125 results about "Protein adsorption" patented technology

The invention constructs a universal visual virus molecularly imprinted sensor modified with an anti-protein coating, the sensor uses magnetic Fe3O4 as an imprinting carrier, HBV as a template and dopamine as a functional monomer for imprinting to obtain a molecularly imprinted polymer with a polydopamine coating (PDA), and before the template is removed, the molecular imprinting polymer with the polydopamine coating (PDA) is separated from the template, so that the molecular imprinting polymer with the polydopamine coating (PDA) is obtained. The surface of the PDA layer is covered with a slightly cross-linked PMEO2MA layer through a water precipitation polymerization method, and the polymer can effectively reduce non-specific protein adsorption; and finally, exposing an imprinting cavity of the PDA layer by eluting the template molecules. On the other hand, potassium permanganate (KMnO4) is used as a signal output molecule, and when no target object exists, the strong reducibility of PDA can enable KMnO4 entering the imprinting cavity to be subjected to a color fading reaction. Based on the principle, high-specificity colorimetric visual detection on the HBV is realized. The sensor can be combined with a smart phone through naked eyes to carry out visual semi-quantitative analysis and carry out quantitative detection through ultraviolet rays, and the universality of the sensor can be confirmed through a template replacement experiment. And meanwhile, the sensor shows an excellent cost advantage. The diagnosis which is high in sensitivity, low in cost and capable of achieving rapid visualization shows huge potential in practical application, and particularly has important application value in infectious disease screening and monitoring work in areas with limited resources.

The invention relates to the technical field of biological separation materials, and discloses an amine-functionalized magnetic nano composite material and application thereof in protein separation and purification. The material has a core-shell structure and comprises a magnetic ferroferric oxide nano core, an ordered mesoporous silicon dioxide intermediate layer and a covalent grafted polyethyleneimine functional layer. By controlling the matching relationship between the pore diameter of mesopores and the molecular size of the polymer and adopting a crosslinking strategy, the material has high magnetic responsiveness, rapid mass transfer performance and high-density surface amino groups. The material shows high adsorption capacity, rapid adsorption kinetics and good cycle stability in the protein separation process, and is suitable for enrichment purification and metal chelating affinity separation of protein in a biological sample. According to the invention, the technical problems of low protein adsorption capacity, slow dynamics and poor cycling stability caused by compact shell layer, non-uniform amino distribution and limited mass transfer of the existing amine functionalized magnetic material are solved.

The present invention discloses a magnetic nanocomposite material, a preparation method thereof, and an application thereof in the enrichment of low-abundance proteins. The magnetic nanocomposite material proposed by the present invention is Fe3O4@TS-1. Its preparation method is simple to operate. The sol-gel hydrothermal method is adopted, and the preparation is stable. The magnetic composite material Fe3O4@TS-1 is successfully synthesized. While ensuring the material properties of the TS-1 molecular sieve material, this material not only guarantees excellent protein adsorption performance but also endows it with magnetism, providing a simpler, faster, and more efficient technical method for large-scale sample processing. The enrichment of low-abundance proteins based on the magnetic nanocomposite material proposed by the present invention can be applied to almost all sample types, effectively solving the interference caused by high-abundance proteins to the identification of low-abundance proteins during mass spectrometry detection, and the number of protein identifications can be increased by 100% - 700%.

The invention belongs to the technical field of biological pharmacy, and particularly relates to a preparation method and application of a nano-drug carrier. The invention provides a preparation process based on a pre-polymerization-coordination-shaping sequence, which comprises the following steps: firstly, slightly combining chitosan and polyphenol under a near-neutral condition to form small particles with uniform size and good dispersity, so that adhesion among the particles is effectively reduced; then ferric ions are slowly introduced under the regulation and control of a weak chelating agent, so that the ferric ions are uniformly coordinated on the surfaces of the particles, and an inner layer which is compact in structure and has responsiveness to an acid environment or a competitive ligand is constructed; and finally, sodium alginate is introduced, crosslinking is performed through slow-release calcium ions, a flexible outer layer is formed, and the layer and the inner layer have a synergistic effect, so that the dilution resistance and the protein adsorption resistance of the carrier are remarkably enhanced.

The invention relates to the technical field of medical materials, in particular to a self-lubricating PEBAX / TPU material suitable for a medical catheter and a preparation process of the self-lubricating PEBAX / TPU material. 8-15 parts of a self-lubricating modified material; 1.5 to 3 parts of an antioxidant complexing agent; and 3-5 parts of a compatilizer. According to the self-lubricating PEBAX / TPU material, fluorocarbon chains are enriched on the surface to form a long-acting low-friction layer through fluorinated polyethylene and hydrophobic nano silicon dioxide, an external coating is not needed, an antioxidant Irganox 1010 and carbodiimide synergistically inhibit oxidation and hydrolysis, the chemical stability is remarkably improved, the compatibility of PEBAX / TPU is improved through maleic anhydride grafted POE, and the self-lubricating PEBAX / TPU material has the advantages that the self-lubricating performance is good; the rigid gradient of the conduit is optimized by combining a multi-layer co-extrusion process, the protein adsorption resistance of the surface is further enhanced through plasma treatment and perfluorosilane grafting, the integrity of the material is guaranteed through accurately controlled low-temperature extrusion and low-dose gamma sterilization, and the material is more stable and reliable while the biological safety is maintained. The synergistic improvement of the lubrication durability, the mechanical property and the aging resistance is realized.

Provided is a technique for improving the efficiency of adsorption of a protein onto a carrier in an apparatus equipped with an in-line mixer and a hydrocyclone. An adsorption method disclosed herein is performed using an apparatus equipped with an in-line mixer and a hydrocyclone connected to the in-line mixer. The adsorption method is a method in which a protein is adsorbed onto a carrier provided with a ligand capable of binding to the protein. The adsorption method includes: mixing a first slurry containing a carrier with a second slurry containing a protein using an in-line mixer to prepare a mixed slurry; and adsorbing the protein onto the carrier in the mixed slurry to produce a complex between the protein and the carrier. The mixed slurry is prepared so as to contain the carrier in an amount of 10-25 vol% and the protein in an amount of 5-20 mg per 1 ml of the carrier when the whole amount of the mixed slurry is 100 vol%. The mixing time in the inline mixer is 0.6-60 seconds.

A method for purifying a protein comprising an antibody, antibody fragment, or immunoglobulin single variable domain, from a solution containing at least one contaminant by superantigen chromatography comprising: a) adsorbing the protein to the superantigen immobilized on a solid support; b) removing the at least one contaminant by contacting the immobilized superantigen containing the adsorbed protein with a first wash buffer comprising an aliphatic carboxylate; and c) eluting the protein from the superantigen immobilized on the solid support.

The present invention discloses a polyethersulfone membrane with low protein adsorption function and its preparation method. The polyethersulfone membrane comprises the following raw materials according to mass percentage: 10-30% of polyethersulfone, 3-15% of amphiphilic copolymer, 2-10% of pore former, 0.5-15% of non-solvent, and 55-75% of solvent. Through a carefully designed formulation and preparation process, the present invention successfully prepares a polyethersulfone membrane with low protein adsorption function. The addition of the amphiphilic copolymer P(PVP-r-PDMS) not only enhances the hydrophilicity and protein adsorption resistance of the membrane, but also realizes the optimization of the membrane structure through the fine regulation of the RTIPS method. Finally, a polyethersulfone hollow fiber membrane with excellent anti-fouling performance and high permeability is obtained, which has broad application prospects in the fields of hemodialysis, drug filtration, food industry, etc.

The invention relates to the technical field of blood purification materials, in particular to a zirconium-titanium bimetal MOF hollow microsphere adsorbent and a preparation method and application thereof. The adsorbent is based on a Zr / Ti bimetallic node and a phosphonic acid-carboxylic acid mixed ligand, a composite MOF hollow microsphere structure with high stability and high specific surface area is constructed through a solvothermal and polymerization synergistic reaction in a water-in-oil suspension system, a phosphonic acid group and Zr are strongly coordinated to improve the stability of a framework, and the composite MOF hollow microsphere structure has a high specific surface area. The carboxylic acid group keeps the hydrophilicity of the pore channel; meanwhile, a cross-linked network of acrylamide and N, N '-methylene bisacrylamide penetrates through crystal gaps in the balling process, so that the material has excellent mechanical strength and fluid stability; and a sulphobetaine amphoteric polymer brush layer is constructed on the surface of the MOF through endogenous initiation point modification and surface in-situ controllable free radical polymerization technologies, so that protein adsorption and platelet adhesion are effectively inhibited, the blood compatibility is remarkably improved, and meanwhile, the channel accessibility is maintained to promote rapid diffusion and adsorption of bilirubin.

The invention relates to the technical field of flexible bioelectronics and biomedical sensors, in particular to a biocompatible flexible carbon-based physiological electrode prepared through coaxial spinning and application of the biocompatible flexible carbon-based physiological electrode. The conductive core is formed by crosslinking graphene oxide, carbon nanotubes and the like; the buffer layer contains polyurethane and the like, and shape memory microspheres and antibacterial nano-silver are added; a biological interface layer contains chitosan and the like, and PEG-PE is grafted to reduce protein adsorption; the thickness ratio of the three layers is 1: (1.5-2): (0.5-1), and the thickness is 100-300 microns; the electrode has conductive, sensing and antibacterial functions, can load double enzymes for blood glucose monitoring, is strong in interlayer bonding, and is suitable for various biomedical scenes. By innovating the three-layer structure and the material design, high conductivity, high flexibility and excellent biocompatibility are achieved; multi-parameter monitoring and electrical stimulation treatment can be achieved, the anti-bending performance is good, the antibacterial and anticoagulant effects are good, the preparation process is controllable, and the application prospects in the fields of wearable equipment, implantation medical treatment and the like are wide.

The invention relates to the technical field of oral biological materials, in particular to a self-adaptive anti-caries coating as well as a preparation method and application thereof. The coating is prepared from random and block polymers, and is specifically prepared by polymerizing acid radical-containing functional monomers, phenylboronic acid and derivatives thereof. Construction of the coating depends on specific coordination of a functional group and Ca < 2 + > in enamel, and stable anchoring on the tooth surface is achieved. The core action mechanism is to introduce phenylboronic acid groups to endow the coating with pH sensitive characteristics: in a cariogenic acid environment, biofilm formation is synergistically inhibited through a plurality of mechanisms such as physical barrier, electrostatic repulsion and inhibition of mucoprotein-mediated adhesion. According to the application, the Ca < 2 + > anchoring monomer, phenylboronic acid and the derivative thereof are copolymerized, design is carried out aiming at two key links of protein adsorption and bacterial adhesion, multi-mechanism synergy and environment dynamic response are integrated, and an integrated anti-caries effect from early intervention to long-term protection is realized.

This invention discloses a magnetic nano-silica, its preparation method, and its application in the enrichment of low-abundance proteins. The proposed method for preparing magnetic silica, while preserving the surface properties of silica and maintaining its protein adsorption capacity, also imparts magnetism; providing a simpler, faster, and more efficient technique for large-scale sample processing. The low-abundance protein enrichment based on magnetic nano-silica proposed in this invention can be applied to almost all sample types, effectively solving the interference caused by high-abundance proteins on the identification of low-abundance proteins during mass spectrometry detection, and increasing the number of identified proteins by 100%-700%.

This invention discloses a magnetic nanomolecular sieve, its preparation method, and its application in the enrichment of low-abundance proteins. The preparation method of the magnetic nanomolecular sieve proposed in this invention is simple to operate, employing a one-pot method. It simultaneously imparts magnetism while maintaining the surface properties of the sieve, i.e., its protein adsorption capacity, thus preserving its magnetic properties. This provides a simpler, faster, and more efficient technical method for large-scale sample processing. The low-abundance protein enrichment based on magnetic molecular sieves proposed in this invention can be applied to almost all sample types, effectively solving the interference caused by high-abundance proteins on the identification of low-abundance proteins during mass spectrometry detection, and increasing the number of protein identifications by 100%-700%.

A method for purifying a protein comprising an antibody, antibody fragment, or immunoglobulin single variable domain, from a solution containing at least one contaminant by superantigen chromatography comprising: a) adsorbing the protein to the superantigen immobilized on a solid support; b) removing the at least one contaminant by contacting the immobilized superantigen containing the adsorbed protein with a first wash buffer comprising an aliphatic carboxylate; and c) eluting the protein from the superantigen immobilized on the solid support.

This invention relates to the field of medical device technology and provides a method for preparing a drug sustained-release coating and a medical device containing the same. The method includes forming a drug sustained-release coating on the surface of the medical device using a drug sustained-release polymer and a drug. The drug sustained-release polymer is a multi-component polymer containing functional groups for anti-protein adsorption, drug sustained-release, and chemical cross-linking. While ensuring the anticoagulant properties and adhesion to the surface of the medical device, the drug sustained-release coating, through the synergistic effect of butyl methacrylate and trimethoxysilyl propyl methacrylate, achieves an initial burst release of less than 20% of the total drug amount and slows down drug release to ensure a drug release cycle in vivo of no less than 21 days. This drug sustained-release coating can release a stable amount of drug to the tissue wall it contacts over a long period, aiming to achieve a long-term therapeutic or preventative effect on diseases such as luminal stenosis.

To provide a protein adsorption inhibitor that can suppress the adsorption of proteins to surfaces such as immunoreaction vessels and measuring instruments to a high degree, while also reducing variability between measurements. [Solution] Formula (1): Z-{O-[(PO) a (EO) b ]-(AO) c -H]} x A protein adsorption inhibitor comprising an alkylene oxide derivative represented by (the definition of the symbols in the formula is as described in the specification), wherein the cloud point of a 1% by mass aqueous solution of the alkylene oxide derivative is 0°C or higher and 30°C or lower.

The application belongs to the technical field of medical biomaterials, and provides a natural polysaccharide-polyphenol network polytetrafluoroethylene artificial blood vessel and a preparation method, wherein the natural polysaccharide-polyphenol network polytetrafluoroethylene artificial blood vessel comprises an expanded polytetrafluoroethylene artificial blood vessel base body, further comprises a polydopamine-acetone copolymerization intermediate layer attached to the whole surface of the base body, and a natural polysaccharide-polyphenol network coating layer covalently crosslinked to the inner surface of the intermediate layer; the natural polysaccharide-polyphenol network coating layer is formed by tannic acid as a natural crosslinking agent and sulfonated carboxymethyl chitosan through covalent bonds, hydrogen bonds and hydrophobic interaction; the outer surface of the base body is rich in methyl propionyl groups and retains hydrophobicity; the application has excellent anticoagulant properties, good biological safety, selective functionalization of the inner and outer surfaces, excellent anti-protein adsorption properties and good coating stability, and has a simple preparation process and low cost.

The invention belongs to the technical field of material surface performance detection, and particularly relates to a method for detecting biological pollution resistance and durability of a silicone rubber surface, and the method comprises the following steps: S1, sequentially carrying out biological membrane inhibition rate quantitative detection, antibacterial adhesion detection, protein adsorption resistance test and surface wettability test on a silicone rubber sample to be detected; s2, carrying out durability treatment on the parallel sample; s3, repeating partial or all tests in the step S1 on the sample treated in the step S2; s4, comparing the performance data obtained in the step S1 and the performance data obtained in the step S3, and evaluating the biological pollution resistance and durability of the silicone rubber sample. According to the invention, biological membrane inhibition rate quantitative detection, anti-bacterial adhesion detection, anti-protein adsorption performance test, surface wettability analysis and durability test are organically combined to form a complete evaluation closed loop from molecular adsorption to microbial community and from immediate efficiency to long-acting stability, and all key links of biological pollution are comprehensively covered.

This invention relates to a hyperbranched polylysine-modified macroporous chromatographic medium, its preparation method, and its application, belonging to the field of chromatographic separation technology. The preparation method includes the following steps: dispersing macroporous polysaccharide microspheres in a solvent to obtain a medium suspension; subsequently adding epichlorohydrin to the medium suspension and mixing thoroughly; adding sodium hydroxide solution to the medium suspension and stirring to obtain an epoxidized macroporous polysaccharide medium; immersing the epoxidized macroporous polysaccharide medium in a hyperbranched polylysine solution and stirring to ensure sufficient diffusion of the hyperbranched polylysine within the microspheres; the hyperbranched polylysine does not contain any hydrophobic groups; adding sodium hydroxide solution and sodium borohydride solution to the system and stirring to obtain the hyperbranched polylysine-modified macroporous chromatographic medium. Compared with existing technologies, this invention utilizes hyperbranched polylysine to modify macroporous chromatographic media, which can significantly improve protein adsorption capacity and adsorption rate.

To provide a method for adsorbing dropped protein to a base material having a titanium oxide layer on the surface at a high rate.SOLUTION: A method for adsorbing a protein to a substrate having a titanium oxide layer on a surface thereof, the method comprising: a protein solution dropping step of dropping a protein solution containing a protein onto the titanium oxide layer of the substrate; and a protein adsorption step of adsorbing the protein contained in the dropped protein solution onto the titanium oxide layer, wherein the protein solution contains 0.1 mg to 5 mg / mL of bovine serum albumin, and the substrate onto which the protein solution has been dropped is held for 85 minutes to 480 minutes in the protein adsorption step.SELECTED DRAWING: Figure 2

The invention provides a preparation method and application of a high-flux blood-compatible polyimide separation membrane, polyimide polymer powder is added into a solvent B, a quaternization reagent is added to obtain quaternization polyimide polymer powder, the quaternization polyimide polymer powder is added into a solvent C, and the high-flux blood-compatible polyimide separation membrane is obtained. And scraping the membrane casting solution by using a scraper to prepare an asymmetric separation membrane, and washing to obtain the high-flux blood-compatible polyimide separation membrane. The separation membrane applied to the field of biological water treatment is prepared through chemical imidization condensation reaction and quaternization reaction. The high-flux blood-compatible polyimide separation membrane obtained by the invention has excellent thermal stability, good mechanical properties, protein adsorption resistance, bacterial adhesion resistance, blood compatibility and anticoagulation capability. The method has wide application prospects in the fields of ultrafiltration, hemodialysis, purification, biological sewage treatment and the like.

The present invention provides compositions and methods for reducing protein loss during drug delivery caused by protein adsorption onto one or more drug delivery system components. [Solution] In some embodiments, the present disclosure provides a composition for preventing the adsorption of proteins to one or more drug delivery system components, the composition comprising succinate and polysorbate 80. In some embodiments, the composition further comprises a therapeutic protein.

The application relates to the field of blood purification materials, and particularly discloses an anion exchange resin membrane applied to blood purification and a preparation method thereof. The anion exchange resin membrane applied to blood purification is prepared by blending a zwitterion-anion exchange copolymer and a hydrophobic film-forming polymer; the zwitterion-anion exchange copolymer is copolymerized by an anion exchange monomer and a zwitterion monomer, so that the resin membrane has anion exchange function and anti-protein adsorption function; the preparation method is as follows: the anion exchange monomer and the zwitterion monomer are subjected to free radical copolymerization under the action of an initiator; the copolymer and polyether sulfone are dissolved in a polar aprotic solvent to prepare a casting solution, and after casting film formation, phase inversion is completed by immersing in a coagulation bath; the obtained membrane is cleaned and subjected to alkalization treatment. The application solves the problems that traditional anion exchange resins easily cause non-specific protein adsorption, platelet adhesion and complement system activation.

Disclosed herein is a surfactant additive, ethylenediamine tetrakis(ethoxylate-block-propoxylate) tetrol with 16 ethylene oxide repeat units and 18 propylene oxide repeat units (known by its trade name as Tetronic 90R4) used as a droplet-additive, or to coat DMF driving electrode surfaces which dramatically improves the capability to work with high-protein-content liquids (e.g., whole blood) on digital microfluidic chips. This surfactant prevents protein adsorption and fouling of DMF electrode surfaces to an extent that was heretofore impossible. Specifically, this surfactant allows for the manipulation of droplets of undiluted whole blood for >1 hour per electrode (>1 50 times better than what is possible for any known additive). This improvement in handling high protein content media will revolutionize blood-based diagnostics on digital microfluidic platforms.

The invention provides an engineering exosome drug delivery system for over-expression of CD24 and a preparation method thereof, and belongs to the technical field of gene engineering. CD24 is overexpressed on a membrane of the engineered exosome, and a gene sequence of a plasmid of the overexpressed CD24 is as shown in SEQ ID No. 1; a CD24 sequence is fused to the N end of an I-type transmembrane protein structure LAMP2B on a membrane of the engineered exosome, a glycosylation sequence GNSTM is introduced to the N end of the fusion sequence, and an exosome binding peptide Signal peptide fragment is fused to the N end of the glycosylation sequence GNSTM. The engineering exosome for over-expressing CD24 on an exosome membrane is successfully constructed by using a fusion gene for coding transmembrane proteins LAMP2B and CD24, the aggregation and protein adsorption phenomena of the engineering exosome can be effectively retarded, the drug delivery targeting of the engineering exosome is enhanced, the in-vivo duration of the engineering exosome is prolonged, and the delivery performance of the engineering exosome is further improved.

The present application relates to the use of polysialic acid or its derivatives in anti-foreign body reaction, in particular, to a polysialic acid anti-foreign body reaction material for resisting biological contamination, for regulating macrophage phenotype to reduce inflammatory response, and for resisting foreign body reaction. The polysialic acid material includes a bulk material prepared from polysialic acid and its derivatives, or a coating material for surface modification of the material, which can resist protein adsorption, cell adhesion and adhesion of various microorganisms, can act on immune cells, such as regulating the phenotype of macrophages, thereby reducing acute and chronic inflammatory response, and has excellent anti-foreign body reaction effect as an implant material.

The invention belongs to the technical field of biological materials, and particularly discloses a construction method of an anti-protein adsorption and antithrombotic graphene oxide coating, which comprises the following steps: step 1, carboxylating graphene oxide to obtain carboxylated graphene oxide nanoparticles; step 2, grafting heparin to the carboxylated graphene oxide nanoparticles; step 3, the graphene oxide-heparin nanoparticles are combined with polyethylene glycol, and graphene oxide-heparin-polyethylene glycol nanoparticles are obtained; and step 4, combining the graphene oxide-heparin-polyethylene glycol nanoparticles with a biological material. The preparation method has the advantages that the biocompatibility of the medical cardiovascular biological material is improved, the protein adsorption resistance and the thrombus resistance of the medical cardiovascular biological material are improved, and the in-vivo stability of the biological material is improved.

The invention provides a Pt / PEDOT composite modified carbon-based material needle-shaped electrode in-vivo dissolved oxygen electrochemical sensor as well as a construction method and application thereof, and belongs to the technical field of medical biology. According to the in-vivo dissolved oxygen electrochemical sensor, a carbon-based material microneedle type electrode is modified by electrochemical deposition of platinum nanoparticles by using a chloroplatinic acid hexahydrate solution and depending on a time current method, and poly (3, 4-ethylenedioxythiophene) is generated by direct electro-polymerization on the surface of the Pt-modified microneedle electrode through an electrochemical constant current method. And forming uniform PEDOT (poly (3, 4-ethylenedioxythiophene)) film coated and modified platinum nanoparticles on the microneedle electrode. The composite modified carbon-based material is used for replacing precious metal to reduce cost and improve biocompatibility, low-oxygen sensitivity is optimized by means of composite modification, in-vivo protein adsorption and biological membrane coverage are resisted through anti-pollution surface modification, and therefore the defects are overcome, and monitoring of in-vivo tissue oxygen partial pressure is achieved.

The invention relates to acrylic acid self-polishing antifouling resin capable of being stripped in a layered manner in seawater as well as a preparation method and application of the acrylic acid self-polishing antifouling resin. The preparation method of the antifouling resin comprises the steps of preparing a macromolecular acrylic acid organosilicon monomer, preparing a monomer mixture containing an initiator and the like. The acrylic acid self-polishing antifouling resin capable of being stripped in the seawater in a layered manner, which is prepared by the preparation method disclosed by the invention, shows good protein adsorption resistance and antibacterial growth resistance, and can be stripped in the seawater in a layered manner, so that stains adhered to the surface of a coating are released into a marine environment along with stripping of the coating; the surface of the new coating still can show an antifouling behavior, so that the new coating shows excellent antifouling capacity in actual ocean. The self-polishing antifouling resin is simple in preparation process and low in raw material cost, and has large-scale production and application prospects.

The invention discloses a preparation method of a silk conical artificial blood vessel with anticoagulation / endothelialization promotion functions, which comprises the following steps: preparing an artificial blood vessel for intracavity isolation through silk and polyester yarns, and successfully constructing a phosphorylcholine / heparin bionic functional coating on the surface of the blood vessel by using dopamine hydrochloride and hexamethylenediamine. According to the method, phosphorylcholine / heparin is combined with surface coating modification, and a novel intracavitary isolated blood vessel with excellent biological functions and mechanical properties is constructed. The structure of the artificial blood vessel is more matched with the shape of the inner wall of the blood vessel, blood flow distribution and tissue regeneration are facilitated, the anticoagulant property and endothelialization ability of the artificial blood vessel are effectively improved through the phosphorylcholine / heparin coating, protein adsorption and platelet activation can be remarkably reduced, the blood coagulation cascade reaction can be blocked, endothelial cell adhesion and proliferation can be accelerated, and the risk of acute thrombus after operation can be reduced. The method provides a new technical path for design and functionalization of the conical artificial blood vessel in the intracavitary isolation operation, and has important clinical application prospects.