The present invention discloses a cell nutrition enhancing health product preparation and a preparation method thereof, and relates to the technical field of health care products, the cell nutrition enhancing health product preparation is made from the following materials: wall-broken ganoderma lucidum spore powder, selenium-rich yeast, dendrobium officinale freeze-dried powder, ganoderma lucidum extract, astragalus extract, wolfberry extract, rhizoma polygonati powder, Ganoderma sinensis powder, Antrodia powder, American ginseng powder, lentinan, pine pollen, spirulina powder and royal jelly powder, the cell nutrition enhancing health product preparation can activate the electronic delivery system on mitochondrial inner membrane, increase cell mitochondrial ATP production, fundamentally supplement cellular energy, eliminate cell fatigue, and enhance cell regeneration ability, and is free of side effects, by insisting on taking, the cell nutrition enhancing health product preparation helps to enhance physique and improve health state, and is suitable for the sub-health crowd to take.