575 results about "Analytic chemistry" patented technology

The invention discloses an unknown pollutant non-target identification method and system based on mass spectrum and a generative model, and is applied to the technical field of environmental monitoring and analytical chemistry. The method comprises the following steps: collecting mass spectrum data of a water body sample to be detected; preprocessing the original mass spectrum data, and outputting standardized features; inputting the standardized features into a pre-trained generative model to generate a plurality of candidate molecular formulas; using chemical and physical constraints to eliminate candidate molecular formulas which do not conform to rules; executing a rule-driven algorithm to obtain candidate pollutant molecular structures; carrying out comprehensive scoring and sorting on candidate pollutant molecular structures through chemical prior and environmental prior; and semi-quantitative or relatively quantitative concentration determination is carried out. According to the method, a rule-driven expert system and a data-driven generation model are combined, unknown pollutants which do not exist in a standard library can be effectively recognized and analyzed, and full-process automatic processing from original mass spectrum data to pollutant structures and concentrations is achieved.

The invention discloses a metabonomics data batch correction method based on multi-kernel learning, and belongs to the cross technical field of bioinformatics and analytical chemistry. According to the method, the multi-kernel learning technology is utilized, the advantages of different kernel functions are fused in a self-adaptive mode, a model conforming to data reality is constructed, complex drift characteristics of metabolite signals are accurately captured, and efficient and accurate normalization processing of metabonomics data is achieved. Compared with traditional data standardization methods such as SVR and LOESS, the method has the advantages that the performance is excellent in the aspect of reducing the metabolite peak intensity variability, and the data stability is remarkably improved. In the subsequent multivariate statistical analysis, the classification accuracy is greatly improved, the comparability among different batches of data is also remarkably enhanced, reliable data support can be provided for discovery of disease biomarkers, and the method plays a key role in large-scale metabonomics research.

The invention relates to the field of pharmaceutical analytical chemistry, in particular to a method for determining tandospirone and salt intermediates thereof, high performance liquid chromatography is adopted, and chromatographic conditions include that a chromatographic column with octadecylsilane chemically bonded silica as a filler is adopted, and gradient elution is performed by a mobile phase A and a mobile phase B; wherein the mobile phase A consists of a first water phase and acetonitrile; the mobile phase B is composed of a second water phase and acetonitrile; the first water phase is a water solution containing monopotassium phosphate and sodium hexanesulfonate, and the pH value of the first water phase is 3.0-3.8; the second water phase is an aqueous solution containing monopotassium phosphate and sodium hexanesulfonate, and the pH value of the second water phase is 1.8-2.6. The testing method disclosed by the invention has good specificity, accuracy and repeatability, and can be used for monitoring the quality of the tandospirone raw material medicine.

The invention discloses a pesticide residue quantitative analysis system based on Raman spectrum, and relates to the technical field of analytical chemistry and detection.The pesticide residue quantitative analysis system comprises an acquisition module, a processing module, an analysis module and a regulation and control module, the acquisition module is used for acquiring samples and Raman scattering signals, converting the Raman scattering signals into original spectrum data and transmitting the original spectrum data to the processing module; calibrating the original spectrum data, acquiring calibrated spectrum data, transmitting the calibrated spectrum data to an analysis module, comparing the calibrated spectrum data with a pesticide standard spectrum library, outputting a quantitative analysis result of pesticide residues, transmitting the quantitative analysis result to a regulation and control module, storing the pesticide standard spectrum library and the calibrated spectrum data, and correcting sample detection deviation by using the calibrated spectrum data. And through the regulation and control unit, full-process automatic operation from sample processing to result output is realized, the detection reliability is improved through spectrum compensation and characteristic peak comparison, efficient automation is realized through task scheduling and time sequence synchronization, and hardware fault tolerance, authority management and multi-scene adaptation capabilities are realized.

The invention belongs to the field of analytical chemistry, relates to material preparation, and particularly discloses a preparation method of a copper-doped covalent organic framework and application of the copper-doped covalent organic framework in adrenaline colorimetric detection. Firstly, 1H-indazole-4, 7-diamine (Iz) and 1, 3, 5-triformyl phloroglucinol (Tp) are used as monomers to synthesize a novel covalent organic framework (Iz-Tp COF) through a Schiff base reaction, copper ions are introduced into the Iz-Tp COF framework to prepare a novel metal covalent organic framework (Iz-Tp (at) Cu), and then according to the phenomenon that the Iz-Tp (at) Cu can catalyze oxidation color development of epinephrine, the metal covalent organic framework (Iz-Tp (at) Cu can catalyze oxidation color development of epinephrine. According to the present invention, the simple, sensitive and efficient adrenaline colorimetric detection method is established, the test conditions are finally optimized, the standard addition method is combined to accurately determine the adrenaline concentration in the urine, and the good practical application value is provided.

The invention discloses construction and application of a confinement enhanced photoelectrochemistry-electrochemiluminescence dual-mode biosensor, and relates to the technical field of biosensing and analytical chemistry. The preparation method of the biosensor comprises the following steps: taking a CdS (at) SiO2 (at) NaYF4: Yb / Tm nano composite material as a substrate to obtain a confinement enhanced photoelectrochemistry (PEC) and electrochemiluminescence (ECL) sensing interface; in combination with a DNA wheel nanostructure triggered by target acetamiprid, signal amplification is realized; and synchronous dual-mode quenching detection of PEC and ECL signals is realized by virtue of peroxidase-like catalytic precipitation and wide-spectrum absorption characteristics of the PtPd-CoSnO3 nanocube. The biosensor prepared by the invention has the advantages of high sensitivity, strong reliability, good selectivity and the like, can be applied to accurate detection of acetamiprid in samples such as agricultural products and the like, and has important application value in the aspects of environmental monitoring and food safety control.

The invention belongs to the technical field of analytical chemistry and the field of early screening of Alzheimer's disease, and particularly relates to an array sensor based on zirconium-based MOF nano-enzyme and application of the array sensor to detection of amyloid protein. The zirconium-based MOF nano-enzyme for detecting amyloid protein comprises UiO-66 (at) Pt, UiO-66 (at) Pd and UiO-66 (at) PtPd, and the array sensor based on the zirconium-based MOF nano-enzyme is constructed by sensing units which are respectively added with the UiO-66 (at) Pt, the UiO-66 (at) Pd and the UiO-66 (at) PtPd. According to the amyloid protein detection method based on the zirconium-based MOF nano-enzyme, rapid and sensitive detection of amyloid protein can be realized, the cost is low, the repeatability is good, and a new idea is provided for detection of amyloid protein related diseases.

The invention provides a method for detecting vitamin B6 related substances in a multivitamin preparation, and belongs to the field of analytical chemistry. According to the method, a high performance liquid chromatograph is adopted, and chromatographic conditions are as follows: an octadecyl silane bonded silica gel chromatographic column is adopted, a mobile phase is a phosphate buffered solution (containing sodium hexanesulfonate)-acetonitrile, and a mobile phase A and a mobile phase B are performed according to a gradient procedure; the flow velocity is 0.8 to 1.2 mL / min; the column temperature is 28-40 DEG C, the detection wavelength is 320-340nm, and the sample size is 20-50mu L. The detection method has the advantages of high sensitivity and good linear relationship, and is suitable for analysis and content determination of vitamin B6 related substances in various vitamin preparations.

The invention relates to the technical field of environmental analytical chemistry, and discloses a thymol phenolphthalein-TBPE double indicator-based cyanide titration detection method and system, and the method comprises the following steps: adjusting a water sample to be detected to an alkaline pH interval to construct a detection matrix; the method comprises the following steps: introducing a double-indicator system composed of thymol phenolphthalein and tetrabromo phenolphthalein ethyl ester according to a specific ratio into a detection matrix, and constructing a spectrum background for masking yellow interference of sulfide by utilizing the blue color development characteristic of thymol phenolphthalein; dropwise adding a silver nitrate standard solution into the mixed system to execute a complex reaction; according to the method, a spectrum background shielding field is constructed, chromaticity response of a system in a specific wave band is monitored in real time, and when a chromaticity mutation signal from green to bluish violet is captured, a titration end point is judged, and the cyanide concentration is calculated. High-precision anti-interference detection on cyanide in a complex high-turbidity matrix is realized, and the sensitivity and accuracy of end point recognition are improved.

The invention discloses a high-throughput electrochemical detection platform based on an ultra-infiltration micro-droplet array, a preparation method of the high-throughput electrochemical detection platform and application of the high-throughput electrochemical detection platform in AD marker detection, and belongs to the technical field of intersection of analytical chemistry, biosensing and material science. The method comprises the following steps: cleaning and drying a substrate, and immersing the surface of the substrate in a fluorosilane solution to form a super-hydrophobic layer by adopting a dip-coating method; after covering with a mask plate, etching with O2 plasma so as to form a hydrophilic microdot matrix; respectively printing an Ag / AgCl reference electrode, a gold working electrode and a carbon counter electrode on the hydrophilic microdots; electrode wires are printed layer by layer, a reference electrode wire, a working electrode wire and a counter electrode wire are sequentially printed in the area outside the hydrophilic microdot matrix from bottom to top in a spin-coating PDMS layer separation mode after each layer of electrode wire is printed, and PDMS is spin-coated above the counter electrode wire for packaging after printing is completed; and on each gold working electrode, respectively modifying capture antibodies of A beta 42, A beta 40, P-tau181 and P-tau217 proteins through a sample application instrument, so as to obtain the detection kit.

The invention discloses a chain dienol carboxylate compound and a preparation method thereof, alkynyl allyl ester is used as a substrate, in the presence of a nucleophilic catalyst, alkynyl allyl ester, a carboxylic acid compound and an active methylene compound are subjected to a biaddition reaction, and a product containing a chain dienol carboxylate structure is obtained with high double bond cis-trans selectivity. The compound with the chain diene carboxylic ester structure is a chain compound with alternating single and double bonds, and has obvious and important application value in the fields of medicinal chemistry, analytical chemistry, synthetic chemistry and the like. The compound containing the chain-like dialkenyl carboxylic ester structure is a dienol ester compound, and has extremely wide and important application value in many fields such as choline active drug development, fluorescence detection and diagnosis molecular research, natural product synthesis and the like; according to the invention, an efficient synthesis means and a series of candidate compounds for further development can be provided for the development of diene alcohol ester functional molecules with high similarity.

The invention relates to the technical field of analytical chemistry and food pharmacy detection, in particular to a rutin antioxidant activity colorimetric determination method based on a composite nano enzyme, which comprises the following steps: mixing a to-be-detected sample solution with an ABTS free radical cation working solution to carry out first reaction, so that an antioxidant substance in a sample and ABTS free radical cations are subjected to scavenging reaction; adding the composite nano enzyme, a 3, 3 ', 5, 5'-tetramethyl benzidine solution and a hydrogen peroxide solution into a system after the first reaction is completed, and initiating a second reaction; and measuring the absorbance value of the system after the second reaction under a specific wavelength, comparing the absorbance value with the standard curve obtained through the treatment in the same step, and calculating the antioxidant activity expressed by the equivalent concentration of rutin in the sample to be detected. By optimizing reaction conditions, the method provided by the invention simplifies operation steps and can provide accurate measurement results in a wider concentration range.

The invention relates to the technical field of analytical chemistry, in particular to a high performance liquid chromatography detection method of a ridecevir intermediate GS-441524, which comprises the following steps: preparing a sample: taking a GS-441524 test sample, dissolving and diluting with a mixed solution composed of dimethyl sulfoxide and a phosphoric acid solution to prepare a test sample solution; the volume fraction of phosphoric acid in the phosphoric acid solution is 0.05-0.1%, and the volume ratio of dimethyl sulfoxide to the phosphoric acid solution is (30: 70)-(60: 40). According to the application, the ridecevir intermediate GS-441524 is dissolved and diluted through the mixed solution composed of the dimethyl sulfoxide and the phosphoric acid solution, and due to the high solubility of the dimethyl sulfoxide to the ridecevir intermediate GS-441524 and the high boiling point of the dimethyl sulfoxide, the ridecevir intermediate GS-441524 with relatively high concentration can also be subjected to high performance liquid chromatography.

The invention relates to the technical field of analytical chemistry, in particular to an ion chromatography method for separation and detection of acetate, nitrite and nitrate ions in a complex matrix culture medium. The method mainly solves the problems of insufficient separation degree, serious matrix interference, poor detection precision and reproducibility and the like when the conventional ion chromatography technology is used for detecting a complex matrix culture medium.

The present application relates to the field of analytical chemistry, in particular to a method for detecting ten tobacco alkaloids in soil, which uses sodium hydroxide solution and acetonitrile to extract alkaloids in a soil sample, and uses ultra-high performance liquid chromatography-triple quadrupole mass spectrometry to analyze and detect nicotine, nornicotine, myosmine, pseudotropine, anatabine, anabasine, 2,3'-bipyridine, noranatabine, N-methyl pseudotropine and nornicotine, and uses an internal standard method for quantitative analysis. The present application uses sodium hydroxide solution as an alkaloid eluent and acetonitrile as an extractant, which reduces the amount of solvent used, optimizes the operation process, uses sodium chloride to separate the organic phase and the aqueous phase, improves the sample pretreatment efficiency, uses ultra-high performance liquid chromatography-triple quadrupole mass spectrometry to target analysis of 10 tobacco alkaloids, greatly improves the detection sensitivity, and has strong quantitative ability in targeted analysis.

The application relates to the technical field of analytical chemistry detection, and specifically discloses a rare earth ion chromatographic online analysis and detection method and system, which adopts a chromatography-mass spectrometry combined system, realizes multi-scale time-frequency decomposition of signals by constructing an adaptive wavelet base function library, accurately identifies and classifies interference types in combination with a convolutional neural network and vacuum degree coupling analysis; a U-Net generator with an attention mechanism and a multi-scale discriminator are designed for signal repair aiming at repairable interference; a state transition model containing a mass-to-charge ratio database is established, empirical mode decomposition and adaptive filtering technology are used to realize dynamic calibration of a mass axis; quantitative accuracy is ensured through double internal standard correction and a triple verification mechanism; when unrepairable interference is detected, a hierarchical self-checking program is started, and fault diagnosis is carried out in combination with spectrum fingerprint analysis.

The invention discloses an amide chain-bridged bis-tetraphenylethylene fluorescent probe and a synthesis method and application thereof, belonging to the technical field of organic synthesis and analytical chemistry. The fluorescent probe has a structure of an oxalamide-bridged bis-tetraphenylethylene unit and is chemically named 1,2-bis(2-(4-(1-phenyl-2,2-di-dodecylphenyl)vinyl)phenoxyacetyl)ethylenediamine, i.e., Bis-TPE. In the invention, 1,1-di-p-dodecyloxyphenyl-2-phenyl-2-p-hydroxyphenylethylene, 1,2-bis-chloroacetylethylenediamine, and anhydrous potassium carbonate are selected as synthetic raw materials, placed in a dry acetone system for reflux, and after sufficient reaction, the reaction mixture is extracted and distilled under reduced pressure, and the product is separated by silica gel column chromatography, thereby synthesizing the fluorescent probe. The fluorescence intensity of the fluorescent probe of the invention is 1000 nm in Hg 2+ The quenching is obvious in the presence of , and the presence of other ions has little interference with the detection results. The rapid weakening of the fluorescence intensity of the fluorescent probe can be used to detect Hg in the solution. 2+ Highly efficient and selective detection.

The invention discloses a method for separating and determining a dotenorad key intermediate and related compounds thereof by HPLC (High Performance Liquid Chromatography), which belongs to the field of analytical chemistry, and is used for separating and detecting the dotenorad key intermediate (3, 5-dichloro-4-methoxyphenyl) (1, 1-benzo [d] thiazole-3 (2H)-yl) ketone and related compounds A-F thereof by liquid chromatography. A chromatographic column with phenylsilane bonded silica gel as a filler is adopted, a phosphoric acid aqueous solution is used as a mobile phase A, acetonitrile is used as a mobile phase B, gradient elution is carried out, detection and analysis are carried out, and the content of each compound is calculated. According to the present invention, the dotenorad key intermediate and the related impurity compounds thereof can be effectively separated, and the method has advantages of high sensitivity, high separation degree, good repeatability, good durability, simple operation and stable and reliable result, and has important significance on the realization of the dotenorad drug quality control.

The invention discloses a method for determining the content of rubber in a rubber-containing plant based on gel permeation chromatography, and relates to the technical field of analytical chemistry, and the method specifically comprises the following steps: S1, sample pretreatment: drying a rubber-containing plant sample and grinding into powder; s2, rubber extraction: performing ultrasonic extraction on the powder by using a mobile phase of gel permeation chromatography as an extraction solvent, centrifuging, and collecting supernate to obtain an extracting solution; s3, GPC (Gel Permeation Chromatography) detection: filtering the extracting solution, and injecting the filtered extracting solution into a gel permeation chromatographic instrument with a single chromatographic column for detection. The complete plant rubber content determination method is constructed by combining a total rejection GPC principle with an efficient sample pretreatment process, the defects of tedious pretreatment, low detection efficiency or inaccurate detection in the prior art are overcome, the method has the advantages of rapidness, accuracy and the like, extraction and detection parameters can be flexibly adjusted according to different plant samples, and the method is suitable for large-scale popularization and application. The method is suitable for quality analysis and breeding research of various gelatin-containing plants, so that the compatibility is improved.

The invention relates to a preparation method of a molecularly imprinted composite filler, and relates to the technical field of analytical chemistry and environmental monitoring technologies, the preparation method of the molecularly imprinted composite filler comprises the following steps: firstly dissolving template molecules and a first monomer, then adding a cross-linking agent and an initiator, carrying out deoxidation and water bath, and then centrifuging to obtain a template molecule solution; and eluting the precipitate with a flushing fluid, and drying to obtain the microspheres. And dissolving the ionic liquid, uniformly stirring with the obtained microspheres, and carrying out centrifugal separation, washing and vacuum drying to finally prepare the molecularly imprinted composite filler. Finally, the hydrophobic property of the molecularly imprinted composite filler is improved through modification of the ionic liquid and the second monomer. The method provided by the invention has excellent hydrophobic effect and enrichment capability on a target object.

The invention discloses a fluorescence sensing system based on nitrogen-doped carbon dots and machine learning and application of the fluorescence sensing system in recognition of benzenediol isomers, and belongs to the technical field of analytical chemistry and artificial intelligence. According to the invention, the undoped carbon dots and the nitrogen-doped carbon dots of which the properties change in a gradient manner are utilized to form the probe library, the carbon dots in the probe library can perform collaborative recognition, and weak differences between analytes are accumulated and amplified. Although the response difference of each single probe in a probe library to two similar isomers is very small, the tiny differences can be accumulated and amplified in a high-dimensional response vector due to the gradient change of the properties of the probes; the microscopic chemical property difference, which is difficult to directly measure, between the isomers is converted into the macroscopic high-dimensional data mode difference which can be easily identified by a machine, so that the accurate identification and classification of the structural similar substances and the mixture thereof are realized.

The invention discloses an in-situ ultraviolet and visible spectrophotometer with high measurement precision, and relates to the technical field of ultraviolet and visible spectrophotometers, the in-situ ultraviolet and visible spectrophotometer comprises a bottom shell, an optical unit is arranged on the inner wall of the bottom of the bottom shell, and a light source module and a monochromator are arranged in the optical unit; the top of the optical unit is fixedly connected with an operation platform through a supporting rod, the top of the operation platform is provided with a motor mounting frame, the top of the motor mounting frame is provided with a linear motor, the output end of the linear motor is connected with a lifting fixing plate, and the lifting fixing plate is of a Z-shaped structure. The end, away from the linear motor, of the lifting fixing plate is connected with a light shield, and the top of the light shield is provided with an optical fiber clamping frame. According to the method, result deviation caused by dilution multiple estimation errors in a traditional method is avoided, the dilution frequency of the high-concentration sample is reduced, reagent consumption and waste liquid generation are reduced, and the method conforms to the green analytical chemistry concept.

The application relates to a method for enhancing electrochemiluminescence signals by ion-pi interaction, belonging to the fields of electrochemiluminescence and analytical chemistry. 2+ By modifying the working electrode with a conjugated pi system, cation-pi interaction is introduced into the Ru(bpy)3 / triethylamine (TEA) system, efficient enrichment of TEA protonated cations near the electrode is realized, thereby greatly enhancing the electrochemical reaction rate and ECL signal. Further, anion-pi interaction is utilized to realize ECL signal enhancement of the luminol ECL system. In addition, the conjugated pi system is exposed to the outermost boundary of the electrode, so as to provide effective ion-pi interaction for ECL; when the pi system is covered by an alkyl chain, the modified electrode can only exhibit hydrophobic interaction. The method disclosed by the application provides a new strategy for extending ion-pi interaction to all types of ECL systems and constructing a high-sensitivity ECL analysis platform.

The invention belongs to the field of analytical chemistry, and particularly discloses a method for determining trace precious metals in copper-nickel flotation tailings. According to the method, trace precious metal in the tailings is enriched through a nickel matte assaying method, and nickel matte alloy rich in precious metal is formed; the nickel matte alloy is inlaid or crushed and doped with carbon to prepare a sample meeting the measurement requirement of the glow discharge mass spectrometry, and the content of the noble metal in the sample is measured and converted by adopting the glow discharge mass spectrometry. The method effectively solves the problems of low precious metal content in tailings and large fluctuation of detection results, improves the detection accuracy and stability, and is suitable for determination of trace precious metals such as gold, silver, platinum, palladium, rhodium and iridium.

This application relates to the field of analytical chemistry detection technology, and in particular to a sample rack for a liquid chromatography-mass spectrometry (LC-MS) instrument used for TDM detection. The rack includes a support mechanism with a sample placement box at the top and a heat-insulating mechanism at the top. The invention utilizes components such as a pull-out plate, a spare support plate, and a table. The sliding engagement between the pull-out plate and the table allows for convenient horizontal sliding of the pull-out plate to retrieve and place samples placed in a first positioning slot. Simultaneously, the spare support plate is fixedly connected to a support side plate, enabling the categorized storage of samples after testing. These components work together to form an orderly sample management system, achieving the modular, partitioned design that efficiently distinguishes and quickly retrieves samples in different testing states, significantly improving the convenience and standardization of experimental operations.

The invention relates to the technical field of analytical chemistry detection, in particular to a method for determining trace nitrite in pharmaceutic adjuvants.The method comprises the steps that an acetonitrile solution, a potassium hydroxide solution, a phosphate buffer solution, a sulfuric acid solution, a 2, 3-diaminonaphthalene solution and a reference substance working solution are provided; the method comprises the following steps: preparing a standard curve solution and a test sample solution, and carrying out ultra-high performance liquid chromatography-tandem mass spectrometry analysis detection: respectively carrying out sample injection detection on the test sample solution and the prepared standard curve solutions with different concentrations under preset chromatographic conditions and mass spectrometry conditions, recording detection results of the standard curve solutions with different concentrations to make a standard curve, and calculating the content of nitrite in the test solution by adopting a standard addition method according to the standard curve and the detection result of the test solution. The method disclosed by the invention can be used for rapidly determining trace nitrite in a drug sample, and can be used for determining the trace nitrite in pharmaceutical adjuvants and detecting the nitrite in drugs.

The present application relates to the technical field of Fischer-Tropsch synthesis wax analysis chemistry, and in particular to a method for detecting oxygen-containing compound components in Fischer-Tropsch synthesis wax by using solid-phase microextraction and comprehensive two-dimensional gas chromatography-mass spectrometry. The method comprises the following steps: first, performing solid-phase microextraction on a to-be-tested wax sample to obtain an extract rich in oxygen-containing compound components; and then using comprehensive two-dimensional gas chromatography-mass spectrometry to qualitatively determine the composition of the oxygen-containing compound components contained in the extract. In the method, the content of the oxygen-containing compound components is less than or equal to 7 wt% based on the total weight of the Fischer-Tropsch synthesis wax. The method has the characteristics of high throughput, high sensitivity and high accuracy, and solves the problems of difficult analysis and separation and difficult qualitative determination of alcohol, aldehyde, ketone, acid, ester and other oxygen-containing compound components in Fischer-Tropsch synthesis wax.

The invention discloses construction and application of a biosensor based on an efficient antifouling interface and dual signal amplification, and belongs to the technical fields of optical / electric analytical chemistry, biosensing and material science. According to the invention, the electrode interface is modified with polyaniline doped with polytannic acid as an antifouling film, so that non-specific adsorption of interfering biomolecules in a complex medium is resisted; euTb-MOF is prepared as an efficient luminous body, Tb < 3 + > can transfer energy of Tb < 3 + > and ligand to Eu < 3 + >, and effective signal self-enhancement is realized; according to the EuTb-MOF, the reduction of S2O8 < 2-> is promoted by utilizing the reversible conversion of valence states of Co < 2 + > / Co < 3 + > and Fe < 2 + > / Fe < 3 + > and the synergistic catalysis of Ag NPs, so that the electrochemical luminescence signal of the EuTb-MOF is further improved. Based on the above strategy, the constructed biosensor has high detection sensitivity and precision and long service life, the linear range is 10 fM to 100 nM, and the detection limit is as low as 3.62 fM.

The application relates to the technical field of analytical chemistry, in particular to a high-performance liquid chromatography analysis method for maleic acid olmutant, wherein the mobile phase in the high-performance liquid chromatography condition comprises mobile phase A and mobile phase B, the mobile phase A is a mixed solution, the mixed solution comprises trifluoroacetic acid, triethylamine and water, the volume fraction of trifluoroacetic acid in the mixed solution is 0.08-0.12%, the volume fraction of triethylamine is 0-0.3%, the mobile phase B is acetonitrile, and the volume ratio of the mobile phase A and the mobile phase B is 75:25-85:15. The trifluoroacetic acid and the triethylamine in the mobile phase are matched, the interaction between maleic acid olmutant and a chromatographic column is hindered, the peak shape is symmetrical, and peak tailing is significantly improved; meanwhile, the two can also form a buffer system; in addition, the trifluoroacetic acid can enhance the retention capacity of maleic acid olmutant on the chromatographic column and improve separation selectivity.

The invention relates to the technical field of analytical chemistry and mineral detection, in particular to a method for detecting potassium element in molybdenum concentrate, which comprises preparation, working curve solution preparation and inductively coupled plasma emission spectrometer detection. Through acid digestion, microwave treatment and instrument parameter optimization, the problem of matrix interference is solved, the detection sensitivity and efficiency are improved, the detection limit is greatly reduced, and the standard recovery rate is improved. The method is suitable for rapid quality monitoring of molybdenum concentrate, can be expanded to detection of other potassium-containing minerals, is combined with automatic equipment or an online monitoring system, improves the detection consistency and real-time performance, and has important application value.