17results about How to "Rich research" patented technology

The invention relates to a brain image generation method and system based on multilevel structure constraint, and a medium. The generation method comprises the following steps: preprocessing brain MRI image data; generating a high-fidelity cross-modal brain image through decoupling content features and attribute features by using a generative adversarial network framework of decoupling and comparative learning; pixel-level granularity discrimination: discriminating pixel-level features by using a brain memory library to enhance discrimination ability and optimize a memory space; and structure-level granularity discrimination: constraining different brain structures by dynamically adjusting a weight strategy so as to improve the learning effect of samples difficult to recognize. Compared with the prior art, the hierarchical granularity discrimination method provided by the invention aims to solve the problem of neglecting spatial coherence of structural hierarchical representation and anatomical consistency of content in a model training process by utilizing multi-level semantic information in a medical image.

The invention relates to the field of interdisciplinary, in particular to a subject interdisciplinary measurement method based on keywords. The measurement method mainly carries out analysis from three dimensions: semantic analysis: processing a corpus in two modes to obtain a matrix and a vector sequence, and carrying out cosine calculation on the matrix and the vector sequence to obtain an index reflecting the similarity of subject knowledge; the hierarchical structure analysis is to analyze and determine subject keyword contribution degree and quantify knowledge distance based on a hierarchical structure, and calculate the subject keyword contribution degree and the knowledge distance to obtain an interdisciplinary measurement index; the network structure analysis comprises the following steps: researching a keyword network to obtain an influence index of a node in the network, applying the index to multiple subjects, and calculating to obtain an interdisciplinary intensity measurement index based on a network structure; and finally, carrying out multi-index synthesis on the three dimensions through an entropy weighting method so as to carry out evaluation on the interdisciplinary interaction strength. According to the method, the problems of insufficient fine granularity and insufficiency in current subject cross detection are solved.

The invention relates to a gene diagnosis technology, in particular to a kit for detecting phenylketonuria pathogenic genes and application of the kit, the kit comprises a reagent for detecting PAH pathogenic mutants, and the PAH pathogenic mutants are characterized in that in a coding region of a nucleotide sequence shown as the accession number NM000277.3, the 721st basic group is mutated into C from T, and the 741st basic group is mutated into G from T; the reagent is a primer. According to the invention, a new mutation site of the phenylketonuria virulence gene is found, and the research of the phenylketonuria virulence gene is enriched; the kit provided by the invention contains a reagent for detecting the new mutation site, is found for the first time in the prior art, is simple in structure, can more conveniently and quickly detect the pathogenic gene of phenylketonuria, and increases a new clinical detection product.

The application discloses a test method and test bench for evaluating brake noise of a friction brake, comprising: carrying out brake noise test according to a set working condition, collecting brake torque, working speed and noise signal; obtaining speed-torque mean value characteristics and fluctuation characteristics based on the brake torque and the working speed, obtaining sound pressure level and noise growth rate based on the noise signal; and performing vibration noise performance evaluation based on the speed-torque mean value characteristics, the fluctuation characteristics, the sound pressure level and the noise growth rate. The application realizes collection of brake vibration noise related signals under different working conditions, and can also increase evaluation dimensions of vibration noise behavior based on vibration noise signals in test results, so as to fully reflect current noise performance and vibration stability characteristics of the brake.

The invention provides a device and a method for monitoring an expansion process of a hydrate-containing sediment crack, the device comprises an injection system, a reaction kettle, a temperature control system, an automatic pressure return system, a vacuum-pumping system, a data processing system and a non-contact strain observation system, a visual upper plug and a visual pressure head of the reaction kettle are combined to form a visual window; the non-contact strain observation system comprises a high-speed camera, and the high-speed camera is located above the visual window and electrically connected with the data processing system. According to the method, the crack initiation and expansion rule of the hydrate-containing sediment sample in the reservoir transformation process can be intuitively obtained; the method comprises the following steps: capturing a crack initiation point position, a crack extension direction and an evolution rule of a micro-crack on an injection surface in a fracturing crack-making process within an extremely short time, and acquiring strain change on the fracturing fluid injection surface after fracturing fluid injection and before a sample cracks; in combination with an acoustic emission monitoring means, the crack distribution characteristics in the hydrate-containing sediment sample can be further analyzed.

The invention discloses a chicken primordial germ cell transfection method which comprises the following transfection steps: S1, separating chicken primordial germ cells, and culturing the chicken primordial germ cells in a culture medium in groups, S2, mixing pPB-GFP transposon plasmids and transposase plasmids mPB according to a ratio of 3.5: 1, S3, diluting the plasmids by using opti-MEN, diluting a transfection reagent by using opti-MEN, and carrying out transfection by using opti-MEN. According to the method, the transfection operation of the chicken primordial germ cells is carried out by setting culture media with different serum addition amounts and different serum addition times, so that the influence of different serum addition amounts and different serum addition times on the transfection efficiency of the chicken primordial germ cells can be known; and then the increase and decrease of the fluorescence brightness in the transfected chicken primordial germ cells are recorded, and the growth condition of the transfected chicken primordial germ cells is known, so that the most suitable culture medium can be selected, the serum adding condition can be optimized, and more efficient transfection of the chicken primordial germ cells can be realized.

A simple earth surface crack monitoring device comprises a monitoring ruler used for measuring the width of a crack, and a first slide way is arranged in the middle of the monitoring ruler; the measuring plate is slidably connected to the first slideway; the extending assembly is arranged at the bottom of the measuring plate; the pulling mechanism drives the measuring plate to move; the positioning mechanism is slidably connected to the side wall of the monitoring ruler, and the bottom of the positioning mechanism is anchored to the ground surface. The change distance of the crack can be determined by monitoring the change of the distance between the two sliding blocks, the data relationship between the earth surface movement and the crack movement can be obtained by comparing the data with the data measured by the measuring plate, and the crack research is more comprehensive by increasing the test parameters; meanwhile, when the crack is too thin and the clamping piece cannot extend into the crack, early earth surface movement data can be measured in advance through movement of the sliding block.

The present invention relates to the field of medical information processing technology, and in particular to a method and system for automatically extracting knowledge in the field of critical illness. The method comprises the following steps: constructing a critical illness ontology in the field of critical illness to obtain a critical illness ontology data set; performing high-attention ontology mining on the critical illness ontology data set to obtain a high-attention ontology data set; performing topic consistency link analysis based on the high-attention ontology data set to obtain a potential critical illness knowledge source set; performing temporal knowledge fluctuation analysis on the potential critical illness knowledge source set to obtain a dynamic knowledge update time span data set; performing frontier knowledge source hierarchical screening on the potential critical illness knowledge source set based on the dynamic knowledge update time span data set to obtain a critical illness frontier dynamic knowledge source set. The present invention reduces the burden of manual search and literature reading, and improves the efficiency and comprehensiveness of knowledge acquisition.

The present invention belongs to the field of bioinformatics, and specifically relates to a method and system for predicting the risk of tumor cell metastasis. The method of the present invention predicts the S of the tumor cell to be detected based on the gene expression information of the tumor cell to be detected. EMT , S Tra and S comm ; Among them, S EMT Used to indicate the epithelial-mesenchymal transition ability of tumor cells, S Travel Indicates the survival ability of tumor cells in blood circulation, S comm Indicates the ability of tumor cells to invade tissues or organs; then calculate the risk factor S of the tumor cells to be tested for metastasis Merta The method of the present invention can assist doctors in timely diagnosis and targeted treatment of cancer patients in the early stage of cancer metastasis, and can guide the research and development of new anti-cancer drugs, which is conducive to improving the prevention and treatment rate and cure rate of cancer.

This invention discloses a light angle sensor and a light angle sensing method. The light angle sensing method includes the following steps: (1) Based on a one-dimensional double-layer YIG photonic crystal cell, the spacing between the scatterers in the upper and lower layers is changed to generate two structural units: Type I (near top, far bottom) and Type II (far top, near bottom); (2) The spacing between the scatterers is adjusted so that the energy bands of the two structures are the same, and then four cells from each structure are spliced ​​together to form a parallel plate waveguide, forming a topological interface in the middle; (3) The waveguide energy state number is calculated, the band gap with two boundary states is found, the transmission spectrum is calculated, and two Fano curves are formed; (4) The light incident angle is changed, and the relationship between the total extinction ratio and the incident angle of the two Fano curves is calculated. The curves show the high sensitivity sensing characteristics of the light incident angle. This method not only enriches the research on topological boundary states, but also provides a simpler method for realizing high-sensitivity light angle sensing.

The invention discloses a test device and method for simulating loess moisture migration under a dry-wet cycle. The test device comprises a test box, an environment simulation module, a moisture migration characteristic parameter measurement module and a lost soil particle collection module, the test box is of a three-layer combined structure, and the middle layer is an independent sample groove; the environment simulation module comprises a rainfall simulation unit and a temperature simulation unit; the rainfall simulation unit comprises a main water inlet pipe, a water tank and a rainfall nozzle; the temperature simulation unit comprises a heating belt, an air blower, an air inlet pipe, a temperature sensor and an air outlet; the temperature sensor is arranged on the side wall of the upper layer of the test box; the air outlet is formed in the top of the test box; the moisture migration characteristic parameter measurement module comprises a soil moisture temperature sensor, a soil moisture sensor, a soil pressure gauge and a pore water pressure gauge which are buried in multiple layers in the soil sample in the sample groove; the lost soil particle collection module comprises a step-by-step filtering device and a laser particle size distribution instrument. The problem of how to reveal the deep mechanism of the occurrence of loess disasters is solved.

The invention belongs to the field of biological medicines, and discloses a shearing inhibitor for pre-miR-21 post-transcriptional processing as well as a preparation method and application of the shearing inhibitor. The shearing inhibitor for pre-miR-21 post-transcriptional processing is a benzimidazole derivative with a structure as shown in the following formula I. The invention further discloses a preparation method of the shearing inhibitor for pre-miR-21 post-transcriptional processing. The shearing inhibitor for pre-miR-21 post-transcriptional processing can be combined with pre-miR-21 in vivo and in vitro and inhibit shearing of pre-miR-21 post-transcriptional processing, so that expression of miR-21 is silenced, modified and / or improved, and finally growth of human breast cancer cells MDA-MB-231 is inhibited in vivo; the shearing inhibitor processed after the pre-miR-21 transcription is good in stability, convenient to store, low in cost and good in anti-tumor effect. # imgabs0 #

The application belongs to the technical field of plant genetic engineering, and particularly relates to a cyclic nucleotide-gated ion channel OsCNGC10 gene for regulating rice lodging resistance and drought resistance and application. A cyclic nucleotide-gated ion channel OsCNGC10 gene induced by drought is screened, and the nucleotide sequence is shown as SEQ ID NO:1; the protein sequence coded by the gene is shown as SEQ ID NO:2. An OsCNGC10 overexpression strain and a CRISPR / Cas9 mutant strain are obtained by using an agrobacterium-mediated transformation. Stem characteristics and lodging resistance analysis of the transgenic plants show that the mutant oscngc10-2 has enhanced stem strength and lodging resistance; overexpression of OsCNGC10 reduces the stem wall thickness and lignin content, and knockout of OsCNGC10 increases the stem lignin content, which preliminarily indicates that OsCNGC10 negatively regulates the rice lodging resistance. The results of the drought stress experiment in the seedling stage show that the MDA accumulation speed in the OsCNGC10 gene-deficient plants is accelerated, and the plants cannot form sufficient free proline; the free Pro content in the overexpression OsCNGC10 plants is greatly increased, and the MDA accumulation speed is relatively slow, which indicates that OsCNGC10 positively regulates the rice seedling drought resistance.

The present invention discloses a molecular marker for early flowering traits of Brassica napus, a method for obtaining the marker and an application thereof. The mutant ebm induced by EMS in early flowering materials of rapeseed is identified through field flowering period survey, and an F2 segregation population of the mutant and double 9 in late flowering source materials is constructed. The early flowering gene is initially located by using the mixed pool resequencing (BSA‑seq) technology, and SNP molecular markers that can be used for early flowering trait screening and gene positioning are screened in this interval. The molecular markers include SNP molecular markers 1‑11 that are homozygous for both parents. The present invention provides new molecular markers for the excavation of flowering mechanism and molecular breeding of rapeseed, and has important application prospects for enhancing seed purity and the selection of flowering period materials in the hybrid breeding process of rapeseed.

The present invention discloses an FtsZ protein inhibitor with antibacterial effect and its application, belonging to the field of biotechnology. The FtsZ protein targeted inhibitor of the present invention includes compound BP210517 and compound BP210914; the structural formulas of the compound BP210517 and the compound BP210914 are shown in formula (1) and (2), respectively. The present invention also provides the application of the compound in the preparation of a drug for inhibiting FtsZ protein, and the application of the compound in enhancing the effective dose of antibiotics and enhancing the antibacterial activity of antibiotics. The disclosure of the BP210517 and BP210914 compounds of the present invention enriches the research field of targeted FtsZ antibacterial agents, can be used as lead compounds of potential antibacterial drugs, realize clinical applications, and have the value of further transformation.

This invention belongs to the field of plant genetic engineering technology, specifically involving the OsRAV9 gene, a transcription factor that regulates drought resistance in rice, and its application. A drought-induced expression of the OsRAV9 gene was screened, its nucleotide sequence being shown in SEQ ID NO:1; the protein sequence encoded by this gene is shown in SEQ ID NO:2. Using Agrobacterium-mediated transformation, OsRAV9 overexpressing transgenic lines and CRISPR / Cas9 mutant lines were obtained. Seedling drought stress experiments showed that under drought stress, the accumulation rate of MDA in OsRAV9 gene-deficient plants was accelerated, and sufficient free proline could not be formed. In contrast, OsRAV9 overexpressing plants showed a significant increase in free proline content and a relatively slower accumulation rate of malondialdehyde (MDA) under drought stress, preliminarily indicating that OsRAV9 positively regulates drought resistance in rice seedlings.

The present invention discloses a method for dividing and identifying industrial development stages based on characteristic statistical measurement, including: constructing an industrial stage development characteristic indicator system; performing statistical measurement on the industrial stage development characteristic indicators: collecting, processing and statistically measuring indicator data, and identifying industrial development stages based on this division, wherein the statistical measurement first divides the industrial development stages through clustering measurement, and then identifies the industrial development stages through structural measurement, and obtains the final division and identification results of the industrial development stages based on the statistical measurement; the present invention constructs an industrial stage development characteristic indicator system, performs statistical measurement on the indicator system, and identifies the rail transit industry development stages based on this division, and obtains corresponding conclusions.