40 results about "Aminopurine" patented technology

The invention relates to the field of plant growth regulators, in particular to a conditioner group for improving the grain quality and the rice quality of direct seeding rice and a preparation and use method thereof.The preparation method of a conditioner in the initial heading stage comprises the following steps that 1, 6-benayl aminopurine is dissolved in a diluted hydrochloric acid solution in a assisted mode, and a 6-benayl aminopurine solution is obtained; step 2, adding choline chloride into the solution obtained in the step 1, stirring to obtain a mixed solution, transferring the mixed solution into a volumetric flask, and adjusting the pH value; 3, abscisic acid powder is dissolved with absolute ethyl alcohol, volume metering is carried out after dissolution to form a stock solution, and then PH adjustment is carried out. Two conditioners are included and sprayed in the initial heading stage and the full heading stage respectively, glumous flower degeneration can be reduced, the photosynthetic production capacity of the upper three leaves after flowering can be improved, assimilation substances are promoted to be transferred to grains, especially the filling state of vulnerable grains is improved, and the yield of the seedlings is increased. Finally, the grain number, the maturing rate, the grain weight and the yield are improved, meanwhile, the head rice rate of rice can be improved, and the protein content is reduced, so that the palatability of rice is improved.

The invention relates to the technical field of flower flowering phase regulation and control, and discloses a regulator and a method for promoting secondary flowering of crape myrtle, the regulator comprises 50 parts by weight of benzylaminopurine, 4.6-5 parts by weight of compound sodium nitrophenolate and more than or equal to 100 parts by weight of an amino acid composition; or 18 parts by weight of benzylaminopurine, 18 parts by weight of gibberellin, 4.6-5 parts by weight of compound sodium nitrophenolate and more than or equal to 100 parts by weight of an amino acid composition. The regulator provided by the invention comprehensively acts on multiple aspects of cell division, dormancy breaking, hormone regulation, nutrition supply, stress resistance enhancement and the like to jointly promote secondary blooming of the crape myrtle, increase the blooming amount and prolong the blooming period, and by virtue of multiple aspects of effects of pruning, fertilization, insect killing, plant growth regulator regulation and the like, the yield of the crape myrtle is increased. Secondary blooming of the crape myrtle is promoted, the blooming rate, the blooming duration and the blooming amount of secondary blooming are increased, and the growth vigor of the crape myrtle in the next year is not affected.

The present application relates to the pharmaceutical use of 4-[9-(6-aminopurine)]-2(S)-hydroxybutyric acid methyl ester, in particular to its use in the preparation of a medicament for preventing and / or treating diseases associated with abnormal osteoclast activity. It has been found that 4-[9-(6-aminopurine)]-2(S)-hydroxybutyric acid methyl ester has a significant inhibitory effect on osteoclast differentiation, and intra-articular injection of 4-[9-(6-aminopurine)]-2(S)-hydroxybutyric acid methyl ester has a therapeutic effect on MIA-induced osteoarthritis. Therefore, 4-[9-(6-aminopurine)]-2(S)-hydroxybutyric acid methyl ester has the prospect of being developed into a therapeutic drug for diseases associated with abnormal osteoclast activity.

The invention discloses an underground propagule composite excitant for ecological restoration and a preparation method thereof, and relates to the technical field of excitant preparation. The fertilizer is prepared from the following raw materials in parts by weight: 0.2 to 0.3 part of gibberellin, 0.2 to 0.5 part of 6-benayl aminopurine, 0.2 to 0.3 part of indolebutyric acid, 0.5 to 0.7 part of sodium nitroprusside, 0.4 to 0.5 part of hydrogen peroxide, 1 to 2 parts of jasmonic acid sustained-release microspheres, 5 to 7 parts of compound amino acid, 1 to 3 parts of compound microbial agent sustained-release microspheres, 2 to 3 parts of seaweed extract, 10 to 13 parts of humic acid, 2 to 3 parts of monopotassium phosphate, 60 to 74 parts of ethanol and 1000 parts of water. The underground propagule composite excitant provided by the invention is beneficial to promoting activation and germination of underground propagules, enhancing plant growth and stress resistance, improving soil micro-ecological functions, forming stable diversity communities and facilitating ecological restoration.

The invention belongs to the technical field of medicine synthesis, and relates to a synthetic method of a fluorescent 2-aminopurine derivative. In particular to a synthetic method of 2-amino-6-cyanopurine, which is characterized in that cyanamide (SM-1) and 5-amino-4-(cyanoformyl imidogen) imidazole (SM-2) are used as raw materials to synthesize the 2-amino-6-cyanopurine. The problems of complex operation, long reaction time, unstable yield and the like in the prior art can be effectively avoided.

This invention discloses a culture medium for Antrodia camphorata mycelium, which consists of two parts: a solid medium and a liquid medium. The liquid medium contains glucose, peptone, anhydrous magnesium sulfate, and plant hormones; the plant hormones include one or more of indolebutyric acid, lignin, gibberellin, 6-benzylaminopurine, and 24-epibrassinolide. The solid medium contains carrot powder, potato powder, glucose, and agar powder; the solid medium also contains plant hormones, including one or more of indolebutyric acid, lignin, gibberellin, 6-benzylaminopurine, and 24-epibrassinolide. During cultivation, activated mycelium is first inoculated onto the solid medium to form high-quality colonies, and then inoculated onto the liquid medium for further expansion. This method significantly increases mycelium yield and the production of crude polysaccharides and crude triterpenes, providing strong technical support for the development and utilization of Antrodia camphorata.

The invention discloses a culture method for rapid rooting of gingko aseptic seedlings, and relates to the technical field of gingko tissue culture. The culture method for rapid rooting of the gingko aseptic seedlings comprises the following steps: inoculating the gingko aseptic seedlings into a first rooting culture medium for culture, and then transferring the gingko aseptic seedlings into a second rooting culture medium for culture, the second rooting culture medium is an MS culture medium containing 0.1 to 0.2 mg / L of 6-benayl aminopurine, 0.5 to 1.5 mg / L of indolebutyric acid, 15 to 25 mL / L of gingko sterile main root juice, 7 to 9 mL / L of gingko sterile cotyledon juice, 25 to 35 g / L of sucrose and 6 to 8 g / L of agar. The invention effectively solves the problems of lack of staged regulation and control, single culture medium component, poor functionality, unreasonable hormone proportion and the like in the prior art.

This invention discloses a method for in vitro preservation of monk fruit, comprising the following steps: inoculating monk fruit seedlings into an in vitro culture medium for in vitro preservation culture, wherein the in vitro culture medium comprises 1 / 2 MS, 0.01-0.03 mg / L 6-benzylaminopurine, 0.6-1.4 mg / L quinacrine, 0.05-0.15 mg / L dextrin, 0.2-0.8 g / L activated carbon, 0.3-0.7 mg / L white tea extract, 0.01-0.03 mg / L amino acids, 0.01-0.03 mg / L brassinolide, and 0.5-1.5 mg / L eucalyptus leaf infusion. This invention, by adding white tea extract and eucalyptus leaf infusion to the in vitro culture medium, can effectively extend the in vitro preservation time of monk fruit.

The invention relates to a vase solution for preserving cut lily flowers of an OT hybrid line and application of the vase solution. The vase solution takes water as a solvent and comprises salicylic acid, citric acid, calcium chloride, 6-benayl aminopurine, aspirin, ascorbic acid, silver thiosulfate and cane sugar. The vase solution can improve the ornamental quality of cut flowers, remarkably prolong the service life of flowering branches and delay turbidity of the vase solution; in addition, the soluble sugar content and peroxidase content of the petals can be increased, and the malondialdehyde content of the petals can be reduced. The vase solution provided by the invention has the advantages of low cost, and simple and easy preparation and use.

The specification generally relates to compounds of Formula (I), or a stereoisomer or pharmaceutically acceptable salt thereof, wherein G, Ga, Gb, X, Y, R1, R2, Q1, Q2, and Q3 have any of the meanings defined herein, together with compositions containing them and their use in therapy. The compounds are inhibitors of the polymerase, DNA polymerase theta (Polθ or POLQ), and are thereby particularly useful in the treatment of cancer.

The invention relates to the technical field of culture medium manufacturing, in particular to a ford nervilia leaf tissue culture rhizome proliferation culture medium and a preparation method of the ford nervilia leaf tissue culture rhizome proliferation culture medium. The proliferation material is prepared from the following components in parts by weight: 2 to 4 parts of 6-benayl aminopurine, 0.3 to 0.7 part of naphthylacetic acid, 0.3 to 0.6 part of indolebutyric acid, 4 to 6 parts of gibberellin, 95 to 105 parts of novel coconut juice extract, 18 to 22 parts of novel pomegranate extract, 250 to 350 parts of cane sugar, 60 to 70 parts of agar and 3 to 5 parts of activated carbon. The improved pomegranate rind extract is subjected to ultrasonic and purification composite treatment, more antioxidant components such as ellagic acid and gallic acid are released, the antioxidant components, fermentation-enhanced amino acid and a nano silicon / selenium compound in the improved coconut juice extract form a dual antioxidant barrier, free radicals in plant cells are actively removed, oxidative damage is reduced, and the anti-aging effect is achieved. The hormone combination is precisely proportioned, so that lateral bud germination and stem elongation can be promoted.

The embodiment of the invention provides a golden camellia suspension cell culture method which comprises the following steps: golden camellia leaves are collected and inoculated into a WPM liquid culture medium containing hormone for culture, primary callus is formed through induction, and the hormone comprises naphthylacetic acid and 6-benayl aminopurine; and inoculating the primary callus into a WPM liquid culture medium containing auxin and cytokinin, and culturing to obtain the golden camellia suspension cells.

The invention belongs to the field of durian cultivation, and particularly relates to a durian flower forcing agent, a preparation method and application. According to the durian flower forcing agent, prohexadione calcium, potassium phosphite, benzyl benzyl aminopurine, thidiazuron and ethephon are reasonably combined, durian can be promoted to bloom in advance, the durian flower forcing agent is diluted and then sprayed to durian trees, budding of the durian trees can be achieved 9 days after spraying, and the budding time and the blooming time are shortened; meanwhile, the cluster number of durian flower buds can be remarkably increased, important guarantee is provided for increasing the yield of durian, and economic benefits of durian cultivation can be greatly increased.

The invention belongs to the technical field of solar cell manufacturing, and provides a perovskite solar cell and a preparation method thereof. The perovskite solar cell comprises a conductive substrate, an electron transport layer, a perovskite layer, a passivation modification layer, a hole transport layer and an electrode layer. The perovskite layer is a perovskite layer modified by 2-aminopurine; and a passivation material in the passivation modification layer is 2-mercaptoethane-1-sulfonamide hydriodate. According to the invention, 2-aminopurine and 2-mercaptoethane-1-sulfonamide hydriodate are used for synergistically passivating the perovskite layer, so that the crystal defect and vacancy defect of the perovskite thin film are reduced, and the crystal quality of the perovskite thin film is improved, thereby improving the open-circuit voltage, short-circuit current density and energy conversion efficiency of the perovskite solar cell; and commercial application development of the perovskite solar cell is promoted.

The invention relates to a group of metabolic markers and application thereof in preparation of products for screening and / or diagnosing depression. The metabolic marker is prepared from one or more of 4-oxo-2-propyl valeric acid, diethylstilbestrol, cyclo (leucine-valine) peptide, 6-benzylaminopurine and 3-carboxyl-4-methyl-5-pentyl-2-furanpropionic acid, and the metabolic marker is prepared from one or more of the following components: 4-oxo-2-propyl valeric acid, diethylstilbestrol, cyclo (leucine-valine) peptide, 6-benzylaminopurine and 3-carboxyl-4 According to the application, research finds that 4-oxo-2-propyl valeric acid, diethylstilbestrol, cyclo (leucine-valine) peptide, 6-benzylaminopurine and 3-carboxyl-4-methyl-5-pentyl-2-furanpropionic acid can be used as metabolic markers, and high-sensitivity and high-specificity screening diagnosis is carried out on the depression.

The invention discloses a formula of a broad-spectrum culture medium adapted to sterile sowing of multiple species of Chinese orchid, and relates to the technical field of plant tissue culture, the broad-spectrum culture medium adopts a 1 / 2MS basic culture medium, the dosage of a macroelement mother solution is halved, and the dosage of ferric salt, microelement and vitamin mother solution is kept unchanged; 10 mg of modified graphene, 1.5 mg to 2.5 mg of 6-benayl aminopurine (6-BA), 0.3 mg to 0.8 mg of naphthylacetic acid (NAA), 0.2 mg to 0.8 mg of zeatin (ZT), 0.05 mg to 0.2 mg of indolebutyric acid (IBA), 1.2 g to 2.2 g of lactoalbumin hydrolysate, 0.6 g to 1.0 g of activated carbon, 22 g to 32 g of saccharose and 5.5 g to 7.5 g of agar are added into every 1 L of the basic culture medium. The pH value of the culture medium is adjusted to 5.2-5.8. By adding the modified graphene into the culture medium, germination of Chinese orchid seeds is effectively promoted. Meanwhile, a complete nutrition system and a root system induction mechanism enhance the growth vigor of the seedlings and improve the survival rate of the seedlings, and meanwhile the universality of the culture medium is remarkably improved through the formula of the culture medium.

The present invention provides oligonucleotides useful for the treatment of Duchenne muscular dystrophy or Becker muscular dystrophy, compositions containing oligonucleotides, and methods for prevention, treatment, and / or delay. [Solution] An oligonucleotide is provided that comprises a 2'-O-methylRNA monomer and a phosphorothioate skeleton, and also comprises 5-methyluracil and / or 5-methylcytosine and / or a 2,6-diaminopurine base.

The application provides a crystal form of a compound (2R, 3R, 4S)-2-(6-amino-9H-purin-9-yl)-2-(hydroxymethyl)-5-methylenetetrahydrofuran-3, 4-diol, which is selected from the crystal form F; wherein the crystal form F has characteristic diffraction peaks of an X-ray powder diffraction pattern expressed by 2θ values ± 0.2° using Cu-Kα radiation, and the characteristic diffraction peaks include 7.43, 13.07 and 13.69. The crystal form has good stability, can be kept stable under light, high temperature, high humidity and acceleration conditions, and can better meet the requirements of pesticides in production, processing, transportation and storage.

The application provides a kind of ophiopogon japonicus callus induction medium and its genetic transformation system construction method, belong to genetic transformation system construction technical field.A kind of ophiopogon japonicus callus induction medium is based on MS medium as base medium, further include 0.4~0.6 mg / L naphthalene acetic acid, 0.4~0.6 mg / L 2,4-D, 0.15~0.25 mg / L 6-benzylaminopurine, 28~32 g / L sucrose and 7~9 g / L agar, pH value is 5.8~6.0.The application utilizes the above-mentioned medium to carry out ophiopogon japonicus root callus induction culture, makes ophiopogon japonicus callus induction efficiency to 100%.Meanwhile, by optimizing the regeneration medium of callus, the regeneration rate reaches more than 90%.Based on two kinds of medium, the application establishes a kind of ophiopogon japonicus based on agrobacterium tumefaciens genetic transformation method, and the regeneration rate of transgenic seedling can reach more than 20%.

The application provides a crystal form of a compound (2R, 3R, 4S)-2-(6-amino-9H-purin-9-yl)-2-(hydroxymethyl)-5-methylenetetrahydrofuran-3, 4-diol, which is selected from crystal form D and crystal form E; wherein the characteristic diffraction peak of the X-ray powder diffraction pattern of the crystal form D using Cu-K alpha radiation, expressed by a 2 theta value ± 0.2°, includes 5.16, 7.26, 14.53 and 17.52; the characteristic diffraction peak of the X-ray powder diffraction pattern of the crystal form E using Cu-K alpha radiation, expressed by a 2 theta value ± 0.2°, includes 5.06, 7.18, 13.59 and 14.45. The crystal form has good stability and can maintain stability under light, high temperature, high humidity and acceleration conditions, and can better meet the requirements of pesticides in production, processing, transportation and storage.

The invention discloses a giantleaf ardisia rhizome in-vitro preservation method and a culture medium, and belongs to the technical field of plant tissue culture. The method solves the technical problems that the giantleaf ardisia rhizome tissue culture seedlings grow too fast under the normal temperature condition and need to be transferred frequently, the existing low-temperature storage cost is high, and genetic variation is easily caused by using a growth inhibitor. A sterile stem segment with 1-2 axillary buds is inoculated to a specific preservation culture medium, the culture medium is based on improved MS, the content of macroelements in the culture medium is 0.5-1.0 time of the MS standard amount, and the culture medium contains 0.1-0.5 mg / L of 6-benzylaminopurine, 20-30 g / L of cane sugar and 0.5 g / L of activated carbon; during culture, dark culture is firstly performed at 25 + / -2 DEG C for 7-10 days, and then continuous preservation is performed under illumination of 40-60 [mu] mol.m <-2 >. S <-1 >. The method can realize long-term preservation at normal temperature, and is mainly used for in-vitro preservation of germplasm resources of giantleaf ardisia rhizome and continuous supply of seedlings.

The invention relates to alkylguanine transferase substrate molecules. In general formula (I) SB is a moiety that can be enzymatically cleaved from the rest of the molecule by an enzyme having an alkyltransferase activity selected from the group consisting of a 2-aminopyrimidinyl moiety, an aminopyridinyl moiety, and a 2-aminopurinyl moiety. Also described are molecules of general formula (II) and general formula (III) L is an optional linker, RN is H, methyl or ethyl or their deuterated or fluorinated derivatives, and R is selected from a detectable label and a selectively reactive moiety.

This invention relates to a composition with plant growth regulating function, its preparation method, and its application. The composition comprises compound I and compound II; compound I is (2R,3R,4S)-2-(6-amino-9H-purin-9-yl)-2-(hydroxymethyl)-5-methylenetetrahydrofuran-3,4-diol; and compound II is (2R,3R,4S)-2-(6-amino-9H-purin-9-yl)-2,5-(dihydroxymethyl)-tetrahydrofuran-3,4-diol; wherein the mass ratio of compound I to compound II is 1:1 to 110:1. The specific proportions of compound I and compound II in the composition result in a better effect on regulating plant growth.

The present invention relates to an improved process for the preparation of (((1-(6-amino-9H-purin-9-yl)propan-2-yloxy)methyl)(phenoxy)phosphoryloxy)methyl pivalate Compound (1) or its pharmaceutically acceptable salts thereof. The compound is useful as nucleotide reverse transcriptase inhibitor in therapy for diseases which respond to inhibition of protein kinase activity in humans in need thereof.

The invention relates to the technical field of biology, in particular to a preparation and purification method of 2 '-fluoro-2'-deoxyguanosine. The invention discloses a preparation and purification method of 2 '-fluoro-2'-deoxyguanosine.The preparation method comprises the following steps that 2, 6-diaminopurine and 2 '-fluoro-2'-deoxyuridine generate 2 '-fluoro-2, 6-diaminopine-2'-deoxynucleoside under the catalysis of thymine nucleoside phosphorylase and purine nucleoside phosphorylase, filtering is conducted, and the 2 '-fluoro-2, 6-diaminopine-2'-deoxyguanosine is obtained. Then adenosine deaminase is added for catalysis, and 2 '-fluoro-2'-deoxyguanosine is generated; biosynthesis of the 2 '-fluoro-2'-deoxyguanosine is divided into two stages, the intermediate 2 '-fluoro-2, 6-diaminopine-2'-deoxynucleoside is synthesized firstly, and then deamination is performed to form the 2 '-fluoro-2'-deoxyguanosine, so that the problem that the conversion rate is extremely low when guanine with low solubility is taken as a substrate is solved.

The invention discloses a method for improving the flavone content and oxidation resistance of auricularia polytricha by using 6-benayl aminopurine, and belongs to the technical field of edible fungus cultivation. It is found and confirmed for the first time that 10 mg / L of 6-BA can significantly improve the flavone content and in-vitro oxidation resistance (DPPH and FRAP) of auricularia polytricha mycelia, and does not have an inhibition effect on mycelia growth. Through conjoint analysis of transcriptome and metabonomics, the molecular mechanism that 6-BA promotes accumulation of precursor substances by up-regulating expression of key genes such as PAL, 4CL, DFR and OMT in a flavonoid biosynthetic pathway so as to drive flavonoid synthesis is clarified from the molecular level. The invention provides an effective method for improving the nutritional value of the auricularia polytricha and developing functional products, and lays a theoretical foundation for the subsequent directional regulation and control of the flavone synthesis of the auricularia polytricha by means of genetic engineering, metabolic engineering and the like.

The invention provides a radix ophiopogonis callus induction culture medium and a construction method of a genetic transformation system of the radix ophiopogonis callus induction culture medium, and belongs to the technical field of genetic transformation system construction. The invention relates to a radix ophiopogonis callus induction culture medium, which takes an MS culture medium as a basic culture medium, and further comprises 0.4 to 0.6 mg / L of naphthylacetic acid, 0.4 to 0.6 mg / L of 2, 4-D, 0.15 to 0.25 mg / L of 6-benayl aminopurine, 28 to 32 g / L of sucrose and 7 to 9 g / L of agar, and the pH value is 5.8 to 6.0. The culture medium is used for carrying out callus induction culture on the radix ophiopogonis roots, so that the callus induction efficiency of the radix ophiopogonis reaches 100%. Meanwhile, by optimizing a regeneration culture medium of the calluses, the regeneration rate reaches 90% or above. Based on the two culture media, the invention establishes a genetic transformation method of radix ophiopogonis based on agrobacterium tumefaciens, so that the regeneration rate of transgenic seedlings can reach 20% or above.

The invention relates to the technical field of nutrient regulation of hydroponic plants, in particular to a nutrient regulation method suitable for growth of hydroponic alfalfa in different periods, which comprises the following steps: dividing the growth period of hydroponic alfalfa into a seedling period, a vegetative growth period, a squaring period and a flowering period, and dynamically adjusting nutrient solution components and environmental parameters for each period. The method specifically comprises the steps of promoting root development through low-concentration nitrogen and naphthylacetic acid in the seedling stage, optimizing the nitrogen-phosphorus ratio and conductivity in the vegetative growth stage, increasing potassium and boric acid in the squaring stage to prevent flower buds from falling off, reducing nitrogen in the flowering stage and supplementing 6-benayl aminopurine to delay senescence, and cooperatively regulating and controlling illumination and pH value in the squaring stage. The method can significantly improve the yield and crude protein content of hydroponic alfalfa, shorten the growth cycle, reduce the production cost, and meet the efficient and accurate requirements of modern agriculture.

The invention relates to a method for cultivating Xinjiang lithospermum erythrorhizon test-tube plantlets and a method for hardening the test-tube plantlets, and belongs to the technical field of plant tissue culture. The invention provides a proliferation culture medium suitable for tissue culture of Sinkiang lithospermum, the proliferation culture medium is based on an SH culture medium and comprises 6-benayl aminopurine, indoleacetic acid, coconut water, sucrose and agar, and the pH value of the proliferation culture medium is 5.6-6.0. When the method for culturing the Sinkiang lithospermum test-tube plantlets is adopted for tissue culture, the germination rate of Sinkiang lithospermum seeds can reach 96.7%, the multiplication coefficient can reach 13.5, the rooting rate can reach 96.3%, and when the Sinkiang lithospermum test-tube plantlets are subjected to seedling hardening by adopting the method for hardening the Sinkiang lithospermum test-tube plantlets, the obtained to-be-transplanted plantlets are good in root system development and good in transplanting effect. And the seedling hardening survival rate of the test-tube seedlings can reach more than 91.3%. The method provides technical support for rapid propagation and large-scale production of the Sinkiang lithospermum erythrorhizon, and resource protection and sustainable utilization of the Sinkiang lithospermum erythrorhizon are realized.

The invention relates to the technical field of flower flowering phase regulation and control, and discloses a regulator and a method for improving high-temperature flowering quality of petunia hybrida, the regulator comprises 0.1 part by weight of a component A, 33 parts by weight of a component B and more than or equal to 100 parts by weight of a component C; the component A is selected from at least one of brassinolide and an isomer thereof, the component B is benzylaminopurine, and the component C is an amino acid composition. According to the invention, brassinolide, benzylaminopurine and amino acid are compounded for use, and through mutual cooperation of different action ways and mechanisms, the flowering attenuation rate of petunia hybrida in a high-temperature environment is reduced, the flowering amount is obviously increased, and the yield of petunia hybrida is increased. Meanwhile, the blooming ability is also improved in a continuous high-temperature environment.