67 results about "Cellular energy" patented technology

The invention discloses a gelatin microcarrier lysate as well as a preparation method and application thereof. The gelatin microcarrier lysis buffer comprises lyase, a cell protective agent, a cell energy metabolism protective agent, an antioxidant, a buffer solution and a chelating agent. The gelatin microcarrier lysate has extremely strong protectiveness to cells, abandons damaging trypsin and EDTA, and innovatively adds a cell protection combination of a cell protective agent L-arginine, a cell energy metabolism protective agent sodium pyruvate and an antioxidant vitamin C, so that the cell survival rate (gt; 98%) and the retention rate (gt; the method is high in cell yield, good in safety and wide in range.

The invention relates to a nerve repair guide catheter, a preparation method and application, an electrostatic spinning technology is adopted to construct a nanofiber membrane which is directionally and orderly arranged so as to guide nerve axons to directionally grow, and cell energy metabolism is promoted and tissue repair and regeneration are accelerated through metabolism and conversion of degradation products. Therefore, the nerve injury repairing effect better than that of traditional matrix materials without biological activity, such as PCL, is achieved. According to the invention, a novel solution is provided for solving the problem of insufficient biological activity of a traditional nerve conduit material by constructing a degradable high polymer material with biological activity. Therefore, the regeneration efficiency of peripheral nerve injury repair is improved, and the application prospect of the bioactive material in the fields of tissue engineering and regenerative medicine is expanded.

The invention belongs to the technical field of cosmetics, and particularly relates to a cell energy activation composition with an anti-aging effect and a preparation method thereof, the cell energy activation composition with the anti-aging effect and the preparation method thereof are characterized in that the cell energy activation composition with the anti-aging effect comprises the following components: 3-10 parts of thiotaurine and 0.01-0.5 part of a metal ion compound, the composition is prepared from the following raw materials in parts by weight: 0.05-0.5 part of an antioxidant, 0.005-0.05 part of redox regulatory protein, 0.1-6 parts of a raw grass-containing extract, 0.01-1 part of 4-tert-butyl cyclohexanol and 0.05-3 parts of a lipidosome delivery system, according to the invention, multi-channel energy activation is carried out: thiotaurine participates in energy metabolism, metal ions promote enzyme activity, thioredoxin maintains redox balance, and mitochondrial functions are comprehensively improved; liposomal delivery enhances absorption: the transdermal ability of active matters is improved, the stability is enhanced, and the utilization rate is increased; multiple anti-aging mechanisms are as follows: oxidation resistance, saccharification resistance and cell repair.

This invention relates to the field of cosmetic compositions, and more specifically to a synergistic anti-aging composition involving energy metabolism and autophagy, its preparation method, and its application. The composition comprises sea buckthorn fruit extract, trehalose, and a cosmetically acceptable polyol carrier. This invention pioneers a betaine-trehalose-NADES extraction system, achieving low-temperature, high-efficiency extraction while preserving the heat-sensitive active ingredients of sea buckthorn. Trehalose possesses both extraction and autophagy activation effects. A selective separation strategy achieves a balance between efficacy, compliance, and process simplicity. The green, circular process reduces costs and is environmentally friendly. Through the synergistic effect of sea buckthorn fruit extract and trehalose, the composition enhances mitochondrial membrane potential, increases ATP production, inhibits excessive activation of the mTOR pathway, and promotes LC3-II conversion, thus synergistically combating skin aging from multiple targets including cellular energy metabolism, signaling pathways, and autophagy.

The invention discloses a multi-system function regeneration factor composition as well as a preparation method and application thereof. The multi-system function regeneration factor composition is prepared from the following components in parts by mass: 0.8 to 2.4 parts of melatonin, 84 to 126 parts of nicotinamide ribose and 42 to 84 parts of resveratrol. According to the multi-system functional regeneration factor composition disclosed by the invention, through scientific proportioning and synergistic effect of melatonin, nicotinamide ribose and resveratrol, the functions of stem cells are strongly activated, proliferation of the stem cells is remarkably promoted, the stemness maintaining capability of the stem cells is improved, osteogenesis and adipogenesis differentiation potential is enhanced, and the regeneration effect of the stem cells is improved. Meanwhile, an NAD < + > metabolic pathway is activated so as to strengthen cell energy and anti-aging capability. The capsule adopts the design of combining quick-release pellets and sustained-release pellets, the quick-release part can quickly release melatonin, the local drug concentration can be increased in a short time, and stem cell activation signals can be quickly started; and the sustained release part slowly releases nicotinamide ribose and resveratrol to maintain the long-acting stability of the drug concentration, so that the effect taking speed is ensured, the action time is prolonged, and the bioavailability is greatly improved.

The invention belongs to the technical field of formula foods for special medical purposes, and particularly relates to a fat component for treating Alzheimer's disease and a preparation method of the fat component. The fat component is prepared from the following raw materials in parts by weight: medium-chain triglyceride, starch sugar, silicon dioxide, sodium caseinate, starch sodium octenylsuccinate, mono-glycerol fatty acid ester, diglycerol fatty acid ester and potassium hydroxide. And a neuroprotective nutrient combination. According to the invention, the medium-chain triglyceride capable of supplying energy quickly is creatively combined with the nerve protection nutrient with a specific mechanism. The medium-chain triglyceride provides efficient ketone energy; magnesium citrate stabilizes the excitability of neurons; the nicotinamide ribose supports cell energy metabolism; the omega-3 fatty acid plays roles in resisting inflammation and repairing nerve cell membranes. The four components provide protection from energy supply, neuron stabilization, metabolism support and structure repair, and bring better neurological function prognosis while controlling the Alzheimer's disease.

The invention discloses a method and a device for detecting the energy metabolism level of cells and organoids. According to the detection method disclosed by the invention, the acidification rate and the oxygen consumption rate of the extracellular microenvironment are obtained through signal response of hydrogen ions and the dissolved oxygen sensing electrode, and meanwhile, a dynamic detection microcavity is constructed by utilizing a metabolism detection device, so that the sensitivity and the accuracy of measurement are improved. By adopting different drug intervention schemes, the energy metabolism pathway of the cells can be regulated and controlled, so that the evaluation of the two energy metabolism pathways of glycolysis and aerobic respiration of the cells is realized. The in-situ and high-sensitivity cell energy metabolism detection method and device are provided by detecting the concentration change of the specific analyte of the extracellular microenvironment, the method has the universality of two-dimensional and three-dimensional cell models, a brand new platform is provided for scientific research and drug effect screening based on an in-vitro model, and the application prospect is wide.

The application discloses a method for inducing T cell reprogramming into NK-like cells, comprising the following steps: (1) culturing the T cells in a culture system to obtain activated T cells; (2) adding small molecule inducers twice to the activated T cells to obtain NK-like cells; wherein the culture system in step (1) contains 1-10 v / v % serum substitute and / or platelet lysate, and does not contain fetal bovine serum. By optimizing the culture process and precisely adding drugs twice, the small molecule accumulation toxicity caused by multiple drug additions is avoided, the damage to the structure and function of the mitochondrial membrane is reduced, the mitochondrial membrane potential is significantly improved, the energy metabolism capacity of the mitochondria is enhanced, sufficient energy support is provided for the long-term survival and function maintenance of the cITNK cells, and the physiological activity of the cells is ensured from the energy metabolism level.

The invention relates to the technical field of biology, and discloses application of ascaroside in anti-aging, and the ascaroside can be widely applied to the fields of anti-aging foods, nutritional supplements and medicines. The invention reveals that ascaroside asc-C9 can play a cross-species conservative anti-aging effect from insects to mammals to a fermentation system for the first time, and the mechanism of ascaroside asc-C9 comprises promotion of mitochondrial function, enhancement of fat browning, improvement of antioxidant level and maintenance of cell energy steady state; the invention provides an anti-aging active molecule which is natural in source and clear in effect and a fermentation preparation approach thereof.

The present application provides a kind of fluorine boron compound, the fluorine boron compound contains anthraquinone group, can generate strong oxidizing anion radical after photoexcitation efficiently, thus has lower half inhibitory concentration, stronger inhibitory effect, has stronger phototoxicity, can kill cancer cells in shorter time;Meanwhile, the superoxide anion radical generated can specifically oxidize FADH2 coenzyme in mitochondrial electron transport chain, by oxidizing its isoalloxazine ring structure to cause coenzyme inactivation, then mitochondrial electron transport process, and inhibit cancer cell energy metabolism, so as to effectively inhibit the proliferation activity of cancer cells.

The invention discloses a composition with a tumor mitochondrial dual regulation effect, temperature-sensitive hydrogel and application, and belongs to the technical field of biological medicine, the composition is composed of a peroxide initiator and a mitochondrial autophagy inhibitor, the peroxide initiator is used for causing mitochondrial damage, and the mitochondrial autophagy inhibitor is used for causing mitochondrial autophagy. The mitochondrial autophagy inhibitor is used for inhibiting mitochondrial The peroxide initiator is indocyanine green, chlorin, iron oxide nanoparticles or Fe-MOFs (Metal-Organic Frameworks); the mitochondrial autophagy inhibitor is chloroquine, 3-methyladenosine or bajulomycin A1; the tumor is specifically melanoma; the combination of the peroxide initiator and the mitochondrial autophagy inhibitor can better inhibit the energy metabolism of tumor cells and inhibit the occurrence and development of tumors, and after the composition is delivered through the temperature-sensitive hydrogel carrier, the treatment effect of the composition on tumors is further improved.

1. Name of the product in this design: Physiotherapy Chamber. 2. Purpose of this design: A 360° internal heat-transmitting cell energy chamber for use during physiotherapy. 3. The key design feature of this product is its shape. 4. Images or photos that best illustrate the design key points: Combined state reference diagram.

The invention discloses application of R-1, 3-butanediol in preparation of anti-aging cosmetics or medicines, and relates to the technical field of cosmetics. According to the embodiment of the invention, the R-1, 3-butanediol is applied to the skin, and the R-1, 3-butanediol is explored and proved to be capable of exceeding the traditional physical and chemical functions of R-1, 3-butanediol, being utilized by cells, participating in the energy metabolism process of the skin cells, regulating the energy metabolism of mitochondria and regulating the expression of AMPK protein in the skin cells; the skin function is improved from the fundamental level of cell energy supply, and the skin aging problem caused by cell metabolism decline is resisted. The positioning and action mechanism is different from all known traditional applications, and represents a brand-new application mode of the component in cosmetics based on cytobiology. The functional skin care product is expected to be promoted to be upgraded to the direction of cellular metabolism regulation, and is popularized and applied.

The invention discloses an organic acid-containing anti-hair-loss composition based on a cacumen biotae extract, which comprises the cacumen biotae extract and an organic acid as anti-hair-loss active components, and the acid is succinic acid or malic acid or a combination of the succinic acid and the malic acid. The invention also provides application of the anti-hair loss composition in regulating hair follicle energy metabolism and preventing hair loss. The composition containing the cacumen biotae extract, the succinic acid and the malic acid can promote the activity of hair papilla cells, enhance the energy metabolism of the cells, recover the function of hair follicles damaged by oxidation and recover the energy metabolism of the hair follicles damaged by oxidation, so that the composition has a good effect of preventing and treating alopecia, stimulates hair growth and has few toxic and side effects.

The invention discloses a cell energy cabin with an in-cabin body position fixing structure, and relates to the technical field of cell energy cabins, the cell energy cabin comprises a physiotherapy table assembly and adjustable limiting frames, the adjustable limiting frames are horizontally installed on the middle surfaces of the left side and the right side of the physiotherapy table assembly, and a far infrared component is erected at one end of the physiotherapy table assembly; and the left and right ends of the bottom of the far infrared component are horizontally connected with the structure adjusting components. According to the cell energy cabin with the in-cabin body position fixing structure, through the use of the adjustable limiting frame, flexible adjustment and accurate positioning can be carried out according to the body shape characteristics of different users, it is ensured that the users keep stable and comfortable body positions in the cabin, the situation that the physical therapy effect is affected due to body position changes is effectively avoided, and the physical therapy effect is improved. Through the arrangement of the far infrared component, the cell energy cabin can utilize the far infrared generation plate to generate far infrared rays to irradiate a user in all directions, blood circulation is promoted, cell viability is enhanced, and the physical therapy effect is further improved.

Helmet (1) comprising: at least a cellular energy-absorbing insert (2); a foam liner (3) comprising at least one recess (4) shaped to accommodate the at least one cellular energy-absorbing insert (2); the foam liner (3) also comprises at least one vent (6) for allowing an air transit from outside the helmet (1) to the cellular energy-absorbing insert (2); wherein the at least one vent (6) is partially closed such that the at least one cellular energy-absorbing insert (2) is not visible from outside the helmet (1) through the vent (6).

The present invention relates to a cosmetic composition for improving the skin condition comprising nicotinamide mononucleotide (NMN). Specifically, the present invention provides a composition comprising a sponge microneedle, a lactic acid bacteria exosome, and ceramide in a weight ratio of 0.6: 3.0: 0.01, the lactic acid bacteria exosome being obtained by culturing a Lactobacillus curvatus GFC-AM5 strain (Concentration Number: KCCM12245P) at 37 DEG C for 20 hours and then concentrating the strain by 40 times or more, and the ceramide being a mixture of the sponge microneedle, the lactic acid bacteria exosome, and the ceramide. Wherein the nicotinamide mononucleotide (NMN) is contained in an amount of 0.001 to 3% by weight relative to the total weight of the composition. The sponge microneedle promotes transmission of effective components by forming a microchannel on the skin surface, the exosome provides a cell signal transduction function, the NMN promotes cell energy metabolism by activating an NADbiosynthetic pathway in skin cells, so that cell proliferation and regeneration are enhanced, and the ceramide provides a function of strengthening a skin barrier. Accordingly, the composition of the present invention can simultaneously achieve the effects of improving skin barrier, activating cells and promoting wound healing, and can be applied to various cosmetic formulations.

The invention provides a quick response type nutrient composition for a cognitive load scene. The quick response type nutrient composition is prepared from the following raw materials in parts by weight: 400 to 600 parts of L-alpha-choline alfoscerate, 0.1 to 0.4 part of pyrroloquinoline quinone disodium salt, 4 to 6 parts of blueberry anthocyanin, 4 to 6 parts of gamma-aminobutyric acid, 4 to 6 parts of L-lysine and 4 to 6 parts of L-arginine. The invention further provides a preparation method and application of the quick response type nutrient composition for the cognitive load scene. Through the synergistic effect of the six components, a nerve cell energy metabolism-neurotransmitter balance-oxidation protection three-channel synergistic mechanism is formed, through the multi-target-point synergistic effect, the prepared nutrient composition has the multiple effects of improving the cognitive decline symptom, rapidly relieving cognitive fatigue, improving the thinking agility and reducing the error rate; the method is especially suitable for a high-intensity cognitive load scene. The invention belongs to the technical field of food processing.

The invention discloses a camellia flower composition with waterproof, anti-sweat and photo-thermal multiplication effects as well as a preparation method and application of the camellia flower composition. The camellia flower composition is prepared from a camellia extract, polydimethylsiloxane, a physical sun-screening agent, C12-15 alcohol benzoate, a water-in-oil emulsifier, trimethylsiloxy silicate ester, modified disteardimonium hectorite, silica, a spirulina platensis extract, glucosylrutin, p-hydroxyacetophenone, 1, 2-hexanediol and water. The invention discloses a preparation method of the camellia flower composition and application of the camellia flower composition in preparation of skin care or sunscreen products of cream or emulsion. The camellia flower composition can quickly and uniformly form a film on the surface of skin, improves the absorbance and sunscreen efficiency of a product at a specific wavelength, enhances the waterproof, sweat-proof and heat-proof effects, and also has the effects of changing the wavelength of ultraviolet rays, converting harmful light in the ultraviolet rays into light beneficial to the skin and further converting the light into cell energy, so that the skin-care effect is improved. The effects of promoting collagen generation, relieving and repairing and the like are achieved.

The invention belongs to the field of biomedical functional peptides and healthy foods, and particularly relates to a sea cucumber peptide for repairing gastrointestinal mucosal injury and a preparation method thereof. The sea cucumber peptide is prepared from sea cucumber oligopeptide, 2-hydroxy-4-methoxyphenylacetamide, beta-cyclodextrin, glycerol, potassium sorbate and deionized water, wherein the sea cucumber oligopeptide is modified by a glycyrrhizic acid-amino acid deep eutectic system. In the modification process, a deep eutectic system is formed through monosodium glycyrrhizinate and L-glutamic acid, and the sea cucumber oligopeptide is subjected to hydrogen bond recombination and amidation reaction, so that the structural stability and bioavailability of the sea cucumber oligopeptide in an acid environment are improved from the molecular level; the 2-hydroxy-4-methoxyphenylacetamide is used as a phenylacetamide organic small molecule with a simple structure, and can be used for cooperatively regulating the energy metabolism of cells and promoting the regeneration of epithelial cells of mucous membranes. The preparation method is mild and controllable, and the product is safe and stable, and can be widely applied to the fields of gastrointestinal repair preparations and functional foods.

The invention discloses a slow-release nutrition preparation for small needle knife guide in an ovarian reflex region and a preparation method of the slow-release nutrition preparation, and relates to the technical field of medical nutrition preparations. 20 to 30 parts of a slow release carrier; 3-6 parts of an absorption enhancer; 45 to 65 parts of a biocompatible auxiliary material; 1-3 parts of a stabilizer; according to the invention, the D-chiro-inositol is helpful for adjusting hormone balance in vivo and promoting ovarian health; glutathione is a potent antioxidant and protects ovarian cells from oxidative damage; the Q10 coenzyme can enhance cell energy metabolism and improve the ovarian function; soybean isoflavone is phytoestrogen and can simulate the action of estrogen of a human body, the core nutritional ingredients are combined with a slow release carrier prepared from chitosan, sodium alginate and a medicinal fungal polysaccharide compound, slow release of nutrient substances is achieved, action time is prolonged, bioavailability of the nutrient substances is improved, the substances are combined, and the slow release effect is achieved. The nourishing and long-acting protection on the ovary reflex region are realized.

The invention discloses a traditional Chinese medicine concentrated solution for cell energy activation type scoliosis and a preparation process, and relates to the technical field of traditional Chinese medicine processing. The traditional Chinese medicine concentrated solution is prepared from the following raw materials in parts by weight: 100-140 parts of radix dipsaci, 80-120 parts of cortex eucommiae, 130-170 parts of radix astragali, 60-100 parts of radix angelicae sinensis, 50-70 parts of rhizoma chuanxiong, 60-80 parts of radix angelicae pubescentis, 80-100 parts of fructus lycii, 40-60 parts of radix glycyrrhizae and 15-25 parts of activation auxiliary materials. Himalayan teasel root and eucommia ulmoides in the formula are core components for strengthening muscles and bones and can enhance the toughness of ligaments and tendons around the spine; radix astragali can tonify qi, angelica sinensis can nourish blood, local blood circulation of the spine can be improved, and nutrition is provided for tissue repair; ligusticum wallichii can promote blood circulation to remove meridian obstruction, radix angelicae pubescentis can expel wind-damp, and inflammation and adhesion of soft tissues around The Chinese wolfberry fruits can nourish the liver and the kidney, the licorice roots are matched to harmonize all the medicines, the added activating auxiliary material semen cuscutae extract can activate energy metabolism of osteoblasts and chondrocytes around the spine in a targeted mode, collagen synthesis and calcium deposition are promoted, and tissue damage related to spine malformation is repaired from the cellular level.

Compositions comprising a peppermint concentrate are provided. The compositions may be administered to an individual in need thereof for (i) improving a physiological state associated with metabolic fatigue in one or more cells of the individual, and / or (ii) increasing mitochondrial energy and mitochondrial calcium uptake in one or more cells of the individual, and / or (iii) enhancing mitochondrial function in the individual, and / or (iv) treating or preventing a calcium deficiency / depletion disorder in the individual. Additionally or alternatively, the compositions may be administered to treat or prevent a mitochondrial-related disease or a condition associated with a change in mitochondrial function in an individual in need or at risk.

The invention belongs to the technical field of algae development experiments, and particularly relates to a method for measuring energy metabolism of chlorella mitochondrial stress cells. According to the method, an Agilent cell energy meter XF24 is utilized, the setting of an Agilent default experimental program is optimized, and a mixing part in all reading programs is canceled; the drug concentrations of the three targeting drugs added into the mitochondrial electron transport chain are designed through a large number of research tests, and the number of measurement cycles (5-6 times) after the drugs are added is increased, so that the oxygen consumption rate of cells is measured to obtain key parameters reflecting the mitochondrial function, and the measurement result is more accurate. According to the method, a chlorella mitochondrial pressure curve graph is given, and a new research direction is provided for energy metabolism of algae cells.

The polypeptide composition is prepared from the following components in percentage by weight: 0.1 to 20 percent of carnosine, 0.01 to 10 percent of oligopeptide-1, 0.001 to 5 percent of blue copper peptide, 0.001 to 2 percent of DNA (deoxyribonucleic acid) sodium, 0.0001 to 1 percent of red scorpion four-membered cyclic peptide, 28.989 to 45 percent of betaine and 40 to 48.499 percent of sorbitol. Red scorpion four-membered cyclic peptide cooperates with the cell proliferation promoting function of tripeptide-1 copper and oligopeptide-1, the antioxidant effect of carnosine and the effect of DNA sodium activating mitochondrial metabolism to improve cell energy to achieve the synergistic anti-aging effect, betaine and sorbitol construct an ionic liquid synergistic permeation promoting system, and finally the instant-long-acting integrated anti-aging effect is achieved. The functional mechanism of each component is clear in action site, high in activity, high in acting speed, obvious in curative effect and high in safety, an ionic liquid technology and a penetration enhancing system cooperate to help transdermal absorption of polypeptide components, the active efficacy of the polypeptide components can be more easily exerted, the wrinkle condition of the skin can be improved, and the skin elasticity can be recovered.

The invention provides a tissue preservation solution for autologous tooth transplantation and a preparation method and application thereof, and relates to the technical field of biology, and the tissue preservation solution comprises a high-glucose DMEM culture solution, I TS (insulin-transferrin-selenium), dexamethasone, L-ascorbic acid-2-phosphoric acid and penicillin / streptomycin. The method can provide enough metabolic substrates, improve energy supply of cells, ensure that high activity of periodontal ligament cells is kept within the preservation time of more than 6 hours, and is suitable for long-time transportation and complex clinical operation environments, so that the success rate and clinical effect of autologous tooth transplantation are greatly improved, and the method is suitable for popularization and application. In addition, not only is the cell viability protection capability improved, but also the anti-inflammatory and repairing capabilities are enhanced.

The application discloses a three-layer time sequence regulation type inflammation targeting composite repair system for repairing endometrial injury and a preparation method and application thereof. The system is a three-layer composite structure, which comprises, from outside to inside, (1) an outer ROS response hydrogel layer loaded with anti-inflammatory drugs, which can respond to early high expression of active oxygen to rapidly degrade the drugs, so that acute inflammation is inhibited; (2) a middle layer of targeted delivery and metabolic repair, which is a M1 macrophage membrane pseudo-loaded drug liposome (NMN@M-Lipo), which is exposed after the degradation of the outer hydrogel, actively targets the inflammation lesion through the M1 membrane, and slowly releases nicotinamide mononucleotide (NMN) to restore cell energy metabolism; and (3) an inner layer of cell recruitment and regeneration, which is a decellularized matrix scaffold loaded with SDF-1 alpha, which continuously releases SDF-1 alpha to recruit endogenous stem cells in the later repair stage, and promotes functional regeneration of the endometrium. The application utilizes the time sequence microenvironment changes of endometrial repair, and realizes the cascade regulation of 'anti-inflammation first, metabolic repair second, and regeneration promotion third'. Animal experiments show that the system can significantly restore the reproductive function of the injured endometrium and improve the number of offspring.

This disclosure provides Single cell Isotopomer Distributions and Energetics of Translation under Energetic Stress (SIDETES), a novel strategy to deconvolve metabolic heterogeneity in complex cellular environments, such as tumor and other tumor-resident cell populations, endocrine tissues, whole blood (Peripheral Blood Mononuclear Cells (PBMCs), or lymphoid organs. SIDETES can examine energetic dependence of a substrate, an inhibitor / activator, or a genetic change on rare cells in tumors, and obtain the network response for cells sorted by energy.

The application relates to the field of pet anti-aging and antioxidant medicines, in particular to a pet anti-aging and antioxidant special nutrient supplement and a preparation method thereof. The pet anti-aging and antioxidant special nutrient supplement comprises the following components in parts by weight: NMN 25-35 parts, manganese ascorbate 3-5 parts, coenzyme Q10 30-40 parts, hydrolyzed tannin 5-8 parts, Omega-3 fatty acid 180-220 parts, ergothioneine 2-3 parts, L-carnitine 20-40 parts, betaine 3-5 parts and vitamin E 5-8 parts. The various substances in the formula realize the pet anti-aging and antioxidant functions through a multi-pathway synergistic mechanism, which respectively are direct free radical scavenging, construction of an antioxidant defense network, repair of mitochondrial function, improvement of cell energy metabolism efficiency, blockage of inflammation and metabolic disorder and cutting off of an aging associated pathway.

A composition comprising nettle concentrate is provided. The composition can be administered to an individual in need for (i) improvement of a physiological condition associated with metabolic fatigue in one or more cells, and / or (ii) increased mitochondrial energy and increased mitochondrial calcium uptake in one or more cells, and / or (iii) enhancement of mitochondrial function, and / or (iv) treatment or prevention of calcium deficiency / depletion disorders in the individual. Additionally or alternatively, the composition can be administered for the treatment or prevention of the above in an individual in need of or at risk of treatment or prevention of mitochondrial-related diseases or conditions associated with altered mitochondrial function.