The invention discloses a
tissue culture detoxification method of
Amomum tsao-ko, which comprises the following steps: S1, selecting an
Amomum tsao-ko
plant which grows robustly, taking a tender stem segment of the
Amomum tsao-ko
plant as an explant, disinfecting, inoculating into a
callus induction culture medium, culturing, and inducing the generation of
callus; wherein the
callus induction culture medium is prepared from 0.8 mg / L to 1.2 mg / L of MS + 2, 4-D, 0.4 mg / L to 0.6 mg / L of 6-BA and 3 mu mol / L to 5 mu mol / L of
chicoric acid; s2, heat treatment:
cutting the callus obtained in the step S1 into small pieces, and putting the small pieces into
sterile water at 40 + / -2 DEG C for
heat bath for 15-20 minutes; s3, differentiation culture: inoculating the callus subjected to the hot bath treatment into a differentiation culture medium, and inducing
tissue differentiation; wherein the differential culture medium is prepared from 1.2 mg / L to 1.8 mg / L of 1 / 2 MS, 6-BA and 0.04 mg / L to 0.06 mg / L of TDZ; s4, inducing rooting:
cutting cluster buds obtained through differentiation culture into single buds without callus tissues, and inoculating the single buds into a rooting culture medium; wherein the rooting culture medium is prepared from 1 / 2 MS, 1.0 mg / L to 1.4 mg / L of IBA, 0.2 mg / L to 0.4 mg / L of NAA and 0.04 mg / L to 0.07 mg / L of
brassinolide. By using the method provided by the invention, the tomato spotted wilf
virus detoxification success rate in the tsaoko
amomum fruit
tissue culture process can be effectively improved.