Device for analyzing and sieving liquid drops based on integrative sampling probe of chip

Abstract

The invention relates to a device for analyzing and sieving liquid drops based on an integrative sampling probe of a chip. A sampling passage, a reagent passage and a liquid carrying passage are arranged in a sampling probe of a micro-analysis chip; the sampling passage, the reagent passage and the liquid carrying passage are communicated by a T type passage structure, a Y type passage structure or a cross type passage structure. The device is used for a liquid drop analysis system based on a micro-analysis chip and overcomes the limitations that most of current liquid drop generation systems only can continuously generate liquid drops with single formation, are not easy to change the size and the formation of the liquid drops at any moment and are difficult to realize the replacement of test samples with different varieties. The invention has the advantages of high integration degree, convenient operation of sample exchange, simple device, low cost, long service life and the like and provides a novel introduction, analysis and sieving mode of test samples for the liquid drop analysis system of the micro-analysis chip at present.

Description

technical field [0001] The field of the invention relates to the field of droplet analysis, in particular, a droplet analysis and screening device based on a micro-analysis chip integrated sampling probe and a sample introduction method thereof. Background technique [0002] In the past ten years, microfluidic technology has developed towards the direction of high integration, high throughput, low consumption, and low cost, and has embarked on a road different from microarray and multiwell plate array technology. In particular, the emergence of droplet-based microfluidic analysis technology has fully demonstrated the charm of microfluidic systems for manipulating microvolume fluids in microchannels. The droplet-based microfluidic analysis system uses immiscible liquid phases or gas phases as intervals between nL to pL-sized droplets to form independent reaction vessels, which have been successfully used in chemistry, physics, and biology. fields of science and medicine. Co...

AI Technical Summary

Problems solved by technology

However, this method requires manual preparation of the droplet loader, which is time-consuming and laborious, and requires the interface between the droplet loader and the chip to transfer the droplet into the chip.

In addition, under the pressure drive, especially in the microfluidic system using air bubbles to space the liquid section, and the mixing ratio of screening reagents and substrate reactants needs to be strictly controlled, which increases the difficulty of microfluidic manipulation.

Images