14 results about "Promoter activity" patented technology

This invention discloses cis-acting elements that enhance the promoter activity of the cotton SWP73B gene and their applications. The invention provides two cis-acting elements that can improve promoter activity, thereby increasing gene transcription levels. These two cis-acting elements are located on the promoter of the cotton boll weight gene GrSWP73B, the sequence of which is SEQ ID NO:1. The cis-acting elements are named GrmotifD and GrmotifE, with their core sequences being CATCA and ATTAA, respectively. The core cis-acting elements GrmotifD and GrmotifE provided by this invention can enhance promoter activity. Vectors containing these cis-acting elements can be used to transform cotton, enabling widespread and efficient expression of the target gene and cultivating new materials with large boll characteristics.

This invention discloses tree fern. AspiMYB5 The application of genes in regulating the biosynthesis of tree fern phenols belongs to the field of genetic engineering technology, which involves regulating the biosynthesis of tree fern phenols within plants. AspiMYB5 The expression level of the gene, thereby regulating the key enzyme gene of tree fern phenol. AspiPKS6 The expression of [a specific enzyme] and the synthesis of tree fern phenol. This invention uses a yeast library screening method to screen genes affecting key enzymes. AspiPKS6 Expressed transcription factors AspiMYB5 The results were verified using yeast single-hybrid technology (Y1H) and electrophoretic mobility variation analysis (EMSA). AspiMYB5 and AspiPKS6 The promoter exhibits direct physical binding in vitro. Dual-luciferase reporter gene assays further demonstrate that... AspiMYB5 and AspiPKS6 When the promoter is co-expressed, the fluorescence signal intensity is significantly reduced, further confirming... AspiMYB5 It can inhibit AspiPKS6 AspiMYB5 Promoter activity. This invention not only improves the theoretical research on the biosynthetic pathway of tree fern phenols, but also provides key target genes and technical support for regulating the content of secondary metabolites in rare plants through genetic improvement.

The present application relates to a novel promoter and a method for producing a target product using same. A polynucleotide according to one embodiment has promoter activity, can be introduced into a microorganism to enhance the expression and activity of a gene operably linked thereto, and can be effectively used for efficiently producing a target product that is affected by the polynucleotide and the gene.

The application discloses a pig beta-defensin-3 gene core promoter and a construction method and application thereof, and belongs to the technical field of genetic engineering. The sequence of the core promoter is shown as SEQ ID No. 1, the core promoter has significant promoter activity in pig small intestinal epithelial cells, and can be regulated by nutrients such as sodium butyrate and glutamine. The application also discloses a construction method for specifically amplifying the core promoter, which comprises the following steps: performing PCR amplification by taking pig small intestinal epithelial cell DNA as a template, taking the upstream primer and the downstream primer shown as SEQ No. 2-SEQ No. 3 as the primer, constructing a pMD-18T-pBD-3-P recombinant plasmid, and taking the recombinant plasmid as a template to amplify a core promoter fragment. The core promoter provides an excellent experimental system for studying the transcriptional regulation mechanism of the pig beta-defensin-3 gene, the nutrient response characteristics provide a new platform for studying the interaction between nutrition and immunity, and the core promoter has a wide application prospect in the field of healthy breeding of livestock and poultry.

The present application relates to the field of molecular biology and genetic engineering technology, and discloses a chicken SLC11A1 gene core promoter and a construction method and application thereof.The sequence of the chicken SLC11A1 gene core promoter is shown in SEQ ID NO:2, and the chicken SLC11A1 gene core promoter has significant promoter activity in a chicken monocyte macrophage cell line HD11.The present application simultaneously discloses a construction method for specifically amplifying the core promoter, and the construction method comprises the following steps: taking a chicken SLC11A1 full-length promoter fragment (SEQ ID NO:1) as a template to perform PCR amplification, and the upper and lower upstream primers are shown in SEQ ID NO:15 and 23 respectively, and a core promoter fragment is obtained by amplification.The core promoter provides an excellent experimental system for studying the transcriptional regulation mechanism of the chicken SLC11A1 gene.

This invention discloses VvENO2 The application of genes in regulating plant fruit development belongs to the field of plant genetic engineering technology. Previous research in this invention found that hydrogen peroxide (H2O2) treatment significantly promotes grape fruit ripening. Transcriptome analysis of H2O2-treated grapes revealed… VvENO2 Gene expression levels decreased. To further clarify... VvENO2 The role of the gene in plant fruit development was investigated through subcellular localization experiments and promoter activity analysis, demonstrating its nucleus location and that its promoter can activate the GUS reporter protein. Furthermore, treatment with ABA and H2O2 enhanced its activity. In addition, transgenic Arabidopsis and transgenic grape were constructed, and phenotypic observations confirmed... VvENO2 Genes negatively regulate the ripening of plant fruits. This invention provides a new regulatory site for breeding early-maturing plant varieties.

This invention discloses the promoter EP11 and related biomaterials and applications, belonging to the field of biotechnology. The technical problem solved by this invention is how to increase the content of L-lysine. The promoter EP11 disclosed in this invention has the sequence shown in SEQ ID No. 1 of the sequence listing. The EP11 promoter is introduced into *Corynebacterium glutamicum*, a bacterium capable of synthesizing L-lysine, thereby driving the expression of genes in the L-lysine synthesis pathway, resulting in recombinant *Corynebacterium glutamicum*. Culturing this recombinant *Corynebacterium glutamicum* yields L-lysine, and the production of L-lysine is increased. This demonstrates that the EP11 promoter of this invention not only possesses promoter activity but can also be used for the production of L-lysine.

The present application relates to the field of plant molecular biology and genetic engineering technology, and particularly relates to a transient expression method of orchid plants and application. The method provided by the present application successfully breaks through the infection barrier of the thick cuticle of orchid plants by optimizing the core parameters of vacuum-assisted Agrobacterium infection, screening the infection strains and optimizing the composition of the infection solution. The high activity of the leaf cells can be maintained for more than 9 days after infection, and the exogenous genes can realize stable and efficient expression. The method provided by the present application is simple to operate, does not require special instruments and equipment, is convenient to obtain materials, is not limited by the flowering season, can be carried out throughout the year, and the complete experimental cycle can be completed within 7 days. The present application effectively solves the long-standing technical problems in the field of genetic transformation of orchid plants, and provides an efficient and convenient gene function transient verification technology platform for the functional genomics research, gene function verification, promoter activity detection and precise molecular breeding of orchid and other orchid plants.

This invention provides a tobacco polyphenol oxidase gene. NtPPO15 This invention relates to the inhibition of tobacco browning and the improvement of tobacco's ripening and roasting resistance, and its application in these areas. Through experimental screening, key genes related to tobacco browning and roasting resistance were identified. NtPPO15 Further investigation was conducted by constructing a GUS staining vector. NtPPO15 This invention investigated promoter activity and gene expression sites, and further explored gene function through gene editing and overexpression. The invention experimentally demonstrates the benefits provided by this invention. NtPPO15 These genes are key genes involved in browning during the ripening and curing process of tobacco, and they regulate... NtPPO15 NtPPO15 The expression level of genes can control the browning of tobacco, improve the ripening and roasting resistance of tobacco, improve the quality of tobacco products, reduce the number of harvests, greatly improve economic benefits, and can also be used to screen tobacco varieties with excellent quality. Therefore, it has good and broad market application prospects.

The present invention belongs to the field of biotechnology. Provided are promoter EP11, and a related biological material thereof and the use thereof. The technical problem to be solved is how to increase the content of L-lysine. The provided promoter EP11 has a sequence of SEQ ID No. 1 in the sequence listing. The EP11 promoter is introduced into Corynebacterium glutamicum capable of synthesizing L-lysine, such that the EP11 promoter drives the expression of genes in the L-lysine synthesis pathway, thereby obtaining recombinant Corynebacterium glutamicum. The recombinant Corynebacterium glutamicum is cultured to obtain L-lysine, and the yield of the L-lysine is increased. It is indicated that the provided EP11 promoter not only has a promoter activity, but can also be used for producing L-lysine.

  42      Abstract  The present invention is directed to nucleic acid molecules which encode novel luciferases, functional fragments thereof, homologs and mutants, as well as to proteins encoded by said nucleic acids. The nucleic acid molecules of interest are isolated from fungi or obtained by genetic engineering methods. Also, host cells, stable cell lines and transgenic organisms comprising said nucleic acid molecules are provided. In addition, antibodies specific to the proteins of the present invention are provided. Said proteins and nucleic acids are used in many applications and methods, in particular, in labelling organisms, cells, cellular organelles, or proteins. Also, said protein and nucleotide compositions are used in methods for detecting protein-protein interactions, for testing promoter activity under various conditions. Finally, provided are kits for the use of proteins and nucleic acids of the present invention in the diversity of methods and applications. Abstract The present invention is directed to nucleic acid molecules which encode novel luciferases, functional fragments thereof, homologs and mutants, as well as to proteins encoded by said nucleic acids. The nucleic acid molecules of interest are isolated from fungi or obtained by genetic engineering methods. Also, host cells, stable cell lines and transgenic organisms comprising said nucleic acid molecules are provided. In addition, antibodies specific to the proteins of the present invention are provided. Said proteins and nucleic acids are used in many applications and methods, in particular, in labelling organisms, cells, cellular organelles, or proteins. Also, said protein and nucleotide compositions are used in methods for detecting protein-protein interactions, for testing promoter activity under various conditions. Finally, provided are kits for the use of proteins and nucleic acids of the present invention in the diversity of methods and applications. 42 20 24 20 33 78 21 M ay 2 02 4 A b s t r a c t 2 0 2 4 2 0 3 3 7 8 2 1 M a y 2 0 2 4

This invention belongs to the field of biology, specifically relating to the application of the rubber tree HbEIN3-1 gene in regulating the biosynthesis of natural rubber. The HbEIN3-1 gene described in this invention binds to the promoter of the HbCPT gene, enhancing the promoter activity of the HbCPT gene. This indicates that HbEIN3-1 participates in the regulation of HbCPT expression at the transcriptional level, and that the expression level of HbCPT is positively correlated with the expression level of HbEIN3-1, thus directly participating in the regulation of natural rubber biosynthesis. This invention elucidates the molecular mechanism by which HbEIN3-1 regulates natural rubber biosynthesis, providing a new regulatory gene for increasing natural rubber yield.

The present application relates to a novel promoter and a method for producing a target product using the same. The polynucleotide of the present application has promoter activity and can effectively regulate the expression and activity of a target gene in a microorganism and can be effectively used for efficient production of a target product.

This invention belongs to the fields of plant molecular biology and genetic engineering technology, specifically relating to negative regulation. ZmSWEET4b The application of genes or their encoded proteins in enhancing resistance to maize sheath blight. This invention was validated using EMS-induced variants and CRISPR mutants. ZmSWEET4b Negative regulation of maize resistance to sheath blight. ZmMYB20 inhibition ZmSWEET4b Gene promoter activity, ZmbHLH164 Promote ZmSWEET4b Gene promoter activity, when both transcription factors are present ZmSWEET4b Promoter region activity was significantly lower than in the wild type. Overexpression ZmMYB20 Or co-expression ZmMYB20 and ZmbHLH164 Able to inhibit ZmSWEET4b Gene promoter activity, thereby inhibiting ZmSWEET4b The expression of genes or their encoded proteins can enhance resistance to corn sheath blight.