157 results about "Brassinolide" patented technology

The invention provides an anti-premature senility management method for closely-planted double-layer truncated cone macadamia nut trees, and belongs to the technical field of fruit tree cultivation. The premature senility prevention management method comprises layered nutrition supply and flower and fruit protection management. The layered nutrition supply comprises the following steps: deeply applying a calcium magnesium phosphate fertilizer to the lower layer of soil greater than 60cm, and covering a root controller; organic fertilizers and biological agents are applied to 30-60 cm soil of the middle layer; a macroelement, humic acid and seaweed extract water-soluble fertilizer is applied to the 0-30 cm soil of the upper layer; the flower and fruit protection management comprises the steps that ABA and paclobutrazol are sprayed in the flower bud differentiation period; brassinolide and fluid boron are sprayed in the full-bloom stage; spraying forchlorfenuron and amino acid in a young fruit stage; nAA and monopotassium phosphate are sprayed in the fruit swelling period. Layered nutrition supply and flower and fruit protection management are carried out on the densely-planted double-layer truncated cone macadamia nut trees, premature senility of the densely-planted macadamia nut trees is prevented, branch development of the lower layer is promoted, the tree vigor is balanced, and the high-yield level is maintained.

The invention discloses a potassium fulvate chelated macroelement water-soluble fertilizer with stress resistance and growth promotion functions, and relates to the technical field of water-soluble fertilizers, the potassium fulvate chelated macroelement water-soluble fertilizer comprises the following raw materials by weight: 167-210 parts of mineral source potassium fulvate, 450-460 parts of potassium nitrate, 300-310 parts of urea, 150-160 parts of potassium dihydrogen phosphate, 30-40 parts of potassium sulfate, 15-25 parts of EDTA-disodium, and 5-15 parts of citric acid. 0.5 to 1.5 parts of brassinolide and 4 to 6 parts of polyaspartic acid; according to the potassium fulvate chelated macroelement water-soluble fertilizer with the stress-resistant and growth-promoting functions, through chelation cooperation of potassium fulvate and nitrogen, phosphorus and potassium, the solubility of the chelated phosphorus element in water is increased, and the chelated phosphorus element can be more uniformly distributed in soil; by adopting an EDTA-disodium and citric acid composite chelating system, volatilization and loss of nitrogen elements are reduced, the problem of precipitation of calcium, magnesium and phosphorus is solved, and the soil conditioner is suitable for hard water areas and especially has outstanding performance in saline-alkali soil and arid areas.

The invention discloses a water and fertilizer management method for transplanted high-yield indica type two-line hybrid rice, and belongs to the technical field of rice cultivation. Comprising the following steps: (1) applying citric acid chelated calcium magnesium phosphate fertilizer to soil before transplanting; (2) applying coated controlled-release urea and a silicon-zinc fertilizer on the third day after transplanting; (3) when the tiller number reaches 2650,000-2750,000 plants / hm < 2 >, starting intermittent light field drying for three times, and deeply applying potassium magnesium sulphate before field drying; (4) laterally and deeply applying urea and a boron-humic acid chelate when tips of two inverted leaves are exposed; (5) spraying an aqueous solution in a full heading stage, wherein the aqueous solution comprises monopotassium phosphate, chelated zinc and brassinolide. Through staged precise fertilization, special chelating / controlled release fertilizer and water and fertilizer-field drying cooperation, the method has the advantages that the fertilizer utilization rate is increased, the nutritional requirements of rice in each growth period are precisely matched, and water and fertilizer cooperative management is optimized.

The invention relates to a crop drought-resistant agent, a method for improving crop drought resistance and / or promoting crop growth and development and application of the crop drought-resistant agent, and belongs to the technical field of agricultural production. The crop drought-resistant agent is prepared from the following components: arginine, chitosan, salicylic acid, diethyl aminoethyl hexanoate, betaine, brassinolide and water. The crop drought-resistant agent can promote normal growth and development of crops under drought conditions, improve plant biomass accumulation and cell antioxidant enzyme activity and reduce toxicity of ROS to the plants, so that adaptability, tolerance and resistance of the crops to drought stress are improved, and the crop drought-resistant agent has the advantages of being good in drought-resistant effect, high in stability and high in safety.

The invention discloses a special growth regulator for pepper based on compounding of propionyl brassinolide and amino-oligosaccharin and application of the special growth regulator, and belongs to the technical field of agricultural biology. According to the regulating agent, 0.0008 to 0.0012 percent of propionyl brassinolide and 2.95 to 3.05 percent of amino-oligosaccharin are accurately matched with each other. The formula breaks through the contradiction between the root promotion requirement of high-dose amino-oligosaccharin and the safety threshold of low-dose brassinolide, and overcomes the phytotoxicity risk of the traditional dosage form. The regulator prepared by the invention can enhance the growth vigor of the pepper, improve the product and quality of the pepper, and also can effectively prevent viral diseases of the pepper.

The invention belongs to the field of fertilizers, and particularly relates to a foliar fertilizer with high amino acid synthesis efficiency and a preparation method thereof.The preparation method comprises the steps that spermidine is taken to be subjected to formic acid esterification modification, and formic acid esterification spermidine is obtained; the preparation method comprises the following steps: hydrolyzing 2, 4-epibrassinolide to obtain carboxylated brassinolide; under the action of Tween, carboxylated brassinolide and formic acid esterified spermidine are combined to form a fertilizer base full of emulsified liquid drops; and adding other nutrients to obtain the foliar fertilizer. The leaf fertilizer for increasing the amino acid content of rice can increase the content of amino acids, especially lysine, in grains on the premise of increasing the yield of rice, and nitrogen absorption and utilization of the rice grains are improved.

The invention relates to the technical field of biology, in particular to application of brassinolide in increasing the yield of lettuce latex. According to the invention, brassinolide is adopted as an active component and acts on lettuce plants, so that the yield of lettuce latex can be remarkably increased, and meanwhile, the contents of sucrose and isopentenyl pyrophosphate in lettuce are further remarkably increased. Compared with indoleacetic acid, the brassinolide provided by the invention is more suitable for improving the gum production performance of the plant lettuce, and the brassinolide and the indoleacetic acid are high in adaptability, so that a more excellent technical effect is obtained. In the use process, the use method of brassinolide is simple, the use amount is small, the function of remarkably improving the yield of lettuce latex, sucrose and isopentenyl pyrophosphate can be achieved by adopting brassinolide with low concentration, the cost is low, the effect is good, no new toxic or side effect is caused to the environment, and the brassinolide is suitable for application and popularization.

A method for improving the germination rate and seedling growth vigor of scutellaria baicalensis seeds in a saline-alkali soil environment comprises the following steps: (1) preparing a seed soaking solution, namely preparing a quaternary plant growth regulator combined 6-BA + GA3 + NAA + brassin seed soaking solution; (2) preparing seed dressing mother liquor, namely preparing ternary seed dressing mother liquor of No.6 seed dressing mother liquor, planting elements and pyroligneous liquor; (3) seed soaking: soaking the seeds by using a quaternary seed soaking solution of 6-BA + GA3 + NAA + brassin; (4) airing; (5) seed dressing: carrying out secondary coating by using ternary seed dressing mother liquor of No. 6 seed dressing mother liquor, planting elements and pyroligneous liquor; and (6) sowing: sowing the seeds treated in the step (5) in a matrix containing 0.4% salt stress, and performing conventional culture. Compared with a traditional planting method, the germination rate and seedling growth vigor of the scutellaria baicalensis seeds treated through the method are greatly increased, the germination rate basically reaches 100%, the germination potential is 90%, and the effectiveness and advancement of the method are fully proved.

The invention relates to the technical field of agriculture, and particularly discloses a cherry tomato whole growth period fertilization method, which comprises the following steps: 10-15 days before field planting, deeply ploughing soil for 30-35 cm, exposing for 3-5 days, adjusting the pH value of the soil to 6.0-7.0, and adjusting the EC value to be less than 1.2 mS / cm; 600-800 kg / mu of carbon enzyme microbial fertilizer and 50-60 kg / mu of compound fertilizer are applied to the upper layer; 150-200 kg / mu of carbon energy soybean organic fertilizer and 100-120 kg / mu of silicon-calcium-magnesium fertilizer are applied to 0-20 cm of the middle layer; applying cattle and sheep manure of which the total amount is greater than or equal to 2000 kg / mu and fermented soybean meal of which the amount is 200-250 kg / mu to 20-40 cm of After fertilization, 300 times of EM bacteria liquid is immediately sprayed; according to the method, layered fertilization is combined with the microbial agent to activate soil activity, a long-acting nutrition library is constructed in a base fertilizer stage, and drip irrigation and foliage topdressing schemes at key nodes in a growth period are matched, so that the absorption efficiency of the cherry tomatoes on nitrogen, phosphorus, potassium and medium trace elements is remarkably improved; through fulvic acid, brassinolide and other substances, crop physiological status is rapidly adjusted, stress of high temperature, cloudy and rainy days and the like is effectively resisted, and fruit development continuity is guaranteed.

The invention discloses a management method for improving cold resistance and drought resistance of macadamia nuts and increasing flowering quantity, which combines a four-stage dynamic management mode of soil improvement, cold resistance induction, drought resistance and tip control and flowering phase quality improvement, applies an organic fertilizer, a water-retaining agent and a composite slow-release fertilizer, induces root development, slowly releases the fertility of a chemical fertilizer, prolongs the action time of the chemical fertilizer, and improves the yield of the macadamia nuts. Brassinolide and potassium fulvate are compounded to activate cold-resistant gene expression of macadamia nut plants and improve cell membrane stability, so that cold resistance of the macadamia nut plants is improved, melatonin and methyl jasmonate perform double shoot control, an ABA signal channel of the plants is triggered, drought resistance of the plants is improved, flower bud differentiation is promoted, and growth of the macadamia nut plants is promoted. The synergistic effect of alginate oligosaccharide and gamma-aminobutyric acid enhances the elongation ability of a pollen tube, chemical agent stimulation is replaced by biological agent stimulation, the utilization rate of chemical agents is reduced, then the negative influence of the chemical agents on soil is reduced, and finally the fruit setting rate and the sustainable utilization of the soil are improved.

The invention discloses a special-effect foliar fertilizer for improving cold resistance of durian seedlings as well as a preparation method and application of the special-effect foliar fertilizer, and belongs to the technical field of durian planting. The special-effect foliar fertilizer prepared by the invention is prepared from the following raw materials in parts by weight: 2 to 5 parts of fulvic acid, 10 to 20 parts of antifreeze agent, 0.5 to 2 parts of pine needle extract, 3 to 8 parts of alginate oligosaccharide, 0.3 to 0.8 part of 14-hydroxybrassinosterol, 0.2 to 0.5 part of 0.01 to 0.1 percent v / v polysorbate-20, 2 to 4 parts of chitosan, 1 to 3 parts of trehalose, 0.1 to 1 part of brassinolide, 0.1 to 0.3 part of salicylic acid and 6 to 10 parts of glycerol. The special-effect foliar fertilizer is simple to prepare, safe overwintering of durian seedlings at the low temperature of 4-15 DEG C can be effectively guaranteed, and the problems of dead seedlings and runt seedlings caused by low temperature are remarkably solved. The durian seedling culture medium is safe and environment-friendly in components, is beneficial to reduction of environmental pollution and pesticide residues, provides rich nutrition for breeding and growth of durian seedlings, and has remarkable application potential for promoting development of the durian industry.

The invention provides an application of an XTH gene in promotion of grape fruit hardness formation by brassinolide, brassinolide (BR) is adopted for treatment in a grape color changing period, it is found that the XTH gene can mediate synthesis of hemicellulose in grape fruits so as to significantly improve the hardness of the grape fruits, and through whole genome correlation analysis and transcriptomics analysis, the XTH gene can be used for promoting the hardness formation of the grape fruits. The two target genes VvXTH25 and VvXTH31 capable of regulating and controlling the hardness of the grape fruits are found, key genes and mechanisms for regulating and controlling the hardness of the grape fruits are defined, the hardness of the fruits is regulated and controlled by regulating and controlling the content of hemicelluloses, and the VvXTH25 and VvXTH31 have the effects of providing a basis and a means for regulating and controlling the texture of the grape fruits and improving the storage and transportation resistance; a brand-new way is provided for really regulating and controlling the hardness of the grape fruits.

The invention discloses a method for adjusting the pore opening degree of Shanghai green, and belongs to the technical field of plant detection. Shanghai leaves are placed in 20mL of open liquid containing (1) 0.5% potassium chloride and 100 [mu] M / L calcium chloride or (2) 0.5% potassium chloride and 50 [mu] M / L brassinolide or (3) 0.5% potassium chloride and 10 [mu] M / L cytokinin or (4) 0.5% potassium chloride and 5 [mu] M / L gibberellin or (5) 0.5% potassium chloride and 100 [mu] M / L methyl jasmonate, culture is performed under illumination, and the illumination time is set to be 5h, so that the pore opening degree of Shanghai is remarkably increased. Compared with a control group (containing 0.5% of potassium chloride), the pore openness of the treatment is respectively increased by 33.5%, 43.5%, 43.4%, 27.1% and 70.2%. The method disclosed by the invention lays a technical foundation for rapidly screening out the exogenous environmental stimulation factor (environical stimuli) capable of increasing the opening degree of the pore.

The invention discloses a multifunctional water-soluble fertilizer containing S-propionyl lactone and a wetting penetrant and a preparation method of the multifunctional water-soluble fertilizer, and relates to the technical field of agricultural fertilizers. The invention relates to a multifunctional water-soluble fertilizer containing S-propionyl brassinolide and a wetting penetrant. The multifunctional water-soluble fertilizer is prepared from the following components in parts by mass: 0.01 to 0.05 part of S-propionyl brassinolide, 0.5 to 3 parts of wetting penetrant, 30 to 35 parts of macroelement fertilizer, 1 to 5 parts of medium element fertilizer, 0.05 to 0.1 part of microelement fertilizer, 5 to 10 parts of dispersing agent, 0.5 to 1 part of antioxidant and 40 to 50 parts of water. Through the synergistic effect of the S-propionyl lactone and the wetting penetrant, the resistance of plants to diseases is enhanced, the disease infection rate is reduced, and the overall health level of the plants is improved. The special antioxidant is adopted, and the optimized preparation process is matched, so that the fertilizer is not easy to precipitate, layer and the like in the storage process, and the long-term stability of the fertilizer is ensured.

The invention belongs to the field of rambutan cultivation, and particularly relates to a special compound fertilizer for rambutan and application thereof. According to the special compound fertilizer for the rambutan, disclosed by the invention, the millettia speciosa champ residues, the bagasse, the mimosa pudica and the fish meal are used as substrates, bacillus velezensis HAB-2 is added for fermentation to prepare an extract, and then fulvic acid, brassinolide and naphthylacetic acid are added to finally prepare the compound fertilizer, so that the growth of rambutan seedlings can be remarkably promoted; on the premise that healthy growth of rambutan seedlings is guaranteed, the seedling raising period of the rambutan is shortened, and the method has important promoting significance on cultivation of the rambutan.

The invention discloses a preparation method of a prevention and control agent for winter low-temperature freezing injury of walnuts. The prevention and control agent is prepared from the following active ingredients in percentage by mass: 0.2 part of agricultural chitosan oligosaccharide, 1 part of carboxymethyl cellulose, 7.8 parts of jasmonic acid methyl ester, 0.3 part of monopotassium phosphate, 0.1 part of compound amino acid, 0.05 part of brassinolide and 100 parts of water. The method effectively solves the problems that the annual branches of the Xinjiang walnuts in winter are prone to freezing, and the existing prevention and control means are poor in effect, reduces the conditions of branch water loss and drying, tree body weakness and even death, can obviously improve the cold resistance of the annual branches and mixed buds, greatly relieves the freezing injury symptoms after adult trees and young trees are treated, and obviously optimizes physiological indexes.

The application discloses a kind of cultivation methods of konjak, belong to konjak planting technical field, the present application is to the pretreatment konjak seed potato of sterilization in nitrogen and ozone mixed atmosphere steam treatment, not only strengthen the growth potential of konjak seed potato, and fully activated the catalase in seed potato, create excellent intracellular environment for promoting subsequent seed potato germination, heat shock seed potato is soaked in activation liquid and activated, brassinolide, calcium sulfate, ethiofencarb and sodium complex nitrophenol in activation liquid activate the cytokinin oxidase in cell, and then regulate seed potato germination speed and germination quality, effectively solve the problem of low seedling rate of offspring konjak and yield decline.The activation seed potato is sowed in nutrient soil, effectively improves the content of nutrient substance in harvested konjak.

The application discloses a method for increasing the content of polyphenols and GABA in germinated brown rice, and uses brown rice as raw material, and comprises the following steps: screening brown rice with complete kernels, cleaning and disinfecting the brown rice, placing the brown rice into a brassinolide solution, activating relevant enzyme activity by ultrasonic treatment, soaking the brown rice after ultrasonic treatment in the brassinolide solution, spraying an ascorbic acid solution to continue germination, synchronously enriching polyphenols and GABA, and finally, after ultraviolet sterilization of the germinated brown rice, drying the germinated brown rice by hot air. The prepared germinated brown rice has synchronous increase in the content of polyphenols and GABA, and the antioxidant capacity is significantly improved, the germination time is shorter, and the sprout length is moderate.

The invention provides a planting method for promoting early maturing and high yield of spring wheat in Ningxia irrigated areas, and belongs to the technical field of crop regulation and control. The method comprises the following steps: soaking seeds with a seed soaking agent, and then early sowing from February 15th to February 25th; the seed soaking agent is prepared from 5 to 10g / L of calcium chloride, 2 to 3g / L of alginate oligosaccharide, 100 to 200mg / L of proline and 0.05 to 0.12 mg / L of brassinolide. Through the synergistic effect of the calcium chloride, the alginate oligosaccharide, the proline and the brassinolide, the germination rate of the early sowing spring wheat seeds at the low temperature of 5 DEG C is increased to 85% or above, the problem of poor spring wheat seedling emergence caused by early sowing low temperature is solved, and the purpose of early maturing without yield reduction is achieved.

This invention belongs to the field of organic agriculture, specifically a biological agent for promoting wheat photosynthesis and its preparation method. Addressing the problems of existing biological agents having relatively simple components, low absorption and utilization efficiency by wheat ears, and a tendency to reduce nitrate reductase activity, nitrogen utilization, and chlorophyll content in wheat leaves, the following solution is proposed: the biological agent comprises the following raw materials in parts by weight: 50-65 parts of yield-enhancing amine, 15-20 parts of choline chloride, 10-15 parts of diethylaminoethanol hexanoate, 5-10 parts of brassinolide, 3-5 parts of p-chlorophenoxyacetic acid, 1-3 parts of calcium diphenylureasulfonate, 2-5 parts of trace agents, and 2-4 parts of ethylene glycol styraldehyde. The biological agent of this invention can promote better photosynthesis in wheat, enhance nitrate reductase activity, and improve nitrogen utilization.

The invention relates to a macadamia nut flower and fruit retention synergistic method which comprises the following steps: in the early stage of flower bud differentiation, annularly applying slow-release granules to the root of a macadamia nut tree, and spraying fructan emulsion to the crown at the same time; in the early flowering period, a water dispersible sustained release preparation containing microencapsulated compound sodium nitrophenolate is sprayed to tree crowns of macadamia nuts, sodium alginate embedded borax sustained release microsphere suspension liquid is sprayed to leaf surfaces, and a hydrogel complexing agent containing sustained release monopotassium phosphate and amino acid is sprayed to the leaf surfaces; in the full-bloom stage, a composite slow-release emulsion containing liposome-embedded compound sodium nitrophenolate and brassinolide is sprayed to tree crowns of the macadamia nuts, slow-release gel microsphere suspension liquid of borax and amino acid is sprayed to leaf surfaces, and slow-release nano-emulsion containing beta-cyclodextrin-embedded bee wax volatile substances is sprayed to the tree crowns; in the initial fruit setting stage, sulfur-coated slow-release compound fertilizer is applied to the roots of macadamia nut trees, and ionic cross-linked calcium alginate microsphere embedded amino acid slow-release foliar fertilizer is sprayed to the leaf surfaces of the macadamia nut trees.

This invention discloses a stress-resistant and yield-increasing plant growth regulator, its preparation method, and its application, belonging to the field of plant growth regulators. The stress-resistant and yield-increasing plant growth regulator of this invention comprises the following raw materials: *Suaeda salsa* extract, a stress-resistant biostimulant, propionyl brassinolide, styracil, polyaspartic acid, γ-aminobutyric acid, emulsifier, penetrant, co-emulsifier, pH buffer, and deionized water; wherein the stress-resistant biostimulant is perilla lactone. This invention significantly improves the tolerance of wheat to salt-alkali stress by strengthening the antioxidant system and osmotic regulation capacity, ensuring its basic survival and normal physiological functions; and effectively reverses stress-induced growth inhibition through mechanisms such as promoting root development, enhancing photosynthesis, and driving product translocation to grains, thereby achieving a significant yield increase under adverse conditions.

The invention belongs to the technical field of planting, and discloses a hormone and leaf surface boron fertilizer spraying method for improving the fruit setting rate of macadamia ternifolia, and the method comprises the following steps: spraying 0.02% boron fertilizer in the flower bud period of macadamia ternifolia; 2 mg / L 6-BA is sprayed in the full-bloom stage of the macadamia nuts; 0.02% of boron fertilizer and 0.2 ml / L of brassinolide are sprayed from the flowering period of the macadamia nuts to the initial fruit setting period. The 0.02% boron fertilizer is sprayed in the flower bud period of the macadamia nuts, so that the pollen viability and the flower quantity of the macadamia nuts are increased, and the fruit setting probability and the total fruit setting quantity in the later period are increased. According to the method, 2 mg / L 6-BA is sprayed at the full-bloom stage of the macadamia nuts, so that the flower falling rate is reduced, and the fruit setting rate is increased.

The invention relates to the field of agricultural biochemical engineering and plant protection, and particularly discloses a liquid film-forming agent for preventing crops from freezing injury due to late spring coldness and a preparation method of the liquid film-forming agent. The film-forming agent is prepared from carboxymethyl chitosan, sodium carboxymethyl cellulose, kasugamycin, brassinolide, glycol and glycerol mixed dihydric alcohol, Tween 80 and deionized water according to a specific proportion. The preparation method comprises the key processes of step-by-step dissolution, mixing, functional component addition and the like. Organic fusion of physical barrier, ice nucleus inactivation and physiological regulation is realized through multiple action mechanisms of natural polymer composite film forming, biopesticide targeted sterilization, plant hormone induced cold resistance and dihydric alcohol synergistic antifreezing. The product can provide efficient protection at a low temperature of-4 DEG C, significantly reduces the frost damage rate, has the characteristic of 28-day natural rapid degradation, and solves the technical bottlenecks of single function, difficult degradation and poor environmental compatibility of a traditional film agent.

The invention relates to a bagging-free quality-improving and efficiency-improving pesticide for reducing the insect fruit rate of pomes and a spraying method of the bagging-free quality-improving and efficiency-improving pesticide. The quality-improving and efficiency-improving pesticide comprises the following components: 1-100 g / L of pullulan; 0.5 g / L to 2 g / L of EDTA-2NaCa; the nutrient solution is prepared from 0.3 g / L to 5 g / L of L-calcium aspartate, 1 g / L to 3 g / L of D-calcium gluconate, 2 g / L to 4 g / L of sucrose fatty acid ester, 5 g / L to 15 g / L of tea polyphenol, 2 g / L to 6 g / L of gallic acid, 0.5 mg / L to 1.5 mg / L of brassinolide, 2 g / L to 4 g / L of fumed silica, 0.1 g / L to 0.3 g / L of xanthan gum, 1 g / L to 3 g / L of D-sorbitol, 0.1 g / L to 0.5 g / L of an anti-coagulant and water serving as a solvent. After the pesticide is sprayed, the pest prevention and control effect is equal to that of bagging, the cost is reduced by 68%, sunburn and bitter pox diseases are reduced, the chlorophyll SPAD value is increased by 22%, the photosynthetic rate is increased by 18%, and high yield is promoted.

This invention belongs to the field of grape cultivation technology, specifically relating to a fruit enlargement agent for Daqing grapes, its preparation method, and its application. The fruit enlargement agent for Daqing grapes is prepared from an aqueous solution of gibberellin, thiabendazole, and brassinolide. Specifically, the concentration of gibberellin in the fruit enlargement agent is 20 mg / L~25 mg / L, the concentration of thiabendazole is 1.0 mg / L~1.25 mg / L, and the concentration of brassinolide is 0.025 μg / L~0.050 μg / L. The fruit enlargement agent of this invention, combined with fruit thinning treatment, can increase the weight of a single Daqing grape berry by at least 20% while retaining the soluble solids content of the grape.

The invention discloses application of brassinolide in improving resistance of sugarcane to smut. According to the method, brassinolide is sprayed on leaf surfaces, so that the brassinolide can improve the resistance of sugarcane to smut caused by sugarcane smut, the morbidity of sugarcane plants infected by sugarcane smut is remarkably reduced, and the influence of sugarcane smut on the yield and quality of sugarcane is reduced. Therefore, the invention provides application of brassinolide in improving or preparing a product for improving the resistance of sugarcane to smut. The method is beneficial to green control of sugarcane smut, and is simple to operate and good in feasibility.

The invention relates to the technical field of agricultural compositions, in particular to an agricultural composition for controlling vigorous growth and increasing yield of sweet potatoes, the effective components of the composition are olefinic potassium phosphate and brassinolide, and the volume ratio of olefinic potassium phosphate to brassinolide is 2: 0.2; when the composition is used, the composition is mixed with clear water according to the volume ratio of 1: 100, 30 L of the composition is sprayed to each mu of land, foliage spraying is carried out four times in the sweet potato growing season, the concentration of the alkene potassium phosphate is 1%-2% w / v, the concentration of the brassinolide is 0.2% w / v, and the dual purposes of controlling vigorous growth and increasing yield of the sweet potatoes are achieved through the synergistic effect of the alkene potassium phosphate and the brassinolide. The problems of improper application, residual pollution and limited yield increasing effect of a traditional vigorous growth control agent are solved, and a safe, economical and sustainable technical scheme is provided for green and efficient cultivation of sweet potatoes.

The invention discloses a tissue culture detoxification method of Amomum tsao-ko, which comprises the following steps: S1, selecting an Amomum tsao-ko plant which grows robustly, taking a tender stem segment of the Amomum tsao-ko plant as an explant, disinfecting, inoculating into a callus induction culture medium, culturing, and inducing the generation of callus; wherein the callus induction culture medium is prepared from 0.8 mg / L to 1.2 mg / L of MS + 2, 4-D, 0.4 mg / L to 0.6 mg / L of 6-BA and 3 mu mol / L to 5 mu mol / L of chicoric acid; s2, heat treatment: cutting the callus obtained in the step S1 into small pieces, and putting the small pieces into sterile water at 40 + / -2 DEG C for heat bath for 15-20 minutes; s3, differentiation culture: inoculating the callus subjected to the hot bath treatment into a differentiation culture medium, and inducing tissue differentiation; wherein the differential culture medium is prepared from 1.2 mg / L to 1.8 mg / L of 1 / 2 MS, 6-BA and 0.04 mg / L to 0.06 mg / L of TDZ; s4, inducing rooting: cutting cluster buds obtained through differentiation culture into single buds without callus tissues, and inoculating the single buds into a rooting culture medium; wherein the rooting culture medium is prepared from 1 / 2 MS, 1.0 mg / L to 1.4 mg / L of IBA, 0.2 mg / L to 0.4 mg / L of NAA and 0.04 mg / L to 0.07 mg / L of brassinolide. By using the method provided by the invention, the tomato spotted wilf virus detoxification success rate in the tsaoko amomum fruit tissue culture process can be effectively improved.

The invention relates to a biogas slurry composite biological agent for improving grape quality and an application method thereof, and belongs to the technical field of grape planting. Aiming at the problems that the direct application effect of the existing biogas slurry is unstable, the nutrients are unbalanced, and the traditional chemical fertilizer is easy to cause soil hardening and fruit quality reduction, the invention provides a technical scheme of taking the biogas slurry as a basic solution and scientifically compounding functional additives. Each liter of basic liquid of the preparation comprises 1.0-3.0 g of potassium humate, 0.5-2.0 ml of sugar alcohol calcium and 0.5-2.0 g of carboxymethyl chitosan, and at least one of 0.3-1.0 g of bacillus subtilis powder with the effective viable count being greater than or equal to 10 billion / g or 0.1-0.3 ml of a brassinolide aqueous solution with the concentration being 0.01% is selectively added. The preparation is mainly used for improving the fruit quality of grapes, and high-yield and high-quality cultivation of the grapes is realized through multiple effects of synergistically providing nutrients, promoting absorption, enhancing stress resistance and the like.