Optical fibre embedded low-voltage actuated capillary tube electrophoresis chip

Abstract

The invention is a fiber embedded low-voltage drive capillary electrophoresis chip, relating to the analysis chemical micro total analysis system research technology field, particularly to the capillary electrophoresis chip production. The fiber embedded low-voltage drive capillary electrophoresis chip comprises the glass substrate and glass film; on the glass substrate embedded detection fiber for signal detection, and the low-voltage drive array electrode capillary electrophoresis chip and detection fiber are integrated into a chip, that is to say, the cruciform ditch on the glass substrate and platinum array electrode of detection fiber and the glass-film are combined together. On the glass substrate, by chemical corrosion, etch a laser importing fiber channel vertical to the separation ditch, and the fiber channel to transmit the excitation fluorescence. It reduces the injection and separation voltage, simplifies the detection optical path, narrows capillary electrophoresis chip detection system volume, improves detection sensitivity, and achieves the detector miniaturization and integration.

Description

technical field [0001] The invention relates to the research technical field of micro-total analysis system in analytical chemistry, in particular to the manufacture of a capillary electrophoresis chip, which realizes the miniaturization and integration of a detector by using a relatively low sample injection and separation voltage and an embedded detection optical fiber. Background technique [0002] The integrated capillary electrophoresis chip is an important branch in the development of micro-device systems. It combines biotechnology, microelectronics technology and MEMS technology to integrate various channels, electrode slots, detection flow cells, etc. into the same substrate. Electrophoresis instrument is a multi-functional micro analysis device with fast, high efficiency, high sensitivity and low sample volume. Since 1991, the international development of integrated capillary electrophoresis chip manufacturing technology and its application in rapid analysis in biol...

AI Technical Summary

Problems solved by technology

[0005] At present, there are few domestic and foreign reports on capillary electrophoresis chips that replace high separation voltages with low voltages. Laser-induced fluorescence detection using embedded optical fibers is still in the initial stage of research, and has not yet been commercialized.

Images