96 results about "Aminopurine" patented technology

The invention belongs to the technical field of plant tissue culture, and particularly relates to a tissue culture and rapid propagation culture medium and a tissue culture and rapid propagation method for culturing Shaniodendron subaequalis. The invention provides a tissue culture and rapid propagation culture medium and a tissue culture and rapid propagation method for culturing Shaniodendron subaequalis. Kinetin KT (Kinetin) in the tissue culture and rapid propagation culture medium provided by the invention can promote the generation of buds and the growth of adventitious buds. 6-BA is 6-benayl aminopurine and is used for promoting the growth of buds; tDZ is thiabendazole, and growth and development of buds are promoted. Under the combined action of KT, 6-BA and TDZ, the bud induction rate and the bud multiplication coefficient of the stem with the bud are improved. The invention further provides a tissue culture and rapid propagation method of the Shaniodendron subaequalis, stem segments subjected to induction culture are low in pollution rate, high in survival rate and low in browning rate; the lateral bud induction rate of the stem section is as high as 91%, and the multiplication coefficient, the rooting rate and the transplanting survival rate are high.

The invention provides a method for sterile sowing and efficient propagation of cymbidium faberi. The method comprises the following steps: pretreatment of cymbidium faberi seeds, sterile germination of the cymbidium faberi seeds, protocorm proliferation, differentiation, seedling strengthening and acclimatization and transplantation. The pretreatment comprises the steps of refrigerating hybrid seeds of cymbidium orchid at 4 DEG C, performing secondary disinfection with sodium hypochlorite, performing dark culture for 10-30 days after sowing, and then performing light culture, and the germination culture medium is prepared by taking 1 / 4 MS as a basic culture medium and adding 100-200ml / L of coconut water, 0.5-1.0 mg / L of 6-benayl aminopurine, 0.1-0.5 mg / L of naphthylacetic acid, 0.5-2.0 g / L of peptone and 0.5-1.0 g / L of activated carbon. According to the method, the seed germination rate can be effectively increased, the 60d seed germination rate reaches up to 85%, the pollution rate is reduced to 5% or below, 50.0 g / L inositol, 2.0 mg / L glycine and 2.0 mg / L nicotinic acid are added into a differentiation culture formula, the differentiation rate can be effectively increased, the leaf bud differentiation rate reaches up to 90%, meanwhile, rooting can be induced, the growth time of cymbidium faberi from seeds to seedlings is greatly shortened, a large number of high-quality seedlings can be obtained within a short time, and the method is suitable for large-scale popularization and application. Technical data are accumulated for cymbidium resource protection and new variety cultivation.

The invention belongs to the technical field of biology, and discloses a method and application for regulating and controlling the fermentation process of schizochytrium limacinum through plant hormones to improve the oil titer, and the method is characterized in that in the fermentation process of schizochytrium limacinum, one or more plant hormones are added into a culture medium to promote accumulation of oil. Plant hormones are added into a fermentation culture medium of schizochytrium limacinum, the plant hormones comprise gibberellin (GB), 6-benayl aminopurine (6-BAP), forchlorfenuron (CPPU) and brassinolide (BR), and the plant hormones promote grease accumulation by adjusting activity of enzymes related to fatty acid synthesis, so that the plant hormones can adjust metabolic pathways of the schizochytrium limacinum and promote grease accumulation. The synthesis efficiency and the yield of the grease are obviously improved.

The invention relates to the technical field of plant growth regulation, in particular to a pesticide preparation and method for promoting yield increase and branching of medicago sativa L. The pesticide preparation is prepared from, by weight, 1-4 parts of benayl aminopurine, 0.1 part of compound sodium nitrophenolate, 1-4 parts of brassinolide, 1-3 parts of triacontanol and 1-4 parts of uniconazole. When the medicago sativa grows to a seedling stage after being sown, the pesticide preparation is applied after the medicago sativa is cut on the ground, branching of the medicago sativa can be effectively promoted, the yield of the medicago sativa is increased, and the quality of the medicago sativa cannot be reduced.

The invention relates to the technical field of agricultural compositions, in particular to an agricultural composition for controlling vigorous growth of plants. The active ingredients of the agricultural composition for controlling vigorous growth of plants are composed of furfuryl aminopurine and prohexadione calcium, the prohexadione calcium and furfuryl aminopurine are mixed according to the mass ratio of (0.1-90): (0.01-50), the vigorous growth of plants can be improved and controlled, the vigorous growth control effect is better than the effect of each single agent through compounding of furfuryl aminopurine and prohexadione calcium, and the synergistic effect is obvious; in addition, a field test result shows that the yield is increased when the mixing mass ratio of furfurylaminopurine to prohexadione calcium is (0.1-90): (0.01-50), and the agricultural composition can be used for controlling vigorous growth and increasing yield of plants and has a good application prospect.

The invention relates to the field of cosmetics, in particular to a nicotiana benthamiana callus extract, a preparation method thereof and cosmetics. The method comprises the following steps: inoculating an induction medium with young leaves of nicotiana benthamiana as explants, and inducing the explants to form calluses; the induction culture medium is prepared from 1.0 to 3.0 mg / L of 6-benayl aminopurine; 0.2 to 1.0 mg / L of naphthylacetic acid; inoculating the callus on a subculture medium for multiplication culture; the subculture medium is prepared from the following components: 0.5 to 2.0 mg / L of 6-benayl aminopurine, 0.0 to 1.0 mg / L of naphthylacetic acid and 0.0 to 2.0 mg / L of 2, 4-dichlorphenoxyacetic acid; and extracting the calluses of the nicotiana benthamiana after multiplication culture by using water as a solvent according to a material-liquid ratio of 1: (5-20). According to the method, the Bensi tobacco callus with high dedifferentiation degree, good loosening degree and high growth speed is obtained, and the callus extract with the DPPH removal rate reaching 85% or above is obtained through extraction.

The invention belongs to the technical field of plant growth regulators, and particularly relates to a composition for flower and fruit thinning of apple trees, a suspending agent and application of the composition and the suspending agent. The flower and fruit thinning composition for apple trees provided by the invention comprises naphthylacetic acid and 6-benayl aminopurine, wherein the mass ratio of the naphthylacetic acid to the 6-benayl aminopurine is 1: (6.25-7). By compounding naphthylacetic acid and 6-benayl aminopurine in a specific proportion, the fruit and flower thinning effect is achieved, and in the early growth stage of apple trees, the flowering number can be reduced, the fruit number can be reduced, the fruit setting rate can be increased, sufficient nutrition can be guaranteed, the yield can be increased, and the fruit economic benefits can be increased.

The invention relates to the field of plant growth regulators, in particular to a conditioner group for improving the grain quality and the rice quality of direct seeding rice and a preparation and use method thereof.The preparation method of a conditioner in the initial heading stage comprises the following steps that 1, 6-benayl aminopurine is dissolved in a diluted hydrochloric acid solution in a assisted mode, and a 6-benayl aminopurine solution is obtained; step 2, adding choline chloride into the solution obtained in the step 1, stirring to obtain a mixed solution, transferring the mixed solution into a volumetric flask, and adjusting the pH value; 3, abscisic acid powder is dissolved with absolute ethyl alcohol, volume metering is carried out after dissolution to form a stock solution, and then PH adjustment is carried out. Two conditioners are included and sprayed in the initial heading stage and the full heading stage respectively, glumous flower degeneration can be reduced, the photosynthetic production capacity of the upper three leaves after flowering can be improved, assimilation substances are promoted to be transferred to grains, especially the filling state of vulnerable grains is improved, and the yield of the seedlings is increased. Finally, the grain number, the maturing rate, the grain weight and the yield are improved, meanwhile, the head rice rate of rice can be improved, and the protein content is reduced, so that the palatability of rice is improved.

The invention relates to the technical field of flower flowering phase regulation and control, and discloses a regulator and a method for promoting secondary flowering of crape myrtle, the regulator comprises 50 parts by weight of benzylaminopurine, 4.6-5 parts by weight of compound sodium nitrophenolate and more than or equal to 100 parts by weight of an amino acid composition; or 18 parts by weight of benzylaminopurine, 18 parts by weight of gibberellin, 4.6-5 parts by weight of compound sodium nitrophenolate and more than or equal to 100 parts by weight of an amino acid composition. The regulator provided by the invention comprehensively acts on multiple aspects of cell division, dormancy breaking, hormone regulation, nutrition supply, stress resistance enhancement and the like to jointly promote secondary blooming of the crape myrtle, increase the blooming amount and prolong the blooming period, and by virtue of multiple aspects of effects of pruning, fertilization, insect killing, plant growth regulator regulation and the like, the yield of the crape myrtle is increased. Secondary blooming of the crape myrtle is promoted, the blooming rate, the blooming duration and the blooming amount of secondary blooming are increased, and the growth vigor of the crape myrtle in the next year is not affected.

A pharmaceutical composition including a nucleoside analogue loaded into liposomes of a liposomal delivery system, wherein the nucleoside analogue is a purine analogue chosen from {[2-(6-amino-9H-purin-9-yl)quinazolin-4-yl]oxy}phosphonic acid or 2-(6-amino-9H-purin-9-yl) quinazolin-4-ol, or a pyrimidine analogue chosen from 4-amino-1-(4-hydroxyquinazolin-2-yl)-1,2-dihydropyrimidin-2-one or N-[1-(4-hydroxyquinazolin-2-yl)-2-oxo-1,2-dihydropyrimidin-4-yl]acetamide.

The invention relates to the technical field of chrysanthemum breeding methods, in particular to a method for accelerating chrysanthemum breeding by using a liquid culture medium, which comprises the following steps: cutting chrysanthemum stalks into square blocks of 1cm * 1cm in a culture box, flatly laying the stalks upwards on an induction culture solution soaking base, cutting stem tissues with axillary buds into small sections of 2-3cm, and obliquely inserting the small sections into the induction culture solution for culturing, mS + 6-BA 2.0 mg / L +, NAA 0.1 mg / L, alpha-naphthylacetic acid 2-4 mg / L, 6-benzylaminopurine and cane sugar are selected as culture solutions, in the propagation culture of the chrysanthemum, stem seedling sections grow fast, each section generates a plurality of clustered seedlings, the average multiplication coefficient is 4-7 times, the height of the stem seedlings is different, the clustered seedlings are 5-7 cm high when the seedlings are cultured in the nutrient solution for 2 months, and the adventitious bud multiplication rate of the chrysanthemum seedlings is increased. The method has the effect of accelerating chrysanthemum reproduction.

The present invention relates to a composition for plant, comprising 6-benzyl aminopurine (6-BAP), a fatty acid as a solvent, and an emulsifier, to an emulsifiable concentrate formulation (EC) for producing an oil-in-water emulsifiable concentrate or an oil-in-water emulsifiable concentrate using the same, to a method for preparing the compositions, to a plant growth or development regulating method, or to a fruit thinning method.

The present invention provides a salt of an aromatic amino purine derivative shown in Formula 2, and a preparation method and application thereof. The salt obtained by the present invention has good crystallinity and significantly improved solubility relative to the free form. The salt and the crystal form preferably have low hygroscopicity and can exist stably, so it is easier to make medicine than the free form of aromatic amino purine derivatives or other salts. (2).

The invention discloses a pesticide composition for increasing the yield in a soybean and corn strip-shaped composite planting mode, and belongs to the technical field of pesticides. The active ingredients of the pesticide composition comprise S-abscisic acid and furfuryl aminopurine, and the weight ratio of the S-abscisic acid to the furfuryl aminopurine is (1: 2)-(1: 10). When the S-abscisic acid and the furfurylaminopurine are mixed to be used in a soybean and corn strip-shaped composite planting mode, the photosynthesis of soybean and corn leaves can be remarkably improved, the influence of reduction of corn plant spacing, leaf overlapping shading and illumination weakening caused by high and large corn plants on soybean shading is reduced, the yield of the soybean and the corn is increased, and the yield of the soybean and the corn is increased. The effect of mixing the two components is obviously higher than that of a single component, the synergistic effect is obvious when the ratio of the two components is (1: 2)-(1: 10), and the optimal ratio is 1: 5. The dosage of the pesticide can be reduced, the use cost is reduced, and the safety to crops and the environment is improved.

The present application relates to the pharmaceutical use of 4-[9-(6-aminopurine)]-2(S)-hydroxybutyric acid methyl ester, in particular to its use in the preparation of a medicament for preventing and / or treating diseases associated with abnormal osteoclast activity. It has been found that 4-[9-(6-aminopurine)]-2(S)-hydroxybutyric acid methyl ester has a significant inhibitory effect on osteoclast differentiation, and intra-articular injection of 4-[9-(6-aminopurine)]-2(S)-hydroxybutyric acid methyl ester has a therapeutic effect on MIA-induced osteoarthritis. Therefore, 4-[9-(6-aminopurine)]-2(S)-hydroxybutyric acid methyl ester has the prospect of being developed into a therapeutic drug for diseases associated with abnormal osteoclast activity.

The invention provides a plant growth regulator for improving the maturing rate of leymus chinensis and a spraying method of the plant growth regulator. The plant growth regulator for improving the maturing rate of Chinese wildrye comprises one or more of 2, 4-dichlorphenoxyacetic acid, indole-3-acetic acid, gibberellin and 6-benayl aminopurine. The concentration of the 2, 4-dichlorphenoxyacetic acid is 10 mg / L, the concentration of the indole-3-acetic acid is 2.8 mg / L, the concentration of the gibberellin is 2.5 mg / L, and the concentration of the 6-benayl aminopurine is 5 mg / L. The plant growth regulator for improving the maturing rate of leymus chinensis and the spraying method thereof have the advantages of being high in preparation efficiency and relatively simple in spraying method.

The invention discloses an underground propagule composite excitant for ecological restoration and a preparation method thereof, and relates to the technical field of excitant preparation. The fertilizer is prepared from the following raw materials in parts by weight: 0.2 to 0.3 part of gibberellin, 0.2 to 0.5 part of 6-benayl aminopurine, 0.2 to 0.3 part of indolebutyric acid, 0.5 to 0.7 part of sodium nitroprusside, 0.4 to 0.5 part of hydrogen peroxide, 1 to 2 parts of jasmonic acid sustained-release microspheres, 5 to 7 parts of compound amino acid, 1 to 3 parts of compound microbial agent sustained-release microspheres, 2 to 3 parts of seaweed extract, 10 to 13 parts of humic acid, 2 to 3 parts of monopotassium phosphate, 60 to 74 parts of ethanol and 1000 parts of water. The underground propagule composite excitant provided by the invention is beneficial to promoting activation and germination of underground propagules, enhancing plant growth and stress resistance, improving soil micro-ecological functions, forming stable diversity communities and facilitating ecological restoration.

The invention discloses a preparation method of R-9-(2-phosphoric acid methoxy propyl) adenine, which comprises the following specific implementation processes: taking (R)-1-(6-amino-9H-purine-9-yl) propan-2-ol and (bis (benzyloxy) phosphoryl) methyl 4-methyl benzene sulfonate as initial raw materials, adding a solvent and alkali, replacing for three times by using nitrogen, stirring and reacting for 7-14 hours at 60-100 DEG C, after the reaction is finished, separating and purifying to obtain the R-9-(2-phosphoric acid methoxy propyl) adenine. Performing reduced pressure distillation to remove the solvent to obtain a brown yellow oily liquid, adding ethyl acetate, stirring, filtering to obtain a yellow filtrate, adding water into the filtrate for extraction and liquid separation, collecting an organic layer, adding anhydrous sodium sulfate, filtering, and performing rotary evaporation to remove ethyl acetate to obtain a yellow oily product; and finally, carrying out hydrogenation in an autoclave to obtain R-9-(2-phosphoric acid methoxy propyl) adenine. The invention designs a novel synthesis method, the hydrogenation yield of an autoclave is 94-98%, the total yield is 65-72%, and compared with the yield of 59.1% in the existing synthesis route, the yield is improved.

The invention belongs to the technical field of medicine synthesis, and relates to a synthetic method of a fluorescent 2-aminopurine derivative. In particular to a synthetic method of 2-amino-6-cyanopurine, which is characterized in that cyanamide (SM-1) and 5-amino-4-(cyanoformyl imidogen) imidazole (SM-2) are used as raw materials to synthesize the 2-amino-6-cyanopurine. The problems of complex operation, long reaction time, unstable yield and the like in the prior art can be effectively avoided.

This invention discloses a culture medium for Antrodia camphorata mycelium, which consists of two parts: a solid medium and a liquid medium. The liquid medium contains glucose, peptone, anhydrous magnesium sulfate, and plant hormones; the plant hormones include one or more of indolebutyric acid, lignin, gibberellin, 6-benzylaminopurine, and 24-epibrassinolide. The solid medium contains carrot powder, potato powder, glucose, and agar powder; the solid medium also contains plant hormones, including one or more of indolebutyric acid, lignin, gibberellin, 6-benzylaminopurine, and 24-epibrassinolide. During cultivation, activated mycelium is first inoculated onto the solid medium to form high-quality colonies, and then inoculated onto the liquid medium for further expansion. This method significantly increases mycelium yield and the production of crude polysaccharides and crude triterpenes, providing strong technical support for the development and utilization of Antrodia camphorata.

The invention discloses a culture method for rapid rooting of gingko aseptic seedlings, and relates to the technical field of gingko tissue culture. The culture method for rapid rooting of the gingko aseptic seedlings comprises the following steps: inoculating the gingko aseptic seedlings into a first rooting culture medium for culture, and then transferring the gingko aseptic seedlings into a second rooting culture medium for culture, the second rooting culture medium is an MS culture medium containing 0.1 to 0.2 mg / L of 6-benayl aminopurine, 0.5 to 1.5 mg / L of indolebutyric acid, 15 to 25 mL / L of gingko sterile main root juice, 7 to 9 mL / L of gingko sterile cotyledon juice, 25 to 35 g / L of sucrose and 6 to 8 g / L of agar. The invention effectively solves the problems of lack of staged regulation and control, single culture medium component, poor functionality, unreasonable hormone proportion and the like in the prior art.

This invention discloses a method for in vitro preservation of monk fruit, comprising the following steps: inoculating monk fruit seedlings into an in vitro culture medium for in vitro preservation culture, wherein the in vitro culture medium comprises 1 / 2 MS, 0.01-0.03 mg / L 6-benzylaminopurine, 0.6-1.4 mg / L quinacrine, 0.05-0.15 mg / L dextrin, 0.2-0.8 g / L activated carbon, 0.3-0.7 mg / L white tea extract, 0.01-0.03 mg / L amino acids, 0.01-0.03 mg / L brassinolide, and 0.5-1.5 mg / L eucalyptus leaf infusion. This invention, by adding white tea extract and eucalyptus leaf infusion to the in vitro culture medium, can effectively extend the in vitro preservation time of monk fruit.

The invention provides a plant regulator for inhibiting thin and acerbity of litchi pulp as well as a preparation method and application thereof, and the plant regulator is prepared from the following raw materials in parts by weight: 30-35 parts of 6-benayl aminopurine, 8-10 parts of 6-furfurylaminopurine, 5-8 parts of gibberellic acid A4, 3-5 parts of an active agent, 3-5 parts of a dispersing agent, 2-3 parts of amino-oligosaccharin, 1-3 parts of an emulsifying agent and 1-1.5 parts of 28 homobrassinolide. The active agent is one of sodium dodecyl benzene sulfonate, sodium butyl naphthalene sulfonate, dodecylphenol polyoxyethylene ether and sodium aminobenzene sulfonate. The dispersing agent is one of sulfated castor oil, sodium lignin sulfonate and oleoyl polyamino carboxylate. And the emulsifier is one of polyethylene glycol 600 monooleate and Tween 80. The plant regulating agent is sprayed in the flower withering period of litchi trees, and compared with the method without spraying the plant regulating agent, after the plant regulating agent is sprayed, the litchi pulp thickness, the fructose content, the total sugar ratio and the solid acid ratio are remarkably increased, and the quality of litchi fruits is effectively improved.

The invention relates to the technical field of saline-alkali soil improvement, in particular to a plant growth regulator for saline-alkali soil improvement and a preparation method thereof. The feed additive is prepared from the following components in parts by weight: 0.08 to 0.12 part of a modified regulator, 0.14 to 0.18 part of sucrose ester, 0.04 to 0.06 part of naphthylacetic acid, 0.12 to 0.18 part of gibberellic acid, 0.03 to 0.05 part of 6-benayl aminopurine, 0.8 to 1 part of vitamin C, 1 to 5 parts of mannitol, 1 to 3 parts of betaine and the balance of water, wherein the modification regulator is formed by surface modification of indoleacetic acid, sodium lignin sulfonate and millet colloid according to the mass ratio of 1: (5-10): (1.5-4.5); according to the plant growth regulator disclosed by the invention, the heteroauxin is wrapped and protected by the modified regulator through the millet colloid and the sodium lignin sulfonate, so that not only are the saline-alkali resistance and the plant growth promoting effect of the plant growth regulator improved, but also the plant growth regulator has a slow release effect, the action time of the plant growth regulator is prolonged, and stable growth stimulation is provided for plants; the use efficiency of the plant growth regulator is improved, and the use amount of the plant growth regulator and the influence on the environment are reduced.

The invention relates to a vase solution for preserving cut lily flowers of an OT hybrid line and application of the vase solution. The vase solution takes water as a solvent and comprises salicylic acid, citric acid, calcium chloride, 6-benayl aminopurine, aspirin, ascorbic acid, silver thiosulfate and cane sugar. The vase solution can improve the ornamental quality of cut flowers, remarkably prolong the service life of flowering branches and delay turbidity of the vase solution; in addition, the soluble sugar content and peroxidase content of the petals can be increased, and the malondialdehyde content of the petals can be reduced. The vase solution provided by the invention has the advantages of low cost, and simple and easy preparation and use.

The specification generally relates to compounds of Formula (I), or a stereoisomer or pharmaceutically acceptable salt thereof, wherein G, Ga, Gb, X, Y, R1, R2, Q1, Q2, and Q3 have any of the meanings defined herein, together with compositions containing them and their use in therapy. The compounds are inhibitors of the polymerase, DNA polymerase theta (Polθ or POLQ), and are thereby particularly useful in the treatment of cancer.

The invention relates to the technical field of culture medium manufacturing, in particular to a ford nervilia leaf tissue culture rhizome proliferation culture medium and a preparation method of the ford nervilia leaf tissue culture rhizome proliferation culture medium. The proliferation material is prepared from the following components in parts by weight: 2 to 4 parts of 6-benayl aminopurine, 0.3 to 0.7 part of naphthylacetic acid, 0.3 to 0.6 part of indolebutyric acid, 4 to 6 parts of gibberellin, 95 to 105 parts of novel coconut juice extract, 18 to 22 parts of novel pomegranate extract, 250 to 350 parts of cane sugar, 60 to 70 parts of agar and 3 to 5 parts of activated carbon. The improved pomegranate rind extract is subjected to ultrasonic and purification composite treatment, more antioxidant components such as ellagic acid and gallic acid are released, the antioxidant components, fermentation-enhanced amino acid and a nano silicon / selenium compound in the improved coconut juice extract form a dual antioxidant barrier, free radicals in plant cells are actively removed, oxidative damage is reduced, and the anti-aging effect is achieved. The hormone combination is precisely proportioned, so that lateral bud germination and stem elongation can be promoted.

The embodiment of the invention provides a golden camellia suspension cell culture method which comprises the following steps: golden camellia leaves are collected and inoculated into a WPM liquid culture medium containing hormone for culture, primary callus is formed through induction, and the hormone comprises naphthylacetic acid and 6-benayl aminopurine; and inoculating the primary callus into a WPM liquid culture medium containing auxin and cytokinin, and culturing to obtain the golden camellia suspension cells.

The invention belongs to the field of durian cultivation, and particularly relates to a durian flower forcing agent, a preparation method and application. According to the durian flower forcing agent, prohexadione calcium, potassium phosphite, benzyl benzyl aminopurine, thidiazuron and ethephon are reasonably combined, durian can be promoted to bloom in advance, the durian flower forcing agent is diluted and then sprayed to durian trees, budding of the durian trees can be achieved 9 days after spraying, and the budding time and the blooming time are shortened; meanwhile, the cluster number of durian flower buds can be remarkably increased, important guarantee is provided for increasing the yield of durian, and economic benefits of durian cultivation can be greatly increased.

The present invention belongs to the field of plant tissue culture technology, and specifically relates to a method and application for inducing callus tissue of aseptic monkey earring seedlings. The method comprises the following steps: taking the root system of the aseptic monkey earring seedlings as an explant and inoculating it in an induction medium for cultivation; the induction medium is an MS medium containing sucrose and agar, to which 0.4-0.6 mg / L of 6-benzylaminopurine, 0.1-0.5 mg / L of naphthaleneacetic acid, and 0-0.3 mg / L of gibberellins are added, and the pH is 5.8-5.9. The aseptic monkey earring seedlings cultivated by the present invention have lush branches and leaves, vigorous root growth, and have the advantages of convenient material collection and low contamination rate. The method can efficiently cultivate monkey earring root callus tissue, provide a reference for the asexual breeding of monkey earrings, and lay the foundation for inducing complete plants and realizing large-scale production of monkey earrings in the future.