The invention discloses an SlFMO1
gene and application thereof in regulation and control of growth and development of tomato plants, and belongs to the technical field of
gene engineering. According to the method, a
CRISPR / Cas9
gene editing technology is utilized, firstly, a
CRISPR / Cas9-gRNA vector is constructed to accurately edit an SlFMO1 gene in tomatoes, GV3101
agrobacterium tumefaciens is utilized to conduct mediated transformation on the tomatoes, and
kanamycin serves as a resistance marker to conduct screening to obtain SlFMO1-deleted
mutant tomatoes.
Phenotype observation and analysis show that after the SlFMO1 gene is knocked out, the
plant height and the
plant width of a tomato
plant are obviously increased, the apical development is enhanced, the stem
diameter of the plant is increased, and the
inflorescence spacing is increased, which indicates that the SlFMO1 gene negatively regulates the growth and development of the tomato, and meanwhile, the expression of related genes synthesized by
auxin,
gibberellin and
brassinolide is obviously enhanced. The discovery has important theoretical significance and practical application value for follow-up cultivation of ideal tomato varieties.