69 results about "Total analysis system" patented technology

The development of miniaturized chromatographic systems localized within individual polymer microspheres and their incorporation into a bead-based cross-reactive sensor array platform is described herein. The integrated chromatographic and detection concept is based on the creation of distinct functional layers within the microspheres. In this first example of the new methodology, complexing ligands have been selectively immobilized to create “separation” layers harboring an affinity for various analytes. Information concerning the identities and concentrations of analytes may be drawn from the temporal properties of the beads' optical responses. Varying the nature of the ligand in the separation shell yields a collection of cross-reactive sensing elements well suited for use in array-based micro-total-analysis systems.

A novel filter-less separation technique for separating suspended particles from a solution is disclosed. More specifically, an on-chip bioparticle separator is disclosed, which relies on the differential force exerted by application of a series of high magnitude, short duration pressure pulses on bioparticles in suspension within microchannels, resulting in separation of suspended bioparticles. The filter-less separation technique is inherently suited to μTAS (Micro Total Analysis System) since it exploits uniquely microscale phenomena to achieve separation. The on-chip bioparticle separator can be easily integrated with a disposable biochip, can be fabricated using low-cost, rapid manufacturing techniques, and can provide high performance for separation of bioparticles without the use of specialized or expensive equipment. Embodiments of the present invention address a significant challenge in the development of disposable microfluidic biochips, specifically, providing a reliable solution for separating bioparticles in a microfluidic system that may be immediately applied for a variety of microfluidic biochip applications.

Disclosed is an one-dimensional biochip belonging to a serial analytic technique for micro-total analysis systems. The one-dimensional biochip is provided with a plurality of cellules on the micro-separation channel of micro flow control chip, microparticles modified by different biomolecules on surface are arranged in different cellules; in the gene or protein analysis, when the sample flows through the microchannel with a plurality of cellules, the microparticles can specifically identify and capture multiple target molecules, then a reagent with fluorescence labels can be introduced, and finally the microparticles will specifically combine with the fluorescence labels on surface to be detected by fluorescent imaging. The one-dimensional biochip provided by the invention not only has advantages of micro flow control technology and array analysis, but also improves the detection sensitivity and identification capability of target molecules, and provides an effective research measure for the gene and protein expression analysis at single cell level, and tumor research and drug screening.

The micromixer with overlapping-crisscross entrance incorporated with the grooved microchannel, is used effectively for mixing two or more fluid streams. The X-shape overlapping-crisscross inlet ports wherein two microfluidic channels contact over a small area, allow the fluid streams flow through and create the tumbling inside the micromixer. Then merging with some patterned grooves on the walls also induces swirling motion. As a result, the folding and stretching effects of the flow are augmented to amplify the fluid mixing of two or more streams of the inlet fluids within a relative short distance in the micromixer. All of the flow streams are actuated with either pressure driven by a syringe pump or capillary electrophoresis. The present invention is applicable for micro total analysis systems and drug delivery systems.

The present invention discloses a vortex-modulation based micromixer for enforced mass exchange. The micromixer of the present invention comprises a mixing chamber with grooves on one wall thereof and a special-shape barrier on another wall. As different fluids are injected into the mixing chamber respectively from two inlets of the micromixer, the grooves and barriers of the micromixer of the present invention create the constructive interferences to form the active-like agitation of the fluid. For every groove, the flux passed by can be increased via its high pressure gradient. Understandably, the mixing efficiency of the fluids can be greatly improved within a very short distance. At last, the outlet of the micromixer is located in the downstream of the mixing chamber and further is able to connect with other elements. The present invention is entirely a passive micromixer and no additional energy is required. The present invention can apply to a continuous chemical analysis, particularly to a lab-on-a-chip or a micro total analysis system.

The invention discloses a microfluidic microbead array chip and application thereof in virus analysis, and belongs to the field of biological application of a miniaturized total analysis system. The microfluidic microbead array chip consists of a microbead fixed microstructure array, a microbead transport channel, a reagent transport channel and a microstructure array coverage channel. When high-sensitive virus analysis is performed, virus molecules in a serum sample is prepared into a biotin doped target RNA sequence through PCR and in vitro transcription process, and flows to a testing areaof the chip to react with a functional microbead array; function modified microspheres can specifically identify and capture target sequences; a quantum dot labeled reagent are introduced; surfaces of micro-particles are combined with quantum dots because of specifically capturing; and detection and quantitation are performed by a fluorescence imaging method. The microfluidic microbead array chipprovided by the invention has the advantages of simple process, high detecting sensitivity and quick detection, and provides an effective studying and detecting means for high-sensitive virus analysis.

The invention involves the examination method of the analysis system of a kind of miniature whole chemistry and it corresponds of device. Design cover slice or slice of chip a thin film slice; Follow capillary passage in the outside side of film slice to should of the position sticks examination electrode tightly, examining electrode and the capillary passage in the chip not to get in touch with; Input high signal toward the examination electrode, examine the capillary aqua inside the passage at examine electrode the high electric conductivity of the district information, get separation diagram The advantage of the invention lies in a craft in brief and use convenience, can Be stirring up frequency lowery and stirring up electricity lowery function good under the work, safety and stability.

A system for analyzing a thin film uses an energy beam, such as a laser beam, to remove a portion of a contaminant layer formed on the thin film surface. This cleaning operation removes only enough of the contaminant layer to allow analysis of the underlying thin film, thereby enhancing analysis throughput while minimizing the chances of recontamination and/or damage to the thin film. An energy beam source can be readily incorporated into a conventional thin film analysis tool, thereby minimizing total analysis system footprint. Throughput can be maximized by focusing the probe beam (or probe structure) for the analysis operation at the same location as the energy beam so that repositioning is not required after the cleaning operation. Alternatively, the probe beam (structure) and the energy beam can be directed at different locations to reduce the chances of contamination of the analysis optics.

The invention belongs to the technical field of biomedicine application of micro total analysis systems, and particularly relates to an integrated multifunctional controllable cell control and analysis micro-fluidic chip and application of the chip. The integrated multifunctional controllable cell control and analysis micro-fluidic chip comprises an inertia focusing structure formed by continuous asymmetric bend channels, a laminar flow structure formed by a plurality of branch channels and a joining channel, a micro-fluidic control liquid drop forming structure and a micro-array capturing structure, wherein all the structures are connected through a channel, the inertia focusing structure, all branch channels of the laminar flow structure and the micro-fluidic liquid drop forming structure respectively have a solution injecting hole, and the micro-array capturing structure has a solution injecting hole and a waste solution outlet. According to the integrated multifunctional controllable cell control and analysis micro-fluidic chip, multiple functional structures are integrated in cell analysis for solving the problem that functions of high-flux analysis, trace analysis, cell controllable manipulation and the like in an existing cell analysis method are difficult to integrate, and the technical field is promoted to be further developed.

The invention provides a microfluidic substrate, a driving method thereof and a miniaturized total analysis system. The microfluidic substrate provided by the invention comprises a substrate and a plurality of detection modules located on the substrate, wherein each detection module comprises a switching unit located on the substrate and a driving electrode connected with a signal output terminalof the corresponding switching unit; each detection module further comprises a light sensitizing unit located on the substrate; and either first poles or second poles of the light sensitizing units are connected with signal output terminals of the switching units. According to the microfluidic substrate provided by the invention, the corresponding driving electrode and the corresponding sensitizing unit of each detection module share one switching unit, so that compared with the prior art, more detection modules can be arranged on the microfluidic substrate of a unit area, and thus, the realization of the high-resolution microfluidic substrate is facilitated.

A microchannel chip reaction control system of the present invention is characterized by being provided with a microchannel chip 1 having at least two microfluidic channels 1a and 1b for introducing reagent solutions A and B and a microfluidic reaction channel 1c formed by joining the two microfluidic channels 1a and 1b to each other, analysis means 2 for analyzing a product C obtained from the microfluidic reaction channel 1c, and control means 3 for controlling conditions for the reaction in the microchannel chip 1 on the basis of analysis results obtained from the analysis means 2.

A system including an inspection chip equipped with at least a pump connection having a flow path opening for interconnecting to a micro-pump, a flow path through which a fluid flows, and a fluid mixing section for joining and mixing two or more fluids, and including a system body equipped with at least a base body, a chip connection having a flow path opening for interconnecting to the inspection chip, a micro-pump unit in the base body including micro-pumps in substantially the same shape, a detection processor and a controller for controlling at least the functions of the micro-pump unit and the detection processor, and the joining amount ratio is adjusted to set the mixing ratio of two fluids to be joined in the fluid mixing section to be approximately m:n (not 1:1) by making the drive voltages of the micro-pumps substantially equal to each other.

The invention is a fiber embedded low-voltage drive capillary electrophoresis chip, relating to the analysis chemical micro total analysis system research technology field, particularly to the capillary electrophoresis chip production. The fiber embedded low-voltage drive capillary electrophoresis chip comprises the glass substrate and glass film; on the glass substrate embedded detection fiber for signal detection, and the low-voltage drive array electrode capillary electrophoresis chip and detection fiber are integrated into a chip, that is to say, the cruciform ditch on the glass substrate and platinum array electrode of detection fiber and the glass-film are combined together. On the glass substrate, by chemical corrosion, etch a laser importing fiber channel vertical to the separation ditch, and the fiber channel to transmit the excitation fluorescence. It reduces the injection and separation voltage, simplifies the detection optical path, narrows capillary electrophoresis chip detection system volume, improves detection sensitivity, and achieves the detector miniaturization and integration.

The invention discloses a planar passive type micromixer with stepped passages and an I-shaped baffles, and belongs to the field of microfluid mixing in micro total analysis systems and microfluidic chips and the relevant field thereof. The micromixer comprises a cruciform passage, a contraction passage and a plurality of mixing units, each mixing unit is formed by connecting a narrow slit straight passage, the stepped passage and the I-shaped baffle arranged in the stepped passage in sequence, the plurality of mixing units are connected in sequence between the contraction passage and the outlet of the straight passage and are gradually compressed after passing through the I-shaped baffles to promote mixing. Moreover, a jet impacts each of the I-shaped baffle to form a convection with an incoming flow, and collisions occur between the fluids to promote molecular diffusion. When the fluid passes through each of the I-shaped baffle, a separation vortex is easily formed on the back of theI-shaped baffle, which significantly improves the mixing effect of different liquids.

The present invention provides an ultrasonic micromixer which is used in fields of bio-MEMS (micro-electro-mechanical-system) and μ-TAS (total analysis system) and radiates ultrasonic waves using a piezoelectric device in a direction perpendicular to a horizontal mixing interface between a plurality of sample fluids flowing in a chamber, so as to accelerate the mixing of the sample fluids, thus producing an evenly mixed sample. The ultrasonic micromixer includes a plurality of independent inlets, and a plurality of guide channels which are coupled to the inlets at different heights. The ultrasonic micromixer further includes the chamber to allow the plurality of sample fluids, discharged from the guide channels, to mix with each other while flowing through the chamber, and an outlet to discharge the mixed sample fluids. The ultrasonic micromixer further includes a vibration plate which is attached to a lower portion of the chamber, and a piezoelectric device which is attached to a lower surface of the vibration plate to radiate ultrasonic waves in a vertical direction. Therefore, the ultrasonic micromixer achieves miniaturization, accuracy and integration of devices used in the fields of the bio-MEMS and μ-TAS.

The invention relates to the technical field of bioscience micro-total analysis systems, in particular to a micro-fluidic chip bonding method and a micro-fluidic chip. The method includes: providing amicro-fluidic chip substrate, wherein the upper surface of the substrate is provided with a microchannel structure; arranging ultrasonic bonding energy conducting ribs at the edge around the microchannel structure of the upper surface of the substrate or around a position, corresponding to the microchannel structure of the substrate, of a cover plate; adhering a surface pasting double-side adhesive tape on the substrate or the cover plate to glue the cover plate with the substrate; performing ultrasonic bonding on the substrate and the cover plate to enable first-step bonding; applying pressure to fix the chip or hot-pressing the same to enable the substrate and the cover plate which are pasted by the double-side adhesive tape to be firmly bonded. By the method, bonding stability of the micro-fluid chip can be ensured, and own physiochemical properties of the micro-fluidic chip can be avoided being affected, so that detection results are not interfered.

The present embodiments describe a method that integrates a magnetostrictive sensor with driving and detecting elements into a microfluidic chip to detect a chemical, biochemical or biomedical species. These embodiments may also measure the properties of a fluid such as viscosity, pH values. The whole system can be referred to lab-on-a-chip (LOC) or micro-total-analysis-systems (μTAS). In particular, this present embodiments include three units, including a microfluidics unit, a magnetostrictive sensor, and driving/detecting elements. An analyzer may also be provided to analyze an electrical signal associated with a feature of a target specimen.

The invention belongs to the field of micro-electro-mechanical systems, and relates to a micro-total analysis system chip used for integral detection of heavy metal ions. Two liquid inlets of the chip communicate with a micro-mixer through a Y-shaped liquid inlet channel, the other end of the micro-mixer is connected with the liquid inlet end of a solid phase extraction column, the solid phase extraction column is filled with a selective adsorbent, the liquid outlet end of the solid phase extraction column is connected with a detection cell, the bottom of the detection cell is in contact with a three-electrode ampere detection sensor arranged in a sensor bin, the three-electrode ampere detection sensor is connected with an electrochemical workstation through an electrode lead wire arranged in an electrode lead wire bin and an Ag/AgCl electrode arranged in an internal filling solution cell, the detection cell communicates with a waste liquid channel through a waste liquid cell, and the waste liquid cell is provided with a liquid outlet. The micro-total analysis system chip has the advantages of small size, high sensitivity, good detection reappearance and stability, high integration degree and recoverable main components.