151 results about "Lymph node" patented technology

The invention discloses a novel application of bifidobacterium longum subsp. Infantis CCFM1269 in relieving atopic dermatitis, and belongs to the technical field of microorganisms. The bifidobacterium longum subsp. Infantis CCFM1269 provided by the invention can relieve atopic dermatitis, and is specifically reflected in that (1) ear swelling and back skin thickening of mice are relieved, dermatological symptoms are relieved, and mast cell infiltration is reduced; (2) reducing the total IgE level of serum; (3) the Treg level of mesenteric lymph nodes and the ILA content of serum are improved; (4) IL-4 and TSLP levels in skin tissues are reduced, IL-10 is improved, and Th1 / Th2 immune balance is recovered; (5) improving AHR and CYP1A1 in the skin tissue; and (4) high yield of ILA.

The invention relates to the field of image analysis, in particular to a colon cancer lymph node metastasis risk prediction method and system based on image analysis. The method comprises the following steps: acquiring a pathological section scanning image, carrying out layer-by-layer downsampling and feature reconstruction, and generating pathological section topological features; carrying out adaptive clustering analysis and multi-parameter fusion evaluation on the basis of the pathological section topological features to obtain independent migration evaluation values of individual tumor cells; performing tumor metastasis branch analysis according to the independent migration evaluation value, and constructing a metastasis branch path distribution diagram; extracting an immunohistochemical staining image of a patient to obtain immune cell space distribution data; and performing multi-region lymphatic metastasis simulation and metastasis risk situation prediction based on the immune cell spatial distribution data and the metastasis branch path distribution diagram to obtain a final metastasis risk assessment result. According to the method, the lymph node metastasis risk is accurately and efficiently analyzed, and the credibility of a pathological analysis result is improved.

The present invention relates to a novel lipid compound for tissue-specific delivery, and a lipid nano-particle (LNP) composition comprising the same, the lipid nano-particle comprising a modified lipid compound as a component, according to the present invention, internal active substances are selectively delivered into cells of specific tissues such as lymph nodes, spleen, retina, cancer, brain, liver and the like in vivo, thereby preventing side effects and safely exhibiting a desired level of effect. The tissue-specific non-viral LNP delivery vectors can be effectively used for prevention of infectious diseases and treatment of rare and refractory (hereditary) diseases (diseases which are effectively and selectively delivered to in-vivo targeted sites, such as macular degeneration, diabetic retinal degeneration, hereditary retinal degeneration, cancer, cerebral diseases, liver diseases and the like).

This invention discloses a method for constructing a biological model of tertiary lymphoid structures, which can rapidly and efficiently induce the generation of in situ TLS, without relying on specific tissues or organs, and without the need for complex and expensive preparation processes such as cell editing and transgenic mice. It can generate stable, mature, and long-lasting TLS structures within 2 to 3 weeks, and has the characteristics of short cycle, low cost, simple operation, reproducibility, safety and effectiveness.

An isolated cell having a central memory T-lymphocyte (Tcm) phenotype, the cell being tolerance-inducing cell and capable of homing to the lymph nodes following transplantation, the cell being transduced to express a cell surface receptor comprising a T cell receptor signaling module is described. Methods of generating same and using same are also described.

The invention discloses a lymph node detection method, system and equipment based on anatomical environment perception and a medium, belongs to the technical field of artificial intelligence medicine, and aims to solve the technical problem of low accuracy of lymph node detection. Comprising the steps of acquiring samples and label data, constructing and training a lymph node detection model, and performing real-time lymph node detection. The lymph node detection model comprises a feature extraction network, a cross-slice attention sub-module guided by a central slice, a three-dimensional channel and space significance enhancement sub-module, a hybrid encoder and Transform decoder sub-module and a detection head sub-module; an auxiliary anatomy environment sensing module is adopted to constrain prototype consistency and difficult negative samples; the auxiliary anatomy environment perception module comprises an ROI-level double-view data enhancement sub-module, a prototype consistency constraint sub-module and a difficult negative sample comparison sub-module. The method can enhance the distinguishing ability of the model to the lymph node and the complex anatomical environment where the model is located, and the lymph node detection accuracy is higher.

The invention belongs to the technical field of biomedical functional dyes and probes, and relates to a fluorescent probe, in particular to a cyclodextrin-based near-infrared fluorescent probe, which is a conjugate formed by coating a fluorescent molecule with cyclodextrin. According to the near-infrared fluorescent probe disclosed by the invention, cyclodextrin is nested on a carbon chain of a fluorescent molecule, so that the water solubility, histocompatibility and safety are improved, the fluorescence lifetime is prolonged, the light stability is improved, and rapid transmission and response of ureters, lymphatic vessels and lymph nodes in vivo are realized; the method can be used for NIR-I and NIR-II imaging; a near-infrared fluorescence probe with targeting property can be formed by marking various tumor targeting molecules, so that accurate targeting fluorescence imaging of different types of tumors such as urogenital tumors, head and neck cancers, breast cancers and cervical cancers and metastatic lymph nodes in NIR-I and NIR-II windows is realized, and the near-infrared fluorescence probe can be applied to fluorescence imaging navigation in clinical operations.

This invention discloses an automatic segmentation and scoring method and system for FDG PET-CT lesions in lymphoma, belonging to the field of medical image analysis technology. It aims to improve the segmentation accuracy of lymphoma lesions and the objectivity of the Deauville score. First, standardized uptake values ​​are calculated for FDG PET images, and lymph node morphological features are extracted from CT images based on multi-scale Hessian enhancement filtering to construct a dual-modality PET-CT image pair. Then, a dual-channel depth network is used to extract anatomical structural features and metabolic distribution features respectively. Cross-modal gating fusion is used to suppress physiological uptake interference, outputting preliminary lesion segmentation results. Next, three-dimensional connected component analysis is performed on the segmentation mask, and metabolic heterogeneity index is extracted by combining kurtosis and Haar wavelet multi-scale energy. A graph attention network is used to identify key lesions. Finally, the ratio of key lesions to standardized liver uptake values ​​is combined with the metabolic heterogeneity index to correct the Deauville score, achieving automation from lesion detection to treatment efficacy evaluation.

The invention discloses a thyroid cancer and lymph node accurate positioning system based on three-dimensional imaging, which relates to the technical field of medical image modeling, and comprises the following steps: acquiring two-dimensional images of a cross section and a coronal plane, preprocessing the acquired two-dimensional images, extracting image features of the preprocessed two-dimensional images, and constructing a three-dimensional model based on the image features. Evaluating and adjusting the constructed three-dimensional model, displaying the adjusted three-dimensional model by using a visual tool and providing an interaction tool, generating an electronic diagnosis report based on a three-dimensional model positioning result, storing the generated electronic diagnosis report to a hospital internal database, and performing encryption measures on the access authority of the hospital internal database. According to the thyroid gland and lymph gland positioning method, feature processing is performed based on the preprocessed image, the three-dimensional model is constructed based on the feature processing result, and the adjusted three-dimensional model is displayed by using the visual tool, so that the accuracy of thyroid gland and lymph gland positioning is improved, and the complexity of an operation and the risk of lymph gland omission are reduced.

The invention provides a preparation method and application of a nitrogen-containing heterocyclic micromolecule modified polyethyleneimine polymer derivative. Compared with a polyethyleneimine polymer, the nitrogen heterocyclic ring micromolecule modified polyethyleneimine polymer derivative provided by the invention has immunostimulatory activity. Besides, the polymer derivative material provided by the invention can be simply mixed with a protein antigen to form nanoparticles, so that efficient loading of the protein antigen is realized, the delivery efficiency of the protein antigen to lymph nodes is improved, and the polymer derivative material can be used for preparing a novel nano vaccine preparation.

This invention provides a method, device, and equipment for preoperative risk stratification assessment of endometrial cancer. It involves segmenting and annotating preoperative ultrasound images to extract radiomics feature vectors, and simultaneously performing structured encoding and mapping of pathological biopsy data to generate pathological feature vectors. These two feature vectors are then input into a fusion network containing a cross-modal attention gating module. This gating module automatically generates dynamic weight vectors based on the consistency between the image and pathological features. The two feature vectors are then weighted and modulated separately before being concatenated to obtain a joint feature representation. This automatically reduces the weight of the lower-confidence modality and amplifies the weight of the higher-confidence modality when their assessment conclusions are inconsistent. Finally, the joint feature representation is input into a multi-task prediction network to output the probability of myometrial invasion depth and the probability of lymph node metastasis risk, thereby generating preoperative risk stratification results and surgical plan recommendations.

A deployment system for accurate placement of localization tags within biological tissue is disclosed. The system comprises a catheter hub with sheath, a stylet, and a loader. The catheter hub provides an interface for system components, while the flexible sheath allows navigation to target sites. The stylet, operable in multiple positions, guides the sheath and assists in tag deployment. The loader houses the localization tag and acts as a safety spacer during deployment. The system is compatible with endoscopes and various imaging modalities, offering improved accuracy and efficiency over traditional wire localization techniques. The localization tags may be passive or active, with potential for advanced functionalities such as biosensing or drug delivery. This minimally invasive system finds applications in tumor marking, lymph node localization, and targeted therapy delivery across multiple medical specialties.

The invention belongs to the technical field of biological medicines, and particularly discloses a pH and ultrasound double-response type oncolytic microorganism as well as a preparation method and application thereof. On the basis of a tumor targeting platform, a mild thermal response gene loop expression GM-CSF and a surface-coated oncolytic microbial system are integrated, chemotherapeutic drugs are released in a tumor core area, and immunogenic cell death is induced. A thermal response loop is accurately activated through low-intensity focused ultrasound, and engineering bacteria are promoted to express GM-CSF and secrete a large amount of mannose modified OMVs. Due to the nanometer size and mannose targeting of the OMVs, the OMVs are efficiently enriched in lymph nodes, the OMVs are reprogrammed into an immune activation state from an immune tolerance state, and the OMVs and ICD cooperate to promote dendritic cell maturation, tumor antigen presentation and activation of tumor killer T cells, so that a remarkable and powerful treatment effect is achieved in various tumor models, and the application prospect is wide. A new strategy is provided for remodeling the lymph node immune microenvironment and enhancing the anti-tumor immune response.

The invention belongs to the technical field of biology, and discloses a preparation method of an intestinal plexus patch, which comprises the following steps: killing an intestinal tract donor, fixing intestinal tissue segments of duodenum, jejunum, ileum or colon by using paraformaldehyde, and dehydrating to obtain dehydrated intestinal tissue segments; washing the dehydrated intestinal tissue section and removing mesentery, fat and Pari's lymph nodes attached to the jejunum, cutting an intestinal mass of about 4-6mm perpendicular to the intestinal tissue section, flatly laying the intestinal mass on a glass slide, dropwise adding a PBS (Phosphate Buffer Solution) of Triton X-100, fixing the intestinal mass by using a smooth surface of a pair of tweezers, stripping muscle tissues along the annular muscle direction, and drying the intestinal mass in a drying oven. The intestinal slices with intermuscular nerve plexus are obtained; finally, the intestinal piece with the intermuscular nerve plexus is cleaned, then fluorescent staining and piece sealing are conducted, and the mouse intestinal nerve plexus patch is obtained. The preparation method of the mouse intestinal nerve plexus patch has the advantages of being convenient, rapid and the like.

The invention discloses an application of an LAMP1 inhibitor in preparation of a medicine for preventing and / or treating esophageal cancer. Specifically, the application value of the LAMP1 inhibitor Parishin C in esophageal cancer treatment is found and verified for the first time, invasion and metastasis of esophageal squamous carcinoma cells and in-vivo lymph node metastasis can be remarkably inhibited by targeting LAMP1 protein and downstream signal channels of the LAMP1 protein and Parishin C, and a new drug choice is provided for metastatic esophageal cancer lacking effective treatment means clinically.

The application provides a hyaluronic acid artificial lymph node sustained-release nano DNA vaccine for enhancing cancer prevention effect and a preparation method thereof, and the preparation raw materials comprise a plasmid, 4-(bromomethyl)phenyl boronic acid modified linear polyethylene imine, a cell colony stimulating factor, a sulfhydryl modified hyaluronic acid and a four-arm-maleimide grafted polyethylene glycol. The nano DNA vaccine can recruit a large number of immune cells to form a hyaluronic acid artificial lymph node after subcutaneous administration, and inhibit the generation of tumors by continuously outputting antigen presenting cells and antigen specific T cells. In addition, the hyaluronic acid artificial lymph node can also release the nano DNA vaccine, prolong the vaccine stimulation time, improve the problem of weak immunogenicity of the existing DNA vaccine, and also solve the problem of biocompatibility. The nano DNA vaccine has the advantages of easy preparation, easy storage, low price and the like.

The present invention relates to glycosylate HIV timer nanoparticles fused to self-assembling ferritin proteins which may be utilized as immunogens to enhance trafficking to lymph nodes and germinal centers and to heighten immune responses.

The invention discloses a tumor detection marker and application thereof in medicine preparation, and belongs to the technical field of biomedicine. Clinical sample qRT-PCR (quantitative real-time polymerase chain reaction) shows that ENST00000412203.1 is obviously up-regulated in breast cancer tissues, the area (AUC) under the ROC curve reaches 0.9822, and the diagnosis efficiency is excellent. In addition, the invention further finds that the expression of the polypeptide in lymph node metastasis tissues is obviously improved, and metastasis specificity is displayed. Meanwhile, the expression of ENST00000412203.1 is knocked down through siRNA, it is found that proliferation, migration and invasion of breast cancer cells are remarkably inhibited, and therefore the siRNA can be used for preparing targeted therapy drugs for breast cancer and has important clinical application value.

The utility model discloses a lymph node developing tracer agent locating hook, relates to minimally invasive surgery auxiliary instrument technical field, including holding cylinder, puncture needle, anchor needle claw, the puncture needle is fixed in the centre of holding cylinder tail end, the push tube is inserted in the puncture needle, the push tube tail end is fixed with the connector, anchor needle claw is fixed in the anchor needle claw, the anchor needle claw is fixed in the anchor needle claw, and the anchor needle claw is fixed in the anchor needle claw. A clamping-in head is fixedly arranged on the side face of the holding cylinder, and a safety structure for preventing the anchoring needle claw from being accidentally released is arranged at the position, at the clamping-in head, of the holding cylinder. The positioning hook is composed of the four groups of anchoring needle claws, the four groups of anchoring needle claws are in four-claw arc shapes and are made of nickel-titanium memory alloy materials, when the positioning claws are released, lung tissue is not prone to being torn, the safety structure is arranged, and the safety structure can prevent the positioning claws from being accidentally released when puncture of the puncture needle is not finished. Scales are arranged on the outer walls of the puncture needle and the positioning line, so that a doctor is assisted in determining the puncture depth and the release depth under the condition that an external CT instrument is not used.

The application belongs to the technical field of medical detection, and particularly relates to a polysaccharide contrast agent capable of renal metabolism and a preparation method thereof. The polysaccharide contrast agent has a structure as shown in formula (I), a particle size less than or equal to 8 nm, and can be rapidly metabolized through the kidney. The preparation method is to graft a metal macrocyclic ligand to a nano-crosslinked polysaccharide particle, and to prepare through a deprotection and metal ion chelation step. Since the contrast agent has the characteristic of renal metabolism, phagocytosis of the reticuloendothelial system is reduced, in vivo deposition of the contrast agent is reduced, and thus the biological safety is improved. Since a macrocyclic chelating ligand is used, good kinetic stability is achieved, the risk of in vivo metal ion deposition is reduced, and when applied to in vivo imaging, clear imaging of cardiovascular, lymph node, liver and other parts can be achieved. The preparation method is simple and easy to implement, reaction conditions are mild, is conducive to large-scale production and clinical transformation, and has a wide biomedical application prospect.

Provided herein are vaccine compositions for use in the stimulation of broad-spectrum memory B cell expansion in a patient that has been exposed or is at risk of exposure to an infectious agent of unknown etiology. The composition comprises IL-15 or an IL-15 analog and at least two toll-like receptor (TLR) agonists, wherein the agonists are targeted to different members of the TLR family. Further contemplated herein are compositions and methods of stimulating germinal B cell expansion in a patient, the composition comprising IL-15 or an IL-15 analog and a nucleic acid, wherein the nucleic acid encodes at least one immunogenic peptide, and wherein the composition is administered either subcutaneously or directly into a lymph node.

The invention discloses a Toll-like receptor (TLR) agonist / organic photosensitizer protein nano-composite as well as a preparation method and application of the Toll-like receptor (TLR) agonist / organic photosensitizer protein nano-composite. In order to overcome the defects of short blood half-life period, low bioavailability and the like of the existing TLR agonist, the protein nano-composite is obtained by forming a compound in a protein cavity by using the TLR agonist and an organic photosensitizer, and has tumor and lymph node dual-targeting characteristics. The protein nano-composite is simple in preparation method, mild in condition, free of an organic solvent, uniform in particle size and has a pH-responsive drug release behavior, and the protein nano-composite is prepared by a one-step method with water as a solvent. Based on an active targeting mechanism mediated by an albumin binding receptor and an active uptake mechanism of antigen presenting cells to albumin, the nano-composite has tumor and lymph node dual-targeting characteristics, has immunotherapy and light therapy effects under irradiation of near-infrared light, and has a good application prospect from two aspects of short-term quick action and long-term body immunity improvement. Tumor growth is effectively restrained, and tumors are expected to be radically treated.

The invention relates to ionizable amphiphilic Janus dendrimers with different linker lengths. In some embodiments, the different linkers enable delivery of the agent to a specific target of interest (e.g., kidney, heart, lung, liver, lymph nodes, spleen, or any combination thereof). In some embodiments, the invention relates to nanoparticles comprising said ionizable amphiphilic Janus dendrimers. In some embodiments, the invention relates to compositions comprising said ionizable amphiphilic Janus dendrimers. In some aspects, the present invention also relates to methods of inducing an adaptive immune response in a subject comprising administering to the subject an effective amount of said nanoparticle or said composition.

This invention discloses an endoscopic device for thyroid surgery based on fluorescence lifetime and Raman spectroscopy imaging, comprising: a light source assembly, an endoscope probe, a fluorescence lifetime image signal acquisition module, a Raman spectroscopy signal acquisition module, and a color image acquisition module; a host unit, including a control unit and an image processing unit; and a display for displaying an image fused with the Raman spectroscopy signal, the fluorescence lifetime image signal of cancerous tissue, and the color image, as well as an image fused with the Raman spectroscopy signal and the color image. This invention utilizes Raman spectroscopy to visualize the autofluorescence of the parathyroid glands, enabling precise localization of the parathyroid glands. Raman spectroscopy can also locate regional lymph nodes and adipose tissue in the central thyroid region, and combined with fluorescence lifetime imaging, it can provide the conformational state of fluorophores in the neck tissues, marking cancerous tissues and providing real-time optical auxiliary localization for differentiating various anatomical tissues during thyroid endoscopic surgery.

The invention relates to a tumor antigen-loaded CD83 positive dendritic cell preparation. The tumor antigen-loaded CD83 positive dendritic cell preparation is prepared by the following steps: (1) inducing immature DCs; (2) antigen loading and specific maturation stimulation: carrying out loading according to the selected antigen type; if a polypeptide antigen is used, resuspending cells in a complete culture medium containing rmTNF-alpha and polypeptide; if the tumor cell lysate is used, resuspending the cells in a complete culture medium containing rmTNF-alpha and the lysate; (3) screening and obtaining a CD83 positive cell population; and (4) preparing active ingredients of the medicine. The invention also provides a preparation method and application of the tumor antigen loaded CD83 positive dendritic cell preparation. According to the invention, the specific CD8T cells aiming at the loaded tumor antigen can be effectively activated and amplified; the tumor drainage lymph nodes are collected preferentially, and tumor antigen specific CD8T cells are activated to play a killing role; while the anti-tumor effect is achieved, the immune tolerance of a maternal-fetal interface in a pregnancy state is not damaged, and normal development and survival of embryos are guaranteed.

The present disclosure provides a microemulsion-based vaccine delivery system, and further provides a preparation method and an application thereof. Using the microemulsion absorbing a series of metal ion compounds, and adding an antigen in a preparation process, antigen entrapment can be realized and a stable vaccine preparation is obtained. The prepared vaccine can effectively be taken up by an antigen-presenting cell and effectively delivered to lymph nodes to induce an antigen-specific immune response, and the same has a wide application prospect.

The application discloses albumin site-directed modification lipid nanoparticles and preparation and application thereof, and relates to a kind of albumin site-directed modification lipid nanoparticles, which is prepared by synthesizing functionalized low molecular weight polyethylene glycol (PEG) lipid compound-maleimide derivative, together with the nucleic acid delivery lipid composition material usually used, to prepare nucleic acid-loaded lipid nanoparticles, then make the maleimide on its surface couple with the thiol group of albumin, to prepare the lipid nanoparticles with albumin site-directed modification on the surface.The application reduces the immunogenicity generated by the existing mRNA-loaded lipid nanoparticles outer layer covered PEG to the body, improves the related immune organ targeting of the nanoparticles, effectively delivers mRNA to lymph nodes to induce anti-tumor effect, and lays a foundation for further developing it as a new type of vaccine that can activate the immune system to produce anti-tumor effect.

The invention discloses a composition for preventing and treating oral diseases or conditions, a preparation method and application thereof. The external vesicles based on oral pathogenic bacteria are rich in nano-scale vesicles of immune activation and defense factors aiming at periodontitis, and after the external vesicles enter a body through oral mucosa administration, the external vesicles are passively targeted into lymph nodes related to the oral mucosa to induce mucosa immune specific antibody expression, so that the immune defense capacity to the pathogenic bacteria is improved. Preferably, a calcium phosphate shell is coated outside the oral bacterial outer vesicle, and the calcium phosphate shell can only be dissolved under an acidic condition, so that the engineered vesicle can be degraded after targeting to lymph nodes and being phagocytosed into dendritic cell lysosome, and the inflammatory reaction of a drug delivery area is avoided. The administration form of the sublingual disintegrating tablet can improve the bioavailability of the vaccine while local mucosal administration activates mucosal immunity, and the convenient administration form is also beneficial to the promotion and popularization of the periodontitis vaccine in clinical chair-side diagnosis and treatment or grassroots communities.

The application discloses a lactobacillus rhamnosus capable of significantly increasing the number of enteric neurons and neuroglial cells and an application thereof, and belongs to the field of microorganisms.The lactobacillus rhamnosus CCFM1360 has good activity, certain acid and alkali resistance and adhesion, can significantly increase the number of enteric neurons and neuroglial cells, repair intestinal barrier function, increase the content of SCFAs in colon contents, increase the number of Treg cells in mesenteric lymph nodes, reduce the expression level of inflammatory factors in colon tissues, reduce the intestinal inflammation level, and relieve the weakened intestinal motility disorder.