Device for analyzing and sieving liquid drops based on integrative sampling probe of chip

A screening device and probe technology, applied in sampling devices and other directions, can solve the problems of time-consuming and laborious, and increase the difficulty of microfluidic manipulation, and achieve the effect of reducing the difficulty of manipulation and high integration.

Active Publication Date: 2010-04-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method requires manual preparation of the droplet loader, which is time-consuming and laborious, and requires the interface between the droplet loader and the chip to transfer the droplet into the chip.
In addition, under the pressure...

Method used

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  • Device for analyzing and sieving liquid drops based on integrative sampling probe of chip
  • Device for analyzing and sieving liquid drops based on integrative sampling probe of chip
  • Device for analyzing and sieving liquid drops based on integrative sampling probe of chip

Examples

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Embodiment 1

[0036] figure 1 It is a schematic top view of a droplet analysis and screening device based on a chip integrated sampling probe established according to the present invention. An integrated sampling probe 2 is processed on the microchip 1, and the sampling probe 2 integrates a sampling channel 4, two reagent channels 6, and a carrier liquid channel 8, so that it has the functions of sample introduction, quantitative addition of reagents, and liquid generation. The function of the drop reactor.

[0037]Before the droplet analysis and screening device is used, all microchannels in the microchip 1 and the outer wall surfaces of the sampling probe 2 need to be treated with hydrophobic or hydrophilic treatment. Fill the channel of the chip with carrier solution. The syringe used to drive the carrier liquid is filled with the carrier liquid, and the syringe is connected to the inlet 12 of the carrier liquid channel 8 through a pump tube to provide positive pressure driving. The s...

Embodiment 2

[0042] Figure 5 It is a schematic top view of an integrated sampling probe according to yet another embodiment of the present invention. Different from Example 1, when the droplet reactor only needs one reagent to participate in the reaction, only one reagent channel 6 needs to be processed in the sampling probe 2, so that the screening sample and the reagent form a confluence and generate a droplet reaction device. This can further reduce the size of the sampling probe 2 .

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Abstract

The invention relates to a device for analyzing and sieving liquid drops based on an integrative sampling probe of a chip. A sampling passage, a reagent passage and a liquid carrying passage are arranged in a sampling probe of a micro-analysis chip; the sampling passage, the reagent passage and the liquid carrying passage are communicated by a T type passage structure, a Y type passage structure or a cross type passage structure. The device is used for a liquid drop analysis system based on a micro-analysis chip and overcomes the limitations that most of current liquid drop generation systems only can continuously generate liquid drops with single formation, are not easy to change the size and the formation of the liquid drops at any moment and are difficult to realize the replacement of test samples with different varieties. The invention has the advantages of high integration degree, convenient operation of sample exchange, simple device, low cost, long service life and the like and provides a novel introduction, analysis and sieving mode of test samples for the liquid drop analysis system of the micro-analysis chip at present.

Description

technical field [0001] The field of the invention relates to the field of droplet analysis, in particular, a droplet analysis and screening device based on a micro-analysis chip integrated sampling probe and a sample introduction method thereof. Background technique [0002] In the past ten years, microfluidic technology has developed towards the direction of high integration, high throughput, low consumption, and low cost, and has embarked on a road different from microarray and multiwell plate array technology. In particular, the emergence of droplet-based microfluidic analysis technology has fully demonstrated the charm of microfluidic systems for manipulating microvolume fluids in microchannels. The droplet-based microfluidic analysis system uses immiscible liquid phases or gas phases as intervals between nL to pL-sized droplets to form independent reaction vessels, which have been successfully used in chemistry, physics, and biology. fields of science and medicine. Co...

Claims

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Application Information

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IPC IPC(8): G01N1/14
Inventor 方群孙蒙
Owner ZHEJIANG UNIV
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