance and the like, and is suitable for separation and purification processes of exosomes.

Provided is a technique for improving the efficiency of adsorption of a protein onto a carrier in an apparatus equipped with an in-line mixer and a hydrocyclone. An adsorption method disclosed herein is performed using an apparatus equipped with an in-line mixer and a hydrocyclone connected to the in-line mixer. The adsorption method is a method in which a protein is adsorbed onto a carrier provided with a ligand capable of binding to the protein. The adsorption method includes: mixing a first slurry containing a carrier with a second slurry containing a protein using an in-line mixer to prepare a mixed slurry; and adsorbing the protein onto the carrier in the mixed slurry to produce a complex between the protein and the carrier. The mixed slurry is prepared so as to contain the carrier in an amount of 10-25 vol% and the protein in an amount of 5-20 mg per 1 ml of the carrier when the whole amount of the mixed slurry is 100 vol%. The mixing time in the inline mixer is 0.6-60 seconds.

A method for purifying a protein comprising an antibody, antibody fragment, or immunoglobulin single variable domain, from a solution containing at least one contaminant by superantigen chromatography comprising: a) adsorbing the protein to the superantigen immobilized on a solid support; b) removing the at least one contaminant by contacting the immobilized superantigen containing the adsorbed protein with a first wash buffer comprising an aliphatic carboxylate; and c) eluting the protein from the superantigen immobilized on the solid support.

The invention provides porous fiber aerogel as well as a preparation method and application thereof in protein adsorption separation. The porous fiber aerogel comprises alginic acid fibers and sodium alginate, the alginic acid fibers and the sodium alginate are mixed and then subjected to directional freezing forming through liquid nitrogen, and a regular vertical hole structure is formed through calcium ion crosslinking. The provided porous fiber aerogel has a high specific surface area, the surface of the porous fiber aerogel is provided with a large number of carboxyl functional groups, the porous fiber aerogel can be combined with a large number of positively charged proteins through electrostatic interaction and functional group effects to adsorb and separate the proteins, and the porous fiber aerogel has a good adsorption effect.

The invention discloses a hemodialysis membrane with a self-anticoagulation function and a preparation method of the hemodialysis membrane. The hemodialysis membrane with the self-anticoagulation function is obtained by modifying natural polymer fibrin, polydopamine and a sulfonic acid type polymer solution. The hemodialysis membrane is any one or more of a polysulfone membrane, a polyether sulfone membrane, a polyvinylidene fluoride membrane, a polymethyl methacrylate membrane, a polyacrylonitrile membrane, a polyamide membrane, a polyvinyl alcohol membrane, a cellulose acetate membrane and a polypropylene membrane. The invention is used for treating acute and chronic kidney diseases, has good biocompatibility, and shows good blood compatibility in the aspects of inhibiting protein adsorption, platelet adsorption and red blood cell adsorption, reducing hemolysis rate, prolonging blood coagulation time and the like. And the risk of thrombosis caused by improper use of an anticoagulant in a continuous anticoagulation treatment process can be effectively avoided, and the living quality and the survival time of a hemodialysis patient are effectively improved and prolonged.

The present invention discloses a polyethersulfone membrane with low protein adsorption function and its preparation method. The polyethersulfone membrane comprises the following raw materials according to mass percentage: 10-30% of polyethersulfone, 3-15% of amphiphilic copolymer, 2-10% of pore former, 0.5-15% of non-solvent, and 55-75% of solvent. Through a carefully designed formulation and preparation process, the present invention successfully prepares a polyethersulfone membrane with low protein adsorption function. The addition of the amphiphilic copolymer P(PVP-r-PDMS) not only enhances the hydrophilicity and protein adsorption resistance of the membrane, but also realizes the optimization of the membrane structure through the fine regulation of the RTIPS method. Finally, a polyethersulfone hollow fiber membrane with excellent anti-fouling performance and high permeability is obtained, which has broad application prospects in the fields of hemodialysis, drug filtration, food industry, etc.

The invention relates to the technical field of blood purification materials, in particular to a zirconium-titanium bimetal MOF hollow microsphere adsorbent and a preparation method and application thereof. The adsorbent is based on a Zr / Ti bimetallic node and a phosphonic acid-carboxylic acid mixed ligand, a composite MOF hollow microsphere structure with high stability and high specific surface area is constructed through a solvothermal and polymerization synergistic reaction in a water-in-oil suspension system, a phosphonic acid group and Zr are strongly coordinated to improve the stability of a framework, and the composite MOF hollow microsphere structure has a high specific surface area. The carboxylic acid group keeps the hydrophilicity of the pore channel; meanwhile, a cross-linked network of acrylamide and N, N '-methylene bisacrylamide penetrates through crystal gaps in the balling process, so that the material has excellent mechanical strength and fluid stability; and a sulphobetaine amphoteric polymer brush layer is constructed on the surface of the MOF through endogenous initiation point modification and surface in-situ controllable free radical polymerization technologies, so that protein adsorption and platelet adhesion are effectively inhibited, the blood compatibility is remarkably improved, and meanwhile, the channel accessibility is maintained to promote rapid diffusion and adsorption of bilirubin.

The invention relates to an anticoagulant medical device and a preparation method thereof. The preparation method of the anticoagulant medical device comprises the following steps: covalently connecting the hydrophilic anticoagulant layer on the surface of the base material in an atom transfer radical polymerization manner; a heparin anticoagulation layer is covalently connected to the surface of a hydrophilic anticoagulation layer to prepare the anticoagulation medical device, and the heparin anticoagulation layer and the hydrophilic anticoagulation layer are connected through one or more covalent bonds of a triazole bond, an amido bond, a carbon-nitrogen single bond and a carbon-sulfur single bond. According to the preparation method of the anticoagulation medical device, the anti-protein adsorption effect of the hydrophilic anticoagulation layer and the anticoagulation effect of the heparin anticoagulation layer can be synergistically exerted, the anticoagulation effect is improved, and meanwhile, the anticoagulation stability is improved.

The invention relates to the technical field of flexible bioelectronics and biomedical sensors, in particular to a biocompatible flexible carbon-based physiological electrode prepared through coaxial spinning and application of the biocompatible flexible carbon-based physiological electrode. The conductive core is formed by crosslinking graphene oxide, carbon nanotubes and the like; the buffer layer contains polyurethane and the like, and shape memory microspheres and antibacterial nano-silver are added; a biological interface layer contains chitosan and the like, and PEG-PE is grafted to reduce protein adsorption; the thickness ratio of the three layers is 1: (1.5-2): (0.5-1), and the thickness is 100-300 microns; the electrode has conductive, sensing and antibacterial functions, can load double enzymes for blood glucose monitoring, is strong in interlayer bonding, and is suitable for various biomedical scenes. By innovating the three-layer structure and the material design, high conductivity, high flexibility and excellent biocompatibility are achieved; multi-parameter monitoring and electrical stimulation treatment can be achieved, the anti-bending performance is good, the antibacterial and anticoagulant effects are good, the preparation process is controllable, and the application prospects in the fields of wearable equipment, implantation medical treatment and the like are wide.

The invention relates to a PVDF (Polyvinylidene Fluoride) polymer lateral chromatography membrane and a preparation method thereof, and according to the method, a large-aperture PVDF membrane is prepared by blending a hydrophilic additive with high molecular weight and PVDF powder. The method comprises the following steps: mixing PVDF powder with a hydrophilic pore-foaming agent (such as PVP, PEG and the like) by adopting a solution blending method, performing non-solvent induced phase separation (NIPS) to form a membrane, immersing the membrane into a coagulating bath for a period of time, transferring the membrane into deionized water for soaking, taking out the membrane after the solvent is completely removed, and drying the membrane at normal temperature to form the membrane. And immersing the membrane in a surfactant solution for a period of time, taking out the membrane, and naturally airing the membrane so as to obtain the PVDF flat membrane which has a macroporous structure, a good pore structure, hydrophilicity and protein adsorption performance.

The invention discloses a non-degradable anti-protein adsorption material as well as a preparation method and application thereof in the field of anti-protein adsorption. The non-degradable anti-protein adsorption material is a block polymer taking a silane coupling agent as a crosslinking site or a material formed by crosslinking materials including the block polymer; the block polymer has non-degradability, a molecular chain comprises polyethylene glycol hydrophilic chain segments, hydrophobic chain segments introduced through hydrophobic monomers and cross-linking chain segments introduced through cross-linking monomers, and the polyethylene glycol hydrophilic chain segments are located between the hydrophobic chain segments. The hydrophobic chain segment is one or a combination of more of a methacrylate chain segment and an aryl-substituted polyolefin chain segment, and the crosslinking monomer is a silane coupling agent with a carbon-carbon double bond. The preparation method comprises the following steps: carrying out a controllable / active free radical polymerization reaction to obtain a block polymer; or after the block polymer is obtained according to the process, the non-degradable anti-protein adsorption material is further obtained through thermal crosslinking.

The invention discloses a preparation method of a drug-coated stent. The preparation method comprises the following steps: preparing a NiTi alloy shape memory stent; preparing a PMEA-based drug-loaded coating suspension slurry; and coating the prepared PMEA-based drug-loaded coating suspension slurry on the outer surface of the NiTi alloy shape memory stent by adopting a liquid flame spraying mode to obtain the drug-coated stent. The invention also discloses the drug-coated stent prepared by the preparation method and application thereof. The liquid flame spraying technology is used for stent surface modification, the prepared drug-coated stent has a good effect of preventing protein adsorption, bile duct re-blockage and stent failure can be better prevented by combining the drug-coated stent with antibacterial drugs, and the drug-coated stent can be used for clinical treatment of liver and gall diseases.

The invention discloses a core-shell type dextran-agarose compound polysaccharide gel microsphere as well as a preparation method and an application of the core-shell type dextran-agarose compound polysaccharide gel microsphere. The preparation method comprises the following steps: dissolving a shell layer material into deionized water to form a solution, and uniformly mixing the solution with core agarose microspheres to serve as a water phase; adding an emulsifier into a hydrophobic organic reagent to form a solution as an oil phase; mixing the water phase and the oil phase, emulsifying, adding 1-10w / w% of a cross-linking agent, and curing under an alkaline condition; demulsification and centrifugal separation are carried out, washing is carried out for many times, and the finished core-shell structured agarose microspheres are obtained. Domestic agarose is used as a raw material, so that the quality of the agarose microspheres is improved while the production cost is reduced; the shell layer of the core-shell structure microsphere provides more modification sites, so that the protein adsorption capacity is increased, and the mechanical strength of the microsphere is also greatly improved, thereby improving the separation efficiency of the microsphere.

The invention relates to the technical field of oral biological materials, in particular to a self-adaptive anti-caries coating as well as a preparation method and application thereof. The coating is prepared from random and block polymers, and is specifically prepared by polymerizing acid radical-containing functional monomers, phenylboronic acid and derivatives thereof. Construction of the coating depends on specific coordination of a functional group and Ca < 2 + > in enamel, and stable anchoring on the tooth surface is achieved. The core action mechanism is to introduce phenylboronic acid groups to endow the coating with pH sensitive characteristics: in a cariogenic acid environment, biofilm formation is synergistically inhibited through a plurality of mechanisms such as physical barrier, electrostatic repulsion and inhibition of mucoprotein-mediated adhesion. According to the application, the Ca < 2 + > anchoring monomer, phenylboronic acid and the derivative thereof are copolymerized, design is carried out aiming at two key links of protein adsorption and bacterial adhesion, multi-mechanism synergy and environment dynamic response are integrated, and an integrated anti-caries effect from early intervention to long-term protection is realized.

This invention discloses a magnetic nano-silica, its preparation method, and its application in the enrichment of low-abundance proteins. The proposed method for preparing magnetic silica, while preserving the surface properties of silica and maintaining its protein adsorption capacity, also imparts magnetism; providing a simpler, faster, and more efficient technique for large-scale sample processing. The low-abundance protein enrichment based on magnetic nano-silica proposed in this invention can be applied to almost all sample types, effectively solving the interference caused by high-abundance proteins on the identification of low-abundance proteins during mass spectrometry detection, and increasing the number of identified proteins by 100%-700%.

This invention discloses a magnetic nanomolecular sieve, its preparation method, and its application in the enrichment of low-abundance proteins. The preparation method of the magnetic nanomolecular sieve proposed in this invention is simple to operate, employing a one-pot method. It simultaneously imparts magnetism while maintaining the surface properties of the sieve, i.e., its protein adsorption capacity, thus preserving its magnetic properties. This provides a simpler, faster, and more efficient technical method for large-scale sample processing. The low-abundance protein enrichment based on magnetic molecular sieves proposed in this invention can be applied to almost all sample types, effectively solving the interference caused by high-abundance proteins on the identification of low-abundance proteins during mass spectrometry detection, and increasing the number of protein identifications by 100%-700%.

To provide an ET adsorbent whose base material is an inorganic material and which is capable of effectively adsorbing ET while inhibiting adsorption of protein.SOLUTION: An endotoxin adsorbent includes an inorganic base material having a cationic group including nitrogen atom, and has no pore or has a pore whose diameter is 40 nm or less. A material of the inorganic base material is preferably a silica base material. The cationic group including nitrogen atom may be bonded to the inorganic base material with or without a spacer interposed therebetween.SELECTED DRAWING: None

A method for purifying a protein comprising an antibody, antibody fragment, or immunoglobulin single variable domain, from a solution containing at least one contaminant by superantigen chromatography comprising: a) adsorbing the protein to the superantigen immobilized on a solid support; b) removing the at least one contaminant by contacting the immobilized superantigen containing the adsorbed protein with a first wash buffer comprising an aliphatic carboxylate; and c) eluting the protein from the superantigen immobilized on the solid support.

The invention belongs to the technical field of artificial blood vessels, and provides a composite modified double-layer structure small-caliber artificial blood vessel which comprises an inner layer and an outer layer, the inner layer comprises a mixture of zwitterionic polyurethane and polycaprolactone, the outer layer comprises polycaprolactone, zwitterionic polyurethane contains zwitterionic sulfonic acid negative ions and ammonium positive ions, and the zwitterionic sulfonic acid negative ions and the ammonium positive ions are different. Inner and outer layers of the artificial blood vessel are modified by heparin; the invention further provides a preparation method of the artificial blood vessel, the double-layer artificial blood vessel with a zwitterionic polyurethane and polycaprolactone blend as an inner layer and polycaprolactone as an outer layer is prepared through electrostatic spinning, and heparin is used for surface modification of the double-layer artificial blood vessel. The size of the small-caliber artificial blood vessel can be effectively controlled, and the prepared small-caliber antithrombotic artificial blood vessel has excellent biocompatibility, tensile property, protein adsorption resistance and antithrombotic property and can be used for vascular tissue repair or replacement.

The invention discloses an extraction method of tremella sporocarp extracellular polysaccharide and application of the tremella sporocarp extracellular polysaccharide in skin repair, and relates to a Ni < 2 + > modified tannic acid modified mesoporous material and a method for preparing high-purity tremella sporocarp extracellular polysaccharide through physical adsorption of protein, the method comprises the following steps: covering the surface of a mesoporous SBA-15 material with tannic acid, and then adding Ni < 2 + > ions for unsaturated chelating; the Ni < 2 + > modified tannic acid modified mesoporous protein adsorption material is prepared; the method comprises the following steps: fully soaking tremella, fully dissolving tremella sporocarp extracellular polysaccharide in water, concentrating and centrifugally separating the sporocarp extracellular polysaccharide solution to remove particle impurities, and removing protein through a mesoporous protein adsorption material. The method adopts pure physical extraction of tremella sporocarp exopolysaccharide, and has the characteristics of simple process, capability of improving resource utilization, suitability for factory continuous production, no organic solvent residue, high product purity, good skin health effect and the like.

This invention relates to the field of medical device technology and provides a method for preparing a drug sustained-release coating and a medical device containing the same. The method includes forming a drug sustained-release coating on the surface of the medical device using a drug sustained-release polymer and a drug. The drug sustained-release polymer is a multi-component polymer containing functional groups for anti-protein adsorption, drug sustained-release, and chemical cross-linking. While ensuring the anticoagulant properties and adhesion to the surface of the medical device, the drug sustained-release coating, through the synergistic effect of butyl methacrylate and trimethoxysilyl propyl methacrylate, achieves an initial burst release of less than 20% of the total drug amount and slows down drug release to ensure a drug release cycle in vivo of no less than 21 days. This drug sustained-release coating can release a stable amount of drug to the tissue wall it contacts over a long period, aiming to achieve a long-term therapeutic or preventative effect on diseases such as luminal stenosis.

To provide a protein adsorption inhibitor that can suppress the adsorption of proteins to surfaces such as immunoreaction vessels and measuring instruments to a high degree, while also reducing variability between measurements. [Solution] Formula (1): Z-{O-[(PO) a (EO) b ]-(AO) c -H]} x A protein adsorption inhibitor comprising an alkylene oxide derivative represented by (the definition of the symbols in the formula is as described in the specification), wherein the cloud point of a 1% by mass aqueous solution of the alkylene oxide derivative is 0°C or higher and 30°C or lower.

The application belongs to the technical field of medical biomaterials, and provides a natural polysaccharide-polyphenol network polytetrafluoroethylene artificial blood vessel and a preparation method, wherein the natural polysaccharide-polyphenol network polytetrafluoroethylene artificial blood vessel comprises an expanded polytetrafluoroethylene artificial blood vessel base body, further comprises a polydopamine-acetone copolymerization intermediate layer attached to the whole surface of the base body, and a natural polysaccharide-polyphenol network coating layer covalently crosslinked to the inner surface of the intermediate layer; the natural polysaccharide-polyphenol network coating layer is formed by tannic acid as a natural crosslinking agent and sulfonated carboxymethyl chitosan through covalent bonds, hydrogen bonds and hydrophobic interaction; the outer surface of the base body is rich in methyl propionyl groups and retains hydrophobicity; the application has excellent anticoagulant properties, good biological safety, selective functionalization of the inner and outer surfaces, excellent anti-protein adsorption properties and good coating stability, and has a simple preparation process and low cost.