Method for forming simulated cell outer layer membrane structure on surface of cross-linked chitosan

A technology of imitating cell outer film and cross-linked chitosan, which is applied in the field of surface science and biomedical polymer materials, can solve the problems of low density of phosphorylcholine groups, and achieves inhibition of protein adsorption and high graft density. , the effect of simple preparation process

Inactive Publication Date: 2009-09-16
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the phosphorylcholine group density on the surface of the current modified chitosan is low, and when the density is high, it will dissolve in contact with body fluids, thus limiting its application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0019] Preparation of cross-linked chitosan film: dissolving chitosan with 1% (v / v) acetic acid to obtain a 0.1-0.8 wt% chitosan solution. After filtering the above-mentioned chitosan solution, let it stand for defoaming, and add 5% glutaraldehyde to carry out cross-linking. After stirring overnight at room temperature, a light yellow cross-linked chitosan solution was obtained. The cleaned glass slide is vertically immersed in the cross-linked chitosan solution, and after the cross-linked chitosan solution immerses the glass slide, gently lift the glass slide. The slides coated with the cross-linked chitosan membrane were allowed to dry at room temperature. Soak the cross-linked chitosan membrane with 0.5wt% sodium hydroxide solution for 1.5h, then fully wash it with double distilled water until the conductivity of the washing water is equivalent to that of distilled water, and finally dry it under vacuum at 30°C.

[0020] Dichlorophosphorylcholine grafted cross-linked chit...

Embodiment 1

[0024] Dissolve 0.5g of chitosan in 100mL of 1% acetic acid aqueous solution, stir at room temperature for 20 hours, then filter with a sand-core funnel, and let stand to eliminate air bubbles. Take 50 mL of the filtered solution, add 50 μL of 5% glutaraldehyde, stir at room temperature for 8 hours, apply it on a clean glass slide, and let it air for 24 hours at room temperature. Place the dried chitosan film in 0.1% sodium hydroxide solution to soak for 0.5 hours, and then soak in distilled water for 40 hours. Finally, vacuum-dry at 30°C for 8 hours to obtain a cross-linked chitosan film.

Embodiment 2

[0026] Dissolve 1 g of chitosan in 100 mL of 2% acetic acid aqueous solution, stir at room temperature for 20 hours, then filter with a sand funnel, let stand to eliminate air bubbles, and set aside. Add 100mL carbon tetrachloride, 100mL petroleum ether, and 10mL span-80 into a 500mL three-necked bottle, set the speed at 700, stir at room temperature for 0.5 hours, then add the chitosan solution above and stir for 0.5 hours, then add 5% 2 mL of glutaraldehyde was reacted for 1.5 hours, then adjusted to pH=8-9 with 10% sodium hydroxide, then reacted at 40°C for 3 hours and at 60°C for 4 hours. Finally, vacuum-dry at 50° C. for 6 hours to obtain cross-linked chitosan particles.

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PUM

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Abstract

The invention discloses a method for forming a simulated cell outer layer membrane structure on the surface of cross-linked chitosan. The method comprises the following steps that: firstly, the chitosan is cross-linked by glutaraldehyde; and secondly, the surface of the cross-linked chitosan is grafted with phosphinylideyne choline groups to construct the simulated cell membrane structure, the reaction of the grafted phosphinylideyne choline groups is carried out in an anhydrous non-protonic solvent, the reaction temperature is between 40 and 80 DEG C, and the concentration of phosphorylcholine dichloride is between 0.1 and 20mg/ml. The test results show that for the surface modified by the simulated cell outer layer membrane structure, the capabilities of adsorbing protein and blood platelet are obviously reduced, and the blood compatibility is obviously improved. The modified material with the simulated cell outer layer membrane structure has wide application prospect in fields such as blood purification, body implantation materials, tissue engineering, slow release of medicines, biological sensors, and the like.

Description

technical field [0001] The invention relates to the construction of a cross-linked chitosan surface imitation cell membrane structure and its application as biological and medical materials, in particular to a modified cross-linked chitosan material, which belongs to the technical fields of surface science and biomedical polymer materials. Background technique [0002] When the biomedical material is used in the body, the material will coagulate and form a thrombus after contact with blood; contact with tissue will generally cause inflammation. There is currently no synthetic or natural medical biomaterial that can fully meet the biocompatibility requirements. Therefore, selecting existing materials with good physical properties for biocompatible surface modification has become the focus of research. [0003] As the only alkaline polysaccharide that exists in large quantities in nature, chitosan is a non-toxic, non-polluting renewable resource with good biocompatibility and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F251/00
Inventor 宫铭皇甫蓬勃杨姗宫永宽
Owner NORTHWEST UNIV(CN)
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