The invention relates to a tube orifice diameter variable plug-in sampling instrument which structurally comprises conical tubes, straight bobbins, a steady flow buffer tube, a core tube, a diversion pressure regulating valve, a pressure gauge, a sampling hose, a concentric flow regulator and the like. The instrument changes the composition, the structure and the operating principle of the original organic polluted water sampling device, has the characteristics of small size, light weight, wide application scope, convenience in carrying and mounting, simplicity in operation, safety in use, prevention of a pollution link in a collection process of the sample and the like, improves the fidelity of the collected sample, lowers the collecting cost of the sample, and ensures the safety of personnel and equipment, and the universality of the sampling instrument is improved.