17 results about "Oxidative metabolism" patented technology

The invention discloses a lipid oxidative metabolism characteristic analysis method and system based on REIMS and LC-MS / MS, and belongs to the technical field of lipid oxidative metabolism detection. In order to solve the detection problems of various oxidized lipids, extremely low content in a biological sample and large metabolic fluctuation, the method provided by the invention optimizes a biological sample pretreatment process, sets accurate REIMS and LC-MS / MS technical parameters, adopts two mass spectrometry technologies for combined detection and adopts an internal standard method for quantification, so that the content of the oxidized lipids in the biological sample is greatly reduced, and the content of the oxidized lipids in the biological sample is greatly reduced. And accurate qualitation and quantification of trace lipid oxidation products in a biological sample are realized. The method has the advantages that the trace lipid oxidation products in the biological sample can be accurately qualitative and quantitative, and reliable data support is provided for mining novel biomarkers.

The invention relates to the technical field of biological medicine, and particularly discloses a macrophage membrane coated plateau encephaledema drug carrier and a preparation method and application thereof, and the macrophage membrane coated plateau encephaledema drug carrier is composed of a nanoparticle core and a macrophage membrane shell layer wrapping the core; the nanoparticle core comprises an ROS-responsive carrier material and carbon monoxide release molecules MnCO entrapped in the ROS-responsive carrier material; the content of phosphatidylserine in the shell layer of the macrophage membrane is not less than 5 mol% of the total phospholipid of the membrane. According to the invention, a bionic delivery system of ROS response type MnCO nanoparticles coated with a macrophage membrane is constructed, phosphatidylserine on the surface of the membrane is utilized to actively activate a microglial cell Trem2 receptor, a positive feedback cycle of targeting phagocytosis-CO release-Trem2 up-regulation is formed, and at the same time, fatty acid oxidative metabolism reprogramming is promoted by means of degraded membrane lipid, so that the biomimetic delivery system is used for preparing the ROS response type MnCO nanoparticles. And multi-mechanism synergistic efficient brain-targeted therapy is realized.

Phytol (PHY) is a metabolite of plant chlorophyll, is wide in source and is a chain-like diterpenoid substance consisting of four isoprene units, and the phytol ingested by animals is subjected to oxidative metabolism in vivo, so that not only can energy be provided for the animals, but also the growth of the animals can be promoted, and the growth of the animals can be promoted. Moreover, the phytol and the phytanic acid metabolite of the phytol can also be used as signal molecules to participate in glycolipid metabolism and adipocyte differentiation regulation processes, but people cannot directly take the phytol and the phytanic acid from the nature. The effect of phytol in the generation and development of tumors still has huge unknown property. According to the research, the influence of the phytol and the derivative thereof on the growth of colon cancer, breast cancer and melanoma is detected through an in-vitro experiment CCK-8 (Cell Count Kit 8) and an in-vivo experiment such as a mouse subcutaneous tumor model, and the phytol and the derivative thereof are found to be capable of remarkably inhibiting the growth of colon cancer, breast cancer and melanoma and have no obvious toxic and side effects on heart, liver and kidney functions.

This invention discloses a composition for female ovarian maintenance and its preparation method, relating to the field of health care compositions, including the following steps: mixing pectin and sweetener, dissolving in water to obtain material A; then mixing the traditional Chinese medicine formula, material A, active ingredients, resistant dextrin, vitamins, flavoring agents, and edible flavorings in sequence; stirring after adjusting the volume with purified water, then filtering, filling, sterilizing, and packaging to obtain a composition for female ovarian maintenance; this invention improves the utilization rate of functional components through the immobilization of fructooligosaccharides and β-glucosidase and the enteric coating targeting of shellac; Poria cocos polysaccharide-sulforaphane-resveratrol covers immune, antioxidant, and metabolic regulation pathways, directly targeting the core mechanism of premature ovarian failure; in summary, through the optimization of the entire chain of delivery-conversion-absorption-metabolism, the efficacy is significantly improved under the premise of safety.

This invention proposes a bioecological process for utilizing waste from sugary drinks by producing highly nutritious proteins from single-celled organisms, as well as other molecules of various interest, while significantly reducing the pollutant load of this waste. The process involves the upstream separation of the returned products, which are highly concentrated in sugars. The first step involves preparing the musts for fermentation by degassing and adjusting the sugars to 20 g / L and the pH to 5. An enrichment matrix is ​​used for musts made from carbonated beverage waste. The culture is carried out in batch or semi-continuous mode at a temperature of 30 ± 2°C with an agitation speed of approximately 150 rpm and oxygenation to promote oxidative metabolism. The incorporation of nectars and juices into the fermentation musts of carbonated beverages at a rate of ≥ 50% ensures a significant improvement in biomass yields. The process guarantees almost complete depletion of the sugars initially present in the musts (≥ 95%), producing biomass with a protein content exceeding 50% on a dry matter basis, which can be used in animal feed, aquaculture, or cosmetics.

This method and composition involves oral administration of a low dose of D-ribose to enhance adenosine monophosphate-activated protein kinase (AMPK) activity in both exercising and sedentary mammals, improve mitochondrial-related oxidative metabolic parameters, and thereby promote overall metabolic health in mammals.

The invention relates to application of a lotus plumule extract in preparation of drugs for activating Rab5a / PPARalpha signal axis, promoting fatty acid oxidation and reducing myocardial lipid toxicity injury. Animal experiments verify that the plumula nelumbinis extract effectively inhibits abnormal weight growth, reduces fat deposition, reconstructs fatty acid oxidative metabolism steady state, improves myocardial lipid toxicity injury and maintains cardiac functions by activating Rab5a / PPARalpha signal axis, and the plumula nelumbinis extract is stable in extraction process, clear in mechanism, small in toxic and side effects and easy to popularize. The problem that existing lipid metabolism abnormity intervention medicine targets and components are single can be solved, and a new scheme is provided for medicine research and development of metabolic diseases.

This disclosure describes the characteristics of the “energetic” cancer stem cell (e-CSC) phenotype. This distinct sub-population of cancer stem cells (CSCs) has a unique energetic profile compared to bulk CSCs, being more glycolitic, having higher mitochondrial mass and elevated oxidative metabolism. e-CSCs also show an increased capacity to undergo cell cycle progression, enhanced anchorage-independent growth, and ALDH-positivity. The e-CSC phenotype presents new targets for cancer therapeutics, and in particular the anti-oxidant response, mitochondrial energy production, and mitochondrial biogenesis of e-CSCs makes them highly susceptible to mitochondrial inhibitors that target e-CSC anti-oxidant response, mitochondrial energy production, and mitochondrial biogenesis. Gene products for e-CSCs are disclosed, as well as classes of mitochondrial inhibiting therapeutic agents. Also disclosed are methods for identifying and separating e-CSCs front bulk cell populations.

The invention discloses a medicine for inhibiting gastric cancer cell proliferation based on fatty acid metabolism regulation, and a composition, a preparation method and application thereof, and belongs to the technical field of anti-tumor medicines and metabolism regulation medicines. The drug comprises an FA2H catalytic product which is (R)-2-hydroxy fatty acid or a pharmaceutically acceptable salt thereof; the composition comprises an FA2H catalytic product, an SCD1 inhibitor and an FASN inhibitor, and by synergistically inhibiting fatty acid uptake, synthesis and oxidative metabolism, the proliferation rate and migration ability of gastric cancer cells are remarkably reduced. The core problems that in the prior art, single target inhibition is prone to escape, chemotherapy drug resistance is high, and clinical transformation is difficult are solved.

To provide compounds to increase muscle mass, muscle oxidative capacity, and muscle function and to treat muscular dystrophy, in particular limb girdle muscular dystrophy type 2A / R1 / D1, compositions including the compounds, and treatment methods.SOLUTION: The present invention provides a compound having the chemical structure of formula (I), and a composition comprising the compound or a pharmaceutically acceptable salt or solvate thereof.SELECTED DRAWING: None

This invention relates to the field of cell biology, and discloses a culture medium and method for inducing physiological myocardial hypertrophy based on carnosine. The culture medium for inducing physiological myocardial hypertrophy contains carnosine. By introducing an appropriate concentration of carnosine into a cardiomyocyte system, this invention can induce a series of phenotypic changes in cardiomyocytes related to physiological myocardial hypertrophy, including increased cardiomyocyte area, increased mitochondrial number, enhanced oxidative metabolic capacity, and increased ATP production capacity. These changes can improve the energy metabolism level of cardiomyocytes, thus providing a new technical solution for studying myocardial adaptive remodeling.

The invention discloses an essential oil pellet for resisting stress of cats as well as a preparation method and application of the essential oil pellet, and belongs to the technical field of veterinary traditional Chinese medicine preparations. The essential oil pellet is prepared from the following raw materials: extracts of wormwood, lavender and rhizoma acori graminei; the mass ratio of wormwood to lavender to rhizoma acori graminei is (1-2): (1-2): (1-2). The essential oil pellet prepared by the invention is filled into a nylon bag to prepare a sachet, and the sachet is suspended on the neck of a cat, added into a cat collar or put into cat clothes for use, so that the anti-stress capability of the cat can be remarkably improved, specifically, the feed intake of the cat is improved, and the weight of the cat is increased; meanwhile, the antioxidant index is improved, and the level of oxidative metabolite MDA in blood is reduced; in addition, the levels of cortisol and aldosterone as stress hormones and interleukin-6 as an inflammatory cytokine in cat serum can also be reduced.

This invention relates to a selenopeptide that activates TNFR2 and its applications. The TNFR2-activating selenopeptide comprises a tmTNF-targeting peptide, an oligoethylene glycol hydrophilic chain, a selenoamino acid linker, and an alkyl hydrophobic chain connected in sequence. This invention develops a self-assembled selenopeptide with TNFR2-activating activity, which possesses functions of inflammation-targeted delivery, oxidative response phase transition, and reactive oxygen species (ROS) scavenging. In use, it can be pre-assembled into nanomicelles and delivered intravenously to rheumatoid arthritis (RA) tissues for enrichment. In the microenvironment of highly reactive oxygen species in inflamed tissues, the selenoamino acid linker specifically cleaves in response to the overexpression of ROS in damaged cells. This eliminates ROS and reduces oxidative stress. Furthermore, its oxidative metabolite, the tmTNF-targeting peptide, induces liquid-liquid phase separation of tmTNF on the surface of dendritic cell membranes, inducing oligomerization and efficient activation of TNFR2 on the surface of Treg cell membranes, thereby promoting Treg cell proliferation and improving RA inflammation.

The invention relates to the technical field of functional food, and discloses a composite solid beverage for weight management and a preparation method thereof.The core functional component of the composite solid beverage is chia seeds which are used for providing soluble dietary fibers and fatty acid alpha-linolenic acid essential to the human body and swell when encountering water to enhance satiety; the yeast protein is high-bioavailability protein, maintains muscle mass and improves food heat effect; the diglyceride powder is used for replacing common grease and promoting fat oxidative metabolism; the psyllium seed husk powder is high-water-binding-capacity fiber and is used for regulating intestinal tract movement and glycolipid absorption; the synergistic auxiliary material is a prebiotic combination of fructo-oligosaccharide and inulin, bifidobacterium is proliferated in a targeted manner, and intestinal microecology and energy metabolism are improved; the natural fruit powder is used for providing flavor and plant nutrients; the food can be used for preparing non-drug weight management food, and is especially suitable for obese people and metabolic syndrome people who need to control calorie intake for a long time.

The invention discloses a hepatocellular carcinoma prognosis evaluation marker TKFC and application thereof, and belongs to the technical field of prognosis markers. By comparing tumor tissues of patients with different prognosis, the invention discovers that the HCC subtype with relatively strong invasiveness shows a systematic state imbalance, that is, the oxidative metabolism function represented by mitochondrial oxidative phosphorylation and tricarboxylic acid cycle is weakened, and the intracellular proliferation procedure related to chromatin remodeling and cell cycle progress is abnormally active. The state of low oxidative metabolism-high proliferation output may form the metabolism and epigenetic basis of malignant HCC. The low expression of the TKFC is obviously related to high-risk clinical pathological characteristics such as later tumor staging and higher occurrence rate of vascular invasion, and is an independent poor prognosis factor influencing the total lifetime of a patient, which indicates that the TKFC can be used as a prognosis biomarker of HCC.

The invention belongs to the technical field of medical detection, and firstly provides a kit for screening high-risk people with liver injury caused by chemotherapeutic drug methotrexate, and a detection reagent for detecting linoleic acid and oxidative metabolite content of human body is arranged in the kit. The invention also provides an application of the detection reagent in preparation of a methotrexate liver injury prediction product, the detection reagent is a reagent for detecting the content of linoleic acid and oxidative metabolites of the linoleic acid of a human body, and the product is a detection kit. In the invention, when the content of the linoleic acid and the oxidative metabolite thereof is relatively high, high liver injury caused after the methotrexate is used by the human body is predicted. According to the method, high-risk people suffering from methotrexate liver injury can be quickly screened, and intervention can be performed in the early stage so as to reduce the risk of suffering from methotrexate liver injury.

The invention discloses application of TKFC in preparation of a medicine for treating liver cancer, and belongs to the technical field of biological medicine. Experiments prove that the overexpressed TKFC can enhance the oxidative metabolism capability of cells, effectively inhibit proliferation, migration and invasion of the cells, induce ferroptosis and improve the sensitivity of hepatoma cells to ferroptosis inducers, thereby playing a role in treating hepatoma. Meanwhile, through molecular docking, molecular dynamics simulation and cell thermal stability experiments, it is found that the TKFC can be combined with icaritin (ICA), and through TKFC overexpression, the ICA-induced ferroptosis and the anti-tumor effect can be remarkably enhanced. The invention provides a novel method for treating liver cancer.