571 results about "Experimental model" patented technology

Variant human MLH1 and MSH2 genes are provided. Methods of using these variant genes to diagnose hereditary non-polyposis colorectal cancer (HNPCC) and/or determine a patient's susceptibility to developing HNPCC are also provided. Methods and compositions for identifying new variant MLH1 of MSH2 genes are also provided. In addition, experimental models for hereditary non-polyposis colorectal cancer comprising these variant genes are provided.

An optical metrology system includes model approximation logic for generating an optical model based on experimental data. By eliminating theoretical model generation, in which the fundamental equations of a test sample must be solved, the model approximation logic significantly reduces the computational requirements of the metrology system when measuring films formed on patterned base layers. The experimental model can be created by selecting an expected mathematical form for the final model, gathering experimental data, and compiling a lookup model. The lookup model can include the actual measurement data sorted by output (attribute) value, or can include “grating factors” that represent compensation factors that, when applied to standard monolithic model equations, compensate for the optical effects of grating layers.

Method of cyclic anchoring itinerant (CAI) propulsion uses a phenomenon of anchored float horizontal motion as wave rises. The method repeats a cycle: Detaching the anchor from an immobile medium as a wave begins descent; Accelerating it in new position as the wave descends; Reattaching it to said medium as a wave rises. The medium can be railway or water. Respectively a trolley or a glider works as the anchor. To drag as anchor the glider orients perpendicular to the link. Improvements: adding an auxiliary propeller accelerating the glider, connecting bow and aft gliders with a single boom, making it telescopic, adding a small submarine hull for permanent observation water space, scientific researches, entertainment. The method allows to reequip watercrafts to all-weather, ocean going watercrafts with an unlimited range of operation. These solutions can be widely used in marine rescue remedies. Experimental models of CAI propulsion system show remarkable results.

The invention provides a simulation test system and a simulation test device of train network control software. The simulation test device comprises a man-machine interaction unit, a controlled plant creating unit, a vehicle-mounted electrical connection creating unit, a simulation interface creating unit and a simulation control unit, and the simulation test system of the train network control software comprises at least one group of pull-in control unit test systems; each pull-in control unit test system comprises the simulation test device and at least one set of network control system core equipment which comprises a central control unit, the central control unit is switched into a vehicle bus and a train bus, and the simulation test device is switched into the vehicle bus; and the central control unit is electrically connected with a hard wire of the simulation test device, and the simulation test devices in two or more than two pull-in control unit test systems are connected through an Ethernet. The simulation test system and the simulation test device provided by the invention can directly service development and test of the train network control system software, and can quickly construct an experimental model, shorten development cycle, and reduce economic cost.

The invention relates to a yageine derivative and salts of compounds. The invention synthesizes the novel yageine derivative compounds which can strengthen the antineoplastic activity and reduce the toxicity of a nervous system through the structural modification to locus 1, 2, 3, 7 and 9 of beta-carboline parent nuclei of yageine. The compounds can be used for preparing drugs used to treat various tumor diseases through the screening study of in vivo and in vitro anti-tumor experimental models.

The invention relates to a condensate gas reservoir blockage relieving physical simulation experimental device and a condensate gas reservoir blockage relieving physical simulation experimental method, and belongs to the technical field of oil field development experiment. The experimental device consists of a condensate gas reservoir model, an injection system, a production system and a data analysis system. The experimental device is used for performing preparation for simulating a rock core of a gas layer main model and simulating a rock core of a near wellbore zone model; the prepared rock cores are subjected to vacuum pumping and saturated gas condensation; a condensate gas reservoir blockage relieving experimental model is built; condensate gas reservoir seepage flow passage blockage simulation experiments and blockage relieving simulation experiments are performed; a condensate gas reservoir blockage relieving scheme is made according to obtained experimental data; the stratum temperature, the pressure and the depletion development process of the condensate gas reservoir can be simulated in a laboratory; the dynamic simulation of the depressurizing production and the gas injection swallowing-spitting blockage relieving of the condensate gas reservoir is realized; the blockage relieving gas injection quantity and the injection period of the simulated condensate gas reservoir are determined; the operation is simple and convenient; the implementation is easy; and the investment of a large amount of labor, materials and money on site is not needed.

The invention discloses a multi-scale porous structure light weight modeling method oriented to 3D printing, and belongs to the field of computer aided design and industrial design manufacture. Undera condition that characteristic constraints and a stress condition are given, through a compact support radial basis function interpolation, a smooth multi-scale porous model is constructed; the multi-scale porous model is applied to light weight modeling, and a feasible solving solution is given; through the 3D printing, an entity experiment model is obtained and is subjected to engineering stress verification; according to engineering verification result analysis, a parameter is corrected to enable the hole change of an optimization model to more approach to practical stress requirements; and through the above loop iteration process, a light weight model which meets stress requirements is obtained. By use of the method, a light weight purpose of the entity model can be truly realized, sothat the light weight design optimization period of the model is greatly shortened, the porous structure obtained by design has the advantages of smoothness, full connectivity, controllability and quasi-self-supporting, and the effectiveness and the manufacturability of light weight can be accurately guaranteed.

The invention relates to an unsteady cavitation flow-excited vibration multi-field synchronous measurement system for a water tunnel experiment and belongs to the ship and underwater vehicle engineering and water conservancy and hydropower engineering technology field. The system is composed of an experimental section, an experimental model, a high-speed camera, a synchronous trigger switch, a laser Doppler vibration meter, a data acquisition instrument, a data processing system/computer and display assembly and a light source. According to the system of the invention, rising edge voltage signals are generated through the synchronous trigger switch, trigger signals are controlled by using the voltage signals, and therefore, the signal acquisition starting instructions of the high-speed camera and the laser Doppler vibration meter are controlled quantitatively and precisely, and the quantitative and accurate synchronous measurement of an unsteady cavitation flow field and a structure field can be realized; and data obtained through multi-physical field synchronous acquisition are subjected to preprocessing, time-domain analysis, frequency-domain analysis and time-frequency analysis,and therefore, reliable analysis experimental data can be provided for the study of fluid-structure interaction characteristics.

The invention relates to a method for apperceiving and reconstructing a non-vision structural form of a near space vehicle model. The method comprises the following steps: on the basis of a distributed embedded dispersed bragg fiber grating sensing array, sensing and detecting strain information of each measuring point of a test model, and utilizing a certain curvature conversion algorithm to convert the strain information into curvature information; on the basis of a curvature interpolation, calculating the related coordinate value of each point of wings and frame of the test model according to a three-dimensional space curve fitting algorithm of orthogonal curvature information; utilizing a coordinate fuse algorithm to obtain an absolute coordinate value of each point of the model; and lastly, reconstructing the deformation state and the low-frequency vibrating form of the test model according to a computer graphic processing technology and realizing the three-dimensional visual real-time display for the change in the model form. An experimental system for the method comprises a simulated near space vehicle model, a fiber grating network analyzer with a network service function, a high-performance computer and a display, wherein the fiber grating sensing array is embedded into the surface of the simulated near space vehicle model.

The invention discloses a similar material proportion determining method used in a physical simulation experiment. The similar material proportion determining method used in the physical simulation experiment includes the steps that (1) physically similar materials of a typical property rock stratum are selected, a series of physically similar materials are made according to the different proportions of aggregates, cementing materials and additives, and a database is built; (2) a physical simulation experiment model is selected, and the types of the physically similar materials and the similarity proportion are determined; (3) each simulation rack stratum mechanical parameter of the physical simulation experiment model is determined; (4) the types, proportion and detailed consumption amounts of the physical simulation experiment model are determined; (5) a physically similar material proportion scheme is generated, and experiment model pavement is carried out; (6) an indoor experiment is carried out according to the physical simulation experiment scheme, evaluation values are analyzed, the physical proportion scheme is corrected, and each mine stratum physically similar material proportion database is built. The similar material proportion determining method used in the physical simulation experiment is simple, standard, reasonable and feasible in step, and capable of effectively changing the situations that standardability is poor in the experiment, too much qualitative analysis is needed, and accuracy and repeatability are poor.

The invention discloses a dynamic water quicksand stratum reinforcement grouting diffusion rule visual simulation experimental device and method. The device comprises an experimental box, a hydraulic pressure control loading system, a vertical hydraulic loading mechanism and a lateral hydraulic loading mechanism, a double liquid chemical grouting system and a test system connected to a computer. The method adopts an engineering geomechanical experimental model with a coal measure stratum physical analog simulation material, a waterproof stratum and an aquifer of certain height filled in the experimental box, aquifer grouting and water-plugging reinforcement visual physical simulation test is carried out hermetically under the conditions of circulating dynamic water pressure and vertical and horizontal lateral stress to acquire or quantitatively describe and analyze the diffusion rule of double liquid chemical grout in the water-bearing sandstone layer or quicksand layer, thus realizing visual simulation experiment of the double liquid component chemical grouting and water-plugging process under a similar water burst or water inrush condition. The method can effectively recognize and reveal the slurry diffusion and consolidation characteristics under a mining dynamic water condition, determine rational grouting and water-plugging parameters, and evaluate the grouting effect.

The invention discloses a synchronous data acquisition system used in a wind tunnel based on stable dynamic pressure control. The data acquisition system comprises a pressure sensor, a pressure acquisition system, an aerodynamic force balance unit, an aerodynamic force acquisition system, a standard pressure source, an industrial personal computer (IPC), a stable dynamic pressure sensor, an accuracy control device, an aerodynamic parameter system and a motor fan, wherein, the pressure sensor measures the pressure in the low-speed wind tunnel and transmits the measured pressure value to the pressure acquisition system; the aerodynamic force balance unit measures aerodynamic force on an experimental model and outputs the measured aerodynamic force value to the aerodynamic force acquisition system; the standard pressure source respectively calibrates the pressure sensor, the pressure acquisition system and the stable dynamic pressure sensor so as to obtain calibration factors, the calibration factors are transmitted to the IPC so as to obtain a controlled process mathematical model for stable dynamic pressure control, and then the controlled process mathematical model is transmitted to the accuracy control device; the accuracy control device drives the pressure acquisition system, the aerodynamic parameter system and the aerodynamic force acquisition system to perform synchronousdata acquisition; the stable dynamic pressure sensor acquires variation of a flow field in the low-speed wind tunnel, and then the variation value is transmitted to the IPC; and the IPC controls the motor fan to rotate. The data acquisition system disclosed by the invention has the advantages that manpower is saved, high-accuracy data can be obtained, the operating method is simple and practical,the cost is low and the application prospect is wide.

The present invention relates a method for isolating four cell types from a single umbilical cord as pure cultures. These cell lines (epithelial cells, fibroblasts, smooth muscle cells and endothelial cells) can be characterised and utilised in experimental models and for therapeutic purposes. Particularly, the umbilical cells isolated herein are used to form a tissue replacement or engineered living composition. Also, the isolated umbilical cells of the invention may have the potential of progenitor cells.

The invention provides an experiment device and an experiment method for simulating thermal jet flow interference. The experiment device comprises a hypersonic wind tunnel, an experiment model, a bracket, a Laval internal nozzle, a model nozzle, a heater, an air storage tank, an air cylinder and a CF4/SF6 gas cylinder, wherein an air source is compressed and expanded through the hypersonic wind tunnel and then becomes hypersonic airflow to provide an experiment with external flow; a CF4/SF6 gas is mixed with air and then provides the experiment with internal jet flow; the specific heat ratio required by the experiment is obtained through controlling the molar mixing ratio and the temperature of the CF4/SF6 gas and air, so that the requirements on specific heat ratio simulation of the jet flow in the experiment are met; and simulation of a jet flow interference experiment of the specific heat ratio of the jet flow is achieved in a common wind tunnel. The experiment method has the advantages of being relatively long experiment time, free of pollution, low in thermal load and low in cost, and therefore, the experiment method can be used as an effective means for evaluating the performance of the spray pipe of a hypersonic aerocraft.

A digested phagocyte prepared by contacting in vitro a phagocyte with a foreign microorganism and isolating the phagocyte so contacted; a process for producing the same; and a process and a kit in which these are utilized are disclosed. An experimental model, which enables in vitro evaluation of a phagocytotic function of phagocytes, is provided.

Highly efficient gene transfer into primate-derived embryonic stem (ES) cells has successfully been achieved by using a simian immunodeficiency virus vector (SIV) pseudotyped with VSV-G protein, which is a surface glycoprotein of vesicular stomatitis virus (VSV) The present invention provides simian immunodeficiency virus vectors for gene transfer to primate ES cells. The method for gene transfer to primate ES cells using the vectors of the present invention is useful in, for example, research into embryology and disease, clinical applications, and experimental models for primates. The method is also useful in assaying and screening for genes and reagents able to enhance the specific differentiation of tissues or cells, and which are useful in preparing desired cells or tissues differentiated from ES cells.

The invention discloses a stress and displacement continuous measuring method for coal mine similar simulation experiments.The method comprises the steps that on the basis of a similar simulation experiment model, under the effect of similar simulation experiment loads, stress and displacement changes of a model rock stratum before breaking are automatically and continuously measured and recorded, and whole-process data measurement of stress and displacement of a similar simulation model overlying rock layer before breaking is achieved.According to the adopted similar simulation model, the structure is simple, use is convenient, stress and displacement measurement is accurate and reliable, stress and displacement change whole-process data of the model rock layer before breaking is automatically and continuously measured and recorded in the similar simulation experiment process, and an early-stage theoretical foundation is provided for coal mine design mining construction.

The invention discloses an inclination angle measurement error data correction system and method of an experimental model in a wind tunnel when the experimental model is placed in the wind tunnel to be subjected to a blowing experiment. The inclination angle measurement data correction system comprises a data preprocessing module, a model training module and an error data correction module, and the correction method comprises three steps: data preprocessing, model training and error data correcting. The invention provides a multi-stage and multi-model fusion error correction method based on a neural network model as a basic model, the model fitting degree is higher, the calculation result is more accurate, the nonlinear error, caused by vibration of the model under the wind action, in data measured actually by an inclination angle sensor can be corrected well, and the correction error of the correction system can be controlled within 0.004-0.01 degree near the 0 degree.

The invention discloses a low-speed wind tunnel virtual flying experimental model attitude measuring system. The system comprises an airborne power supply, an embedded attitude measuring computer, a heading attitude reference system, an inertial measuring unit and a system calibration computer; the airborne power supply, the embedded attitude measuring computer, the heading attitude reference system and the inertial measuring unit are arranged inside an aircraft model; measuring information of the heading attitude reference system and measuring information of the inertial measuring unit enter the embedded attitude measuring computer through a serial bus; a computing result of the embedded attitude measuring computer is sent to the system calibration computer through the Ethernet in a wireless mode; the system calibration computer is arranged outside a wind tunnel experimental section. According to the low-speed wind tunnel virtual flying experimental model attitude measuring system, the angular velocity and attitude angle of the model are directly measured through pure inertial measuring elements, an air flow angle is indirectly calculated and obtained by utilizing a transformational relation of the attitude angle peculiar to a virtual flying experiment and the air flow angle, and therefore attitude measuring information of the virtual flying experimental model is obtained.

The invention relates to an HUVEC (human umbilical vein endothelial cell) separation, culture and subculture method belonging to the technical field of biology. The method comprises the following steps: (1) separation of HUVEC: taking a fresh umbilical cord, cleaning with a sterile PBS (phosphate buffer solution) containing penicillin-streptomycin, adding collagenase to perform water bath digestion at 37 DEG C, centrifuging the digestion solution, then adding an M199 culture medium, transferring into a gelatin-coated culture bottle, and culturing in a CO2 incubator at 37 DEG C; (2) culture of HUVEC: after the HUVEC is cultured at 37 DEG C for 24 hours, pouring out the culture medium, cleaning with the PBS, adding a fresh M199 culture medium, and afterwards, changing the culture medium once every two days, wherein the HUVEC can be subcultured generally after being cultured for 5-7 days; and (3) subculture of HUVEC: pouring out the culture medium, cleaning with the PBS, adding the digestion solution to digest the cell, adding a DMEM (dulbecco's modified eagle medium) containing serum to terminate the reaction once the cell is rounded, centrifuging the digested cell, and adding a fresh culture medium, wherein the HUVEC subcultured for 2-3 generations is used for experiments. The separated HUVEC is economical and practical, is simple and easy to use, and is beneficial to obtaining required in-vitro experimental model cells.

The invention belongs to the field of hydraulic experimental model facilities and particularly relates to an automatic control type circulating water tank for simulating the instantaneous dam break. The circulating water tank comprises a water tank, a gate, a water level control system and a gate lifting control system. The water level control system comprises a water injection pipe, a water inlet pipe and a water guide pipe which are connected through a three-way pipe fitting, wherein the water guide pipe is connected with a circulating water pump. The water level control system further comprises a gate upstream water level gauge and a gate downstream water level gauge, wherein the gate upstream water level gauge and the gate downstream water level gauge are respectively arranged in a gate upstream water tank and a gate downstream water tank. The gate lifting control system comprises a vertical rack arranged on the front surface of a gate door plank and matched with the driving gear of a motor, and a lifting guide rail fixedly erected on the water tank along a guide rail. During the lifting process of the gate door plank, a pair of vertical guide bars on the back surface of the gate door plank are always fittingly kept in the vertical guide grooves of the lifting guide rail. An automatic air drying device is arranged in the gate downstream water tank, so that the conduction of dry bed experiments is facilitated. The circulating water tank is stable, automatic and efficient.