135 results about "Multiwell plate" patented technology

The utility model discloses a magnetic porous reaction plate, relates to a biological medicine research and development device, and is used for life science experiment research using magnetic beads. The reaction plate comprises a perforated plate, a base, a cover plate and a magnet, and the magnet can be embedded between the perforated plate and the base and is matched with the bottom of the perforated plate in shape. According to the utility model, the porous reaction plate can be moved, centrifuged, shaken and the like under the condition of keeping magnetic force, so that more flexible and diversified experiment designs and experiment schemes are realized.

The invention relates to a phenylacetylglutamine / phenylacetylglycine induced vascular smooth muscle cell senescence model and an establishment method and application thereof, and belongs to the technical field of cell models.The method comprises the following steps that vascular smooth muscle cell suspension is placed in a porous plate, 10% of FBS and 1% of mycillin are added into a culture medium, 24 h after cells adhere to the wall, the vascular smooth muscle cell senescence model is obtained, and the vascular smooth muscle cell senescence model is obtained. The method comprises the following steps of: adding a proper amount of phenylacetyl glutamine or phenylacetyl glycine dimethyl sulfoxide solution into a porous plate, changing a serum-free culture medium, continuously culturing for 24 hours, adding a proper amount of phenylacetyl glutamine or phenylacetyl glycine dimethyl sulfoxide solution into the porous plate, changing the culture medium added with phenylacetyl glutamine or phenylacetyl glycine once every 24 hours, and collecting cells after 72 hours. According to the invention, phenylacetylglutamine / phenylacetylglycine is used for inducing and establishing the vascular smooth muscle cell senescence model for the first time, the method is easy to operate and low in cost, and a foundation is laid for researching the relationship between cardiovascular diseases and senescence.

The invention discloses a flow intensity regulation and control device, a flow intensity regulation and control method and a laser proton accelerator. According to the device, a beam positioning sub-device is connected with a flow intensity adjusting and controlling sub-device, and the beam positioning sub-device is used for arranging the flow intensity adjusting and controlling sub-device in a vacuum cavity of the laser proton accelerator and adjusting the position of the flow intensity adjusting and controlling sub-device in the vacuum cavity; the flow intensity regulation and control sub-device comprises a movable perforated plate, a fixed perforated plate and a flow intensity regulation and control module; the movable perforated plate and the fixed perforated plate are overlapped and have the same shape, size and through hole arrangement; and the flow intensity regulation and control module is used for regulating and controlling the one-way relative displacement of the movable perforated plate and the fixed perforated plate so as to change the flow intensity regulation and control state of the flow intensity regulation and control sub-device on the laser proton beam. According to the embodiment of the invention, the beam flux can be rapidly and accurately adjusted, and the flow intensity of the proton beam can be rapidly and accurately adjusted.

The present invention concerns systems, materials, and methods of cell, cell organelle and / or particle separation and / or concentration utilizing magnetic levitation to affect separation. In some embodiments, the separation and / or concentration is performed in the wells of a multi-well plate.

1. The name of this design product: Multifunctional fluorescence analyzer. 2. Purpose of this design product: used for collecting fluorescence signals from multi-well plates. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture.

A device for CO2 microbubble flooding comprises a microbubble generating device, a stirring and liquid storage device, an injection pump, a gas compressor, a gas-liquid mixer and a pressure stabilizing system, wherein the microbubble generating device adopts an integrally formed multi-layer cylindrical metal powder-sintered porous plate, and the diameters of microchannels on the porous plate are sequentially reduced from inside to outside. A solution containing additives in the storage device is stirred uniformly and then enters into a downhole gas injection base pipe through the gas-liquid mixer to be injected into a stratum, then a carbon dioxide gas compressed by the gas compressor is pumped into the downhole gas injection base pipe through the gas injection pump, and the gas generates microbubbles in situ when passing through the multi-layer porous device under the action of pressure difference.

1. Name of the product in this design: Perforated plate (assembly). 2. Purpose of this design: A general-purpose dynamic joint component for assembling models. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

The present invention belongs to the technical field of mineral separation and processing, and discloses a multi-stage mineralization flotation column. It comprises a flotation column, a concentrate tank, an internal tank body, a raw material feed assembly and a middling ore circulation assembly. The interior of the flotation column is divided into a separation zone, a mineralization zone and a tailings zone from top to bottom; the mineralization zone is divided into a main mineralization zone and a middling ore zone by the internal tank body, and the top and bottom of the internal tank body are porous plates. The raw material feed assembly transports the mineralized slurry to the main mineralization zone through a vertically downward discharge port, and the middling ore circulation assembly sprays the incompletely mineralized middling ore back into the internal tank body through at least one group of middling ore feed pipes, forming a multi-stage coordinated mineralization zone. The present invention solves the problems of incomplete mineralization, poor bubble stability, and easy clogging of equipment in the prior art through multi-stage mineralization, thereby improving the mineral recovery rate and flotation efficiency.

Multi-well plate with a plurality of sample chambers (14), in particular arranged in a grid-like manner, wherein an inlet and an outlet chamber (16, 18) are each assigned to the sample chambers (14), wherein each sample chamber (14) is connected to the inlet chamber (16) assigned to it and to the outlet chamber (18) assigned to it in the lower region via connecting channels (20a, 20b), and with a cover part for the multi-well plate (10), wherein the cover part (12) covers the sample chambers (14) of the multi-well plate (10) towards the top, and wherein the cover part (12) has openings (24) assigned to the inlet chambers (16) of the multi-well plate (10), through which openings, when the cover part (12) is arranged on the multi-well plate (10), fluid can be supplied to the inlet chambers (16) from the outside.

This invention relates to the field of plant transgenic detection technology, and discloses a method for rapid batch detection of transgenic crops, comprising: Step 1, placing centrifuge tubes containing crop samples to be tested in an array into a multi-well plate to complete batch plate arrangement of samples; Step 2, performing overall mechanized processing based on the multi-well plate, so that the crop samples in the centrifuge tubes are simultaneously ground into powder under mechanical force. This integrated operation process, which places independent centrifuge tubes in an array within a multi-well plate and uses a tissue homogenizer, batch liquid addition equipment, and a centrifuge to simultaneously process all samples, achieves a significant improvement in detection throughput and efficiency. Compared to existing technologies that rely on manual, one-by-one operation, this method overcomes the shortcomings of low detection efficiency and the inability to simultaneously process multiple samples, thus failing to meet the needs of large-scale crop sample screening.

The utility model relates to an adjustable location fixture, including base and install the adjusting mechanism, perforated plate, adjustable location subassembly and adjustable clamping mechanism on the base, perforated plate is installed on the adjusting mechanism, adjustable location subassembly is movably installed on the perforated plate, adjustable clamping mechanism is installed on the adjustable location subassembly, and the adjustable clamping mechanism is installed on the base. The adjustable clamping assembly and the perforated plate are arranged adjacently, a plurality of mounting holes arranged in an array are formed in the perforated plate, the adjustable positioning assembly comprises a positioning rod and a plurality of positioning clamping blocks, and the positioning rod and the positioning clamping blocks can be connected with any mounting hole. The clamping device has the advantages of being capable of meeting the positioning requirements of different workpieces, good in clamping stability and capable of improving production efficiency.

The invention discloses a gas-liquid separator for an electrolytic hydrogen production system, the gas-liquid separator comprises an inlet pipe, a gas outlet, a liquid outlet, a liquid distributor and a multilayer plate, the liquid distributor is located at the tail end of the inlet pipe and comprises a perforated plate, and a gas-liquid mixture entering the gas-liquid separator is converted into a plurality of trickles; and the multi-layer plate is positioned below the liquid distributor, comprises multiple layers of separating plates which are arranged up and down, and is used for enabling the gas-liquid mixture output by the liquid distributor to enter the bottom of the gas-liquid separator after being subjected to gas-liquid separation of each layer of separating plate. The separation efficiency is improved through the multiple layers of separation plates, the effective separation area in the gas-liquid separator under the same volume is increased, the floating time needed by bubble separation is shortened, and the purpose of improving the separation efficiency is achieved through a simple structural internal part.

The present invention provides an apparatus for performing automated analysis of one or more samples in a single-well or multi-well plate or vial, wherein the automated analysis process includes automated fluid flow, wherein the sample comprises a fluid or particles in a sample vessel, and wherein the apparatus includes an assembly of components for processing the sample for analytical evaluation by a fluid-based and / or particle-based instrument. The automated fluid flow may include a system for moving the sample, including a vacuum system, a pressure-based system, a pneumatic system, a pump, a peristaltic pump, a diaphragm, or a syringe. The apparatus may include an assembly of components for achieving movement in the X, Y, and Z directions, as well as switches, microfluidic tubing, a well plate, an electronic pressure controller, a pneumatic or fluid mixing device, components for fluid processing, a sampling vessel, and mechanical components for mobilizing or transporting system components.

The invention relates to the technical field of experimental equipment, and provides an integrated desalting device and method for high-throughput sample treatment, and the device comprises a base, a movable partition plate and a standard porous plate. The movable partition has first and second working positions. And a wedge-shaped guide positioning mechanism is arranged between the movable partition plate and the perforated plate, and can automatically guide the movable partition plate to lift and transversely align through slope matching when the perforated plate is horizontally pushed in until the movable partition plate is stably supported on the perforated plate and accurate alignment of a sample treatment column and a collection hole is realized, so that rapid and accurate switching between a washing mode and an elution mode is completed. The device is ingenious in structure and easy and smooth to operate.

The utility model relates to the technical field of glue production, and disclose a kind of waste gas purification device for glue production, including purification bucket one and purification bucket two, the inside fixed of purification bucket one is equipped with filter screen, the middle part fixed mounting of pollution valve is positioned at the bottom of purification bucket one, the top of purification bucket one is fixedly equipped with bucket cover one, the inside fixed of bucket cover one is equipped with multi-orifice plate, the surface fixed of bucket cover one is equipped with pulse air pipe, the side fixed of purification bucket one is equipped with conduit;Through filter screen, waste gas can be pretreated, remove the impurities in waste gas, reduce the influence on activated carbon, through breathable support component, activated carbon can be loaded, and breathable capacity can be guaranteed, using the pore structure of activated carbon, waste gas can be adsorbed, by connecting pulse air pipe pulse fan, input pulse wind in bucket cover one, filter screen can be cleaned.

The utility model discloses a waste gas purification device for a chemical production workshop, which relates to the technical field of workshop air purification and comprises a base, a container fixedly mounted at the upper end of the base, an opening arranged at the upper end of the container, a first gas inlet pipe rotatably mounted at the bottom in the container and penetrating through the container, and a perforated plate fixedly mounted at the upper end in the container. According to the waste gas purification device for the chemical production workshop, the driving mechanism drives the movable pieces to rotate around the first gas inlet pipe, the movable pieces stir waste gas, the waste gas is evenly distributed at the lower end of the porous plate, the waste gas is evenly distributed on the porous plate, and therefore the waste gas is purified. The contact area between the waste gas and the porous solid adsorbent is increased, and the contact time is prolonged, so that the reaction efficiency between the waste gas and the purification medium is improved. Meanwhile, waste gas is prevented from forming dead angles in the container, and the residence time and accumulation of the waste gas are reduced.

The present invention discloses a multi-material breast patch TPU film processing device, including a conveying and winding mechanism, a cutting and collecting mechanism is provided on the top of the conveying and winding mechanism, the cutting and collecting mechanism includes a first concave block, two symmetrical hydraulic rods are installed on the outer wall of the first concave block near the top, the telescopic ends of the two hydraulic rods are installed with L-shaped limit blocks, a porous plate is fixed between the opposite sides of the two L-shaped limit blocks, the internal through holes of the porous plate are fixedly sleeved with circular column cutters, and the incision end of each of the circular column cutters is threadedly installed with a sealing plug, and a plurality of arc tubes are equidistantly distributed and fixedly penetrated on one side of the first concave block. By arranging the cutting and collecting mechanism, the present invention can effectively improve the complete collection of the circular TPU film after the TPU film is die-cut, that is, effectively improve the utilization efficiency of the TPU film processing device.

The invention provides a single cell ATAC sequencing library construction method and a kit, the construction method comprises the following steps: (1) subpackaging a cell sample into a porous plate, adding a mixed solution containing a first transposon and a second transposon into each pore, and co-incubating to obtain a cell containing fragmented DNA; (2) carrying out splitting decomposition on the cells containing the fragmented DNA, and retaining the fragmented DNA; and (3) amplifying the fragmented DNA to obtain the sequencing library. The construction method is high in transposition efficiency, not limited by a microfluidic or microwell plate platform, appropriate in label combination information amount, low in requirements for the cell number, the cell type and the tissue state, suitable for a conventional double-end sequencing platform, high in sequencing data quality and high in application value.

The utility model relates to the technical field of experimental instruments, and discloses a clinical medical examination oscillation device which comprises an oscillation device body, an oscillation plate is installed in the oscillation device body, the periphery of the top of the oscillation device body is fixedly connected with a sleeve shell, sliding grooves are formed in the two sides of the sleeve shell, and the top of the sleeve shell is fixedly connected with the oscillation plate. A containing plate is slidably connected to the inner wall of the sliding groove, shells are fixedly connected to the two ends of the top of the containing plate, inserting rods are slidably connected to the inner walls of the shells, first springs are fixedly connected to one sides of the inserting rods, the other ends of the first springs are fixedly connected with the shells, and porous plates are fixedly connected to the two sides of the sleeve shell. According to the clinical medical examination oscillation device, the stress amplitude of a test tube in the oscillation process is changed by adjusting the height of the containing plate, the oscillation effect of different frequencies and amplitudes is achieved, and the different requirements of clinical medical examination sample treatment with different oscillation requirements of the same batch are met.

The utility model relates to a ceramic cylindrical membrane sealing structure for beer filtration, which belongs to the technical field of beer filtration and comprises a cylinder body, and a plurality of cylindrical ceramic membranes are arranged in the cylinder body. The utility model has the beneficial effects that the high-temperature-resistant rubber pad is arranged between the upper flange and the barrel flange, so that a first sealing layer can be formed, the high-temperature-resistant rubber pad has the main functions of sealing and protection, and the high-temperature-resistant rubber pad can be kept stable under extreme conditions; the problem that a sealing material is prone to failure under the working conditions of high temperature and high pressure is effectively solved, a sealing ring EDPM in the sealing assembly can be pressed through the effect of a pressing plate, a second sealing layer is formed by combining the sealing performance of the sealing ring EDPM, a sealing matching face used in cooperation with the sealing ring EDPM is machined above a porous plate, a third sealing layer can be formed, and therefore the sealing effect of the sealing assembly is improved. And a multi-layer sealing structure is adopted, so that the leakage risk caused by non-uniform sealing pressure can be avoided.

An apparatus, multi-well plate and method for automated cell lysis and nucleic acid purification and amplification. The plate includes a lysis well, at least one wash well, an elution well, and a PCR chip. The apparatus includes a vertically aligned rotor mixer comprising a magnetic tip and actuators for moving the rotor mixer in a vertical and horizontal directions, to transfer magnetic beads from well to well. The rotor mixer is used to vortex lysis mixtures, wherein the vortexing speed is sufficient to overcome the magnetic attraction between the beads and mixer tip and disperse the beads in solution, to collect nucleic acids such as DNA in an elution solution that is transferred to the PCR chip for amplification of target sequences.

The invention belongs to the technical field of detection, and particularly relates to an optical device for pore plate fluorescent material detection, which comprises a tunable laser light source, a reflective optical fiber collimator, an optical filter assembly, a beam splitting cube, a beam expanding lens group and a microlens array, the beam splitter is used for splitting the expanded light beams and focusing the light beams to all the sample holes at the same time to realize parallel excitation. Parallel excitation and detection are achieved through the microlens array, chromatic aberration is eliminated through the reflective light path design, the problems that a traditional hole-by-hole detection mode is low in efficiency and prone to causing fluorescence quenching and protein inactivation are effectively solved, the detection efficiency and precision are remarkably improved, and the detection cost is reduced. The method has a wide application prospect in the fields of biomedical research and clinical diagnosis.

The utility model discloses a perforated plate inoculation instrument. The porous plate inoculation instrument comprises a rotary uniform mixing sample tube mechanism and an alternate uncovering mechanism, wherein the rotary uniform mixing sample tube mechanism comprises a fixed base plate, a support frame, a rotary fixed frame, a rotating wheel, a driving structure and a sample tube frame. According to the perforated plate inoculation instrument provided by the embodiment of the invention, the full-process automatic operation is realized by arranging the rotary uniform mixing sample tube mechanism and the alternate uncovering mechanism, and the rotary uniform mixing sample tube mechanism comprises the fixed base plate, the supporting frame, the rotary fixed frame, the rotating wheel, the driving structure and the sample tube frame; the driving structure can be used for driving the rotating wheel to rotate so that the fixing plate can turn over the sample tube frame, the rotating speed of the sample tube frame is increased, the uniform mixing treatment efficiency of sample tubes arranged on the sample tube frame is improved, the cover opening and liquid pumping speed is increased in cooperation with the alternate cover opening mechanism, and the inoculation efficiency is improved.

A main body part of the gas-liquid parallel flow type internal circulation reaction equipment is of a flat rectangular structure and sequentially comprises a ceramic membrane assembly, a perforated plate gas inlet cavity, a perforated plate with a flange structure, a reactor feed port, a reactor main body part, a reactor discharge port and an overflow structure from bottom to top, the two built-in guide plates divide the loop reactor into an ascending area, a descending area, a bottom gap area and a gas-liquid separation area; a screen is mounted in the middle of the rising area guide plate; according to the reactor, a multi-channel ceramic membrane assembly is arranged in the center of an air inlet plate to generate small-size bubbles, and a porous plate structure is adopted to generate large-size bubbles; the porous plate air inlet holes are distributed on two sides of the ceramic membrane air inlet. According to the invention, millimeter-level bubbles and micron-level bubbles are simultaneously fed, the difference of turbulence degrees at different radial areas is reduced, and the mass transfer efficiency and the adaptive capacity under different working conditions are improved. The energy consumption is reduced, and the manufacturing and production cost is reduced.

The invention provides inter alia a method for the production of an adherent cortical organoid, comprising the steps of: (a) providing a cell culture comprising a population of neural progenitor cells (NPCs); (b) seeding neural progenitor cells (NPCs) of said population in a well of a multiwell plate; wherein said well comprises a bottom surface (106) and an upright wall (104), and wherein a distance (x) between a centre (112) of the bottom surface and the upright wall is between 1.1 mm and 1.9 mm; and (c) culturing said seeded NPCs in a culture medium for neural differentiation for a period of time, to thereby allow formation of an adherent cortical organoid.

The invention discloses a construction method of a model for respiratory tract infection caused by inhalation of pathogenic microorganism aerosol, which comprises the following steps: inoculating respiratory tract cells to an upper chamber of a Transwell cell, culturing until a complete cell barrier is formed, and transferring the cell-loaded cell to an exposure cavity; atomizing the pathogenic microorganism suspension into aerosol, and regulating and controlling environmental parameters of the aerosol in real time; introducing the aerosol into an exposure cavity, and enabling the aerosol to be in direct contact with cells in a manner of simulating the inhalation process of the respiratory tract of a human body; and after the exposure is finished, transferring to a porous plate for continuous culture. The invention further discloses application of the model for respiratory tract infection caused by inhalation of the pathogenic microorganism aerosol. The method has the beneficial effects that the model construction process is simple and convenient, the natural infection process can be highly simulated, the method can be used for simulating a bioaerosol exposure scene after specific treatment, and the method is suitable for pathogen invasion mechanism analysis, host immune response research and efficient screening of anti-infection drugs.

The present invention relates to an electrically conductive pipette mounting adapter, a sample tube opening and closing device, and a sample automatic analysis system, and more particularly to an electrically conductive pipette mounting adapter, a sample tube opening and closing device, and a sample automatic analysis system that integrally perform dispensing of a liquid sample, extraction of nucleic acid, amplification, and detection. A sample tube opening and closing device includes a housing (110) forming an internal space isolated from the outside, including a door portion (120) for transporting a multi-well plate (20) for biological samples having a plurality of sample tubes (10) for containing biological samples into and out of the internal space; and a sample tube opening and closing portion provided in the internal space apart from the multi-well plate (20) for biological samples, automatically opening and closing the sample tubes (10).

Methods of generating organoids on multi-well plates are provided by depositing a polymeric solution comprising cells under conditions which result in a homogenous population of organoids, which can be used for high throughput analysis for drug screening and for determining treatment regimens of a drug.

The present invention relates to an apparatus for demagnetizing magnetized magnetic particles. The apparatus demagnetizes and disperses magnetic particles to increase the probability of detecting the magnetic particles. The apparatus includes (a) multi-well plate with single or multiple wells arranged, (b) magnetic particles dispensed into the multi-well plate, and (c) a support means supporting the multi-well plate. A demagnetizer capable of moving up and down is provided below the support means. The support means includes a seesaw-type shake

A multi-well plate for cell culture growth having a reduced maximum depth is disclosed. The multi-well plate may further comprise a reduced skirt height.