35 results about "Parthenogenesis" patented technology

The invention discloses application of a gene CsPOD7 in cucumber haploid breeding, and belongs to the technical field of molecular breeding. According to the invention, a parthenogenesis haploid induction gene CsPOD7 is cloned from cucumber, the CsPOD7 is knocked out through a CRISPR-Cas9 gene editing technology, and a cspod7 mutant is used as a male parent to be hybridized with other cucumber materials as a female parent, so that parthenogenesis haploid can be generated. The invention is the first application of the gene CsPOD7 in developing a parthenogenesis haploid induction line in dicotyledon, realizes the application of the haploid induction line based on the gene CsPOD7 in cucumber genetic breeding, accelerates the cucumber breeding process, and has huge potential application value and market prospect.

The invention discloses an application of a gene CsDMP in cucumber haploid breeding. According to the invention, the parthenogenesis haploid induction gene CsDMP is cloned from cucumber. CsDMP is mutated by using a CRISPR-Cas9 gene editing technology, and an obtained csdmp mutant is used as a male parent to be hybridized with other cucumber materials, so that parthenogenetic haploids can be generated. The parthenogenesis haploid induction line is developed on cucumbers for the first time, application of the haploid induction line in cucumber genetic breeding is achieved, the cucumber breeding process is accelerated, and the application value and the market prospect are huge.

Provided herein are methods and compositions for inducing parthenogenesis by expression in female gametophytes of a Babyboom (BBM) polynucleotide operably linked to a promoter expressed in an egg cell. Also included herein are promoters expressed in egg cells of plants and methods of using these promoters to express gene products in egg cells of plants.

The invention discloses a method for cultivating kelp seedlings through diploid female gametophyte parthenogenesis, and belongs to the field of aquatic product engineering genetic breeding. And hybridizing the female and male single cell cloning lines of the induced protonema to obtain the tetraploid sporophyte. And the tetraploid sporophyte is mature and releases zoospores, diploid gametophytes are germinated and formed, and then male and female separation is performed to obtain diploid female gametophyte clone lines in batches. Under the culture condition provided by the invention, parthenogenesis of a diploid female gametophyte clone line is utilized for seedling culture. The diploid female gametophyte begins to ovulate after 6-7 days, and the ovulation time of the diploid female gametophyte is obviously earlier than that of haploid gametophyte in the same period and under the same condition. The 12-day ovulation rate reaches 86.5%, the 24-day sporophyte conversion rate reaches 98.3%, and most sporophyte forms are normal. In the seedling raising period, parthenogenesis seedling raising of the diploid female gametophyte is completed. The diploid female gametophyte can be used as a brand-new kelp germplasm material to be applied to kelp seedling cultivation.

The invention relates to the technical field of new silkworm variety breeding, and discloses a method for assisting silkworm variety breeding by parthenogenesis, which comprises the following steps: introducing a parthenogenesis high-incidence material into a target variety, screening individual hybridization recurrent parents retaining target characters or genes, and backcrossing with recurrent parent varieties, excellent individuals with target characters are selected for parthenogenesis homozygous immobilization and bisexual reproduction homozygous immobilization; according to the characteristic that a parthenogenesis high-incidence material is not easy to lose in filial generations due to the parthenogenesis high incidence, the parthenogenesis high-incidence material is hybridized, backcrossed and selfed with a target character variety and a conventional excellent variety, and after backcrossing is completed, parthenogenesis homozygous target character is carried out; target character homozygosis can be realized in a few generations, stable homozygous offspring can be obtained, and the breeding process is expected to be shortened.

Methods for improving parthenogenesis efficiency by DWT1 and BABY BOOM transcription factors in plants are provided. A rice embryo trigger transcription factor BABY BOOM1 can initiate embryogenesis when expressed in the unfertilized egg cell through a process called parthenogenesis (Khanday et al., 2019. Nature 565: 91-95). The parthenogenesis efficiency by BABY BBOM1 itself is 10-29%. This invention describes methods of high frequency of parthenogenesis by simultaneous expression of BABY BOOM and DWT1 transcription factors. When BABY BOOM1 and DWT1 are expressed together through egg cell-specific promoters, parthenogenesis efficiencies of up to 90% are achieved. These high parthenogenesis efficiencies are a prerequisite for field applications of synthetic apomixis in crop plants.

The invention discloses a genetically engineered kelp-based oral mucosal immune activation type HPV (human papilloma virus) vaccine as well as a preparation method and application thereof, and belongs to the technical field of manufacturing of genetically engineered vaccines. A kelp female gametophyte is modified by a genetic engineering method, parthenogenesis of the kelp female gametophyte is induced, a kelp plant stably expressing HPV L1 protein is obtained, and the oral mucosal immune activated HPV vaccine is prepared. According to the HPV vaccine provided by the invention, natural polysaccharide of kelp is used as an adjuvant and is absorbed through intestinal mucosa, so that the adverse reaction caused by traditional vaccine injection can be reduced while the mucosa immune reaction can be effectively activated. By utilizing the characteristic of rapid growth of the kelp and the advantage that the prepared vaccine does not need cold chain transportation, low-cost production and large-range coverage of the HPV vaccine can be realized, and global prevention and eradication of cervical cancer are expected to be promoted.

This invention discloses a gene for inducing parthenogenesis in dicotyledonous plants. ZbRWP Its applications, specifically, the parthenogenesis-inducing gene in dicotyledonous plants. ZbRWP This invention is the first to discover and verify its application in cultivating haploid or haploid induction lines of dicotyledonous plants or improving haploid induction ability. RWP This gene family possesses parthenogenetic haploid induction function in dicotyledonous plants, filling a gap in the list of parthenogenetic haploid induction genes in dicotyledonous plants. This invention lays an important foundation for broadening the application of haploid breeding technology in dicotyledonous plants and elucidating the biological mechanism of parthenogenetic haploid formation. Given the widespread use of haploid breeding technology in the current breeding industry, this invention has broad application prospects in the field of bio-breeding.

The application discloses a dicotyledon parthenogenesis induction gene BoRWP and application thereof, in particular a dicotyledon parthenogenesis induction gene BoRWP application in cultivating dicotyledon haploid or haploid induction line or improving haploid induction capacity, the application firstly discovers and verifies that RWP a gene family has parthenogenesis haploid induction function in dicotyledon, and fills the blank of dicotyledon parthenogenesis haploid induction gene. The application lays an important foundation for widening application of haploid breeding technology on dicotyledon and revealing biological mechanism of parthenogenesis haploid generation. In view of the wide use of haploid breeding technology in the current breeding industry, the application has wide application space and prospect in the field of biological breeding.

The invention relates to the technical field of transgenic bombyx mori pure linearization, and discloses a transgenic bombyx mori pure linearization method based on heat shock induced parthenogenesis, which comprises the following steps: S1, bombyx mori breeding and silkworm egg collection; s2, performing parthenogenesis induction treatment and subculture; s3, performing parthenogenesis condition investigation and data analysis. According to the heat shock induced parthenogenesis-based transgenic bombyx mori pure lineation method, animals with specific gene modification, such as animals capable of producing heterologous gene proteins, are bred by using a parthenogenesis method, pure lineation can be faster than natural mating breeding, and transgenosis bombyx mori is subcultured by using a parthenogenesis induction technology, so that the transgenic bombyx mori can be bred more quickly. According to the method, the process of hybridization breeding is avoided, the time of pure line is saved, the breeding cost is reduced, and a foundation is laid for improving the transgenosis efficiency, the genetic stability and the product safety.

The present invention relates to the technical field of new silkworm variety breeding, and discloses a method for assisting silkworm variety breeding by parthenogenesis. A material with a high incidence of parthenogenesis is introduced into the target variety, individuals with the target traits or genes are screened and retained as recurrent parents for hybridization, and they are backcrossed with the recurrent parent variety. Then, excellent individuals with the target traits are selected for homozygous fixation by parthenogenesis and homozygous fixation by bisexual reproduction. By utilizing the characteristic that the high incidence of parthenogenesis in the hybrid offspring is not easily lost when using the material with a high incidence of parthenogenesis, through hybridization, backcrossing and self-crossing of the material with a high incidence of parthenogenesis with the target trait variety and the conventional excellent variety, after the backcrossing is completed, the target traits are homozygously fixed by parthenogenesis, so that homozygosity of the target traits can be achieved within fewer generations, and stable homozygous offspring can be obtained, which is expected to shorten the breeding process.

To provide a procedure for inducing apomixis (also called agamospermy) in crops, and a method for producing an apomictic plant and an apomictic seed, and also provide a gene and a protein involved in the process of apomixis, particularly in parthenogenesis.SOLUTION: The invention provides nucleotide sequences and amino acid sequences of the parthenogenesis gene of Taraxacum as well as (functional) homologues, fragments and variants thereof, which provide parthenogenesis as a part of apomixis. Also parthenogenetic plants and methods for making these are provided, as are molecular markers and methods of using these.SELECTED DRAWING: None

The invention provides a kelp sex specific molecular marker and application thereof, wherein the nucleotide sequence of a male marker fragment is SEQ ID NO: 1, and the nucleotide sequence of a female marker fragment is SEQ ID NO: 2. A gene sequence fragment obtained by screening is from a conserved homologous gene in a sex determining region of brown algae. The screened sex identification marker primer sequence is from a region with the highest similarity in a homologous gene coding sequence, the sex of the kelp gametophyte can be rapidly identified only through a pair of degenerate primers, the efficiency is high, the accuracy is high, and the interspecific universality is high. The method is suitable for identification of male and female mixed samples, distinguishing of parthenogenesis source and sexual reproduction source kelp sporophytes and sex identification of gametophytes of other species of kelp, and important support is provided for efficient management and research and utilization of a kelp gametophyte germplasm bank.

The invention provides a method to produce a mutant gene, wherein said gen comprises a modified promoter and wherein said gene is capable of inducing the parthenogenesis phenotype to a plant. The invention further provides said mutant gene, isolated nucleic acid molecule, construct or vector comprising the same. Also, the invention provides for a method to produce a parthenogenetic plant comprising the mutant gene, and the parthenogenetic plant thus obtained.

The invention discloses a dicotyledonous parthenogenesis induction gene RWP and application thereof, and particularly relates to application of the dicotyledonous parthenogenesis induction gene RWP in cultivation of dicotyledonous haploid or haploid induction lines or improvement of haploid induction capability. The invention discovers and verifies that the RWP gene family has the parthenogenesis haploid induction function in the dicotyledon for the first time, and fills the blank of the dicotyledon parthenogenesis haploid induction gene. The invention lays an important foundation for widening the application of haploid breeding technology on dicotyledonous plants and revealing the biological mechanism of parthenogenesis haploid production. In view of the universality of haploid breeding technology utilization in the current breeding industry, the invention has wide application space and prospect in the field of biological breeding.

The invention belongs to the technical field of biology, and particularly relates to a method for cultivating obligate parthenogenesis rotifers based on a gene editing technology and application of the method in preparation of novel aquatic breeding initial feed. The application is obtained by combining brand new rotifer microinjection with gene knockout rotifer mutant screening, and a new obligate parthenogenesis rotifer variety prepared by the application can be used as a novel living body bait for aquaculture and seedling raising.

The present invention relates to the field of biotechnology, and in particular to a sheep parthenogenetic haploid stem cell culture medium and its application. Sheep mature oocytes are parthenogenetically activated to obtain sheep parthenogenetic embryos, the sheep parthenogenetic embryos are cultured in vitro to blastocysts, and then the sheep parthenogenetic blastocysts are cultured in culture medium to obtain sheep parthenogenetic haploid stem cells. The culture medium includes mTeSR1 basal culture medium and FGF2, Activin A, CHIR99021 and XAV939, and each 500 mL mTeSR1 basal culture medium contains 5‑10 ng / mL FGF2, 0.5‑1 μM Activin A, 1‑2.5 μM CHIR99021, and 0.5‑1 μM XAV939. The present invention provides a culture medium suitable for sheep parthenogenetic haploid stem cell culture, obtains sheep parthenogenetic haploid stem cells, and establishes a sheep parthenogenetic haploid stem cell cell line. The entire genome of sheep parthenogenetic haploid stem cells is derived from oocytes, and they have the self-replication ability and pluripotency of stem cells. The resulting cell line is a haploid cell line and can be used for genetic screening at the cellular level.

The invention discloses a genetic sex-specific molecular marker covering seven InDel sites of a spinibarbus hollandi male and application of the genetic sex-specific molecular marker covering the seven InDel sites of the spinibarbus hollandi male. The molecular marker successfully recognizes an inter-sex specific insertion or deletion sequence (InDel) through whole genome re-sequencing in combination with a whole genome association analysis method. Polymerase chain reaction (PCR) amplification is carried out through a conventional primer, and in combination with an agarose gel electrophoresis technology, rapid and stable identification of hereditary sex of spinibarbus hollandi is realized. The molecular marker covers seven InDel loci of the spinibarbus hollandi male, shows the advantages of simplicity and convenience in operation and accurate result in the aspect of genetic sex identification, has important significance in researching the sex determination mechanism of the spinibarbus hollandi, and can provide assistance for a parthenogenesis technology, so that the economic benefit of aquaculture is improved.

Methods and compositions disclosed herein relate to genes involved in plant production and methods of using the same.

Provided are a parthenogenetic haploid induction gene DMP and an application thereof. The parthenogenetic haploid induction genes AtDMP8 and AtDMP9 are cloned from Arabidopsis thaliana. Experiments have shown that mutations of AtDMP8 and AtDMP9 can produce parthenogenetic haploid inducibility, to enable dicotyledonous crops to be induced to produce haploids via parthenogenetic means. The present invention was further verified in tomatoes, and it was also found in tomatoes that the mutation of SlDMP can produce parthenogenetic haploid inducibility. The invention lays an important foundation for broadening the application of haploid breeding technology on dicotyledonous plants and revealing the biological mechanism of parthenogenetic haploid production. Given the universality of the utilization of haploid breeding technology in the current breeding industry, the invention has very wide application space and market prospects.

The present invention provides the nucleotide and amino acid sequences of parthenogenesis genes in the genus Taraxacum and their (functional) homologues, fragments and variants thereof, which provide parthenogenesis as part of apomixis. Also provided are parthenogenetic plants and methods for preparing them, as well as molecular markers and methods for using them.

The application provides a method for genetic reactivation of individual Procyprinus palpebrosus, comprising the following steps: S1, taking the gonadal tissue of Procyprinus palpebrosus, washing, cutting, adding L-15 culture medium containing double antibodies and DPBS buffer solution to centrifuge the gonadal tissue; S2, water-bathing the gonadal tissue to obtain recovered gonadal tissue; S3, digesting the recovered gonadal tissue, filtering, centrifuging and purifying to obtain germ stem cells; S4, injecting morpholino to obtain rare gobi-ocypris rarus with deleted primordial germ cells; S5, transplanting the germ stem cells into the body of the rare gobi-ocypris rarus juvenile to culture, and obtaining the gametes of Procyprinus palpebrosus; and S6, fertilizing the generated gametes of Procyprinus palpebrosus to obtain the individual Procyprinus palpebrosus. The application successfully obtains the sperm of Procyprinus palpebrosus by using the cryopreserved gonadal tissue and the technology of parthenogenesis, and provides a feasible way for the individual genetic reactivation of rare and endangered fish species.

This invention provides a sex-specific molecular marker for kelp and its application, wherein the nucleotide sequence of the male marker fragment is SEQ ID NO:1, and the nucleotide sequence of the female marker fragment is SEQ ID NO:2. The gene sequence fragments obtained by this invention are derived from conserved homologous genes in the sex-determining regions of brown algae. The primer sequences for the selected sex identification markers are derived from the regions with the highest similarity in the coding sequences of the homologous genes. This method enables rapid identification of the sex of kelp gametophytes using only a pair of degenerate primers, offering high efficiency, high accuracy, and high interspecies compatibility. This method is applicable to the identification of mixed male and female samples, the differentiation of kelp sporophytes from parthenogenesis and sexual reproduction, and the sex identification of gametophytes in other species of the Laminariales order, providing important support for the efficient management and research utilization of kelp gametophyte germplasm banks.

The present invention discloses a method for cultivating double haploid of fragrant green vegetable by inducing parthenogenesis through distant hybridization. Using non-heading Chinese cabbage 'Fragrant Green Vegetable' as the female parent and broad bean 'Japanese Big White Skin' as the male parent, artificial pollination hybridization is carried out, and brassinolide solution is sprayed to induce parthenogenesis, so as to cultivate the double haploid of fragrant green vegetable. As long as artificial cross-pollination is carried out and combined with the application of brassinolide solution, the double haploid of fragrant green vegetable can be obtained, which can accelerate the rate of cultivating high-quality germplasm resources of fragrant green vegetable.

This invention discloses a gene for inducing parthenogenesis in dicotyledonous plants. RWP Its applications, specifically, the parthenogenesis-inducing gene in dicotyledonous plants. RWP This invention is the first to discover and verify its application in cultivating haploid or haploid induction lines of dicotyledonous plants or improving haploid induction ability. RWP This gene family possesses parthenogenetic haploid induction function in dicotyledonous plants, filling a gap in the list of parthenogenetic haploid induction genes in dicotyledonous plants. This invention lays an important foundation for broadening the application of haploid breeding technology in dicotyledonous plants and elucidating the biological mechanism of parthenogenetic haploid formation. Given the widespread use of haploid breeding technology in the current breeding industry, this invention has broad application prospects in the field of bio-breeding.

The invention provides a novel method for realizing apomixis of plants. By removing egg cell expression inhibition factors of endogenous parthenogenesis genes (including BBM1, BBM4, CPRO1, CPRO2, WUS and the like) of rice and combining with a MiMe strategy, a novel apomixis method which only depends on gene editing is realized. According to the method, a gene editing means is adopted completely, an egg cell ectopic expression technology is not involved, apomixis is achieved by removing an egg cell expression inhibition factor of a rice endogenous parthenogenesis gene, the purpose of not involved transgenosis is achieved, and production and application of an artificial apomixis technology in crops are achieved.

The present invention discloses a method for cultivating kelp seedlings using parthenogenesis of diploid female gametophytes, belonging to the field of genetic breeding in aquatic engineering. Female and male single-cell clones of induced conchoderma are hybridized to obtain tetraploid sporophytes. The tetraploid sporophytes mature and release zoospores, which germinate to form diploid gametophytes. Male and female gametophytes are then separated and batches of diploid female gametophyte clones are obtained. Under the culture conditions provided by the present invention, seedlings are cultivated using parthenogenesis of the diploid female gametophyte clones. These diploid female gametophytes begin to ovulate within 6 to 7 days, significantly earlier than the ovulation time of haploid gametophytes under the same conditions during the same period. The ovulation rate reaches 86.5% on day 12, and the sporophyte conversion rate reaches 98.3% on day 24. Most of the sporophytes have normal morphology. During the seedling cultivation period, the diploid female gametophytes complete parthenogenesis. The diploid female gametophytes can be used as a new kelp germplasm material for kelp seedling cultivation.

This invention discloses a method for altering the reproductive pattern of *Trichogramma pseudooligophthalmus*. The method includes treating *Trichogramma pseudooligophthalmus* that is fully engaged in parthenogenesis with a rifampicin-honey aqueous solution at a concentration of 0.1-0.5 mg / ml. After treatment, the *Trichogramma pseudooligophthalmus* is used to parasitize brown planthopper eggs to obtain male F1 generation *Trichogramma pseudooligophthalmus*. This invention provides a model for exploring the reproductive methods of *Wolbachia* in hymenoptera, lays the foundation for further exploration of the reproductive patterns of *Trichogramma pseudooligophthalmus*, and also provides a model for the removal of endosymbiotic bacteria in other arthropods.

The present invention belongs to the fields of biotechnology and plant breeding. Specifically disclosed are a gene having parthenogenesis-inducing ability in plants, and the use thereof in plant breeding such as induction of haploid formation and apomixis. By means of regulating the expression of a gene that encodes a protein having parthenogenesis-inducing ability, the transition of egg cells into the embryonic development fate is effectively realized, thereby inducing parthenogenesis. The gene and a corresponding induction method therefor can be applied to related plant breeding processes such as the production of haploids and synthetic apomixis.