115 results about "Micro analysis" patented technology

An inverted microwell (102) provides rapid and efficient microanalysis system (100) and method for screening of biological particles (128), particularly functional analysis of cells on a single cell basis. The use of an inverted open microwell system (102) permits identification of particles, cells, and biomolecules that may be combined to produce a desired functional effect also functional screening of secreted antibody therapeutic activity as well as the potential to recover cells and fluid, and optionally expand cells, such as antibody secreting cells, within the same microwell.

The invention relates to a visual simulation shale micro-crack plugging capacity test system and a visual simulation shale micro-crack plugging capacity test method. Displacement evaluation experiment is performed on a single or combined micro-crack rock sample by a solution or a drilling fluid which contains the plugging agents with different concentrations and different types at different experiment conditions, such as pressure differences and times, indexes, such as the invasion depth, are compared, the formation conditions of inner mud cakes in drilling are directly described, and microscopic observation and analysis are performed on percolation substances invade into the micro-cracks by utilizing amplification imaging instruments, such as a high-definition microscope, so that the plugging mechanisms and the effects of various drilling fluid plugging agents and drilling fluids are analyzed and researched intuitively and microscopically, a proper drilling fluid plugging agent is selected preferably, a drilling fluid system formula is optimized, the problem about the experimental conditions that the visual simulation evaluation cannot be performed on shale micro-crack plugging in the past is solved, and a new evaluation experimental research means is provided for related research and production in the technical field of fractured shale strata borehole wall stability in petroleum engineering.

The invention belongs to the technical field of biological analysis and detection, in particular relates to a biological detection microfluidic chip based on immune reaction and a preparation method thereof. The chip takes optically transparent polydimethylsiloxane as a material and comprises a sample channel layer, a valve control layer and a substrate layer. The chip contains a sample enrichment and immunoassay module which comprises one or a plurality of nanolitre-size immune chromatographic column micro-analysis rooms, wherein each analysis room is fixed with antibody protein or antigen and is used for realizing rapid field detection of a plurality of antigen samples, such as bacteria, viruses and various toxins. The invention has the characteristics of fast speed, high efficiency, convenient carrying, low cost and easy automation control, and is capable of finishing automatic signal acquisition, remote transmission and signal analysis and suitable for rapid field detection and large-range remote detection of a plurality of antigens with the existence of commercial antibodies.

A micro integrated analysis system includes a testing chip, system main body and heat insulating section. The chip has a pump connecting section to communicate with micro pumps; a mixing flow path for mixing a specimen and reagent and for reaction processing by heating; and a testing flow path for performing a predetermined test of a mixed solution heated and processed for reaction. The system main body has a holding section to hold the testing chip; micro pumps for injecting a specimen and reagent; a heating section for heating the mixing flow path; and a detection section for performing a test in the testing flow path. The heat insulating section insulates flow path portions continuing from both an inlet end and outlet end of a heated flow path area of the mixing flow path, from heat of the heating section so as to prevent a rise in temperature.

The invention relates to a composite material of monolayer graphite and polymers, as well as a preparation method thereof. The composite material takes the monolayer graphite and the polymers as raw materials which are well mixed, wherein the weight portions of the raw materials are: 0.1 to 100 portions of the monolayer graphite and 0.1 to 100 portions of the polymers or other matrix materials. The preparation method is characterized by utilizing the excellent conductivity and large length-diameter ratio of the monolayer graphite to prepare conducting composite materials. Micro analysis proves that the monolayer graphite evenly disperses in the matrix materials, and a conducting network can be formed only by adding a small amount of the monolayer graphite, so as to obtain the conducting composite material with a plurality of forms. The composite material simultaneously has high strength and modulus, and is used in building, mechanical, aerospace and other special environments. In addition, as the monolayer graphite has excellent heat-conducting property, the composite material has the advantages of heat dissipation convenience and the like, and is expected to be applied to the precision instrument, microelectronic and other fields.

System and method for preparing a sample for micro-analysis, comprising: (a) sample precursor holding unit, for supporting and holding a sample precursor; (b) transporting and positioning unit, for transporting and positioning the sample precursor holding unit; (c) optical imaging unit, for optically imaging, recognizing, and identifying, target features on the sample precursor, and for monitoring the sample preparation; (d) picking and placing unit, for picking and placing the sample precursor and system components from initial positions to other functionally dependent positions; (e) micro-groove generating unit, for generating at least one micro-groove in a surface of the sample precursor, wherein the micro-groove generating unit includes components for controlling formation of each micro-groove in the surface; and (f) cryogenic sectioning unit, for cryogenically sectioning the sample precursor to a pre-determined configuration and size, for forming the prepared sample. Optionally, includes a micro-mask adhering unit, and a macro-mask adhering method.

The invention belongs to the technical field of micro analysis, in particular to an electrochemical sensor chip based on the digital microfluidic technology. The sensor chip comprises a digital microfluidic device and a three-electrode electrochemical device, wherein the three-electrode electrochemical device is stacked on the digital microfluidic device; the digital microfluidic device comprises a bottom layer and an upper cover, wherein the bottom layer comprises a first substrate, a first insulating layer, a first electrode layer, a second insulating layer and a first drainage layer in sequence from bottom to top; the upper cover comprises a second drainage layer, a second electrode layer, a third insulating layer and a second substrate in sequence from bottom to top; when the first substrate and the second substrate are made of insulating materials, the first insulating layer and the third insulating layer are dispensed; three electrodes include a counter electrode, a working electrode and a reference electrode; and the three electrodes and the first drainage layer are positioned on the same plane and surrounded but not covered by the first drainage layer. The electrochemical sensor chip can realize the delivery and the online detection of various required liquid samples.

A microscopic analysis apparatus for analysis of infrared single beam spectrum is composed of an interference light source unit, a measuring unit, and a signal processing unit. The measuring unit is configured such that a semi-spherical prism made from germanium, an incident angle varying device composed of a pair of opposed parabolic mirrors, and a detector for sensing light totally reflected from the surface of a sample are provided in an enclosed sample chamber. According to the method using the analysis apparatus, a profile of a concentration of an organic lubricant contained in a sample in the depth direction of the sample is measured by bringing the surface on the magnetic layer formation side of a floppy disk as the sample into press-contact on the bottom surface of the prism at a low pressure, scanning an incident angle of an infrared light ray on the prism, and analyzing the spectrum of the infrared light ray totally reflected from the surface of the sample.

The invention discloses a device and a method for transmission-scattering imaging of a nanometer liquid sample in a scanning electron microscope, belongs to the technical field of electronic micro-analysis devices, and particularly relates to the technical field of reflection and transmission-scattering imaging of the nanometer liquid sample in the scanning electron microscope (SEM). The device and the method are characterized in that a transmission-scattering electronic detector (TSD) is mounted in a sample chamber of the SEM and 5-10 mm under a thin window of the nanometer liquid sample; under the condition that the incident electron energy is 15-30 keV, a transmission-scattering electron image with a resolution ratio of several nanometers, an amplification factor of 50-100000 times and high image contrast can be formed for several to 500 nanometers of nano-particles in a sealed liquid; the TSD is suitable for being mounted in various SEMs, and can be cooperatively used with a secondary electron detector (ETD), an energy disperse spectroscopy (EDS) and a cathode fluorescence spectrometer (CL) for observation of such nanometer-like materials as metal, semiconductors, inorganic matters, organic matters, composites and biological matters.

The invention relates to a device for analyzing and sieving liquid drops based on an integrative sampling probe of a chip. A sampling passage, a reagent passage and a liquid carrying passage are arranged in a sampling probe of a micro-analysis chip; the sampling passage, the reagent passage and the liquid carrying passage are communicated by a T type passage structure, a Y type passage structure or a cross type passage structure. The device is used for a liquid drop analysis system based on a micro-analysis chip and overcomes the limitations that most of current liquid drop generation systems only can continuously generate liquid drops with single formation, are not easy to change the size and the formation of the liquid drops at any moment and are difficult to realize the replacement of test samples with different varieties. The invention has the advantages of high integration degree, convenient operation of sample exchange, simple device, low cost, long service life and the like and provides a novel introduction, analysis and sieving mode of test samples for the liquid drop analysis system of the micro-analysis chip at present.

The invention belongs to the technical field of biological chips, concretely relates to a novel electrochemical sensor chip with a digital microfluidic technology. The chip is sequentially composed of three parts from bottom to top: a droplet control unit, a droplet passage and an electrochemical unit, wherein the droplet control unit is composed of a first substrate, a first insulation layer, a first electrode layer, a second insulation layer and a first hydrophobic layer from bottom to top; the electrochemical unit is sequentially composed of an electrochemical electrode group, a second hydrophobic layer, a second electrode layer, a third insulation layer and a second substrate from bottom to top; when the first substrate and the second substrate are insulation materials, the first insulation layer and the third insulation layer are left out; the electrochemical electrode group comprises a counter electrode, a working electrode and a reference electrode and covers the lower surface of the second hydrophobic layer; the droplet passage is an air gap in the droplet control unit and the electrochemical unit. In the invention, the advantages of a digital microfluidic technology and an electrochemical sensor technology are combined and a novel micro-analysis device is provided for biochemical analysis; and therefore, the sample demand is decreased, the detection speed is increased, the conveying of various liquid samples needed in detection can be realized and the on-line detection is realized.

A testing chip that analyzes a specimen includes: a reagent storage section; a mixing and reaction flow channel to perform a series of operations to mix a specimen and aqueous reagent, make the specimen and reagent react with each other, and detect the reaction; and a liquid feed control section provided between an outlet flow channel of the reagent storage section and the inlet of the mixing and reaction flow channel. Herein, aqueous reagent, lipophilic liquid, and aqueous liquid having greater surface tension than that of the aqueous reagent are disposed in the reagent storage section in this order toward the outlet flow channel, the aqueous liquid being stored in contact with the liquid feed control section; and aqueous liquid passes the micro flow path of the liquid feed control section by applying a liquid feed pressure higher than or equal to a predetermined pressure to the reagent storage section.

This invention discloses one current load metal film stretching stress value test describing method, which comprises the following steps: processing current load and micro force stretching on the good metal film of interface; during this process recording the metal film stress curve and resistance change curve; acquiring the crack percentage stress curve through micro analysis by observing metal film. The current load metal film and flexible base board system invalid threshold system is composed of three threshold stress, such as the stress from resistance change to stress curve from linear phase to non-linear conversion; micro crack percentage stress curve back reducing as zero theory into threshold extension stress; resistance change to stress curve resistance runaway time into threshold crack unstable stress.

The invention discloses a method for rapidly measuring and finely classifying full-form crystal grains of a steel material. The method comprises the following steps: performing the operations of filtering, denoising, gray level correcting and binary segmentation treatment on an original image in sequence, and reducing a target crystal grain binary image; setting a scale for the reduced image, performing area calibration on target crystal grains respectively, and respectively extracting form characteristic parameters of each target crystal grain, wherein the form characteristic parameters include the area, the circumference, the grain size, the length-width ratio, the circularity degree, the form coefficient and the transgranular polar angle; distinguishing equiaxial crystal grains from non-equiaxial crystal grains according to a primary threshold of the circularity degree and a primary threshold of the form coefficient; distinguishing circular-like and polygon crystal grains from the equiaxial crystal grains according to a secondary threshold of the circularity degree and a secondary threshold of the form coefficient; and identifying strip-like and strip crystal grains and thick needle and small needle shaped crystal grains from the non-equiaxial crystal grains according to the length-width ratio and primary and secondary thresholds of the transgranular polar angle. The method is high in measurement precision, and the efficient and precise method is provided for rapid and fine microanalysis on the full-form crystal grains of the steel material.

The invention discloses an evaluation method of tribology performance of self-lubricating joint bearings. The method includes the steps of: first, recording and macroscopically analyzing such dynamic data as friction coefficient and wearing capacity in a frictional wear test of the self-lubricating joint bearings; second, using a scanning electronic microscope, an energy spectrum analyzer and the like to microscopically analyze the friction surface of the self-lubricating joint bearings as well as the frictional wear mechanism, and further using a microphenomenon to verify the correctness of variation tendency of macroscopic frictional wear performance; and finally, adopting a three dimensional surface topography contourgraph to conduct three dimensional topography scanning on the friction surface of the self-lubricating joint bearings and quantitatively analyzing the wear status of the self-lubricating joint bearings by combining a three dimensional surface topography diagram and three dimensional topography parameter. The invention adopts three different methods to evaluate the tribology performance of the self-lubricating joint bearings, and all the methods are verified mutually, form cumulative progression and proceed from qualitatively to quantitatively, thus accurately, completely and roundly evaluating the tribology performance of the self-lubricating joint bearings.

A micro-analysis method for a transformer device based on a BP nerve network and a manual shoal comprises the following steps: obtaining the data such as furfural, brackish water, disruptive voltage and acid value; optimizing the weight and the threshold of the BP nerve network by utilizing a manual shoal optimizing AFSO algorithm, so as to define the weight and the threshold; extracting the state micro-analysis result of a breakdown case base of the transformer device to train the BP nerve network; and inputting the newly obtained furfural, brackish water, disruptive voltage, acid value, hydrogen content, acetylene content, methane content, ethylene content, commissioning time, breakdown frequency in the recent three years, and breakdown grade in the recent three years into the BP nerve network for micro-analysis of the state of the transformer device, so as to define the state of the transformer device. The method has the advantages of scientificity, efficiency and accuracy.

The invention belongs to the technical field of micro analysis chips and in particular relates to an electrochemical integrated sensing chip based on a digital micro-fluid technology. The chip takes the digital micro-fluid technology based on electrowetting on dielectric as the basis, and comprises two parts, namely a digital micro-fluid module for a double-layer pole plate for performing automatic operations, such as generation, transport, mixing and separation of micro-scale droplet samples, and an integrated electrochemical detection module for a single-layer pole plate, wherein in the part, the micro electrochemical system is integrated on a digital micro-fluid chip of a lower pole plate, so that the transport and real-time electrochemical detection of the droplet samples are finished; moreover, the droplet samples can be freely transported on the two parts of the chip, so that different functions are realized. The electrochemical integrated sensing chip is organically combined by the digital micro-fluid chips of the single-layer pole plate and the double-layer pole plate, the structure is novel, micro rapid and sensitive detection can be realized, and the application range of the electrochemical sensing and digital micro-fluid technology is greatly widened.

The invention belongs to the technical field of biological analysis detection, in particular to a microfluidic chip for cotinine quick detection and a preparation method thereof. In the chip, optical transparent polydimethylsiloxane and the like can be used as materials by adopting a molding method; and the chip mainly comprises a sample reaction microchannel layer, a valve control layer and a substrate layer. The chip comprises a sample enriching and immune analysis module and a signal acquisition module, wherein the sample enriching and immunity analysis module consists of a plurality of immune chromatographic column micro-analysis chambers with nanoliter volumes, and each analysis chamber fixes cotinine antibody protein or antigen to realize quick detection on the cotinine in samples from different sources. The chip has the characteristics of quickness, high efficiency and low cost, is convenient to carry, is easy to control automatically, can complete automatic signal acquisition, remote transmission and signal analysis, and is suitable for quick detection on the ingredient in the air and medical clinical detection.

The invention discloses a micro-analysis method applied to the rock material I type crack initiation and expansion process. The method comprises the following steps that 1, a micro model for SiO2 crack initiation is built; 2, the working condition is set, and the crack expansion process is simulated and analyzed, and the average stress and the total crack length of the area at one end of a crack are calculated; 3, a crack stress intensity factor is calculated; 4, crack initiation driving force is calculated; 5, a calculation method of the average speed of microscopic crack expansion is built;6, influence rules of different conditions to the crack initiation and expansion process are analyzed. The method has the advantages that it is first put forward that molecular dynamics is adopted forsimulating the rock material microcrack initiation and expansion process, the stress intensity factor calculation method under microscopic molecular dynamics simulation and crack expansion speed calculation method in microscopic simulation are built, and a theoretical basis is provided for deeply analyzing the whole microcrack expansion process such as rock brittle fracture and creep and mechanism study.

The invention discloses coal reservoir fracture observation submicroscopic analysis technology which is used for observing fractures of coalbed methane reservoir stratums. The technology includes preparing a lump coal polished section. The lump coal polished section is photographed through a professional camera. The photographed pictures are numbered according to the sequence and stored. And then, the pictures are partially enlarged in a computer and the fractures are counted. The professional camera is arranged on a fixed support and is aligned with the lump coal polished section, and a fixed focus macro lens is installed on the camera. A reference proportional scale is placed next to the lump coal polished section, and the lump coal polished section and the reference proportional scale are placed in the same picture in the photographing process. The pictures of the lump coal polished section are opened through image editing software in the computer, and then the positions with the fractures in the pictures are marked and recorded. Compared with the existing coal reservoir naked-eye analysis technology and the microscopic analysis technology, the coal reservoir fracture observation submicroscopic analysis technology is capable of carrying out submicroscopic analysis on characteristics which can not be analyzed through naked eyes and a microscope, and is capable of improving coal reservoir observation efficiency, reducing manpower cost and increasing observation reliability.

The invention provides a coronary artery image analysis method and a data structure. The coronary artery image analysis method comprises the steps of S1, performing preprocessing on reference human body data and detected human body data; S2, performing three-dimensional analysis and two-dimensional analysis on the reference human body preprocessed data and the detected human body preprocessed datato obtain reference human body macro and micro analysis data and detected human body macro and micro analysis data, and associating the reference human body analysis data and the detected human bodyanalysis data; and S3, synchronously displaying the reference human body analysis data and the detected human body analysis data. The beneficial effect is that users can simultaneously compare and observe two-dimensional image data, three-dimensional models and images in blood vessel cavities of coronary arteries of a reference human body and a detected human body so as to achieve better coronaryartery teaching effects.

The invention belongs to the technical field of nanometer materials and discloses a 3DTEM and 3DAP multi-scale characterization universal sample table which comprises a sample table base; the tail endof the sample table base is in a rectangular shape, and the front end is in a shape of a swallow tail and form a right angle with the back end; the tail end of the sample table base is provided witha round hole; an outer copper pipe is welded to the front end of the sample table base; the front end of the outer copper pipe is sleeved with an inner copper pipe; the outer copper pipe is fixed withan inner copper pipe through a friction force; and a three-dimensional atom probe needle-like sample is clamped with the front end of the inner copper pipe. The 3DTEM and 3DAP multi-scale characterization universal sample table provided by the invention is ingenious in design, easy to disassemble, convenient to operate and relatively low in cost, can be repeatedly utilized, is easily combined with two three-dimensional atom probe needle-like sample preparation methods, enables sample preparation and electronic micro-analysis to be efficiently connected together and chemical component information of a three-dimensional atom probe and microstructure information of a three-dimensional transmission electron microscopy to be fully combined, has better applicability and can be more generally applied.

A method for analysis of a cell structure includes a macroanalysis step and a microanalysis step. The macroanalysis step includes replacing a cell structure or a part of the cell structure with an anisotropic solid body having property values of equivalent rigidity characteristics, creating a finite element model of the anisotropic solid body based on the property values, applying an internal temperature distribution or an external pressure to the finite element model of the anisotropic solid body, determining the stress distribution in the anisotropic solid body, and selecting an aimed cell structure of which the stress should be calculated as a cell structure based on the stress distribution. The microanalysis step includes creating a finite element model of the aimed cell structure, and determining the stress distribution in the aimed cell structure based on the finite element model of the aimed cell structure. The structural analysis method is a means for analyzing the stress distribution in the cell structure due to the internal temperature distribution or external pressure which can be realized by using general-purpose computer software and hardware without performing a simulation test and making a large investment.

The invention belongs to the technical field of integrated micro analysis chips and in particular relates to an integrated electrochemical sensing chip. The chip comprises two parts, namely a digital micro-fluidic driving module arranged on a lower pole plate of the chip for generating, transporting, mixing and separating micro-scale droplets, so that liquid samples can be automatically controlled; and an integrated electrochemical sensing module arranged on an upper pole plate of the chip, so that micro real-time and rapid electrochemical detection can be performed. The chip has the advantages of novel design, high integration level, convenience in operation, high degree of automation and the like and can be applied to the fields of automatic micro analysis and lab-on-a-chip.

An electrochemical detector integrated on a capillary electrophoresis chip according to the present invention includes: a first substrate having a microchannel; a second substrate adapted to mate with the first substrate and having at least one peripheral electrode for conducting electrophoresis of a sample injected along the microchannel of the first substrate, in which a separation channel is formed along the microchannel by bonding the first substrate with the second substrate; a first electrode, made of indium tin oxide (ITO), formed on the first substrate to be positioned over the separation channel; and a second electrode, made of indium tin oxide (ITO), formed on the second substrate to be positioned under the separation channel, and spaced apart from the first electrode at a predetermined interval, wherein the first electrode and the second electrode constitute a detector to measure electrical characteristics of the sample passing along the separation channel. According to the present invention, since the specific characteristics of a sample can be evaluated by measuring the electrical or genetic characteristics of the sample flowing along the microchannel formed in a chip using a detector, a chip for a micro-analysis system having a simple structure can be realized.

The invention relates to a method for measuring seven functional components of liquorice in cosmetics. The method comprises the following steps: adding 60 to 80 percent of methyl alcohol into a sample to be tested, performing repeated ultrasonic extraction and centrifugation, combining centrifugal supernate, adding the supernate into a rotary evaporating flask, performing rotary evaporation till constant volume is not exceeded, transferring the methyl alcohol and the sample into a graduated tube, metering the volume through methyl alcohol, filtering through a filter membrane, performing liquid chromatography measurement on filtrates, adopting a diode array detector, using octadecylsilane chemically bonded silica as a filler column, detecting at 190 to 400 nm, using an acetonitrile-phosphoric acid solution as a flowing phase, performing gradient elution, and collecting seven functional components which are liquiritin, isoliquiritin, glycyrrhizin, dipotassium glycyrrhizinate (glycyrrhizic acid), licochalcone A, glabridin and glycyrrhetinic acid. The method is high in resolution ratio and sensitivity, good in selectivity, short in time, simple in sample treatment method, and suitable for the micro analysis of seven functional components of liquorice in the cosmetics, and fills a gap of the measurement of seven functional components of liquorice in the cosmetics.

The present invention relates to the low temperature packing method of glass substrate chip, and features that in the condition of existing hydrated layer in the packed surface of the micro analysis chip with glass substrate and in the ambiance of low temperature (40-200 deg.c) or room temperature (0-40 deg.c), the micro analysis chip with glass substrate is packed through low temperature heating or simple setting. The present invention has the advantages of simple and feasible technological process, low temperature or room temperature packing, and packing performance reaching the requirement of conventional electrophoretic separation experiment.

The invention belongs to the technical field of biologic analysis and detection, and in particular relates to a microfluidic chip for rapid detection of saxitoxin and a method for preparing the same. The chip is made of the material such as optically transparent dimethyl silicone polymer by the molding method and mainly comprises a sample reaction micro-channel layer, a valve control layer and a substrate layer. The chip comprises a sample enrichment and immunity analysis module and a signal acquisition module, wherein each module consists of a plurality of paralleled immunity chromatographiccolumn micro analysis chambers of nanoliter volume; and saxitoxin antibody protein or antigen is fixed in each analysis chamber so as to realize rapid on-site detection of the saxitoxin in the sample. The chip can perform automatic signal acquisition, remote transmission and signal analysis due to the characteristics of high speed, high efficiency, convenient carrying, low cost and easy automation control and is suitable for on-site rapid detection of the saxitoxin in the water environment and remote detection in a large range.

This invention relates to a nanowire-assisted method for mass spectrometric analysis of a specimen. More specifically, by using nanowire which can fix a specimen and perform desorption / ionization of the specimen while effectively transferring laser energy to the specimen to be irradiated, thereby enabling to perform mass spectrometric analysis without using a matrix solution. This invention, by effectively performing desorption / ionization of a specimen using the above-mentioned nanowire, enables to effectively perform qualitative-, quantitative-, and micro-analyses of specimens as well as low molecular weighted specimens. Further, this invention enables to the typical device of mass spectrometric analysis used in MALDI-T of MS. In particular, this invention can perform mass spectrometric analysis of a specimen with molecular weight of less than 1,000 Da and perform quantitative analysis by fixing a specimen with a predetermined area.