23 results about "Aminopurine" patented technology

The invention relates to the field of plant growth regulators, in particular to a conditioner group for improving the grain quality and the rice quality of direct seeding rice and a preparation and use method thereof.The preparation method of a conditioner in the initial heading stage comprises the following steps that 1, 6-benayl aminopurine is dissolved in a diluted hydrochloric acid solution in a assisted mode, and a 6-benayl aminopurine solution is obtained; step 2, adding choline chloride into the solution obtained in the step 1, stirring to obtain a mixed solution, transferring the mixed solution into a volumetric flask, and adjusting the pH value; 3, abscisic acid powder is dissolved with absolute ethyl alcohol, volume metering is carried out after dissolution to form a stock solution, and then PH adjustment is carried out. Two conditioners are included and sprayed in the initial heading stage and the full heading stage respectively, glumous flower degeneration can be reduced, the photosynthetic production capacity of the upper three leaves after flowering can be improved, assimilation substances are promoted to be transferred to grains, especially the filling state of vulnerable grains is improved, and the yield of the seedlings is increased. Finally, the grain number, the maturing rate, the grain weight and the yield are improved, meanwhile, the head rice rate of rice can be improved, and the protein content is reduced, so that the palatability of rice is improved.

The present application relates to the pharmaceutical use of 4-[9-(6-aminopurine)]-2(S)-hydroxybutyric acid methyl ester, in particular to its use in the preparation of a medicament for preventing and / or treating diseases associated with abnormal osteoclast activity. It has been found that 4-[9-(6-aminopurine)]-2(S)-hydroxybutyric acid methyl ester has a significant inhibitory effect on osteoclast differentiation, and intra-articular injection of 4-[9-(6-aminopurine)]-2(S)-hydroxybutyric acid methyl ester has a therapeutic effect on MIA-induced osteoarthritis. Therefore, 4-[9-(6-aminopurine)]-2(S)-hydroxybutyric acid methyl ester has the prospect of being developed into a therapeutic drug for diseases associated with abnormal osteoclast activity.

The invention discloses an underground propagule composite excitant for ecological restoration and a preparation method thereof, and relates to the technical field of excitant preparation. The fertilizer is prepared from the following raw materials in parts by weight: 0.2 to 0.3 part of gibberellin, 0.2 to 0.5 part of 6-benayl aminopurine, 0.2 to 0.3 part of indolebutyric acid, 0.5 to 0.7 part of sodium nitroprusside, 0.4 to 0.5 part of hydrogen peroxide, 1 to 2 parts of jasmonic acid sustained-release microspheres, 5 to 7 parts of compound amino acid, 1 to 3 parts of compound microbial agent sustained-release microspheres, 2 to 3 parts of seaweed extract, 10 to 13 parts of humic acid, 2 to 3 parts of monopotassium phosphate, 60 to 74 parts of ethanol and 1000 parts of water. The underground propagule composite excitant provided by the invention is beneficial to promoting activation and germination of underground propagules, enhancing plant growth and stress resistance, improving soil micro-ecological functions, forming stable diversity communities and facilitating ecological restoration.

The invention belongs to the technical field of medicine synthesis, and relates to a synthetic method of a fluorescent 2-aminopurine derivative. In particular to a synthetic method of 2-amino-6-cyanopurine, which is characterized in that cyanamide (SM-1) and 5-amino-4-(cyanoformyl imidogen) imidazole (SM-2) are used as raw materials to synthesize the 2-amino-6-cyanopurine. The problems of complex operation, long reaction time, unstable yield and the like in the prior art can be effectively avoided.

This invention discloses a culture medium for Antrodia camphorata mycelium, which consists of two parts: a solid medium and a liquid medium. The liquid medium contains glucose, peptone, anhydrous magnesium sulfate, and plant hormones; the plant hormones include one or more of indolebutyric acid, lignin, gibberellin, 6-benzylaminopurine, and 24-epibrassinolide. The solid medium contains carrot powder, potato powder, glucose, and agar powder; the solid medium also contains plant hormones, including one or more of indolebutyric acid, lignin, gibberellin, 6-benzylaminopurine, and 24-epibrassinolide. During cultivation, activated mycelium is first inoculated onto the solid medium to form high-quality colonies, and then inoculated onto the liquid medium for further expansion. This method significantly increases mycelium yield and the production of crude polysaccharides and crude triterpenes, providing strong technical support for the development and utilization of Antrodia camphorata.

The invention discloses a culture method for rapid rooting of gingko aseptic seedlings, and relates to the technical field of gingko tissue culture. The culture method for rapid rooting of the gingko aseptic seedlings comprises the following steps: inoculating the gingko aseptic seedlings into a first rooting culture medium for culture, and then transferring the gingko aseptic seedlings into a second rooting culture medium for culture, the second rooting culture medium is an MS culture medium containing 0.1 to 0.2 mg / L of 6-benayl aminopurine, 0.5 to 1.5 mg / L of indolebutyric acid, 15 to 25 mL / L of gingko sterile main root juice, 7 to 9 mL / L of gingko sterile cotyledon juice, 25 to 35 g / L of sucrose and 6 to 8 g / L of agar. The invention effectively solves the problems of lack of staged regulation and control, single culture medium component, poor functionality, unreasonable hormone proportion and the like in the prior art.

The invention relates to a vase solution for preserving cut lily flowers of an OT hybrid line and application of the vase solution. The vase solution takes water as a solvent and comprises salicylic acid, citric acid, calcium chloride, 6-benayl aminopurine, aspirin, ascorbic acid, silver thiosulfate and cane sugar. The vase solution can improve the ornamental quality of cut flowers, remarkably prolong the service life of flowering branches and delay turbidity of the vase solution; in addition, the soluble sugar content and peroxidase content of the petals can be increased, and the malondialdehyde content of the petals can be reduced. The vase solution provided by the invention has the advantages of low cost, and simple and easy preparation and use.

The invention relates to the technical field of culture medium manufacturing, in particular to a ford nervilia leaf tissue culture rhizome proliferation culture medium and a preparation method of the ford nervilia leaf tissue culture rhizome proliferation culture medium. The proliferation material is prepared from the following components in parts by weight: 2 to 4 parts of 6-benayl aminopurine, 0.3 to 0.7 part of naphthylacetic acid, 0.3 to 0.6 part of indolebutyric acid, 4 to 6 parts of gibberellin, 95 to 105 parts of novel coconut juice extract, 18 to 22 parts of novel pomegranate extract, 250 to 350 parts of cane sugar, 60 to 70 parts of agar and 3 to 5 parts of activated carbon. The improved pomegranate rind extract is subjected to ultrasonic and purification composite treatment, more antioxidant components such as ellagic acid and gallic acid are released, the antioxidant components, fermentation-enhanced amino acid and a nano silicon / selenium compound in the improved coconut juice extract form a dual antioxidant barrier, free radicals in plant cells are actively removed, oxidative damage is reduced, and the anti-aging effect is achieved. The hormone combination is precisely proportioned, so that lateral bud germination and stem elongation can be promoted.

The embodiment of the invention provides a golden camellia suspension cell culture method which comprises the following steps: golden camellia leaves are collected and inoculated into a WPM liquid culture medium containing hormone for culture, primary callus is formed through induction, and the hormone comprises naphthylacetic acid and 6-benayl aminopurine; and inoculating the primary callus into a WPM liquid culture medium containing auxin and cytokinin, and culturing to obtain the golden camellia suspension cells.

The invention belongs to the field of durian cultivation, and particularly relates to a durian flower forcing agent, a preparation method and application. According to the durian flower forcing agent, prohexadione calcium, potassium phosphite, benzyl benzyl aminopurine, thidiazuron and ethephon are reasonably combined, durian can be promoted to bloom in advance, the durian flower forcing agent is diluted and then sprayed to durian trees, budding of the durian trees can be achieved 9 days after spraying, and the budding time and the blooming time are shortened; meanwhile, the cluster number of durian flower buds can be remarkably increased, important guarantee is provided for increasing the yield of durian, and economic benefits of durian cultivation can be greatly increased.

The invention relates to a group of metabolic markers and application thereof in preparation of products for screening and / or diagnosing depression. The metabolic marker is prepared from one or more of 4-oxo-2-propyl valeric acid, diethylstilbestrol, cyclo (leucine-valine) peptide, 6-benzylaminopurine and 3-carboxyl-4-methyl-5-pentyl-2-furanpropionic acid, and the metabolic marker is prepared from one or more of the following components: 4-oxo-2-propyl valeric acid, diethylstilbestrol, cyclo (leucine-valine) peptide, 6-benzylaminopurine and 3-carboxyl-4 According to the application, research finds that 4-oxo-2-propyl valeric acid, diethylstilbestrol, cyclo (leucine-valine) peptide, 6-benzylaminopurine and 3-carboxyl-4-methyl-5-pentyl-2-furanpropionic acid can be used as metabolic markers, and high-sensitivity and high-specificity screening diagnosis is carried out on the depression.

The present invention provides oligonucleotides useful for the treatment of Duchenne muscular dystrophy or Becker muscular dystrophy, compositions containing oligonucleotides, and methods for prevention, treatment, and / or delay. [Solution] An oligonucleotide is provided that comprises a 2'-O-methylRNA monomer and a phosphorothioate skeleton, and also comprises 5-methyluracil and / or 5-methylcytosine and / or a 2,6-diaminopurine base.

The application provides a kind of ophiopogon japonicus callus induction medium and its genetic transformation system construction method, belong to genetic transformation system construction technical field.A kind of ophiopogon japonicus callus induction medium is based on MS medium as base medium, further include 0.4~0.6 mg / L naphthalene acetic acid, 0.4~0.6 mg / L 2,4-D, 0.15~0.25 mg / L 6-benzylaminopurine, 28~32 g / L sucrose and 7~9 g / L agar, pH value is 5.8~6.0.The application utilizes the above-mentioned medium to carry out ophiopogon japonicus root callus induction culture, makes ophiopogon japonicus callus induction efficiency to 100%.Meanwhile, by optimizing the regeneration medium of callus, the regeneration rate reaches more than 90%.Based on two kinds of medium, the application establishes a kind of ophiopogon japonicus based on agrobacterium tumefaciens genetic transformation method, and the regeneration rate of transgenic seedling can reach more than 20%.

The invention discloses a giantleaf ardisia rhizome in-vitro preservation method and a culture medium, and belongs to the technical field of plant tissue culture. The method solves the technical problems that the giantleaf ardisia rhizome tissue culture seedlings grow too fast under the normal temperature condition and need to be transferred frequently, the existing low-temperature storage cost is high, and genetic variation is easily caused by using a growth inhibitor. A sterile stem segment with 1-2 axillary buds is inoculated to a specific preservation culture medium, the culture medium is based on improved MS, the content of macroelements in the culture medium is 0.5-1.0 time of the MS standard amount, and the culture medium contains 0.1-0.5 mg / L of 6-benzylaminopurine, 20-30 g / L of cane sugar and 0.5 g / L of activated carbon; during culture, dark culture is firstly performed at 25 + / -2 DEG C for 7-10 days, and then continuous preservation is performed under illumination of 40-60 [mu] mol.m <-2 >. S <-1 >. The method can realize long-term preservation at normal temperature, and is mainly used for in-vitro preservation of germplasm resources of giantleaf ardisia rhizome and continuous supply of seedlings.

This invention relates to a composition with plant growth regulating function, its preparation method, and its application. The composition comprises compound I and compound II; compound I is (2R,3R,4S)-2-(6-amino-9H-purin-9-yl)-2-(hydroxymethyl)-5-methylenetetrahydrofuran-3,4-diol; and compound II is (2R,3R,4S)-2-(6-amino-9H-purin-9-yl)-2,5-(dihydroxymethyl)-tetrahydrofuran-3,4-diol; wherein the mass ratio of compound I to compound II is 1:1 to 110:1. The specific proportions of compound I and compound II in the composition result in a better effect on regulating plant growth.

The present invention relates to an improved process for the preparation of (((1-(6-amino-9H-purin-9-yl)propan-2-yloxy)methyl)(phenoxy)phosphoryloxy)methyl pivalate Compound (1) or its pharmaceutically acceptable salts thereof. The compound is useful as nucleotide reverse transcriptase inhibitor in therapy for diseases which respond to inhibition of protein kinase activity in humans in need thereof.

The invention relates to the technical field of biology, in particular to a preparation and purification method of 2 '-fluoro-2'-deoxyguanosine. The invention discloses a preparation and purification method of 2 '-fluoro-2'-deoxyguanosine.The preparation method comprises the following steps that 2, 6-diaminopurine and 2 '-fluoro-2'-deoxyuridine generate 2 '-fluoro-2, 6-diaminopine-2'-deoxynucleoside under the catalysis of thymine nucleoside phosphorylase and purine nucleoside phosphorylase, filtering is conducted, and the 2 '-fluoro-2, 6-diaminopine-2'-deoxyguanosine is obtained. Then adenosine deaminase is added for catalysis, and 2 '-fluoro-2'-deoxyguanosine is generated; biosynthesis of the 2 '-fluoro-2'-deoxyguanosine is divided into two stages, the intermediate 2 '-fluoro-2, 6-diaminopine-2'-deoxynucleoside is synthesized firstly, and then deamination is performed to form the 2 '-fluoro-2'-deoxyguanosine, so that the problem that the conversion rate is extremely low when guanine with low solubility is taken as a substrate is solved.

The invention discloses a method for improving the flavone content and oxidation resistance of auricularia polytricha by using 6-benayl aminopurine, and belongs to the technical field of edible fungus cultivation. It is found and confirmed for the first time that 10 mg / L of 6-BA can significantly improve the flavone content and in-vitro oxidation resistance (DPPH and FRAP) of auricularia polytricha mycelia, and does not have an inhibition effect on mycelia growth. Through conjoint analysis of transcriptome and metabonomics, the molecular mechanism that 6-BA promotes accumulation of precursor substances by up-regulating expression of key genes such as PAL, 4CL, DFR and OMT in a flavonoid biosynthetic pathway so as to drive flavonoid synthesis is clarified from the molecular level. The invention provides an effective method for improving the nutritional value of the auricularia polytricha and developing functional products, and lays a theoretical foundation for the subsequent directional regulation and control of the flavone synthesis of the auricularia polytricha by means of genetic engineering, metabolic engineering and the like.

This invention discloses a highly efficient suspension culture method for Rhododendron simsii cells, belonging to the field of plant cell culture and natural product production technology. This method uses TDZ (thiazuron) instead of traditional 6-BA (6-benzylaminopurine) as a cytokinin, combined with four-stage hormone gradient regulation, LC-MS / MS (liquid chromatography-tandem mass spectrometry) dynamic monitoring of endogenous hormones, and staged suspension culture technology to establish a stable Rhododendron simsii suspension cell line. Compared with existing technologies, this method increases callus induction rate from 60% to 90%, reduces browning rate from 22% to 3%, increases cell proliferation from 2.85 times to 4.25 times, increases flavonoid content from 20.8 mg / g to 38.6 mg / g, increases purity from 72.3% to 93.5%, and reduces resource consumption by 97.8%, achieving efficient, green, and sustainable production of Rhododendron simsii flavonoids.

This invention relates to the field of plant tissue culture technology, and in particular to a culture medium for inhibiting browning of Ganoderma lucidum explants and improving callus induction rate, and its application. This invention provides an MS-based culture medium, further comprising 2,4-dichlorophenoxyacetic acid (2,4-D), naphthaleneacetic acid (NAA), 6-furfurylaminopurine (KT), polyvinylpyrrolidone (PVP), sucrose, and agar, which inhibits browning of Ganoderma lucidum explants and improves callus induction rate. The pH of this culture medium is 5.83-5.85. Examples show that the culture medium provided by this invention can significantly improve the callus induction rate of Ganoderma lucidum. Furthermore, the callus obtained from the callus induction medium can be subcultured to obtain a large amount of loosely textured and stable callus, providing a high-quality "seed source" for the industrial production of Ganoderma lucidum suspension cells.

The invention discloses a saussurea involucrata plant propagation method, and relates to the technical field of saussurea involucrata plant propagation. Performing vacuum pulse pressure induction; detoxifying and cleaning; and sine wave variable-pressure multiplication culture. According to the method, the 6-benayl aminopurine-lanthanum-cyclodextrin inclusion compound is matched with a vacuum pulse pressure induction process and a sine wave variable pressure enrichment culture process, efficient permeation is realized through a low-surface tension instantaneous permeation induction liquid, and cell sensitivity is recovered in combination with an intermittent reset protocol; the problems of low induction rate, serious browning, mechanical fatigue of cells and the like in the prior art are effectively solved, and the propagation coefficient and quality of saussurea involucrata are improved.

The invention belongs to the technical field of plant culture, and particularly relates to a culture medium and a culture method for one-step seedling tissue culture of deutzia grandiflora. The culture medium comprises the following components: a basic culture medium, 6-benayl aminopurine, 6-furfuryl aminopurine and auxin. According to the culture medium and the culture method for one-step induction of the regenerated plant from the deutzia grandiflora explant, vegetative propagation can be performed on deutzia grandiflora simply, quickly, efficiently and at low cost, so that a technical support is provided for large-scale seedling culture.

This invention discloses a culture medium for dwarfing *Cibotium barometz*, belonging to the field of plant tissue culture technology. The culture medium comprises the following components: 1 / 6-1 / 3 MS, sucrose 2-6 g / L, 6-benzylaminopurine 0.03-0.15 mg / L, agar 4-8 g / L, indolebutyric acid 0.01-0.08 mg / L, paclobutrazol 0.25-0.8 mg / L, and activated carbon 0.3-1.0 g / L. This culture medium can synergistically inhibit excessive petiole growth and delay leaf senescence, thus solving the technical problem that *Cibotium barometz* cannot maintain its miniature and graceful morphology and require no maintenance in a closed microenvironment for a long period.