1186 results about "Rectal carcinoma" patented technology

Variant human MLH1 and MSH2 genes are provided. Methods of using these variant genes to diagnose hereditary non-polyposis colorectal cancer (HNPCC) and/or determine a patient's susceptibility to developing HNPCC are also provided. Methods and compositions for identifying new variant MLH1 of MSH2 genes are also provided. In addition, experimental models for hereditary non-polyposis colorectal cancer comprising these variant genes are provided.

The present invention provides methods and materials related to the detection of colorectal neoplasm-specific markers (e.g., markers associated with colorectal cancer, markers associated with adenoma) in or associated with a subject's stool sample. In particular, the present invention provides methods and materials for identifying mammals (e.g., humans) having a colorectal neoplasm by detecting the presence and level of indicators of colorectal neoplasia such as, for example, long DNA (e.g., quantified by Alu PCR) and the presence and level of tumor-associated gene alterations (e.g., mutations in KRAS, APC, melanoma antigen gene, p53, BRAF, BAT26, PIK3CA) or epigenetic alterations (e.g., DNA methylation) (e.g., CpG methylation) (e.g., CpG methylation in coding or regulatory regions of bmp-3, bmp-4, SFRP2, vimentin, septin9, ALX4, EYA4, TFPI2, NDRG4, FOXE1) in DNA from a stool sample obtained from the mammal.

Inhibitors of Ras protein, methods to modulate the activity of Ras protein, and methods of treatment of disorders mediated by Ras protein are provided. A method for regulating activity of a K-Ras, H-Ras or N-Ras mutant protein with a compound is described. Disorders that can be treated include cancer, such as hematological cancer, pancreatic cancer, MYH associated polyposis, colorectal cancer, or lung cancer.

The invention discloses a screening method of colorectal cancer treatment prognosis biomarkers. The screening method includes the following steps of: (1) making a primary search strategy for screening the biomarkers; (2) making a literature adopting and exclusion criterion; (3) analyzing data and primarily screening the biomarkers; (4) making evident grades; (5) making a high quality evident evaluation criterion; (6) performing grading retrieval and quality evaluation on biomarker clinic evident; (7) performing data analyzing and statistics on the biomarker clinic evident; (8) screening the prognosis biomarkers. The invention further provides the colorectal cancer treatment prognosis biomarkers obtained through screening according to the screening method, and the prognosis biomarkers include KRAS, TYMS, TYMS, EGFR, UGT1A1*28, DPYD, PIK3CA, ERCC1, PTEN, VEGFR, BRAF, GST P1, XRCC1, MTHFR, MSI and the like.

The described invention relates to the identification of biomarkers for gastrointestinal diseases and provides methods utilizing the biomarkers for in drug discovery, monitoring of treatment efficacy, and diagnostics. The invention further provides methods for identifying a therapeutic target to treat ulcerative colitis, colorectal cancer, and Crohn's disease.

The invention is directed to methods and kits that allow for classification of colorectal cancer cells according to genomic profiles, and methods of diagnosing, predicting clinical outcomes, and stratifying patient populations for clinical testing and treatment using the same.

Conformationally constrained compounds that mimic the secondary structure of reverse-turn regions of biologically active peptides and proteins are disclosed. Such reverse-turn mimetic structures have utility over a wide range of fields, including use as diagnostic and therapeutic agents. Libraries containing the reverse-turn mimetic structures of this invention are also disclosed as well as methods for screening the same to identify biologically active members. The invention also relates to the use of such compounds for inhibiting or treating disorders modulated by Wnt-signaling pathway, such as cancer, especially colorectal cancer, restenosis associated with angioplasty, polycystic kidney disease, aberrant angiogenesis disease, rheumatoid arthritis disease, tuberous sclerosis complex, Alzheimer's disease, excess hair growth or loss, or ulcerative colitis.

The invention relates to a colorectal cancer diagnostic biomarker and application thereof. A nucleotide sequence is shown as SEQ ID NO. 1. The colorectal cancer diagnostic biomarker provided by the invention has the beneficial effects that hsa_circ_0006110 gene expression is discovered to be closely related to a colorectal cancer for the first time; through detecting the expression of hsa_circ_0006110 in a subject colorectal tissue, whether a subject suffers from the colorectal cancer or the risk of the colorectal cancer exists or not can be more accurately and quickly judged, so that a prevention or treatment scheme is provided for a clinician. A target gene as a drug for treating the colorectal cancer provides a new treatment targets and treatment approach for colorectal cancer treatment; compared with a traditional detection means, the diagnostic biomarker is more timely and more specific to diagnose, so that the five-year survival rate of a colorectal cancer patient is improved, and the death rate is reduced, therefore, the application prospect is wide.

The present invention relates to the use of myeloid cell biomarkers for the differential diagnosis, prognosis, and monitoring of renal cell carcinoma (RCC) or colorectal cancer (CRC). The present invention furthermore relates to monitoring the effect of a treatment against renal cell carcinoma (RCC) or colorectal cancer (CRC), and establishing a prognosis of the outcome of the treatment of renal cell carcinoma (RCC) or colorectal cancer (CRC). The present invention furthermore relates to panels of cellular biomarkers for use in the above methods, in particular multicolor panels for measuring said biomarkers.

The invention relates to a method for calculating a flora balanced relation index in an individual excrement sample, and an application of the method in screening, diagnosis or auxiliary diagnosis incolorectal cancer (CRC). By extracting bacteria in excrement during DNA sequencing, the types and quantity characteristics of the bacteria can be obtained, and a CRC diagnosis by taking quantity ratiocharacteristic of a plurality of bacteria as a base is carried out. Compared with the methods used in clinical diagnosis or noninvasive screening of CRC with an applied patent, the method is completely noninvasive, and can realize accurate diagnosis of CRC. The analysis result displays that a ratio of fusobacterium nucleatum Fn to bifidobacteria Bb quantity (Fn/Bb) has high susceptibility and specificity on CRC screening, which can respectively reach 84.6% and 92.3% (AUC=0.911). the ratio of fusobacterium nucleatum Fn to clostridium leptum Fp (Fn/Fp) quantity is combined to increase the diagnosis value on CRC, and the Area Under Curve (AUC) of a subject work characteristic curve can reach 0.943. In addition, combination of Fn/Bb and Fn/Fp quantity ratio for screening I-stage CRC has 60% of specificity and 90% of sensitivity.

The invention provides a new long noncoding RNA (LncRNA) Lnc21q22.11 sequence, and a primer pair and kit for detecting expression level of the long noncoding RNA in cells and tissues. The full length of the LncRNA Lnc21q22.11 is identified to be 1202bp for the first time, the specific primers are utilized for the first time to detect the expression level of the LncRNA Lnc21q22.11 in stomach cancer cells, 35 pairs of stomach cancer line and para-carcinoma tissues, colorectal cancer cell lines and 10 pairs of colorectal cancer and para-carcinoma tissues, and thus, the kit is creative. The detection of the expression of the LncRNA Lnc21q22.11 in the stomach cancer tissues by using the kit and specific primers can be used as an effective means for stomach cancer and colorectal cancer diagnosis, curative effect observation, prognosis judgment, focus residue and relapse detection and the like. The kit is simple to operate, has the advantages of high stability and high sensitivity, and has far-reaching clinical meanings and popularization performance.

The invention discloses a method and a system for discovering and integrating rectal cancer related genes by utilizing public data resources and analyzing functions of the rectal cancer related genes, and an application. Based on the public data resources, open big data resources and diversified bioinformatics analysis means are reasonably used for performing analysis processing on mRNA expression data, and important genes related to complex diseases and functions of the important genes are identified. The method comprises the steps of sample data downloading and management; gene expression data analysis; difference expression gene screening; and gene function analysis and protein interaction analysis. According to the method and the system, the problems of weakness in integrating existing network resources, unfamiliarity with mRNA related most common databases and frontal analysis methods, incapability of independently finishing mRNA expression spectrum related bioinformatics analysis and the like can be solved; a plurality of risk pathways and genes related to the complex diseases such as the rectal cancer and the like can be discovered; and the method and the system are of important significance for biological targeted treatment of the complex diseases, biological drug research and development, pathogenesis explanation and risk prediction.

The invention relates to the measurement for the lipid molecule content in a human blood sample as well as the preparation and the application of a colon cancer or rectum cancer diagnosis reagent kit, in particular to the extraction of lipid molecules in a human blood sample and the content measurement thereof as well as the definition of colon cancer or rectum cancer lipid molecule marks. In addition, the invention also discloses the details about the detection of a few colon cancer or rectum cancer lipid molecule marks in a blood sample and provides a reagent kit which is formed according to the lipid molecule marks and used for colon cancer or rectum cancer diagnosis. According to the content of the lipid molecule marks in the sample, the possibility for a patient suffering the colon cancer or rectum cancer can be diagnosed from the sample through special calculation and analysis, and the invention also provides a new means for the healing efficacy analysis in the colon cancer or rectum cancer treatment.

Conformationally constrained compounds that mimic the secondary structure of reverse-turn regions of biologically active peptides and proteins are disclosed. Such reverse-turn mimetic structures have utility over a wide range of fields, including use as diagnostic and therapeutic agents. Libraries containing the reverse-turn mimetic structures of this invention are also disclosed as well as methods for screening the same to identify biologically active members. The invention also relates to the use of such compounds for inhibiting or treating disorders modulated by Wnt-signaling pathway, such as cancer, especially colorectal cancer, restenosis associated with angioplasty, polycystic kidney disease, aberrant angiogenesis disease, rheumatoid arthritis disease, tuberous sclerosis complex, Alzheimer's disease, excess hair growth or loss, or ulcerative colitis.

The invention discloses a Caco-2 cell surface specific binding polypeptide. Four polypeptide fragments are screened by phage display of a random dodecapeptide library, and the amino acid sequences of the four polypeptide fragments are respectively as follows: SPSIDTRYSRLG, CVSVGMKPSPRP, SVSVGMKPSPRP and MVSMDSSPRDRL. According to the screening method disclosed by the invention, colorectal carcinoma Caco-2 cell binding polypeptide sequences are screened by a phage polypeptide display technology, the affinity of phage clones with colorectal carcinoma cells is judged by enzyme-linked immunosorbent assay (ELISA), ten phage clones are obtained, and four polypeptide sequences are obtained by sequencing. The consensus amino acid sequence is XXSXXXXXXXRXX; homology analysis indicates that the polypeptide motif is likely to be the amino acid determinant on the tumor cell surface receptor binding ligand protein; by further judgment of cell immunofluorescence, the targeting result of the phage positive clones implicates that the phage positive clones can be specifically bound with Caco-2 cells; and the colorectal carcinoma Caco-2 cell specific polypeptide acquired by screening provides a preliminary experiment basis for early diagnosis of colorectal carcinoma, targeted delivery of antitumor drugs and development of targeted small peptide drugs.

The invention provides an exosome miRNA marker for colorectal cancer diagnosis. The marker is selected from at least one of let-7b-3p, let-7b-5p, miR-150-3p, miR-145-3-, miR-139-3p, miR-139-5p, miR-15b-3p, miR-125a-5p, miR-140-5p, miR-342-3p, miR-132-5p and miR-92b-5p. The invention further provides a kit for colorectal cancer diagnosis. The invention further establishes a method for detecting themiRNA marker. A colorectal cancer diagnosis and recurrence monitoring method is developed on the basis of non-invasive detection technology, the exosome miRNA marker has high sensitivity and high specificity in early-stage colorectal cancer, AUC is up to 0.99 as multiple miRNA markers are combined, and the exosome miRNA marker has extremely excellent diagnosis performance.