62 results about "Aminopurine" patented technology

The invention relates to the field of cosmetics, in particular to a nicotiana benthamiana callus extract, a preparation method thereof and cosmetics. The method comprises the following steps: inoculating an induction medium with young leaves of nicotiana benthamiana as explants, and inducing the explants to form calluses; the induction culture medium is prepared from 1.0 to 3.0 mg / L of 6-benayl aminopurine; 0.2 to 1.0 mg / L of naphthylacetic acid; inoculating the callus on a subculture medium for multiplication culture; the subculture medium is prepared from the following components: 0.5 to 2.0 mg / L of 6-benayl aminopurine, 0.0 to 1.0 mg / L of naphthylacetic acid and 0.0 to 2.0 mg / L of 2, 4-dichlorphenoxyacetic acid; and extracting the calluses of the nicotiana benthamiana after multiplication culture by using water as a solvent according to a material-liquid ratio of 1: (5-20). According to the method, the Bensi tobacco callus with high dedifferentiation degree, good loosening degree and high growth speed is obtained, and the callus extract with the DPPH removal rate reaching 85% or above is obtained through extraction.

The invention belongs to the technical field of plant growth regulators, and particularly relates to a composition for flower and fruit thinning of apple trees, a suspending agent and application of the composition and the suspending agent. The flower and fruit thinning composition for apple trees provided by the invention comprises naphthylacetic acid and 6-benayl aminopurine, wherein the mass ratio of the naphthylacetic acid to the 6-benayl aminopurine is 1: (6.25-7). By compounding naphthylacetic acid and 6-benayl aminopurine in a specific proportion, the fruit and flower thinning effect is achieved, and in the early growth stage of apple trees, the flowering number can be reduced, the fruit number can be reduced, the fruit setting rate can be increased, sufficient nutrition can be guaranteed, the yield can be increased, and the fruit economic benefits can be increased.

The invention relates to the field of plant growth regulators, in particular to a conditioner group for improving the grain quality and the rice quality of direct seeding rice and a preparation and use method thereof.The preparation method of a conditioner in the initial heading stage comprises the following steps that 1, 6-benayl aminopurine is dissolved in a diluted hydrochloric acid solution in a assisted mode, and a 6-benayl aminopurine solution is obtained; step 2, adding choline chloride into the solution obtained in the step 1, stirring to obtain a mixed solution, transferring the mixed solution into a volumetric flask, and adjusting the pH value; 3, abscisic acid powder is dissolved with absolute ethyl alcohol, volume metering is carried out after dissolution to form a stock solution, and then PH adjustment is carried out. Two conditioners are included and sprayed in the initial heading stage and the full heading stage respectively, glumous flower degeneration can be reduced, the photosynthetic production capacity of the upper three leaves after flowering can be improved, assimilation substances are promoted to be transferred to grains, especially the filling state of vulnerable grains is improved, and the yield of the seedlings is increased. Finally, the grain number, the maturing rate, the grain weight and the yield are improved, meanwhile, the head rice rate of rice can be improved, and the protein content is reduced, so that the palatability of rice is improved.

The invention relates to the technical field of flower flowering phase regulation and control, and discloses a regulator and a method for promoting secondary flowering of crape myrtle, the regulator comprises 50 parts by weight of benzylaminopurine, 4.6-5 parts by weight of compound sodium nitrophenolate and more than or equal to 100 parts by weight of an amino acid composition; or 18 parts by weight of benzylaminopurine, 18 parts by weight of gibberellin, 4.6-5 parts by weight of compound sodium nitrophenolate and more than or equal to 100 parts by weight of an amino acid composition. The regulator provided by the invention comprehensively acts on multiple aspects of cell division, dormancy breaking, hormone regulation, nutrition supply, stress resistance enhancement and the like to jointly promote secondary blooming of the crape myrtle, increase the blooming amount and prolong the blooming period, and by virtue of multiple aspects of effects of pruning, fertilization, insect killing, plant growth regulator regulation and the like, the yield of the crape myrtle is increased. Secondary blooming of the crape myrtle is promoted, the blooming rate, the blooming duration and the blooming amount of secondary blooming are increased, and the growth vigor of the crape myrtle in the next year is not affected.

A pharmaceutical composition including a nucleoside analogue loaded into liposomes of a liposomal delivery system, wherein the nucleoside analogue is a purine analogue chosen from {[2-(6-amino-9H-purin-9-yl)quinazolin-4-yl]oxy}phosphonic acid or 2-(6-amino-9H-purin-9-yl) quinazolin-4-ol, or a pyrimidine analogue chosen from 4-amino-1-(4-hydroxyquinazolin-2-yl)-1,2-dihydropyrimidin-2-one or N-[1-(4-hydroxyquinazolin-2-yl)-2-oxo-1,2-dihydropyrimidin-4-yl]acetamide.

The invention relates to the technical field of chrysanthemum breeding methods, in particular to a method for accelerating chrysanthemum breeding by using a liquid culture medium, which comprises the following steps: cutting chrysanthemum stalks into square blocks of 1cm * 1cm in a culture box, flatly laying the stalks upwards on an induction culture solution soaking base, cutting stem tissues with axillary buds into small sections of 2-3cm, and obliquely inserting the small sections into the induction culture solution for culturing, mS + 6-BA 2.0 mg / L +, NAA 0.1 mg / L, alpha-naphthylacetic acid 2-4 mg / L, 6-benzylaminopurine and cane sugar are selected as culture solutions, in the propagation culture of the chrysanthemum, stem seedling sections grow fast, each section generates a plurality of clustered seedlings, the average multiplication coefficient is 4-7 times, the height of the stem seedlings is different, the clustered seedlings are 5-7 cm high when the seedlings are cultured in the nutrient solution for 2 months, and the adventitious bud multiplication rate of the chrysanthemum seedlings is increased. The method has the effect of accelerating chrysanthemum reproduction.

The present invention relates to a composition for plant, comprising 6-benzyl aminopurine (6-BAP), a fatty acid as a solvent, and an emulsifier, to an emulsifiable concentrate formulation (EC) for producing an oil-in-water emulsifiable concentrate or an oil-in-water emulsifiable concentrate using the same, to a method for preparing the compositions, to a plant growth or development regulating method, or to a fruit thinning method.

The present application relates to the pharmaceutical use of 4-[9-(6-aminopurine)]-2(S)-hydroxybutyric acid methyl ester, in particular to its use in the preparation of a medicament for preventing and / or treating diseases associated with abnormal osteoclast activity. It has been found that 4-[9-(6-aminopurine)]-2(S)-hydroxybutyric acid methyl ester has a significant inhibitory effect on osteoclast differentiation, and intra-articular injection of 4-[9-(6-aminopurine)]-2(S)-hydroxybutyric acid methyl ester has a therapeutic effect on MIA-induced osteoarthritis. Therefore, 4-[9-(6-aminopurine)]-2(S)-hydroxybutyric acid methyl ester has the prospect of being developed into a therapeutic drug for diseases associated with abnormal osteoclast activity.

The invention discloses an underground propagule composite excitant for ecological restoration and a preparation method thereof, and relates to the technical field of excitant preparation. The fertilizer is prepared from the following raw materials in parts by weight: 0.2 to 0.3 part of gibberellin, 0.2 to 0.5 part of 6-benayl aminopurine, 0.2 to 0.3 part of indolebutyric acid, 0.5 to 0.7 part of sodium nitroprusside, 0.4 to 0.5 part of hydrogen peroxide, 1 to 2 parts of jasmonic acid sustained-release microspheres, 5 to 7 parts of compound amino acid, 1 to 3 parts of compound microbial agent sustained-release microspheres, 2 to 3 parts of seaweed extract, 10 to 13 parts of humic acid, 2 to 3 parts of monopotassium phosphate, 60 to 74 parts of ethanol and 1000 parts of water. The underground propagule composite excitant provided by the invention is beneficial to promoting activation and germination of underground propagules, enhancing plant growth and stress resistance, improving soil micro-ecological functions, forming stable diversity communities and facilitating ecological restoration.

The invention belongs to the technical field of medicine synthesis, and relates to a synthetic method of a fluorescent 2-aminopurine derivative. In particular to a synthetic method of 2-amino-6-cyanopurine, which is characterized in that cyanamide (SM-1) and 5-amino-4-(cyanoformyl imidogen) imidazole (SM-2) are used as raw materials to synthesize the 2-amino-6-cyanopurine. The problems of complex operation, long reaction time, unstable yield and the like in the prior art can be effectively avoided.

This invention discloses a culture medium for Antrodia camphorata mycelium, which consists of two parts: a solid medium and a liquid medium. The liquid medium contains glucose, peptone, anhydrous magnesium sulfate, and plant hormones; the plant hormones include one or more of indolebutyric acid, lignin, gibberellin, 6-benzylaminopurine, and 24-epibrassinolide. The solid medium contains carrot powder, potato powder, glucose, and agar powder; the solid medium also contains plant hormones, including one or more of indolebutyric acid, lignin, gibberellin, 6-benzylaminopurine, and 24-epibrassinolide. During cultivation, activated mycelium is first inoculated onto the solid medium to form high-quality colonies, and then inoculated onto the liquid medium for further expansion. This method significantly increases mycelium yield and the production of crude polysaccharides and crude triterpenes, providing strong technical support for the development and utilization of Antrodia camphorata.

The invention discloses a culture method for rapid rooting of gingko aseptic seedlings, and relates to the technical field of gingko tissue culture. The culture method for rapid rooting of the gingko aseptic seedlings comprises the following steps: inoculating the gingko aseptic seedlings into a first rooting culture medium for culture, and then transferring the gingko aseptic seedlings into a second rooting culture medium for culture, the second rooting culture medium is an MS culture medium containing 0.1 to 0.2 mg / L of 6-benayl aminopurine, 0.5 to 1.5 mg / L of indolebutyric acid, 15 to 25 mL / L of gingko sterile main root juice, 7 to 9 mL / L of gingko sterile cotyledon juice, 25 to 35 g / L of sucrose and 6 to 8 g / L of agar. The invention effectively solves the problems of lack of staged regulation and control, single culture medium component, poor functionality, unreasonable hormone proportion and the like in the prior art.

This invention discloses a method for in vitro preservation of monk fruit, comprising the following steps: inoculating monk fruit seedlings into an in vitro culture medium for in vitro preservation culture, wherein the in vitro culture medium comprises 1 / 2 MS, 0.01-0.03 mg / L 6-benzylaminopurine, 0.6-1.4 mg / L quinacrine, 0.05-0.15 mg / L dextrin, 0.2-0.8 g / L activated carbon, 0.3-0.7 mg / L white tea extract, 0.01-0.03 mg / L amino acids, 0.01-0.03 mg / L brassinolide, and 0.5-1.5 mg / L eucalyptus leaf infusion. This invention, by adding white tea extract and eucalyptus leaf infusion to the in vitro culture medium, can effectively extend the in vitro preservation time of monk fruit.

The invention relates to the technical field of saline-alkali soil improvement, in particular to a plant growth regulator for saline-alkali soil improvement and a preparation method thereof. The feed additive is prepared from the following components in parts by weight: 0.08 to 0.12 part of a modified regulator, 0.14 to 0.18 part of sucrose ester, 0.04 to 0.06 part of naphthylacetic acid, 0.12 to 0.18 part of gibberellic acid, 0.03 to 0.05 part of 6-benayl aminopurine, 0.8 to 1 part of vitamin C, 1 to 5 parts of mannitol, 1 to 3 parts of betaine and the balance of water, wherein the modification regulator is formed by surface modification of indoleacetic acid, sodium lignin sulfonate and millet colloid according to the mass ratio of 1: (5-10): (1.5-4.5); according to the plant growth regulator disclosed by the invention, the heteroauxin is wrapped and protected by the modified regulator through the millet colloid and the sodium lignin sulfonate, so that not only are the saline-alkali resistance and the plant growth promoting effect of the plant growth regulator improved, but also the plant growth regulator has a slow release effect, the action time of the plant growth regulator is prolonged, and stable growth stimulation is provided for plants; the use efficiency of the plant growth regulator is improved, and the use amount of the plant growth regulator and the influence on the environment are reduced.

The invention relates to a vase solution for preserving cut lily flowers of an OT hybrid line and application of the vase solution. The vase solution takes water as a solvent and comprises salicylic acid, citric acid, calcium chloride, 6-benayl aminopurine, aspirin, ascorbic acid, silver thiosulfate and cane sugar. The vase solution can improve the ornamental quality of cut flowers, remarkably prolong the service life of flowering branches and delay turbidity of the vase solution; in addition, the soluble sugar content and peroxidase content of the petals can be increased, and the malondialdehyde content of the petals can be reduced. The vase solution provided by the invention has the advantages of low cost, and simple and easy preparation and use.

The specification generally relates to compounds of Formula (I), or a stereoisomer or pharmaceutically acceptable salt thereof, wherein G, Ga, Gb, X, Y, R1, R2, Q1, Q2, and Q3 have any of the meanings defined herein, together with compositions containing them and their use in therapy. The compounds are inhibitors of the polymerase, DNA polymerase theta (Polθ or POLQ), and are thereby particularly useful in the treatment of cancer.

The invention relates to the technical field of culture medium manufacturing, in particular to a ford nervilia leaf tissue culture rhizome proliferation culture medium and a preparation method of the ford nervilia leaf tissue culture rhizome proliferation culture medium. The proliferation material is prepared from the following components in parts by weight: 2 to 4 parts of 6-benayl aminopurine, 0.3 to 0.7 part of naphthylacetic acid, 0.3 to 0.6 part of indolebutyric acid, 4 to 6 parts of gibberellin, 95 to 105 parts of novel coconut juice extract, 18 to 22 parts of novel pomegranate extract, 250 to 350 parts of cane sugar, 60 to 70 parts of agar and 3 to 5 parts of activated carbon. The improved pomegranate rind extract is subjected to ultrasonic and purification composite treatment, more antioxidant components such as ellagic acid and gallic acid are released, the antioxidant components, fermentation-enhanced amino acid and a nano silicon / selenium compound in the improved coconut juice extract form a dual antioxidant barrier, free radicals in plant cells are actively removed, oxidative damage is reduced, and the anti-aging effect is achieved. The hormone combination is precisely proportioned, so that lateral bud germination and stem elongation can be promoted.

The embodiment of the invention provides a golden camellia suspension cell culture method which comprises the following steps: golden camellia leaves are collected and inoculated into a WPM liquid culture medium containing hormone for culture, primary callus is formed through induction, and the hormone comprises naphthylacetic acid and 6-benayl aminopurine; and inoculating the primary callus into a WPM liquid culture medium containing auxin and cytokinin, and culturing to obtain the golden camellia suspension cells.

The invention belongs to the field of durian cultivation, and particularly relates to a durian flower forcing agent, a preparation method and application. According to the durian flower forcing agent, prohexadione calcium, potassium phosphite, benzyl benzyl aminopurine, thidiazuron and ethephon are reasonably combined, durian can be promoted to bloom in advance, the durian flower forcing agent is diluted and then sprayed to durian trees, budding of the durian trees can be achieved 9 days after spraying, and the budding time and the blooming time are shortened; meanwhile, the cluster number of durian flower buds can be remarkably increased, important guarantee is provided for increasing the yield of durian, and economic benefits of durian cultivation can be greatly increased.

The present invention belongs to the field of plant tissue culture technology, and specifically relates to a method and application for inducing callus tissue of aseptic monkey earring seedlings. The method comprises the following steps: taking the root system of the aseptic monkey earring seedlings as an explant and inoculating it in an induction medium for cultivation; the induction medium is an MS medium containing sucrose and agar, to which 0.4-0.6 mg / L of 6-benzylaminopurine, 0.1-0.5 mg / L of naphthaleneacetic acid, and 0-0.3 mg / L of gibberellins are added, and the pH is 5.8-5.9. The aseptic monkey earring seedlings cultivated by the present invention have lush branches and leaves, vigorous root growth, and have the advantages of convenient material collection and low contamination rate. The method can efficiently cultivate monkey earring root callus tissue, provide a reference for the asexual breeding of monkey earrings, and lay the foundation for inducing complete plants and realizing large-scale production of monkey earrings in the future.

The invention discloses a litsea cubeba secondary embryo proliferation culture medium and a proliferation culture method, the culture medium is based on an MS culture medium, cane sugar, agar, casein hydrolysate, 6-benayl aminopurine, naphthylacetic acid, inositol and cyclodextrin are added, and the pH value is 5.6-5.8; the method comprises the following steps: a somatic embryo generation stage, a secondary embryo proliferation stage and regular removal of mature somatic embryos. According to the method, the immature seeds are adopted as explants, through the somatic embryogenesis culture medium and the secondary embryo proliferation culture medium of a specific formula, dark culture and periodic subculture are combined, the proliferation efficiency of the secondary embryos is remarkably improved, the highest proliferation multiple can reach 20.17 times, meanwhile, the endophyte pollution risk is reduced, and the genetic stability is guaranteed. The method is simple and convenient to operate, short in culture period, low in cost and suitable for large-scale industrial application, provides important technical support for rapid propagation, germplasm preservation and industrial development of litsea cubeba, and has remarkable economic and social benefits.

The present invention discloses a method for isolating and culturing new, difficult-to-cultivate microbial species. A 6-dimethylaminopurine (6-DMAP) solution, sterilized by filtration through a PTFE filter, is added to a sterilized, liquid agar medium to a final 6-DMAP concentration of 1-3 μg / mL. The solution is then solidified to obtain a solid medium, which is then inoculated with a natural sample for cultivation. The method is highly efficient in isolating new microbial species, with approximately 50% of pure cultures yielding potential new species.

The invention belongs to the technical field of solar cell manufacturing, and provides a perovskite solar cell and a preparation method thereof. The perovskite solar cell comprises a conductive substrate, an electron transport layer, a perovskite layer, a passivation modification layer, a hole transport layer and an electrode layer. The perovskite layer is a perovskite layer modified by 2-aminopurine; and a passivation material in the passivation modification layer is 2-mercaptoethane-1-sulfonamide hydriodate. According to the invention, 2-aminopurine and 2-mercaptoethane-1-sulfonamide hydriodate are used for synergistically passivating the perovskite layer, so that the crystal defect and vacancy defect of the perovskite thin film are reduced, and the crystal quality of the perovskite thin film is improved, thereby improving the open-circuit voltage, short-circuit current density and energy conversion efficiency of the perovskite solar cell; and commercial application development of the perovskite solar cell is promoted.

The invention relates to a group of metabolic markers and application thereof in preparation of products for screening and / or diagnosing depression. The metabolic marker is prepared from one or more of 4-oxo-2-propyl valeric acid, diethylstilbestrol, cyclo (leucine-valine) peptide, 6-benzylaminopurine and 3-carboxyl-4-methyl-5-pentyl-2-furanpropionic acid, and the metabolic marker is prepared from one or more of the following components: 4-oxo-2-propyl valeric acid, diethylstilbestrol, cyclo (leucine-valine) peptide, 6-benzylaminopurine and 3-carboxyl-4 According to the application, research finds that 4-oxo-2-propyl valeric acid, diethylstilbestrol, cyclo (leucine-valine) peptide, 6-benzylaminopurine and 3-carboxyl-4-methyl-5-pentyl-2-furanpropionic acid can be used as metabolic markers, and high-sensitivity and high-specificity screening diagnosis is carried out on the depression.

The invention discloses a formula of a broad-spectrum culture medium adapted to sterile sowing of multiple species of Chinese orchid, and relates to the technical field of plant tissue culture, the broad-spectrum culture medium adopts a 1 / 2MS basic culture medium, the dosage of a macroelement mother solution is halved, and the dosage of ferric salt, microelement and vitamin mother solution is kept unchanged; 10 mg of modified graphene, 1.5 mg to 2.5 mg of 6-benayl aminopurine (6-BA), 0.3 mg to 0.8 mg of naphthylacetic acid (NAA), 0.2 mg to 0.8 mg of zeatin (ZT), 0.05 mg to 0.2 mg of indolebutyric acid (IBA), 1.2 g to 2.2 g of lactoalbumin hydrolysate, 0.6 g to 1.0 g of activated carbon, 22 g to 32 g of saccharose and 5.5 g to 7.5 g of agar are added into every 1 L of the basic culture medium. The pH value of the culture medium is adjusted to 5.2-5.8. By adding the modified graphene into the culture medium, germination of Chinese orchid seeds is effectively promoted. Meanwhile, a complete nutrition system and a root system induction mechanism enhance the growth vigor of the seedlings and improve the survival rate of the seedlings, and meanwhile the universality of the culture medium is remarkably improved through the formula of the culture medium.

The present invention provides oligonucleotides useful for the treatment of Duchenne muscular dystrophy or Becker muscular dystrophy, compositions containing oligonucleotides, and methods for prevention, treatment, and / or delay. [Solution] An oligonucleotide is provided that comprises a 2'-O-methylRNA monomer and a phosphorothioate skeleton, and also comprises 5-methyluracil and / or 5-methylcytosine and / or a 2,6-diaminopurine base.

The disclosure provides heterobifunctional substituted pyridinylmethyl aminopurine and related compounds, pharmaceutical compositions, their use for inhibiting NSD2 protein and / or inducing NSD2 protein degradation, and their use in the treatment of medical disorders, such as cancer.

The application provides a crystal form of a compound (2R, 3R, 4S)-2-(6-amino-9H-purin-9-yl)-2-(hydroxymethyl)-5-methylenetetrahydrofuran-3, 4-diol, which is selected from the crystal form F; wherein the crystal form F has characteristic diffraction peaks of an X-ray powder diffraction pattern expressed by 2θ values ± 0.2° using Cu-Kα radiation, and the characteristic diffraction peaks include 7.43, 13.07 and 13.69. The crystal form has good stability, can be kept stable under light, high temperature, high humidity and acceleration conditions, and can better meet the requirements of pesticides in production, processing, transportation and storage.

The application provides a kind of ophiopogon japonicus callus induction medium and its genetic transformation system construction method, belong to genetic transformation system construction technical field.A kind of ophiopogon japonicus callus induction medium is based on MS medium as base medium, further include 0.4~0.6 mg / L naphthalene acetic acid, 0.4~0.6 mg / L 2,4-D, 0.15~0.25 mg / L 6-benzylaminopurine, 28~32 g / L sucrose and 7~9 g / L agar, pH value is 5.8~6.0.The application utilizes the above-mentioned medium to carry out ophiopogon japonicus root callus induction culture, makes ophiopogon japonicus callus induction efficiency to 100%.Meanwhile, by optimizing the regeneration medium of callus, the regeneration rate reaches more than 90%.Based on two kinds of medium, the application establishes a kind of ophiopogon japonicus based on agrobacterium tumefaciens genetic transformation method, and the regeneration rate of transgenic seedling can reach more than 20%.

The application provides a crystal form of a compound (2R, 3R, 4S)-2-(6-amino-9H-purin-9-yl)-2-(hydroxymethyl)-5-methylenetetrahydrofuran-3, 4-diol, which is selected from crystal form D and crystal form E; wherein the characteristic diffraction peak of the X-ray powder diffraction pattern of the crystal form D using Cu-K alpha radiation, expressed by a 2 theta value ± 0.2°, includes 5.16, 7.26, 14.53 and 17.52; the characteristic diffraction peak of the X-ray powder diffraction pattern of the crystal form E using Cu-K alpha radiation, expressed by a 2 theta value ± 0.2°, includes 5.06, 7.18, 13.59 and 14.45. The crystal form has good stability and can maintain stability under light, high temperature, high humidity and acceleration conditions, and can better meet the requirements of pesticides in production, processing, transportation and storage.