114 results about "Strychnine" patented technology

The invention relates to an action of ferulic acid on enhancing the drug effect of some medicaments and a purpose thereof, wherein the medicaments mainly comprise alkaloid medicaments including matrine, kushenin, sophocarpine, hyoscyamine, narceine, hanfangchin A, jamaicin, morphine, codeine, evodiamine, strychnine, catharanthine, vincristine, taxol, verticine, peimine, peiminine, ephedrine, pseudoephedrine, wilfordine, triptolide, tripdiolide and the like, and flavonoid medicaments including puerarin, ginsenoside, ginsengenin, mangiferin, scutelloside, alkannin, meletin, rutin, hesperidin, daidzin, soybean isoflavone, daidzein, carthamin, catechin and the like. The ferulic acid and the medicaments can form a compound or a medicament compound, or the ferulic acid and the medicaments can generate a chemical reaction (including salification, esterification, amidation, ketonization, etherification and the like), and / or the ferulic acid and the medicaments can generate a synergistic effect and an additive effect.

The invention discloses a nanometer vauqueline liposome and preparation method which consists of dissolving strychnine, lecithin and cholestrin into organic solvent with a predetermined volume, removing organic solvent from the solution through depressed evaporation for film forming, charging microcosmic salt cushioning solution containing a predetermined concentration of surface active agent, and carrying out swelling, free assembling, ultrasonic dispersing. The prepared strychnine liposome suspending liquid can be used for treating various diseases including gout, arthritis and traumatic injury.

A quality control method for the Chinese medicine to treat thrombus features that a thin-layer chromatography is used for the identification to earthworm, vomiting nut, musk, corydalis tuber, toad, liquorice root, etc and an efficient liquid-phase chromatography is used to measure the contents of tanshinone IIA and strychnine.

The present invention discloses a method for extracting total alkaloids in semen strychni, and belongs to the technical field of drugs. The method comprises the steps of extracting semen strychni crude powder with acid, filling on a column, separating, and purifying to remove some of strychnine. The prepared semen strychni total alkaloid content is above 50%, wherein the content ratio of alkaloids and strychnine is about 2:1, thereby effectively reducing the toxicity and improving the safety of clinical medication. The extraction method can efficiently separated high purity semen strychni total alkaloids from the traditional Chinese medicine of semen strychni.

The invention discloses a method for obtaining purified total alkaloid of strychnos nux-vomica by removing partial strychnine in the total alkaloid of the strychnos nux-vomica, which comprises the following steps: extracting with a certain concentration of ethanol, dissolving the extract with hydrochloric acid ; adjusting the pH value to be alkaline after dissolving; carrying out extracting and pressure reduction on dichloromethane to remove solvent to obtain the total alkaloid of the strychnos nux-vomica; ultrasound dissolving total alkaloid powder of strychnos nux-vomica with ethanol; carrying out pressure reduction and concentration to a certain volume; centrifuging the concentrated solution, taking supernate, refrigerating and standing to remove precipitate; and drying the supernate to obtain the purified total alkaloid of strychnos nux-vomica. The content of strychnine in the total alkaloid of strychnos nux-vomica extracted by the method is remarkably reduced; and in addition, the method has safe extraction process, low cost and is beneficial to industrial scale production. Proved by the evaluation result of a pharmacological test, the effects of analgesia, anti-inflammation, immunity and antitumor activity of the purified total alkaloid of strychnos nux-vomica are remarkably superior to the effects of total alkaloid of strychnos nux-vomica and the toxicity is remarkably reduced.

The invention discloses a nanometer vauqueline liposome transdermal spray agent, its preparation method and application, wherein the preparation is prepared through mixing nano strychnine liposome freeze-drying powder containing a predetermined of transdermal promoting agent and freeze-drying protecting agent with physiological saline by a predetermined proportion. The prepared preparation is a nano liposome with dispersed constituents and good stability. It can be used for the treatment for various diseases including gout, arthritis and traumatic injury.

The invention discloses a strychnine compound phospholipid liposome which is prepared by two types of phospholipids, strychnine and cholesterol in certain weight ratio. The preparation method for the strychnine compound phospholipid liposome comprises the following steps: (1) dissolving the first type phospholipid, the second class phospholipid and the cholesterol in absolute ethyl alcohol, and injecting the aqueous solution of ammonia sulfate into the mixture ; (2) removing the alcohol through heating or decompression method; (3) carrying out ultrasonic processing or high-pressure emulsion homogenization to reduce the grain size of the liposome; (4) removing the ammonia sulphate which is not encapsulated by the liposome through a dialysis method, an ultrafiltration method or a gel column chromatographic method so as to form blank liposome suspension; and (5) adding the strychnine into the suspension, and carrying out moderate incubation so as to enable the strychnine to be encapsulated by the liposome. The strychnine compound phospholipid liposome has significant characteristics of high encapsulating rate of the liposome for the strychnine, greatly improved stability, no easy leakage, and remarkably improved medicine carrying capacity. The results of tumor resisting experiments show that the cancer resisting effect of the strychnine compound phospholipid liposome is remarkably higher than that of a common single phospholipid liposome.

The invention provides Chinese medicinal nux vomica capsules for treating myasthenia gravis, which is characterized in that: processing procedures of water soaking, unhairing, frying and the like are adopted, so that the toxicity of a main active ingredient, namely strychnine is obviously reduced. The preparation process is strict and standard, easy to operate, and controllable in quality and canensure the quality stability and clinical administration safety of the nux vomica capsules. Through years of clinical observation, the capsules have satisfying curative effects and small toxic and side effects, can control slight myasthenia gravis through a single medicine, can treat severe myasthenia gravis cooperatively, can obviously reduce the using amount of western medicines and the relatedtoxic and side effects, and improve the curative effects, so the capsules have good development and clinical application prospect.

The invention discloses an antidotal capsule of a Tibetan medicine compound and a quality detection method of a preparation of the antidotal capsule. Content of strychnine of semen strychni is controlled by a high performance liquid chromatography (HPLC) process; iron content in iron powder is controlled by means of titration; cholic acid in calculus bovis artifactus and hydroxy saffionin A in safflower are detected quantitatively by the high performance liquid liquid-phase chromatography; so that efficacy is guaranteed while hidden dangers to health of patients when the patients take excessive antidotal capsules are avoided, and product quality is improved, product safety, stability and quality controllability are guaranteed.

The invention discloses an extraction, separation and purification method for strychnos total alkaloid, and belongs to the technical field of medicines. The method comprises but is not limited to the following steps of: treating strychnos coarse powder via an acid water extraction-organic solvent extraction method or an alkalization-organic solvent extraction method or an alcohol extraction-organic solvent extraction method to obtain the strychnos total alkaloid; purifying the total alkaloid via a solvent-stepwise extraction method or a column chromatography method or a hydrochloride-sulphate stepwise crystallization method; and then preparing via the hydrochloride-sulphate stepwise crystallization method or the column chromatography method to obtain strychnine having a purity of greater than 90%.

The invention relates to the technical field of traditional Chinese medicines, in particular to a construction method of an HPLC characteristic chromatogram of Jingyaokang capsules. The construction method comprises the steps that a test solution is prepared, wherein the content of the Jingyaokang capsules is extracted with a methanolic hydrochloride solution, and a neutral alumina column is addedto obtain the test solution; strychnine serves as a reference substance; the test solution and a reference substance solution are detected by adopting high performance liquid chromatography separately to obtain the HPLC characteristic chromatogram of the Jingyaokang capsules, wherein according to conditions of high performance liquid chromatography, a C18 column serves as a chromatographic column, a mobile phase A is acetonitrile, a mobile phase B is a mixed solution of water, acetic acid and triethylamine, and gradient elution is carried out. The similarity between the HPLC characteristic chromatogram, constructed through the method, of the Jingyaokang capsules and a reference chromatogram is greater than 0.90, the quality of the Jingyaokang capsules can be effectively represented, and the product quality can be comprehensively monitored. The method has the advantages of being convenient to use, quick, high in precision, good in reproducibility and the like.

A pharmaceutical composition comprising a herbal extract, comprising a mixture of saponins prepared from the pericarp of Sapindus trifoliatus, with binding affinities for the receptor sites viz. GABA-A agonist site, Glutamate-AMPA site, Glutamate-Kainate site, Glutamate-NMDA agonistic site, Glutamate-NMDA glycine (strychnine insensitive) site and Sodium channel (site 2), having major mediatory role in anticonvulsant activity. A process for preparation of the herbal extract; isolation of six pure compounds from the mixture of saponins in the aqueous extract; and a pharmaceutical composition comprising the said extract in combination with pharmaceutically acceptable additives. A method of prophylactic treatment of migraine through anticonvulsant activity of the composition by its administration through intranasal route.

The invention discloses a hydroxypropyl-beta-cyclodextrin inclusion of strychnine and a preparation method thereof. The inclusion is prepared from the strychnine and hydroxypropyl-beta-cyclodextrin in weight ratio of 1: (1-100) by using the preferential inclusion process. Experimental results show that the hydroxypropyl-beta-cyclodextrin inclusion of the strychnine has the advantages of high inclusion rate, great drug loading and good stability, and can significantly improve the water solubility of the strychnine, lead the solubility in water to be more than 20 times that of the strychnine, significantly improve the bioavailability after administration of the strychnine, play a role in reducing the toxicity of the strychnine and lead the clinical application to be safer.

The invention relates to the field of medicine, and discloses composition for a 3D printed cartilage bracket, a microsphere containing the composition and a preparation method of the composition. The composition comprises polylactic-co-glycolic acid, brucine, polyvinyl alcohol, chitosan and optional strychnine. Taking the total weight of the composition as the baseline, the total content of brucine and optional strychnine is 0.1-10 wt%. The composition for the 3D printed cartilage bracket can be taken as raw materials of the 3D printed cartilage bracket and has appropriate degradation speed; degradation products are harmless to human bodies, good biocompatibility is achieved, adhesion and proliferation of cells are facilitated, and cartilage tissue is promoted to form; besides, a slow-release medicine function is achieved, and the composition has no adverse influence on efficacy.

The present invention relates to the discovery that specific human taste receptors in the T2R taste receptor family respond to particular bitter ligands, i.e., acetaminophen, ranitidine, strychnine and denatonium. The present invention further relates to the use of these receptors in assays for identifying ligands that modulate the activation of these taste receptors and which may be used as additives in foods, beverages and medicinals for modifying (blocking) T2R-associated bitter taste.

The invention discloses a method for extracting, separating and purifying strychnine, and belongs to the technical field of medicine. The method includes but is not limited to the steps of: taking strychnos nux-vomica L., grinding the taken strychnos nux-vomica L. into coarse powder, performing extraction by using ethanol, combining extract, performing filtration, concentrating filtrate, and recovering the ethanol until no alcohol is smelt so as to obtain concentrated extract of total alkali of strychnos nux-vomica L.; acidifying the concentrated extract of total alkali of strychnos nux-vomicaL., performing extraction for impurity removal by adding an organic solvent, performing alkalinization on raffinate, and performing extraction by adding an organic solvent so as to obtain extract; adding the extract into a silica gel chromatographic column for elution, collecting the strychnine section of eluent, performing combining, concentration and drying, and performing purification by usinga recrystallization method to obtain the strychnine with a purity of 95% or more.

The invention belongs to the field of pharmaceutical preparations, and particularly relates to a strychnine gel preparation and a preparation method thereof. The gel preparation comprises 0.5%-1% of strychnine, 0.5%-3% of a gel skeleton, 10%-30% of a cosolvent and the balance of water. According to the prepared strychnine hydrogel, simple pharmaceutic adjuvants are used, no transdermal enhancer isused, but the transdermal effect is excellent, and the strychnine hydrogel is easy to spread, good in biocompatibility, good in skin absorption, good in drug membrane adhesiveness and free of irritation to skin and mucous membranes.

The invention provides a strychnine molecular imprinting polymer and its preparation method, the method comprises the following steps: dissolving the functional monomers and the template molecule strychnine in dimethyl sulfoxide, mixing for 6-24 hours at room temperature, then adding a cross-linking agent ethylene dimethacrylate and an initiator, performing ultrasonic degassing, introducing nitrogen for deoxygenation, sealing and carrying out thermal-initiated polymerization, cleaning and removing the template molecule, wherein the functional monomers contain allyl-beta-cyclodextrin and methacrylic acid. According to the invention, the molecular recognition capability of the beta-cyclodextrin and the molecular recognition capability of the molecular imprinting technology are used for preparing the strychnine molecular imprinting polymer, the proportion of various raw materials are optimized to obtain good recognition effect. The modification of the beta-cyclodextrin has large influence on its molecular recognition effect, therefore allyl which possesses small structure modification to the beta-cyclodextrin can be selected and introduced to a polymerizable unit, and dual recognition capability of the beta-cyclodextrin and the molecular imprinting technology can be fully used to increase the recognition capability. The bifunctional monomers and a plurality of recognition modes are used for raising the recognition capability further.

The invention relates to a preparation method of strychnine immune nanoparticles, and the preparation method comprises the following specific steps: step 1, dissolving carboxylated poly(ethylene glycol)-polylactic acid block copolymer and strychnine in an organic solvent, and then mixing with a polyvinyl alcohol aqueous solution to form a primary emulsion; step 2, removing the organic solvent andimpurities to obtain a strychnine nanoparticle concentrate; and step 3, sequentially adding a carbodiimide salt used as a carboxyl activation reagent and an anti-human-AFP (alpha-fetoprotein) monoclonal antibody to the strychnine nanoparticle concentrate, thus linking the carboxyl group on polyethylene glycol and the amino group of the anti-human-AFP monoclonal antibody by chemical coupling to obtain the strychnine immune nanoparticles. The preparation method provided by the invention has the advantages of simple process, high encapsulation rate, stable drug release and good drug ballability;and the obtained strychnine immune nanoparticles are used for preparing anti-tumor immune targeting drugs, and have the advantages of precise targeting drug accumulation in tumor tissue cells, stabledrug release, good anti-cancer effect, safety and the like.

The invention discloses a preparation process for separating and purifying strychnine from total alkali of nux vomica. Pure strychnine with the content exceeding 94% is prepared by taking total alkali of nux vomica as the raw material through a column chromatography and organic solvent extraction purification and separation method. The pure strychnine can be used as a chemical standard substance and a reference substance of nux vomica or an extract of the nux vomica or used as a raw material of a preparation. The preparation process is not only low in cost, simple and easy in process, but also applicable to the preparation of a larger amount of pure strychnine, and has wide social and economic effects.

The invention discloses a quality control method for orthopedics-traumatology coaptation. The method adopts TLC to carry out qualitative identification for notoginseng, borneol, safflower and Nux vomica powder and uses electrophoresis for carrying out qualitative identification for starfish. HPLC and GC are respectively adopted to carry out quantitative identification for strychnine and the borneol. Chemical titration is adopted to carry out quantitative identification for mercury sulfide. Therefore, the quality control scope of the orthopedics-traumatology coaptation is enlarged. The quality control method for orthopedics-traumatology coaptation is easy to be practiced and has strong specialization. In addition, the quantitative identification has high precision and good repeatability, thus ensuring that the quality of compound medicine is uniform, stable, effective and controllable.

The invention discloses a straw biomass charcoal for soil conditioner modified by adding 3-indolebutyric acid. The straw biomass charcoal is prepared from, by weight, 83-85 parts of wheat straw, 14-16 parts of folium artemisiae argyi, 3.6-3.8 parts of calcium chloride, 1.1-1.5 parts of potassium chloride, 1.5-1.8 parts of citric acid, 2-4 parts of powdery silicon fertilizer, 0.2-0.5 part of gamma-amino butyric acid, 4-6 parts of brown algal powder,1-3 parts of tea meal, 0.03-0.05 part of strychnine, 0.6-0.9 part of 3-indolebutyric acid, 6-7 parts of bentonite, a proper amount of 10% sodium hydroxide solution by weight, a proper amount of mixed acid liquid and a proper amount of deionized water. The straw biomass charcoal prepared by soaking the wheat straw and the folium artemisiae argyi with the mixed activator obtained by compounding the sodium hydroxide solution, the calcium chloride and potassium chloride, conducting high-temperature carbonization and pickling treatment on the mixture and then compounding 3-indolinic acid, the tea meal and other components has the effects of rooting and killing insects, the comprehensive utilization rate of crop straw is improved, and the yield and quality of agricultural products are also improved.

The invention discloses a processing method of traditional Chinese medicine semen strychni. The processing method of the traditional Chinese medicine semen strychni comprises the steps that the traditional Chinese medicine semen strychni is placed in water to be subjected to dip bleaching; the traditional Chinese medicine semen strychni after being subjected to dip bleaching is subjected to peeling, vinegar-adding and joint-boiling, and drying is conducted; and the traditional Chinese medicine semen strychni after being dried is sand-fried, and a semen strychni processed product is obtained after sand frying is conducted. According to the processing method of the traditional Chinese medicine semen strychni, the content of strychnine and strychnine in the processed semen strychni can be effectively reduced, and the content of the strychnine with great toxicity can be effectively reduced to an effective range for safe using. The processing method of the semen strychni adopts a method of vinegar soaking and sand scalding which is different from a traditional sand scalding method, the content of the toxic components of the strychnine and the strychnine can be controlled in a safe range, and the clinical effects of activating blood, dredging collaterals, relieving pain, resolving hard lumps and reducing swelling can be achieved. Thus, the processing method can achieve a good effect of reducing poisonousness and stabilizing the effect on the traditional Chinese medicine semen strychni, and the processing method of the traditional Chinese medicine semen strychni is more effective. The processing method is controllable, stable and reliable.

The invention discloses a drug combination with inflammation diminishing and cough relieving functions. Health food is prepared from the following raw materials in parts by weight: 14-22 parts of nux vomica, 6-14 parts of Sapindus mukorossi Gaertn and 4-8 parts of oriental wormwood. In the components, the nux vomica serves as a monarch drug, has a main ingredient of strychnine, can cause nerve excitement, has a paralysis function on a nerve caused by cough, and achieves a cough relieving effect; the Sapindus mukorossi Gaertn serves as a ministerial drug and has the effects of diminishing inflammation and sterilizing, and promote the secretion of saliva or body fluid to moist the throat; the oriental wormwood serves as an adjuvant and has the effects of dispelling wind and clearing heat, and tonifying deficiency and relieving a cough.

The invention relates to a procedure for screening of neuroactive substance and the associated neural plasticity by treating fruitfly Drosophila melanogaster adult males with fly medium containing either of the neuroactive compounds strychnine, pentylenetetrazol, pilocarpine hydrochloride, tetraethylammonium chloride, lithium carbonate and ethosuximide, subjecting flies to negative geotaxis and horizontal locomotor assays, observing the height climbed and distance walked by and the climbing speed of the flies wherein an altered height climbed by flies under drug treatment and a long-lasting alteration in climbing speed of flies after drug withdrawal is most characteristic of the neuractive compounds.

The invention discloses a high-temperature colored penetrating flaw detection agent, which is prepared from the following raw materials in parts by weight: 35-60 parts of diisooctyl phthalate, 6-12 parts of polyoxyethylene fatty acid ester, 25-25 parts of stryne 40 parts, 5-20 parts by weight of surfactant, 1-4 parts of selenium nitrogen red and 0.7-1.2 parts of propellant. The invention provides a high-temperature colored penetrant flaw detection agent, which can meet the penetration detection at a high temperature of 150° C., without the need for equipment to stop production for detection, and greatly improves the detection efficiency and accuracy.

The present invention discloses a strychnine-hydroxypropyl-beta-cyclodextrin liposome, which contains, by weight, 0.5-2 parts of strychnine, 2-20 parts of a strychnine-HP-beta-cyclodextrin inclusion compound, 20-200 parts of soybean lecithin, and 5-20 parts of cholesterol. The present invention further discloses a strychnine-hydroxypropyl-beta-cyclodextrin liposome preparation method, which comprises: carrying out inclusion on strychnine with hydroxypropyl-beta-cyclodextrin, and then preparing into the liposome. According to the present invention, drug loading capacity is improved, an elimination half-life of the strychnine is prolonged, stability is improved, lymphatic system targeting is achieved, toxic and side effects on heart and central nervous system are reduced, and bioavailability is improved.

The invention discloses a process for surface inspection of precise metal parts through a penetration method. The process comprises the following steps: (1) cleaning; (2) applying penetrating agents,preparing two penetrating agents, putting the metal parts in an environment of 60-70 DEG C, standing for 1-3 minutes, coating defected parts on the surfaces of the metal parts with the first penetrating agent, putting the metal parts in an environment of 15-20 DEG C, standing for 8-10 minutes, and spraying the second penetrating agent to the defected parts on the surfaces of the metal parts, wherein the first penetrating agent contains diisooctyl phthalate, strychnine, silicon carbide, an activating agent and phthalate, and the second penetrating agent contains bisazo red, ethanol, glycol ether and rose red; (3) cleaning the redundant penetrating agent; (4) carrying out development; and (5) examining. The process disclosed by the invention has the beneficial effects that the steps are simple, the operation is convenient and rapid, a result is visual, the observation is convenient, a professional inspection worker is not required, and the operation can be carried out by a common procedure worker after simple training.

The invention discloses a limited inspection method for strychnine in Huatuo reconstituted pills, comprising: 1) taking Huatuo reconstituted pills, weighing the powder, adding extraction solvent, ethanol, ammonia water, ultrasonic treatment; filtering, evaporating the filtrate to dryness, Add acid solution to the residue, heat to dissolve, adjust pH value, shake and extract with organic solvent, evaporate to dryness, add chloroform to dissolve the residue, and use it as the test solution; 2) take strychnine reference substance, add chloroform Make a solution as the reference substance solution; 3) draw the test solution and the reference substance solution, respectively point on the same silica gel G thin-layer plate, develop with a developing agent, take it out, dry it, spray it with a chromogenic agent, and use it for testing. In the chromatogram of the reference substance, there should be no spots at the position corresponding to the chromatogram of the reference substance, or the spots should be smaller than those of the reference substance. The invention can well control the quality of Huatuo Zaizao Pills, ensure the stable, uniform and controllable quality of the pills, and ensure the effectiveness and safety of human medicine.

The present invention is high efficiency liquid phase-ultraviolet detector process of determining the content of strychnine as the main active component in refined Haimazhuifeng plaster. The process includes the main steps of preparing test sample solution, preparing the contrast solution, and determining. Compared with available thin layer process, the high efficiency liquid phase-ultraviolet detector process of the present invention has the features of high sensitivity, small measurement error, high separating degree, high stability, high repeatability, simple operation, etc.