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Method for detecting content of strychnine in refined Haima Zhuifeng plaster

A technology of strychnine and a determination method, which is applied in the field of determination of strychnine content of Ninghe, can solve the problems of poor reproducibility, large influence of environment and operation, large detection error of thin-layer chromatography, etc. Small error, guaranteed safety and effectiveness, low environmental and operational impact

Active Publication Date: 2007-04-11
黄石卫生材料药业有限公司
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0005] The national standard for the determination of the content of refined Haima Zhuifeng cream is to use thin-layer chromatography to determine the content of strychnine in Nuxyca chinensis. However, due to the large detection error and poor reproducibility of thin-layer chromatography, it is affected by the environment and operation. Great influence and other limitations have been gradually eliminated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Determination according to high performance liquid chromatography (Chinese Pharmacopoeia 2005 edition appendix VID).

[0026] Chromatographic conditions and system suitability test: use Diamonsil C18 column (4.6×250mm, 5μm); mobile phase is acetonitrile-methanol-0.05mol / L potassium dihydrogen phosphate solution (containing 0.001mol / l sodium dodecyl sulfate) - Triethylamine (30:15:54:1), adjust the pH to 3.5 with phosphoric acid; the detection wavelength is 254nm; the flow rate is 1ml / min; column temperature: room temperature. Injection volume: 20ul; retention time: strychnine about 10min, strychnine about 13min. The detection limits of strychnine and strychnine using this liquid phase method are respectively 0.5ng and 0.6ng; Strychnine showed a good linear relationship (r=0.9999) in the range of 16-186 μg / ml; both solutions were stable within 8 hours; precision of strychnine: RSD=0.64%; precision of strychnine: RSD= 0.81%, indicating that the method has good precision...

Embodiment 2

[0032] Determination according to high performance liquid chromatography (Chinese Pharmacopoeia 2005 edition appendix VID).

[0033] Chromatographic conditions and system suitability test: use Diamonsil C18 column (4.6×250mm, 5μm); mobile phase is acetonitrile-methanol-0.002mol / l sodium dodecyl sulfate aqueous solution-glacial acetic acid (35:45:20:0.5) ; Detection wavelength is 254nm; Flow rate is 1ml / min; Column temperature: room temperature. Injection volume: 20ul; retention time: about 6 minutes for strychnine, about 9 minutes for strychnine. The detection limits of strychnine and strychnine were respectively 0.5ng and 0.6ng by this liquid phase method; Good linear relationship (r=0.9995) in the range of 10-100 μg / ml; both solutions are stable within 8 hours; precision of strychnine: RSD=1.15%; precision of strychnine: RSD=1.12 %, indicating that the method has good precision.

[0034] Preparation of the test solution: take 100cm of refined hippocampus Zhuifeng ointment...

Embodiment 3

[0039] Determination according to high performance liquid chromatography (Chinese Pharmacopoeia 2005 edition appendix VID).

[0040] Chromatographic conditions and system suitability test: use Sinochrom ODS-BP (Dalian Elite Company) C18 column (4.6×250mm, 5μm); mobile phase is methanol-0.001mol / l sodium dodecyl sulfate aqueous solution-glacial acetic acid-three Ethylamine (52:48:0.5:0.135); detection wavelength: 254nm; flow rate: 1ml / min; column temperature: 40°C. Injection volume: 20ul; retention time: strychnine about 12min, strychnine about 14min. The detection limits of strychnine and strychnine using this liquid phase method are respectively 0.6ng and 0.8ng; Strychnine showed a good linear relationship (r=0.9998) in the range of 8.8~88 μg / ml; two kinds of solutions were all stable within 8 hours; precision of strychnine: RSD=0.55%; precision of strychnine: RSD= 0.14%, indicating that the method has good precision.

[0041] Preparation of the test solution: take 200cm of ...

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Abstract

The present invention is high efficiency liquid phase-ultraviolet detector process of determining the content of strychnine as the main active component in refined Haimazhuifeng plaster. The process includes the main steps of preparing test sample solution, preparing the contrast solution, and determining. Compared with available thin layer process, the high efficiency liquid phase-ultraviolet detector process of the present invention has the features of high sensitivity, small measurement error, high separating degree, high stability, high repeatability, simple operation, etc.

Description

technical field [0001] The invention relates to a quality detection method of refined Haima Zhuifeng ointment, in particular to a method for determining the contents of strychnine and strychnine as main active ingredients in the refined Haima Zhuifeng ointment. Background technique [0002] Refined Haima Zhuifeng Plaster is a new type of transdermal plaster. It is refined from more than 20 kinds of precious Chinese medicinal materials such as hippocampus, angelica, Achyranthes bidentata, Chuanxiong, and Eucommia. It has the functions of dispelling wind and cold, promoting blood circulation and relieving pain. It has significant curative effect on wind-cold, numbness, waist and leg pain. [0003] Refined hippocampus Zhuifeng ointment contains strychnium seed, achyranthes bidentata, windproof, eucommia, cinnamon, safflower, red peony root, gastrodia elata, frankincense, borneol, angelica, Fangji, chuanxiong, hippocampus, nepeta, papaya, licorice, myrrh, camphor , methyl salic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/60
Inventor 林冶
Owner 黄石卫生材料药业有限公司
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