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1350 results about "Cell membrane" patented technology

The cell membrane (also known as the plasma membrane (PM) or cytoplasmic membrane, and historically referred to as the plasmalemma) is a biological membrane that separates the interior of all cells from the outside environment (the extracellular space) which protects the cell from its environment. Cell membrane is consisted of a lipid bilayer, including cholesterols (a lipid component) that sit between phospholipids to maintain their fluidity under various temperature, in combination with proteins such as integral proteins, and peripheral proteins that go across inside and outside of the membrane serving as membrane transporter, and loosely attached to the outer (peripheral) side of the cell membrane acting as several kinds of enzymes shaping the cell , respectively. The cell membrane controls the movement of substances in and out of cells and organelles. In this way, it is selectively permeable to ions and organic molecules. In addition, cell membranes are involved in a variety of cellular processes such as cell adhesion, ion conductivity and cell signalling and serve as the attachment surface for several extracellular structures, including the cell wall, the carbohydrate layer called the glycocalyx, and the intracellular network of protein fibers called the cytoskeleton. In the field of synthetic biology, cell membranes can be artificially reassembled.

All-optical three-dimensional scanning confocal fluorescence microscopic imaging device and implementation method thereof

The invention discloses an all-optical three-dimensional scanning confocal fluorescent microscopic imaging device and an implementation method thereof. According to the invention, through a deep learning driven adaptive regulation and control method, CNN is adopted to process spatial distribution data and dynamically regulate and control a phase hologram and the light intensity and phase compensation of a laser, so that the focal point of exciting light is subjected to aberration-free axial displacement, a bidirectional parallel optical scanning track of a two-dimensional scanning system is optimized, and all-optical three-dimensional scanning is realized; lSTM and TCN are combined to obtain a time sequence dependency relationship, a laser light source, an adjustable diaphragm, an electric focus-adjustable lens and a photoelectric detector are integrally controlled, efficient synchronization and automatic operation and high-speed axial focusing adjustment are realized, errors and time sequence mismatch are eliminated, optical characteristics of different samples and environmental interference are automatically adapted, and high-quality imaging is kept. The robustness and the applicable scene range of the system are improved; the method is used for model biological embryo real-time tracking, intracellular signal molecule dynamic visualization, cell membrane protein migration and aggregation observation and intracellular organelle interaction tracking.
Owner:PEKING UNIV

Curcumin-loaded MMP response type melittin nano-pellicle vesicle as well as preparation method and application of curcumin-loaded MMP response type melittin nano-pellicle vesicle

PendingCN121868251ABacteriaAntibody mimetics/scaffoldsCalcium phosphate coatingCell membrane
The invention relates to a curcumin-loaded MMP response type melittin nano-pellicle vesicle as well as a preparation method and application thereof, and belongs to the technical field of pharmaceutical preparations. The preparation method comprises the following steps: firstly, preparing curcumin entrapped lipidosome; then carrying out culture amplification on engineering bacteria carrying melittin recombinant plasmids, extracting cell membranes after removing cell walls, and carrying out ultrasonic treatment and membrane extrusion to obtain melittin cell membrane nano-vesicles; fusing the curcumin lipidosome and the cell membrane nano-vesicles in an ultrasonic extrusion mode to obtain fused vesicles; and finally, carrying out surface calcium phosphate mineralization treatment on the fused vesicles to obtain the curcumin-loaded MMP response type melittin nano pellicle vesicles. The nano mycofilm vesicle prepared by the invention can be used for preparing antitumor drugs, and the biocompatibility and in-vivo stability of nanoparticles are improved by introducing a calcium phosphate coating; through combined delivery of melittin and curcumin, the anti-tumor effect is enhanced, so that the growth and proliferation of tumor cells are inhibited.
Owner:DALIAN UNIV OF TECH

M2M-SeMSN-coated C176 nanoparticles, and preparation method and application thereof

The invention discloses an M2M-SeMSN-coated C176 nano-particle, a preparation method and application thereof, the nano-particle comprises an STING inhibitor C176 and a carrier, and the carrier is based on an M2 macrophage membrane and a selenium-bridged mesoporous silica nano-particle. Specifically, the M2M-SeMSN-coated C176 nano-particles are obtained by loading an STING inhibitor C176 by using a selenium-bridged mesoporous silica nano-particle SeMSN and M2 macrophage cell membrane. The invention further discloses a preparation method of the M2M-SeMSN-coated C176 nano-particles. The M2M-SeMSN-coated C176 nanoparticles can be used for preparing a medicine for treating acute kidney injury.
Owner:THE 953RD ARMY HOSPITAL OF THE CHINESE PEOPLES LIBERATION ARMY

Recombinant protein for detecting para-tumor Yo antibody through CBA method and application

ActiveCN121135895ABiological testingFermentationAntigenHuman albumin
The invention discloses a recombinant protein for detecting a para-tumor Yo antibody through a CBA method and application, and belongs to the technical field of biomedical engineering.The amino acid sequence of the recombinant protein sequentially comprises a secretory signal peptide, a CDR2 protein partial sequence, a CDR2L protein partial sequence, a transmembrane region and a fluorescent label, and the secretory signal peptide is human albumin signal peptide ALB; the transmembrane region is a CD8a hinge; and the fluorescent label is mCherry. A novel recombinant protein which can be stably over-expressed on a cell membrane of an eukaryotic cell is constructed by intercepting specific partial sequences of CDR2 protein and CDR2L protein and redesigning and fusing a fluorescent label by using a secretory signal peptide, a transmembrane sequence and a connecting peptide, and the recombinant protein retains respective core antigen regions of the CDR2 protein and the CDR2L protein, so that the specific partial sequences of the CDR2 protein and the CDR2L protein can be stably over-expressed on the cell membrane of the eukaryotic cell. The kit can effectively overcome the defects in the aspects of sensitivity and specificity, and when a CBA method is adopted for detection, the detection rate of the para-tumor Yo antibody can be remarkably increased, and the false positive rate is reduced, so that the requirements of clinical detection are better met.
Owner:CHENGDU HAIERYUNYIN MEDICAL LAB CO LTD

Sheep colostrum and polypeptide and preparation method thereof

ActiveCN121652236AMilk preparationMicroorganism based processesCell membraneHuman respiratory virus
The invention provides a polypeptide, and the sequence of the polypeptide is AEDVGDVAFVKNDT. The polypeptide provided by the invention can inhibit the human respiratory syncytial virus surface protein F so as to inhibit the fusion of the human respiratory syncytial virus surface protein F and a host cell membrane. The invention also provides the sheep colostrum. The sheep colostrum comprises the polypeptide with the antiviral function, so that the sheep colostrum is helpful for old people with relatively weak resistance to resist infection of human respiratory syncytial viruses and promotes repair of respiratory mucosa. The invention also provides a preparation method of the sheep colostrum. The preparation method is simple and can be used for industrial production. The invention also provides a preparation method of the polypeptide.
Owner:HUNAN NUTRITION TREE BIOTECHNOLOGY CO LTD

Drug-loaded nano vesicle as well as preparation method and application thereof

The invention belongs to the field of biological medicines, and relates to a drug-loaded nano-vesicle as well as a preparation method and application thereof. The drug-loaded nano-vesicle comprises a vesicle core and a drug-loaded nano-vesicle, wherein the vesicle core comprises siRNA (small interfering Ribonucleic Acid) capable of specifically targeting and silencing an NR1D1 gene; the vesicle membrane is formed by fusing an erythrocyte membrane, a macrophage membrane, cardiolipin, cholesterol and lecithin. The drug-loaded nano-vesicle can specifically target macrophages in a sepsis immunosuppression stage, has an intracellular response release function, recovers BMAL1 and IGF2BP2-ATP6V1B2 / ATP6V0c axis functions by inhibiting NR1D1 expression, reconstructs a macrophage phagocytosis function and lysosome-dependent bacterium removal capability, and can be used for preparing a drug-loaded nano-vesicle with a specific targeting function. The survival rate of sepsis immunosuppression model animals is obviously improved; and the bacterial load is reduced. Compared with a traditional electroporation method, the preparation method disclosed by the invention has the advantage that the encapsulation efficiency of siRNA is remarkably improved.
Owner:XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV

Antibacterial peptide Mh-GC21 as well as precursor and application thereof

The invention belongs to the technical field of antibacterial peptides, and particularly relates to an antibacterial peptide Mh-GC21 as well as a precursor and application thereof. The amino acid sequence of the antibacterial peptide Mh-GC21 provided by the invention is as shown in SEQ ID NO.1, and two cysteines are oxidized to form a pair of intermolecular disulfide bonds; the C tail end of the antibacterial peptide Mh-GC21 is subjected to amidation modification. The antibacterial peptide Mh-GC21 provided by the invention is from microhyla microcarpa, has broad-spectrum antibacterial activity, has relatively good antibacterial and bactericidal effects on standard strains of acinetobacter baumannii, escherichia coli and staphylococcus aureus and clinically sourced drug-resistant strains, and can be used for targeted destruction of cell membranes of bacteria, removal of biological membranes and inhibition of formation of the biological membranes. Moreover, the antibacterial peptide Mh-GC21 has the advantages of good stability, no hemolytic activity and cytotoxicity, and difficulty in inducing strains to generate drug resistance.
Owner:GUANGDONG LABORATORY OF SOUTHERN OCEAN SCIENCE AND ENGINEERING (GUANGZHOU)

Recombinant protein for improving curative effect of ADC drug and application of recombinant protein

The invention relates to the technical field of biology, and particularly discloses a recombinant protein for improving the curative effect of an ADC drug and application of the recombinant protein. The recombinant protein comprises a tumor cell surface targeting structure, a cell transmembrane structure and toxin molecules, the toxin molecule is connected and fused with the tumor cell surface targeting structure and / or the cell transmembrane structure; the tumor cell surface targeting structure is a protein structure capable of being specifically combined with a tumor cell surface target spot; and the cell penetrating structure is a protein structure for mediating the recombinant protein to penetrate through a cell membrane to enter the cell. The tumor cell surface targeting structure is used for specifically recognizing a target cell surface target spot, the killing activity of a conventional antibody is brought into play, then toxin molecules are brought into tumor cells through the cell transmembrane structure, toxin is released, the tumor killing effect is achieved, the ADC drug curative effect is improved, and the ADC drug application prospect is wide. The transmembrane efficiency of the cell transmembrane structure can be improved to 50-90%, so that the traditional ADC drug treatment window is improved.
Owner:HEBEI SHENYU BIOTECHNOLOGY CO LTD

Rice purple acid phosphatase OsPAP16 and application thereof

The invention belongs to the technical field of gene engineering, and provides rice purple acid phosphatase OsPAP16 and application thereof, and the application is that a rice purple acid phosphatase OsPAP16 mutant is applied to rice cultivation. Two OsPAP16 mutants are prepared by adopting a gene editing technology, then the mutants are subjected to overexpression induction, and the result shows that the purple acid phosphatase OsPAP16 is positioned on a cell membrane, the OsPAP16 is subjected to phosphorus deficiency induced expression on leaves and roots, the phosphorus deficiency induced expression at the roots is quicker and higher, the overexpression of the OsPAP16 can improve the activity of the acid phosphatase on the root surface, and the yield of the acid phosphatase on the root surface is increased. Phosphorus deficiency stress of the rice can be relieved by degrading organic phosphorus in the environment, the phosphorus content of the rice is increased, and then growth and development of the rice are promoted.
Owner:HUAZHONG AGRI UNIV

Preparation method of artificial cell coated with macrophage membrane

ActiveCN121182744ABlood/immune system cellsPoly(diallyldimethylammonium chloride)Membrane technology
The invention discloses a preparation method of an artificial cell coated with a macrophage membrane, and belongs to the technical field of artificial cell preparation. The preparation method comprises the following steps: dissolving poly (diallyldimethylammonium chloride) and sulfobutyl-beta-cyclodextrin in ultrapure water, and mixing an aqueous solution of the poly (diallyldimethylammonium chloride) and an aqueous solution of the sulfobutyl-beta-cyclodextrin to obtain a PDDA / SBE-beta-CD condensation liquid droplet solution; and mixing the macrophage membrane suspension and the PDDA / SBE-beta-CD condensed fluid drops in an equal mass ratio, and realizing in-situ coating of the macrophage membrane by adopting an ultrasonic coating technology to obtain the artificial cell coated with the macrophage membrane. The PDDA / SBE-beta-CD condensation liquid droplet constructed by the invention has good stability under the conditions of physiological salt concentration and pH and at 37 DEG C; the artificial cell coated with the macrophage membrane is prepared by mixing with the macrophage membrane suspension, and the macrophage membrane serving as a shell not only can prevent aggregate aggregation, but also can improve the structural stability and biocompatibility.
Owner:NANCHANG UNIV

Solid dispersion as well as preparation method and application thereof

PendingCN121197063AOrganic active ingredientsPowder deliveryCell membranePhospholipid complex
The invention belongs to the technical field of medicines, and particularly provides a solid dispersion as well as a preparation method and application thereof. The solid dispersion comprises a carrier material, and further comprises quercetagetin and phospholipid, the mass ratio of the quercetagetin to the phospholipid is 1: (0.3-1.5), and the carrier material comprises one or more of a hydrophilic polymer and a pH-dependent polymer. Wherein the quercetagetin and the phospholipid are combined through an intermolecular force, so that the quercetagetin obtains a lipid material similar to a cell membrane structure, a cell membrane bionic phospholipid compound is formed, the cell membrane bionic phospholipid compound can be naturally compatible with an upper wall cell membrane of a small intestine, and the permeation transmembrane capability of the quercetagetin is better promoted; the quercetagetin is added into the solid dispersion, so that more quercetagetin can enter systemic circulation and lymphatic circulation, and therefore, the solid dispersion can strengthen transmembrane absorption while strengthening dissolution, so that the solid dispersion has high bioavailability.
Owner:CHENGUANG BIOTECH GRP CO LTD

M-coated PLGA-10BX compound as well as preparation method and application thereof

The invention belongs to the technical field of drug delivery, and particularly relates to an M-coated PLGA-10BX compound as well as a preparation method and application thereof. The preparation method comprises the following steps: preparing 10B-enriched boron nitride (h-10BN); extracting a cell membrane of the macrophage RAW264.7; polylactic acid-glycolic acid copolymer (PLGA) nano particles loaded with h-10BN are prepared; and finally, the bionic nano platform M (at) PLGA-10BN based on the macrophage membrane is prepared. The bionic nano-platform prepared by the invention is uniform in particle size and morphology, has relatively high biological safety, and has no obvious damage to various tissues and visceral organs. The compound can be used as a general platform for boron compound delivery, can also be used as an immune activator, is suitable for intravenous injection administration, and expands the application of boron neutron capture therapy (BNCT) in treatment of glioblastoma (GBM).
Owner:AFFILIATED HUSN HOSPITAL OF FUDAN UNIV

Biological organic fertilizer and preparation method thereof

The invention provides a biological organic fertilizer and a preparation method thereof. The biological organic fertilizer is prepared from the following raw materials in parts by weight: 10 to 15 parts of rice husk, 8 to 12 parts of peanut shell, 10 to 15 parts of bagasse, 15 to 20 parts of straw, 20 to 25 parts of livestock manure, 5 to 10 parts of pond sludge, 3 to 5 parts of plant ash, 5 to 10 parts of oil meal, 5 to 10 parts of ammonium phosphate, 2 to 5 parts of low-temperature complex microbial inoculants, 3 to 6 parts of seaweed oligosaccharide, 5 to 10 parts of modified vermiculite powder and 10 to 20 parts of photothermal conversion microspheres. According to the invention, waste is taken as a basic raw material and is matched with ammonium phosphate to rapidly supplement available nutrients, and meanwhile, the low-temperature complex microbial inoculant, the modified vermiculite powder and the photothermal conversion microspheres are introduced to form a synergistic interaction system. Vermiculite powder is modified, so that the nutrient adsorption and slow release capability is improved, a carbon source and low-temperature protection are provided for microorganisms, and the cell membrane stability is enhanced. The photothermal conversion microspheres efficiently absorb sunlight and convert the sunlight into heat energy, a local and mild heat island effect is formed around the fungicide, the microenvironment temperature is increased, breeding of the fungicide is remarkably accelerated, and therefore organic matter decomposition and nitrogen conversion are accelerated.
Owner:ZHEJIANG PUJIANG BOTAI ENVIRONMENTAL PROTECTION TECH CO LTD

EGFR targeted nano-drug carrier and preparation method thereof

The invention discloses an EGFR (epidermal growth factor receptor) targeted nano-drug carrier and a preparation method thereof, the EGFR targeted nano-drug carrier comprises drug-loading nanoparticles, an erythrocyte membrane coating the drug-loading nanoparticles and GE11 peptide connected to the erythrocyte membrane, and the drug-loading nanoparticles are formed by connecting tannic acid, ellagic acid and 1, 4-phenyldiboronic acid through boric acid ester bonds. The EGFR targeted nano-drug carrier disclosed by the invention can be enriched at an EGFR overexpressed tumor site in a targeted manner, and can be effectively prevented from being cleared by an immune system, and the drug loaded by the drug-loaded nanoparticles is released in a high-active oxygen environment, so that the treatment effect on tumors can be improved, and the toxic and side effects on normal tissues can be reduced; and photothermal therapy and chemotherapy effects can be simultaneously generated under NIR illumination, and the anticancer efficiency can be remarkably improved through the synergistic effect of the photothermal therapy and the chemotherapy.
Owner:CHONGQING UNIV CANCER HOSPITAL

Engineering bionic nucleic acid nano diagnosis and treatment agent as well as preparation method and application thereof

The invention discloses an engineered bionic nucleic acid nano diagnosis and treatment agent as well as a preparation method and application thereof, relates to the technical field of biological targeting, and aims to solve the problems that an engineered macrophage membrane modified bionic nucleic acid nano diagnosis and treatment agent for oral squamous cell carcinoma is lacked in the prior art, and the curative effect on invasive tumors of the oral squamous cell carcinoma is poor. According to the engineering bionic nucleic acid nano diagnosis and treatment agent, a cationic lipid nucleic acid medicine is wrapped with a macrophage membrane, and PD1 shown as SEQ.ID.NO.1 is stably expressed on the macrophage membrane; the cationic lipid nucleic acid medicine is prepared by loading Cip2a siRNA (small interfering Ribonucleic Acid) on a cationic liposome. The bionic nucleic acid nano diagnosis and treatment agent has a huge application prospect in preparation of oral squamous cell carcinoma diagnosis kits and medicines.
Owner:HARBIN MEDICAL UNIVERSITY

Immune sensitization IRE treatment platform based on NK cell membrane coated manganese carbonate

The invention relates to an immune sensitization IRE treatment platform based on NK cell membrane coated manganese carbonate. A manganese carbonate composite nano material is formed by coating the surfaces of manganese carbonate nano particles with cell membranes. According to the invention, higher tumor specificity enrichment is realized, non-target tissue accumulation and systemic toxicity risks are reduced, collaborative integration of tumor targeted delivery, immune activation and IRE ablation is realized, and compared with single IRE, anti-tumor immune response is significantly enhanced.
Owner:RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE

Metal polyphenol nano-enzyme as well as preparation method and application thereof

The invention relates to a metal polyphenol nano-enzyme, the metal polyphenol nano-enzyme comprises a core and a shell, the core comprises cerium, tannic acid and resveratrol, the shell comprises a hybrid cell membrane, and the shell wraps the surface of the core. The preparation method of the metal polyphenol nano enzyme comprises the following steps: preparing metal polyphenol nano particles; and extraction of a hybrid cell membrane and integration of a core and a shell. The metal polyphenol nano-enzyme provided by the invention has the advantages that the catalytic activity and selectivity are remarkably improved, the targeting capability and focus enrichment are improved, and controllable release and safety are realized.
Owner:GUANGZHOU CHUANGSAI BIOLOGICAL MEDICAL MATERIALS CO LTD

Preparation method of antibacterial modified polyester fiber

The invention relates to the technical field of polyester fibers, in particular to a preparation method of antibacterial modified polyester fibers, the prepared antibacterial modified polyester fibers have excellent antibacterial performance, a multi-layer antibacterial system is formed by introducing a nano-zinc oxide and Schiff base compound antibacterial agent into the polyester fibers, and the antibacterial performance of the polyester fibers is improved. Nano zinc oxide and copper / zinc oxide particles have broad-spectrum antibacterial property and can destroy cell walls and cell membranes of bacteria so as to achieve the antibacterial effect, and Schiff base as an organic antibacterial agent can be combined with receptors on the cell membranes of the bacteria to enhance the antibacterial effect; chitosan and cinnamyl aldehyde are adopted as raw materials for preparation of the Schiff base, and the two substances have good biocompatibility and degradability, so that the Schiff base compound antibacterial agent is safer and more environmentally friendly in the fiber; the nano zinc oxide, the copper / zinc oxide particles, the chitosan, the cinnamyl aldehyde and the like in the raw materials are all environment-friendly materials and are good in degradability, and environmental pollution and ecological damage can be reduced.
Owner:NANTONG YONGSHENG FIBER NEW MATERIAL

Multi-stage targeting bionic nano drug delivery system for nasal delivery and preparation method and application of multi-stage targeting bionic nano drug delivery system

The invention discloses a nasal delivery multistage targeting bionic nano drug delivery system as well as a preparation method and application of the nasal delivery multistage targeting bionic nano drug delivery system. According to the drug delivery system, a black phosphorus nanosheet with an active oxygen scavenging effect is used as an inner core carrier, a neuroprotective agent fingolimod hydrochloride FTY720 and polydimethyldiguanide PolyMet are loaded, and a microcolloid cell membrane and a mitochondrial membrane are modified on the surface of the preparation; the nano drug delivery system is applied to treatment of cerebral arterial thrombosis reperfusion injury, can overcome the blood brain barrier, can quickly enter the brain through nasal administration, realizes efficient delivery of drugs, remarkably reduces the cerebral infarction area, reduces the brain inflammation level, protects nerve cells, greatly relieves cerebral arterial thrombosis reperfusion injury, and has application prospects.
Owner:CHINA PHARM UNIV

Aggregation-induced emission photosensitizer as well as preparation method and application thereof

The invention discloses an aggregation-induced emission photosensitizer as well as a preparation method and application thereof. A D-A-pi-A-D symmetrical structure is adopted, the spatial effect and the conjugation degree are adjusted through a pi bridge, the molar absorption coefficient is increased, and singlet excitons are increased; and furthermore, a pyridine ring with positive charges is used for accelerating electron transfer, the yield of triplet excitons and ROS is improved, compared with a traditional photosensitizer, the ROS efficiency is improved by 2-3 times, the efficient killing capability of the photosensitizer on pathogens can be ensured, and a guarantee is provided for rapid healing of infectious wounds. A pyridine ring with positive charges is introduced into an MTCNPy molecule, and the targeting property of the photosensitizer to pathogens is enhanced by utilizing the electrostatic interaction between the pyridine ring and a bacterial negative charge cell membrane, so that the damage to surrounding healthy tissues is reduced. The preparation process is simple, the cost is low, and the hydrogel has excellent hydrophilicity and biocompatibility so as to be more suitable for the biological environment.
Owner:GANSU MATERNAL & CHILD HEALTH HOSPITAL (GANSU PROVINCIAL CENTRAL HOSPITAL)

Green fresh-keeping method for relieving low-temperature cold damage of picked tropical fruits

The invention provides an edible coating fresh-keeping liquid suitable for low-temperature refrigeration of harvested tropical fruits and a storage and fresh-keeping method. The low-temperature cold storage edible film coating fresh-keeping liquid is soybean oil. When bananas or mangoes are coated with the edible coating liquid for low-temperature refrigeration, the occurrence of fruits under low-temperature refrigeration can be effectively inhibited, and the sensory quality and shelf life of the fruits can be maintained. Bananas or mangoes are subjected to soybean oil coating treatment, so that the relative conductivity of fruit tissues can be effectively reduced, and the integrity of fruit cell membranes is maintained; according to the present invention, the production of H2O2, O2 <-> and MDA can be significantly reduced, the activity of POD can be inhibited, and the activity of PPO, SOD and CAT can be enhanced so as to activate the anti-oxidation defense system of fruits, reduce the oxidative damage caused by cold injury, and enhance the accumulation of osmotic regulation substances; the content of antioxidant carotenoid can be increased, so that the stress resistance of the fruits is improved, and the cold injury of the fruits in the low-temperature storage process is reduced.
Owner:HUAZHONG AGRI UNIV

PH-responsive drug self-delivery nano system as well as preparation method and application thereof

The invention relates to the technical field of medical nano-materials, and discloses a pH-responsive drug self-delivery nano-system and a preparation method and application thereof.The preparation method comprises the steps that 1, CMNPs is prepared, specifically, through an amidation reaction, the CMNPs are prepared from methotrexate and cis-aconitic anhydride; step 2, synthesizing DSPE-PEG-T12: synthesizing the DSPE-PEG-T12 by virtue of a nucleophilic addition reaction; step 3, preparing a T12 peptide modified erythrocyte membrane; and step 4, preparing T12-RBCM (at) CMNPs: modifying the T12 peptide of the targeted tumor cell transferrin receptor on an erythrocyte membrane, and coating the CMNPs to construct the nano-acquisition system T12-RBCM (at) CMNPs. According to the nano system T12-RBCM (at) CMNPs obtained by the scheme, the tumor cell uptake efficiency can be remarkably improved, cell apoptosis is induced, and proliferation is inhibited; the long-acting circulation and tumor targeting capability is realized, the tumor growth can be effectively inhibited, and the biological safety is good.
Owner:STOMATOLOGICAL HOSPITAL OF CHONGQING MEDICAL UNIV

Multi-modal subcellular segmentation method and system

Systems and methods for multi-modal subcellular segmentation using photolysable biomarkers and / or transcriptomic readout density maps are disclosed. The systems and methods improve the accuracy of cell segmentation of the nucleus, cytoplasm, and cell membrane regions by using optical and bleach correction from a variety of photolysable morphological markers in combination with high quality 3D images acquired with high dynamic range scans and spatial transcriptomic readout density maps.
Owner:BRUKER SPACE BIOLOGY

Modified ligand-gated ion channels and methods of use

ActiveUS12570706B2Hydrolysed protein ingredientsNervous disorderCell membraneLigand-gated ion channel
This document relates to materials and methods for controlling ligand gated ion channel (LGIC) activity. For example, modified LGICs including at least one LGIC subunit having a modified ligand binding domain (LBD) and / or a modified ion pore domain (IPD) are provided. Also provided are exogenous LGIC ligands that can bind to and activate the modified LGIC, as well as methods of modulating ion transport across the membrane of a cell of a mammal, methods of modulating the excitability of a cell in a mammal, and methods of treating a mammal having a channelopathy.
Owner:HOWARD HUGHES MEDICAL INST

Optimization system of pancreas islet repair ICK mode oral polypeptide with VDAC1 as target spot based on ESM2 and genetic algorithm

PendingCN121393529AChemical property predictionMolecular designDimerMutation frequency
The invention relates to an islet repair ICK mode oral polypeptide optimization system taking VDAC1 as a target based on ESM2 and a genetic algorithm, and the system comprises a mutation module which is used for carrying out mutation and recombination by adopting the genetic algorithm according to original protein sequence data to generate an initial population; the prediction module is used for respectively inputting the protein sequence data of the initial population into a protein dimer dissociation energy prediction model, a protein toxicity prediction model and a protein cell penetrability prediction model to obtain a dissociation energy prediction value delta G, a toxicity probability value T and a cell membrane passing transmission probability Ed; and the optimization module is used for acquiring the optimal individual performance of the highest fitness score to output the Pareto optimal sequence index, and recording the hotspot residue distribution and the site mutation frequency at the same time. On the basis of keeping the biological activity of the original sequence, the key drug properties are improved in a breakthrough manner.
Owner:HUBEI UNIV OF TECH

7-dehydrocholesterol liposome and application thereof

The invention relates to the technical field of biological medicine, in particular to 7-dehydrocholesterol liposome and application thereof. According to the invention, a biological membrane modified liposome is constructed, and the biological membrane modified liposome comprises an ROS responsive liposome, a neutrophile granulocyte membrane and a renal tubular epithelial cell membrane; the biofilm modified liposome has an active oxygen species (ROS) responsive release function and a dual targeting capability, has the advantages of an integrated drug effect carrier, ROS triggered release, dual bionic targeting, strong target cell uptake and multi-mechanism ferroptosis resistance, and can be used for preparing the biofilm modified liposome. The compound can be used for treating ferroptosis related diseases such as acute kidney injury, myocardial ischemia-reperfusion injury and cerebral ischemia-reperfusion injury, and has wide clinical popularization value.
Owner:THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV

Multifunctional nano-particle targeting abdominal aortic aneurysm and preparation method and application thereof

The application provides a multifunctional nano particle for targeting abdominal aortic aneurysm and a preparation method and application thereof, and belongs to the field of nanobiomedicine, wherein polyphenol oxidation self-polymer nanoparticles are used as carriers, doxycycline is loaded, and a targeting ligand cRGD is modified on the surface of the nano carrier; the neovasculature in the media and adventitia of AAA sites helps accumulation of the nanoparticles in the abdominal aortic aneurysm, and the integrin αν3 beta receptor highly expressed on the surface of the diseased cell membrane can recognize the cRGD with high affinity, and then mediate the targeted endocytosis of the nanoparticles; after intravenous injection, the nanoparticles can be effectively enriched in the lesion site and achieve long-term retention, and can release DC in response to the high ROS level in the tumor microenvironment; the drug is rapidly released to take effect, and the non-specific toxic side effects are reduced; the free radical scavenging capacity of the nanoparticles can be synergized with the DC activity to treat AAA through multiple mechanisms such as anti-inflammatory, antioxidant, macrophage repolarization promotion, anti-apoptosis and calcification inhibition, and MMPs inhibition.
Owner:CENT SOUTH UNIV

Use of 5,8,11-eicosatrienoic acid in the prevention and treatment of plant fungal diseases

PendingCN122139747ABiocideFungicidesBiotechnologyPhytophthora sp.
The application discloses application of 5,8,11-eicosatrienoic acid in prevention and treatment of plant fungal diseases and belongs to the field of pesticide chemistry. The 5,8,11-eicosatrienoic acid mainly prevents and treats diseases caused by the oomycete class capsicum phytophthora, the compound is a long-chain unsaturated fatty acid, has an antifungal disease effect and can be used as a fungicide to prevent and treat plant diseases. The compound can effectively prevent pathogenic bacteria from invading plants by inhibiting mycelium growth, enhancing cell membrane permeability and interfering with the spore sac release process, and is further used for preventing and treating huge plant fungal diseases. Biological tests show that the compound has a significant concentration-dependent inhibitory effect on the mycelium growth of the capsicum phytophthora, and the half effective inhibitory concentration (EC50) is 60.2 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is more than 83%, the 5,8,11-eicosatrienoic acid can effectively inhibit the release of the capsicum phytophthora spore sac, and the EC50 is 156.3 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is morethan 77%.
Owner:HUAZHONG AGRI UNIV

Anti-PD-1 antibody, CAR-T cell, and preparation method and application thereof

The invention provides an anti-PD-1 antibody, a CAR-T cell, and a preparation method and application thereof. The anti-PD-1 antibody comprises LCDR-1-3 as shown in SEQ ID NO: 1-3 and HCDR-1-3 as shown in SEQ ID NO: 4-6, respectively. The CAR-T cell expresses a novel element for efficiently blocking the PD-1, and the element is a single-chain antibody for targeting the PD-1 in a cell membrane anchoring manner. And the chimeric antigen receptor and the cell membrane anchored anti-PD-1 scFv are connected by a 2A cleavage protein. The membrane anchor type anti-PD-1 scFv expressed by the CAR-T cell can almost completely block the expression of PD-1 on the surface of a T cell membrane, and further block a signal channel combined with PD-1 / PD-L1, so that the capability of T cell depletion caused by antagonism tumor of the CAR-T cell is enhanced, and the purpose of enhancing the anti-tumor effect of the CAR-T cell is achieved.
Owner:SHANGHAI YIHAO BIOTECH CO LTD