Multiple channel micro-flow control chip, process for making same and use thereof

A microfluidic chip and multi-channel technology, applied in the direction of material inspection products, biological testing, material electrochemical variables, etc., can solve the problems that the advantages of electrochemical detection have not been fully utilized, and the limitations of single channels have not been broken through, so as to avoid High cost and interference problems, low cost, wide and deep channel effect

Inactive Publication Date: 2005-11-23
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] From the patent retrieval of microfluidic chips, it can be seen that although the chip materials and electrode materials used in the electrochemical detection of microfluidic chips are various, and the manufacturing method of the chip has made great progress compared with the traditional glass chip, it is still The limitation of single channel has not been broken through, therefore, the advantages of electrochemical detection in microfluidic chips have not been really brought into play

Method used

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  • Multiple channel micro-flow control chip, process for making same and use thereof
  • Multiple channel micro-flow control chip, process for making same and use thereof
  • Multiple channel micro-flow control chip, process for making same and use thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0051] The making of embodiment 1 A sheet

[0052] Sheet A is the substrate, and its production includes the production of the monocrystalline silicon positive mold, the separation and injection microchannel, and the working electrode. The specific production process is as follows:

[0053] (1) Mask production: drawing by CAD software figure 1 As shown in the design drawing, the length of the sampling channel (referring to the distance between the sample pool and the edge of the waste pool) is 2.828mm, the lengths of the four working electrode channels are 7, 13, 19, and 25mm respectively, and the distance between two adjacent working electrodes The horizontal distance between them is 6mm, the length of the separation channel is 50mm, the distance between the intersection point of the injection channel and the separation channel is 45mm from the right end of the separation channel, the vertical distance between two adjacent separation channels is 7mm, and the line width of al...

Embodiment 2

[0059] The making of embodiment 2 B sheet

[0060] Sheet B is a cover sheet, and its structure includes various reservoir holes, including 1 buffer pool, 4 sample pools, 4 waste liquid pools and 4 independent electrochemical detection pools. The specific production process is:

[0061] (1) The size of sheet B is 4.0×8.0 cm, and the thickness is 1-3 mm. In this embodiment, PMMA with a thickness of 2 mm is selected. The coverslip was washed with distilled water, soaked in 0.1mol / L NaOH solution for 30min, washed with distilled water and dried with nitrogen.

[0062] (2) Fabrication of the buffer pool: the cross section of the buffer pool is a parallelogram with a width of 5 mm and a length of 34 mm, and the angle between the length and width is 45 degrees. Since the requirement for the size of the buffer pool is not particularly precise, it is sufficient to use a diamond drill to drill holes in this embodiment. The height of the buffer pool is the thickness of slice B, ie 2mm...

Embodiment 3

[0064] Embodiment 3 The sealing of sheet A and sheet B and the making of electrodes

[0065] Use hot pressing method to seal A piece and B piece. The specific production process is:

[0066] (1) Ultrasonic cleaning of slices A and B, and air-dried.

[0067] (2) Align slice A and slice B accurately under the microscope so that the distance between the working electrode and the edge of the reservoir is about 30 μm. After aligning the position, fix and clamp the substrate and the cover with two pieces of glass and put them into the oven, program the temperature to 108°C, keep it warm for 2 minutes, and then program to cool down to 45°C.

[0068](3) Production of each electrode: the electrode used to provide high voltage in the buffer pool 1 is a Pt wire with a length of 10 mm and a diameter of 0.5 mm; the electrodes of the sample pool 2 and the waste liquid pool 3 are 5 mm long and 0.5 mm in diameter The detection cell 4 is a three-electrode working system, and the working ele...

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Abstract

The invention relates to a micro flow control chip in the leading edge field of analytical chemistry, which has the characters of small volume, fast analysis. Although micro flow control chip integrated with laser evoked fluorescence detection can do 384 channels high-pass measuring, it isn't reported multichannel electric chemistry measuring from now on. The invention provides a multichannel micro flow control chip, which adopts high molecular material polymethyl methacryate, and fabricates monocrystalline silicon core by using the technology of photo etching and etching, and microchannel adopts hot-press method or polymerization. It integrals the measuring electric pole channel onto the chip. It can fill functional material into electric pole and control the distance of working electric pole and separating channel.

Description

technical field [0001] The invention relates to the field of microfluidic chips, and develops a multi-channel microfluidic chip based on polymethyl methacrylate as a material and applied to electrochemical detection. The invention also provides the preparation method and application of the chip. Background technique [0002] Since the early 1990s when Manz et al. put forward the concept of "micro-total analysis system", the integration and portability of the analysis system has become the main development trend of modern analytical instruments. Compared with traditional capillary electrophoresis technology, microfluidic chip has the advantages of less sample consumption and fast separation speed, but at the same time, it has higher requirements for detection sensitivity and response speed. The most commonly used detection method is laser-induced fluorescence detection (LIF), but the instruments required for detection by this method are expensive, bulky and cumbersome. Elec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/27
Inventor 孔继烈彭友元周佳黄宜平
Owner FUDAN UNIV
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